17 results about "Apomixis" patented technology

This invention provides the nucleotide and amino acid sequences of the Dip gene, as well as its (functional) homologs, fragments, and variants, which provide ploid sporophyte formation as part of apomixis. It also provides ploid sporophyte-forming plants, methods for their preparation, methods for using them, and methods for preparing apomixis seeds.

Methods for improving parthenogenesis efficiency by DWT1 and BABY BOOM transcription factors in plants are provided. A rice embryo trigger transcription factor BABY BOOM1 can initiate embryogenesis when expressed in the unfertilized egg cell through a process called parthenogenesis (Khanday et al., 2019. Nature 565: 91-95). The parthenogenesis efficiency by BABY BBOM1 itself is 10-29%. This invention describes methods of high frequency of parthenogenesis by simultaneous expression of BABY BOOM and DWT1 transcription factors. When BABY BOOM1 and DWT1 are expressed together through egg cell-specific promoters, parthenogenesis efficiencies of up to 90% are achieved. These high parthenogenesis efficiencies are a prerequisite for field applications of synthetic apomixis in crop plants.

The application discloses Zanthoxylum bungeanum miR167b The application belongs to the technical field of genetic engineering, and discloses a gene in the application of regulating plant growth and development miR167b The application constructs an expression vector of the gene, and makes the gene overexpress in tomatoes, and finds that miR167b The overexpression plant is dwarfed, the shape of the fruit is changed, the size and weight of the fruit are significantly lower than those of the wild type, the fruit setting rate is high, the fruit is seedless, the number of fruit chambers is reduced, ovule abortion occurs, and no embryo sac is formed in the interior. Further analysis finds that the indole butyric acid content in the fruit of the overexpression plant is increased, which indicates that miR167b The gene can regulate auxin to induce fruit apomixis, lays a foundation for revealing the apomixis mechanism of Zanthoxylum bungeanum, and provides gene resources and basis for improving the breeding strategy of woody plants, and has important theoretical value and application prospect.

The application relates to the field of plant breeding, and specifically provides a method for fixing hybrid vigor of rice by using an OsMPF gene. An expression cassette of an OsECA1 promoter driven OsMPF gene is first constructed; an expression cassette of three target CRISPR / Cas9 knockout of rice OsPAIR1, OsREC8 and OsOSD1 is then constructed; the above expression cassettes are integrated into the same vector to transform hybrid rice; three-gene homozygous mutant and OsMPF positive plants are screened, and diploid clone offspring are identified through flow cytometry and genome sequencing. The method provides a new method for fixing hybrid vigor of apomixis of rice by combining the rice OsMPF gene with MiMe, the method can successfully fix the hybrid vigor of rice, can obtain an apomixis system with high seed setting rate, and provides a new solution for fixing hybrid vigor of rice apomixis.

The application discloses genes for inducing apomixis of plants, an inducing method formed by the genes and application, and relates to the fields of biotechnology and plant breeding. AtCPRO1, genes in corn Zm CPRO3, Zm CPRO4, genes in soybean GlCPRO2, genes in rape Bna CPRO8 are respectively introduced into rice, and then, by allowing the genes to ectopically express in oocytes, the genes are combined with MiMe body ​ Under the premise of not affecting growth and development of the plants, the genes can induce the generation of clonal diploid offspring, and provide a new solution for rice breeding, and have important application prospect and economic value.

The present invention relates to nucleic acid constructs comprising the coding sequence of the apomictic trait of Eragrostis curvula ( Eragrostis curvula ) and the use of these constructs for the transformation of non-apomictic plants.

A method for producing 100% sexual cultivars of a grass plant developed from facultative apomictic germplasm. Sexual cultivars of Kentucky bluegrass include those of breeder's codes: AKB020, AKB413, AKB419, AKB484, AKB357, and AKB397. Additionally, disclosed is a method for producing hybrid apomictic plants from hybridization of a completely sexual parent with a facultative or apomictic parent, including those of breeder's codes AKB1661, AKB1658, AKB3605, AKB2839, AKB2949, AKB3128, AKB3179, AKB3201, AKB3216, AKB3241.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsMPF gene. The method comprises the following steps: firstly, constructing an OsMPF gene expression box driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsMPF positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsMPF gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through apomixis.

Methods and compositions disclosed herein relate to genes involved in plant production and methods of using the same.

A method for producing 100% sexual cultivars of a grass plant developed from facultative apomictic germplasm. Sexual cultivars of Kentucky bluegrass include those of breeder's codes: AKB020, AKB413, AKB419, AKB484, AKB357, and AKB397. Additionally, disclosed is a method for producing hybrid apomictic plants from hybridization of a completely sexual parent with a facultative or apomictic parent, including those of breeder's codes AKB1661, AKB1658, AKB3605, AKB2839, AKB2949, AKB3128, AKB3179, AKB3201, AKB3216, AKB3241.

The invention discloses a character-stable Chinese holly propagation method based on an apomixis phenomenon, and relates to the technical field of Chinese holly breeding and propagation. According to the method, fine flower thinning is carried out on screened female ilex cornuta plants with excellent characters before flowering of the female ilex cornuta plants; bagging and isolating immediately after flower thinning so as to completely eradicate external pollen pollution; after fruit setting, the bagging type is changed, and a protective agent is sprayed, so that physiological fruit falling is prevented; and finally, harvesting seeds and sowing to obtain a clonal offspring with the genetic character highly consistent with that of the female parent. According to the method, bagging isolation and physiological regulation are combined, the apomixis characteristic of the ilex cornuta is effectively utilized, and breeding with stable characters of excellent single plants is achieved. The method has the remarkable advantages of simplicity and convenience in operation, low cost, high propagation efficiency and stable progeny characters, and is suitable for new Chinese holly variety breeding, rapid propagation and new plant variety protection.

The invention provides a novel method for realizing apomixis of plants. By removing egg cell expression inhibition factors of endogenous parthenogenesis genes (including BBM1, BBM4, CPRO1, CPRO2, WUS and the like) of rice and combining with a MiMe strategy, a novel apomixis method which only depends on gene editing is realized. According to the method, a gene editing means is adopted completely, an egg cell ectopic expression technology is not involved, apomixis is achieved by removing an egg cell expression inhibition factor of a rice endogenous parthenogenesis gene, the purpose of not involved transgenosis is achieved, and production and application of an artificial apomixis technology in crops are achieved.

The application provides a binary expression vector for fixing soybean hybrid advantage and a construction method and application thereof, and belongs to the field of plant breeding and biotechnology. The binary expression vector is obtained by one-time construction of a CRISPR / Cas9-mediated soybean MiMe system and a ToPAR gene, and is specifically started by a soybean oocyte-specific promoter pGmEC1.1. The vector is delivered to a soybean hybrid by using an agrobacterium-mediated soybean cotyledon node genetic transformation system, and a material with full mutation of five genes of the MiMe and specific expression of the ToPAR gene is screened, so that apomixis is successfully induced in the dicotyledonous crop soybean, the pairing efficiency of hybrid soybean is improved, and the seed production risk of hybrid soybean is eliminated.

The present invention belongs to the fields of biotechnology and plant breeding. Specifically disclosed are a gene having parthenogenesis-inducing ability in plants, and the use thereof in plant breeding such as induction of haploid formation and apomixis. By means of regulating the expression of a gene that encodes a protein having parthenogenesis-inducing ability, the transition of egg cells into the embryonic development fate is effectively realized, thereby inducing parthenogenesis. The gene and a corresponding induction method therefor can be applied to related plant breeding processes such as the production of haploids and synthetic apomixis.

In contrast to meiosis, where meiotic recombination, random segregation of chromosomes and ploidy reduction occurs, Mitosis instead of Meiosis (MiMe), an engineered form of apomeiosis, leads to unreduced clonal gametes in the Solanaceae (night-shades), and, via selfing, to tetraploid offspring that stably inherit genome-wide heterozygosity and plant phenotypes. In the MiMe system, factors involved in meiotic recombination, sister chromatid cohesion, and meiosis II entry are knocked out or knocked down. Engineered clonal unreduced gamete formation by MiMe can be used as a component of synthetic apomixis, and to develop polyploid genome design.

The invention discloses a method for producing rice haploids and application of the method in breeding, by editing one or more of OsFEB1, OsFEB2 and OsFEB3 genes in rice, the functions of the OsFEB1, OsFEB2 and / or OsFEB3 genes are lost, a rice haploid induction line is obtained, generation of haploids is induced through sexual reproduction, the gene sequence of the OsFEB1 is as shown in SEQ ID NO.1, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.2, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.1, the gene sequence of the OsFEB2 is as shown in SEQ ID NO.2, and the gene sequence of the OsFEB3 is as shown in SEQ ID NO.2. And the gene sequence of the OsFEB3 is as shown in SEQ ID NO. 3. The haploid induction line obtained by the method provided by the invention has the capability of inducing generation of haploid plants for breeding. An RHG1 haploid induction line is combined with an MIME system, and an apomixis material is artificially created to fix the heterosis of rice.

The invention belongs to the field of biotechnology and plant breeding, and particularly discloses a gene with parthenogenesis induction capacity in plants and application of the gene in plant breeding such as haploid formation induction and apomixis induction. According to the present invention, the expression of the gene coding the protein with the parthenogenesis induction ability is regulated, such that the change from the egg cell to the embryonic development fate is effectively achieved so as to induce parthenogenesis; the gene and the corresponding induction method thereof can be applied to related plant breeding processes such as haploid production and apomixis synthesis.