Tissue engineering skin and preparation method thereof

A tissue-engineered skin and dermis technology, applied in pharmaceutical formulations, pharmaceutical science, microcapsules, etc., can solve problems such as the inability to build skin

Active Publication Date: 2017-02-22
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a certain gap between tissue engineered skin and a truly ideal permanent skin substitute, especially the current technical methods cannot construct skin appendage structures in tissue engineered skin, such as hair follicles, sweat glands, etc.

Method used

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  • Tissue engineering skin and preparation method thereof
  • Tissue engineering skin and preparation method thereof
  • Tissue engineering skin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A preparation method of tissue engineered skin in this embodiment comprises the following steps:

[0072] Step 1: Preparation of medium

[0073] Mix DMEM / F12 (1:1) commercial culture medium with a volume ratio of 9:1 and fetal bovine serum as the basic culture medium; then add 2 ng of insulin, 10 μg of hydrocortisone, and basic fibroblast Cell growth factor 2 μg, transforming growth factor-β 5 μg, vascular endothelial growth factor 2 μg, recombinant human epidermal growth factor 2 μg, hepatocyte growth factor 5 μg, triiodothyronine 1 μg, sodium selenite 5 μg, penicillin 30 mg, streptomycin 30mg, 12mg of adenine, 1mg of transferrin, 5mg of vitamin C, 5mg of bovine pituitary extract, prepared as a culture medium for full-thickness tissue engineering skin.

[0074] Among them, basic fibroblast growth factor promotes the differentiation and proliferation of dermal cells, vascular endothelial growth factor promotes the differentiation and proliferation of vascular endotheli...

Embodiment 2

[0093] A preparation method of tissue engineered skin in this embodiment comprises the following steps:

[0094] Step 1: Preparation of medium

[0095] Mix DMEM / F12 (1:1) commercial culture medium with a volume ratio of 9:1 and fetal bovine serum as the basic culture medium; then add 25 ng of insulin and 300 μg of hydrocortisone according to the standard of 500 ml of base liquid, and basic fibroblasts Growth factor 25 μg, transforming growth factor-β 8 μg, vascular endothelial growth factor 10 μg, recombinant human epidermal growth factor 5 μg, hepatocyte growth factor 8 μg, triiodothyronine 1.5 μg, sodium selenite 8 μg, penicillin 40 mg, streptomycin 40mg, adenine 15mg, transferrin 5mg, vitamin C 15mg, bovine pituitary gland extract 8mg, prepared as culture medium for full-thickness tissue engineering skin.

[0096] Among them, basic fibroblast growth factor promotes the differentiation and proliferation of dermal cells, vascular endothelial growth factor promotes the differ...

Embodiment 3

[0112] A preparation method of tissue engineered skin in this embodiment comprises the following steps:

[0113] Step 1: Preparation of medium

[0114] Mix DMEM / F12 (1:1) commercial culture medium with a volume ratio of 9:1 and fetal bovine serum as the basic culture medium; then add 50 ng of insulin and 500 μg of hydrocortisone according to the standard of 500 ml of base liquid, and basic fibroblasts Growth factor 50 μg, transforming growth factor-β 10 μg, vascular endothelial growth factor 25 μg, recombinant human epidermal growth factor 10 μg, hepatocyte growth factor 10 μg, triiodothyronine 2 μg, sodium selenite 10 μg, penicillin 50 mg, streptomycin 50 mg , adenine 17mg, transferrin 10mg, vitamin C 30mg, bovine pituitary extract 10mg, prepared as a culture medium for full-thickness tissue engineering skin. Among them, basic fibroblast growth factor promotes the differentiation and proliferation of dermal cells, vascular endothelial growth factor promotes the differentiati...

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Abstract

The invention relates to tissue engineering skin and a preparation method thereof. The tissue engineering skin comprises a dermis layer and an epidermis layer, and is prepared from seed cells, cell growth factors and a biological scaffold material according to a biological 3D printing technology. The tissue engineering skin is similar to natural skin, comprises the dermis layer and the epidermis layer, can be produced in batches, can be closely attached to a wound, and has high biocompatibility, the dermis layer contains blood vessels, sweat glands and hair follicles, the shape, size and thickness of the tissue engineering skin can be easily changed, the elasticity and flexibility of the skin after sound healing can be enhanced, and the tissue engineering transplantation success rate can be increased.

Description

technical field [0001] The invention relates to the technical field of tissue engineering skin, in particular to a full-thickness tissue engineering skin containing appendages and a preparation method thereof. Background technique [0002] Tissue and organ defect or dysfunction is the main cause of harm to human health and one of the main causes of patient death. The repair and functional reconstruction of tissue and organ defect is a challenge in the medical field. Traditional treatment methods often require the sacrifice of autologous tissue for transplantation repair, which can easily lead to trauma and functional limitation of the donor site, which is a helpless move to "repair trauma with trauma". Extremely restrictive. With the development of medical science, the treatment concept of tissue and organ defects has gradually changed from tissue transplantation to tissue engineering. Tissue engineering refers to the combination of engineering principles and life sciences...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/54A61L27/60
CPCA61L27/3804A61L27/3808A61L27/3813A61L27/3834A61L27/54A61L27/60A61L2300/414A61L2300/622
Inventor 车七石钟经徳赵澎张聪冼钢梁超林刘少辉阵良艳
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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