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Method for identifying corn parthenogenesis haploidy

A parthenogenetic and haploid technology is applied in the field of corn kernel oil content as a marker to identify corn parthenogenetic haploid, which can solve the problems of large differences in human and material resources, identification and marker expression, and improve oil content. , Simple operation, high identification efficiency

Inactive Publication Date: 2009-03-04
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practice, it has been found that the genotype selectivity of this marker system is relatively strong, and the marker expression of different materials varies greatly, and some materials can hardly be identified by grain Navajo markers; and different environmental conditions have a great influence on the expression of markers
In addition, using grain color to identify haploids requires a lot of manpower and material resources

Method used

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  • Method for identifying corn parthenogenesis haploidy
  • Method for identifying corn parthenogenesis haploidy
  • Method for identifying corn parthenogenesis haploidy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, the ploidy effect of haploid oil part

[0063] 1. Experimental materials

[0064] Male parent: maize parthenogenic haploid induction line Stock6 (Coe E H.A line of maize with high haploid frequency [J]. Am Nat, 1959, 93:381-382) (China Agricultural University), oil content 2.95%;

[0065] Female parent: hybrid Nongda 108 (Beijing Golden Nonghua Seed Industry Technology Co., Ltd.), hybrid Zhengdan 958 (Beijing Denong Seed Industry Co., Ltd.), hybrid Xianyu 335 (American Pioneer Company), hybrid ND5598 (Li Liang, C.Friedrich H.Longin, Sun Wentao, etc. Estimation of Genetic Parameters of Nitrogen Use Efficiency in Summer Maize in the North China Plain[J]. Journal of China Agricultural University, 2007, 12(6):50-56) (high oil variety, oil content about 6.5%) (China Agricultural University);

[0066] 2. Induction of haploid and identification:

[0067] 1. Haploid induction

[0068] The experiment was designed in randomized blocks, and the following 4 treatm...

Embodiment 2

[0082] Embodiment 2, the pollen intuition effect of high oil type inducer oil part

[0083] The pollen intuition effect of oil content refers to the ability to increase the oil content of diploid kernels in contemporary hybrids when high-oil materials are used as male parents.

[0084] 1. Experimental materials

[0085] Male parent (i.e. induced line): High-oil type induced line Nongda Gaoyu No. 1 (Liu Zhizeng, Song Tongming. Breeding and identification of high-frequency parthenogenic haploid induction line in maize. Acta Crops, 2000, 26(5): 570-574) (China Agricultural University);

[0086] Female parents: 178, 4F1, Chang 7-2, Dan 598, Zheng 58, all from the National Crop Germplasm Bank of the Institute of Crop Variety Resources, Chinese Academy of Agricultural Sciences.

[0087] 2. Experimental method:

[0088] The experimental design was a completely randomized block design, with a total of 10 treatments as follows.

[0089] 1. Haploid induction treatment: 5 panicles we...

Embodiment 3

[0105] Embodiment 3, utilize oil content to carry out haploid identification

[0106] 1. Experimental materials

[0107] Female parent: hybrid Zhengdan 958 (Beijing Denong Seed Industry Co., Ltd.); male parent: high-oil type induction line Nongda Gaoyu No. 1 (Liu Zhizeng, Song Tongming. Breeding of high-frequency parthenogenetic haploid induction lines in maize Journal of Crops and Identification, 2000, 26(5): 570-574) (China Agricultural University).

[0108] 2. Experimental method of haploid induction

[0109] Zhengdan 958 was planted in the field, and the haploid induction line Nongda Gaoyu 1 was planted at staggered dates. Gaoyu 1 was used as the male parent to cross 15 ears with Zhengdan 958, and Zhengdan 958 selfed 2 ears at the same time. Inbred contemporary kernels and air dry. A total of 5,000 hybrid seeds and 500 self-bred seeds were harvested.

[0110] 3. Haploid Identification

[0111] (1) Haploid identification of hybrid seeds according to the color marking o...

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Abstract

The invention discloses a method for identifying a core parthenogenesis haploid. The method for identifying the core parthenogenesis haploid comprises the following steps: (1) a corn haploid derived line is used to be taken as a male parent to carry out hybridization with a female parent corn, and hybrid present kernals are harvested; (2) an identification standard value is ensured; (3) the oil content of a single hybrid present kernal to be tested is tested, if the oil content of the single hybrid present kernal to be tested is bigger than or equal to the identification standard value, the single hybrid present kernal to be tested is the haploid. Compared with the traditional method, the identification method has the characteristics of convenient operation, time conservation, labor conservation and high identification efficiency. The identification method opens up a new way for the identification of the haploid, can carry out large scale operation, and provides the possibility for carrying out haploid picking in a mechanized way, thereby enhancing the breeding efficiency.

Description

technical field [0001] The invention relates to a method for identifying maize parthenogenetic haploids, in particular to a method for identifying maize parthenogenetic haploids using the oil content of corn kernels as a marker. Background technique [0002] Maize is a monoecious, sexually reproducing crop. The maize breeding work mainly utilizes maize sexual reproduction to select maize inbred lines, prepare maize combinations, and reproduce maize hybrids. However, in the process of cultivating maize inbred lines, it must go through multiple generations of selfing and selection. It usually takes ten years to breed a variety, which consumes a lot of manpower, material resources and financial resources. Therefore how to shorten the breeding process and improve the breeding efficiency is the goal that breeders have been pursuing. [0003] Maize haploid is the product of gametophyte apomixis, including parthenogenesis and parthenogenesis. By artificially doubling the obtain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/02
Inventor 陈绍江黎亮
Owner CHINA AGRI UNIV
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