76 results about "Cyclophosphamide" patented technology

This invention belongs to the fields of biomedicine and cosmetics, and discloses the application of holly glycoside in promoting hair growth and preventing hair loss. Holly glycoside promotes the transition of hair follicles from the telogen phase to the anagen phase (i.e., shortening the telogen phase and prolonging the anagen phase), and inhibits cyclophosphamide-induced apoptosis of hair follicle cells and DHT-induced miniaturization of hair follicles. In androgenetic alopecia, telogen effluvium, and chemical alopecia models, its hair growth-promoting effect is comparable to that of the positive control drug minoxidil. It has broad application prospects in pharmaceutical formulations, cosmetics, skincare products, and beauty products.

This invention provides a method for preparing simian cyclophosphamide, comprising the following steps: loading simian cyclophosphamide extract into a macroporous adsorption resin column, performing first column chromatography using a first eluent to obtain simian cyclophosphamide component; loading the simian cyclophosphamide component into a reversed-phase silica gel column, performing second column chromatography using a second eluent to obtain crude simian cyclophosphamide; and loading the crude simian cyclophosphamide into a gel chromatography column, performing third column chromatography using a third eluent to obtain purified product. This preparation method utilizes a combination of macroporous adsorption resin column, reversed-phase silica gel column, and gel chromatography for purification, along with a specific elution procedure and eluent. The resulting simian cyclophosphamide I has a purity of over 99%, meeting the purity requirements for detection reference standards (>98%) and content determination reference standards (>99%), with an extraction yield of over 50%.

The invention belongs to the technical field of medicines, and particularly relates to a pharmaceutical composition for improving ovarian function damage and degeneration and a preparation method thereof. The invention discloses the GnRH agonist effect of dihydromyricetin DMY for the first time, and the affinity of dihydromyricetin DMY to a GnRH receptor and the GnRH agonist effect of dihydromyricetin DMY are verified through in-vitro experiments. Model animal experiments further prove that the dihydromyricetin DMY has a protective effect on ovarian function impairment caused by chemotherapy. Experiments prove that the dihydromyricetin DMY is given while the cyclophosphamide (CTX) is used for chemotherapy, so that the damage of the dihydromyricetin DMY to the ovarian function during chemotherapy can be reduced, and a protection effect is also achieved on the recovery of the ovarian function after drug withdrawal. The protection effect is reflected on the endocrine function and the reserve function of the ovary, and the preserved ovary reserve function can achieve the pregnancy result after chemotherapy is finished, which indicates that the dihydromyricetin DMY also plays the protection effect on the fertility function of the ovary.

The invention relates to a preparation method and a verification method of deer skin collagen peptide with skin repairing and immunoregulation effects, and belongs to the technical field of biological medicines. The technical problems that the technology for extracting the deer skin collagen peptide from the deer skin of the sika deer is tedious, and whether the prepared deer skin collagen peptide has skin repairing and immunoregulation effects or not are solved. The preparation method of the deer skin collagen peptide with the skin repairing and immunoregulation effects comprises the following steps: cutting deer skin into small pieces, and preparing the deer skin collagen peptide with the molecular weight of less than 10kDa by adopting a double-step enzymolysis method. And a verification experiment of an immunosuppressive mouse is constructed through cyclophosphamide induction. According to the invention, the extraction process is greatly simplified, and a double-step enzymolysis method is adopted, so that the deer skin collagen peptide subjected to enzymolysis is small-molecule peptide, has strong antioxidant capacity, can repair light-damaged cells and promote skin cell growth, and is good in safety; by injecting the deer skin collagen peptide, the immune function of the mouse is improved, and damage caused by mouse cells is relieved.

The invention relates to the technical field of biological medicines, in particular to a maggot alcohol extract for improving chemotherapy immune injury, a preparation method of the maggot alcohol extract and application of the maggot alcohol extract in preparation of medicines, health-care products or functional foods for preventing or treating immune injury caused by chemotherapy medicines. It is found for the first time that the maggot alcohol extract has a remarkable protection effect on immune injury caused by chemotherapeutic drugs such as cyclophosphamide, the number of leukocytes and lymphocytes can be effectively increased, and immune organ atrophy is relieved. The maggot alcohol extract disclosed by the invention can be used for preparing medicines, health care products or functional foods for preventing or treating immune injury caused by chemotherapy, has the characteristics of multiple target spots, high efficiency and low toxicity, is simple and stable in preparation process, and has good development and application prospects.

The present invention relates, in general terms, to a method for treating autoimmune diseases with immunotherapy. The present invention also relates, in general terms, to a method for treating autoimmune diseases comprising administering a cyclophosphamide preconditioning regimen and subsequently administering immunotherapy. TIFF2026514435000017.tif68165

The application discloses a tumor full-nutrition functional emulsion, which comprises the following components in mass: 10-14% of protein, 7-10% of fat, 12-16% of carbohydrate, 0.01-0.02% of vitamin, 0.5-1.0% of mineral substance and 0.5-1% of food additive, and the balance is water; the protein comprises oyster low-molecular peptide and whey protein at a mass ratio of 2-3:8-12; and the food additive comprises Tween 80 and polyglycerol ricinoleate at a mass ratio of 70-75:20-23. The tumor full-nutrition functional emulsion has a water-in-oil-in-water structure and has freeze-thaw stability; after nutrition support of the tumor full-nutrition functional emulsion, the body's immune regulation ability can be significantly enhanced, the side effect of cyclophosphamide chemotherapy can be reduced, and the body's malnutrition and immunosuppression can be effectively improved.

The invention discloses application of exosome-like nanoparticles derived from fructus lycii to preparation of a medicine for preventing or treating oligospermia and / or asthenospermia. Sterile, stable and high-purity fructus lycii exosome-like nanoparticles (LBELNs) are obtained by adopting a differential centrifugation-suction filtration-ultra-high-speed centrifugation method, the physiological efficacy of the LBELNs is researched, and it is found that the LBELNs can significantly improve the oligoasthenospermia disease activity index of male mice induced by cyclophosphamide (CTX); the sperm quantity is obviously increased, the sperm malformation is improved, the sperm motility is improved, and the sex hormone level is recovered. The LBELNs can effectively relieve spermatogenic dysfunction of testicular tissues, the sperm differentiation and formation process is obviously improved, the total number of sperms is increased, and the treatment effect is more obvious along with increase of the dosage of the LBELNs. The invention has potential in preparation of drugs for preventing or treating oligoasthenospermia, and provides a new strategy and treatment method for treating oligoasthenospermia.

The invention discloses application of a caenorhabditis elegans reproductive disorder model to screening of strains promoting reproduction health, and relates to the technical field of biologica.Cyclophosphamide medicine intervention is applied to caenorhabditis elegans, so that the normal reproductive system of caenorhabditis elegans is damaged, and the reproductive disorder model is prepared; then 3985 gene knockout strains in an escherichia coli Keio gene knockout library are utilized, and strains with the reproductive capacity remarkably improved for reproductive disorder are screened; and carrying out homologous gene knockout on the probiotics by using a gene editing technology, and screening out the probiotics with the potential of promoting reproductive health. The reproductive disorder model is stable and repeatable, can be used for screening strains or drugs for improving the reproductive function, and also can be used for screening candidate strains or drugs for reducing the reproductive toxicity of cyclophosphamide. According to the method, an effective tool is provided for screening of reproduction-related probiotic strains or screening of related drugs, and meanwhile, the strains screened based on the model provide new candidate strains for treatment and research of reproductive disorder diseases.

The invention belongs to the field of biological pharmacy, and particularly relates to a chalcanthicidin compound and application thereof. The invention discloses a chalcanthicidin compound which is separated from a secondary metabolite of marine actinomycetes Streptomyces sp. KCB-132 (marine actinomycetes sp. KCB-132). Pharmacological activity experiments show that the compound has a moderate inhibition effect on ESKAPE pathogenic bacteria with multiple drug resistance, such as enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii and escherichia coli. Therefore, the compound has the potential of being developed into a novel antibacterial agent.

The invention belongs to the technical field of biological medicines, and particularly relates to application of an SIRT5 inhibitor MC3482 in preparation of a medicine for treating premature ovarian failure. According to the invention, a mouse POI model is constructed through the induction of cyclophosphamide (CTX), and the MC3482 is proved to be capable of inhibiting the activity of SIRT5, reducing excessive macrophage infiltration of ovary caused by CTX, reducing M1 type macrophage activation and proinflammatory factor release, further inhibiting the apoptosis of ovarian granular cells, protecting follicular reserve and recovering ovarian functions. The invention clarifies the action effect and application scheme of MC3482 for preventing and treating POI, provides a new treatment strategy for chemotherapy-induced iatrogenic POI, and has important clinical transformation value.

The invention relates to the technical field of biological medicines, and discloses an application of a plant extract composition in preparation of a medicine for improving DNA damage of testicular spermatogenic cells, and the plant extract contains a herba epimedii extract and a rhizoma polygonati extract. The invention provides new application of the composition containing the herba epimedii extract and the rhizoma polygonati extract in preparation of the medicine for improving DNA damage of the testicular spermatogenic cells, the composition can remarkably improve the DNA damage of the testicular spermatogenic cells of mice caused by cyclophosphamide, recover the multiplication capacity of the mice, inhibit apoptosis of the mice and relieve spermatogenic function damage caused by cyclophosphamide, and the composition can be used for preparing the medicine for improving the DNA damage of the testicular spermatogenic cells of the mice. And the combined administration shows a synergistic effect instead of a simple additive effect, and the drug effect is obviously superior to that of any one of the epimedium extract and the rhizoma polygonati extract which are independently administered.

This invention discloses a plant-derived bactericidal composition and its application in fruit juice sterilization. The bactericidal composition consists of two plant-derived compounds with a synergistic effect, including phloretin and dodecylaldehyde, wherein the mass ratio of dodecylaldehyde to phloretin is (1-10):1. At room temperature and pressure, adding the bactericidal composition directly to fruit juice and maintaining it for 2 days can kill the vegetative cells and spores of *Bacillus cyclophosphamide*. This invention is non-toxic, harmless, simple, convenient, green, energy-saving, has high sterilization efficiency, and has no adverse effects on the nutrients and flavor of the fruit juice. This invention is highly effective in solving the problem of spoilage and deterioration of acidic fruit juice beverages caused by incomplete sterilization and spore killing.

This invention discloses the application of *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder in the preparation of immunomodulatory drugs, belonging to the field of bio-fermentation and immunomodulation technology. The *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder is prepared by fermenting ginseng with *Lactobacillus rhamnosus*. The rare ginsenosides include at least one of Ck, Rk1, Rh4, and Rg5. The immunomodulatory drug is used to improve cyclophosphamide-induced immunodeficiency. The amount of *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder added to the immunomodulatory drug is 10%-50% (mass fraction). The preparation method of the *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder is as follows: S1, ginseng pretreatment; S2, strain activation; S3, fermentation culture; S4, freeze-dried powder preparation. This invention has the technical effects of high bioavailability of ginseng active ingredients, significant immunomodulatory effect, good safety, and effective improvement of cyclophosphamide-induced immunodeficiency.

The application belongs to the field of new adjuvants and new dosage forms of pharmaceutical preparations, and particularly relates to a type I and type II immunogenic cell death inducer ion pairing engineered nanoassemblies as well as construction and application thereof. The nanoassemblies are composed of type I ICD inducers, type II ICD inducers, hydrophobic auxiliary counterions and amphiphilic lipid ion pairing. The type I ICD inducer is selected from mitoxantrone, doxorubicin, oxaliplatin or cyclophosphamide; the type II ICD inducer is selected from hypericin; and the hydrophobic auxiliary counterion is selected from cholesteryl sodium sulfate, cholic acid, deoxycholic acid, chenodeoxycholic acid, lithocholic acid, glycolcholic acid, taurocholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, deoxycholic acid lysine derivative, oleanolic acid and ursolic acid. The type I and type II ICD inducers are combined in the application, and have a synergistic tumor treatment effect.

The invention discloses a wolfberry-astragalus compound polysaccharide with an anti-myelosuppression effect as well as a preparation method and application of the wolfberry-astragalus compound polysaccharide. The wolfberry-astragalus compound polysaccharide is prepared by adopting a process of separately extracting wolfberry polysaccharide and astragalus polysaccharide and then mixing, the total sugar content is 70-80%, the uronic acid content is 10-14%, and the wolfberry-astragalus compound polysaccharide has specific molecular weight distribution and monosaccharide composition characteristics. Animal experiment results show that the wolfberry and astragalus compound polysaccharide can significantly improve peripheral hemopenia of myelosuppression mice induced by cyclophosphamide, promote recovery of the number of leukocytes, lymphocytes, monocytes and neutrophils, and effectively reverse weight loss caused by chemotherapeutic drugs. The wolfberry-astragalus compound polysaccharide provided by the invention is high in safety and remarkable in anti-myelosuppression effect, and can be used for preparing medicines or functional preparations for improving myelosuppression related diseases.

The invention discloses application of tremella aurantialba polysaccharide in preparation of a medicine for treating male immunological dyszoospermia, and belongs to the field of biological medicine. The invention provides a new application of tremella aurantialba polysaccharide, and experiments prove that the tremella aurantialba polysaccharide can improve dyszoospermia induced by cyclophosphamide, significantly increase the number of sperms of mice, reduce the abnormal rate of sperms and promote secretion of testosterone. According to the invention, cyclophosphamide is adopted to construct a male mouse dyszoospermia model, tremella aurantialba polysaccharides extracted in different manners are subjected to intragastric administration, the action mechanism of NAPs for improving mouse dyszoospermia is researched, the mechanism of NAPs for treating spermatogenesis is disclosed, and a basis is provided for clinical application.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

The invention relates to a preparation method of a hippophae rhamnoides leaf isorhamnetin glycoside enriched solid beverage, and belongs to the field of functional plant beverage processing. The preparation method comprises the following steps: preparing sea buckthorn green tea and sea buckthorn green tea freeze-dried powder by taking sea buckthorn leaves as a main raw material; carrying out ethanol extraction and AB-8 type macroporous resin separation and purification to obtain an isorhamnetin glycoside substance enriched component; a Maillard composite system formed by whey protein isolate and maltodextrin is loaded with an isorhamnetin glycoside substance enrichment component, the isorhamnetin glycoside substance enrichment component is compounded with sea buckthorn green tea freeze-dried powder, freeze drying is performed, and the isorhamnetin glycoside enrichment solid beverage is obtained. The isorhamnetin glycoside enriched solid beverage disclosed by the invention has good solubility, and shows a remarkable immunological enhancement effect in a cyclophosphamide induced immunocompromised mouse model. The method has a wide application prospect in the aspects of high-value utilization of hippophae rhamnoides leaf resources and functional beverage development.

The invention relates to the technical field of traditional Chinese medicines, in particular to application of a prepared rehmannia root extract in preparation of a medicine for treating ovarian injury. The inventor finds a single traditional Chinese medicine for treating ovarian injury based on a traditional Chinese medicine compound core medicinal material data mining model. The prepared rehmannia root is taken as a representative core medicinal material screened and output by a model, efficacy verification is carried out in a cyclophosphamide induced ovarian injury mouse model, and the result shows that the prepared rehmannia root can improve E2 and AMH levels, reduce FSH and LH levels, improve orbital cycle disorder and reduce oxidative stress level, so that the prepared rehmannia root can improve the ovarian reserve function of a mouse, and can be used for treating ovarian injury. And a certain improvement effect is achieved on ovarian injury. The invention provides a quantifiable traditional Chinese medicine compound medicinal material screening thought, realizes a closed loop of a method from data mining to efficacy verification, and provides a theoretical basis and an experimental basis for developing novel traditional Chinese medicines for treating ovarian injury.

The invention relates to the technical field of pharmaceutical preparations, and discloses a doxorubicin and cyclophosphamide liposome compound injection which is prepared from doxorubicin hydrochloride, cyclophosphamide, hydrogenated soybean phosphatidylcholine, cholesterol, polyethylene glycol derivative phospholipid, anhydrous magnesium sulfate, L-arginine and a sucrose octasulfate ammonium solution. Magnesium ions introduced into an inner water phase participate in competitive binding, doxorubicin and sucrose octasulfate are induced to form a loose co-precipitation structure with lattice defects, hydrophilic cyclophosphamide is physically filled and cured by using micro gaps, and efficient encapsulation of non-gradient dependent drugs is realized. Meanwhile, an in-situ pH buffer system is constructed by using L-arginine, protons generated in a drug loading process are neutralized, an internal water phase is maintained in a neutral environment, and acid-catalyzed hydrolysis of cyclophosphamide is effectively inhibited. According to the invention, the double-drug encapsulation efficiency and storage stability of the compound liposome are obviously improved.

The present application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition, a preparation method and application thereof. Specifically, based on the theory of traditional Chinese veterinary medicine of'strengthening the body resistance and eliminating pathogenic factors, and treating the root and branch', aiming at the pathogenesis of 'deficiency in the root and excess in the branch' of low immunity in animals, eight kinds of medicinal materials are scientifically screened to form a traditional Chinese medicine compound with the effects of tonifying qi and nourishing blood, invigorating the spleen and kidney, dredging collaterals and eliminating dampness, and clearing heat and detoxifying. Through the verification of a cyclophosphamide-induced low immunity mouse model, the traditional Chinese medicine composition can significantly improve the mental state of the mice, restore the body weight and thymus / spleen index, repair the pathological damage of the spleen tissue, and adjust the inflammatory factor level in the serum and spleen tissue. In addition, in a Marek's disease virus infection model, the traditional Chinese medicine composition combined with the Marek's disease virus vaccine can significantly reduce the viral load in the peripheral blood, liver and spleen, and reduce the liver enlargement, hemorrhage and microscopic structure damage, showing a good immune synergistic effect.

The application discloses a method for establishing a Bama miniature pig spleen deficiency and dampness excess model, and belongs to the technical field of animal models. The method is to reduce the immunity of the body by intraperitoneal injection of cyclophosphamide, and then to stimulate the Bama miniature pig by using high-sugar and high-fat feed and senna leaves in combination with a high-humidity external environment. After 8 weeks, the Bama miniature pig has the clinical manifestations of the spleen deficiency and dampness excess, such as body fatness, fatigue and laziness, white and slippery tongue fur, and loose and watery stool, and the model is verified in the reverse direction by using the classic compound Shenglin Bai Zhusan. The Bama miniature pig spleen deficiency and dampness excess model is applied to the research of human spleen deficiency and dampness excess and the development of spleen-invigorating and dampness-expelling drugs. The model provides a new model for standardizing, standardizing and scientifically evaluating the research of spleen-invigorating and dampness-expelling traditional Chinese medicines, and has the advantages of good practicability, strong conformity, simplicity and objectivity, and conforms to the guidance of the theory of traditional Chinese medicine syndromes, and has a wide application prospect.

The invention discloses an osmanthus fragrans extract for preventing premature ovarian failure and a preparation method and application thereof.The extract is prepared from osmanthus fragrans through microwave fixation, gradient temperature weak acid extraction, membrane separation and concentration, low-temperature drying and other pure physical processes, effective components can be prevented from being damaged, the process is stable, green and efficient, the active components are definite, and the preparation method is simple. Wherein the sum of the contents of the salidroside, the verbascoside and the ligustroside is not less than 20%. Animal experiments show that AMH, FSH and LH hormone levels in mice suffering from premature ovarian failure induced by cyclophosphamide can be remarkably improved, the ovarian function can be effectively improved, all index components including salidroside, verbascoside and ligustrum glycoside have a synergistic interaction effect, a safe and effective solution scheme derived from natural plants is provided for preventing premature ovarian failure, and the application prospect is wide. The method can be widely applied to preparation of various products.

The invention discloses jute homogeneous polysaccharide with an immune activation effect as well as a preparation method and application thereof, and belongs to the technical field of medicines. The invention aims to solve the technical problems of improving immune organ atrophy, caused by cyclophosphamide, of an immunosuppression model mouse, recovering morphological characteristics and blood cell quantity of the spleen and promoting generation of immune and inflammatory cytokines, so that the immune function of the mouse is recovered, and the effect of immune activation is achieved. The technical scheme is characterized in that the jute homogeneous polysaccharide is formed by connecting 3-4 parts by mole of rhamnose, 0.5-2 parts by mole of arabinose, 1-3 parts by mole of galactose and 1-2 parts by mole of galacturonic acid through glucosidic bonds.

The invention discloses Chinese wolfberry fruit composite puree, a preparation method thereof and application of the Chinese wolfberry fruit composite puree in preparation of a preparation for relieving cyclophosphamide toxicity, and relates to the technical field of traditional Chinese medicine combination medicines. Fresh Chinese wolfberry fruits and Chinese wolfberry fruit seed oil are used as main components of the Chinese wolfberry fruit composite puree. Experimental results show that the Chinese wolfberry fruit composite raw stock has an effect of relieving toxicity caused by cyclophosphamide, has a better curative effect than single use of Chinese wolfberry fruits, can effectively enhance the muscle strength of mice, enables aspartate transaminase (AST) and glutamic-pyruvic transaminase (ALT) with abnormally increased vitality to return to normal, resists abnormal swelling of the liver, relieves hepatotoxicity, improves the defense function of the body, and has a good application prospect. Therefore, the mouse death condition caused by cyclophosphamide poisoning is improved. The Chinese wolfberry fruit puree belongs to a health-care product, has the advantages of definite components and safety, and provides a new strategy and means for intervention and treatment of side effects caused by chemotherapy for tumor patients.

The invention provides a preparation method of ifosfamide, an intermediate and an ifosfamide composition, and belongs to the technical field of medicines. Comprising the following steps: S1, carrying out cyclization reaction on phosphorus oxychloride and n-propanolamine under the action of an acid-binding agent to obtain a compound 1; s2, the compound 1 and 3-halo-propane-1-alcohol are subjected to a reaction, and a compound 2 is prepared; s3, reacting the compound 2 with ethanolamine to prepare a compound 3; and S4, carrying out a reaction on the compound 3 and thionyl chloride to prepare the ifosfamide. The preparation method of the ifosfamide is simple, mild in reaction condition, obviously improved in total yield, fewer in by-products and higher in product purity, and has a wide application prospect.

The invention discloses an in-vitro rapid detection method for ovarian repair activity of mesenchymal stem cell source exosomes. Specifically, the invention provides a KGN granular cell damage model based on 4-hydroxycyclophosphamide (4-hydroxycyclophosphamide, 4HCy) induction, and the model can be used for constructing an exosome function quality control system which is rapid, quantifiable, dose-dependent, capable of defining a threshold value and suitable for batch quality control. And a unified standard is provided for the processes of preparation, identification, function detection, quality control and release of the extracellular vesicles.

The present invention relates generally to methods of treating autoimmune diseases with immune cell therapy. The present invention also relates generally to methods of treating autoimmune diseases comprising performing a pre-conditioning regimen with cyclophosphamide, and subsequently performing immune cell therapy.

The invention relates to the technical field of polyurethane, and discloses a water-based polyurethane fireproof coating and a preparation method thereof, pentaerythritol diphosphate diphosphoryl chloride, 4-methoxy-L-phenylalanine, pyridine hydrochloride and the like are used as reactants to prepare a novel carboxyl-containing water-based flame-retardant chain extender, and the water-based flame-retardant chain extender is used as a fire retardant. And carrying out polymerization reaction with dihydric alcohol and diisocyanate monomer to obtain the waterborne polyurethane fireproof coating. The polyurethane water-based emulsion is good in dispersity, high in emulsion stability and excellent in hardness; meanwhile, a nitrogen-phosphorus flame-retardant structure of the bis-spirophosphamide is grafted into a polyurethane molecular chain, so that the combustion limit oxygen index of the polyurethane film material is remarkably improved and reaches 27.9-29.6%, and the UL-94 grade reaches V-0.