12 results about "Hydrocortisone" patented technology

The application relates to the technical field of animal model construction, in particular to a construction method and application of a spleen-kidney yang deficiency syndrome ovarian reserve function decline animal model. The construction method comprises the following steps: a model group animal is subjected to modeling by adopting the method of senna leaf water decoction gavage and hydrocortisone and cyclophosphamide injection, and a spleen-kidney yang deficiency syndrome ovarian reserve function decline animal model is obtained. In the application, the three of senna leaf, hydrocortisone and cyclophosphamide are used in synergy to administer the animal model, and the complex syndrome of the clinical spleen-kidney yang deficiency type DOR is accurately reproduced, and modern scientific basis is provided for the theories of traditional Chinese medicine such as the same treatment for different diseases.

Orodispersible film (ODF) is an innovative drug formulation that allows for adjustable dosing and improved patient compliance. It is administered into the mouth where it dissolves, making it suitable for children. The present invention provides an optimized ODF gel formulation and an optimal ODF containing therapeutic doses of hydrocortisone-type corticosteroids (HTC). This ODF is thin, flexible and transparent, and suitable for production in hospital pharmacy using standard equipment. The ODF of the invention allows accurate hydrocortisone levels to be released systemically in patients in need thereof, and its good physico-chemical stability is herein demonstrated for several months. This opens up new opportunities for use in pediatric patients, notably for children suffering from congenital adrenal hyperplasia.

The invention relates to the technical field of biology, in particular to a dissociation agent and a kit for progesterone determination. According to the invention, the sample dissociation agent for progesterone detection is obtained through optimized screening, and the sample dissociation agent comprises hydrocortisone, prednisolone and mono (2-ethylhexyl) phthalate (MEHP); when the dissociation agent components act independently or in a combined mode, the sensitivity to sample detection is high, the detection linear range is good, the dissociation agent is well related to a comparison reagent, the dissociation effect is improved remarkably on the whole, progesterone can be better released from corticosteroid binding protein, albumin and sex hormone binding globulin, and the progesterone dissociation agent is suitable for being used for detecting progesterone. The combination of progesterone and a captured antibody is facilitated, and the sensitivity and the accuracy can be effectively improved. Moreover, the reagent can be directly added into reagent components, so that the dissociation process and the immune reaction are carried out at the same time, the immune reaction is simple and efficient, and the reaction efficiency is improved.

The invention discloses a hydrocortisone butyrate cream and a preparation method thereof. Each gram of the hydrocortisone butyrate cream comprises the following components: 1 mg of hydrocortisone butyrate, 100-120 mg of an oil phase solid, 100-150 mg of white vaseline, 40-60 mg of light liquid paraffin, 30-40 mg of an emulsifier, 5-20 mg of salicylic acid, 10-40 mg of lecithin, 50-80 mg of a humectant, 1-2 mg of anhydrous sodium citrate, and the balance of water. On the basis of the existing prescription, the salicylic acid and the lecithin are added into the formula, and the dosages of the salicylic acid, the lecithin and other auxiliary materials are controlled, so that the hydrocortisone butyrate cream which is small in generation amount of degraded impurities and relatively good in stability is obtained.

This invention belongs to the field of regenerative medicine, specifically relating to a lacrimal gland organoid culture medium and a method for in vitro culture of lacrimal gland organoids. In one aspect, a lacrimal gland stem cell proliferation culture medium is provided, comprising: lacrimal gland stem cell basal culture medium, EGF, Wnt3a, FGF7, FGF10, RSP1, and Noggin; the lacrimal gland stem cell basal culture medium comprises: DMEM medium, F12 medium, L-glutamine, fetal bovine serum, hydrocortisone, cholera toxin, B27, penicillin, streptomycin, amphotericin B, and fasudil. In another aspect, a lacrimal gland stem cell differentiation culture medium is provided, comprising: lacrimal gland stem cell basal culture medium, EGF, Wnt3a, FGF7, FGF10, RSP1, Noggin, and BMP7. The lacrimal gland organoids constructed by this invention are larger in volume than those of existing technologies, possessing numerous branched ducts and vesicular acinar structures, extending radially and multidirectionally in a three-dimensional manner, forming a complex and regular three-dimensional tubular acinar network, and possessing secretory function.

The invention belongs to the technical field of biology, and particularly relates to a culture medium capable of differentiating and inducing amniotic fluid cells into myoblasts, a method and application. The culture medium contains 5% v / v serum, 0.1 [mu] M of dexamethasone, 50 [mu] M of hydrocortisone, 1 [mu] M of MCHIR99021 and 5-10 ng / mL of IGF-1, and a solvent is a low-sugar DMEM (Dulbecco Modified Eagle Medium). According to the invention, amniotic fluid cells are separated from amniotic fluid, and myoblasts can be obtained by using the culture medium to culture, differentiate and induce. According to the invention, efficient muscle differentiation and RYR1 gene overexpression of amniotic fluid cells are realized for the first time, and the gene has high fidelity on splicing variation after induced differentiation, so that a safe, rapid and low-cost solution is provided for prenatal clear gene diagnosis.

The present invention provides methods and pharmaceutical compositions for treating subjects with hepatic fibrosis and / or cirrhosis. [Solution] A method is provided for treating a subject with hepatic fibrosis, comprising administering a pharmaceutical composition containing hydronidone or a salt thereof to the subject. Liver stiffness measurements and / or ishax scores may be used to identify a subject for treatment of hepatic fibrosis and / or cirrhosis.

Formulations comprising hydrocortisone are disclosed in a suppository for methods of treating ulcerative colitis and other gastrointestinal diseases and disorders. Administering a single hydrocortisone suppository once a day achieves remission or reduction of a symptom, optionally followed by a period of time in which the dosage is reduced.

Use of hydronidone in the preparation of a medicament for preventing and / or treating chronic kidney disease.

This invention discloses a composite culture medium with defined components for co-culturing multiple cell types, along with its usage and applications. The culture medium can be used for long-term, stable triadic co-culture of patient-derived glioma stem cells, patient-derived T cells, and THP-1 macrophages (or patient-derived macrophages). The culture medium includes basal medium and EGF, bFGF, IL-2, M-CSF, ITS, and hydrocortisone. Furthermore, this invention provides optimized cell ratios and culture methods, achieving proliferation results that simultaneously maintain GSC stemness, primary T cell activity, and THP-1-derived macrophage activity for up to 14 days in the same system.

The disclosure provides a hemorrhoid splinting device intended for insertion into the anorectal cavity of a human. The device includes an elongated, fusiform body with a middle portion of larger diameter that tapers to smaller-diameter distal and proximal ends, where the distal end is rounded and the proximal end includes a handle. The device may feature surface recesses for administering medication, and / or an internal cavity for substances that retain coolant or heat. Another embodiment of the device includes an outer perforated skin layer for controlled release of therapeutic agents. Medications, such as hydrocortisone or witch hazel, may be applied to the recesses or positioned between the body and skin layer. Methods of treating hemorrhoids include inserting the device and maintaining the device inserted for a period of time.

Formulations comprising hydrocortisone are disclosed in a suppository for methods of treating ulcerative colitis and other gastrointestinal diseases and disorders. Administering a single hydrocortisone suppository once a day achieves remission or reduction of a symptom, optionally followed by a period of time in which the dosage is reduced.