106 results about "Organ development" patented technology

The present invention is directed to a novel auxin-inducible gene, ARGOS, that is involved in organ development, including size control, in plants. Methods of influencing this development are also described, as are transformed cells and transgenic plants comprising the described sequences.

The invention aims to provide bacillus subtilis WL-04 and application thereof to livestock breeding. The preservation number of the bacillus subtilis WL-04 is CCTCC NO:M 2013343. The bacillus subtilis WL-04 can be used for remarkably inhibiting the growth of Escherichia coli, salmonella, clostridium pefringens and other conditional pathogenic bacteria of the intestinal canal of livestock, and particularly, the bacteriostasis rate of the bacillus subtilis WL-04 to Escherichia coli and clostridium perfingens is above 75%; the enzyme production is stable; the average activity of alpha-amylase is 22U / mL, and the average activity of neutral protease is 170U / mL; the reproduction of probiotics of intestinal canal can be promoted; high temperature for pelletizing can be endured; the influence of inverse environments with low-pH gastric fluid and high-concentration cholate is endured; the production performance of breeding animals can be effectively improved; immune organ development can be promoted. The bacillus subtilis WL-04 can be widely applied to livestock feeds as a feed additive.

The invention discloses a three-dimensional cell culture system, comprising container, and cell culturing medium and three- dimentioal cell culture unit inside container; said three- dimentional cell culture unit comprises implanting cell and cavity flavoable for cell adsoption, growth, differentiation and maturity. Said three- dimentional culture unit is transparent, which is flavouable for obeservation of adsorption, enlargement, transition, proliferation, differentiation, maturity, aging, death and tissue or organ development. The invention can be used for long term cell culture and large amount of cell production as seed cell for tissue engineering, and for short term regenerative tissue and micro organ formation for replanting. The invention provides special condition for tumor external research, and convenient for tumor diagnosis, observation of tumor immersion and transfer, and anti- tumor medicine sift. It can aslo be used for cell isolated culture and for research of gene programm change and influence to cell from rxternal environment.

The present invention provides a synthetic nutritional formulation comprising a substantially pure free N-acetyl glucosamine in concentrations effective for enhancing organ development, enhancing an immune response, or increasing tissue repair or healing in a subject. A synthetic nutritional formulation of the invention may optionally comprise one or both of substantially pure free sialic acid and / or substantially pure free fucose.

It is revealed that an organ such as pancreas can be regenerated by utilizing a fact that the deficiency of an organ is complemented by injecting an induced pluripotent stem cell (iPS cell) into a developed blastocyst in a blastocyst complementation method. Thus, the present invention has solved the above-described object. This provides a method for producing a target organ, using an iPS cell, in a living body of a non-human mammal having an abnormality associated with a lack of development of the target organ in a development stage, the target organ produced being derived from a different individual mammal that is an individual different from the non-human mammal.

The present invention is based in part on the discovery of significant improvements to cell culture systems and methods of generating organoids. The system of the invention provides a novel spinning bioreactor platform for higher-throughput 3D culturing of stem cells (e.g. human induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs)). The system can be widely used as a standard platform to generate stem cell-derived human organoids for any tissue and for high-throughput drug screenings, toxicity testing, and modeling normal human organ development and diseases.

The invention discloses a method for batch breeding of loach fry, which notably improves catalytic production rate of the loach. The method comprises the steps of: a, selecting loach parent for breeding to perform prenatal strengthening cultivation; b, building a third-level filtering reservoir device for spawning and hatching; c, selecting composite of the maleic acid domperidone and the trout luteinizing hormone releasing hormone analogue as a catalyst to catalyze male and female loaches, wherein per kilogram of female loaches need 10-15mg of the catalyst; per kilogram of male loaches need 5-7.5mg of the catalyst; enabling the female loaches and the male loaches to spawn and inseminate in a net cage in the jar according to the ratio of the female loaches to the male loaches being 1:2; taking out the loaches, enabling the spawns to continuously hatch; producing loach fry in a large scale; d, feeding the initial feed, putting the loaches to the pond; according to organ development characteristic of the loach fry, controlling diet conversion time point, electing palatable bait, scientifically feeding the bait, and strengthening water quality and disease control and prevention.

The invention discloses a transwell micro-fluidic chip combined with three-dimensional culture and a preparation method of the micro-fluidic chip. The micro-fluidic chip is mainly composed of a top chip layer, a porous filtration membrane, an intermediate chip layer and a bottom chip layer, wherein the porous filtration membrane seals the lower surface of the top chip layer in an irreversible manner and is adhered to the upper surface of the intermediate chip through PDMS (Polydimethylsiloxane). A pit structure of a bottom culture room is used for three-dimensional culture of in-vitro cells, tissues and organs. The transwell micro-fluidic chip disclosed by the invention simultaneously has functions of a transwell chamber, a micro-fluidic chip and in-vitro three-dimensional culture, can achieve the effect of observing cell growth conditions on the bottom chip in real time and can be applied to biological studies such as drug metabolism, organ development and the like.

The invention provides a composite nutritional type lick brick for Tibetan sheep and a preparation method thereof. The composite nutritional type lick brick for Tibetan sheep comprises the following components in percentage by weight: 14-18% of corn powder, 5-8% of wheat bran, 4-7% of rapeseed dregs, 13-17% of molasses, 7-10% of urea, 12-17% of table salt, 1-3% of calcium hydrophosphate, 1.5-2.5% of calcium carbonate, 3-6% of sodium bicarbonate, 0.5-1.5% of sodium thiosulfate, 0.65-1% of coating composite multi-vitamins, 0.35-0.5% of rumensin, 6-9.5% of bentonite, 13-16% of cement and 1% of a premix; the premix comprises the following components in percentage by weight: 5-8% of ferrous sulphate, 2.1-3.3% of copper sulphate, 9-15% of manganese sulphate, 7-11% of zinc sulphate, 0.1-0.5% of sodium selenite, 0.8-1.1% of potassium iodide, 0.4-0.6% of cobalt chloride and 61.7-74.4% of bentonite. The lick brick prepared by the preparation method provided by the invention is rich in nutrition, reasonable in hardness design, proper in feed intake, low in immersion loss rate, and capable of providing balanced nutrition for the growth and development of the Tibetan sheep, increasing the feed conversion rate and improving the reproductive performance of the Tibetan sheep, and promoting the propagative organ development of female Tibetan sheep.

The invention discloses a 4-168 laying hen nutrition set meal application model capable of promoting growth and building bodies, and belongs to the application field of feed compositions plus a feeding method. Aiming at physiological characteristics of a chick state of laying hens and through a plurality of times of demonstrations and laboratory screenings, researchers develop results that corn, fermented soybean meal, extruded soybean, soya-bean oil, glucose, compound amino acid, complex enzyme preparation, probiotics and the like serve as main raw materials, digestible amino acid is adopted for designing a nutrition level, an accurate scale of feeding is adopted, the fact that the laying hens only need to ingest effective nourishment is guaranteed so as to achieve the purpose that organ development and phylogeny are good. The laying hens can reach the standard at every yearling, when the 15 yearlings are reached, namely the replacement period comes to an end, the weight of the laying hens reaches the breed requirements, namely 1320 grams, importantly, the weight and shin length evenness both exceed 90%, and replacement pullets which are strong in physique, good in intestinal health state and strong in premunition, and a solid foundation that laying hens lay more eggs is laid.

The invention relates to a Chinese rose transcription factor RhMYB4 and application thereof to the regulation and control of floral organ development. Specifically, the invention provides an RhMYB4 gene; the gene is characterized by comprising coding region nucleotide sequences shown as 263 bp to 1039 bp of SEQ ID NO. 23. The invention further provides protein which is coded by the gene or has anamino acid sequence shown as SED ID NO. 24. The invention further provides application of the gene to the regulation of the floral organ development of plants and a low-temperature-resisting capability. Based on the function of the transcription factor RhMYB4 which is found at present, the gene can be applied to the floral organ development of the plants, such as double flower petals, heteromorphosis of the flower petals, stamen abortion and the regulation of the low-temperature-resisting capability.

The invention relates to the technical field of medical bioengineering, in particular to a monoclonal antibody resisting RNA epigenetic modification m6A, a composite used for preparing the monoclonal antibody, a hybridoma cell strain secreting the monoclonal antibody, and application of the monoclonal antibody in detecting the methylation degree of RNA in different organs and tissue, organs and tissue at different developmental stages and tumor tissue. By means of the monoclonal antibody, denatured-state m6A molecules adsorbed to a nitrocellulose membrane can be specifically recognized, and the methylation degree of RNA and distribution of m6A in an RNA transcript can be accurately and sensitively detected; the monoclonal antibody can be used for studying the regulating effect of RNA methylation modification on the physiological and pathological processes of human organ development and tumor formation, and has great significance on study of a post-transcriptional regulation mechanism of gene expression and related diseases.

The invention discloses application of a medicago truncatula MYB transcription factor MtMYB1. According to the invention, a gene MtMYB1 for regulating and controlling development, flowering phase andgrowth period of floral organs and changing a plant type is colonized from medicago truncatula flower, and the sequence of the gene MtMYB1 is SEQ ID NO.1. The analysis of mRNA (messenger Ribonucleic Acid) expression of the gene shows that the MtMYB1 is expressed in flower tissues with strong advantages; phenotypic change of arabidopsis thaliana for further over-expression of the MtMYB1 shows thatthe MtMYB1 has an important regulation and control effect on the development, the flowering phase and the growth period of the floral organs and the plant type. The invention discloses the applicationof the gene in genetic engineering of morphology, the flowering phase and the growth period of the floral organs as well as plant type transformation.

The invention discloses a production technology of bacillus subtilis fermented soybean meal. The production technology comprises the following steps: (1) bacillus subtilis strain activation; (2) bacillus subtilis strain liquid culture; (3) bacillus subtilis strain culture in a triangular flask; and (4) soybean meal fermentation. The production technology is simple and easy to understand, by the use of bacillus subtilis, prawn diseases can be reduced, and the prawn yield can be greatly improved, so that the economic benefit and biological environmental friendliness are improved, aquatic animal immune organ development can be stimulated, immunity of the organism is enhanced, the prawn diseases can be reduced, and the prawn yield can be greatly improved, so that the economic benefit and biological environmental friendliness are improved, the water quality is purified, no pollution and residue can be produced.

The invention discloses a high-throughput plant organ development related SSR molecular marker method of the molecular biology field. According to the method provided by the invention, with the flowers of rose as the example, more than hundred thousand of SSR primers can be obtained at one time, and 3387 pairs of the SSR primers are related to functional genes expressed in a rose development process. A lot of SSR molecular markers are selected, have polymorphism, are suitable for different application selections, can well provide genetic background for analysis and excavation of related genes, understand the domestication history of Chinese old rose, cultivation domestication of rose, and rose germplasm resource diversity, and also can provide a set of SSR molecular marker system for relative relationship identification and genetic map construction of other rosaceae plants. The method provided by the invention almost completely relies on computer simulation and calculation, gets rid of the condition restriction of laboratories and the influence of uncontrollable factors, and the obtained molecular markers are large in quantity, reliable, and have good polymorphism.

The invention discloses a rice glume development gene promoter p-TRI1 and application thereof. The promoter provided by the invention comes from ordinary cultivated rice O. sativa L. 93-11 which belongs to Oryza species, and the promoter is a DNA molecule of the following 1), 2) or 3): 1) a DNA molecule shown in Sequence 1 in a sequence table; 2) a DNA molecule which crosses with a DNA sequence limited by 1) or 2) under strict condition and has the promoter function; and 3) a DNA molecule which has more than 90 percent of homology with the DNA sequence limited by 1) or 2) and has the promoter function. The discovery of the promoter and the elucidation of the promoter function have important theoretical and practical significance for studying flower organ development mechanism of rice, especially the glume development mechanism and for breeding rice varieties of specific grain types. The promoter has important theoretical and practical significance for studying the molecular mechanism of rice glume development and for rice grain type molecular breeding. The promoter has wide application and market prospect in the field of agricultural.

The invention belongs to the field of molecular biology, and discloses a nymphaea floral organ development gene NsAGL6 as well as a plant expression vector and a construction method thereof. The sequence of the nymphaea floral organ development gene NsAGL6 is shown in SEQ ID No.1. The expression vector pCAMB IA 1301-220-NsAGL6 constructed in the invention is obtained by connecting the NsAGL6 gene to the linear pCAMB IA 1301-220 plasmid. The plant expression vector is applied to plant genetic transformation, the NsAGL6 gene is over-expressed under the drive of a CaMV35S starter, the AGL6 protein is synthesized in quantity, and the expression of the downstream gene is regulated so that the nymphaea blooms in advance and the plant flower type is changed.

The divisional application of the invention discloses a comb growth feed achieving comb growth consistency and increasing laying rate for laying hens of 16 weeks and with laying rate of 2%, belonging to the field of feed compositions. The feed is used for the laying hens of 16 weeks and with laying rate of 2%, and can promote the propagative organ development and weight gaining; the feed is mainly prepared from the following raw materials in parts by weight: 54.365 parts of corn, 3 parts of rice bran, 2.5 parts of wheat bran, 14 parts of bean pulp, 3 parts of fermented soybean meal, 3 parts of peanut meal, 2.8 parts of sunflower seed meal, 2 parts of rapeseed dregs, 3.5 parts of distillers dried grains with soluble (DDGS), 3 parts of corn bran, 1.0 part of soybean oil, 5.2 parts of mountain flour, 0.8 part of calcium hydrogen phosphate, 0.35 part of table salt, 0.13 part of methionine, 0.12 part of 70% lysine sulfate, 0.05 part of probiotics, 0.05 part of a complex enzyme preparation, 0.012 part of phytase, and 1 part of a compound premix. By using the feed, the comb growing consistency in a comb growth phase, large eggs in a preliminary egg laying stage, fastness in speed of reaching the egg laying peak, and the relatively high laying rate can be achieved.

The invention belongs to the field of artificial intelligence, and particularly relates to a mouse embryo organ recognition and scoring method and a system. The method comprises the following steps: collecting original images of mouse embryos in different periods, and carrying out the manual marking; inputting the mouse embryo original image and the annotation file into a Mask-RCNN network for training to obtain an organ recognition model; intercepting organs in the marked image from the original image, taking the organs and corresponding scores as training set data, and training different convolutional neural networks to obtain an image scoring model capable of scoring development of each organ; inputting a to-be-recognized mouse embryo original image into the organ recognition model, and outputting all organs in the image; intercepting all organs from the original image, and respectively inputting the organs into an image scoring model to obtain a development score of each organ; and obtaining a total score. According to the method, the organs in the mouse embryo can be quickly and accurately recognized, and the current development stage of each organ can be judged.

The invention relates to early flowering Chia (Salvia hispanica L.) strains that are suitable for culturing in a temperate area for seed production. Mutations are introduced to wild type chia seeds. Desired mutant progeny having normal appearance and an altered flora organ development is subsequently identified.

The invention discloses nutrient oil as well as preparation and application thereof, and belongs to the technical of grease processing. The nutrient oil is applicable to pregnant women and infants since 2-3.5% of DHA (Docosahexaenoic Acid) algal oil is added into plant oil; or the nutrient oil is applicable to the old since 2-3.5% of DHA algal oil and 2-3.5% of arachidonic acid oil is added into the plant oil. Materials of the nutrient oil are prepared according to formulae and are stirred and mixed at certain temperature and pressure, and thus a product, namely the nutrient oil, is prepared. For pregnant women, the nutrient oil disclosed by the invention is capable of promoting healthy development of fetuses, reducing the probability of postnatal depression, and increasing the content of DHA in breast milk. For infants and children, brain and eyesight development can be promoted, the sleep quality can be improved, the attention and the learning capability can be improved, and muscle and organ development can be promoted. For the old, angiocarpy health can be improved, grease and sugar metabolism can be regulated, the risk of senile cognitive competence and eyesight degradation can be reduced, tumor can be prevented, and postoperative rehabilitation can be promoted.

The invention relates to the technical field of poultry cultivation, in particular to a breeding method for increasing the survival rate of forcibly-weaned lambs. The breeding method comprises the following steps: forcibly weaning the lambs aged 10 days; after weaning, performing adaptation period management, internal organ development promoting management and bone development promoting management and respectively feeding different diets during management. According to the breeding method for increasing the survival rate of the forcibly-weaned lambs, provided by the invention, the lambs aged 10 days can be quickly weaned, and after weaning, the good development of various signs of the lambs can be ensured. When the breeding method provided by the invention is adopted for performing weaning management on the lambs aged 10 days, the overall incidence of various diseases during management is lower than 0.03 percent, the development indexes of internal organs are higher, the bone development is faster, and the weight increasing speed is high.

The invention provides a Paphiopedilum GLO / PIMADS-box gene sequence and a polypeptide encoded by the gene sequence. In addition, the present invention also discloses a method for obtaining the clone of P. paphiopedilum GLO / PI gene. The method is to design a degenerate primer for the amino acid conservation region of the closely related species GLO / PIMADS-box gene, extract total RNA from the flower of P. -PCR amplification, combined with RACE technology to clone to obtain the full-length cDNA sequence of the GLO / PIMADS-box gene of Paphiopediphyllum. The P. paphiopedilum GLO / PIMADS-box gene of the present invention is a new gene related to the development of P. paphiopedilum. The P. paphiopedilum GLO / PI gene provided by the present invention not only lays a foundation for further research on the function of the P. paphiopedilum GLO / PI protein, but also provides a certain basis for the molecular mechanism of the paphiopedilum organ development.

The invention belongs to the field of plant molecular biology, and particularly relates to a loquat flower organ development related transcription factor gene EjPI and application thereof. The full length of the cDNA sequence of EjPI gene is shown in SEQ ID NO. 1, and the amino acid sequence of the coded protein is shown in SEQ ID NO. 2. The EjPI gene of the invention is expressed only in petals,filaments and anthers, but not in sepals and pistils. The EjPI gene overexpression vector is transformed into wild-type arabidopsis and pi-1 mutant by agrobacterium-mediated inflorescence staining. The results show that overexpression of the EjPI gene in wild-type arabidopsis could make the sepals of arabidopsis valvulae and open; and the overexpression of the EjPI gene in the arabidopsis pi-1 mutant can restore petals and stamens. The transgenic plant material obtained by using the EjPI gene overexpression vector can enable the wild-type angiosperms to achieve sepal petalization and can be used for breeding double petal flowers; and the plant pi mutant restores petals and stamens, and a reproduction process is completed effectively. The gene can be used for plant double flower transformation so as to improve ornamental value and restore stamen organs for cross breeding, and has good application prospect.

The invention discloses a cucumber AG-like gene CsMADS24 overexpression carrier and application thereof to flower organ modification, and belongs to the technical field of biology. The carrier is a plant expression carrier containing double 35S promoters and a cucumber gene CsMADS24. A CsMADS24 transgenic plant obtained by overexpressing the CsMADS24 in Arabidopsis thaliana is in a flowering state at the early development stage of flowers; and at the later development stage of the flowers, only few relatively thin and long flowers can be formed, the petals and the stamens of most of the flowers cannot normally develop, and only sepals and carpel develop. The result shows that CsMADS24 play an important control role on the aspect of flower organ development. A novel flower organ variant material obtained by virtue of the gene CsMADS24 can be applied to breeding of crops and ornamental plants and has certain agricultural and ornamental values.

The invention provides a rubber tree HbAG gene, having a nucleotide sequence as shown in SEQ ID NO: 1. The invention further provides a protein coded with the HbAG gene and application of the HbAG gene. The HbAG gene is cloned from a rubber tree for the first time, and the gene belongs to AG subfamily, participates in development of flowers, formation of ovules and maturation of seeds and executes the functions of D genes. Subcellular localization analysis shows that the gene is localized in a cell nucleus and thus conforms to the feature of a transcription factor. The gene is further transferred to arabidopsis to obtain a transgenic plant, and the transgenic plant is discovered to have such phenotypes that the flowering time is obviously advanced, the nutritional growth time is short, and the plant is dwarfed and the like, so the gene plays an important control role in flower organ development and reproductive development of rubber trees. By using the HbAG gene for genetic improvement on rubber trees, an effective gene resource can be provided for shortening the breeding periods of the rubber trees, improving the breeding efficiency and the like.

The invention belongs to the technical field of plant gene engineering, and discloses a pleiotropic gene DDF1 (dwarf and delayed flowering 1) for controlling vegetative growth and development of floral organs of rice and application thereof, particularly mapping, cloning, function verification and application of a pleiotropic gene for controlling vegetative growth and development of floral organsof rice. The nucleotide sequence of the gene DDF1 is disclosed as SEQ ID NO.1 in the sequence table, and the amino acid sequence coded by the gene DDF1 is disclosed as SEQ ID NO.2 in the sequence table. The DDF1 determines the vegetative growth and development of floral organs of rice, especially the development of stamen. Gene engineering technology can be utilized to purposefully regulate the expression of the gene in rice, thereby regulating the plant type and fertility of rice, and creating new species of rice seeds. Therefore, the invention has wide application prospects in genetic improvement of rice.

The invention discloses a plant lodging and flower organ development regulation protein ZmLA1, and a coding gene and an application thereof. The protein ZmLA1 is from Zea mays L., and is composed of an amino acid sequence represented by a sequence 1 in a sequence table. The protein or the gene can be used for regulating at least one of the following four properties of a target plant: the auxin-to-base transport efficiency, the auxin lateral transport efficiency, the stem lodging resistance ability and the flower organ development; and the flower organ growth properties comprise at least one of pistillate flowering, tassel flowering, tassel branch number, ear length, ear handle number of filaments, ear fresh weight, cluster length and fruiting rate. The gene and the protein provided by the invention have wide application prospects in the improvement of the properties directly relating to the plant steam lodging resistance, the ideal strain, the flower organ form and the like.

A cell culture system and method of generating organoids is provided. The system includes a multiwell culture plate having shafts for mixing culture media within wells of the culture plate. The multiwell culture plate includes a base substrate having a plurality of culture wells, and a shaft operably associated with each culture well, each shaft being configured to mix media present in each culture well and having a gear adapted to operably associate with a gear on a shaft associated with an adjacent culture well. The system further includes a motor having a drive shaft in operable communication with the shaft gears of the multiwell culture plate. The system can be widely used as a standard platform to generate stem cell-derived human organoids for any tissue and for high-throughput drug screenings, toxicity testing, and modeling normal human organ development and diseases.