17 results about "Organ development" patented technology

The invention relates to the technical field of plant genetic engineering, in particular to a transcription factor CsKNAT1 and application thereof in regulation and control of development of tea trees. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the sequence of protein coded by the gene is as shown in SEQ ID NO: 2. Research finds that overexpression of the CsKNAT1 gene can influence the form of plant leaves: the edges of the leaves are promoted to have notches (asymmetric leaves are formed, resulting in increase of sawteeth at the edges of the leaves); the formation of compound leaves can be promoted; leaves on plant stems can grow in a rosette form, and secondary stems are bolted in the middles of rosette leaves; the research also finds that the overexpression of the CsKNAT1 gene can obviously influence the flowering phase and the flower form of the plant; the delay of the flowering phase is promoted; overexpression of the CsKNAT1 gene can further influence development of floral organs and formation of siliques, part of arabidopsis plants have phenotypes that corolla becomes small, stamens are exposed, petals gradually extend and are zigzag in a wavy shape, the length of mature siliques is remarkably shortened by 20%-36.1%, and the phenomenon that the fruits are aborted occurs.

The invention relates to a plant floral organ dominant expression gene JAZ4 as well as an expression product, an expression vector and application thereof, and aims to solve the technical problem that the development of plant flower color and stigma mastoid cells is difficult to regulate and control at present. A gene JAZ4 capable of regulating and controlling the development of plant floral organs is cloned from tobacco, and by constructing a knockout strain and an overexpression strain of the JAZ4 gene, the flower color becomes shallow after the JAZ4 gene is knocked out, lactiform protrusions of corolla conical epidermis cells become large, the density is obviously increased, and stigma hair is increased; after overexpression, the flower color is deepened, the milky protrusion of the corolla conical epidermis cells almost disappears, the density is obviously reduced, and the stigma hair is deleted, which indicates that the NtJAZ gene can regulate and control the development of the flower color, the corolla conical epidermis cells and the stigma hair; the method is of great significance to research of a molecular regulatory network and molecular assisted breeding of plant flower color and stigma mastoid cell development.

The invention discloses a white flower ornithogalum caudatum OtEXO gene, an encoding protein and application, and belongs to the technical field of molecular biology. The nucleotide sequence of the ornithogalum caudatum OtEXO gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the ornithogalum caudatum OtEXO gene is as shown in SEQ ID NO. 2. The ornithogalum caudatum OtEXO gene can be applied to regulation and control of plant development, and overexpression of the OtEXO gene promotes leaf enlargement, accelerates plant growth and development and promotes growth of bulbils on leaves. The ornithogalum caudatum OtEXO gene disclosed by the invention provides a new gene for cultivating a new variety with enlarged leaves and other organs, excellent plant growth vigor and accelerated breeding speed, and can be widely applied to the fields of plant organ development and molecular breeding.

The invention relates to a method for improving peony double petal flower types, which belongs to the peony flower type regulation and control technology, indoleacetic acid and a Tween-20 stock solution are used for preparing auxin treatment liquid, the concentration of the indoleacetic acid in the auxin treatment liquid is 25-35 mg.L <-1 >, and the volume concentration of the Tween-20 is 0.04-0.05%; when the flower buds of the peony plants grow to the initial stage of the round peach period, spraying auxin treatment liquid to the whole peony plants by using a pressurized sprinkling can, and performing normal maintenance after spraying; when the flower buds of the peony plants develop to the initial stage of the color exposure period, spraying the auxin treatment liquid to the whole peony plants by using the pressurized sprinkling can again, and maintaining until the peony plants bloom after the auxin treatment liquid is sprayed. By means of the adjusting effect of indoleacetic acid on flower organ development, the growth hormone treatment liquid is quantitatively sprayed in the round peach stage and the initial stage of the color exposure stage, intervention on the petal forming process is achieved on the premise that plant growth and the flowering phase process are not affected, the ornamental characteristic of peonies is effectively enhanced, and the flower quality is improved. The application range of the high-double-petal ornamental variety is expanded, and the commodity value is improved.

This invention belongs to the field of plant genetic engineering technology, specifically disclosing a lotus embryo-specific promoter pNnCYP80A and its applications. The nucleotide sequence of the promoter is shown in SEQ ID NO.1. The pNnCYP80A promoter provided by this invention has strong activity and can drive the efficient and specific expression of the GUS reporter gene in the immature embryos of transgenic Arabidopsis thaliana. This promoter can drive the efficient and specific expression of exogenous genes in plant embryo organs, and has broad application prospects in the study of plant embryo organ development and the targeted improvement of embryo quality and yield traits through transgenics.

The invention relates to the technical field of bioengineering, and provides a chrysanthemum G protein coupled receptor GCR or related factors thereof and application thereof in flower development regulation. Specifically, the invention provides a method for regulating chrysanthemum flower development, the method is carried out by regulating expression of CmGCR and / or CmGCR related factors, and the CmGCR related factors are downstream factors regulated by CmGCR in a photoperiod pathway, downstream factors regulated by CmGCR in a hormone signal pathway or downstream factors regulated by CmGCR in floral organ development. The invention also provides an application of the CmGCR and / or the CmGCR related factor in regulating and controlling the flowering time of the plant. New functions of the CmGCR and the CmGCR related factors are applied to flower development regulation and control, and the inflorescence development process and the flowering time can be accurately regulated, so that the CmGCR is particularly suitable for improving germplasm in the flowering phase and creating early-flowering or late-flowering germplasm.

The present application relates to a nutritional composition and use thereof. Specifically, the present application relates to a nutritional composition comprising: (1) component A consisting of lactoferrin and nucleotides; (2) component B consisting of medium-long chain fatty acid esters and 1,3-dioleic acid-2-palmitic acid triglyceride; and (3) component C consisting of galactooligosaccharides and fructooligosaccharides. The present application also relates to the use of the nutritional composition for one or more of the following non-therapeutic purposes: promoting immune organ development; and enhancing humoral immunity. The present application also relates to the use of the nutritional composition for the non-therapeutic purpose of enhancing immunity, and the use of the nutritional composition in the preparation of a product for enhancing immunity. The component A, component B and component C of the nutritional composition have a synergistic effect in enhancing immunity, and have a good application prospect.

The invention discloses a construction method and application of a multi-organ development injury animal model based on non-coding long-chain RNA (Ribonucleic Acid). According to the present invention, it is found that the expression level of LncRNA URS0000A7E830 is significantly increased compared to the normal control group in the embryonic period amoxicillin exposed zebra fish model, and the URS0000A7E830 siRNA microinjection intervention is performed on the fertilized one-cell period zebra fish embryo based on the embryonic period amoxicillin exposed zebra fish model so as to effectively improve the multi-organ differentiation damage. The model established by the invention is novel, reliable, simple and convenient, and has important significance for illuminating the occurrence mechanism of the development injury of multiple organs and determining clinical early warning and intervention targets.

The invention discloses an application of protein TaLBD30 in regulation and control of wheat flower organ development. Experiments prove that the TaLBD30-4A gene in two homologous chromosomes of a wheat genome A, the TaLBD30-4B gene in two homologous chromosomes of a wheat genome B and the TaLBD30-4D gene in two homologous chromosomes of a wheat genome D are all mutated to cause abnormal development of floral organs, which shows that pistil deletion, stamen deletion, change of pistil into a shell-like structure and change of stamen into a shell-like structure. Therefore, the wheat with abnormal floral organ development can be used as a model for researching floral organ development. The method has an important research value.

The present application discloses a momordica grosvenori SgSUP gene and its protein, gene editing target point, recombinant expression vector, application and breeding method, belongs to the field of plant genetic engineering and genetic breeding technology, the nucleotide sequence of the gene is shown as SEQ ID NO:1.The present application clones the momordica grosvenori plant type, flowering, inflorescence structure and flower fertility key gene SgSUP for the first time, reveals that it affects plant height, flowering, inflorescence branching, small flower number and flower fertility by regulating meristem cell division and flower organ development.Through gene editing or transgenic technology, new varieties of momordica grosvenori with dwarf, stringing and self-pollination can be cultivated directionally, and the planting yield and breeding efficiency are improved significantly.

The invention belongs to the technical field of plant genetic engineering, and relates to a RuMADS1 gene related to blackberry floral organ specific expression and application of the RuMADS1 gene in improvement of fruit shape and hardness. The RuMADS1 gene is successfully separated and identified, the ORF length of the RuMADS1 gene is 846 bp, and the RuMADS1 gene encodes a protein composed of 281 amino acids. The nucleotide sequence of cDNA (complementary deoxyribonucleic acid) of RuMADS1 related to floral organ development is as shown in SEQ ID NO. 1. The spatiotemporal expression analysis shows that the expression quantity of RuMADS1 in the blackberry flower and pistil is high. It is found that pistils of flowers are obviously increased in both heterologous overexpressed arabidopsis thaliana and tomatoes, and it is found that the fruit shape index of fruits is increased and the hardness is obviously improved in overexpressed tomato plants. The gene and the protein provided by the invention have wide application prospects in the aspects of changing the fruit shape, improving the appearance such as hardness and the like and improving the comprehensive characters related to the quality of fresh food.

The application discloses a Rosa chinensis organ development regulation gene, a small peptide thereof and application, and belongs to the technical field of plant genetic engineering.The application provides a kind of gene RwCLV3 for regulating the development of Rosa chinensis flower organ, and the nucleotide sequence is shown as SEQ ID NO.1;The application effectively regulates the development of Rosa chinensis and Arabidopsis thaliana flower organ by means of genetic engineering, can significantly change the size of apical meristem and the development and quantity of flower organ.The application provides a new gene resource for improving plant flower organ development and improving ornamental.

The invention belongs to the technical field of organoid, and particularly relates to a core-shell structure vascularized bone organoid and a preparation method and application thereof. The MSCs and the ECs are cultured by adopting an organoid culture mode, the cell spheroid with a'core-shell 'structure is constructed by using the MSCs / ECs with a specific cell proportion, a microenvironment for in-vivo growth of cells can be better simulated, cell-cell connection and a complete extracellular matrix are preserved, the cell spheroid has a structure and a function similar to those of source cells or directionally differentiated stem cells, and the cell spheroid has a good application prospect. And the development process of organs is simulated, so that higher self-renewal and proliferation capabilities are achieved, the metabolic capability of cells is improved, the cell viability is improved, and good proliferation, osteogenesis and vascularization effects are achieved. The vascularized bone organ prepared by the method can be widely applied to preparation of bone repair materials.

The invention relates to an animal model for endogenous Col1a1 + cell lineage tracing and a construction method and application thereof.The construction method comprises the steps that firstly, a gene with the genotype of Col1a1-CreERT2-P2A is constructed and knocked into a mouse, the mouse is subjected to mating breeding with a fluorescent report mouse (such as CAA-LoxP-ZsGreen-Stop-LoxP-tdTomato), and a double-genotype mouse is obtained; the mouse model is used for detecting the expression condition of Col1a1 in tissues such as skull, cranial suture, joint and spine for the first time, and the fate of a cell lineage expressed in real time by using tdTomato fluorescent protein to mark endogenous Col1a1 in the processes of tissue and organ development, regeneration and repair and the like can be realized.

The application provides application of a c-kit gene and an overexpression vector thereof in preparation of a medicine for preventing and treating tooth-derived organs, including enamel development retardation / morphological deformity, and a nucleotide sequence of the c-kit gene is shown as SEQ ID No. 1. Compared with the prior art, the application has the following advantages: the application provides application of the c-kit gene and the overexpression vector thereof in preparation of the medicine for preventing and treating tooth-derived organ development retardation / morphological deformity, and a previously unidentified c-Kit-PI3K-Creb3l2 signal axis is recorded, which is crucial for normal tooth development; the path maintains the progenitor cell characteristics and proliferation capacity of dental epithelial cells, and ensures normal growth and pattern; the technology promotes the understanding of a tooth formation signal network, and is helpful for a wider field of regenerative dentistry, wherein manipulation of key signal pathways such as c-Kit and PI3K can provide information for tooth bioengineering or repair strategies.

Cranial placodes are embryonic structures essential for sensory and endocrine organ development. The efficient derivation of cranial placodes from human pluripotent stem cells is disclosed where the timed removal of the BMP inhibitor Noggin, a component of the dual-SMAD inhibition strategy of neural induction, triggers placode induction at the expense of CNS fates. Further fate specification at the pre-placode stage enables the selective generation of placode-derived trigeminal ganglia capable of in vivo engraftment, mature lens fibers and anterior pituitary hormone-producing cells that upon transplantation produce hormones including, but not limited to, human growth hormone and adrenocortiocotropic hormone in vivo. Alternatively, anterior pituitary hormone-producing cells are generated in cell culture systems in vitro.

The invention belongs to the technical field of molecular biology, and particularly relates to a polianthes tuberose PtNPY2 gene and a protein, an expression vector, a biological material and application thereof. Aiming at the problem that research on gene sequence information and functional genes of polianthes tuberosa is blank, leaf and flower organs of polianthes tuberosa are subjected to transcriptome analysis, an NPY2 gene is separated and is named as a PtNPY2 gene through amino acid conserved domain comparison, and a nucleotide sequence of the PtNPY2 gene is shown as SEQ ID NO.1. Experiments find that the expression quantity of the gene is remarkably improved in the organ formation process, then a plant expression vector is constructed and transferred into tobacco for functional analysis, and it is found that the gene can change the tobacco leaf development process, change the leaf shape and thicken leaf veins, so that the leaf shape is peculiar, and the tobacco quality is improved. The gene is a high-value target gene for regulating and controlling organ development of tuberose plants and carrying out genetic improvement. The important reference is provided for deeply researching the molecular mechanism of plant organ development and cultivating new varieties with peculiar leaf shapes.