58results about How to "High tumor formation rate" patented technology

The invention relates to a rat liver cancer model with different liver matrix hardness backgrounds and a preparation method of the rat liver cancer model. The method for preparing the rat liver cancer model with different liver matrix backgrounds is characterized by specifically comprising the following steps of: after grouping the rats, injecting pure carbon tetrachloride to an abdominal region in a hypodermic manner; injecting a carbon tetrachloride-olive oil solution to the abdominal region in the hypodermic manner; detecting through a texture analyzer to form the rat model with different liver matrix hardness; taking liver cancer cells which are in-vitro cultured and grown well and injecting the liver cancer cells to the rat to form hypodermic liver cancer cells; in-situ planting the formed liver cancer tumor source to the rat model with different liver matrix hardness to obtain the rat liver cancer model with different liver matrix hardness backgrounds. The rat liver cancer model with different liver matrix hardness backgrounds can be used for well displaying liver cancer malignant pathological characteristics under the liver matrix hardness interference, simulating and reproducing pathological characteristics of clinical liver matrix hardening background liver cancer and solving the problems that an ideal experiment system, an animal model and the like are lacked in reaching the development of the liver cancer due to the liver matrix hardness changes.

The invention discloses a method for establishing a PDX (Patient Derived Xenograft) model of human blood tumor. The method comprises the steps of extracting a blood tumor cell of a patient, adding rabbit anti-human thymocyte immunoglobulin ATG (Anti-Thymocyte Globulin) and patient autologous serum, mixing, incubating and after completing the incubation, re-suspending the obtained cell and inoculating in a mouse; and feeding the inoculated mouse with CsA (Cyclosporine A) and lasting for 5-10 days starting from 2 days before the inoculation of the blood tumor cell. The method disclosed by the invention has the beneficial effects that a novel highly immunodeficient NCG mouse independently developed in China is firstly adopted to establish a human leukemia PDX model; by orally taking human thymocyte immunoglobulin pretreatment sample combined with the cyclosporine for a long term, donor-derived T cells are removed and inhibited; and by combining the toxic effect of the ATG on lymphocytes with the functional blocking effect of the CsA on T lymphocytes, the immune function of the T lymphocytes can be persistently inhibited and the success rate of PDX modeling of the blood tumor is significantly improved.

The invention discloses a method for shortening the period of a PDX model for xenograft of a human tumor. During the PDX model building period, spinal cord mesenchymal stem cells and matrix gel Matrigel are used for pretreating tumor tissues or tumor cell lines of a patient, and the treated tumor tissue or tumor cell of the patient is inoculated with an immunodeficient mouse (Balb/c or NOD/SCID) to manufacture a preclinical tumor model. The construction period of the solid tumor PDX model is obviously shortened, and the success rate is improved. The invention especially relates to a preparation method for a cancer mouse model with high wettability and metastasis. The method for shortening the period of the human tumor xenograft PDX model has important value for early clinical evaluation, treatment and prognosis observation of solid tumors.

The invention provides a synovial sarcoma cell line hss-005R, which is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO. C201911. The invention further provides an offspring cell line of the synovial sarcoma cell line hss-005R. A new human synovial sarcoma cell line is established, which is stable in character and can be stably passed for multiple generations. Under the premise of retaining main clinical biological characteristics, the established human synovial sarcoma cell line has the characteristics of high tumor formation rate, short incubation period, good uniformity and the like, enriches a synovial sarcoma cell bank, and can successfully establish a synovial sarcoma animal model, wherein the established animal model can be used for fundamental research and drug screening, so as to provide powerful scientific research data for the research developed on the basis of Chinese population genetic background, and provide new test material for test on sensibility and tolerance of a clinical anti-cancer drug of a new drug preclinical study in vivo experiment.

The present invention relates to the technical field of medical evaluation and detection. The invention discloses a method to prepare a breast cancer immunodeficiency mouse model, which establishes a human breast cancer cell Nod-SCID mouse model firstly and successfully at home and abroad by regular injection of estrogen preparations to the immunodeficiency mice and transplant of the breast cancer cells after thereof. The breast cancer immunodeficiency mouse model constructed by the invention has short construction period, high tumor formation rate, low animal mortality, simple and easy construction method and low cost, and thus is an ideal breast cancer immunotherapy animal model.

The invention relates to a method for cryopreserving tumor tissue. The method includes the steps that tumor tissue from a tumor patient is sheared to be in the size of 1 mm+/-0.2*1+/-0.2 mm*3+/-0.6 mm, and then the sheared tissue blocks are cryopreserved for 25+/-2 weeks. Optionally, the tissue cryopreserved for 25+/-2 weeks are used for building a PDTT transplantation tumor model or in-vivo or in-vitro serial subcultivation. The cryopreserving method has the advantages that the tumor formation rate is high, and the form and the molecular characteristic of source tissue of the patient can be completely reserved through transplantation tumor.

The invention relates to the field of drug efficacy screening, and particularly discloses a tumor in-vitro culture method. The tumor in-vitro culture method includes the steps that tumor tissue samples are collected; the tumor tissue samples are treated, and single tumor cells and macrophages are sorted; the single tumor cells are placed in an environment full of hydrogel and then are screened toobtain tumor stem cells; the tumor stem cells and the macrophages are adopted to build a co-culture model to obtain in-vitro culture tumor samples. In this way, the tumor stem cells with high proliferation and differentiation capabilities can be screened out in vitro, therefore, the tumor stem cells are implanted into the animal body, the tumor formation time can be greatly shortened, the tumor formation rate is increased, and then susceptibility testing is conducted; therefore, the progress of susceptibility testing is speeded up, and time for treating patients is bought.

The invention provides a method building a mice portable breast cancer tumor cell suspension orthotopic model. The method building the portable breast cancer tumor cell suspension orthotopic model comprises the following steps that a mice spontaneous breast cancer tumor is grinded in a mesh screen, centrifugation is conducted, tumor cell suspension with cell contraction of (0.7-1.9) * 106 cell/mL is manufactured by nutrient solution in a deployed mode, 0.2 to 0.4 mL of the tumor cell suspension is injected under the front limb armpit skin of each mouse, the mice are bred normally for 9 to 21 days, and the construction of the mice portable breast cancer tumor cell suspension orthotopic model is finished. An injected tumor cell is formed by real C3H mice tumor cells inside the body of the mouse by grinding, the growing process of the breast cancer cell inside the mouse body is simulated, and the mice are bred normally without a particular environment after the injection is finished. After transplantation, lesions are found to be formed by the C3H at the front limb armpit of the mice are found through detection, the method building the mice portable breast cancer tumor cell suspension has the advantages of being high in occurrence rate, short in time, high in tumor formation rate, low in cost and the like, and therefore the method building the mice portable breast cancer tumor cell suspension can be widely applied to experiments of breast cancer treatment.

The application belongs to the technical field of construction of animal tumor models, and particularly relates to a stably passaged immunodeficient mouse model constructed by utilizing an NK/T lymphoma cell strain and a construction method of the model. The method comprises the following steps: pretreating an innoculation object and a lymphoma cell strain respectively, preparing tumor suspensionfor injecting into a mouse, enabling tumor cells to passage and the like. The immunodeficient mouse model can be used for drug screening or therapeutic effect evaluation in medical science. The construction method for constructing the lymphoma animal model by utilizing the NK/T lymphoma cell strain, which is provided by the application, utilizes the fixing effect of matrigel, can be used for fixing lymphoma cells beneath skin, so that the tumor formation rate of the mouse is ensured, and the technical advantages of fast tumor formation and high tumor formation rate are manifested; on the otherhand, in the process of passage, the method cooperates with the cryopreserved operation of a relevant tumor tissue culture solution, and the lymphoma animal model for passage can be recovered at anymoment and directly constructed.

The invention discloses a method for establishing a model of oral cancer and lymphatic metastasis of mice, which is characterized by including mouse selection and grouping, reagent preparation, mouse feeding, experimental observation and conclusion drawing. The method specifically includes: using 200mg/L 4-nitroquinoline-1-oxide to feed Balb/c (Bagg albino/c) mice for 20 weeks by a drinking method; continuously using tap water to feed the mice to the fortieth week and observing to the fortieth week; obtaining the model of oral cancer and lymphatic metastasis of the mice which have development metastasis extremely similar to human oral cancer, namely location diversification, a full metastasis process of normal mucosa, dysplasia, carcinoma in situ, squamous carcinoma and cervical lymph nodes. The method is simple, natural and ideal in effect, guarantees high morbidity, diversified disease locations (lip, palate, gum, tongue and mouth floor) and high submandibular lymph node metastasis rate, and a novel powerful tool for preventing and treating human oral cancer is provided.

The invention relates to the field of medicines, relates to a method for establishing an animal model, and particularly relates to a method for establishing a human colon cancer transplanted carcinomamouse model. A preclinical tumor model is made through preprocessing mice with prostaglandin E2 and inoculating immunodeficient mice (Balb/c or NOD/SCID) with the in-vitro in-situ tumors of a patientagainst the defects of low establishing success rate and instability of existing colon cancer models. The invention provides a method for improving the success rate of a colon cancer PDX model, and especially provides the method for preparing the high-infiltration and high-metastasis stable human colon cancer transplanted carcinoma mouse model. The model has great values for preclinical evaluation, treatment and prognosis of the colon cancer.

The invention discloses application of a RAG2 gene knockout rat in establishing a personalized tumor treatment model. The personalized humanized RAG2 gene knockout rat tumor treatment model is successfully established by transplanting tumor tissues of a patient by using the RAG2 gene knockout rat as a carrier. In the treatment model, tumor models with different abnormal paths are established, so that the condition of the tumor disease of the patient is trustily simulated. Drugs and treatment methods with tumor-resisting functions are evaluated by virtue of characteristics of each patient. The method for establishing the personalized tumor treatment model by virtue of the RAG2 gene knockout rat is simple in step, and the established RAG2 gene knockout rat tumor treatment model has the advantages of being short in period, high in tumor formation rate and low in expense, and can be used for forming tumors for different types of tumor tissues, so that the model can be widely applied to personalized treatment of tumors.

The invention discloses a human esophageal carcinoma cell line and application thereof. The human esophageal carcinoma cell line is preserved in China Center For Type Culture Collection with a preservation number of CCTCC NO:C201661. Under the premise of retaining the main clinical biological features, the human esophageal carcinoma cell line has the characteristics of high tumor formation rate, short incubation period, good homogeneity and the like, enriches the human esophageal carcinoma cell library, provides powerful scientific research material for study of China population genetic background, and also provides a new experimental material for new drug preclinical study of in vivo and in vitro experiments.

The invention discloses a human gastric cancer cell line with 5-fluorouracil resistance and an establishing method and application thereof. The human gastric cancer cell line is preserved in China Center for Type Culture Collection and has a preservation number of CCTCC NO:2013154. The human gastric cancer cell line has the advantages of stable characters, stable multiple passages, high tumor formation rate, short incubation period and good uniformity, and provides a novel experimental material closer to the biological characteristics of clinical tumor for the study of gastric carcinoma. The human gastric cancer cell line has tumorigenicity; the generated tumor has 5-fluorouracil resistance and can be used to analyze the in vitro and in vivo drug sensitivity and resistance correlation, and then establish in vitro and in vivo two related drug screening platforms; and the is cell line is ideal for basic research of gastric cancer and clinical early stage application.

The invention discloses a preparation method of a targeted exosome carrying an anti-tumor protein and application thereof. The exosome is extracted from a sample by adopting the preparation method of the targeted exosome carrying the anti-tumor protein. The exosome is separated and purified, and exosome capsule particles are extracted. The growth of tumors can be efficiently inhibited, a potential ideal treatment method is provided for malignant tumor treatment, the exosome is extracted by utilizing a separation and purification method, separation can be conveniently, efficiently and standardly performed, the process is simple, and operation is convenient. The working efficiency is improved.

The invention discloses a preparation method of an in-situ primary gastric cancer tumor model, and belongs to the field of tumor animal models. The method comprises the following steps: culturing mouse gastric epithelial cells into organoids by using a specific culture medium, carrying out genetic modification on the organoids, and injecting the modified organoids back to the mouse stomach so as to develop the organoids into tumors. Compared with a transplanted tumor animal model, the method provided by the invention has a clear gene mutation background, and can effectively simulate the real process of occurrence and development of human gastric cancer. Compared with a genetic engineering tumor animal model, the gastric cancer mouse model with different gene mutation backgrounds can be efficiently constructed, the consumed time is short, the cost is low, and the tumor formation rate is high.

The embodiment of the invention discloses a method for establishing a standardized tumor animal model and a culture device. The method comprises the following steps that a 3D cell ring culture mold ispretreated, and the pretreated 3D cell ring culture mold is placed into a cell culture dish; the 3D cell ring culture mold is obliquely rotaed in the space of the cell culture dish according to different sequences, and 3D tumor cell rings with uniformly distributed tumor cells are cultured on the 3D cell ring culture mold; and the 3D tumor cell ring is cultured in vitro until the 3D tumor cell ring is formed, the 3D tumor cell ring is transplanted into the animal tissue for secondary culture, and the tumor cells of the 3D tumor cell ring are enabled to bidirectionally grow at the modeling position of the animal tissue until the tumor grows and is formed. According to the scheme, cell loss is little, the tumor formation rate is high, tumor formation is uniform and stable, the number of thecells required by modeling and the modeling cost are greatly reduced, and the method for establishing the standardized tumor animal model is efficient, simple, reliable, high in operability, economical and practical.

The invention discloses a construction method of a human prostatic cancer fresh tissue hormone-independent prostatic cancer nude mouse model. The construction method comprises the steps of subcutaneously hiding a slow-release capsule which contains 10mg of testosterone in a mouse for a week, mixing primary cells which are cultured by human prostatic cancer fresh tissues with an artificial basement membrane, inoculating to the unilateral renal capsule of a BULB/C nude mouse which is replenished with androgen, passaging to the prostate anterior lobe of the mouse to obtain a human hormone-dependent prostatic cancer nude mouse model, carrying out castration until the tumor of the nude mouse grows to be greater than 50mm<3>, passaging the tumor to a nude mouse which is surgically castrated and not replenished with androgen, and after the tumor grows up, carrying out passage to obtain the human prostatic cancer fresh tissue hormone-independent prostatic cancer nude mouse model. In such way, the human prostatic cancer fresh tissue hormone-independent prostatic cancer nude mouse model is closer to the human prostatic cancer; and by using a technology of planting human prostatic cancer fresh tissue via nude mouse variants and a primary cell culture technology as platforms, hormone-dependent and hormone-independent prostatic cancer nude mouse models with the same prostatic cancer tissue source are obtained at the same time.

The embodiment of the invention provides a construction method of a nasopharyngeal carcinoma tumor model. The construction method comprises the following steps of inoculating nasopharyngeal carcinomacell line suspension under the skin of a non-human animal, and culturing; after the non-human animal bears the tumor, taking a nasopharyngeal carcinoma tumor cultured subcutaneously, and cutting the nasopharyngeal carcinoma tumor into tumor tissue blocks; inoculating the tumor tissue blocks on the nasopharynx of a normal non-human animal by using an inoculating needle; and adhering an inoculationport and the tumor tissue blocks by using tissue adhesive by using an inoculation needle, and culturing.

The invention discloses a construction method of an in-situ liver cancer model of SD rats (Sprague-Dawley) having a high liver hardness background, and an application thereof. The construction method comprises the following steps: performing hypodermic injection of carbon tetrachloride on the abdomen of SD rats to induce the formation of high liver hardness on the SD rats, injecting Buffalo rat liver cancer cells mixed with low-growth factor Matrigel under a liver envelope of the SD rats, performing short-term intramuscular injection in combination with a small dose of glucocorticoid , and forming an in-situ liver cancer model of the SD rats having a high liver hardness background after 12-14 days. SD rats and rat liver cancer cells adopted by the method are easy to obtain and low in cost, and have the advantages of high tumor formation rate, controllable tumor number and position, short tumor formation time, high metastasis and the like, so that the problems of technical difficulty and modeling cost of the establishment of a hardness background liver cancer animal model in the prior art are effectively solved, malignant pathological characteristics of liver cancer patients having a high liver hardness background are well simulated, and a practical and efficient novel liver cancer animal model is provided for analysis of mechanisms such as gene functions, radiotherapy resistance, and immunosuppression in a hardness mechanical micro-environment.