26 results about "Reproductive toxicity" patented technology

The invention relates to the technical field of biological detection, and discloses a caenorhabditis elegans reproductive toxicity intelligent evaluation system and an application method.The caenorhabditis elegans reproductive toxicity intelligent evaluation system comprises a standardized exposure and imaging module used for executing a high-flux pollutant exposure experiment under a controlled environment condition and synchronously collecting nematode behavior and gonad image data; and the multi-modal data acquisition unit is connected to the standardized exposure and imaging module and is used for extracting and preprocessing behavioral parameters and reproductive toxicity indexes from the acquired images. According to the method, 12 behavior parameters and 8 core reproductive indexes are automatically extracted by constructing a multi-modal data acquisition unit and utilizing an improved U-Net model and a specific algorithm, so that full-process automation from image acquisition to key index quantification is realized. The problems of low efficiency, large subjective deviation and poor repeatability caused by dependence on manual interpretation in the prior art are solved, and the objectivity of the evaluation result and the high-throughput screening efficiency are remarkably improved.

The invention discloses a multi-endpoint reproductive toxicity prediction method and system based on a large language model, and belongs to the technical field of toxicity prediction. The method comprises the following steps: extracting phenotype information of text data in a candidate data set by using the large language model; performing multi-dimensional annotation processing on the phenotype level annotation data set; inputting the reproductive toxicity training set into a pre-constructed molecular representation learning model for training, and constructing a reproductive toxicity prediction model set with phenotype specificity; performing performance evaluation on the plurality of classifiers in the reproductive toxicity prediction model set by using the test data set, and screening according to preset performance indexes to obtain an optimal prediction model combination; performing toxicity prediction on the molecular structure of the chemical substance to be detected by using the optimal prediction model combination to obtain a multi-dimensional reproductive toxicity prediction result including cross-gender, cross-generation and cross-research types; according to the method, high-resolution and multi-dimensional reproductive toxicity phenotype prediction is carried out on the environmental chemical substances.

The invention discloses application of combination of ginsenoside Rb1 and luteolin in prevention and treatment of male reproductive toxicity caused by organophosphorus pesticides, and belongs to the field of medicines. A high-toxicity organophosphorus pesticide glyphosate is used as a representative, the reproductive toxicity effect of the glyphosate on male mice and the relieving effect of the ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin on the male reproductive toxicity of the glyphosate are observed through tests, and the result shows that the ginsenoside Rb1 and the luteolin composition have the effect of inhibiting the reproductive toxicity of the glyphosate. The ginsenoside Rb1 and the composition of the ginsenoside Rb1 and the luteolin have a remarkable relieving effect on male reproductive toxic diseases caused by organophosphorus pesticides, wherein the male reproductive toxic diseases comprise testis diseases and sperm malformation rate increase, and antioxidant index GSH decrease and T-SOD increase caused by the organophosphorus pesticides. Effective drugs and treatment strategies are provided for preventing and treating organophosphorus pesticide induced male reproductive toxicity and diseases induced by organophosphorus pesticide induced male reproductive toxicity.

The invention relates to palmitoyl tripeptide-38 and a preparation method and application thereof.According to the palmitoyl tripeptide-38, low-toxicity and biodegradable NFM and anisole are compounded according to the volume ratio of 1: 1, and the palmitoyl tripeptide-38 has strong polarity and moderate hydrophobicity and can completely replace DMF. According to the mixed solvent, the solubility of Fmoc amino acid monomers, activating reagents and resin is improved, the risk of reproductive toxicity is avoided, and the volatility is reduced compared with that of DMF (Dimethyl Formamide); the process is synergistically enhanced, namely, the mass transfer limitation of the traditional intermittent reaction is overcome through the synergism of the laminar shear effect and the ultrasonic cavitation effect of a continuous flow reaction device, so that the coupling reaction time of the palmitoyl tripeptide-38 is shortened, the occurrence rate of side reaction is reduced, and the universality verifies that the solvent system has excellent performance in synthesis of the palmitoyl tripeptide-38, and the solvent system is suitable for industrial production. And the coupling efficiency is stable.

The invention discloses application of a caenorhabditis elegans reproductive disorder model to screening of strains promoting reproduction health, and relates to the technical field of biologica.Cyclophosphamide medicine intervention is applied to caenorhabditis elegans, so that the normal reproductive system of caenorhabditis elegans is damaged, and the reproductive disorder model is prepared; then 3985 gene knockout strains in an escherichia coli Keio gene knockout library are utilized, and strains with the reproductive capacity remarkably improved for reproductive disorder are screened; and carrying out homologous gene knockout on the probiotics by using a gene editing technology, and screening out the probiotics with the potential of promoting reproductive health. The reproductive disorder model is stable and repeatable, can be used for screening strains or drugs for improving the reproductive function, and also can be used for screening candidate strains or drugs for reducing the reproductive toxicity of cyclophosphamide. According to the method, an effective tool is provided for screening of reproduction-related probiotic strains or screening of related drugs, and meanwhile, the strains screened based on the model provide new candidate strains for treatment and research of reproductive disorder diseases.

The invention discloses an application of triptophenol terpene in preparation of a medicine for treating diabetic nephropathy, belongs to the technical field of medicines, is obtained by separating and purifying tripterygium glycosides extract, and is applied as a single active component or a core component in DKD treatment medicines, and solves the problems that a traditional tripterygium glycosides preparation is high in toxicity and uncontrollable in curative effect. The medicine of the triptophenol terpene can obviously inhibit kidney inflammatory factors (IL-6 and TNF-alpha) and fibrosis markers (TGF-beta); the urine protein level is reduced, and the kidney function is improved; and hepatotoxicity and reproductive toxicity risks are avoided.

The invention discloses an application of butyric acid or a derivative thereof in preparation of a zearalenone reproductive toxicity resisting preparation. Also disclosed is an antitoxic feed composition comprising a specific base ration and a butyric acid derivative. In-vivo and in-vitro tests and molecular mechanism researches prove that butyric acid and derivatives thereof (such as tributyrin and sodium butyrate) can effectively relieve zearalenone (ZEN)-induced animal ovary injury. The action mechanism of the traditional Chinese medicine composition is that follicular atresia is reduced, the balance of reproductive hormones (such as GnRH, E2, AMH and P4) is recovered, and clinical symptoms caused by ZEN poisoning such as vulva swelling and the like are relieved by activating a BMPs / SMAD signal channel, especially up-regulating SMAD4 protein expression and inhibiting oxidative stress (ROS) and Caspase-3 mediated granular cell apoptosis.

The invention relates to application of Chinese yam protein, in particular to application of Chinese yam active protein in preparation of medicine for regulating blood testis barrier to treat testis dysfunction. L-YP has unique fluorescence characteristics and remarkable antioxidant activity, and can effectively improve testis immune microenvironment disorder by repairing blood testis barriers and inhibiting NLRP3 and TLR4 / MyD88 / NF-kappa B inflammatory pathways. In combination with a molecular docking technology, a molecular mechanism that key components such as YP2 (mitochondrial ornithine aminotransferase) and the like realize immunoregulation through high-affinity binding is clarified for the first time. According to the application disclosed by the invention, a molecular docking technology is creatively combined with system experimental analysis, and a molecular mechanism of improving testis injury by the Chinese yam protein is illuminated from the perspective of a'structure-function-target 'synergistic effect, so that a novel dietary intervention strategy is provided for preventing reproductive toxicity caused by chemotherapy; the application potential of medicinal and edible components in the field of male reproductive health is highlighted.

The invention relates to the technical field of environmental medicine and reproductive toxicology, in particular to application of aspirin in prevention and / or treatment of male reproductive injury caused by rare earth elements. The invention also relates to a method for improving sperm quality reduction caused by rare earth element exposure. The method comprises the step of administering an effective dose of aspirin or a pharmaceutically acceptable salt thereof to an individual in need thereof. The invention also relates to a pharmaceutical composition for preventing and / or treating male reproductive injury caused by rare earth elements. The cognitive limitation of a traditional oxidative stress mechanism is broken through, a new mechanism of rare earth reproductive toxicity is disclosed from a new perspective of testis immune microenvironment for the first time, and an intervention scheme aiming at the new mechanism is provided. According to the invention, the aspirin is creatively used for solving the novel public health problem of low-dose long-term exposure of rare earth elements for the first time, and a new application field is developed for non-steroidal anti-inflammatory drugs.

The invention relates to the technical field of medicines, and discloses an application of lupin or an extract thereof in preparation of a drug for relieving reproductive toxicity of chemotherapeutic drugs, the lupin is selected from lupin plants, preferably lupin variety Jenabillup, and the chemotherapeutic drugs comprise busulfan, cyclophosphamide or cis-platinum. The administration dosage of the lupin or the extract thereof is 0.1-100 mg / kg body weight, the administration period is continuous administration for at least 5 weeks, experiments show that the lupin can significantly improve the sperm concentration and sperm motility of chemotherapy model animals and improve pathological damage of testicular tissues, the mechanism relates to antioxidant, anti-inflammatory and anti-apoptosis effects, and the lupin or the extract thereof can be used for preparing a chemotherapy model animal. The invention provides a safe and effective new strategy for fertility protection of chemotherapy patients.

The invention discloses an application of a tetrahydroindazole derivative in preparation of drugs for resisting reproduction toxicity of bisphenol compounds, the tetrahydroindazole derivative is specifically N-(4-(3-fluorophenoxy) phenyl)-N '-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide, the molecular formula is C16H17F3N2O3, and the structural formula is shown in the formula (I). By investigating the influence of N-(4-(3-fluorophenoxy) phenyl)-N '-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide on the egg laying amount of caenorhabditis elegans, the obtained experimental result shows that N-(4-(3-fluorophenoxy) phenyl)-N'-(4, 5, 6, 7-tetrahydro-1H-indazole-5-) oxamide has the effect of resisting reproductive toxicity of bisphenol compounds, and can be used for preparing the caenorhabditis elegans egg laying agent. The total egg laying amount of the nematodes after bisphenol S exposure can be remarkably increased to be close to the level of a control group, so that the method is expected to be applied to preparation of various bisphenol compound reproductive toxicity resisting drugs.

The invention relates to the technical field of environmental medicine and reproductive toxicology, in particular to application of aspirin in prevention and / or treatment of male reproductive injury caused by rare earth elements. The invention also relates to a method for improving sperm quality reduction caused by rare earth element exposure. The method comprises the step of administering an effective dose of aspirin or a pharmaceutically acceptable salt thereof to an individual in need thereof. The invention also relates to a pharmaceutical composition for preventing and / or treating male reproductive injury caused by rare earth elements. The cognitive limitation of a traditional oxidative stress mechanism is broken through, a new mechanism of rare earth reproductive toxicity is disclosed from a new perspective of testis immune microenvironment for the first time, and an intervention scheme aiming at the new mechanism is provided. According to the invention, the aspirin is creatively used for solving the novel public health problem of low-dose long-term exposure of rare earth elements for the first time, and a new application field is developed for non-steroidal anti-inflammatory drugs.

The application belongs to the technical field of reproductive health, and particularly relates to a new application of a testicular metabolite 2-n-propylthiazolidine-4-carboxylic acid (PTCA), and the 2-n-propylthiazolidine-4-carboxylic acid is used for preparing a drug for treating testicular spermatogenic disorder induced by AFB1. The PTCA can promote the secretion of testosterone of testicular interstitial cells, activate the PI3K / AKT / NRF2 antioxidant signaling pathway in testicular tissue, relieve testicular injury induced by AFB1, and reduce the ROS level in testicular tissue. The application discloses, for the first time, the important role of the PTCA as a new testicular metabolic marker and protective agent in the male reproductive system, provides a new insight into the reproductive toxicity mechanism of AFB1, and lays an experimental foundation for the application of metabolites to the intervention strategy of male spermatogenic disorder.

The application discloses a biomarker for evaluating bisphenol S male reproductive toxicity and application thereof, and belongs to the technical field of biological medicine. The biomarker for evaluating bisphenol S male reproductive toxicity is screened out based on multi-omics integration and functional phenotype, and the biomarker comprises one or more of a Cyp2e1 gene, a Tdo2 gene and a Ugt1a1 gene. The expression amount of the above biomarker is detected by a detection reagent to evaluate the male reproductive toxicity of bisphenol S, and the detection reagent has high accuracy and specificity. A corresponding detection kit and a use method are developed, and the male reproductive toxicity of bisphenol S can be quickly and accurately evaluated.

The present application relates to the technical field of breeding, in particular to an indoor breeding of the formula feed of the chemi-smithia brycei and the breeding method of the chemi-smithia brycei, the formula feed is added with the compound essential oil of eugenol, citral and cinnamic acid (mass ratio 0.8:0.5:0.3), the formula feed is low in mouldy rate and zero in formaldehyde residue. The breeding method comprises the following steps: directly feeding the fermented feed to the newly hatched larvae (without the transition of rice stems), feeding the low-density 3rd instar larvae in different pipes (10 larvae per dish), and replacing the feed only once in the whole process. The application of the present application completely eliminates the reproductive toxicity of chemical preservatives and is suitable for large-scale year-round breeding of standard insect sources.

The application discloses a method for evaluating the reproductive toxicity of microplastics, comprising the following steps: (1) providing a FXR1 protein phase separation report system, which is selected from an in vitro phase separation system or an intracellular report system; (2) contacting a microplastic sample to be tested with the FXR1 protein phase separation report system; (3) observing and detecting the influence of the microplastic sample to be tested on the FXR1 protein phase separation phenomenon in the report system by using fluorescence microscopic imaging technology, wherein the influence includes the morphological change of FXR1 protein phase separation droplets and / or the flowability change of FXR1 protein phase separation droplets; (4) evaluating the reproductive toxicity risk level of the microplastic sample to be tested based on the detection result of step (3).

This application provides a novel tabletop ventilation device, relating to the field of reproductive toxicity testing technology for non-clinical safety evaluation. It includes a hood and an exhaust duct. The hood is generally polyhedral, with an openable and closable door on at least one side. One end of the exhaust duct is connected to at least one hood, and the other end is connected to an exhaust system for discharging air from inside the hood to the outside of the laboratory. The hood is placed on a tabletop within the laboratory. The internal space of the hood is used to accommodate a microscope and specimens. The hood has an observation window, and the eyepiece of the microscope can be aligned with the observation window. By covering the microscope with the hood, the internal space of the hood is relatively sealed, which can confine the formaldehyde gas volatilized from formalin to a controllable area. When specimens are placed inside the hood for operation, the external exhaust system can provide power to promptly expel the air containing formaldehyde and other pollutants from the hood, effectively reducing the occupational exposure risks of respiratory damage and skin allergies for laboratory personnel.

The invention relates to the technical field of plant and edible microorganism product separation, and relates to a toadstool phenolic acid glycoside extract and a preparation method and application thereof.The extraction method of the toadstool glycoside extract comprises the following steps that S1, toadstool is dried, smashed, sieved and degreased, and toadstool powder is obtained; s2, adding a solvent into the morchella esculenta powder, and performing ultrasonic treatment, filtration and concentration to obtain a crude extract; s3, deproteinizing the crude extract, passing through macroporous resin, and freeze-drying to obtain the toadstool glycoside extract, the ultrasonic power is 200-400 W, the ultrasonic temperature is 40-56 DEG C, and the ultrasonic time is 20-46 min. According to the method, the extraction rate of toadstool glycoside is remarkably increased by optimizing parameters. The toadstool glycoside extract disclosed by the invention can be used for increasing the nematode hatching rate, the total egg laying number and the brood number, inhibiting germ cell apoptosis and remarkably relieving anti-reproductive toxicity.

The invention relates to tetrapeptide-30 and a preparation method and application thereof.According to the tetrapeptide-30, low-toxicity and biodegradable NFM and anisole are compounded according to the volume ratio of 1: 1, and the tetrapeptide-30 has strong polarity and moderate hydrophobicity and can completely replace DMF. According to the mixed solvent, the solubility of Fmoc amino acid monomers, activating reagents and resin is improved, the risk of reproductive toxicity is avoided, and the volatility is reduced compared with that of DMF (Dimethyl Formamide); the method has the advantages that the process is synergistically strengthened, the mass transfer limitation of the traditional batch-type reaction is overcome through the synergism of the laminar shear effect and the ultrasonic cavitation effect of a continuous flow reaction device, the coupling reaction time of the tetrapeptide-30 is shortened, the side reaction occurrence rate is reduced, and the universality verifies that the solvent system has excellent performance and stable coupling efficiency in the synthesis of the tetrapeptide-30.

The invention relates to myristoyl pentapeptide-17 and a preparation method and application thereof.According to the myristoyl pentapeptide-17, low-toxicity and biodegradable NFM and anisole are compounded according to the volume ratio of 1: 1, and the myristoyl pentapeptide-17 has strong polarity and moderate hydrophobicity and can completely replace DMF. According to the mixed solvent, the solubility of Fmoc amino acid monomers, activating reagents and resin is improved, the risk of reproductive toxicity is avoided, and the volatility is reduced compared with that of DMF (Dimethyl Formamide); the process is synergistically enhanced, namely, the mass transfer limitation of the traditional batch-type reaction is overcome through the synergism of the laminar shear effect and the ultrasonic cavitation effect of a continuous flow reaction device, so that the coupling reaction time of the myristoyl pentapeptide-17 is shortened, the occurrence rate of side reaction is reduced, and the universality verifies that the solvent system has excellent performance in the synthesis of the myristoyl pentapeptide-17, and has a wide application prospect in the synthesis of the myristoyl pentapeptide-17. And the coupling efficiency is stable.

This invention relates to the fields of environmental toxicology and organoid technology, and particularly to a method for assessing the toxicity of environmental microplastic exposure based on testicular organoids. The method includes: processing waste testicular tissue into a single-cell suspension, the single-cell suspension comprising supporting cells, germ cells, and interstitial cells; seeding the single-cell suspension onto a biomimetic hydrogel scaffold for culture to obtain mature testicular organoids, which are then cultured in a target microplastic exposure solution to establish a microplastic exposure model; determining the raw values ​​of toxicity assessment indicators corresponding to the microplastic exposure model, including: morphological score data, grayscale values ​​of cell marker expression, DNA damage indicators, testosterone secretion levels, and cytotoxicity indicators; calculating a comprehensive toxicity score based on the raw values ​​of each toxicity assessment indicator, and assessing the reproductive toxicity risk level of the target microplastic exposure based on the comprehensive toxicity score. This application can improve the accuracy of assessing the reproductive toxicity risk level of microplastic exposure.

The invention discloses an application of targeted JNK1 / MAPK in treatment of asthenospermia, and relates to the technical field of biological materials, and the technical key points are as follows: a DBP-exposed filial generation SD rat model is constructed; the specific molecular mechanism that DBP regulates and controls a JNK1 / MAPK signal channel to promote apoptosis of testicular germ cells and induce reproductive toxicity is comprehensively verified by combining network pharmacology, transcriptome sequencing technology analysis, related molecular biology experiments and the like; by preparing the medicine for reducing the JNK1 / MAPK protein level, the medicine can be used for treating asthenospermia caused by DBP exposure, and a new thought is provided for treatment and prevention of DBP-induced reproductive toxicity.

Nicotinamide mononucleotide derivatives of Formula (I)for use in the treatment and / or prevention of an antineoplastic-induced toxicity. Also, pharmaceutical compositions that include compounds of Formula (I) for use in the treatment and / or prevention of an antineoplastic-induced toxicity selected from cardiotoxicity, nephrotoxicity, haematotoxicity, hepatotoxicity, lymphoid toxicity, gastrointestinal toxicity, dermal toxicity, reproductive toxicity, bone toxicity, genetic toxicity and bladder toxicity.

The present invention relates to the field of biopharmaceutical technology, and in particular, to use of a nitroimidazole derivative or a pharmaceutical combination in the preparation of a medicament against drug-sensitive tuberculosis. Animal studies, phase I and phase II clinical trials, and the optimization of dosage and route of administration have demonstrated that the nitroimidazole derivative has no significant drug accumulation, no significant reproductive toxicity, no significant cardiotoxicity, and no QT prolongation effects. Compared with existing drugs such as delamanid, the nitroimidazole derivative has shorter treatment time, more significant therapeutic effects, and higher safety. Especially in pulmonary images, the cavities in patients are significantly reduced, or the lesions are significantly absorbed, suggesting that the nitroimidazole derivative is promising as a novel drug for preventing and treating drug-sensitive tuberculosis.

The application discloses a method for constructing a zebrafish reproductive toxicity model, a method for evaluating reproductive toxicity of a chemical substance and application, and relates to the technical field of reproductive development toxicity of a chemical substance. The method for evaluating reproductive toxicity of a chemical substance provided by the application can focus on changes in courtship behavior by taking behavior pictures or videos, does not need to sacrifice test animals, and does not need professional fluorescence imaging instruments and equipment and tissue section equipment; the method is more in line with animal ethics, saves cost, and can realize continuous observation by using the same batch of organisms. Test courtship behavior can be quickly analyzed by using software to obtain changes in zebrafish behavior. The evaluation method provided by the application can detect ovarian malformation of females and male reproductive toxicity, and has the advantage of more evaluation dimensions. Based on the method provided by the application, a zebrafish reproductive toxicity model can be constructed, and the model can be used as a drug screening platform for damage of reproductive function of zebrafish.

The invention relates to palmitoyl pentapeptide-3 and a preparation method and application thereof.According to the palmitoyl pentapeptide-3, low-toxicity and biodegradable NFM and anisole are compounded according to the volume ratio of 1: 1, and the palmitoyl pentapeptide-3 has strong polarity and moderate hydrophobicity and can completely replace DMF. According to the mixed solvent, the solubility of Fmoc amino acid monomers, activating reagents and resin is improved, the risk of reproductive toxicity is avoided, and the volatility is reduced compared with that of DMF (Dimethyl Formamide); the process is synergistically enhanced, namely, the mass transfer limitation of the traditional batch-type reaction is overcome through the synergism of the laminar shear effect and the ultrasonic cavitation effect of a continuous flow reaction device, so that the coupling reaction time of palmitoyl pentapeptide-3 is shortened, the occurrence rate of side reaction is reduced, and the universality verifies that the solvent system has excellent performance in synthesis of palmitoyl pentapeptide-3, and has a wide application prospect in the synthesis of palmitoyl pentapeptide-3. And the coupling efficiency is stable.