33 results about "Apomixis" patented technology

The invention relates to the field of biotechnology and plant breeding, and particularly provides a method for fixing rice heterosis by using a DMP gene. Aiming at the problems of low maturing rate and complex operation of the existing apomixis system, the haploid induction functions of the rice OsDMP8 and OsDMP13 genes are verified for the first time, the OsPAIR1, OsREC8, OsOSD1 and OsDMP genes are synchronously knocked out through a CRISPR / Cas9 technology, a'MiMe-OsDMP 'four-gene editing system is constructed, and a new thought is provided for construction of the rice apomixis system. A new solution is provided for fixing heterosis of rice apomixis, the efficiency and quality of agricultural production can be greatly improved, and the method has high economic value and wide application prospects.

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

The invention discloses application of a ZaSPL7 gene in regulation and control of hairy bodies and parthenocarpy of plants, and belongs to the technical field of gene engineering. The gene ZaSPL7 for regulating and controlling organ development of the green prickleyash is separated from cDNA of the green prickleyash and is overexpressed in a plant, the number and density of epidermal hair of the transgenic plant are obviously increased, meanwhile, the number of fruit seeds is obviously reduced, even part of plants have seedless fruit phenotypes, and the unisexual setting rate is increased. The results show that the green prickleyash SPL7 gene may coordinate epidermal hair and fruit development by integrating development stage signals, and has important application value in regulation and control of epidermal appendages (such as thorns and hair) formation and apomixis, and the mechanism provides a new idea for simultaneous improvement of plant stress resistance (by reducing the thorns) and fruit commodity (by seedless). Important theoretical basis and technical support are provided for breeding of the green prickleyash, and the method has a wide application prospect.

The invention discloses a ZaSCL3 gene for regulating and controlling plant growth and development as well as an encoding protein and application thereof, and belongs to the technical field of gene engineering. According to the invention, an over-expression vector of the ZaSCL3 gene is constructed and expressed in a wild tomato, so that stamens of a ZaSCL3 over-expression plant are aborted, ovary is spontaneously expanded without pollination, and seedless fruits are generated, which indicates that the over-expression ZaSCL3 gene can realize the effect of inducing male sterility and apomixis of the plant; a key gene resource is provided for molecular breeding and yield character improvement, and the important application value is achieved.

This invention provides the nucleotide and amino acid sequences of the Dip gene, as well as its (functional) homologs, fragments, and variants, which provide ploid sporophyte formation as part of apomixis. It also provides ploid sporophyte-forming plants, methods for their preparation, methods for using them, and methods for preparing apomixis seeds.

The invention discloses a gene for inducing apomixis of plants, an inducing method formed by the gene and application of the gene. Relates to the field of biotechnology and plant breeding. The genes for inducing the apomixis of the plants are respectively arabidopsis thaliana AtCPRO1, corn Zm CPRO3, corn Zm CPRO4, soybean GlCPRO2 and rape Bna CPRO8. According to the induction method of the gene composition, AtCPRO1 in arabidopsis thaliana, Zm CPRO3 and Zm CPRO4 in corn, GlCPRO2 in soybean and Bna CPRO8 in oilseed rape which exist in the nature are introduced into rice respectively, ectopic expression is performed in egg cells, then combination with a MiMe system is performed, on the premise that growth and development of plants are not affected, generation of cloned diploid progeny can be induced, and the yield of the cloned diploid progeny is increased. The invention provides a new solution for rice breeding, and has important application prospect and economic value.

Methods for improving parthenogenesis efficiency by DWT1 and BABY BOOM transcription factors in plants are provided. A rice embryo trigger transcription factor BABY BOOM1 can initiate embryogenesis when expressed in the unfertilized egg cell through a process called parthenogenesis (Khanday et al., 2019. Nature 565: 91-95). The parthenogenesis efficiency by BABY BBOM1 itself is 10-29%. This invention describes methods of high frequency of parthenogenesis by simultaneous expression of BABY BOOM and DWT1 transcription factors. When BABY BOOM1 and DWT1 are expressed together through egg cell-specific promoters, parthenogenesis efficiencies of up to 90% are achieved. These high parthenogenesis efficiencies are a prerequisite for field applications of synthetic apomixis in crop plants.

The application discloses Zanthoxylum bungeanum miR167b The application belongs to the technical field of genetic engineering, and discloses a gene in the application of regulating plant growth and development miR167b The application constructs an expression vector of the gene, and makes the gene overexpress in tomatoes, and finds that miR167b The overexpression plant is dwarfed, the shape of the fruit is changed, the size and weight of the fruit are significantly lower than those of the wild type, the fruit setting rate is high, the fruit is seedless, the number of fruit chambers is reduced, ovule abortion occurs, and no embryo sac is formed in the interior. Further analysis finds that the indole butyric acid content in the fruit of the overexpression plant is increased, which indicates that miR167b The gene can regulate auxin to induce fruit apomixis, lays a foundation for revealing the apomixis mechanism of Zanthoxylum bungeanum, and provides gene resources and basis for improving the breeding strategy of woody plants, and has important theoretical value and application prospect.

The present invention relates to nucleic acid constructs comprising a coding sequence for apomictic traits of Eragrostis curvula, and their use for transforming non-apomictic plants.

The present application relates to the field of plant breeding, and specifically provides a method for fixing hybrid vigor of rice by using OsZFPP gene. First, an expression cassette of OsECA1 promoter driven OsZFPP1 gene or OsZFPP2 gene is constructed; then, an expression cassette of three target CRISPR / Cas9 knockout of rice OsPAIR1, OsREC8 and OsOSD1 is constructed; the above expression cassettes are integrated into the same vector to transform hybrid rice; three-gene homozygous mutant and OsZFPP positive plants are screened, and diploid clone offspring is identified by flow cytometry and genome sequencing. The method provides a new method for fixing hybrid vigor of apomixis of rice by combining rice OsZFPP gene with MiMe, which can successfully fix hybrid vigor of rice, can obtain high seed setting rate apomixis system, and provides a new solution for fixing hybrid vigor of rice apomixis.

The application relates to the field of plant breeding, and specifically provides a method for fixing hybrid vigor of rice by using an OsMPF gene. An expression cassette of an OsECA1 promoter driven OsMPF gene is first constructed; an expression cassette of three target CRISPR / Cas9 knockout of rice OsPAIR1, OsREC8 and OsOSD1 is then constructed; the above expression cassettes are integrated into the same vector to transform hybrid rice; three-gene homozygous mutant and OsMPF positive plants are screened, and diploid clone offspring are identified through flow cytometry and genome sequencing. The method provides a new method for fixing hybrid vigor of apomixis of rice by combining the rice OsMPF gene with MiMe, the method can successfully fix the hybrid vigor of rice, can obtain an apomixis system with high seed setting rate, and provides a new solution for fixing hybrid vigor of rice apomixis.

The application discloses genes for inducing apomixis of plants, an inducing method formed by the genes and application, and relates to the fields of biotechnology and plant breeding. AtCPRO1, genes in corn Zm CPRO3, Zm CPRO4, genes in soybean GlCPRO2, genes in rape Bna CPRO8 are respectively introduced into rice, and then, by allowing the genes to ectopically express in oocytes, the genes are combined with MiMe body ​ Under the premise of not affecting growth and development of the plants, the genes can induce the generation of clonal diploid offspring, and provide a new solution for rice breeding, and have important application prospect and economic value.

To provide a procedure for inducing apomixis (also called agamospermy) in crops, and a method for producing an apomictic plant and an apomictic seed, and also provide a gene and a protein involved in the process of apomixis, particularly in parthenogenesis.SOLUTION: The invention provides nucleotide sequences and amino acid sequences of the parthenogenesis gene of Taraxacum as well as (functional) homologues, fragments and variants thereof, which provide parthenogenesis as a part of apomixis. Also parthenogenetic plants and methods for making these are provided, as are molecular markers and methods of using these.SELECTED DRAWING: None

The invention discloses application of a pepper miR167b gene in regulation and control of plant growth and development, and belongs to the technical field of gene engineering. An expression vector of the miR167b gene is constructed and is overexpressed in tomatoes, and the results show that the miR167b overexpressed plant is dwarfed, the fruit shape is changed, the fruit size and weight are obviously lower than those of a wild type, the fruit setting rate is high, the fruit is seedless, the number of fruit ventricles is reduced, ovules are aborted, and no embryo sac is formed in the tomato. Further analysis finds that the content of indolebutyric acid in overexpressed plant fruits is increased, which indicates that the miR167b gene can induce apomixis of fruits by regulating auxin, lays a foundation for revealing a pepper apomixis mechanism, provides gene resources and basis for improving a woody plant breeding strategy, and has important theoretical value and application prospect.

The present invention relates to nucleic acid constructs comprising the coding sequence of the apomictic trait of Eragrostis curvula ( Eragrostis curvula ) and the use of these constructs for the transformation of non-apomictic plants.

A method for producing 100% sexual cultivars of a grass plant developed from facultative apomictic germplasm. Sexual cultivars of Kentucky bluegrass include those of breeder's codes: AKB020, AKB413, AKB419, AKB484, AKB357, and AKB397. Additionally, disclosed is a method for producing hybrid apomictic plants from hybridization of a completely sexual parent with a facultative or apomictic parent, including those of breeder's codes AKB1661, AKB1658, AKB3605, AKB2839, AKB2949, AKB3128, AKB3179, AKB3201, AKB3216, AKB3241.

The present invention discloses wild type genes that upon their down regulation unequivocally induce AES formation in sexual plants. Photomicrographs of apospory in the down regulated germplasm meet all four criteria. The identified genes and methods may be used as part of a genetic engineering strategy to convert sexual crops to apomixis, which will reduce costs of hybrid seed production for crops currently grown as hybrids and will allow crops currently grown as inbred varieties, due to prohibitively high hybrid seed production costs, to be grown as superior yielding hybrids. The disclosure concerns methods of inducing unreduced aposporous or diplosporous embryo sac formation in a sexual angiosperm comprising modifying the expression level of one or more genes.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsMPF gene. The method comprises the following steps: firstly, constructing an OsMPF gene expression box driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsMPF positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsMPF gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through apomixis.

Methods and compositions disclosed herein relate to genes involved in plant production and methods of using the same.

The invention provides a specific InDel molecular marker of a key gene OsSPO11-1 for rice apomixis control and application of the specific InDel molecular marker, and belongs to the technical field of crop molecular heredity. The invention provides a 9bp deletion mutant of a second exon of a rice fertility control gene OsSPO11-1 at the 162-170bp position in a CDS (Coding Sequence) sequence. The mutation causes the deletion of three key amino acids His-Arg-Tyr in the amino acid coding sequences from the 54th site to the 56th site. In fertility expression, the homozygous type of the mutant shows the characteristic of complete sterility, and the heterozygous type of the mutant keeps complete fertility. In order to accurately identify the mutation, the invention specifically develops a specific molecular marker, as shown in SEQ ID NO.7. The invention also provides a method for identifying the mutation. Through single PCR amplification, the marker can accurately distinguish a wild type, a heterozygous type and a mutant type, and the detection result is completely consistent with the results of sequencing analysis and phenotype observation.

The present invention belongs to the fields of plant breeding and biotechnology. Disclosed is a method for inducing apomixis in plants. Specifically, the present invention relates to the use of the WUS gene in the induction of apomixis. It is found in the research of the present inventors that the MOC3 gene (renamed OsWUS) in rice can be used for constructing apomictic lines. In these lines, the seed-setting rate and the induction rate of clonal seeds show different degrees of changes. In particular, some lines maintain a normal seed-setting rate, and achieve a clonal seed efficiency of up to 22%. Therefore, the present invention has relatively good application values.

The present invention provides the nucleotide and amino acid sequences of parthenogenesis genes in the genus Taraxacum and their (functional) homologues, fragments and variants thereof, which provide parthenogenesis as part of apomixis. Also provided are parthenogenetic plants and methods for preparing them, as well as molecular markers and methods for using them.

The invention relates to the technical field of plant genetic engineering, and discloses application of a GmOSD1b protein and a coding gene thereof in regulation and control of plant meiosis. According to the invention, the GmOSD1b gene is cloned in soybean, and the gene participates in the second meiosis process and affects the normal development of gametophytes. The GmOSD1b gene is subjected to targeted editing through the CRISPR / Cas9 technology, gene resources and theoretical support are provided for heterosis fixation of soybeans through apomixis, and the GmOSD1b gene has important application value in the aspects of promoting the soybean apomixis technology and cultivating new soybean varieties.

A method for producing 100% sexual cultivars of a grass plant developed from facultative apomictic germplasm. Sexual cultivars of Kentucky bluegrass include those of breeder's codes: AKB020, AKB413, AKB419, AKB484, AKB357, and AKB397. Additionally, disclosed is a method for producing hybrid apomictic plants from hybridization of a completely sexual parent with a facultative or apomictic parent, including those of breeder's codes AKB1661, AKB1658, AKB3605, AKB2839, AKB2949, AKB3128, AKB3179, AKB3201, AKB3216, AKB3241.

The invention discloses a character-stable Chinese holly propagation method based on an apomixis phenomenon, and relates to the technical field of Chinese holly breeding and propagation. According to the method, fine flower thinning is carried out on screened female ilex cornuta plants with excellent characters before flowering of the female ilex cornuta plants; bagging and isolating immediately after flower thinning so as to completely eradicate external pollen pollution; after fruit setting, the bagging type is changed, and a protective agent is sprayed, so that physiological fruit falling is prevented; and finally, harvesting seeds and sowing to obtain a clonal offspring with the genetic character highly consistent with that of the female parent. According to the method, bagging isolation and physiological regulation are combined, the apomixis characteristic of the ilex cornuta is effectively utilized, and breeding with stable characters of excellent single plants is achieved. The method has the remarkable advantages of simplicity and convenience in operation, low cost, high propagation efficiency and stable progeny characters, and is suitable for new Chinese holly variety breeding, rapid propagation and new plant variety protection.

The invention provides a novel method for realizing apomixis of plants. By removing egg cell expression inhibition factors of endogenous parthenogenesis genes (including BBM1, BBM4, CPRO1, CPRO2, WUS and the like) of rice and combining with a MiMe strategy, a novel apomixis method which only depends on gene editing is realized. According to the method, a gene editing means is adopted completely, an egg cell ectopic expression technology is not involved, apomixis is achieved by removing an egg cell expression inhibition factor of a rice endogenous parthenogenesis gene, the purpose of not involved transgenosis is achieved, and production and application of an artificial apomixis technology in crops are achieved.

The application provides a binary expression vector for fixing soybean hybrid advantage and a construction method and application thereof, and belongs to the field of plant breeding and biotechnology. The binary expression vector is obtained by one-time construction of a CRISPR / Cas9-mediated soybean MiMe system and a ToPAR gene, and is specifically started by a soybean oocyte-specific promoter pGmEC1.1. The vector is delivered to a soybean hybrid by using an agrobacterium-mediated soybean cotyledon node genetic transformation system, and a material with full mutation of five genes of the MiMe and specific expression of the ToPAR gene is screened, so that apomixis is successfully induced in the dicotyledonous crop soybean, the pairing efficiency of hybrid soybean is improved, and the seed production risk of hybrid soybean is eliminated.

The present invention belongs to the fields of biotechnology and plant breeding. Specifically disclosed are a gene having parthenogenesis-inducing ability in plants, and the use thereof in plant breeding such as induction of haploid formation and apomixis. By means of regulating the expression of a gene that encodes a protein having parthenogenesis-inducing ability, the transition of egg cells into the embryonic development fate is effectively realized, thereby inducing parthenogenesis. The gene and a corresponding induction method therefor can be applied to related plant breeding processes such as the production of haploids and synthetic apomixis.

In contrast to meiosis, where meiotic recombination, random segregation of chromosomes and ploidy reduction occurs, Mitosis instead of Meiosis (MiMe), an engineered form of apomeiosis, leads to unreduced clonal gametes in the Solanaceae (night-shades), and, via selfing, to tetraploid offspring that stably inherit genome-wide heterozygosity and plant phenotypes. In the MiMe system, factors involved in meiotic recombination, sister chromatid cohesion, and meiosis II entry are knocked out or knocked down. Engineered clonal unreduced gamete formation by MiMe can be used as a component of synthetic apomixis, and to develop polyploid genome design.