Production technology of bacillus subtilis fermented soybean meal
A technology of Bacillus subtilis and fermented soybean meal, which is applied in application, animal feed, animal feed, etc. It can solve the problems of uneven quality of fermented soybean meal products and no uniform standard for index evaluation, so as to reduce the occurrence of shrimp diseases and improve the digestion and utilization of animals. High rate, enhance the body's immunity
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[0017] Referring to the production process of Bacillus subtilis fermented soybean meal in this embodiment, it includes the following specific steps:
[0018] (1) Activation of Bacillus subtilis strains, A. Preparation of Bacillus subtilis culture medium: 3g beef extract, 10g peptone, NaC15g, agar 2g, water 1000ml, and prepare Bacillus subtilis strain culture with pH 7.0~7.2 Base; B. Activation of Bacillus subtilis strains: transfer the Bacillus subtilis strains to the Bacillus subtilis strain medium for activation, and cultivate at 37°C for 24 hours;
[0019] (2) Liquid culture of Bacillus subtilis strains, A. Preparation of Bacillus subtilis strain liquid culture medium: prepare 3g beef extract, 10g peptone, 15g NaC, 1000ml water, pH 7.0-7.2, sterilize at 121°C for 30 minutes; B. Cultivating Bacillus subtilis strains liquid: pick the activated Bacillus subtilis strains to inoculate 5 ml of Bacillus subtilis liquid medium, and cultivate at 37°C for 24 hours;
[0020] (3) Bacillus su...
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