52results about How to "Improve the success rate of transplantation" patented technology

The invention belongs to the field of histocytology and tissue engineering and discloses a tissue-engineered epidermis with hypodermal cells and a preparation method thereof. The method for preparing the tissue-engineered epidermis with hypodermal cells comprises the following steps: obtaining epidermis cells; inoculating the obtained epidermis cells, culturing the cells until the density is 80-100 percent by using an epidermis culture medium, replacing the medium into a mixed culture medium, culturing for 16-24 hours, and collecting the epidermis cell layer, thereby obtaining the tissue-engineered epidermis piece; transferring the obtained tissue-engineered epidermis onto a semi-permeable polymer or a silicone mold substrate or a petrolatum gauze, thereby obtaining a film with the epidermis cell layer; and culturing the hypodermal cells, paving the obtained hypodermal cells on the epidermis cell layer with the film of the epidermis cell layer, and culturing for 1-2 hours, thereby obtaining the product. The preparation method provided by the invention is easy to operate and short in period, the tissue-engineered epidermis can be mutually stimulated and induced with receptor skin tissues, and formation of a complete epidermal structure of the receptor is promoted.

The invention relates to the field of human xenotransplantation model research, in particular to a method for establishing a human tumor xenotransplantation model cultured in vitro. The establishing method includes the following steps: (1) collecting a tumor tissue sample; (2) placing a tumor tissue in a culture solution; (3) completing the preparation for low-temperature transportation and treatment; (4) cutting the tumor tissue into grain size in a sterile operating table; (5) cleaning the tumor tissue and then placing the tumor tissue in a hole plate 48 for culture; (6) detecting tissue apoptosis by in situ end labeling method; (7) selecting immunocompromised NCG mice to perform anesthesia and skin treatment; (8) implanting the cultured tumor tissue into the renal capsule of the mice; (9) completing modeling after 3 months. According to the invention, tumor tissue blocks are selected for modeling for the xenotransplantation model, which is beneficial to preserving the histopathological and genetic characteristics of primary tumors; and moreover, the obtained tumor tissue are cultured in vitro, which is beneficial to tumor typing and clinical practice; the addition of PD 98059 and artificial matrix glue can greatly reduce tissue apoptosis, which is beneficial to improving the success rate of transplantation.

The invention discloses a tissue-engineered cornea and a preparation method thereof. The tissue-engineered cornea comprises three or more layers of laminated structures, each layer of the laminated structure is a composite layer formed in the mode that a fiber scaffold prepared by electrospinning or electrostatic direct writing or light curing 3D printing is cured and composited with one or more of collagen, an acellular matrix and silk fibroin, the space between every two adjacent layers is inoculated with corneal stromal cells and neurons, the corneal stromal cells and the neurons can grow in the direction of fibers of the scaffold, the corneal stromal cells between every two adjacent layers secrete extracellular matrixes to form a tissue structure similar to the human corneal stroma, and the upper surfaces and the lower surface of the laminated structures are inoculated with corneal epithelial cells and corneal endothelial cells. The tissue-engineered cornea can simulate the corneastructure well, is easy to prepare and good in biocompatibility and is easily fused with tissue.

The invention relates to a hollow heterotype bacteria cellulose artificial blood vessel stent material with a gradient structure and a preparation method thereof. The artificial blood vessel stent with a 'compact inner cavity, elastic inside, loose outer wall' biological structure similar to a human blood vessel tissue can be prepared by controlling the culture condition of bacteria cellulose, and has excellent biocompatibility and cell viability, wherein the combination between a compact layer and an elastic layer and between the elastic layer and a loose layer can be in a further degree, and not limited to the surface of the material; physical layering is not remarkable, the structure continuity is excellent, and the 'compact inside and loose outside' in the human blood vessel tissue can be furthest imitated due to the gradient changing structure in the compact layer, the elastic layer and the loose layer so that the time for culturing cells in vitro can be shortened, the rejecting phenomenon of a human body to a foreign transplant can be remarkably reduced, the transplant success rate can be greatly improved, the stent forming process is simple, the culture period is short, preparation process is green and environment-friendly, easy and quick, and the preparation cost is low.

Proceeding from key mangrove afforesting links, such as standard of natural nursery stocks, specification of nursery stock soil balls, transportation treatment measures, seasons and times for transplanting and afforesting, use of root promoting auxin, digging and planting, management measures and the like, the invention provides a whole set of effective method for transplanting and afforesting large seedlings of natural mangrove plants. By using the method disclosed by the invention, practices prove that the natural seedling transplanting success rate can be increased, the forestation time can be greatly shortened, ecological functions of mangroves can be rapidly realized, the forestation fund can be saved, the mangroves can be effectively managed, and the mangrove forestation success rate in our country can be increased, so that rapid recovery of the mangroves in our country can be promoted.

The invention discloses tissue engineering skin with a layered structure and a preparation method of the tissue engineering skin, and relates to the technical field of tissue engineering skin. The tissue engineering skin with the layered structure comprises a collagen layer, a collagen layer embedded in human skin fibroblast, a first multilayer collagen layer, a collagen layer embedded in keratinocyte and a second multilayer collagen layer. According to the tissue engineering skin disclosed by the invention, a manner that channels are innovatively generated in tissues by a temporary material such as gelatin is adopted, and human umbilical vein endothelial cells are adhered to the inner parts of the channels, so that the formed blood vessels are better fit with human body blood vessels; thetransporting and distributing rate of the formed blood vessels is increased; cells have higher viability and high multiplication rate; the printed skin with the layered structure is adopted, so thatthe interaction between cells of the formed skin structure and the interaction of the skin tissues and epimatrix are more stable, and regeneration of the skin tissue is facilitated.

The invention provides a tissue engineering skin with myocyte and the preparation method of the tissue engineering skin. The invention has the characteristics that the prepared skin is made from the myocyte, skin fibroblast, skin epithelial cell and biomaterial scaffold; the invention is the tissue engineering skin with active myocyte, which is formed by compounding the myocyte with the skin fibroblast at the inside the biomaterial scaffold and planting the epithelial cell on the surface of the biomaterial scaffold. The prepared tissue engineering skin with myocyte is similar as the skin of human body and has the advantages of increasing the skin elasticity and mechanical performance, preventing body fluid flowing away, protecting the wound surface, promoting the wound surface healing and resisting the cicatrisation.

The invention discloses a sealing housing protected anal sphincter prosthesis, and belongs to the technical field of medical apparatuses. The sealing housing protected anal sphincter prosthesis comprises a coil box, a prosthesis clamping mechanism as well as a left sealing housing and a right sealing housing, wherein the prosthesis clamping mechanism is sequentially provided with an upper fixing ring, an arc-shaped swinging leg and a lower fixing ring in an axial direction, and the left sealing housing and the right sealing housing cover two sides of the prosthesis clamping mechanism and are fixedly connected; at the outer side of an annular structure, a driving device is encapsulated on the prosthesis clamping mechanism in an axial direction; the arc-shaped swinging leg is connected to the driving device; an electric wire passing drum is arranged at the bottom of the driving device; and the electric wire passing drum runs through the right sealing housing and is in sealed connection to the coil box by virtue of a silicone tube. According to the sealing housing protected anal sphincter prosthesis provided by the invention, by arranging the sealing housings at the outer side of the clamping mechanism, a contact area of the prosthesis and biological tissues is greatly diminished and smoothness of the outer surface of the mechanism (the prosthesis) is improved, so that mechanism failure, due to tissue exclusive hyperplasia and adhesion after implantation of the mechanism, is prevented.

The invention discloses a double-layer artificial skin and a preparation method thereof, wherein, cell-free membranous biological derivative material is used as a surface layer, and a fibroblast, extracellular matrix synthesized and secreted by the fibroblast and a cell growth factor are compounded in the interior of biological support material to form a dermis, and then the surface layer and thedermis are combined to form the double-layer artificial skin; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as the infection of the surface of wound, and be beneficial to epitheliosis and epithelial growth; the dermis can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; the artificial skin has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface ofwound is healed, reducing excess scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the double-layer artificial skin has wide material resources and simple production method, and is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

The invention discloses a seaweed plant transplanting device and a transplanting method, which relate to the technical field of ecological engineering and mainly include a seedling storage mechanism, an insertion mechanism, a walking frame and a driving mechanism. The invention not only realizes the mechanized transplantation of seaweed plants, but also the row spacing, plant spacing, and insertion depth of the plants can be adjusted and controlled, and the efficiency can reach 2000 transplanting units / person / day, and the transplanting efficiency has been greatly improved compared with manual transplantation. The efficiency of manual transplantation is increased by 233%, which is significantly higher than that of other current seagrass transplanting machines; in addition, the invention realizes the miniaturization of the seaweed transplantation device, light weight, simple operation, high success rate of transplantation, and a single person can complete the whole eelgrass Transplanting work; the transplanting method of the present invention has little damage to the natural grass bed, and only gathers vegetative branches with rhizomes to form a transplant unit through binding. Environment friendly.

The invention provides a self-help hematopoietic stem cell matching method and device. The self-help hematopoietic stem cell matching method includes that when an initiating terminal initiates a request to a self-help response module, the self-help response module automatically analyzes number of the terminal; the self-help response module feeds back to the initiating terminal to require the initiating terminal to provide request information needed for matching; the self-help response module feeds matching operation information back to the initiating terminal. By the self-help hematopoietic stem cell matching method and device, a patient is enabled to perform hematopoietic stem cell matching retrieval timely at all times and places, updated matching can be timely sent to a mobile terminal of the patient after data are updated, convenience is brought to clinic treatment, and survival probability of the patient is increased.

The invention discloses a dipyridamole-loaded polyurethane anticoagulative material and a preparation process thereof. The material adopts a core-shell structure, the inner layer is a dipyridamole loaded layer, and the outer layer is a polyurethane main body layer. When the material is used as an artificial blood vessel to be transplanted, the dipyridamole can be slowly released in a long term, adhesion, aggregation and activation of platelets on the surface of the artificial blood vessel are inhibited, the blood compatibility of the artificial blood vessel is improved, the acute thrombosis and intima proliferation can be effectively avoided, and the transplanting success rate of the artificial blood vessel can be effectively improved.

A sealed shell-protected anal sphincter prosthesis in the technical field of medical devices, comprising: a coil box, a prosthesis clamping mechanism provided with an upper fixing ring, an arc-shaped swing leg and a lower fixing ring in sequence from top to bottom in the axial direction; The left sealing shell and the right sealing shell covering both sides of the prosthesis clamping mechanism, wherein: the left sealing shell and the right sealing shell are fixedly connected, and the prosthesis clamping mechanism is axially encapsulated with a driving device outside the ring structure, and the arc The shaped swing leg is connected with the driving device, and the bottom of the driving device is provided with a wire passing barrel, and the wire passing barrel passes through the right sealing shell and is sealed and connected with the coil box through a silicone tube. The present invention greatly reduces the contact area with biological tissues by installing a sealed shell on the outside of the clamping mechanism, improves the smoothness of the outer surface of the mechanism, thereby avoiding mechanism failures caused by tissue rejection hyperplasia and adhesion after the mechanism is implanted.

The invention provides a bionic double-layer dressing, which comprises a decellularized animal small intestinal submucosa and a gel layer cross-linked on the film layer, and the film layer and the gel layer together form a bionic double-layer dressing with a double-layer structure; The preparation steps include preparation of decellularized small intestinal submucosa, preparation of decellularized small intestinal submucosa powder, preparation of decellularized small intestinal submucosa solution and preparation of bionic double-layer dressing. To the surface of the submucosa of the decellularized small intestine, the bionic double-layer dressing was obtained after freezing and cross-linking. The bionic double-layer dressing prepared by the invention not only has excellent tensile properties, but also has a semi-permeable barrier, which can prevent water evaporation and bacterial penetration. At the same time, the bionic double-layer dressing prepared by the invention is beneficial to the healing of the wound, can significantly increase the healing speed of the wound, and the wound healing rate is obviously accelerated on the 3rd day and the 7th day after the operation.

A contact-type artificial anal sphincter prosthesis limit protection switch device, comprising: a motor casing, a transmission mechanism arranged in the motor casing, a swing arm mechanism, a limit mechanism and a controller, wherein: the swing arm mechanism and the transmission mechanism Connected, the limit mechanism includes: two limit switches connected to the controller respectively arranged on the swing arm mechanism and the motor casing, the first limit switch and the second limit switch are both switch round buttons and connected in series A limit switch circuit composed of a 10K resistor, the limit switch circuit leads to a high-level wire, a signal wire and a ground wire. In the present invention, by setting the limit switch, the switch round button can be pressed at the limit position where the swing arm of the lower ring swings to the left and right, respectively, to trigger the limit signal, and the limit signal generated by the limit switch will be transmitted to the controller for further processing. processing, simple structure, easy to realize, and high stability.

The invention discloses 2,7-substituted 3H-imidazole[4,5-b]pyridine compounds shown as the structural formula I, or pharmaceutically acceptable salt, ester and precursors, and / or hydrate and medicinal compositions thereof, and a preparation method for the compounds. The compounds, or pharmaceutically acceptable salt, ester and precursors, and / or hydrate and medicinal compositions thereof can serve as antagonists of CR4 and are used for treating or preventing CXCR4 receptor activation related diseases.

The invention belongs to the field of histocytology and tissue engineering and discloses a tissue-engineered epidermis with hypodermal cells and a preparation method thereof. The method for preparing the tissue-engineered epidermis with hypodermal cells comprises the following steps: obtaining epidermis cells; inoculating the obtained epidermis cells, culturing the cells until the density is 80-100 percent by using an epidermis culture medium, replacing the medium into a mixed culture medium, culturing for 16-24 hours, and collecting the epidermis cell layer, thereby obtaining the tissue-engineered epidermis piece; transferring the obtained tissue-engineered epidermis onto a semi-permeable polymer or a silicone mold substrate or a petrolatum gauze, thereby obtaining a film with the epidermis cell layer; and culturing the hypodermal cells, paving the obtained hypodermal cells on the epidermis cell layer with the film of the epidermis cell layer, and culturing for 1-2 hours, thereby obtaining the product. The preparation method provided by the invention is easy to operate and short in period, the tissue-engineered epidermis can be mutually stimulated and induced with receptor skin tissues, and formation of a complete epidermal structure of the receptor is promoted.