23 results about "Hydrocortisone" patented technology

The invention provides a 3-hydroxypyrone derivative as well as a preparation method and application thereof, the compound can effectively inhibit release of proinflammatory cytokines in lipopolysaccharide-induced microglial cells, inhibit release of NO by the lipopolysaccharide-induced microglial cells, inhibit apoptosis of polysaccharide-induced microglial cells, and inhibit apoptosis of lipopolysaccharide-induced microglial cells. The compound has excellent anti-neuroinflammation activity and neuroprotective effect, the activity of the compound is superior to that of positive controls such as curcumin and hydrocortisone, and the compound can be used for preventing and / or treating neuroinflammation and neurodegenerative diseases.

The application relates to the technical field of animal model construction, in particular to a construction method and application of a spleen-kidney yang deficiency syndrome ovarian reserve function decline animal model. The construction method comprises the following steps: a model group animal is subjected to modeling by adopting the method of senna leaf water decoction gavage and hydrocortisone and cyclophosphamide injection, and a spleen-kidney yang deficiency syndrome ovarian reserve function decline animal model is obtained. In the application, the three of senna leaf, hydrocortisone and cyclophosphamide are used in synergy to administer the animal model, and the complex syndrome of the clinical spleen-kidney yang deficiency type DOR is accurately reproduced, and modern scientific basis is provided for the theories of traditional Chinese medicine such as the same treatment for different diseases.

Orodispersible film (ODF) is an innovative drug formulation that allows for adjustable dosing and improved patient compliance. It is administered into the mouth where it dissolves, making it suitable for children. The present invention provides an optimized ODF gel formulation and an optimal ODF containing therapeutic doses of hydrocortisone-type corticosteroids (HTC). This ODF is thin, flexible and transparent, and suitable for production in hospital pharmacy using standard equipment. The ODF of the invention allows accurate hydrocortisone levels to be released systemically in patients in need thereof, and its good physico-chemical stability is herein demonstrated for several months. This opens up new opportunities for use in pediatric patients, notably for children suffering from congenital adrenal hyperplasia.

This invention relates to a method for constructing and evaluating an animal model of Alzheimer's disease characterized by deficiency and blood stasis. Based on APP / PS1 mice, the model was induced by sleep deprivation, hydrocortisone, and carrageenan to induce qi deficiency, kidney deficiency, and blood stasis, respectively, for a total modeling period of 28 days, resulting in an animal model of Alzheimer's disease with deficiency and blood stasis. This model was constructed using a multi-factor combined approach, and its symptom manifestations are consistent with clinical observations. It is simple, effective, and easy to operate, providing a reliable experimental vehicle for the development of innovative drugs and the study of mechanisms of action in Alzheimer's disease with deficiency and blood stasis.

The present application relates to a culture method and culture medium for reducing the number of epithelial organoids and in vivo tissue differential transcripts, which solves the technical problems of low growth rate and low formation rate of organoids in the prior art. The epithelial organoid culture medium contains basic components and additional components. The basic components contain DMEM / F12 basic medium, penicillin-streptomycin solution, Glutamax additive, recombinant vertebral protein, recombinant cephalopod protein, Y-27632, epidermal growth factor, N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid buffer solution, nicotinamide, A83-01 and CHIR99021. The additional components contain Wnt-3A, ITS-X additive and hydrocortisone. The present application simultaneously provides a culture method for epithelial organoids. The present application can be used for the culture of epithelial organoids.

The invention discloses a hydrocortisone compound ointment preparation for treating haemorrhoids, and belongs to the technical field of medicines. The technical problem to be solved is to provide a hydrocortisone compound ointment preparation which has a good hemorrhoid treatment effect and is stable. The hydrocortisone compound ointment preparation comprises the following components in percentage by weight: 0.15%-1% of corticoid, 0.025%-0.1% of a vasoconstrictor, 2%-4% of a local anesthetic, 0.1%-1.5% of L-menthol, 0.02%-0.5% of chlorhexidine acetate and 0.05%-1% of allantoin. The hydrocortisone compound ointment preparation disclosed by the invention has the medicinal effects of relieving pain and discomfort of patients suffering from haemorrhoids, promoting healing and repairing of haemorrhoids tissues, preventing infection and relieving inflammatory response, and has obvious effects of stopping bleeding and relieving swelling and pain in haemorrhoids treatment.

The invention relates to the technical field of biology, in particular to a dissociation agent and a kit for progesterone determination. According to the invention, the sample dissociation agent for progesterone detection is obtained through optimized screening, and the sample dissociation agent comprises hydrocortisone, prednisolone and mono (2-ethylhexyl) phthalate (MEHP); when the dissociation agent components act independently or in a combined mode, the sensitivity to sample detection is high, the detection linear range is good, the dissociation agent is well related to a comparison reagent, the dissociation effect is improved remarkably on the whole, progesterone can be better released from corticosteroid binding protein, albumin and sex hormone binding globulin, and the progesterone dissociation agent is suitable for being used for detecting progesterone. The combination of progesterone and a captured antibody is facilitated, and the sensitivity and the accuracy can be effectively improved. Moreover, the reagent can be directly added into reagent components, so that the dissociation process and the immune reaction are carried out at the same time, the immune reaction is simple and efficient, and the reaction efficiency is improved.

The invention discloses a hydrocortisone butyrate cream and a preparation method thereof. Each gram of the hydrocortisone butyrate cream comprises the following components: 1 mg of hydrocortisone butyrate, 100-120 mg of an oil phase solid, 100-150 mg of white vaseline, 40-60 mg of light liquid paraffin, 30-40 mg of an emulsifier, 5-20 mg of salicylic acid, 10-40 mg of lecithin, 50-80 mg of a humectant, 1-2 mg of anhydrous sodium citrate, and the balance of water. On the basis of the existing prescription, the salicylic acid and the lecithin are added into the formula, and the dosages of the salicylic acid, the lecithin and other auxiliary materials are controlled, so that the hydrocortisone butyrate cream which is small in generation amount of degraded impurities and relatively good in stability is obtained.

The present disclosure relates generally to the treatment of adrenal dysfunction (such as congenital adrenal hyperplasia) with a combination of 2‑thiazolamine, 4-(2-chloro-4-methoxy-5-methylphenyl)-N-[(1S)-2-cyclopropyl-1-(3-fluoro-4-methylphenyl)ethyl]-5-methyl-N-2-propyn-1-yl (crinecerfont) and certain pharmaceutical compositions of hydrocortisone (such as a delayed release oral pharmaceutical composition of hydrocortisone).

This invention belongs to the field of regenerative medicine, specifically relating to a lacrimal gland organoid culture medium and a method for in vitro culture of lacrimal gland organoids. In one aspect, a lacrimal gland stem cell proliferation culture medium is provided, comprising: lacrimal gland stem cell basal culture medium, EGF, Wnt3a, FGF7, FGF10, RSP1, and Noggin; the lacrimal gland stem cell basal culture medium comprises: DMEM medium, F12 medium, L-glutamine, fetal bovine serum, hydrocortisone, cholera toxin, B27, penicillin, streptomycin, amphotericin B, and fasudil. In another aspect, a lacrimal gland stem cell differentiation culture medium is provided, comprising: lacrimal gland stem cell basal culture medium, EGF, Wnt3a, FGF7, FGF10, RSP1, Noggin, and BMP7. The lacrimal gland organoids constructed by this invention are larger in volume than those of existing technologies, possessing numerous branched ducts and vesicular acinar structures, extending radially and multidirectionally in a three-dimensional manner, forming a complex and regular three-dimensional tubular acinar network, and possessing secretory function.

The invention provides a method for producing porcine rotavirus based on suspension culture of a PK15 cell reactor, and belongs to the technical field of large-scale preparation of veterinary virus biological products. A Trans VA-06 special culture medium containing functional components such as hydrocortisone, IGF-1 and cholesterol is adopted, a PK15 cell high-density suspension system is constructed in a reactor, harvesting is conducted within 20-36 h when CPE is larger than or equal to 80% by accurately regulating and controlling the initial inoculation density, the culture volume, the stirring rotating speed and the ventilation / oxygen flux and cooperating with a virus inoculation strategy for fixing MOI, and it is shown through multiple batches of results that the PK15 cell high-density suspension system can be obtained. The titer of the obtained virus is stably 9.0 to 10.0 log10 TCID50 / mL, and the repeatability among batches is excellent; according to the method, the large-scale production efficiency and the product quality stability are improved, and the method is suitable for industrial production of the porcine rotavirus vaccine.

The invention belongs to the technical field of biology, and particularly relates to a culture medium capable of differentiating and inducing amniotic fluid cells into myoblasts, a method and application. The culture medium contains 5% v / v serum, 0.1 [mu] M of dexamethasone, 50 [mu] M of hydrocortisone, 1 [mu] M of MCHIR99021 and 5-10 ng / mL of IGF-1, and a solvent is a low-sugar DMEM (Dulbecco Modified Eagle Medium). According to the invention, amniotic fluid cells are separated from amniotic fluid, and myoblasts can be obtained by using the culture medium to culture, differentiate and induce. According to the invention, efficient muscle differentiation and RYR1 gene overexpression of amniotic fluid cells are realized for the first time, and the gene has high fidelity on splicing variation after induced differentiation, so that a safe, rapid and low-cost solution is provided for prenatal clear gene diagnosis.

Provided is a compound hydrocortisone and neomycin sulfate granule, including the following components in parts by mass: 140 parts of acetaminophen, 1 part of chlorpheniramine maleate, 1.2 parts of methylephedrine hydrochloride, 2 to 2.5 parts of an inclusion stabilizer, 3.0 parts of artificial calculus bovis, and 0.5 to 1 parts of a caramel. In a method for preparing the compound hydrocortisone and neomycin sulfate granule, natural Chinese medicinal herbs are subjected to extraction to obtain natural stabilizers for stabilizing low-concentration acetaminophen and chlorpheniramine maleate, and the natural stabilizers are encapsulated with hydroxypropyl-β-cyclodextrin with a specific degree of substitution (DS).

The present invention provides methods and pharmaceutical compositions for treating subjects with hepatic fibrosis and / or cirrhosis. [Solution] A method is provided for treating a subject with hepatic fibrosis, comprising administering a pharmaceutical composition containing hydronidone or a salt thereof to the subject. Liver stiffness measurements and / or ishax scores may be used to identify a subject for treatment of hepatic fibrosis and / or cirrhosis.

Formulations comprising hydrocortisone are disclosed in a suppository for methods of treating ulcerative colitis and other gastrointestinal diseases and disorders. Administering a single hydrocortisone suppository once a day achieves remission or reduction of a symptom, optionally followed by a period of time in which the dosage is reduced.

Use of hydronidone in the preparation of a medicament for preventing and / or treating chronic kidney disease.

This invention discloses a composite culture medium with defined components for co-culturing multiple cell types, along with its usage and applications. The culture medium can be used for long-term, stable triadic co-culture of patient-derived glioma stem cells, patient-derived T cells, and THP-1 macrophages (or patient-derived macrophages). The culture medium includes basal medium and EGF, bFGF, IL-2, M-CSF, ITS, and hydrocortisone. Furthermore, this invention provides optimized cell ratios and culture methods, achieving proliferation results that simultaneously maintain GSC stemness, primary T cell activity, and THP-1-derived macrophage activity for up to 14 days in the same system.

The disclosure provides a hemorrhoid splinting device intended for insertion into the anorectal cavity of a human. The device includes an elongated, fusiform body with a middle portion of larger diameter that tapers to smaller-diameter distal and proximal ends, where the distal end is rounded and the proximal end includes a handle. The device may feature surface recesses for administering medication, and / or an internal cavity for substances that retain coolant or heat. Another embodiment of the device includes an outer perforated skin layer for controlled release of therapeutic agents. Medications, such as hydrocortisone or witch hazel, may be applied to the recesses or positioned between the body and skin layer. Methods of treating hemorrhoids include inserting the device and maintaining the device inserted for a period of time.

To provide a method for producing a mature adipocyte-containing composition which has high revascularization ability and low possibility of inducing inflammation.SOLUTION: A method for producing mature adipocyte-containing composition includes: a cutting and filtering step of cutting and filtering an adipose tissue to which enzyme treatment is not performed to obtain micro-fat; a micro-fat culturing step of culturing micro-fat by using a culture medium after the cutting and filtering step to obtain a mature adipocyte; and a mature adipocyte-containing composition acquisition step of obtaining a mature adipocyte-containing composition containing the mature adipocyte and a conditioned medium obtained by subjecting micro-fat to the micro-fat culturing step, where the culture medium contains self serum or contains FBS, hydrocortisone and FGF-2.SELECTED DRAWING: Figure 1

The invention discloses an induction method of dopaminergic neural stem cells, and relates to the technical field of cell culture and neurobiology, and the induction method comprises the following steps: inoculating neural stem cells into an induction culture medium, culturing under the conditions of 37 DEG C and 5% CO2, and carrying out induced differentiation for 6-14 days to obtain dopaminergic neurons; the induction culture medium takes DMEM / F12 as a basic culture medium, and contains an effective amount of the following components: human insulin, progesterone, hydrocortisone, dexamethasone, estradiol, transferrin, an epidermal growth factor (EGF) and a fibroblast growth factor (FGF). The induction culture medium and the method have the advantages that the survival of neural stem cells can be effectively supported; and the gene is guided to directionally differentiate towards the dopaminergic neuron lineage. The method is clear in operation flow and stable in differentiation process, the expression of the characteristic marker of the dopaminergic neuron can be detected in the obtained cell population, and a stable and reliable cell source is provided for related scientific research.

Formulations comprising hydrocortisone are disclosed in a suppository for methods of treating ulcerative colitis and other gastrointestinal diseases and disorders. Administering a single hydrocortisone suppository once a day achieves remission or reduction of a symptom, optionally followed by a period of time in which the dosage is reduced.

Improved techniques for maintaining long-term cultures of the healthy and diseased human esophagus established in 2-dimension (2D) and 3-dimension is also needed. The invention disclosed herein generally relates to methods and systems for culturing and expanding esophageal cells with a growth media comprising a rho-kinase inhibitor, a WNT-activator / agonist, EGF, an inhibitor of TGF-BETA signaling, an inhibitor of BMP signaling, and hydrocortisone.

The invention discloses a construction and culture method of a sarcoma organ after nasopharyngeal carcinoma radiation. The sarcoma organ is prepared from basic components as follows: Advanced DMEM / F12, Ham's F-10 Nutrient Mix, fetal calf serum, GlutaMAX, penicillin-streptomycin (double antibodies), HEPES and N-acetylcysteine; the specific factor component is prepared from bFGF (basic fibroblast growth factor), FGF-10 (fibroblast growth factor), heparin, hEGF (human epidermal growth factor) and hIGF-2 (human insulin The additive comprises the following components: B27, N2 and A83-01 of which the vitamin A is removed, hydrocortisone, SB202190 and Y-27632; by using the culture medium, in-vitro nasopharynx cancer post-radiation sarcoma tissues can be efficiently constructed into nasopharynx cancer post-radiation sarcoma organs, and the obtained tumor organs are kept consistent with original tissues in the aspects of tissue pathological structures and tumor heterogeneity.