Preparation method of living cell based complex three-dimensional microchannel porous support

A porous scaffold and microchannel technology, applied in the field of biomedical engineering, can solve problems such as operability needs to be improved

Inactive Publication Date: 2012-01-11
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods still need to be improved in terms of operability, controllability, and ability to construct three-dimensional complex interconnected porous microchannel hydrogels.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for preparing a complex three-dimensional microchannel porous scaffold based on living cells includes the following steps:

[0020] In the first step, a hydrogel sol is prepared, and the hydrogel monomer and hydrogel solvent are mixed uniformly at a mass ratio of 2:100 and 1.5:100 to form two hydrogel sols with different concentrations of hydrogel monomers. , The hydrogel monomer is collagen, and the hydrogel solvent is a phosphate (PBS) buffer solution with a pH of 5.7 to 8.0;

[0021] The second step is to prepare a mixture of hydrogel sol and cell culture solution. The hydrogel sol with high monomer concentration of hydrogel prepared in the first step and the cell concentration are 10%. 10 The cells / mL cell culture solution is mixed uniformly at a volume ratio of 100:1 to form the first mixture A, and the hydrogel sol with a low concentration of hydrogel monomers and the cell concentration are 10 10 The cells / mL cell culture solution is evenly mixed at a volume ra...

Embodiment 2

[0029] A method for preparing a complex three-dimensional microchannel porous scaffold based on living cells includes the following steps:

[0030] In the first step, a hydrogel sol is prepared, and the photoinitiator, hydrogel monomer and hydrogel solvent are mixed uniformly in mass ratios of 0.5:30:100 and 0.5:15:100 to form the concentration of hydrogel monomers. Two different hydrogel sols, the photoinitiator is 2-hydroxy-2-methylpropiophenone, the hydrogel monomer is polyethylene glycol diacrylate with a molecular weight of 3400, and the hydrogel solvent is pH 5.7~8.0 phosphate (PBS) buffer solution;

[0031] The second step is to prepare a mixture of hydrogel sol and cell culture solution. The hydrogel sol with high monomer concentration of hydrogel prepared in the first step and the cell concentration are 10%. 10 The cells / mL cell culture solution is mixed uniformly at a volume ratio of 30:1 to form the first mixed solution A. The hydrogel sol with a low concentration of hyd...

Embodiment 3

[0039] A method for preparing a complex three-dimensional microchannel porous scaffold based on living cells includes the following steps:

[0040] In the first step, a hydrogel sol is prepared, and the photoinitiator, hydrogel monomer and hydrogel solvent are mixed uniformly in mass ratios of 0.5:2:100 and 0.5:1:100 to form the concentration of hydrogel monomers. Two different hydrogel sols, the photoinitiator is Yangujia 2959, the hydrogel monomer is hyaluronic acid, and the hydrogel solvent is a phosphate (PBS) buffer solution with a pH of 5.7 to 8.0;

[0041] The second step is to prepare a mixture of hydrogel sol and cell culture solution. The hydrogel sol with high monomer concentration of hydrogel prepared in the first step and the cell concentration are 10%. 10 The cells / mL cell culture solution is mixed uniformly at a volume ratio of 50:1 to form the first mixed solution A. The hydrogel sol with a low concentration of hydrogel monomers and the cell concentration are 10 10 T...

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Abstract

A preparation method of a living cell based complex three-dimensional microchannel porous support. The method comprises steps of: first, preparing two parts of hydrogel collosol with different hydrogel monomer concentrations; then preparing two mixed liquors containing the hydrogel collosol and cell culture solution; putting the two mixed liquors into two sets of different injectors of a cell printer; then printing the mixed liquors on a petri dish surface until formation of a required three-dimensional cell-laden hydrogel support; cultivating the cell-laden hydrogel support in an incubator; then soaking the cell-laden hydrogel support in lauryl sodium sulfate to complete split and kill the cells; flushing with Dulbecco's phosphate-buffered saline and deionized water successively; finally obtaining the three-dimensional porous hydrogel support with complex microchannels. According to the invention, living cells are employed as a porogenic agent; size and density of the holes are controlled through controlling of the cell concentration and culture conditions; distribution of the holes is controlled through controlling of a printing platform; and a complex and controllable microchannel structure is formed in the hydrogel support.

Description

Technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a method for preparing a complex three-dimensional microchannel porous scaffold based on living cells. Background technique [0002] Porous materials have a wide range of applications in many fields such as storage, separation, catalysis, microelectronics, and biomedicine and tissue engineering. In recent years, porous scaffold materials based on hydrogel have received more and more attention in tissue engineering. The introduction of micropores in the hydrogel can increase the specific surface area of ​​the hydrogel scaffold, which is beneficial to cell growth, tissue invasion and local blood vessel regeneration, and can greatly improve the diffusion performance of the hydrogel. For example, studies have shown that the introduction of pores into alginate hydrogels can increase the internal fluid flow rate and material transport speed by nearly three orders of magnitud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/18A61L27/20A61L27/24
Inventor 徐峰卢天健黄国友
Owner XI AN JIAOTONG UNIV
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