52results about How to "Improve the success rate of transplantation" patented technology

The invention relates to tissue engineering skin and a preparation method thereof. The tissue engineering skin comprises a dermis layer and an epidermis layer, and is prepared from seed cells, cell growth factors and a biological scaffold material according to a biological 3D printing technology. The tissue engineering skin is similar to natural skin, comprises the dermis layer and the epidermis layer, can be produced in batches, can be closely attached to a wound, and has high biocompatibility, the dermis layer contains blood vessels, sweat glands and hair follicles, the shape, size and thickness of the tissue engineering skin can be easily changed, the elasticity and flexibility of the skin after sound healing can be enhanced, and the tissue engineering transplantation success rate can be increased.

The invention belongs to the field of histocytology and tissue engineering and discloses a tissue-engineered epidermis with hypodermal cells and a preparation method thereof. The method for preparing the tissue-engineered epidermis with hypodermal cells comprises the following steps: obtaining epidermis cells; inoculating the obtained epidermis cells, culturing the cells until the density is 80-100 percent by using an epidermis culture medium, replacing the medium into a mixed culture medium, culturing for 16-24 hours, and collecting the epidermis cell layer, thereby obtaining the tissue-engineered epidermis piece; transferring the obtained tissue-engineered epidermis onto a semi-permeable polymer or a silicone mold substrate or a petrolatum gauze, thereby obtaining a film with the epidermis cell layer; and culturing the hypodermal cells, paving the obtained hypodermal cells on the epidermis cell layer with the film of the epidermis cell layer, and culturing for 1-2 hours, thereby obtaining the product. The preparation method provided by the invention is easy to operate and short in period, the tissue-engineered epidermis can be mutually stimulated and induced with receptor skin tissues, and formation of a complete epidermal structure of the receptor is promoted.

The invention relates to the field of human xenotransplantation model research, in particular to a method for establishing a human tumor xenotransplantation model cultured in vitro. The establishing method includes the following steps: (1) collecting a tumor tissue sample; (2) placing a tumor tissue in a culture solution; (3) completing the preparation for low-temperature transportation and treatment; (4) cutting the tumor tissue into grain size in a sterile operating table; (5) cleaning the tumor tissue and then placing the tumor tissue in a hole plate 48 for culture; (6) detecting tissue apoptosis by in situ end labeling method; (7) selecting immunocompromised NCG mice to perform anesthesia and skin treatment; (8) implanting the cultured tumor tissue into the renal capsule of the mice; (9) completing modeling after 3 months. According to the invention, tumor tissue blocks are selected for modeling for the xenotransplantation model, which is beneficial to preserving the histopathological and genetic characteristics of primary tumors; and moreover, the obtained tumor tissue are cultured in vitro, which is beneficial to tumor typing and clinical practice; the addition of PD 98059 and artificial matrix glue can greatly reduce tissue apoptosis, which is beneficial to improving the success rate of transplantation.

The invention discloses 2,7-substituted 3H-imidazole[4,5-b]pyridine compounds shown as the structural formula I, or pharmaceutically acceptable salt, ester and precursors, and / or hydrate and medicinal compositions thereof, and a preparation method for the compounds. The compounds, or pharmaceutically acceptable salt, ester and precursors, and / or hydrate and medicinal compositions thereof can serve as antagonists of CR4 and are used for treating or preventing CXCR4 receptor activation related diseases.

A position limitation protection type ani sphincter prosthesis comprises an upper fixed ring arranged on an upper ring sleeve in a sleeving mode, a movable middle ring arranged on a middle ring sleeve in a sleeving mode and a lower fixed ring arranged on a motor sleeve in a sleeving mode, wherein the upper fixed ring, the movable middle ring and the lower fixed ring are sequentially connected, the upper ring sleeve is axially connected with the middle ring sleeve, a reduction gearbox connected with a motor is arranged between the middle ring sleeve and the upper ring sleeve, the movable middle ring is connected with a reduction gear train in the reduction gearbox through an inner gear ring arranged on a gear ring sleeve in a sleeving mode, a Hall element is arranged on an upper cover plate of the reduction gearbox, and two magnets matched with the Hall element are arranged at the opposite ends of the gear ring sleeve and the upper cover plate of the reduction gearbox in a spaced mode. It can be effectively avoided that intestines are damaged due to the too large swing amplitude of a swing arm of the middle ring, a mechanical is stuck due to the overturning of the motor, and the motor is burnt down due to the too large current; the opening and closing amplitude of the prosthesis can be adjusted by a mechanism according to the feeling of human intestines; the whole device is simple in structure, small in size, light in weight and beneficial for increasing the transplanting success rate.

The invention discloses a tissue-engineered cornea and a preparation method thereof. The tissue-engineered cornea comprises three or more layers of laminated structures, each layer of the laminated structure is a composite layer formed in the mode that a fiber scaffold prepared by electrospinning or electrostatic direct writing or light curing 3D printing is cured and composited with one or more of collagen, an acellular matrix and silk fibroin, the space between every two adjacent layers is inoculated with corneal stromal cells and neurons, the corneal stromal cells and the neurons can grow in the direction of fibers of the scaffold, the corneal stromal cells between every two adjacent layers secrete extracellular matrixes to form a tissue structure similar to the human corneal stroma, and the upper surfaces and the lower surface of the laminated structures are inoculated with corneal epithelial cells and corneal endothelial cells. The tissue-engineered cornea can simulate the corneastructure well, is easy to prepare and good in biocompatibility and is easily fused with tissue.

The invention relates to a hollow heterotype bacteria cellulose artificial blood vessel stent material with a gradient structure and a preparation method thereof. The artificial blood vessel stent with a 'compact inner cavity, elastic inside, loose outer wall' biological structure similar to a human blood vessel tissue can be prepared by controlling the culture condition of bacteria cellulose, and has excellent biocompatibility and cell viability, wherein the combination between a compact layer and an elastic layer and between the elastic layer and a loose layer can be in a further degree, and not limited to the surface of the material; physical layering is not remarkable, the structure continuity is excellent, and the 'compact inside and loose outside' in the human blood vessel tissue can be furthest imitated due to the gradient changing structure in the compact layer, the elastic layer and the loose layer so that the time for culturing cells in vitro can be shortened, the rejecting phenomenon of a human body to a foreign transplant can be remarkably reduced, the transplant success rate can be greatly improved, the stent forming process is simple, the culture period is short, preparation process is green and environment-friendly, easy and quick, and the preparation cost is low.

Proceeding from key mangrove afforesting links, such as standard of natural nursery stocks, specification of nursery stock soil balls, transportation treatment measures, seasons and times for transplanting and afforesting, use of root promoting auxin, digging and planting, management measures and the like, the invention provides a whole set of effective method for transplanting and afforesting large seedlings of natural mangrove plants. By using the method disclosed by the invention, practices prove that the natural seedling transplanting success rate can be increased, the forestation time can be greatly shortened, ecological functions of mangroves can be rapidly realized, the forestation fund can be saved, the mangroves can be effectively managed, and the mangrove forestation success rate in our country can be increased, so that rapid recovery of the mangroves in our country can be promoted.

The invention discloses tissue engineering skin with a layered structure and a preparation method of the tissue engineering skin, and relates to the technical field of tissue engineering skin. The tissue engineering skin with the layered structure comprises a collagen layer, a collagen layer embedded in human skin fibroblast, a first multilayer collagen layer, a collagen layer embedded in keratinocyte and a second multilayer collagen layer. According to the tissue engineering skin disclosed by the invention, a manner that channels are innovatively generated in tissues by a temporary material such as gelatin is adopted, and human umbilical vein endothelial cells are adhered to the inner parts of the channels, so that the formed blood vessels are better fit with human body blood vessels; thetransporting and distributing rate of the formed blood vessels is increased; cells have higher viability and high multiplication rate; the printed skin with the layered structure is adopted, so thatthe interaction between cells of the formed skin structure and the interaction of the skin tissues and epimatrix are more stable, and regeneration of the skin tissue is facilitated.

The invention discloses a preparation method of a polyglycolic acid and pig islet compound, relates to a preparation method of a pig islet transplanting compound, and solves the problems that as current in vitro cultivating environment of pig islet is different from in vivo environment, pig islet cells have difficult in vitro survival and uneasy in vitro cultivation. The preparation method of the polyglycolic acid and pig islet compound comprises the following steps: 1. polyglycolic acid stent is processed; and 2. three-dimensional micro-gravity cells are cultivated. The polyglycolic acid and pig islet compound that is prepared by the preparation method raises the in vitro cultivating quality and in vitro survival time of pig islet cells, can improve the activity and insulin secreting function of pig islet cells, increases the number of pig islet cells with activity and insulin secreting function, and then achieves the goal for raising islet transplanting success ratio and provides physical foundation for curing diabetes mellitus in future.

The invention provides bionic double-layer dressing, comprising acellular animal intestinal submucosa and a gel layer crosslinked to the same. The acellular animal intestinal submucosa and the gel layer jointly form the bionic double-layer dressing in double-layer structure. Preparation steps include preparing the acellular animal intestinal submucosa, preparing powder of the acellular animal intestinal submucosa, preparing a solution of the acellular animal intestinal submucosa, preparing the bionic double-layer dressing; the solution of the acellular animal intestinal submucosa is mixed witha crosslinking liquid, the mixture is added to the surface of the acellular animal intestinal submucosa, and freezing and crosslinking are performed to obtain the bionic double-layer dressing. The bionic double-layer dressing has excellent tension resistance, has semipermeable barrier, can prevent moisture evaporation and bacterial penetration, is good for healing of a wound, and enables wound healing speed to be evidently increased, with the wound healing speed evidently increased on third to seventh day after surgery.

The invention relates to the technical field of medical equipment, and in particular relates to an isolated organ storage system, which comprises a donor blood collection device, a complementary-pull type antiphase beating pump, a first roller single pump, an oxygenator, an isolated organ thermal insulation device, a blood heating device, an oxygen supply device, a filter, a waste liquid bag, an urine collection device, and a bile collection device. The isolated organ storage system of the invention is based on a novel blood pump (i.e. complementary-pull type antiphase beating pump), and integrates the membrane type oxygenator, the filter, the roller single pump and necessary monitoring sensors. The invention provides a pseudo-ecological isolated organ storage method and storage system. To this end, the invention in particular provides the corresponding isolated organ obtaining method and the thermal insulation and heating device. The invention aims to maximally prolong the storage time of isolated organs (liver, kidney) to facilitate the transportation and repair, thereby improving transplantation success rate and receptor survival rate.

The invention relates to a pharmaceutical composition, comprising the medicinal ingredients of mesenchymal stem cells (MSCs) of expressing SDF-1 / HOXB4 fusion gene and CD34<+> hematopoietic stem cells (HSCs). An adenoviral expression vector is built by the invention; the SDF-1, HOXB4 and fuse protein SDF-1 / HOXB4 thereof are respectively expressed; the effects of cotransplantation of the mesenchymal stem cells of expressing the SDF-1 / HOXB4 fusion gene combined with the fetal blood CD34<+> hematopoietic stem cells on radiation-injured mice are observed. The result shows that hematopoietic reconstitution and implantation can be facilitated by cotransplantation of the mesenchymal stem cells (MSCs) of expressing the SDF-1 / HOXB4 fusion gene combined with the fetal blood CD34<+> hematopoietic stem cells (HSCs), and the success rate of transplantation is also improved.

The invention provides a separation and extraction fluid suitable for pig pancreas islet and a preparation thereof. The fluid is characterized in that the oxygen load of the separation and extraction fluid is high and the problem of oxygen deficit in the separation and extraction process of pancreas islet can be solved. The separation and extraction fluid comprises the following steps of: first, taking a basal culture medium or a balanced salt solution as an aqueous phase; and adding collagenase, calcium chloride, DNA enzyme, bovine serum albumin, perfluorocarbon, an emulgator and an antibacterial agent for meeting the demand of separating and extracting pig pancreas islet. The separation and extraction fluid provided by the invention cannot release the anoxic degree in the separation and extraction process of pancreas islet and the survival rate, the function and the activity of the pancreas islet treated by the separation and extraction fluid provided by the invention are superior to those of pancreas islet treated by the conventional separation fluid with collagenase only.

The invention provides a tissue engineering skin with myocyte and the preparation method of the tissue engineering skin. The invention has the characteristics that the prepared skin is made from the myocyte, skin fibroblast, skin epithelial cell and biomaterial scaffold; the invention is the tissue engineering skin with active myocyte, which is formed by compounding the myocyte with the skin fibroblast at the inside the biomaterial scaffold and planting the epithelial cell on the surface of the biomaterial scaffold. The prepared tissue engineering skin with myocyte is similar as the skin of human body and has the advantages of increasing the skin elasticity and mechanical performance, preventing body fluid flowing away, protecting the wound surface, promoting the wound surface healing and resisting the cicatrisation.

The invention discloses a sealing housing protected anal sphincter prosthesis, and belongs to the technical field of medical apparatuses. The sealing housing protected anal sphincter prosthesis comprises a coil box, a prosthesis clamping mechanism as well as a left sealing housing and a right sealing housing, wherein the prosthesis clamping mechanism is sequentially provided with an upper fixing ring, an arc-shaped swinging leg and a lower fixing ring in an axial direction, and the left sealing housing and the right sealing housing cover two sides of the prosthesis clamping mechanism and are fixedly connected; at the outer side of an annular structure, a driving device is encapsulated on the prosthesis clamping mechanism in an axial direction; the arc-shaped swinging leg is connected to the driving device; an electric wire passing drum is arranged at the bottom of the driving device; and the electric wire passing drum runs through the right sealing housing and is in sealed connection to the coil box by virtue of a silicone tube. According to the sealing housing protected anal sphincter prosthesis provided by the invention, by arranging the sealing housings at the outer side of the clamping mechanism, a contact area of the prosthesis and biological tissues is greatly diminished and smoothness of the outer surface of the mechanism (the prosthesis) is improved, so that mechanism failure, due to tissue exclusive hyperplasia and adhesion after implantation of the mechanism, is prevented.

The invention discloses a double-layer artificial skin and a preparation method thereof, wherein, cell-free membranous biological derivative material is used as a surface layer, and a fibroblast, extracellular matrix synthesized and secreted by the fibroblast and a cell growth factor are compounded in the interior of biological support material to form a dermis, and then the surface layer and thedermis are combined to form the double-layer artificial skin; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as the infection of the surface of wound, and be beneficial to epitheliosis and epithelial growth; the dermis can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; the artificial skin has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface ofwound is healed, reducing excess scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the double-layer artificial skin has wide material resources and simple production method, and is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

The invention discloses a seaweed plant transplanting device and a transplanting method, which relate to the technical field of ecological engineering and mainly include a seedling storage mechanism, an insertion mechanism, a walking frame and a driving mechanism. The invention not only realizes the mechanized transplantation of seaweed plants, but also the row spacing, plant spacing, and insertion depth of the plants can be adjusted and controlled, and the efficiency can reach 2000 transplanting units / person / day, and the transplanting efficiency has been greatly improved compared with manual transplantation. The efficiency of manual transplantation is increased by 233%, which is significantly higher than that of other current seagrass transplanting machines; in addition, the invention realizes the miniaturization of the seaweed transplantation device, light weight, simple operation, high success rate of transplantation, and a single person can complete the whole eelgrass Transplanting work; the transplanting method of the present invention has little damage to the natural grass bed, and only gathers vegetative branches with rhizomes to form a transplant unit through binding. Environment friendly.

The invention belongs to the technical field of medical care, and discloses a management control system based on in vitro fertilization-embryo transfer. The management control system is provided witha patient information acquisition module, a patient examination module, an information check module, an intelligent advice module, a treatment data processing module, and an information push module. The management control system based on in vitro fertilization-embryo transfer has obvious advantages. The identity information of a culture dish carrying a sperm, an egg and an embryo and a maternal identity identification ring during in vitro fertilization and embryo transfer is read by using the radio frequency identification technology through the information check module, and the read identityinformation is compared to issue a correctness signal or a wrongness signal, so that the accuracy of in vitro fertilization and embryo transfer is ensured. The intelligent advice module can provide intelligent advices for doctors and improve the success rate of transplantation.

The invention provides a corneal carrier material and a preparation method thereof and relates to the technical field of tissue engineering. The preparation method of the corneal carrier material, provided by the invention, comprises the steps of subjecting repeated-frozen-thawed human corneal stroma homogenate to decellularizing and then carrying out treatment molding. According to the method, the process is simple and convenient, medical resources are utilized reasonably, and thus, the method is applicable to large-scale production and clinical popularization and application. According to the method, homogeneous corneal stroma homogenate is used as a raw material, and an artificial corneal carrier material is reconstructed on the basis of reserving natural extracellular matrix componentsof cornea, so that the prepared corneal support material has good optical properties, a three-dimensional structure and biocompatibility. Through removing remaining antigenic components from cornea by decellularizing, the probability of an exclusive reaction after corneal transplantation can be lowered, and the success rate of transplantation is increased.

The invention relates to a method for growing watermelon seedlings in a field, which includes the following steps: pretreating watermelon seeds: the step includes disinfection and secondary soaking treatment of watermelon seeds to accelerate the germination of the seeds; growing the treated watermelon seeds into seedlings: in the step, the to-be-germinated seeds are grown in seedling-growing barrels, the seedling-growing barrels are put into growing troughs covered by light-transmitting films, seedling ventilation is guaranteed at noon during growing, the films are removed before seedling transplantation, so that the seedlings are irradiated by sunlight for 1 to 2 days, and thereby growing is complete; and transplanting the seedlings into a field. The growing method provides a most cost-efficient and reasonable means, each seedling is in a nutritional system alone, a plurality of seedlings cannot affect one another, the survival rate of the seedlings is increased, and moreover, the success rate of seedling transplantation is indirectly increased.

The invention provides a self-help hematopoietic stem cell matching method and device. The self-help hematopoietic stem cell matching method includes that when an initiating terminal initiates a request to a self-help response module, the self-help response module automatically analyzes number of the terminal; the self-help response module feeds back to the initiating terminal to require the initiating terminal to provide request information needed for matching; the self-help response module feeds matching operation information back to the initiating terminal. By the self-help hematopoietic stem cell matching method and device, a patient is enabled to perform hematopoietic stem cell matching retrieval timely at all times and places, updated matching can be timely sent to a mobile terminal of the patient after data are updated, convenience is brought to clinic treatment, and survival probability of the patient is increased.

The invention discloses a method for movement and rejuvenation of old trees and belongs to the field of transplantation of old trees and big trees. The method comprises the steps that 1, the old treesare subjected to mathematical modeling; 2, the crowns of the old trees are pruned; 3, according to a mathematical modeling result, lump digging contour lines are marked on the surrounding ground where the trunks are located, lump digging operation is conducted along the outer edges of the lump digging contour lines, root breaking grooves are dug accordingly, and cylindrical soil lumps are formed;4, the cylindrical soil lumps are subjected to root pruning operation, and apple-shaped soil lumps are formed; 5, beams are transversely inserted in the bottoms of the apple-shaped soil lumps in thehorizontal direction and connected to form a first base; 6, a second base is assembled on a carrying plate of a semitrailer; 7, hoisting equipment is used for hoisting the first base, the big trees are lifted accordingly, and the first base is moved to the carrying plate of the semitrailer; 8, the old trees are lain down, and the apple-shaped soil lumps are placed on the second base; 9, the old trees are transported to planting points for unloading, loading and planting.

The invention discloses a dipyridamole-loaded polyurethane anticoagulative material and a preparation process thereof. The material adopts a core-shell structure, the inner layer is a dipyridamole loaded layer, and the outer layer is a polyurethane main body layer. When the material is used as an artificial blood vessel to be transplanted, the dipyridamole can be slowly released in a long term, adhesion, aggregation and activation of platelets on the surface of the artificial blood vessel are inhibited, the blood compatibility of the artificial blood vessel is improved, the acute thrombosis and intima proliferation can be effectively avoided, and the transplanting success rate of the artificial blood vessel can be effectively improved.

The invention provides a bionic double-layer dressing, which comprises a decellularized animal small intestinal submucosa and a gel layer cross-linked on the film layer, and the film layer and the gel layer together form a bionic double-layer dressing with a double-layer structure; The preparation steps include preparation of decellularized small intestinal submucosa, preparation of decellularized small intestinal submucosa powder, preparation of decellularized small intestinal submucosa solution and preparation of bionic double-layer dressing. To the surface of the submucosa of the decellularized small intestine, the bionic double-layer dressing was obtained after freezing and cross-linking. The bionic double-layer dressing prepared by the invention not only has excellent tensile properties, but also has a semi-permeable barrier, which can prevent water evaporation and bacterial penetration. At the same time, the bionic double-layer dressing prepared by the invention is beneficial to the healing of the wound, can significantly increase the healing speed of the wound, and the wound healing rate is obviously accelerated on the 3rd day and the 7th day after the operation.

The invention discloses a fresh-keeping agent and application thereof in transportation and fresh keeping of tissue culture seedlings out of a culture medium. The fresh-keeping agent is prepared by taking the culture medium as a substrate and adding white sugar and an activity keeping agent into the substrate and mixing the raw materials, the fresh-keeping agent can be applied to the tissue culture seedlings out of the culture medium, nutrients required by plants and components for keeping plant activity are added and sprayed on seedlings out of the culture medium in a liquid fertilizer manner, so that on one hand, the nutrients required in the transportation process of the tissue culture seedlings are provided, on the other hand, the tissue culture seedlings can keep activity in the transportation process, meanwhile, the conditions of dead leaves, yellow leaves and weak growing power are greatly reduced, and the survival rate of the tissue culture seedlings is increased; and in addition, according to the scheme, the application method of the fresh-keeping agent is simple, operation is convenient, and the fresh-keeping agent can be suitable for industrial implementation.

A contact-type artificial anal sphincter prosthesis limit protection switch device, comprising: a motor casing, a transmission mechanism arranged in the motor casing, a swing arm mechanism, a limit mechanism and a controller, wherein: the swing arm mechanism and the transmission mechanism Connected, the limit mechanism includes: two limit switches connected to the controller respectively arranged on the swing arm mechanism and the motor casing, the first limit switch and the second limit switch are both switch round buttons and connected in series A limit switch circuit composed of a 10K resistor, the limit switch circuit leads to a high-level wire, a signal wire and a ground wire. In the present invention, by setting the limit switch, the switch round button can be pressed at the limit position where the swing arm of the lower ring swings to the left and right, respectively, to trigger the limit signal, and the limit signal generated by the limit switch will be transmitted to the controller for further processing. processing, simple structure, easy to realize, and high stability.

The invention discloses 2,7-substituted 3H-imidazole[4,5-b]pyridine compounds shown as the structural formula I, or pharmaceutically acceptable salt, ester and precursors, and / or hydrate and medicinal compositions thereof, and a preparation method for the compounds. The compounds, or pharmaceutically acceptable salt, ester and precursors, and / or hydrate and medicinal compositions thereof can serve as antagonists of CR4 and are used for treating or preventing CXCR4 receptor activation related diseases.

The invention belongs to the field of histocytology and tissue engineering and discloses a tissue-engineered epidermis with hypodermal cells and a preparation method thereof. The method for preparing the tissue-engineered epidermis with hypodermal cells comprises the following steps: obtaining epidermis cells; inoculating the obtained epidermis cells, culturing the cells until the density is 80-100 percent by using an epidermis culture medium, replacing the medium into a mixed culture medium, culturing for 16-24 hours, and collecting the epidermis cell layer, thereby obtaining the tissue-engineered epidermis piece; transferring the obtained tissue-engineered epidermis onto a semi-permeable polymer or a silicone mold substrate or a petrolatum gauze, thereby obtaining a film with the epidermis cell layer; and culturing the hypodermal cells, paving the obtained hypodermal cells on the epidermis cell layer with the film of the epidermis cell layer, and culturing for 1-2 hours, thereby obtaining the product. The preparation method provided by the invention is easy to operate and short in period, the tissue-engineered epidermis can be mutually stimulated and induced with receptor skin tissues, and formation of a complete epidermal structure of the receptor is promoted.