44 results about "Reproductive toxicity" patented technology

The invention discloses a construction method of a zebra fish reproductive toxicity model, a method for evaluating reproductive toxicity of chemical substances and application, and relates to the technical field of reproductive development toxicity of chemical substances. According to the method for evaluating the reproductive toxicity of the chemical substance provided by the invention, the change of a diatrix behavior can be concerned by shooting behavioral pictures or videos without sacrifice of an experimental animal or professional fluorescence imaging instrument equipment and tissue slicing equipment; the method is more in line with animal ethics, cost is saved, and continuous observation can be realized by using the same batch of organisms. The test courtship behavior can quickly utilize software to analyze and obtain zebra fish behavior changes. By adopting the evaluation method provided by the invention, both female ovarian malformation and male reproductive toxicity can be detected, and the evaluation method has the advantage of more evaluation dimensions. Based on the method provided by the invention, a zebrafish reproductive toxicity model can be constructed, and the model can be used as a drug screening platform for reproductive function damage of zebrafish.

The invention relates to the technical field of biological detection, and discloses a caenorhabditis elegans reproductive toxicity intelligent evaluation system and an application method.The caenorhabditis elegans reproductive toxicity intelligent evaluation system comprises a standardized exposure and imaging module used for executing a high-flux pollutant exposure experiment under a controlled environment condition and synchronously collecting nematode behavior and gonad image data; and the multi-modal data acquisition unit is connected to the standardized exposure and imaging module and is used for extracting and preprocessing behavioral parameters and reproductive toxicity indexes from the acquired images. According to the method, 12 behavior parameters and 8 core reproductive indexes are automatically extracted by constructing a multi-modal data acquisition unit and utilizing an improved U-Net model and a specific algorithm, so that full-process automation from image acquisition to key index quantification is realized. The problems of low efficiency, large subjective deviation and poor repeatability caused by dependence on manual interpretation in the prior art are solved, and the objectivity of the evaluation result and the high-throughput screening efficiency are remarkably improved.

The invention discloses a multi-endpoint reproductive toxicity prediction method and system based on a large language model, and belongs to the technical field of toxicity prediction. The method comprises the following steps: extracting phenotype information of text data in a candidate data set by using the large language model; performing multi-dimensional annotation processing on the phenotype level annotation data set; inputting the reproductive toxicity training set into a pre-constructed molecular representation learning model for training, and constructing a reproductive toxicity prediction model set with phenotype specificity; performing performance evaluation on the plurality of classifiers in the reproductive toxicity prediction model set by using the test data set, and screening according to preset performance indexes to obtain an optimal prediction model combination; performing toxicity prediction on the molecular structure of the chemical substance to be detected by using the optimal prediction model combination to obtain a multi-dimensional reproductive toxicity prediction result including cross-gender, cross-generation and cross-research types; according to the method, high-resolution and multi-dimensional reproductive toxicity phenotype prediction is carried out on the environmental chemical substances.

The present invention discloses the application of trans-anethole in alleviating the toxicity of zearalenone in animals, belonging to the technical field of mycotoxin prevention and control. The present invention provides the application of trans-anethole in the preparation of a drug or feed for alleviating zearalenone poisoning in animals. The present invention has confirmed that trans-anethole can effectively alleviate the reproductive toxicity of zearalenone in piglets, improve the uterine organ index and ovarian organ index, ensure the health of piglets and the development of the reproductive system, and provide a new method for detoxifying zearalenone.

The invention discloses application of combination of ginsenoside Rb1 and luteolin in prevention and treatment of male reproductive toxicity caused by organophosphorus pesticides, and belongs to the field of medicines. A high-toxicity organophosphorus pesticide glyphosate is used as a representative, the reproductive toxicity effect of the glyphosate on male mice and the relieving effect of the ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin on the male reproductive toxicity of the glyphosate are observed through tests, and the result shows that the ginsenoside Rb1 and the luteolin composition have the effect of inhibiting the reproductive toxicity of the glyphosate. The ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin have a remarkable relieving effect on male reproductive toxic diseases caused by organophosphorus pesticides, wherein the male reproductive toxic diseases comprise testis diseases and sperm malformation rate increase, and antioxidant index GSH decrease and T-SOD increase caused by the organophosphorus pesticides. Effective drugs and treatment strategies are provided for preventing and treating organophosphorus pesticide induced male reproductive toxicity and diseases induced by organophosphorus pesticide induced male reproductive toxicity.

The invention relates to palmitoyl tripeptide-38 and a preparation method and application thereof.According to the palmitoyl tripeptide-38, low-toxicity and biodegradable NFM and anisole are compounded according to the volume ratio of 1: 1, and the palmitoyl tripeptide-38 has strong polarity and moderate hydrophobicity and can completely replace DMF. According to the mixed solvent, the solubility of Fmoc amino acid monomers, activating reagents and resin is improved, the risk of reproductive toxicity is avoided, and the volatility is reduced compared with that of DMF (Dimethyl Formamide); the process is synergistically enhanced, namely, the mass transfer limitation of the traditional intermittent reaction is overcome through the synergism of the laminar shear effect and the ultrasonic cavitation effect of a continuous flow reaction device, so that the coupling reaction time of the palmitoyl tripeptide-38 is shortened, the occurrence rate of side reaction is reduced, and the universality verifies that the solvent system has excellent performance in synthesis of the palmitoyl tripeptide-38, and the solvent system is suitable for industrial production. And the coupling efficiency is stable.

The invention discloses application of a caenorhabditis elegans reproductive disorder model to screening of strains promoting reproduction health, and relates to the technical field of biologica.Cyclophosphamide medicine intervention is applied to caenorhabditis elegans, so that the normal reproductive system of caenorhabditis elegans is damaged, and the reproductive disorder model is prepared; then 3985 gene knockout strains in an escherichia coli Keio gene knockout library are utilized, and strains with the reproductive capacity remarkably improved for reproductive disorder are screened; and carrying out homologous gene knockout on the probiotics by using a gene editing technology, and screening out the probiotics with the potential of promoting reproductive health. The reproductive disorder model is stable and repeatable, can be used for screening strains or drugs for improving the reproductive function, and also can be used for screening candidate strains or drugs for reducing the reproductive toxicity of cyclophosphamide. According to the method, an effective tool is provided for screening of reproduction-related probiotic strains or screening of related drugs, and meanwhile, the strains screened based on the model provide new candidate strains for treatment and research of reproductive disorder diseases.

The invention discloses an application of triptophenol terpene in preparation of a medicine for treating diabetic nephropathy, belongs to the technical field of medicines, is obtained by separating and purifying tripterygium glycosides extract, and is applied as a single active component or a core component in DKD treatment medicines, and solves the problems that a traditional tripterygium glycosides preparation is high in toxicity and uncontrollable in curative effect. The medicine of the triptophenol terpene can obviously inhibit kidney inflammatory factors (IL-6 and TNF-alpha) and fibrosis markers (TGF-beta); the urine protein level is reduced, and the kidney function is improved; and hepatotoxicity and reproductive toxicity risks are avoided.

The invention relates to the field of medicines, and discloses application of triptophenol terpene in preparation of a medicine for treating rheumatoid arthritis. According to the application disclosed by the invention, the effect of treating rheumatoid arthritis of the triptophenol terpene is found for the first time, and obvious hepatorenal toxicity and reproductive toxicity are not observed under an effective in-vivo dosage, so that the triptophenol terpene has a higher clinical application value.

The invention discloses an application of butyric acid or a derivative thereof in preparation of a zearalenone reproductive toxicity resisting preparation. Also disclosed is an antitoxic feed composition comprising a specific base ration and a butyric acid derivative. In-vivo and in-vitro tests and molecular mechanism researches prove that butyric acid and derivatives thereof (such as tributyrin and sodium butyrate) can effectively relieve zearalenone (ZEN)-induced animal ovary injury. The action mechanism of the traditional Chinese medicine composition is that follicular atresia is reduced, the balance of reproductive hormones (such as GnRH, E2, AMH and P4) is recovered, and clinical symptoms caused by ZEN poisoning such as vulva swelling and the like are relieved by activating a BMPs / SMAD signal channel, especially up-regulating SMAD4 protein expression and inhibiting oxidative stress (ROS) and Caspase-3 mediated granular cell apoptosis.

The invention relates to the technical field of traditional Chinese medicines, in particular to a pharmaceutical composition for treating autoimmune diseases or kidney diseases and tripterygium glycosides tablets containing the pharmaceutical composition. The pharmaceutical composition disclosed by the invention is prepared from five active ingredients, namely triptolide, triptophenolide, neotriptophenolide, triptochloro lactone and triptophenolide. On the basis of cell membrane chromatography (CMC) and mass spectrometry technologies, active ingredients, having a specific binding effect on glomerular podocytes, in the tripterygium glycosides tablets are screened and identified, and the toxicity is reduced and the curative effect is improved by optimizing the formula ratio. In-vitro cell experiments and animal model verification are combined, and a novel tripterygium glycosides tablet formula strategy is provided. Research results show that the optimized composition significantly reduces hepatotoxicity and reproductive toxicity while retaining core anti-inflammatory and anti-fibrosis activity, and provides a safer solution for clinical application of the tripterygium wilfordii preparation.

The invention discloses an application of 3 '-sialic acid lactose in preparation of a medicine for treating Alzheimer's disease. The invention further discloses application of the 3 '-sialic acid lactose or the medicinal salt thereof in preparation of a medicine for inhibiting Tau protein aggregation, and a medicinal composition containing the 3'-sialic acid lactose. The specific sialic acid derivative can effectively block pathological aggregation of tau, and the treatment effect of the medicine on AD is remarkably improved. The 3, 3 '-sialic acid lactose is low in acute toxicity, has no genetic toxicity and reproductive toxicity after being taken for a long time at a high dosage, and has relatively good medicinal safety and relatively good medicinal prospects.

Provided is a 3, 5, 5-trimethylhexanoic acid composition which contains 3, 5, 5-trimethylhexanoic acid and tertiary butanol, and which has a deuterated toluene-converted concentration of more than 0 to 400 volume ppb (inclusive) as measured by smelling gas chromatography / mass spectrometry after heating in an air atmosphere at 80 DEG C for 4 weeks. The composition has low reproductive toxicity even if stored for a long period of time. (Measurement Condition). The 3, 5, 5-trimethylhexanoic acid composition is concentrated. And measuring the concentrated sample by smelling gas chromatography using a column in which the stationary phase is dimethyl polysiloxane, and analyzing the structure of the measured components by a mass spectrometry detector.

This invention discloses the application of cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins in the preparation of drugs for treating or preventing aging and lowering lipids. In vivo experiments have confirmed that the cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins of this invention, without reproductive toxicity to a *C. elegans* model, can prolong the average lifespan of nematodes, improve their motility, enhance their stress resistance, reduce endogenous ROS levels and lipofuscin content, and decrease the body fat content of nematodes. Therefore, the cinnamyl flavanone alkaloids and phenylpropanol-substituted ester-type catechins of this invention can be used in the preparation of drugs for treating or preventing aging and lowering lipids.

The invention relates to application of Chinese yam protein, in particular to application of Chinese yam active protein in preparation of medicine for regulating blood testis barrier to treat testis dysfunction. L-YP has unique fluorescence characteristics and remarkable antioxidant activity, and can effectively improve testis immune microenvironment disorder by repairing blood testis barriers and inhibiting NLRP3 and TLR4 / MyD88 / NF-kappa B inflammatory pathways. In combination with a molecular docking technology, a molecular mechanism that key components such as YP2 (mitochondrial ornithine aminotransferase) and the like realize immunoregulation through high-affinity binding is clarified for the first time. According to the application disclosed by the invention, a molecular docking technology is creatively combined with system experimental analysis, and a molecular mechanism of improving testis injury by the Chinese yam protein is illuminated from the perspective of a'structure-function-target 'synergistic effect, so that a novel dietary intervention strategy is provided for preventing reproductive toxicity caused by chemotherapy; the application potential of medicinal and edible components in the field of male reproductive health is highlighted.

The invention relates to the technical field of environmental medicine and reproductive toxicology, in particular to application of aspirin in prevention and / or treatment of male reproductive injury caused by rare earth elements. The invention also relates to a method for improving sperm quality reduction caused by rare earth element exposure. The method comprises the step of administering an effective dose of aspirin or a pharmaceutically acceptable salt thereof to an individual in need thereof. The invention also relates to a pharmaceutical composition for preventing and / or treating male reproductive injury caused by rare earth elements. The cognitive limitation of a traditional oxidative stress mechanism is broken through, a new mechanism of rare earth reproductive toxicity is disclosed from a new perspective of testis immune microenvironment for the first time, and an intervention scheme aiming at the new mechanism is provided. According to the invention, the aspirin is creatively used for solving the novel public health problem of low-dose long-term exposure of rare earth elements for the first time, and a new application field is developed for non-steroidal anti-inflammatory drugs.

The invention provides a harmful outcome path-based chemical reproductive toxicity transfer learning model prediction method, which comprises the following steps of: collecting test data of male and female reproductive toxicity of chemicals in different levels in a harmful outcome path, and preprocessing the test data; randomly splitting the preprocessed test data into a training set, a verification set and a test set, training a source model by using a deep neural network, and obtaining a target model after migration; evaluating the prediction performance of the target model by taking the area under the curve, the F1 score and the balance accuracy as indexes; representing an application domain of the target model according to the index similarity density and the weighted rugged property; and predicting whether the chemicals in the application domain have reproductive toxicity to the living body or not by using the target model. The transfer learning model fully fuses multi-level in-vitro test toxicity data of chemicals from molecular initial events to harmful outcomes, and can accurately predict in-vivo reproductive toxicity.

The present invention belongs to the technical field of natural medicine chemistry, and specifically relates to an effective part of Menispermi Rhizoma extract, a preparation method thereof, and an application thereof in the preparation of a drug for preventing or treating polycystic ovary syndrome (PCOS). The effective part of the Menispermi Rhizoma extract is mainly a mixture of daurisoline (DAU) and methyl daurisolate (DAU-Me), and in terms of mass percentage, the proportion is 75%-95%. The effective part of the Menispermi Rhizoma extract provided by the present invention significantly improves the estrous cycle disorder, insulin resistance, blood sex hormone disorder and ovarian polycystic-like changes of PCOS model mice, and does not cause obvious reproductive toxicity, and can be used for the preparation of a drug for preventing or treating polycystic ovary syndrome.

The invention relates to the technical field of medicines, and discloses an application of lupin or an extract thereof in preparation of a drug for relieving reproductive toxicity of chemotherapeutic drugs, the lupin is selected from lupin plants, preferably lupin variety Jenabillup, and the chemotherapeutic drugs comprise busulfan, cyclophosphamide or cis-platinum. The administration dosage of the lupin or the extract thereof is 0.1-100 mg / kg body weight, the administration period is continuous administration for at least 5 weeks, experiments show that the lupin can significantly improve the sperm concentration and sperm motility of chemotherapy model animals and improve pathological damage of testicular tissues, the mechanism relates to antioxidant, anti-inflammatory and anti-apoptosis effects, and the lupin or the extract thereof can be used for preparing a chemotherapy model animal. The invention provides a safe and effective new strategy for fertility protection of chemotherapy patients.

The invention discloses an application of a tetrahydroindazole derivative in preparation of drugs for resisting reproduction toxicity of bisphenol compounds, the tetrahydroindazole derivative is specifically N-(4-(3-fluorophenoxy) phenyl)-N '-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide, the molecular formula is C16H17F3N2O3, and the structural formula is shown in the formula (I). By investigating the influence of N-(4-(3-fluorophenoxy) phenyl)-N '-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide on the egg laying amount of caenorhabditis elegans, the obtained experimental result shows that N-(4-(3-fluorophenoxy) phenyl)-N'-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide has the effect of resisting reproductive toxicity of bisphenol compounds, and can be used for preparing the caenorhabditis elegans egg laying agent. The total egg laying amount of the nematodes after bisphenol S exposure can be remarkably increased to be close to the level of a control group, so that the method is expected to be applied to preparation of various bisphenol compound reproductive toxicity resisting drugs.

The invention particularly discloses a medicine for treating lupus nephritis and application thereof, and relates to the technical field of biological medicine. According to the application of the extracellular vesicles and the tripterygium wilfordii in preparation of the medicine for treating lupus nephritis, the mesenchymal stem cell apoptosis vesicles and the tripterygium wilfordii are combined for treatment, the immunoregulation, anti-inflammation and other characteristics of the tripterygium wilfordii and the mesenchymal stem cell apoptosis vesicles are combined, the dosage of the tripterygium wilfordii can be reduced, the toxic and side effects of the tripterygium wilfordii can be greatly reduced, and the curative effect of the tripterygium wilfordii is improved. , such as reproductive system toxicity, gastrointestinal reactions, and liver and kidney function impairment.

The invention relates to the technical field of environmental medicine and reproductive toxicology, in particular to application of aspirin in prevention and / or treatment of male reproductive injury caused by rare earth elements. The invention also relates to a method for improving sperm quality reduction caused by rare earth element exposure. The method comprises the step of administering an effective dose of aspirin or a pharmaceutically acceptable salt thereof to an individual in need thereof. The invention also relates to a pharmaceutical composition for preventing and / or treating male reproductive injury caused by rare earth elements. The cognitive limitation of a traditional oxidative stress mechanism is broken through, a new mechanism of rare earth reproductive toxicity is disclosed from a new perspective of testis immune microenvironment for the first time, and an intervention scheme aiming at the new mechanism is provided. According to the invention, the aspirin is creatively used for solving the novel public health problem of low-dose long-term exposure of rare earth elements for the first time, and a new application field is developed for non-steroidal anti-inflammatory drugs.

The application belongs to the technical field of reproductive health, and particularly relates to a new application of a testicular metabolite 2-n-propylthiazolidine-4-carboxylic acid (PTCA), and the 2-n-propylthiazolidine-4-carboxylic acid is used for preparing a drug for treating testicular spermatogenic disorder induced by AFB1. The PTCA can promote the secretion of testosterone of testicular interstitial cells, activate the PI3K / AKT / NRF2 antioxidant signaling pathway in testicular tissue, relieve testicular injury induced by AFB1, and reduce the ROS level in testicular tissue. The application discloses, for the first time, the important role of the PTCA as a new testicular metabolic marker and protective agent in the male reproductive system, provides a new insight into the reproductive toxicity mechanism of AFB1, and lays an experimental foundation for the application of metabolites to the intervention strategy of male spermatogenic disorder.

The application discloses a biomarker for evaluating bisphenol S male reproductive toxicity and application thereof, and belongs to the technical field of biological medicine. The biomarker for evaluating bisphenol S male reproductive toxicity is screened out based on multi-omics integration and functional phenotype, and the biomarker comprises one or more of a Cyp2e1 gene, a Tdo2 gene and a Ugt1a1 gene. The expression amount of the above biomarker is detected by a detection reagent to evaluate the male reproductive toxicity of bisphenol S, and the detection reagent has high accuracy and specificity. A corresponding detection kit and a use method are developed, and the male reproductive toxicity of bisphenol S can be quickly and accurately evaluated.

The present application relates to the technical field of breeding, in particular to an indoor breeding of the formula feed of the chemi-smithia brycei and the breeding method of the chemi-smithia brycei, the formula feed is added with the compound essential oil of eugenol, citral and cinnamic acid (mass ratio 0.8:0.5:0.3), the formula feed is low in mouldy rate and zero in formaldehyde residue. The breeding method comprises the following steps: directly feeding the fermented feed to the newly hatched larvae (without the transition of rice stems), feeding the low-density 3rd instar larvae in different pipes (10 larvae per dish), and replacing the feed only once in the whole process. The application of the present application completely eliminates the reproductive toxicity of chemical preservatives and is suitable for large-scale year-round breeding of standard insect sources.

The invention discloses application of lycium barbarum red in treating male reproductive toxicity, and belongs to the technical field of biological medicine. The invention provides application of lycium barbarum red in preparation of a medicine for treating male reproductive toxicity. The male reproductive toxicity is caused by exposure to benzophenone substances or exposure to diethylstilbestrol. Animal experiments find that the lycium barbarum red can effectively improve testis function damage induced by benzophenone substance BP-3 or estrogen diethylstilbestrol, improve seminiferous tubule damage degree, increase seminiferous epithelium height, increase sperm number, spermatogonium and primary spermatoblasts, improve spermatogenic ability, increase pregnancy rate, improve sperm yield, improve sperm quality and improve sperm quality. The growth and development of offspring are promoted.

The application discloses a method for evaluating the reproductive toxicity of microplastics, comprising the following steps: (1) providing a FXR1 protein phase separation report system, which is selected from an in vitro phase separation system or an intracellular report system; (2) contacting a microplastic sample to be tested with the FXR1 protein phase separation report system; (3) observing and detecting the influence of the microplastic sample to be tested on the FXR1 protein phase separation phenomenon in the report system by using fluorescence microscopic imaging technology, wherein the influence includes the morphological change of FXR1 protein phase separation droplets and / or the flowability change of FXR1 protein phase separation droplets; (4) evaluating the reproductive toxicity risk level of the microplastic sample to be tested based on the detection result of step (3).

An external traditional Chinese medicine composition for treating hyperemesis gravidarum, comprising the following active pharmaceutical ingredients: 250-300 g of ginger processed Pinellia, 250-300 g of dried tangerine peel, 250-300 g of clove, 250-300 g of medicated leaven, and 300-400 ml of ginger juice. The external traditional Chinese medicine composition is used for preparing a traditional Chinese medicine acupoint application to treat hyperemesis gravidarum. The therapeutic method using the external traditional Chinese medicine application is safe for the maternal body and fetus, and has little reproductive toxicity. External therapy is used to enliven the spleen and whet the appetite, which can promote an increase in the appetite of a patient suffering from hyperemesis gravidarum. By means of acupoint application, the traditional Chinese medicine composition acts on a Neiguan acupoint (the Jueyin Pericardium Meridian of Hand) and a Zusanli acupoint (the Stomach Meridian of Foot-Yangming), thereby calming the adverse-rising energy and controlling nausea and vomiting, and decreasing the frequency of vomiting. The traditional Chinese medicine external application therapy, with intravenous fluid supportive therapy, can significantly relieve vomiting symptoms and shorten the length of stay and the time of therapy.

This application provides a novel tabletop ventilation device, relating to the field of reproductive toxicity testing technology for non-clinical safety evaluation. It includes a hood and an exhaust duct. The hood is generally polyhedral, with an openable and closable door on at least one side. One end of the exhaust duct is connected to at least one hood, and the other end is connected to an exhaust system for discharging air from inside the hood to the outside of the laboratory. The hood is placed on a tabletop within the laboratory. The internal space of the hood is used to accommodate a microscope and specimens. The hood has an observation window, and the eyepiece of the microscope can be aligned with the observation window. By covering the microscope with the hood, the internal space of the hood is relatively sealed, which can confine the formaldehyde gas volatilized from formalin to a controllable area. When specimens are placed inside the hood for operation, the external exhaust system can provide power to promptly expel the air containing formaldehyde and other pollutants from the hood, effectively reducing the occupational exposure risks of respiratory damage and skin allergies for laboratory personnel.

The invention relates to the technical field of plant and edible microorganism product separation, and relates to a toadstool phenolic acid glycoside extract and a preparation method and application thereof.The extraction method of the toadstool glycoside extract comprises the following steps that S1, toadstool is dried, smashed, sieved and degreased, and toadstool powder is obtained; s2, adding a solvent into the morchella esculenta powder, and performing ultrasonic treatment, filtration and concentration to obtain a crude extract; s3, deproteinizing the crude extract, passing through macroporous resin, and freeze-drying to obtain the toadstool glycoside extract, the ultrasonic power is 200-400 W, the ultrasonic temperature is 40-56 DEG C, and the ultrasonic time is 20-46 min. According to the method, the extraction rate of toadstool glycoside is remarkably increased by optimizing parameters. The toadstool glycoside extract disclosed by the invention can be used for increasing the nematode hatching rate, the total egg laying number and the brood number, inhibiting germ cell apoptosis and remarkably relieving anti-reproductive toxicity.