11 results about "Cyclophosphamide" patented technology

This invention belongs to the fields of biomedicine and cosmetics, and discloses the application of holly glycoside in promoting hair growth and preventing hair loss. Holly glycoside promotes the transition of hair follicles from the telogen phase to the anagen phase (i.e., shortening the telogen phase and prolonging the anagen phase), and inhibits cyclophosphamide-induced apoptosis of hair follicle cells and DHT-induced miniaturization of hair follicles. In androgenetic alopecia, telogen effluvium, and chemical alopecia models, its hair growth-promoting effect is comparable to that of the positive control drug minoxidil. It has broad application prospects in pharmaceutical formulations, cosmetics, skincare products, and beauty products.

This invention provides a method for preparing simian cyclophosphamide, comprising the following steps: loading simian cyclophosphamide extract into a macroporous adsorption resin column, performing first column chromatography using a first eluent to obtain simian cyclophosphamide component; loading the simian cyclophosphamide component into a reversed-phase silica gel column, performing second column chromatography using a second eluent to obtain crude simian cyclophosphamide; and loading the crude simian cyclophosphamide into a gel chromatography column, performing third column chromatography using a third eluent to obtain purified product. This preparation method utilizes a combination of macroporous adsorption resin column, reversed-phase silica gel column, and gel chromatography for purification, along with a specific elution procedure and eluent. The resulting simian cyclophosphamide I has a purity of over 99%, meeting the purity requirements for detection reference standards (>98%) and content determination reference standards (>99%), with an extraction yield of over 50%.

This invention discloses the application of *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder in the preparation of immunomodulatory drugs, belonging to the field of bio-fermentation and immunomodulation technology. The *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder is prepared by fermenting ginseng with *Lactobacillus rhamnosus*. The rare ginsenosides include at least one of Ck, Rk1, Rh4, and Rg5. The immunomodulatory drug is used to improve cyclophosphamide-induced immunodeficiency. The amount of *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder added to the immunomodulatory drug is 10%-50% (mass fraction). The preparation method of the *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder is as follows: S1, ginseng pretreatment; S2, strain activation; S3, fermentation culture; S4, freeze-dried powder preparation. This invention has the technical effects of high bioavailability of ginseng active ingredients, significant immunomodulatory effect, good safety, and effective improvement of cyclophosphamide-induced immunodeficiency.

The application belongs to the field of new adjuvants and new dosage forms of pharmaceutical preparations, and particularly relates to a type I and type II immunogenic cell death inducer ion pairing engineered nanoassemblies as well as construction and application thereof. The nanoassemblies are composed of type I ICD inducers, type II ICD inducers, hydrophobic auxiliary counterions and amphiphilic lipid ion pairing. The type I ICD inducer is selected from mitoxantrone, doxorubicin, oxaliplatin or cyclophosphamide; the type II ICD inducer is selected from hypericin; and the hydrophobic auxiliary counterion is selected from cholesteryl sodium sulfate, cholic acid, deoxycholic acid, chenodeoxycholic acid, lithocholic acid, glycolcholic acid, taurocholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, deoxycholic acid lysine derivative, oleanolic acid and ursolic acid. The type I and type II ICD inducers are combined in the application, and have a synergistic tumor treatment effect.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

The present application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition, a preparation method and application thereof. Specifically, based on the theory of traditional Chinese veterinary medicine of'strengthening the body resistance and eliminating pathogenic factors, and treating the root and branch', aiming at the pathogenesis of 'deficiency in the root and excess in the branch' of low immunity in animals, eight kinds of medicinal materials are scientifically screened to form a traditional Chinese medicine compound with the effects of tonifying qi and nourishing blood, invigorating the spleen and kidney, dredging collaterals and eliminating dampness, and clearing heat and detoxifying. Through the verification of a cyclophosphamide-induced low immunity mouse model, the traditional Chinese medicine composition can significantly improve the mental state of the mice, restore the body weight and thymus / spleen index, repair the pathological damage of the spleen tissue, and adjust the inflammatory factor level in the serum and spleen tissue. In addition, in a Marek's disease virus infection model, the traditional Chinese medicine composition combined with the Marek's disease virus vaccine can significantly reduce the viral load in the peripheral blood, liver and spleen, and reduce the liver enlargement, hemorrhage and microscopic structure damage, showing a good immune synergistic effect.

The application discloses a method for establishing a Bama miniature pig spleen deficiency and dampness excess model, and belongs to the technical field of animal models. The method is to reduce the immunity of the body by intraperitoneal injection of cyclophosphamide, and then to stimulate the Bama miniature pig by using high-sugar and high-fat feed and senna leaves in combination with a high-humidity external environment. After 8 weeks, the Bama miniature pig has the clinical manifestations of the spleen deficiency and dampness excess, such as body fatness, fatigue and laziness, white and slippery tongue fur, and loose and watery stool, and the model is verified in the reverse direction by using the classic compound Shenglin Bai Zhusan. The Bama miniature pig spleen deficiency and dampness excess model is applied to the research of human spleen deficiency and dampness excess and the development of spleen-invigorating and dampness-expelling drugs. The model provides a new model for standardizing, standardizing and scientifically evaluating the research of spleen-invigorating and dampness-expelling traditional Chinese medicines, and has the advantages of good practicability, strong conformity, simplicity and objectivity, and conforms to the guidance of the theory of traditional Chinese medicine syndromes, and has a wide application prospect.

This invention relates to the application of Cordyceps militaris polysaccharide in improving the adverse reactions of cyclophosphamide (CPA), belonging to the field of biomedical technology. The Cordyceps militaris polysaccharide product of this invention exhibits good therapeutic effects in improving CPA adverse reactions, providing a superior candidate substance for alleviating chemotherapy side effects. This invention actively guides Cordyceps militaris to synthesize a structurally optimized and activity-enhanced Cordyceps militaris polysaccharide CM-T2 through a unique induction method. In vivo experimental data show that, compared with the extracellular polysaccharide CM-C2 produced by Cordyceps militaris under uninduced conditions, CM-T2 exhibits a more significant protective effect against CPA-induced immunosuppression and hepatobiliary damage at the same dose. The Cordyceps militaris polysaccharide of this invention provides an adjunctive treatment option for reducing the side effects of chemotherapy drugs such as CPA, aiming to alleviate the suffering caused by chemotherapy and improve patients' survival rate and quality of life.

This invention discloses a polysaccharide extracted from *Gynostemma pentaphyllum* using ultrasound-assisted extraction, its preparation method, and its applications, belonging to the field of biomedical technology. This polysaccharide significantly improves peripheral blood counts in mice with cyclophosphamide-induced anemia, alleviates immune organ atrophy and liver damage, and its mechanism of action involves regulating the intestinal flora structure and modulating host sphingolipid and glycerophospholipid metabolic pathways. The preparation method efficiently yields homogeneous polysaccharides from *Gynostemma pentaphyllum*, with optimized process conditions of a liquid-to-solid ratio of 32 mL / g, extraction time of 48 min, and temperature of 60 ℃. The process of this invention is stable and provides a new candidate substance for developing drugs or health foods for the prevention and treatment of chemotherapy-related anemia.

The invention discloses an active peptide capable of directionally inducing IPSCS cracking, a preparation method of the active peptide and application of the active peptide in a preparation for repairing liver injury. Experiments prove that the active peptide provided by the invention has a remarkable protective effect on a liver injury model induced by cyclophosphamide and shows a definite anti-oxidative stress effect. The result shows that a key path of chemotherapy injury can be intervened from the source by simulating or enhancing an endogenous protection mechanism of the liver. Compared with the existing exogenous synthetic drugs or single-component natural products, the product provided by the invention has the potential advantages of more comprehensive action mechanism and better biogenic property. Meanwhile, the extract is derived from directionally differentiated functional cells, and a brand new thought and material basis are provided for developing a novel chemotherapy liver protective agent which does not affect the chemotherapy curative effect and has good biocompatibility.

This invention belongs to the field of cosmetic preparation technology, specifically relating to a composition with anti-aging and whitening effects and its preparation method. The composition comprises 8-10 parts by weight of cyclophosphamide skin extract, 1-2 parts of lemon extract, and 0.25-1.25 parts of pullulan. Cyclophosphamide skin extract is rich in small-molecule marine collagen active peptides and various skin-friendly amino acids, which can reduce collagen fiber degradation and loss, and improve early signs of aging such as skin laxity and fine lines. Lemon extract is rich in natural, highly active polyphenols, flavonoids, and vitamins, exhibiting outstanding antioxidant capacity. It can quickly remove excess free radicals from the skin, alleviate oxidative stress damage, and improve dullness and pigmentation. Pullulan, as a natural water-soluble high-molecular-weight moisturizing film-forming matrix raw material, can form a thin, breathable protective film on the skin surface, effectively locking in moisture. Therefore, the above composition can be used for anti-aging and whitening.