50 results about "Organ development" patented technology

The invention discloses a spatial transcriptome data analysis method based on artificial intelligence, and belongs to the technical field of spatial transcriptomics data analysis. Firstly, self-adaptive normalization and hypervariant gene screening preprocessing are carried out on original gene expression data; then constructing a hierarchical map integrating spatial proximity and transcription similarity, and ensuring the connectivity and robustness of the map through a dynamic radius pruning and neighborhood inheritance strategy; dividing positive and negative sample sets based on the atlas, and inputting a type modulation contrast graph auto-encoder for training; and finally, spatial domain identification and downstream function analysis are completed based on the low-dimensional potential representation or reconstructed gene expression matrix output by the model. The method effectively improves the accuracy and stability of spatial domain recognition, adapts to multi-technology-source data, enhances the biological interpretability of model output, and can be widely applied to biomedical scenes such as tumor microenvironment analysis and organ development research.

The invention relates to the technical field of plant genetic engineering, in particular to a transcription factor CsKNAT1 and application thereof in regulation and control of development of tea trees. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the sequence of protein coded by the gene is as shown in SEQ ID NO: 2. Research finds that overexpression of the CsKNAT1 gene can influence the form of plant leaves: the edges of the leaves are promoted to have notches (asymmetric leaves are formed, resulting in increase of sawteeth at the edges of the leaves); the formation of compound leaves can be promoted; leaves on plant stems can grow in a rosette form, and secondary stems are bolted in the middles of rosette leaves; the research also finds that the overexpression of the CsKNAT1 gene can obviously influence the flowering phase and the flower form of the plant; the delay of the flowering phase is promoted; overexpression of the CsKNAT1 gene can further influence development of floral organs and formation of siliques, part of arabidopsis plants have phenotypes that corolla becomes small, stamens are exposed, petals gradually extend and are zigzag in a wavy shape, the length of mature siliques is remarkably shortened by 20%-36.1%, and the phenomenon that the fruits are aborted occurs.

The present invention relates to the field of biotechnology and relates to a kind of alfalfa MsCAL Gene, and its application, MsCAL The nucleotide sequence of the gene is shown in SEQ ID No.29. MsCAL The gene is specifically expressed in the plant stem apex, with the highest expression level in the floral organs during the reproductive growth stage, indicating that it is specifically involved in the plant flowering process. MsCAL Gene overexpression caused transgenic Arabidopsis to flower early, become dwarfed, and have fewer rosette leaves. In addition, the transgenic Arabidopsis changed from an indeterminate inflorescence to a finite inflorescence. Quantitative results of known genes regulating flowering time and floral organ development in Arabidopsis showed that MsCAL It can regulate the expression of downstream flowering and flower organ development related genes. MsCAL The expression delayed flowering, reduced crude fiber and lignin content, and increased forage digestibility and crude protein content.

The present invention discloses a gene module N-miR172- BraNPY5 and its application, the precursor sequence of cabbage N-miR172 is shown in SEQ ID No.1, and its target gene BraNPY5 The sequence is shown in SEQ ID No. 2. The miRNA overexpression vector and the STTM inhibition expression vector were transformed into Col-type Arabidopsis by Agrobacterium floral transformation to obtain heterologous expression Arabidopsis strains. The results showed that the inhibition of N-miR172 expression led to delayed development of young leaves, delayed flowering, and decreased pollen vitality in Arabidopsis, while 3-fold overexpression of N-miR172 led to poor development of Arabidopsis, short plants, and decreased seed germination vitality. This shows that cabbage N-miR172 plays an important role in the development of floral organs and the transition from vegetative growth to reproductive growth, and this gene module can be applied to the breeding of cabbage and other horticultural plants.

The present application relates to a method for inducing liver oval cell line to form functional liver-like organ tissue on a liver acellular biological scaffold, belonging to the field of biotechnology, wherein the liver oval cell line (HOCL) is used as seed stem cells, and is implanted into a regenerative liver acellular biological scaffold (R-DLS) with biological activity; according to the in-vitro simulation of the development of a body-like liver organ microenvironment concept, a conditional culture solution capable of inducing HOCL liver function differentiation and reconstructing a functional liver organ structure is added, and a functional liver-like organ tissue is constructed in an in-vitro 3D culture system. In the method, the HOCL in the R-DLS shows its differentiation into functional hepatocytes in 2-24h in the in-vitro 3D culture system, and the liver-like organ tissue can be formed in 21 days. The "new liver" constructed by the method has important clinical conversion value for being used as a liver transplantation donor and treating ESLD.

The invention relates to a plant floral organ dominant expression gene JAZ4 as well as an expression product, an expression vector and application thereof, and aims to solve the technical problem that the development of plant flower color and stigma mastoid cells is difficult to regulate and control at present. A gene JAZ4 capable of regulating and controlling the development of plant floral organs is cloned from tobacco, and by constructing a knockout strain and an overexpression strain of the JAZ4 gene, the flower color becomes shallow after the JAZ4 gene is knocked out, lactiform protrusions of corolla conical epidermis cells become large, the density is obviously increased, and stigma hair is increased; after overexpression, the flower color is deepened, the milky protrusion of the corolla conical epidermis cells almost disappears, the density is obviously reduced, and the stigma hair is deleted, which indicates that the NtJAZ gene can regulate and control the development of the flower color, the corolla conical epidermis cells and the stigma hair; the method is of great significance to research of a molecular regulatory network and molecular assisted breeding of plant flower color and stigma mastoid cell development.

The invention discloses a white flower ornithogalum caudatum OtEXO gene, an encoding protein and application, and belongs to the technical field of molecular biology. The nucleotide sequence of the ornithogalum caudatum OtEXO gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the ornithogalum caudatum OtEXO gene is as shown in SEQ ID NO. 2. The ornithogalum caudatum OtEXO gene can be applied to regulation and control of plant development, and overexpression of the OtEXO gene promotes leaf enlargement, accelerates plant growth and development and promotes growth of bulbils on leaves. The ornithogalum caudatum OtEXO gene disclosed by the invention provides a new gene for cultivating a new variety with enlarged leaves and other organs, excellent plant growth vigor and accelerated breeding speed, and can be widely applied to the fields of plant organ development and molecular breeding.

The invention relates to a method for improving peony double petal flower types, which belongs to the peony flower type regulation and control technology, indoleacetic acid and a Tween-20 stock solution are used for preparing auxin treatment liquid, the concentration of the indoleacetic acid in the auxin treatment liquid is 25-35 mg.L <-1 >, and the volume concentration of the Tween-20 is 0.04-0.05%; when the flower buds of the peony plants grow to the initial stage of the round peach period, spraying auxin treatment liquid to the whole peony plants by using a pressurized sprinkling can, and performing normal maintenance after spraying; when the flower buds of the peony plants develop to the initial stage of the color exposure period, spraying the auxin treatment liquid to the whole peony plants by using the pressurized sprinkling can again, and maintaining until the peony plants bloom after the auxin treatment liquid is sprayed. By means of the adjusting effect of indoleacetic acid on flower organ development, the growth hormone treatment liquid is quantitatively sprayed in the round peach stage and the initial stage of the color exposure stage, intervention on the petal forming process is achieved on the premise that plant growth and the flowering phase process are not affected, the ornamental characteristic of peonies is effectively enhanced, and the flower quality is improved. The application range of the high-double-petal ornamental variety is expanded, and the commodity value is improved.

This invention belongs to the field of plant genetic engineering technology, specifically disclosing a lotus embryo-specific promoter pNnCYP80A and its applications. The nucleotide sequence of the promoter is shown in SEQ ID NO.1. The pNnCYP80A promoter provided by this invention has strong activity and can drive the efficient and specific expression of the GUS reporter gene in the immature embryos of transgenic Arabidopsis thaliana. This promoter can drive the efficient and specific expression of exogenous genes in plant embryo organs, and has broad application prospects in the study of plant embryo organ development and the targeted improvement of embryo quality and yield traits through transgenics.

The invention relates to the technical field of bioengineering, and provides a chrysanthemum G protein coupled receptor GCR or related factors thereof and application thereof in flower development regulation. Specifically, the invention provides a method for regulating chrysanthemum flower development, the method is carried out by regulating expression of CmGCR and / or CmGCR related factors, and the CmGCR related factors are downstream factors regulated by CmGCR in a photoperiod pathway, downstream factors regulated by CmGCR in a hormone signal pathway or downstream factors regulated by CmGCR in floral organ development. The invention also provides an application of the CmGCR and / or the CmGCR related factor in regulating and controlling the flowering time of the plant. New functions of the CmGCR and the CmGCR related factors are applied to flower development regulation and control, and the inflorescence development process and the flowering time can be accurately regulated, so that the CmGCR is particularly suitable for improving germplasm in the flowering phase and creating early-flowering or late-flowering germplasm.

Disclosed are methods of accelerating development of new hair follicles in pluripotent stem cell-derived skin organoids. In particular, disclosed are methods of accelerating development of new hair follicles in skin organoids by adding one or more growth factors, such as FGF10, FGF20, BIO, ruxolitinib, tofacitinib, LDN193189, vitamin E, GDNF, retinoic acid, or IGF2. The growth factors are added to a culture of skin organoid at critical windows of time of the skin organoid development, such as after (or upon) the formation of epidermis and dermis in the skin organoid.

The invention belongs to the technical field of preparation of organoid, and particularly relates to a vessel organoid with lymphatic vessels and a construction method of the vessel organoid. According to the construction method provided by the invention, the lymphatic vessel structure formed by the lymphatic endothelial cells is successfully prepared for the first time. According to the organ with lymphatic vessels constructed by the invention, an obvious network-shaped vascular structure is formed in the functional lymphatic vessel organ; and different acellular matrixes or artificially synthesized hydrogel can be used for culturing functional vessel organs. The constructed perfect vessel organ with the lymphatic vessel and vascular structure has very important significance in exploration of organ development, drug screening, pathogenesis and tissue regeneration, and has a huge application prospect.

The present invention belongs to the field of plant biotechnology, and specifically relates to a method for establishing and applying a TRV virus-induced canna endogenous gene silencing expression system. The method comprises: CiPDS Specific fragment construction and recombination pTRV2‑PDS This invention utilizes an Agrobacterium-mediated viral infection system, uses Nicotiana benthamiana as a transitional host, and prepares viral sap. This method effectively infects canna with the TRV virus through friction inoculation. This invention establishes a VIGS technology system in canna for the first time, overcoming the limitations of traditional heterologous transformation techniques and addressing the lag in canna floral organ development research caused by the lack of efficient gene function verification tools. Furthermore, by optimizing temperature and inoculum material selection, silencing stability and phenotypic observability are significantly improved.

The invention belongs to the technical field of preparation of feed additives, and relates to a microcapsule embedding composite essential oil preparation device, a microcapsule embedding composite essential oil preparation method and essential oil, the microcapsule embedding composite essential oil preparation device provided by the invention is characterized in that a premixing stirrer, a vacuum container, an emulsification assembly, a centrifugal pump and a spray drying granulator are connected through a pipeline conveying device; through a conveying pipe and a stirring tank of the pipeline conveying device, conveying, adding and mixing of materials are achieved, and preparation of the composite essential oil is achieved. Meanwhile, the microcapsule embedded compound essential oil for the feed is prepared by compounding cinnamyl aldehyde, eugenol, thymol, carvacrol and tea tree essential oil according to a certain proportion, the problem that single essential oil is single in effect is solved, and the microcapsule embedded compound essential oil has the effects of resisting oxidation, reducing stress and promoting reproductive organ development of laying ducks, so that the laying rate is increased, and the egg quality is improved.

The invention relates to the technical field of plant genetic engineering, and particularly discloses application of a GhCOL2 gene in regulation and control of cotton flower primordium differentiation. The coding sequence of the GhCOL2 gene is as shown in SEQ ID No.1 (sequence identifier number 1). According to the invention, the GhCOL2 gene is knocked out by using sgRNA target sequences as shown in SEQ ID No.2 and SEQ ID No.3 through a CRISPR / Cas9 gene editing technology, so that the quantity of the cotton flower primordia can be effectively increased. The invention provides new gene resources and technical means for cotton flower organ development regulation and breeding, and has important agricultural application value.

The present application relates to a nutritional composition and use thereof. Specifically, the present application relates to a nutritional composition comprising: (1) component A consisting of lactoferrin and nucleotides; (2) component B consisting of medium-long chain fatty acid esters and 1,3-dioleic acid-2-palmitic acid triglyceride; and (3) component C consisting of galactooligosaccharides and fructooligosaccharides. The present application also relates to the use of the nutritional composition for one or more of the following non-therapeutic purposes: promoting immune organ development; and enhancing humoral immunity. The present application also relates to the use of the nutritional composition for the non-therapeutic purpose of enhancing immunity, and the use of the nutritional composition in the preparation of a product for enhancing immunity. The component A, component B and component C of the nutritional composition have a synergistic effect in enhancing immunity, and have a good application prospect.

The present invention relates to the technical field of genetically engineered mouse models, and specifically to a Kdf1 gene conditional knockout mouse model and a construction method thereof. The construction method comprises the following steps performed in sequence: obtaining a donor vector; obtaining gRNA; obtaining a conditional knockout mouse model capable of accurately knocking out the Kdf1 gene. The present technical solution solves the technical problem that it is difficult to accurately study the mechanism of Kdf1 regulating organ development using the Kdf1 gene functional defect mouse model of the prior art. The mouse model of the present solution can be mated and propagated with Cre mice from a variety of tissue sources, thereby constructing a mouse model of Kdf1 gene knockout with multiple tissue specificities that can be stably propagated, thereby avoiding embryonic lethality caused by systemic organ dysfunction in mice, and at the same time, the Kdf1 gene of the tooth germ epithelial tissue can be specifically knocked out, creating conditions for studying the function of the Kdf1 gene.

The invention discloses a construction method and application of a multi-organ development injury animal model based on non-coding long-chain RNA (Ribonucleic Acid). According to the present invention, it is found that the expression level of LncRNA URS0000A7E830 is significantly increased compared to the normal control group in the embryonic period amoxicillin exposed zebra fish model, and the URS0000A7E830 siRNA microinjection intervention is performed on the fertilized one-cell period zebra fish embryo based on the embryonic period amoxicillin exposed zebra fish model so as to effectively improve the multi-organ differentiation damage. The model established by the invention is novel, reliable, simple and convenient, and has important significance for illuminating the occurrence mechanism of the development injury of multiple organs and determining clinical early warning and intervention targets.

The invention relates to a method for regulating and controlling flowering time, floral organ development and seed development of rice based on microRNA172c and application, belongs to the technical field of crop genetic engineering, and can effectively regulate and control the flowering time, floral organ development and seed morphology of rice by accurately regulating the expression quantity of miR172c and a precursor gene pri-miR172c expression vector of miR172c. By increasing the expression quantity, the flowering time of the rice can be advanced, the number of glumous flowers is increased, spikelet outer hulls are increased, and seeds become slender; by reducing the expression quantity, the flowering time can be delayed, glume flower dysplasia, spikelet outer shell shortening, seed grain shape reducing and thousand grain weight reducing can be realized, and the accurate regulation provides a new means for improving rice varieties. Through the precise gene regulation and control technology, rice varieties which adapt to different environmental conditions and have specific characters can be rapidly cultivated, the yield and quality of rice are remarkably improved, and powerful support is provided for guaranteeing grain safety and promoting agricultural sustainable development.

The invention provides a method for tracing an endogenous FGFR2 < + > cell lineage fate mouse model (FGFR2-EGFP-CreERT2; the invention relates to a construction method of an endogenous FGFR2 (Fibroblast Growth Factor Receptor 2) gene, in particular to a construction method of an endogenous FGFR2 (Fibroblast Growth Factor Receptor 2) gene (ROSA26-LSL-tdTomato mouse), an EGFP-Linker-CreERT2 expression sequence is inserted through an ES (Expression Spectroscopy) targeting method, the expression of the EGFP-Linker-CreERT2 under the control of an endogenous FGFR2 promoter is realized, and an FGFR2 positive (FGFR2 +) cell is marked in real time through a fluorescent protein EGFP. Then CreERT2 expression is combined with a fluorescence report mouse (such as ROSA26-LSL-tdTomato) based on a Cre / loxP system, after fluorescent protein tdTomato expression is induced, the FGFR2 + cells can be permanently marked, and pedigree tracing of the FGFR2 + cells is defined by observing distribution of tdTomato positive cells. The mouse model provides an important tool for in-vivo research on distribution and pedigree tracing of FGFR2 + cells, and provides a new support for research on tumor generation, organ development and tissue regeneration related to FGFR2.

The invention relates to a gene RcAG2 for regulating and controlling organ development of China rose. The sequence of the gene RcAG2 is as shown in SEQ ID NO. 1. According to the invention, the RcAG2 gene is cloned in'calyx ', through heterologous overexpression of the RcAG2 gene in arabidopsis thaliana, it is found that the RcAG2 gene can significantly change the form of petals, the gene can cause gradual degradation of the petals, and the size of the petals is significantly reduced compared with a no-load arabidopsis thaliana positive strain. The result of the invention provides an important clue for further understanding the function of the RcAG2 gene in China rose development.

The present invention discloses the coding sequence of the Cymbidium flowering regulation gene CsFTL1 and its application. The Cymbidium flowering regulation protein CsFTL is characterized in that its amino acid sequence is as shown in SEQ ID NO: 2. The present invention isolated the key flower development gene FTL1 from the flower bud cDNA of the Cymbidium sinense variety 'Xiaoxiang' and named it CsFTL1. Temporal and spatial expression pattern analysis found that the expression of CsFTL1 was the highest in the flowers of Cymbidium sinense; among different flower development stages of Cymbidium sinense, the expression trend of CsFTL1 was that the mRNA accumulation increased rapidly at the stage of flower bud differentiation and then gradually decreased. After heterologously expressing CsFTL1 in Arabidopsis thaliana, it was found that Arabidopsis thaliana showed the phenotype of early flowering. The Cymbidium flowering regulation gene of the present invention can be used for the study of the molecular mechanism of flower organ development in Orchidaceae plants and the improvement of plant flowering traits.

The invention discloses an application of protein TaLBD30 in regulation and control of wheat flower organ development. Experiments prove that the TaLBD30-4A gene in two homologous chromosomes of a wheat genome A, the TaLBD30-4B gene in two homologous chromosomes of a wheat genome B and the TaLBD30-4D gene in two homologous chromosomes of a wheat genome D are all mutated to cause abnormal development of floral organs, which shows that pistil deletion, stamen deletion, change of pistil into a shell-like structure and change of stamen into a shell-like structure. Therefore, the wheat with abnormal floral organ development can be used as a model for researching floral organ development. The method has an important research value.

The present application discloses a momordica grosvenori SgSUP gene and its protein, gene editing target point, recombinant expression vector, application and breeding method, belongs to the field of plant genetic engineering and genetic breeding technology, the nucleotide sequence of the gene is shown as SEQ ID NO:1.The present application clones the momordica grosvenori plant type, flowering, inflorescence structure and flower fertility key gene SgSUP for the first time, reveals that it affects plant height, flowering, inflorescence branching, small flower number and flower fertility by regulating meristem cell division and flower organ development.Through gene editing or transgenic technology, new varieties of momordica grosvenori with dwarf, stringing and self-pollination can be cultivated directionally, and the planting yield and breeding efficiency are improved significantly.

The present invention discloses a coding sequence of a flowering regulatory gene SEP1 of Chinese orchid and its application. The present invention isolates an orchid flower organ development regulatory gene from the flower organ cDNA of the Chinese orchid variety 'Bai Mo'. After increasing its expression level, the flowering period of Arabidopsis thaliana can be significantly advanced, but the flower organ morphological structure is not affected. Through downstream gene expression analysis, it is found that the gene can positively regulate the expression of flowering control genes FT, SOC1, and LFY1, further indicating the role of the gene in promoting plant flowering. The Chinese orchid flowering regulatory gene of the present invention can be used for the study of the molecular mechanism of flowering regulation of orchidaceae plants and the improvement of flowering traits of plants.

The invention belongs to the technical field of plant genetic engineering, and relates to a RuMADS1 gene related to blackberry floral organ specific expression and application of the RuMADS1 gene in improvement of fruit shape and hardness. The RuMADS1 gene is successfully separated and identified, the ORF length of the RuMADS1 gene is 846 bp, and the RuMADS1 gene encodes a protein composed of 281 amino acids. The nucleotide sequence of cDNA (complementary deoxyribonucleic acid) of RuMADS1 related to floral organ development is as shown in SEQ ID NO. 1. The spatiotemporal expression analysis shows that the expression quantity of RuMADS1 in the blackberry flower and pistil is high. It is found that pistils of flowers are obviously increased in both heterologous overexpressed arabidopsis thaliana and tomatoes, and it is found that the fruit shape index of fruits is increased and the hardness is obviously improved in overexpressed tomato plants. The gene and the protein provided by the invention have wide application prospects in the aspects of changing the fruit shape, improving the appearance such as hardness and the like and improving the comprehensive characters related to the quality of fresh food.

The application discloses a Rosa chinensis organ development regulation gene, a small peptide thereof and application, and belongs to the technical field of plant genetic engineering.The application provides a kind of gene RwCLV3 for regulating the development of Rosa chinensis flower organ, and the nucleotide sequence is shown as SEQ ID NO.1;The application effectively regulates the development of Rosa chinensis and Arabidopsis thaliana flower organ by means of genetic engineering, can significantly change the size of apical meristem and the development and quantity of flower organ.The application provides a new gene resource for improving plant flower organ development and improving ornamental.

The invention belongs to the technical field of organoid, and particularly relates to a core-shell structure vascularized bone organoid and a preparation method and application thereof. The MSCs and the ECs are cultured by adopting an organoid culture mode, the cell spheroid with a'core-shell 'structure is constructed by using the MSCs / ECs with a specific cell proportion, a microenvironment for in-vivo growth of cells can be better simulated, cell-cell connection and a complete extracellular matrix are preserved, the cell spheroid has a structure and a function similar to those of source cells or directionally differentiated stem cells, and the cell spheroid has a good application prospect. And the development process of organs is simulated, so that higher self-renewal and proliferation capabilities are achieved, the metabolic capability of cells is improved, the cell viability is improved, and good proliferation, osteogenesis and vascularization effects are achieved. The vascularized bone organ prepared by the method can be widely applied to preparation of bone repair materials.

The invention relates to an animal model for endogenous Col1a1 + cell lineage tracing and a construction method and application thereof.The construction method comprises the steps that firstly, a gene with the genotype of Col1a1-CreERT2-P2A is constructed and knocked into a mouse, the mouse is subjected to mating breeding with a fluorescent report mouse (such as CAA-LoxP-ZsGreen-Stop-LoxP-tdTomato), and a double-genotype mouse is obtained; the mouse model is used for detecting the expression condition of Col1a1 in tissues such as skull, cranial suture, joint and spine for the first time, and the fate of a cell lineage expressed in real time by using tdTomato fluorescent protein to mark endogenous Col1a1 in the processes of tissue and organ development, regeneration and repair and the like can be realized.

Disclosed in the present invention are a spatial three-dimensional genomics sequencing method based on a microfluidic chip and the use thereof. According to the method of the present invention, the molecular labeling process of a tissue section is segmented, that is, on the basis of the process of "overall labeling — partial lysis — partial labeling — overall lysis", position labeling at the micron level is firstly performed at the spatial position level on the tissue section, then the tissue section is lysed into protein-nucleic acid complexes at the nanometer level by means of ultrasonic disruption, the macromolecular complexes are individually labeled at the nanometer level, and finally the macromolecular complexes are lysed to obtain DNA molecules containing both spatial position information and chromatin conformation information for library construction and sequencing. The method of the present invention allows for efficient joint analysis of resolution at the visualized micron level and the non-visualized base length level, and thus can be applied to the development or preparation of a product for disease detection or a product for observing organ development.