Method for establishing human tumor xenotransplantation model cultured in vitro

Abstract

The invention relates to the field of human xenotransplantation model research, in particular to a method for establishing a human tumor xenotransplantation model cultured in vitro. The establishing method includes the following steps: (1) collecting a tumor tissue sample; (2) placing a tumor tissue in a culture solution; (3) completing the preparation for low-temperature transportation and treatment; (4) cutting the tumor tissue into grain size in a sterile operating table; (5) cleaning the tumor tissue and then placing the tumor tissue in a hole plate 48 for culture; (6) detecting tissue apoptosis by in situ end labeling method; (7) selecting immunocompromised NCG mice to perform anesthesia and skin treatment; (8) implanting the cultured tumor tissue into the renal capsule of the mice; (9) completing modeling after 3 months. According to the invention, tumor tissue blocks are selected for modeling for the xenotransplantation model, which is beneficial to preserving the histopathological and genetic characteristics of primary tumors; and moreover, the obtained tumor tissue are cultured in vitro, which is beneficial to tumor typing and clinical practice; the addition of PD 98059 and artificial matrix glue can greatly reduce tissue apoptosis, which is beneficial to improving the success rate of transplantation.

Description

technical field [0001] The invention relates to the research field of a human-derived xenograft model, in particular to a method for establishing a human-derived tumor xenograft model cultured in vitro. Background technique [0002] Cancer is a major public health problem worldwide, and its morbidity and mortality are increasing year by year. At the same time, the incidence of cancer is the main cause of death today (Siegel R L et al., 2017); A model that highly simulates the occurrence and development of tumors in the human body is a key step in tumor research; currently, in vivo mouse models are used to simulate the development of tumors in humans, and the currently used in vivo models of mice include genetic engineering mice, chemically induced Models and human-derived xenograft models, among which the human-derived xenograft model (English: patient-derived xenografts, abbreviated as PDX) is based on the inoculation of fresh tumor cells or tissues from patients into immun...

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