The application belongs to the technical field of construction of
animal tumor models, and particularly relates to a stably passaged
immunodeficient mouse model constructed by utilizing an NK / T
lymphoma cell strain and a construction method of the model. The method comprises the following steps: pretreating an innoculation object and a
lymphoma cell strain respectively, preparing tumor suspensionfor injecting into a mouse, enabling
tumor cells to passage and the like. The
immunodeficient mouse model can be used for
drug screening or
therapeutic effect evaluation in
medical science. The construction method for constructing the
lymphoma animal model by utilizing the NK / T lymphoma
cell strain, which is provided by the application, utilizes the fixing effect of
matrigel, can be used for fixing lymphoma cells beneath
skin, so that the
tumor formation rate of the mouse is ensured, and the technical advantages of fast
tumor formation and high
tumor formation rate are manifested; on the otherhand, in the process of passage, the method cooperates with the cryopreserved operation of a relevant
tumor tissue culture solution, and the lymphoma
animal model for passage can be recovered at anymoment and directly constructed.