174 results about "Hydrogel scaffold" patented technology

The invention provides a 3D biological printing osteochondral organ based on dynamic mechanical regulation and control, and a construction method and application thereof. Compared with the prior art, the invention innovatively provides a biphasic scaffold optimization modification strategy: firstly, an osteochondral hydrogel scaffold with an accurate layered structure is prepared by adopting a 3D biological printing technology, then a cell mechanical transduction signal channel is activated by a dynamic pressure stimulation system, and cartilage / bone biphasic differentiation of stem cells is directionally regulated and controlled; and by constructing a bionic mechanical microenvironment, the obtained osteochondral organ not only keeps excellent biocompatibility, but also has gradient mechanical properties and a clear spatial layered structure (cartilage layer-bone layer), the directional cartilage formation / osteogenic differentiation efficiency of the osteochondral organ is remarkably improved, and the osteochondral organ has a good application prospect. The problems of spatial heterogeneity construction and accurate regulation and control of cell fate in osteochondral tissue engineering are effectively solved, and the method has important application value in the fields of osteochondral defect repair treatment and mechanical stimulation mechanism research.

The invention discloses a porous tantalum and hydrogel combined integrated bionic bone cartilage scaffold as well as preparation and application thereof, and belongs to the field of biomedical materials. According to the invention, a porous tantalum metal scaffold and a polysaccharide hydrogel scaffold are combined to construct the porous tantalum hydrogel biphase scaffold. Wherein the porous tantalum is used as an osteogenesis layer, and the polysaccharide hydrogel is used as a cartilage regeneration layer. The porous tantalum and the hydrogel are tightly combined in a physical cross interlocking mode and cannot fall off, and the thickness size of the two layers can be directionally regulated and controlled according to actual needs. The preparation method provided by the invention is simple and easy to implement, does not use additional chemical cross-linking agents, adhesives and the like, and is greener and safer. The biphasic stent provided by the invention has the characteristics similar to bone cartilage, and has the potential of guiding synchronous regeneration of bone and cartilage.

The invention discloses a hydrogel scaffold with multiple physical gradients. The hydrogel scaffold is prepared from the following synthetic raw materials: thermo-sensitive polyisocyanide polypeptide, silk nanofibers rich in beta lamellar structures and a conductive nanomaterial. The conductive nano material dispersion liquid and the BSNF solution are uniformly mixed according to a ratio of 1: 4; at 4 DEG C, adding a BSNF-loaded conductive nano material mixture into a PIC aqueous solution, uniformly mixing, adding into a square tank with an electrode, and applying a direct-current electric field to the hydrogel; after the electric field is removed, the temperature is raised to 25 DEG C, PIC is induced to form gel, and the conductive hydrogel stent with multiple physical gradients is obtained. The hydrogel contains multiple physical gradient structures such as rigidity, conductivity and morphology. The multi-gradient hydrogel with different patterns can be constructed by regulating and controlling the shape, the position and the electric field applying time of the electrode; the preparation method disclosed by the invention is simple, convenient to operate, simple in equipment and process requirements, low in cost and capable of realizing large-scale industrial production.

The invention discloses a biphasic hydrogel scaffold of a bionic bone cartilage matrix as well as a preparation method and application of the biphasic hydrogel scaffold, and belongs to the technical field of tissue engineering. The biphasic hydrogel scaffold of the bionic bone cartilage matrix provided by the invention comprises a hard bone matrix phase and a cartilage matrix phase. Wherein hard chitosan hydrogel with a porous structure is used as a hard bone matrix phase, and soft chitosan hydrogel is used as a cartilage matrix phase. The biphase hydrogel with the asymmetric matrix stiffness and the biochemical microenvironment can effectively release active substances in a controlled mode, the release period exceeds 21 days, the time requirement for osteochondral repair is met, and lasting support is provided for proliferation and differentiation of cells. Meanwhile, the biphase hydrogel provided by the invention has good biological safety, two matrix phases have different mechanical properties, the biphase hydrogel adapts to in-vivo complex physiological and mechanical environments, the fate of stem cells can be directionally regulated and controlled, synchronous regeneration and repair of osteochondral tissues are realized, and the biphase hydrogel has a wide application prospect.

The invention relates to the technical field of biological materials, in particular to a hydrogel scaffold material for spinal cord injury repair as well as a preparation method and application of the hydrogel scaffold material. The hydrogel scaffold material AP39 (AP39-coated LIP) for spinal cord injury repair is prepared by combining the liposome-coated AP39 (AP39-coated LIP) serving as a drug with a GelMA hydrogel scaffold, and a scaffold is provided for injured spinal cords, so that axon regeneration of injured parts is guided, mitochondria of the injured parts is targeted to release H2S, neuronal injury is relieved, and the spinal cord injury repair effect is improved. Therefore, nerve regeneration and functional recovery after spinal cord injury are promoted.

Aiming at the challenge of osteochondral defect repair, the invention provides a preparation method of a fiber-reinforced self-repairing hydrogel scaffold (SBPS). According to the method, phenylboronic acid modified sodium alginate, polyvinyl alcohol and fibroin nanofibers rich in beta-Sheet are utilized to form the hydrogel through dynamic boric acid ester bonds and hydrogen bonds. The SBPS hydrogel loaded with PDGF-BB is combined with the nanosheet containing LDH / ChS / TGF-beta3, so that multi-dimensional synergy of mechanical strengthening, sequential drug release (PDGF-BB is released in an early stage and TGF-beta3 / Mg < 2 + > is released in a later stage), ROS removal and endogenous repair is realized. The Mg < 2 + > concentration gradient effect further assists staged regulation (vascularized bone regeneration-cartilage matrix synthesis), and breaks through the limitation of a single factor. Experiments prove that the scaffold can effectively drive angiogenesis, stem cell recruitment and cartilage / bone differentiation in vivo and in vitro, and finally osteochondral regeneration with structure-function matching is achieved.

The invention provides a piezoelectric composite stent, a preparation method thereof and application of the piezoelectric composite stent in tracheal defect repair, and belongs to the technical field of tissue engineering. The preparation method of the piezoelectric composite stent comprises the following steps: (1) immersing a PLLA stent into an FF polypeptide solution to prepare a PLLA-FF stent; (2) the PLLA-FF stent is immersed in a tannic acid solution, and a PLLA-FF-TA composite stent is prepared; (3) preparing oxygen-containing PLGA (poly (lactic-co-glycolic acid)) nano microbubbles, and mixing the GelMA with the oxygen-containing PLGA nano microbubbles to obtain a Gel-NB hydrogel precursor; (4) injecting the Gel-NB hydrogel precursor into the PLLA-FF-TA composite scaffold, and carrying out ultraviolet light cross-linking on the Gel-NB hydrogel precursor and the PLLA-FF-TA composite scaffold; and carrying out ultrasonic irradiation to obtain the PLLA-FF-TA (at) Gel-Nb + US composite scaffold. The piezoelectric hydrogel stent is successfully prepared, can be well used for tracheal defect repair, has the advantages of good degradation performance, high piezoelectric performance, long-term stability and high strength, and is beneficial to clinical transformation and application.

The invention relates to a two-phase hydrogel system based on space-time gradient regulation and control as well as a preparation method and application of the two-phase hydrogel system. The biphase hydrogel system can also be expressed as a GelMA composite hydrogel scaffold of a porous structure, the whole biphase hydrogel system is of a core-shell structure, the inner layer core mineralizes SilMA hydrogel microspheres, the porous GelMA hydrogel scaffold is used as an outer layer, and the angiogenesis promoting factor dimethyl oxalaminoacetic acid, the human umbilical vein endothelial cells and the bone marrow mesenchymal stem cells are loaded on the outer layer. Wherein the inner-layer core mineralized SilMA hydrogel microspheres realize continuous release of osteogenesis signals by slowly releasing nHAp, and the outer-layer porous GelMA hydrogel stent releases DMOG by rapid degradation to realize early angiogenesis, so that space-time coupling construction of blood vessels and bone tissues is realized. Through differential degradation characteristics of inner and outer layers of hydrogel, precise time sequence cooperative regulation and control of angiogenesis and bone tissue remodeling can be realized, and the bionic property and function integration capability of a vascularized bone organ structure can be improved, so that the clinical transformation potential of the vascularized bone organ structure is improved.

The invention discloses high-strength gelatin / carbonic acid dihydrazide modified sodium alginate natural polymer hydrogel for 3D printing and a preparation method and application of the high-strength gelatin / carbonic acid dihydrazide modified sodium alginate natural polymer hydrogel. A gelatin solution and a carbonic acid dihydrazide modified sodium alginate solution are blended to obtain gelatin / carbonic acid dihydrazide modified sodium alginate ink; then, the hydrogel subjected to ink 3D printing is sequentially soaked in a CaCl2 solution and soaked in an EDC / NHS solution for cross-linking to be subjected to sequential enhancement treatment, and the high-strength natural polymer hydrogel is obtained. In addition, bioactive factors can be introduced into the 3D printing high-strength natural polymer hydrogel system, so that a bioactive hydrogel stent meeting clinical requirements is prepared. The biological ink A loaded with the growth factor for promoting corneal epithelialization and the biological ink B loaded with the histone deacetylase inhibitor are printed layer by layer through a multi-nozzle 3D printing system, so that the bioactive double-layer hydrogel scaffold can be obtained and used for corneal regeneration. The operation involved in the preparation process of the 3D printing high-strength natural polymer hydrogel is simple and convenient, and the system has high designability and universality.

The invention discloses a 3D printing PCL / nHA combined bionic glycopeptide hydrogel scaffold for infected bone defect substitution and a preparation method thereof, and belongs to the field of biomedical materials. The scaffold comprises a 3D printed PCL / nHA porous scaffold, the 3D printed PCL / nHA porous scaffold is provided with three-dimensional interconnected micro-channels, the mass percentage of nHA is 10-30wt%, the scaffold is further filled with a bionic glycopeptide hydrogel layer formed by mixing methylacryloylated gelatin, aldehyde bletilla striata polysaccharide, antibacterial peptide and a photoinitiator according to the ratio of 10: 4: 2: 1 and carrying out ultraviolet irradiation polymerization, phenyl lithium phosphate is adopted as the photoinitiator, and the bionic glycopeptide hydrogel layer is a bionic glycopeptide hydrogel layer. And 3D printing process parameters are accurately controlled. The preparation method comprises the steps of PCL / nHA porous scaffold preparation, pretreatment, finished product treatment, bionic glycopeptide hydrogel solution preparation, filling polymerization and the like. The scaffold disclosed by the invention integrates excellent mechanical properties, biocompatibility, antibacterial and anti-inflammatory capabilities and strong bone induction biological activity, provides an ideal material for repairing infected bone defects, and effectively overcomes many defects of a traditional skull repairing material.

The invention discloses antibacterial and antioxidant bionic hydrogel as well as a preparation method and application thereof. The antibacterial and antioxidant bionic hydrogel disclosed by the invention comprises a hydrogel bracket, wherein the hydrogel bracket is prepared from methacrylated quaternized chitosan, acrylated cyclodextrin, methacrylated gelatin and dopamine grafted methacrylated hyaluronic acid according to the mass ratio of (2 to 4) to (1 to 3) to (5 to 7) to (0.5 to 1.5). The antibacterial and antioxidant bionic hydrogel disclosed by the invention has a porous intercommunication structure, relatively large porosity and water absorption rate, and excellent hydrophilicity, electroactivity, antibacterial property, oxidation resistance and biocompatibility. The antibacterial and antioxidant bionic hydrogel disclosed by the invention can be used as an antibacterial material, especially used as a dressing for treating chronic infection wound surfaces.

The invention discloses a silk fibroin-hyaluronic acid temperature-sensitive bioactive hydrogel scaffold, a preparation method thereof and application of the silk fibroin-hyaluronic acid temperature-sensitive bioactive hydrogel scaffold in TBI treatment, hyaluronic acid, silk fibroin and N-isopropylacrylamide are used as raw materials to construct temperature-sensitive hydrogel, and bone marrow mesenchymal stem cells (BMSCs) and paeoniflorin (PF) are loaded to form a treatment unit. Hyaluronic acid is one of components of an extracellular matrix (ECM), has high affinity to cells, and can promote proliferation of stem cells. Silk fibroin has high biocompatibility and biodegradability and good mechanical strength and ductility / toughness, and can promote tissue repair and stem cell attachment and proliferation. N-isopropylacrylamide endows the hydrogel with the characteristic of in-situ temperature-sensitive gelling. Therefore, the bioactive hydrogel stent has the advantages of components, combines the BMSCs and the PF, overcomes the obstacle that the medicine is limited by BBB to be transferred into the brain, and is implanted into a brain injury part after a TBI surgical operation to be used for repairing and treating nerve injury.

The invention relates to an E7 peptide grafted methacrylamide karaya harlequin tree hydrogel scaffold and a preparation method thereof, the hydrogel scaffold is a comprehensive bone tissue engineering scaffold, the diameter is 140-680 nm, and the hydrogel scaffold includes but is not limited to regeneration and repair of bone defects clinically; the methacrylamide and karaya karaya are mainly used as main base materials and grafted with E7 peptide, and the finally obtained methacrylamide karaya karaya hydrogel scaffold grafted with E7 peptide has a plurality of uniformly distributed pores and has a good intercommunication microstructure. The method is simple and clear in design, the shape and the microstructure of the scaffold can be accurately controlled, the mechanical property can be adjusted according to needs, the cost is relatively low, the method is suitable for tissue repair, and a preliminary research foundation is laid for exploring a novel tissue engineering scaffold.

The invention discloses a diabetic bone defect repair hydrogel stent capable of slowly releasing exosome and a preparation method of the diabetic bone defect repair hydrogel stent. The preparation method comprises the following steps: preparing biotinylated quaternized chitosan methacrylate (QCS-MA-Biotin), formylated oxidized dextran and a biotinylated exosome (Exo-Bio), carrying out avidin bridging, and carrying out photocuring, so as to form the double-network hydrogel. The scaffold disclosed by the invention has a pH-responsive slow-release characteristic, the 28-day release rate of the exosome is less than or equal to 50%, and the activity retention rate gt; in addition, the repair of the diabetic bone defect can be obviously promoted. The method is suitable for repairing defects of skull, alveolar bone and the like.

The invention provides a self-expanding hydrogel intravascular stent and a preparation method thereof, and belongs to the technical field of medical instruments, the intravascular stent is of a hollow tubular structure, and the tube wall is of a sealed type; the intravascular stent is made of a hydrogel material. After the hydrogel stent shrinks, the size of the hydrogel stent is obviously reduced, the hydrogel stent can be delivered to a target position in a balloon intervention mode, and the hydrogel stent can stay at the target position and provide a supporting effect on the hydrogel stent due to the fact that the hydrogel stent has a self-expansion effect.

The invention provides a DNA tetrahedron-based compound as well as a preparation method and application thereof, and belongs to the field of medicines. The invention designs a bioactive composite hydrogel scaffold modified by a functionalized DNA tetrahedron compound containing BMP-2, and the functionalized BMP-2 binding peptide is stably combined with a tetrahedron framework structure with a unique three-dimensional space structure and fine programmability in a chemical click mode. And further preparing with methacrylated hyaluronic acid to obtain the composite hydrogel stent. The functional DNA tetrahedron compound can efficiently capture BMP-2, the composite hydrogel scaffold can capture endogenous BMP-2 in a targeting manner in the early stage of bone defect and stimulate stem cell osteogenic differentiation so as to promote in-situ osteogenesis, a series of complications caused by delivery of exogenous bone morphogenetic protein-2 are avoided, the in-vivo stability is poor, and the in-vivo bone morphogenetic protein-2 can be used for preparing the bone morphogenetic engineering scaffold. The method has the advantages of high safety, high cost and the like, and has a good application prospect.

The invention discloses a double-layer hydrogel stent capable of releasing medicine in time sequence as well as a preparation method and application of the double-layer hydrogel stent. The scaffold comprises an inner hydrogel and an outer hydrogel coating the inner hydrogel, the outer hydrogel is loaded with cell factors, and the cell factors have the following effects of collecting neutrophils and promoting polarization of the neutrophils into subtypes with stem cell collection; the inner layer hydrogel is loaded with neurotrophic factors. According to the hydrogel scaffold, ordered slow release of cell factors (such as IL-8) and neurotrophic factors (such as NGF) is achieved through the structural design of the inner hydrogel layer and the outer hydrogel layer, neutrophils and sensory nerves can be activated in sequence in a bone defect area, the neutrophils are collected in the defect area, stem cell homing is promoted, and osteogenesis is started; then, local sensory nerves are induced to grow in through the lagged and continuously released NGF, the sensory nerves are stimulated to play an immune regulation role, inflammation dissipation is promoted, and finally repair and regeneration of refractory bone defects are promoted.

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The invention provides a preparation method of silk fibroin hydrogel with high strength and high biocompatibility, a natural silk fibroin material is utilized, a processing method of directional freezing and heating coaxial stretching is adopted, and the heating temperature needs to be higher than the glass transition temperature of the silk fibroin material, so that the maximum tensile strength of a silk fibroin hydrogel bracket is improved; and a micro directional pore channel structure is formed, so that the mechanically enhanced hydrogel stent with good biocompatibility is finally obtained. A rotator cuff patch prepared by adopting the hydrogel scaffold can achieve the mechanical strength similar to that of rotator cuff tendons in a human body, and has good biocompatibility, and the microstructure has micron-scale directional pore channels, so that directional growth of cells on the surface and in the material is facilitated, and higher repair efficiency can be realized.

The invention discloses a preparation method of a functional bone repair hydrogel scaffold, which comprises the following steps: mixing nano attapulgite and methacrylated gelatin to form a printable emulsion system, performing three-dimensional printing on the printable emulsion system, and performing ultraviolet crosslinking to form a porous three-dimensional scaffold, and incubating the porous three-dimensional scaffold in a PBS (Phosphate Buffer Solution) containing the mesenchymal stem cell exosome and the miRNA-26a-5p and / or the miRNA-30a-5p, so that the mesenchymal stem cell exosome, the miRNA-26a-5p and / or the miRNA-30a-5p are co-loaded on the porous three-dimensional scaffold. The invention also provides a scaffold prepared by the method and application of the scaffold in biology. The scaffold can load mesenchymal stem cell exosomes and specific micro-RNA, and realizes regulation and control of immune microenvironment of injured parts and promotion of bone regeneration, so that the repair effect of large-volume bone defects is effectively improved.

The invention provides an ultrasonic stimulation response hydrogel stent as well as a preparation method and application thereof. A coaxial 3D printing technology and surface chemical modification are adopted to prepare the ultrasonic stimulation response hydrogel stent with immunoregulation and osteogenesis promotion functions. The hydrogel with the skin-core structure comprises core layer hydrogel and skin layer hydrogel, wherein the core layer hydrogel is composed of methacrylated gelatin chelated metal ions modified by a diphosphonic acid compound and a methacrylate modified polymer, and the skin layer hydrogel is composed of methacrylated gelatin modified by an immunoregulatory factor and an organic sound-sensitive agent. The ultrasonic stimulation response hydrogel stent has the advantages of ultrasonic stimulation response release, good biocompatibility, macrophage M2 type polarization regulation, angiogenesis promotion, bone regeneration promotion and the like, and has potential application prospects in the field of bone defect regeneration and repair.

The invention discloses an infectious bone defect bionic repair hydrogel stent and a preparation method and application thereof, and is characterized in that the infectious bone defect bionic repair hydrogel stent is composed of an upper antibacterial-vascularization layer simulating a periosteum structure, a middle support layer simulating a bone cortex structure and a lower regeneration-regulation layer simulating a cancellous bone structure; the upper antibacterial-vascularization layer is prepared from photoresponse CuxO / SiC heterojunction powder, the middle support layer is prepared from silk fibroin, and the lower regeneration-regulation layer is prepared from HAP (at) Cur-CNDs-Pt NPs composite nanoparticles. The multi-dimensional structure and function integration realizes the full-period regulation and control of antibiosis-barrier-regeneration, breaks through the limitation of the traditional single-function stent, and provides an integrated solution for infectious bone defect repair.

The present invention is applicable to the technical field of bone defect repair, and provides a preparation method and application of a collateral-modified Gelma hydrogel. The present invention performs collateral modification on the Gelma hydrogel by Fibrinogen, successfully improving the mechanical properties and cell adhesion of the hydrogel scaffold. At the same time, the loading of NELL‑1 protein significantly improves the scaffold's ability to repair bone defects. The Fibrinogen‑Gelma / NELL‑1 hydrogel scaffold constructed by the present invention has shown ideal mechanical properties in relevant characterizations, and has been shown to play a key role in the repair of bone tissue defects in in vitro and in vivo experiments, and has greatly promoted the expression of osteogenesis-related genes. Therefore, the present invention is expected to be applied to clinical treatments related to bone defect repair, such as maxillary sinus bone augmentation and guided bone regeneration (GBR), in the future.

The present application relates to a kind of water gel support prepared by self-assembled polypeptide and the use of support obtained therefrom, the preparation method includes: 1) self-assembled peptide sequence is linked nerve growth factor analog peptide by covalent bond to obtain bonded functional polypeptide;2) macrophage is separated and cultured, and is induced to be " alternative activation " anti-inflammatory M2 macrophage, obtain culture supernatant and carry out filtration, obtain filtered cell culture supernatant;3) filtered cell culture supernatant is mixed with bonded functional polypeptide, obtain mixed solution and adjust the concentration of the mixed solution, the bonded functional polypeptide self-assembles and forms water gel support.The present application uses M2 macrophage condition culture supernatant to construct regenerative microenvironment, combines polypeptide functional water gel support, realizes better overall interaction, and prepares the water gel support that can supplement and regulate nerve regeneration, the support can promote nerve cell regeneration behavior, provides new selection for tissue engineering biomaterials.

The invention discloses a flexible actuator and a preparation method thereof. And combining the hydrogel film with the inverse opal photonic crystal structure with PDMS (Polydimethylsiloxane) to prepare the hydrogel stent actuator capable of treating cervical stenosis. The contact angle of the prepared hydrogel film with the inverse opal photonic crystal structure can reach 90 degrees or below, and the hydrogel film has strong affinity to water on a super-hydrophobic surface. Due to the super-hydrophobicity, the thin film can be tightly attached to an air-water interface while keeping self stable floating. The cervical stent disclosed by the invention has controllable adhesiveness, adjustable form, stimulation responsiveness and drug release property, the stent can be retained at the cervical part, and the application potential of the cervical stent in the aspect of treating cervical stenosis is shown.

The invention discloses repair hydrogel as well as a preparation method and application thereof. The raw materials of the repairing hydrogel comprise 3D-ECM, 3D-EVs and sodium hyaluronate, the 3D-ECM is an epimatrix extracted by 3D culture of human dermal fibroblasts in a hydrogel scaffold, and the 3D-EVs is an epivesicle extracted by 3D culture of human stem cells in the hydrogel scaffold. The repairing hydrogel provides a good example for the application of the 3D-ECM and the 3D-EVs; the repair hydrogel is rich in more than 80 kinds of protein and polysaccharide substances beneficial to regeneration and repair of the articular cartilage, and has a good effect of promoting repair of the articular cartilage injury.

The invention relates to a preparation method of an exosome-loaded photocuring hydrogel scaffold capable of promoting bone repair and a product thereof, and relates to the technical field of biological materials and tissue engineering. According to the main thought, exosome is loaded in gelatin hydroxyapatite microspheres, then the microspheres are evenly dispersed in a three-dimensional continuous network formed by light-cured hydrogel, and a microsphere matrix and a light-cured hydrogel material are the same or different. The invention has the advantages that: the photocuring hydrogel provides macroscopic physical support and a cell adhesion skeleton; and the dispersed exosome loaded hydroxyapatite microspheres are used as an independent repository, so that protection and controllable release of active substances are realized, and meanwhile, the mechanical strength of the hydrogel is enhanced. And the structure is accurate and controllable, the composite slurry can be molded into any complex two-dimensional or three-dimensional structures through a mold or a biological 3D printing technology by utilizing the photocuring characteristic, and the personalized repair requirement is met.

The invention belongs to the technical field of biomedical material engineering, and discloses a hydrogel scaffold used for 3D tumor sphere culture and drug screening, the scaffold is formed by four-arm polyethylene glycol maleimide, fibrinogen and thrombin through Michael addition crosslinking and biopolymerization synergistically, the elastic modulus of 10-1000 Pa can be adjusted by regulating and controlling the proportion of all the components, and the hydrogel scaffold can be used for 3D tumor sphere culture and drug screening. Gel forming is completed within 5 min at the room temperature. The scaffold has the biological activity of fibrous protein and the structural stability of polyethylene glycol, can efficiently support HCT116 cells to form high-activity tumor balls, accurately simulate the in-vivo tumor microenvironment and remarkably improve the accuracy and repeatability of drug screening, is easy and convenient to operate, is compatible with a standard pore plate high-throughput platform and is suitable for drug sensitivity detection of personalized treatment of tumors.

The invention belongs to the technical field of artificial meat tissue engineering, and particularly relates to a spiral composite hydrogel scaffold as well as a preparation method and application thereof. The spiral composite hydrogel scaffold provided by the invention adopts a bionic microstructure design, and can simulate fiber arrangement and a multi-scale hierarchical structure of natural beef tissues, so that ordered distribution of muscle cells and fat cells is guided in a microscopic space. By optimizing hydrogel components and a cross-linking mode, the mechanical strength and the elasticity of the stent are controllable in a relatively large range. A three-dimensional culture environment constructed by using the scaffold can promote differentiation and synchronous maturation of beef related muscle cells and fat cells, the time of a texturization process is greatly shortened, and the fibrosis degree and marbling effect of artificial meat are improved. Compared with a traditional two-dimensional culture method or a single scaffold method, the method can efficiently obtain textured artificial meat with a bionic structure and a texture close to that of natural beef, and is suitable for the fields of artificial meat manufacturing, functional food development, tissue engineering and the like.

The invention discloses a melanin-containing multi-layer structural color hydrogel microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that a colloidal particle solution is injected into a microneedle mold, and a colloidal crystal microneedle template is obtained through a deposition method; preparing a hydrogel pre-polymerization solution from melanin and a high-molecular organic matter pre-polymerization solution, injecting the hydrogel pre-polymerization solution into a microneedle mold containing a colloidal crystal microneedle template, and curing the hydrogel pre-polymerization solution to obtain a colloidal crystal hydrogel mixed microneedle patch; after the colloidal crystal microneedle template is removed, the inverse opal structure hydrogel scaffold containing melanin is obtained; preparing a pre-gel solution of a drug-added temperature-sensitive polymer material, pouring the pre-gel solution into holes of the prepared inverse opal structure hydrogel stent, and cooling and curing to form a shell layer, thereby obtaining the hydrogel stent. The hydrogel microneedle patch provided by the invention contains an inverse opal structure bracket with photo-thermal conversion capacity and inherent immunoregulation performance and a temperature-sensitive gel outer layer loaded with drug components, and integrates multiple functions of photo-thermal response, drug release, immunoregulation, sensing and the like.