112 results about "Hydrogel scaffold" patented technology

The invention discloses a diabetic bone defect repair hydrogel stent capable of slowly releasing exosome and a preparation method of the diabetic bone defect repair hydrogel stent. The preparation method comprises the following steps: preparing biotinylated quaternized chitosan methacrylate (QCS-MA-Biotin), formylated oxidized dextran and a biotinylated exosome (Exo-Bio), carrying out avidin bridging, and carrying out photocuring, so as to form the double-network hydrogel. The scaffold disclosed by the invention has a pH-responsive slow-release characteristic, the 28-day release rate of the exosome is less than or equal to 50%, and the activity retention rate gt; in addition, the repair of the diabetic bone defect can be obviously promoted. The method is suitable for repairing defects of skull, alveolar bone and the like.

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The invention discloses an infectious bone defect bionic repair hydrogel stent and a preparation method and application thereof, and is characterized in that the infectious bone defect bionic repair hydrogel stent is composed of an upper antibacterial-vascularization layer simulating a periosteum structure, a middle support layer simulating a bone cortex structure and a lower regeneration-regulation layer simulating a cancellous bone structure; the upper antibacterial-vascularization layer is prepared from photoresponse CuxO / SiC heterojunction powder, the middle support layer is prepared from silk fibroin, and the lower regeneration-regulation layer is prepared from HAP (at) Cur-CNDs-Pt NPs composite nanoparticles. The multi-dimensional structure and function integration realizes the full-period regulation and control of antibiosis-barrier-regeneration, breaks through the limitation of the traditional single-function stent, and provides an integrated solution for infectious bone defect repair.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

The invention discloses a flexible actuator and a preparation method thereof. And combining the hydrogel film with the inverse opal photonic crystal structure with PDMS (Polydimethylsiloxane) to prepare the hydrogel stent actuator capable of treating cervical stenosis. The contact angle of the prepared hydrogel film with the inverse opal photonic crystal structure can reach 90 degrees or below, and the hydrogel film has strong affinity to water on a super-hydrophobic surface. Due to the super-hydrophobicity, the thin film can be tightly attached to an air-water interface while keeping self stable floating. The cervical stent disclosed by the invention has controllable adhesiveness, adjustable form, stimulation responsiveness and drug release property, the stent can be retained at the cervical part, and the application potential of the cervical stent in the aspect of treating cervical stenosis is shown.

The invention relates to a preparation method of an exosome-loaded photocuring hydrogel scaffold capable of promoting bone repair and a product thereof, and relates to the technical field of biological materials and tissue engineering. According to the main thought, exosome is loaded in gelatin hydroxyapatite microspheres, then the microspheres are evenly dispersed in a three-dimensional continuous network formed by light-cured hydrogel, and a microsphere matrix and a light-cured hydrogel material are the same or different. The invention has the advantages that: the photocuring hydrogel provides macroscopic physical support and a cell adhesion skeleton; and the dispersed exosome loaded hydroxyapatite microspheres are used as an independent repository, so that protection and controllable release of active substances are realized, and meanwhile, the mechanical strength of the hydrogel is enhanced. And the structure is accurate and controllable, the composite slurry can be molded into any complex two-dimensional or three-dimensional structures through a mold or a biological 3D printing technology by utilizing the photocuring characteristic, and the personalized repair requirement is met.

The invention belongs to the technical field of biomedical material engineering, and discloses a hydrogel scaffold used for 3D tumor sphere culture and drug screening, the scaffold is formed by four-arm polyethylene glycol maleimide, fibrinogen and thrombin through Michael addition crosslinking and biopolymerization synergistically, the elastic modulus of 10-1000 Pa can be adjusted by regulating and controlling the proportion of all the components, and the hydrogel scaffold can be used for 3D tumor sphere culture and drug screening. Gel forming is completed within 5 min at the room temperature. The scaffold has the biological activity of fibrous protein and the structural stability of polyethylene glycol, can efficiently support HCT116 cells to form high-activity tumor balls, accurately simulate the in-vivo tumor microenvironment and remarkably improve the accuracy and repeatability of drug screening, is easy and convenient to operate, is compatible with a standard pore plate high-throughput platform and is suitable for drug sensitivity detection of personalized treatment of tumors.

The invention belongs to the technical field of artificial meat tissue engineering, and particularly relates to a spiral composite hydrogel scaffold as well as a preparation method and application thereof. The spiral composite hydrogel scaffold provided by the invention adopts a bionic microstructure design, and can simulate fiber arrangement and a multi-scale hierarchical structure of natural beef tissues, so that ordered distribution of muscle cells and fat cells is guided in a microscopic space. By optimizing hydrogel components and a cross-linking mode, the mechanical strength and the elasticity of the stent are controllable in a relatively large range. A three-dimensional culture environment constructed by using the scaffold can promote differentiation and synchronous maturation of beef related muscle cells and fat cells, the time of a texturization process is greatly shortened, and the fibrosis degree and marbling effect of artificial meat are improved. Compared with a traditional two-dimensional culture method or a single scaffold method, the method can efficiently obtain textured artificial meat with a bionic structure and a texture close to that of natural beef, and is suitable for the fields of artificial meat manufacturing, functional food development, tissue engineering and the like.

The application provides a DNA tetrahedron-based complex and a preparation method and application thereof, and belongs to the field of medicines. The application designs a functionalized DNA tetrahedron complex modified bioactive composite hydrogel scaffold containing BMP-2, combines the functionalized BMP-2 binding peptide with the tetrahedron framework structure with unique three-dimensional spatial structure and fine programmability through a chemical clicking mode, and further prepares the composite hydrogel scaffold by combining with methacrylated hyaluronic acid. The functionalized DNA tetrahedron complex realizes the performance of efficiently capturing BMP-2, and the composite hydrogel scaffold realizes the targeted capture of endogenous BMP-2 in the early stage of bone defect and stimulates the osteogenic differentiation of stem cells to promote in-situ osteogenesis, avoids a series of complications caused by the delivery of exogenous bone morphogenetic protein-2, and solves the problems of poor in-vivo stability, safety and high cost, and has a good application prospect.

The application discloses a 3D-printed hydrogel support and a preparation method thereof, relates to the technical field of hydrogel materials, and aims to improve the antibacterial property and biocompatibility of the hydrogel material by modifying the raw material. The application first uses gelatin as the raw material, and processes the gelatin to increase the content of surface amino groups, so that the subsequent reaction is facilitated; then, the application also uses 2,5-thiophene dicarboxylic acid as the raw material, and controls the reaction conditions to react the 2,5-thiophene dicarboxylic acid with 2-aminobenzimidazole, so as to generate an antibacterial intermediate containing a free carboxyl group, a thiophene ring and an imidazole ring structure; the antibacterial intermediate is combined in the gelatin chain segment in the form of an amide bond, then under the action of a photoinitiator, the amide bond is broken to generate an acyl radical, and the acyl radical is further coupled and grafted with an acrylic ester double bond, so as to form a hydrogel cross-linking structure, and the hydrogel material is provided with sufficient compressive strength.

The present application relates to the technical field of biomedical materials, and particularly relates to a 3D-printed self-produced electricity hydrogel scaffold for promoting wound healing and a preparation method and application thereof, comprising an anode scaffold and a cathode scaffold, the anode scaffold is prepared from a zinc-containing hydrogel, and the cathode scaffold is prepared from an iron-containing hydrogel; the anode scaffold further comprises a prepolymerization solution prepared from dopamine, ammonium persulfate, N,N'-methylene bisacrylamide, acrylamide and tetramethylethylenediamine; the hydrogel scaffold can gradually degrade to release metal ions, play an anti-inflammatory and angiogenic role, can remove free radicals such as active oxygen and active nitrogen, and play an antioxidant role; the output voltage can be stabilized for more than 7 days, the expression of repair-related genes can be improved through physiological micro-current stimulation, the proliferation and migration of fibroblasts can be promoted, the wound can be accelerated to realize scarless healing within 16 days, and the hydrogel scaffold has a wide application prospect.

The application discloses a hydrogel support for urethral wound repair and healing, which comprises an antifouling surface layer, a ZEIN middle layer and a PNIPAM hydrogel bottom layer, wherein the antifouling surface layer is a cross-linked layer added with an antibacterial agent, and the PNIPAM hydrogel bottom layer is loaded with a repair drug. The application utilizes the characteristics that the temperature of urine is higher than that of the urethra, utilizes the temperature-sensitive characteristics of poly (N-isopropyl acrylamide), enables the drug loaded in the hydrogel to be released when the urine passes, is used for offsetting the negative effects caused by the tension change, and utilizes the synergistic effect of the three layers to accelerate the repair and healing of the urethral wound.

This invention belongs to the field of skin-like tissue preparation technology, and relates to a method for constructing ordered skin-like tissue with magnetic field assistance. The method involves directional cryo-casting to prepare a layered ordered hydrogel, providing skin-like seed cells, and culturing and expanding these cells in large quantities using a complete culture medium to obtain skin-like cells. The cultured skin-like cells are then co-cultured with iron oxide nanoparticles to prepare magnetized skin-like cells. These magnetized skin-like cells are then co-cultured with the layered ordered hydrogel, and magnetic fields are applied to both sides to induce the magnetized skin-like cells to enter the layered ordered hydrogel, resulting in a hydrogel scaffold. After washing the obtained hydrogel scaffold, it is transferred to a complete culture medium, and keratinocytes are seeded on the surface of the hydrogel scaffold and cultured to prepare an ordered skin-like tissue. This invention can effectively regulate the migration and arrangement of fibroblasts, providing a physical basis for the arrangement of the extracellular matrix, especially collagen.

The invention relates to a living plant cell-animal cell composite scaffold as well as a preparation method and application thereof. The living plant cell-animal cell composite scaffold comprises a 3D printing hydrogel scaffold used as a muscle repair carrier and a 3D printing biological ceramic scaffold used as a bone repair carrier, wherein the 3D printing hydrogel scaffold and the 3D printing biological ceramic scaffold are assembled by simulating a muscle-skeleton structure; the hydrogel scaffold comprises a polymer matrix component, and functional plant cell components, namely diatom cells and mammalian cell components, namely muscle stem cells, which are distributed in the polymer matrix component, the biological ceramic scaffold comprises a biological ceramic scaffold matrix component and mammalian cell component bone marrow mesenchymal stem cells inoculated and loaded on the biological ceramic scaffold matrix component.

This invention discloses a method for preparing a 3D-printed hydrogel scaffold for cartilage repair, comprising: providing methacrylamide chitosan; providing 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres; obtaining a 3D-printed bio-ink for cartilage repair using methacrylamide chitosan, 2-([1,1-biphenyl]-4-ylcarbamoyl)benzoic acid@polylactic acid-glycolic acid copolymer microspheres, recombinant collagen CF-1552, and lithium magnesium silicate as main raw materials; and obtaining a 3D-printed bio-ink for cartilage repair using the 3D-printed bio-ink for cartilage repair as a raw material to obtain a 3D-printed hydrogel scaffold for cartilage repair. This 3D-printed hydrogel scaffold has advantages such as antibacterial properties and the ability to promote the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into chondrocytes, and can be used for cartilage repair, potentially bringing new curative opportunities for cartilage injury diseases.

The preparation method of the HLC-CTGF fusion protein disclosed by the invention comprises the following steps: designing a target gene synthesis and enzyme cutting site, reactivating a strain, and then sequentially carrying out expression vector construction of the fusion protein HLC-CTGF, expression of the fusion protein HLC-CTGF, purification of the fusion protein HLC-CTGF and immunoblotting of the fusion protein HLC-CTGF. The rhCol-CTGF fusion protein prepared by the preparation method disclosed by the invention is non-toxic and has good cell compatibility. The collagen can be cross-linked to prepare a hydrogel scaffold, and the collagen creates a good environment for the growth of osteoblasts by providing necessary nutrition and structural support; the CTGF is slowly released by controlling the degradation of the hydrogel, so that the defect that the expression of the CTGF in vivo has time dependence is overcome, and bone tissue repair is accelerated.

The application discloses a kind of collagen Pickering emulsion 3D printing-based cartilage regeneration scaffold and preparation method thereof.Collagen is extracted from fresh collagen extraction raw material, and purified collagen sponge is obtained, the collagen sponge is dissolved in acetic acid, and high-concentration collagen solution is obtained, then oil phase is added to the collagen solution, and high-speed emulsification is carried out using high-speed homogenizer, to obtain collagen-based Pickering emulsion, finally, computer digital modeling and low-temperature hydrogel forming equipment are used, and collagen-based Pickering emulsion is used as biomaterial ink to 3D print cartilage regeneration hydrogel scaffold, the printed scaffold is quickly frozen in liquid nitrogen, and vacuum drying is carried out, to obtain cartilage regeneration scaffold.The application develops collagen Pickering emulsion biomaterial ink with excellent printability, and through 3D printing, collagen-based cartilage regeneration scaffold with good quick customization forming property, adjustable pore structure and good biological activity is obtained.

The application discloses a customizable biphasic hydrogel scaffold, which comprises a transparent cartilage layer and a subchondral bone layer, the transparent cartilage layer comprises methacrylated type I recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated type I recombinant collagen and resveratrol-loaded bioactive glass. The application further provides a preparation method and application of the biphasic hydrogel scaffold. The biphasic hydrogel scaffold has the advantages of customization, antioxidation, anti-inflammation, high biocompatibility, promotion of wound healing and bone repair and the like.

The invention relates to a thermo-sensitive hydrogel drug sustained-release stent for jointly loading a hypotensive drug and a chemical / immunotherapy drug, which is prepared from a thermo-sensitive material and loads three drug components, namely losartan, oxaliplatin and an immune checkpoint inhibitor. The temperature-sensitive hydrogel can form gel in situ under the triggering of body temperature, and meanwhile, the acting time of the medicine on a target part is prolonged. The antihypertensive drug losartan potassium can reduce tumor interstitial substances, reduce tumor solid stress, relieve vascular compression in tumors and improve tumor hypoxia. Meanwhile, the chemotherapeutic drug oxaliplatin can induce tumor immunogenic cell death, and the tumor immunosuppressive microenvironment is effectively relieved. The immune checkpoint inhibitor can be used for accurately blocking the combination of the PD-L1 and the PD-1 and activating the T cell mediated anti-tumor immune response. According to the present invention, the potent anti-tumor immune response can be triggered, the excellent tumor inhibition effect can be achieved, the strong far-end effect can be even induced, and the growth of the far-end tumor can be effectively inhibited.

The application discloses a preparation method and application of a GO-rGO / ZnO / GelMA bio-ink material. The rGO reduced by a physical method of ultraviolet irradiation is compounded with GelMA hydrogel, and ZnO nanoparticles are introduced at the same time. The rGO and the ZnO nanoparticles synergistically act, on the one hand, improve the printing performance of the GelMA hydrogel, and on the other hand, reduce the swelling rate and degradation rate of the GelMA hydrogel support, and improve the compression modulus, biological activity, osteogenic property and antibacterial performance of the GelMA hydrogel support. Therefore, the GO-rGO / ZnO / GelMA composite bio-ink material prepared by the application has the printing performance, biological activity, osteogenic property and antibacterial property, can realize integrated and synergistic repair of osteogenesis and anti-infection, and can be widely applied to the preparation of bone repair materials. Meanwhile, the ZnO-rGO composite system can be applied to interior wall coating as an effective antibacterial agent.

The application discloses a mussel-mimic bacterial-responsive injectable hydrogel and a preparation method and application thereof, and belongs to the technical field of biological medicines. The hydrogel is composed of a mussel-mimic viscous hydrogel support and a bacterial response agent; the viscous hydrogel support is QCS-EC, which is formed by non-covalent combination of quaternary ammonium chitosan QCS and epigallocatechin gallate EGCG; and the bacterial response agent is a supramolecular nanometer cross-linking compound TEBox-B 12， which is self-assembled by hydrogen bonds and electrostatic interactions. 12 H 12 2‑ The hydrogel of the application has NIR-II photothermal and photodynamic antibacterial treatment functions, can realize efficient killing of bacteria in deep tissues, has high adhesion, good biocompatibility and injectability, is convenient and applicable, can provide a good healing environment for a wound, promotes fibroblast proliferation and migration, accelerates wound tissue regeneration and healing process, and reduces scar formation.

The invention provides degradable piezoelectric water bionic hydrogel as well as a preparation method and application thereof and a preparation method of a piezoelectric bionic hydrogel scaffold. The preparation method comprises the following steps: adjusting hydrogen bond acting force by using urea and polyhexamethylene biguanide hydrochloride so as to change the pore size and the adhesion property of the hydrogel; and the hydrogel has excellent piezoelectric property by introducing a degradable piezoelectric material of a natural source. According to the present invention, the good biocompatibility is represented, and the great potential is provided in the biomedical material aspect.

A method of printing a hydrogel scaffold is provided which includes providing a container containing an ink and a liquid that is immiscible with the ink; applying light from a light source to the ink to form a portion of the hydrogel scaffold; and applying light from a light source one or more additional times to produce one or more additional portions of the hydrogel scaffold.

Three-dimensional, biocompatible, micropatterned, composite granular hydrogel scaffold compositions are provided. Also provided are methods of their production and use.

The invention discloses a high-strength 3D printed India tree glue hydrogel stent as well as a preparation method and application thereof. The India gum is methacrylic acid esterification India gum; the preparation method comprises the following steps: S1, modifying India gum hydrogel powder by methacrylic anhydride, and dialyzing and freeze-drying to obtain methacrylic acid esterified India gum powder; s2, dissolving with PBS (Phosphate Buffer Solution) containing a photoinitiator to obtain hydrogel; s3, compounding the obtained hydrogel with the bone marrow mesenchymal stem cells, printing by a biological 3D printer, and then carrying out ultraviolet light crosslinking, so as to obtain the Indian tree hydrogel scaffold. The scaffold has the advantages of being high in strength, good in biocompatibility, high in safety and controllable in material performance, and the preparation method is low in cost, wide in raw material source and easy to operate and has the potential to be applied to the field of human tissue engineering, especially cartilage repair.

Disclosed herein are compositions comprising a hydrogel scaffold, methods of generating a hydrogel scaffold, and systems for and methods of using the hydrogel scaffold to produce biologically active molecules.

The application belongs to the technical field of tumor combined immunotherapy, and particularly relates to a double-layer hydrogel support and a preparation method and application thereof. In view of the problem that the existing drugs do not combine immunotherapy and cell therapy, the application provides a double-layer hydrogel support DLS, the outer layer of which is methacrylated gelatin GelMA, and the inner layer of which is methacrylated gelatin PR. The double-layer hydrogel support can simultaneously load gene drugs and immune cells, which not only guarantees the delivery capacity of the nano-carrier and the stability of the nucleic acid, but also maintains the activity and phenotype of the immune cells, so as to realize the simultaneous treatment of solid tumors by the gene drugs and the immune cells, kill tumor cells more efficiently and conveniently, and realize precise treatment of tumors.

The invention discloses mussel-imitating bacteria responsive injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The hydrogel is formed by compounding a mussel-imitating viscous hydrogel bracket and a bacterial response agent, the viscous hydrogel scaffold is QCS-EC (Quaternized Chitosan) and is formed by non-covalent binding of QCS (Quaternized Chitosan) and EGCG (Epigallocatechin Gallate); the bacterial response agent is a supramolecular nano cross-linked compound TEBox-B12, and is formed by self-assembly of box-shaped quaternized pyridine heterocyclic organic salt molecules TEBox and salt containing suprachaotropic liquid anions B12H12-through hydrogen bonds and electrostatic interaction. The hydrogel disclosed by the invention has NIR-II photo-thermal and photodynamic antibacterial treatment functions, and can be used for efficiently killing bacteria in deep tissues; the hydrogel also has high adhesion, good biocompatibility and injectability, is strong in convenience and applicability, can provide a good healing environment for wounds, promotes fibroblast proliferation and migration, accelerates wound tissue regeneration and healing processes, and reduces scar formation.

Embodiments relate to granular aerogel scaffolds and methods of making and using thereof. A method of forming granular aerogel scaffolds includes converting polymers or lipids to form hydrogel microparticles via a first crosslinking, assembling the hydrogel microparticles to form a granular hydrogel scaffold via a second crosslinking, and subjecting the granular hydrogel scaffold to supercritical carbon dioxide drying to form a granular aerogel scaffold.

The application provides a mechanically responsive hollow hydrogel scaffold and a preparation method and application in cell loading thereof. The shell of the hollow hydrogel scaffold is obtained by coaxial printing of a bio-ink for 3D printing, and the core is a hollow structure; wherein the bio-ink for 3D printing comprises methacrylated gelatin, N-acryloylglycineamide and nanoclay; the methacrylated gelatin, N-acryloylglycineamide and nanoclay in the shell of the hollow hydrogel scaffold form physical interpenetration, and the N-acryloylglycineamide forms intermolecular hydrogen bonds, and the methacrylated gelatin and N-acryloylglycineamide form a double crosslinking network. The hydrogel scaffold can respond to mechanical stimulation, quickly and uniformly and accurately load bioactive components (such as cells) by regulating strain and mechanical loading times, and in this process, damage to cells caused by extrusion mechanical shear force can be avoided, so that the loaded cells have high viability.