22 results about "Hydrogel scaffold" patented technology

The application discloses a customizable biphasic hydrogel scaffold, which comprises a transparent cartilage layer and a subchondral bone layer, the transparent cartilage layer comprises methacrylated type I recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated type I recombinant collagen and resveratrol-loaded bioactive glass. The application further provides a preparation method and application of the biphasic hydrogel scaffold. The biphasic hydrogel scaffold has the advantages of customization, antioxidation, anti-inflammation, high biocompatibility, promotion of wound healing and bone repair and the like.

The application discloses a preparation method and application of a GO-rGO / ZnO / GelMA bio-ink material. The rGO reduced by a physical method of ultraviolet irradiation is compounded with GelMA hydrogel, and ZnO nanoparticles are introduced at the same time. The rGO and the ZnO nanoparticles synergistically act, on the one hand, improve the printing performance of the GelMA hydrogel, and on the other hand, reduce the swelling rate and degradation rate of the GelMA hydrogel support, and improve the compression modulus, biological activity, osteogenic property and antibacterial performance of the GelMA hydrogel support. Therefore, the GO-rGO / ZnO / GelMA composite bio-ink material prepared by the application has the printing performance, biological activity, osteogenic property and antibacterial property, can realize integrated and synergistic repair of osteogenesis and anti-infection, and can be widely applied to the preparation of bone repair materials. Meanwhile, the ZnO-rGO composite system can be applied to interior wall coating as an effective antibacterial agent.

The application discloses a mussel-mimic bacterial-responsive injectable hydrogel and a preparation method and application thereof, and belongs to the technical field of biological medicines. The hydrogel is composed of a mussel-mimic viscous hydrogel support and a bacterial response agent; the viscous hydrogel support is QCS-EC, which is formed by non-covalent combination of quaternary ammonium chitosan QCS and epigallocatechin gallate EGCG; and the bacterial response agent is a supramolecular nanometer cross-linking compound TEBox-B 12， which is self-assembled by hydrogen bonds and electrostatic interactions. 12 H 12 2‑ The hydrogel of the application has NIR-II photothermal and photodynamic antibacterial treatment functions, can realize efficient killing of bacteria in deep tissues, has high adhesion, good biocompatibility and injectability, is convenient and applicable, can provide a good healing environment for a wound, promotes fibroblast proliferation and migration, accelerates wound tissue regeneration and healing process, and reduces scar formation.

This invention discloses a method and application for constructing personalized organoids for multiple myeloma based on stiffness gradient regulation, belonging to the field of biomedical technology. The method includes the following steps: S1: mixing patient bone marrow plasma with a calcium-containing crosslinking agent to form a gelatable precursor; S2: combining the gelatable precursor with a three-dimensional gelatin scaffold having a stiffness gradient (Young's modulus of 25-50 kPa) to construct a three-dimensional microenvironment simulating the stiffness gradient of bone marrow; S3: gelling the precursor to form a hydrogel-scaffold composite system encapsulating target cells; S4: seeding vascular endothelial cells onto the surface of the composite system to simulate a vascular niche structure. The organoid model for multiple myeloma constructed by this invention, based on the patient's autologous bone marrow plasma and possessing stiffness gradient characteristics, can effectively simulate the bone marrow mechanical microenvironment and preserve key components of the tumor microenvironment, supporting the long-term growth of primary tumor cells and improving the accuracy of drug sensitivity prediction.

The application relates to the field of biomedical technology, in particular to a preparation device and method of a composite hydrogel scaffold, wherein the method comprises the following steps: step one, preparation of an oxidized sodium alginate solution; step two, preparation of concentrated growth factor extraction and freeze-dried powder; step three, preparation of a methacrylic acid gelatin precursor solution; step four, preparation of a composite hydrogel solution: the methacrylic acid gelatin precursor solution and the concentrated growth factor solution are mixed in proportion, the oxidized sodium alginate solution is added, mixing is performed through a composite homogenization mechanism, a hydrogel precursor solution is prepared, light curing is performed, and a composite hydrogel scaffold is obtained; wherein the composite homogenization mechanism is used for simultaneously performing mechanical stirring and ultrasonic crushing operations on the mixed solution. Through the synergistic effect of mechanical stirring and ultrasonic, the distribution difference of central uniformity and edge aggregation is reduced, the uniform dispersion degree of functional factors in the solution is improved, and the distribution uniformity of the biological activity sites of the composite hydrogel scaffold is improved.

The application discloses a kind of hydrogel three-dimensional printing method and device, and depth fusion is in printing whole process with distributed temperature control strategy and high-throughput parameter screening mechanism.Extrusion end, by double-point temperature monitoring and nozzle heat preservation shell cooperation, quantization heat steady state time and barrier external environment interference, from source guarantee the rheological stability of hydrogel extrusion;In parameter screening end, adopt cooling iteration and double concentration monofilament array test, and lossless monitoring extracts monofilament quantitative characteristics to quickly lock optimal extrusion parameter;Subsequently combined with heterogeneous grid printing strategy and in-situ quantitative monitoring, in the multi-temperature zone platform with horizontal gradient, efficiently synchronously complete the cross screening of best forming temperature;In multilayer hydrogel scaffold forming end, utilize semi-closed slot type structure to maintain stable low-temperature microenvironment in vertical space, to inhibit heat accumulation.The application solves the common problem of temperature-sensitive hydrogel rheological instability and complex structure heat collapse, and finally realizes the high-precision fidelity forming.

This invention relates to a polyamino acid-based in-situ cartilage regeneration porous gel scaffold and its preparation method. Phenylboronic acid ester is esterified and linked with polyethylene glycol-modified Kartogenin to obtain phenylboronic acid ester-modified PEGKGN. The PEGKGN modified with phenylboronic acid ester is then grafted onto the side chains of polyamino acids with allyl polyethylene glycol to obtain a graft copolymer PLBPKA. The graft copolymer PLBPKA is blended with small molecules of Kartogenin and a silk fibroin solution to form a drug-loaded self-assembly network under shear force. Finally, the polyamino acid-based in-situ cartilage regeneration porous gel scaffold is constructed through ultraviolet light crosslinking and freeze-phase separation, which is a differentially released drug hydrogel scaffold. Compared with existing technologies, the scaffold of this invention has good biocompatibility and biocontrollable degradation, and can rapidly release drugs in inflammatory environments to promote stem cell migration; while in normal physiological environments, it slowly releases drugs to promote the differentiation of stem cells into cartilage. Therefore, it can promote in-situ regeneration of articular cartilage in the absence of exogenous stem cells.

Disclosed herein are compositions comprising a hydrogel scaffold, methods of generating a hydrogel scaffold, and systems for and methods of using the hydrogel scaffold to produce biologically active molecules.

This invention belongs to the field of nanoparticle hydrogel scaffold technology, providing an MMP-responsive nanoparticle hydrogel scaffold and its application in the treatment of fibrous bone dysplasia. The nanoparticle hydrogel scaffold is prepared by cross-linking and curing triglyceride monostearate nanoparticles, 3-aminophenylboronic acid-modified hyaluronic acid, and dopamine-modified hyaluronic acid. This invention is based on the first discovery that the concentration of matrix metalloproteinases (MMPs) around fibrous bone dysplasia lesions is a biomarker reflecting the "activity and treatment response of fibrous bone dysplasia lesions," and based on this discovery, provides an MMP-responsive nanoparticle hydrogel scaffold and its application in the treatment of fibrous bone dysplasia. This nanoparticle hydrogel scaffold can achieve perilesional drug delivery and sustained MMP-responsive drug release through injection, thereby providing a local treatment strategy for fibrous bone dysplasia.

The application provides an injectable hydrogel scaffold with piezoelectric conductivity and a preparation method, comprising the following steps: (S10) preparing a piezoelectric PLLA film through an electrospinning technology; (S20) preparing PLLA nanofilaments; (S30) preparing methacrylated gelatin and methacrylated silk fibroin; (S40) mixing the PLLA nanofilaments with the methacrylated gelatin, the methacrylated silk fibroin, ionic liquid and a photoinitiator to prepare a silk fibroin mixed hydrogel; and (S50) injecting the hydrogel scaffold into a joint or a cartilage defect gap, and constructing the hydrogel scaffold through photocuring, so that the prepared injectable hydrogel scaffold is suitable for generating stable output charges under mechanical stress or ultrasonic conditions, and is suitable for being injected into a joint or a cartilage defect gap for cartilage regeneration.

The application discloses a starch-based edible scaffold and a preparation method and application thereof, and belongs to the technical field of cell scaffold materials. The edible starch is used as a base material, and a semi-interpenetrating network (Semi-IPN) hydrogel scaffold is constructed through heat-induced self-assembly, and the scaffold has good biocompatibility and edible safety. The preparation method can accurately control the elastic modulus and mechanical properties of the scaffold, so that the scaffold is suitable for providing tensile stress stimulation for fish myoblasts. Thanks to the above-mentioned characteristics, the scaffold can not only effectively support the adhesion and proliferation of fish myoblasts, but also significantly promote the directional differentiation of the fish myoblasts, and form a biomimetic muscle tissue. The application provides an innovative and performance-controllable cell scaffold solution for producing fish culture meat products which do not need to be separated from the scaffold and can be directly eaten.

The application discloses a double-sided hydrogel scaffold for urethral repair and a preparation process thereof, and belongs to the technical field of biomaterials. The PCH@PVA scaffold is constructed, a base layer on the tissue side creates a eutrophication and guiding microenvironment for regeneration by using heparin, and a functional layer on the lumen side effectively blocks the penetration of harmful components in urine and the colonization of bacteria by a polyvinyl alcohol coating, and by optimizing the crosslinking degree of PVA, the PVA has excellent mechanical toughness and elasticity, can not only withstand the periodic expansion of the urethra without breaking, but also can transmit mechanical stimulation close to the physiological range, guide cells to functional regeneration instead of pathological fibrosis. The PCH@PVA scaffold provided by the application has excellent effects in promoting re-epithelialization, angiogenesis, inhibiting fibrosis and preventing stenosis. The application also provides a new design paradigm and theoretical basis for tissue regeneration strategies in other complex physiological microenvironments.

The application provides a high-strength bacterial mineralization biomimetic bone scaffold and a preparation method thereof, the preparation method adds mineralization bacteria in a hydrogel precursor solution, uses 3D printing to prepare a hydrogel scaffold with a fine macrostructure, and then immerses the scaffold in a mineralization bacterial culture solution containing urea and mineral salts, so that the bacteria are mineralized in situ, a three-dimensional network composed of micron-level mineralized particles is formed on a microscale, and the natural bone tissue is accurately biomimetic. The preparation method has the advantages of low energy consumption, suitability for industrial production and low economic cost. The method of the application not only significantly improves the mechanical properties of the scaffold, but also optimizes the physiological microenvironment required in the bone repair process, and provides a more ideal physiological basis for bone tissue regeneration. The prepared biomimetic bone scaffold has excellent mechanical properties, a compression modulus of 12.05 MPa, and excellent biocompatibility and osteogenic activity.

This invention relates to the fields of environmental toxicology and organoid technology, and particularly to a method for assessing the toxicity of environmental microplastic exposure based on testicular organoids. The method includes: processing waste testicular tissue into a single-cell suspension, the single-cell suspension comprising supporting cells, germ cells, and interstitial cells; seeding the single-cell suspension onto a biomimetic hydrogel scaffold for culture to obtain mature testicular organoids, which are then cultured in a target microplastic exposure solution to establish a microplastic exposure model; determining the raw values ​​of toxicity assessment indicators corresponding to the microplastic exposure model, including: morphological score data, grayscale values ​​of cell marker expression, DNA damage indicators, testosterone secretion levels, and cytotoxicity indicators; calculating a comprehensive toxicity score based on the raw values ​​of each toxicity assessment indicator, and assessing the reproductive toxicity risk level of the target microplastic exposure based on the comprehensive toxicity score. This application can improve the accuracy of assessing the reproductive toxicity risk level of microplastic exposure.

The application provides a metal-polypeptide-based hydrogel support, comprising a hydrogel formed by coordination of polypeptides and metal ions, wherein the polypeptides are Ac-(FX) n -NH2, wherein n is a natural number, Ac represents a carboxyl group, NH2 represents an amino group, (FX) n represents n FX repeating units, wherein F is a phenylalanine residue, and X is selected from a glutamic acid residue or an aspartic acid residue. The hydrogel support is formed based on coordination-mediated supramolecular self-assembly of metals and polypeptides, has stable structure and good mechanical strength, and can realize excellent antibacterial or immunotherapy effect through slow release of metal ions.

This invention discloses a bilayer hydrogel scaffold, its preparation method, and its applications, relating to the field of medical repair materials technology. The bilayer hydrogel scaffold comprises upper and lower hydrogel layers made of materials with different degradation rates and mechanical properties, both layers being loaded with nano-fat emulsions. The thickness of the upper hydrogel layer is greater than or equal to the thickness of the lower hydrogel layer. This invention utilizes the differences in degradation rates and mechanical properties of different materials to prepare a bilayer hydrogel scaffold, integrating functions such as promoting nerve repair, promoting vascular activity, and promoting bone activity. It achieves the effect of simultaneously adapting to two different pathological environments within the same implant, thereby avoiding secondary surgery for patients. This invention utilizes photocrosslinking technology to achieve integrated molding of the bilayer structure, ensuring a strong interface bond between the upper and lower layers and eliminating the risk of delamination. Simultaneously, the preparation process is controllable and highly customizable, allowing for flexible adjustment of the scaffold's size and pore structure according to the clinical defect morphology.

This invention discloses a calcium phosphate ceramic particle-methacrylated gelatin hydrogel scaffold for osteoblasts pretreated with Tideglusib to enhance osteogenic differentiation, relating to the fields of biomedical engineering and bone tissue regeneration. It comprises osteoblasts pretreated with Tideglusib and activated, biphasic calcium phosphate ceramic particles, and a methacrylated gelatin hydrogel encapsulating the osteoblasts and ceramic particles. The preparation method includes: obtaining rat osteoblasts; seeding the osteoblasts onto the biphasic calcium phosphate ceramic particles; and pretreating the seeded biphasic calcium phosphate ceramic particles with Tideglusib solution to activate the Wnt signaling pathway and promote osteogenic differentiation. This invention exhibits excellent bone defect repair capabilities, good degradability and biocompatibility, provides necessary mechanical support, and has strong clinical translational potential.

The application relates to the technical field of scaffolds, and particularly discloses a composite bioactive hydrogel scaffold as well as a preparation method and application thereof. The composite bioactive hydrogel provided by the application simultaneously comprises methacrylated hyaluronic acid and methacrylated silk fibroin, and HAMA and SFMA have significant complementarity in performance, that is, the high-viscosity characteristic of HAMA can be used as a thickening agent to improve the rheological performance of SFMA, and the strong and tough mechanical performance of SFMA can make up for the brittleness defect of HAMA, and the synergistic effect can enable the mixed application of the two to form a composite hydrogel which is more superior in performance and more extensive in application range. Furthermore, platelet-rich plasma is loaded on the basis, rich cytokines are provided, and the composite bioactive hydrogel is endowed with bioactivity.

The application belongs to the technical field of biomedical material engineering, and discloses a hydrogel support for 3D tumor sphere culture and drug screening, which is formed by four-arm polyethylene glycol maleimide, fibrinogen and thrombin through Michael addition cross-linking and biological polymerization cooperation, and has an adjustable elastic modulus of 10-1000 Pa by adjusting the proportion of each component, and the gelation is completed within 5 minutes at room temperature. The support has the biological activity of fibrin and the structural stability of polyethylene glycol, can efficiently support HCT116 cells to form high-vigor tumor spheres, accurately simulates the in-vivo tumor microenvironment, significantly improves the accuracy and repeatability of drug screening, is simple to operate and compatible with the high-throughput platform of standard well plates, and is suitable for the drug sensitivity detection of tumor personalized treatment.

This application relates to a programmable 3D / 4D printable hydrogel mesoporous scaffold with adjustable mechanical and topological properties, its preparation method, and its application, belonging to the field of food biosynthesis technology. The preparation method of the hydrogel mesoporous scaffold of this application includes the following steps: first, obtaining a printable composite hydrogel ink through a one-pot delayed double crosslinking process; then, transferring the composite hydrogel ink to an extrusion 3D printer for printing to obtain a preliminary scaffold product with a three-dimensional porous structure; finally, heat treatment to obtain the scaffold. This hydrogel scaffold possesses excellent mechanical properties, a concentrated pore size distribution, adjustable porosity, and exhibits a customizable ordered hierarchical structure and high water retention capacity; it also exhibits environmental responsiveness, and the non-uniform swelling behavior of the hydrogel mesoporous scaffold can be programmed by adjusting the gelatin concentration, making it suitable as a smart scaffold for producing cultured meat.