149 results about "Hydrogel scaffold" patented technology

The invention discloses a biphasic hydrogel scaffold of a bionic bone cartilage matrix as well as a preparation method and application of the biphasic hydrogel scaffold, and belongs to the technical field of tissue engineering. The biphasic hydrogel scaffold of the bionic bone cartilage matrix provided by the invention comprises a hard bone matrix phase and a cartilage matrix phase. Wherein hard chitosan hydrogel with a porous structure is used as a hard bone matrix phase, and soft chitosan hydrogel is used as a cartilage matrix phase. The biphase hydrogel with the asymmetric matrix stiffness and the biochemical microenvironment can effectively release active substances in a controlled mode, the release period exceeds 21 days, the time requirement for osteochondral repair is met, and lasting support is provided for proliferation and differentiation of cells. Meanwhile, the biphase hydrogel provided by the invention has good biological safety, two matrix phases have different mechanical properties, the biphase hydrogel adapts to in-vivo complex physiological and mechanical environments, the fate of stem cells can be directionally regulated and controlled, synchronous regeneration and repair of osteochondral tissues are realized, and the biphase hydrogel has a wide application prospect.

Aiming at the challenge of osteochondral defect repair, the invention provides a preparation method of a fiber-reinforced self-repairing hydrogel scaffold (SBPS). According to the method, phenylboronic acid modified sodium alginate, polyvinyl alcohol and fibroin nanofibers rich in beta-Sheet are utilized to form the hydrogel through dynamic boric acid ester bonds and hydrogen bonds. The SBPS hydrogel loaded with PDGF-BB is combined with the nanosheet containing LDH / ChS / TGF-beta3, so that multi-dimensional synergy of mechanical strengthening, sequential drug release (PDGF-BB is released in an early stage and TGF-beta3 / Mg < 2 + > is released in a later stage), ROS removal and endogenous repair is realized. The Mg < 2 + > concentration gradient effect further assists staged regulation (vascularized bone regeneration-cartilage matrix synthesis), and breaks through the limitation of a single factor. Experiments prove that the scaffold can effectively drive angiogenesis, stem cell recruitment and cartilage / bone differentiation in vivo and in vitro, and finally osteochondral regeneration with structure-function matching is achieved.

The invention provides a piezoelectric composite stent, a preparation method thereof and application of the piezoelectric composite stent in tracheal defect repair, and belongs to the technical field of tissue engineering. The preparation method of the piezoelectric composite stent comprises the following steps: (1) immersing a PLLA stent into an FF polypeptide solution to prepare a PLLA-FF stent; (2) the PLLA-FF stent is immersed in a tannic acid solution, and a PLLA-FF-TA composite stent is prepared; (3) preparing oxygen-containing PLGA (poly (lactic-co-glycolic acid)) nano microbubbles, and mixing the GelMA with the oxygen-containing PLGA nano microbubbles to obtain a Gel-NB hydrogel precursor; (4) injecting the Gel-NB hydrogel precursor into the PLLA-FF-TA composite scaffold, and carrying out ultraviolet light cross-linking on the Gel-NB hydrogel precursor and the PLLA-FF-TA composite scaffold; and carrying out ultrasonic irradiation to obtain the PLLA-FF-TA (at) Gel-Nb + US composite scaffold. The piezoelectric hydrogel stent is successfully prepared, can be well used for tracheal defect repair, has the advantages of good degradation performance, high piezoelectric performance, long-term stability and high strength, and is beneficial to clinical transformation and application.

The invention relates to a two-phase hydrogel system based on space-time gradient regulation and control as well as a preparation method and application of the two-phase hydrogel system. The biphase hydrogel system can also be expressed as a GelMA composite hydrogel scaffold of a porous structure, the whole biphase hydrogel system is of a core-shell structure, the inner layer core mineralizes SilMA hydrogel microspheres, the porous GelMA hydrogel scaffold is used as an outer layer, and the angiogenesis promoting factor dimethyl oxalaminoacetic acid, the human umbilical vein endothelial cells and the bone marrow mesenchymal stem cells are loaded on the outer layer. Wherein the inner-layer core mineralized SilMA hydrogel microspheres realize continuous release of osteogenesis signals by slowly releasing nHAp, and the outer-layer porous GelMA hydrogel stent releases DMOG by rapid degradation to realize early angiogenesis, so that space-time coupling construction of blood vessels and bone tissues is realized. Through differential degradation characteristics of inner and outer layers of hydrogel, precise time sequence cooperative regulation and control of angiogenesis and bone tissue remodeling can be realized, and the bionic property and function integration capability of a vascularized bone organ structure can be improved, so that the clinical transformation potential of the vascularized bone organ structure is improved.

The invention discloses a diabetic bone defect repair hydrogel stent capable of slowly releasing exosome and a preparation method of the diabetic bone defect repair hydrogel stent. The preparation method comprises the following steps: preparing biotinylated quaternized chitosan methacrylate (QCS-MA-Biotin), formylated oxidized dextran and a biotinylated exosome (Exo-Bio), carrying out avidin bridging, and carrying out photocuring, so as to form the double-network hydrogel. The scaffold disclosed by the invention has a pH-responsive slow-release characteristic, the 28-day release rate of the exosome is less than or equal to 50%, and the activity retention rate gt; in addition, the repair of the diabetic bone defect can be obviously promoted. The method is suitable for repairing defects of skull, alveolar bone and the like.

The invention provides a self-expanding hydrogel intravascular stent and a preparation method thereof, and belongs to the technical field of medical instruments, the intravascular stent is of a hollow tubular structure, and the tube wall is of a sealed type; the intravascular stent is made of a hydrogel material. After the hydrogel stent shrinks, the size of the hydrogel stent is obviously reduced, the hydrogel stent can be delivered to a target position in a balloon intervention mode, and the hydrogel stent can stay at the target position and provide a supporting effect on the hydrogel stent due to the fact that the hydrogel stent has a self-expansion effect.

The invention discloses a double-layer hydrogel stent capable of releasing medicine in time sequence as well as a preparation method and application of the double-layer hydrogel stent. The scaffold comprises an inner hydrogel and an outer hydrogel coating the inner hydrogel, the outer hydrogel is loaded with cell factors, and the cell factors have the following effects of collecting neutrophils and promoting polarization of the neutrophils into subtypes with stem cell collection; the inner layer hydrogel is loaded with neurotrophic factors. According to the hydrogel scaffold, ordered slow release of cell factors (such as IL-8) and neurotrophic factors (such as NGF) is achieved through the structural design of the inner hydrogel layer and the outer hydrogel layer, neutrophils and sensory nerves can be activated in sequence in a bone defect area, the neutrophils are collected in the defect area, stem cell homing is promoted, and osteogenesis is started; then, local sensory nerves are induced to grow in through the lagged and continuously released NGF, the sensory nerves are stimulated to play an immune regulation role, inflammation dissipation is promoted, and finally repair and regeneration of refractory bone defects are promoted.

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The invention discloses a preparation method of a functional bone repair hydrogel scaffold, which comprises the following steps: mixing nano attapulgite and methacrylated gelatin to form a printable emulsion system, performing three-dimensional printing on the printable emulsion system, and performing ultraviolet crosslinking to form a porous three-dimensional scaffold, and incubating the porous three-dimensional scaffold in a PBS (Phosphate Buffer Solution) containing the mesenchymal stem cell exosome and the miRNA-26a-5p and / or the miRNA-30a-5p, so that the mesenchymal stem cell exosome, the miRNA-26a-5p and / or the miRNA-30a-5p are co-loaded on the porous three-dimensional scaffold. The invention also provides a scaffold prepared by the method and application of the scaffold in biology. The scaffold can load mesenchymal stem cell exosomes and specific micro-RNA, and realizes regulation and control of immune microenvironment of injured parts and promotion of bone regeneration, so that the repair effect of large-volume bone defects is effectively improved.

The invention provides an ultrasonic stimulation response hydrogel stent as well as a preparation method and application thereof. A coaxial 3D printing technology and surface chemical modification are adopted to prepare the ultrasonic stimulation response hydrogel stent with immunoregulation and osteogenesis promotion functions. The hydrogel with the skin-core structure comprises core layer hydrogel and skin layer hydrogel, wherein the core layer hydrogel is composed of methacrylated gelatin chelated metal ions modified by a diphosphonic acid compound and a methacrylate modified polymer, and the skin layer hydrogel is composed of methacrylated gelatin modified by an immunoregulatory factor and an organic sound-sensitive agent. The ultrasonic stimulation response hydrogel stent has the advantages of ultrasonic stimulation response release, good biocompatibility, macrophage M2 type polarization regulation, angiogenesis promotion, bone regeneration promotion and the like, and has potential application prospects in the field of bone defect regeneration and repair.

The invention discloses an infectious bone defect bionic repair hydrogel stent and a preparation method and application thereof, and is characterized in that the infectious bone defect bionic repair hydrogel stent is composed of an upper antibacterial-vascularization layer simulating a periosteum structure, a middle support layer simulating a bone cortex structure and a lower regeneration-regulation layer simulating a cancellous bone structure; the upper antibacterial-vascularization layer is prepared from photoresponse CuxO / SiC heterojunction powder, the middle support layer is prepared from silk fibroin, and the lower regeneration-regulation layer is prepared from HAP (at) Cur-CNDs-Pt NPs composite nanoparticles. The multi-dimensional structure and function integration realizes the full-period regulation and control of antibiosis-barrier-regeneration, breaks through the limitation of the traditional single-function stent, and provides an integrated solution for infectious bone defect repair.

The present invention is applicable to the technical field of bone defect repair, and provides a preparation method and application of a collateral-modified Gelma hydrogel. The present invention performs collateral modification on the Gelma hydrogel by Fibrinogen, successfully improving the mechanical properties and cell adhesion of the hydrogel scaffold. At the same time, the loading of NELL‑1 protein significantly improves the scaffold's ability to repair bone defects. The Fibrinogen‑Gelma / NELL‑1 hydrogel scaffold constructed by the present invention has shown ideal mechanical properties in relevant characterizations, and has been shown to play a key role in the repair of bone tissue defects in in vitro and in vivo experiments, and has greatly promoted the expression of osteogenesis-related genes. Therefore, the present invention is expected to be applied to clinical treatments related to bone defect repair, such as maxillary sinus bone augmentation and guided bone regeneration (GBR), in the future.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

The invention discloses a flexible actuator and a preparation method thereof. And combining the hydrogel film with the inverse opal photonic crystal structure with PDMS (Polydimethylsiloxane) to prepare the hydrogel stent actuator capable of treating cervical stenosis. The contact angle of the prepared hydrogel film with the inverse opal photonic crystal structure can reach 90 degrees or below, and the hydrogel film has strong affinity to water on a super-hydrophobic surface. Due to the super-hydrophobicity, the thin film can be tightly attached to an air-water interface while keeping self stable floating. The cervical stent disclosed by the invention has controllable adhesiveness, adjustable form, stimulation responsiveness and drug release property, the stent can be retained at the cervical part, and the application potential of the cervical stent in the aspect of treating cervical stenosis is shown.

The invention relates to a preparation method of an exosome-loaded photocuring hydrogel scaffold capable of promoting bone repair and a product thereof, and relates to the technical field of biological materials and tissue engineering. According to the main thought, exosome is loaded in gelatin hydroxyapatite microspheres, then the microspheres are evenly dispersed in a three-dimensional continuous network formed by light-cured hydrogel, and a microsphere matrix and a light-cured hydrogel material are the same or different. The invention has the advantages that: the photocuring hydrogel provides macroscopic physical support and a cell adhesion skeleton; and the dispersed exosome loaded hydroxyapatite microspheres are used as an independent repository, so that protection and controllable release of active substances are realized, and meanwhile, the mechanical strength of the hydrogel is enhanced. And the structure is accurate and controllable, the composite slurry can be molded into any complex two-dimensional or three-dimensional structures through a mold or a biological 3D printing technology by utilizing the photocuring characteristic, and the personalized repair requirement is met.

The invention belongs to the technical field of biomedical material engineering, and discloses a hydrogel scaffold used for 3D tumor sphere culture and drug screening, the scaffold is formed by four-arm polyethylene glycol maleimide, fibrinogen and thrombin through Michael addition crosslinking and biopolymerization synergistically, the elastic modulus of 10-1000 Pa can be adjusted by regulating and controlling the proportion of all the components, and the hydrogel scaffold can be used for 3D tumor sphere culture and drug screening. Gel forming is completed within 5 min at the room temperature. The scaffold has the biological activity of fibrous protein and the structural stability of polyethylene glycol, can efficiently support HCT116 cells to form high-activity tumor balls, accurately simulate the in-vivo tumor microenvironment and remarkably improve the accuracy and repeatability of drug screening, is easy and convenient to operate, is compatible with a standard pore plate high-throughput platform and is suitable for drug sensitivity detection of personalized treatment of tumors.

The invention belongs to the technical field of artificial meat tissue engineering, and particularly relates to a spiral composite hydrogel scaffold as well as a preparation method and application thereof. The spiral composite hydrogel scaffold provided by the invention adopts a bionic microstructure design, and can simulate fiber arrangement and a multi-scale hierarchical structure of natural beef tissues, so that ordered distribution of muscle cells and fat cells is guided in a microscopic space. By optimizing hydrogel components and a cross-linking mode, the mechanical strength and the elasticity of the stent are controllable in a relatively large range. A three-dimensional culture environment constructed by using the scaffold can promote differentiation and synchronous maturation of beef related muscle cells and fat cells, the time of a texturization process is greatly shortened, and the fibrosis degree and marbling effect of artificial meat are improved. Compared with a traditional two-dimensional culture method or a single scaffold method, the method can efficiently obtain textured artificial meat with a bionic structure and a texture close to that of natural beef, and is suitable for the fields of artificial meat manufacturing, functional food development, tissue engineering and the like.

The invention discloses a melanin-containing multi-layer structural color hydrogel microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that a colloidal particle solution is injected into a microneedle mold, and a colloidal crystal microneedle template is obtained through a deposition method; preparing a hydrogel pre-polymerization solution from melanin and a high-molecular organic matter pre-polymerization solution, injecting the hydrogel pre-polymerization solution into a microneedle mold containing a colloidal crystal microneedle template, and curing the hydrogel pre-polymerization solution to obtain a colloidal crystal hydrogel mixed microneedle patch; after the colloidal crystal microneedle template is removed, the inverse opal structure hydrogel scaffold containing melanin is obtained; preparing a pre-gel solution of a drug-added temperature-sensitive polymer material, pouring the pre-gel solution into holes of the prepared inverse opal structure hydrogel stent, and cooling and curing to form a shell layer, thereby obtaining the hydrogel stent. The hydrogel microneedle patch provided by the invention contains an inverse opal structure bracket with photo-thermal conversion capacity and inherent immunoregulation performance and a temperature-sensitive gel outer layer loaded with drug components, and integrates multiple functions of photo-thermal response, drug release, immunoregulation, sensing and the like.

The application provides a DNA tetrahedron-based complex and a preparation method and application thereof, and belongs to the field of medicines. The application designs a functionalized DNA tetrahedron complex modified bioactive composite hydrogel scaffold containing BMP-2, combines the functionalized BMP-2 binding peptide with the tetrahedron framework structure with unique three-dimensional spatial structure and fine programmability through a chemical clicking mode, and further prepares the composite hydrogel scaffold by combining with methacrylated hyaluronic acid. The functionalized DNA tetrahedron complex realizes the performance of efficiently capturing BMP-2, and the composite hydrogel scaffold realizes the targeted capture of endogenous BMP-2 in the early stage of bone defect and stimulates the osteogenic differentiation of stem cells to promote in-situ osteogenesis, avoids a series of complications caused by the delivery of exogenous bone morphogenetic protein-2, and solves the problems of poor in-vivo stability, safety and high cost, and has a good application prospect.

The application discloses a 3D-printed hydrogel support and a preparation method thereof, relates to the technical field of hydrogel materials, and aims to improve the antibacterial property and biocompatibility of the hydrogel material by modifying the raw material. The application first uses gelatin as the raw material, and processes the gelatin to increase the content of surface amino groups, so that the subsequent reaction is facilitated; then, the application also uses 2,5-thiophene dicarboxylic acid as the raw material, and controls the reaction conditions to react the 2,5-thiophene dicarboxylic acid with 2-aminobenzimidazole, so as to generate an antibacterial intermediate containing a free carboxyl group, a thiophene ring and an imidazole ring structure; the antibacterial intermediate is combined in the gelatin chain segment in the form of an amide bond, then under the action of a photoinitiator, the amide bond is broken to generate an acyl radical, and the acyl radical is further coupled and grafted with an acrylic ester double bond, so as to form a hydrogel cross-linking structure, and the hydrogel material is provided with sufficient compressive strength.

The present application relates to the technical field of biomedical materials, and particularly relates to a 3D-printed self-produced electricity hydrogel scaffold for promoting wound healing and a preparation method and application thereof, comprising an anode scaffold and a cathode scaffold, the anode scaffold is prepared from a zinc-containing hydrogel, and the cathode scaffold is prepared from an iron-containing hydrogel; the anode scaffold further comprises a prepolymerization solution prepared from dopamine, ammonium persulfate, N,N'-methylene bisacrylamide, acrylamide and tetramethylethylenediamine; the hydrogel scaffold can gradually degrade to release metal ions, play an anti-inflammatory and angiogenic role, can remove free radicals such as active oxygen and active nitrogen, and play an antioxidant role; the output voltage can be stabilized for more than 7 days, the expression of repair-related genes can be improved through physiological micro-current stimulation, the proliferation and migration of fibroblasts can be promoted, the wound can be accelerated to realize scarless healing within 16 days, and the hydrogel scaffold has a wide application prospect.

The application discloses a hydrogel support for urethral wound repair and healing, which comprises an antifouling surface layer, a ZEIN middle layer and a PNIPAM hydrogel bottom layer, wherein the antifouling surface layer is a cross-linked layer added with an antibacterial agent, and the PNIPAM hydrogel bottom layer is loaded with a repair drug. The application utilizes the characteristics that the temperature of urine is higher than that of the urethra, utilizes the temperature-sensitive characteristics of poly (N-isopropyl acrylamide), enables the drug loaded in the hydrogel to be released when the urine passes, is used for offsetting the negative effects caused by the tension change, and utilizes the synergistic effect of the three layers to accelerate the repair and healing of the urethral wound.

This invention belongs to the field of skin-like tissue preparation technology, and relates to a method for constructing ordered skin-like tissue with magnetic field assistance. The method involves directional cryo-casting to prepare a layered ordered hydrogel, providing skin-like seed cells, and culturing and expanding these cells in large quantities using a complete culture medium to obtain skin-like cells. The cultured skin-like cells are then co-cultured with iron oxide nanoparticles to prepare magnetized skin-like cells. These magnetized skin-like cells are then co-cultured with the layered ordered hydrogel, and magnetic fields are applied to both sides to induce the magnetized skin-like cells to enter the layered ordered hydrogel, resulting in a hydrogel scaffold. After washing the obtained hydrogel scaffold, it is transferred to a complete culture medium, and keratinocytes are seeded on the surface of the hydrogel scaffold and cultured to prepare an ordered skin-like tissue. This invention can effectively regulate the migration and arrangement of fibroblasts, providing a physical basis for the arrangement of the extracellular matrix, especially collagen.

The invention relates to the technical field of preparation of composite scaffolds, in particular to a preparation method of a novel photosensitive injectable CMCSMA composite bioactive hydrogel scaffold loaded with urine-derived stem cell subpopulation exosomes, and the preparation method comprises the following steps: S1, preparing reagents and instruments; s2, extracting and culturing urine-derived stem cells; s3, carrying out flow cytometry identification and subgroup sorting on the urine-derived stem cells, wherein the subgroup sorting is based on CD133 and CD271 double-positive cell surface markers; s4, extracting and storing the exosome; s5, synthesizing methacrylated carboxymethyl chitosan; and S6, mixing the extracted exosome with a methacrylated carboxymethyl chitosan solution, so as to prepare the CMCSMA-sExos composite bioactive hydrogel scaffold. According to the invention, the exosome is loaded by the methacrylated carboxymethyl chitosan hydrogel, so that slow release and targeted delivery of the exosome are realized, and the in-vivo action time of the exosome is prolonged.

The invention relates to a living plant cell-animal cell composite scaffold as well as a preparation method and application thereof. The living plant cell-animal cell composite scaffold comprises a 3D printing hydrogel scaffold used as a muscle repair carrier and a 3D printing biological ceramic scaffold used as a bone repair carrier, wherein the 3D printing hydrogel scaffold and the 3D printing biological ceramic scaffold are assembled by simulating a muscle-skeleton structure; the hydrogel scaffold comprises a polymer matrix component, and functional plant cell components, namely diatom cells and mammalian cell components, namely muscle stem cells, which are distributed in the polymer matrix component, the biological ceramic scaffold comprises a biological ceramic scaffold matrix component and mammalian cell component bone marrow mesenchymal stem cells inoculated and loaded on the biological ceramic scaffold matrix component.

This invention discloses a method for preparing a 3D-printed hydrogel scaffold for cartilage repair, comprising: providing methacrylamide chitosan; providing 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres; obtaining a 3D-printed bio-ink for cartilage repair using methacrylamide chitosan, 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres, recombinant collagen CF-1552, and lithium magnesium silicate as main raw materials; and obtaining a 3D-printed bio-ink for cartilage repair using the 3D-printed bio-ink for cartilage repair as a raw material to obtain a 3D-printed hydrogel scaffold for cartilage repair. This 3D-printed hydrogel scaffold has advantages such as antibacterial properties and the ability to promote the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into chondrocytes, and can be used for cartilage repair, potentially bringing new curative opportunities for cartilage injury diseases.

The preparation method of the HLC-CTGF fusion protein disclosed by the invention comprises the following steps: designing a target gene synthesis and enzyme cutting site, reactivating a strain, and then sequentially carrying out expression vector construction of the fusion protein HLC-CTGF, expression of the fusion protein HLC-CTGF, purification of the fusion protein HLC-CTGF and immunoblotting of the fusion protein HLC-CTGF. The rhCol-CTGF fusion protein prepared by the preparation method disclosed by the invention is non-toxic and has good cell compatibility. The collagen can be cross-linked to prepare a hydrogel scaffold, and the collagen creates a good environment for the growth of osteoblasts by providing necessary nutrition and structural support; the CTGF is slowly released by controlling the degradation of the hydrogel, so that the defect that the expression of the CTGF in vivo has time dependence is overcome, and bone tissue repair is accelerated.

The application discloses a kind of collagen Pickering emulsion 3D printing-based cartilage regeneration scaffold and preparation method thereof.Collagen is extracted from fresh collagen extraction raw material, and purified collagen sponge is obtained, the collagen sponge is dissolved in acetic acid, and high-concentration collagen solution is obtained, then oil phase is added to the collagen solution, and high-speed emulsification is carried out using high-speed homogenizer, to obtain collagen-based Pickering emulsion, finally, computer digital modeling and low-temperature hydrogel forming equipment are used, and collagen-based Pickering emulsion is used as biomaterial ink to 3D print cartilage regeneration hydrogel scaffold, the printed scaffold is quickly frozen in liquid nitrogen, and vacuum drying is carried out, to obtain cartilage regeneration scaffold.The application develops collagen Pickering emulsion biomaterial ink with excellent printability, and through 3D printing, collagen-based cartilage regeneration scaffold with good quick customization forming property, adjustable pore structure and good biological activity is obtained.

Embodiments relate to porous hydrogel microparticles, porous granular hydrogel scaffolds, and methods of making and using thereof. A method of making porous hydrogel microparticles includes crosslinking first polymers and second polymers to form composite microgels, adding the composite microgels to a liquid solution at a first temperature to form a composite microgel suspension, reducing the temperature of the composite microgel suspension to a second temperature below a phase separation temperature such that the second polymers separate from the first polymers, and filtering the composite microgel suspension from the liquid solution such that the second polymers diffuse out of the composite microgel suspension, resulting in the porous microgels.

The application discloses a customizable biphasic hydrogel scaffold, which comprises a transparent cartilage layer and a subchondral bone layer, the transparent cartilage layer comprises methacrylated type I recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated type I recombinant collagen and resveratrol-loaded bioactive glass. The application further provides a preparation method and application of the biphasic hydrogel scaffold. The biphasic hydrogel scaffold has the advantages of customization, antioxidation, anti-inflammation, high biocompatibility, promotion of wound healing and bone repair and the like.