79 results about "Porous hydrogel" patented technology

The invention relates to 3D printing porous hydrogel based on a water-water emulsion as well as a preparation method and application of the 3D printing porous hydrogel, and relates to the technical field of biomedicine. The hydrogel is formed by photocuring crosslinking of a hydrogel precursor, the hydrogel precursor comprises a continuous phase medium and a dispersed phase medium, and the volume ratio of the continuous phase medium to the dispersed phase medium is 1: (2-5). The hydrogel provided by the invention breaks through the conventional volume ratio of a continuous phase medium to a dispersed phase medium, and breaks through the volume ratio limitation brought by a traditional water-water two-phase emulsion; the photocuring forming performance, the 3D printing forming performance, the swelling performance and the mechanical performance of the hydrogel are superior to those of existing hydrogel prepared from a high-volume continuous phase medium, so that a new technical system is provided for preparing hydrogel with higher porosity and connectivity.

The invention discloses green leaf-imitated porous hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel materials. The preparation method of the green leaf-imitated porous hydrogel comprises the following steps: mixing polyvinyl alcohol, tannic acid and a composite pigment in a solid state, and grinding to form uniformly dispersed mixed powder; then spraying a glutaraldehyde solution to the mixed powder, carrying out a cross-linking reaction at room temperature, and washing after the reaction to obtain hydrogel; soaking the hydrogel in a lithium chloride solution, and taking out the hydrogel after soaking to obtain the green leaf-imitated hydrogel with the automatic moisture absorption function; the hydrogel has a porous structure, can automatically absorb moisture and simulate a vegetation spectrum in a 400-2500nm wave band at high precision, is suitable for covering the surface of fabric or equipment so as to simulate the spectrum and thermal characteristics of vegetation, and can be widely applied to the field of infrared camouflage.

The invention discloses high-strength deformable porous hydrogel for postoperative abdominal cavity tissue effusion drainage, belongs to the technical field of medical porous hydrogel, and particularly relates to a temperature-sensitive hydrogel reaction solution obtained by adding a monomer, a cross-linking agent and an initiator into water and mixing, and freezing and polymerizing to obtain ice gel; treating the ice gel in a salting-out solution to obtain porous hydrogel; the cross-linking agent is prepared from at least one of N, N-methylene bisacrylamide, N, N-methylene bisacrylamide and polyethylene glycol diacrylate. The invention provides the high-strength deformable porous hydrogel for postoperative abdominal cavity tissue effusion drainage, which has a temperature-sensitive effect, can be dynamically controlled through electric heating, and has good mechanical properties and a good drainage effect.

The application belongs to the technical field of biomedical materials, and discloses a collagen-based double-network porous hydrogel scaffold capable of recruiting endogenous stem cells and a construction method and application thereof. The scaffold takes collagen II (Col-II) and oxidized methyl acrylated hyaluronic acid (AHAMA) as an organic phase, and is constructed into a physical / chemical double-network structure through Schiff base dynamic crosslinking and ultraviolet light-induced methacryl radical polymerization. An air-in-water emulsion template method is used to form a through-porous structure, and 5' aldehyde-modified nucleic acid aptamer Apt19S is anchored in the scaffold network to achieve specific recruitment of endogenous pluripotent stem cells. The scaffold has excellent mechanical properties, a biomimetic extracellular matrix microenvironment and a targeted stem cell recruitment function, can effectively promote the directional differentiation of stem cells into chondrocytes, avoids the immune rejection and ethical issues caused by exogenous stem cell transplantation, and is suitable for the field of articular cartilage defect repair.

The invention relates to porous hydrogel based on a photo-thermal material as well as a preparation method and application of the porous hydrogel, and relates to the technical field of hydrogel. The preparation method comprises the following steps: mixing a photo-thermal material with sodium alginate and sodium polyacrylate, adding lauryl sodium sulfate for foaming, and then performing freeze crosslinking and electrostatic self-assembly, thereby obtaining the photo-thermal composite material. The porous hydrogel based on the photo-thermal material prepared by the preparation method provided by the invention can efficiently realize interface evaporation and has excellent salt resistance and antibacterial performance, the pure water evaporation rate can reach 3.03 kg.m <-2 >. H <-1 >, the evaporation rate in 20% saline water is kept at 2.43 kg.m <-2 >. H <-1 >, and after the porous hydrogel is in dynamic contact with escherichia coli for 18 hours, macroscopic bacterial colonies do not exist, and the antibacterial rate reaches 99%; in addition, self-floating can be achieved, cost can be saved in practical application, and a new thought is provided for the interface evaporator for sea water desalination.

The present invention is in the technical field of additive manufacturing and provides a continuous method for producing planar, porous hydrogels and structures. The present invention also provides an apparatus for the continuous production of hydrogels.

The invention provides a process for preparing a plasticised superporous hydrogel material comprising subjecting initial hydrogel material to treatment with an acidic solution, an optional treatment with a monovalent metal salt solution, freeze drying and plasticisation. Optionally, the process of the invention produces moulded plasticised superporous hydrogel material bodies that have one or more through-holes formed therein. The plasticised superporous hydrogel material of the present invention may be formulated as a suitable oral dosage form for use an appetite suppressant and for use to deliver a pharmaceutical and / or nutraceutical into a human or animal body.

The present application relates to the technical field of biological tissue engineering, and particularly relates to an artificial blood vessel and a preparation method thereof. The present application creatively develops an artificial blood vessel with a double-network interpenetrating structure based on a design of space-time coupling, wherein a double-layered openwork scaffold serves as a space maintenance network, and a porous hydrogel tube wall serves as a temporary filling network, so that accurate space-time adaptation of degradation and regeneration is realized. Specifically, the double-layered openwork scaffold serves as a main load-bearing framework, and the slow degradation characteristic of the double-layered openwork scaffold provides independent, stable and long-term mechanical support, thereby fundamentally avoiding premature degradation of mechanical properties. Meanwhile, the fast-degrading hydrogel tube wall ensures the airtightness of the blood vessel in the early stage after implantation, and then degrades rapidly to leave a preset and through three-dimensional space for tissue regeneration, thereby ensuring the speed and integrity of the regeneration.

Described herein are systems, methods, and interconnecting porous hydrogel blocks for ex vivo kidney tissue generation. A block may comprise a 3D continuous polymeric matrix with a network of microporous cavities, and may be configured to interconnect with at least one other block. A system may comprise at least one block and be configured to cultivate one or more kidney-like organ components when seeded with at least one HEK293 cell and at least one kidney cell isolated from at least one specimen. A method may comprise seeding at least one block with at least one HEK293 cell and at least one kidney cell isolated from at least one specimen

According to the graded photo-thermal-gradient needle tip array type saline-alkali soil evaporator and the preparation method thereof, cork wood-derived cellulose is used as a matrix raw material, a graded photo-thermal layer of carbon nano tubes and biochar is constructed, a gradient-size needle tip array is designed, and a porous hydrogel matrix structure is optimized, so that the graded photo-thermal-gradient needle tip array type saline-alkali soil evaporator is obtained. And the evaporator with the functions of efficient photo-thermal conversion, directional water transmission and thorough salt falling is prepared. The full-spectrum absorption efficiency of the prepared evaporator is larger than or equal to 95%, the salt crystallization falling rate is larger than or equal to 98%, the evaporation rate reaches 1.8 kg / mh under one sunlight intensity, the performance degradation is smaller than or equal to 5% after 100-hour continuous operation, the problems that a traditional evaporator is low in photo-thermal efficiency, salt is left and accumulated, and the water transmission resistance is large can be effectively solved, and the evaporator is suitable for saline-alkali soil treatment, fresh water generation and mineral salt collection scenes.

Described herein are systems, methods, and interconnecting porous hydrogel blocks for cell culture. A block may comprise a 3D continuous polymeric matrix with a network of microporous cavities, and may be configured to interconnect with at least one other block. A system may comprise at least one block and be configured to be perfused via at least one cavity by at least one perfusate under one or more controllable flow rates, pressures, volumes, viscosities, and / or osmotic pressures. A method may comprise exposing at least one block to at least one perfusate under one or more controllable flow rates, pressures, volumes, viscosities, and / or osmotic pressures.

The invention discloses an expandable self-adaptive bionic tissue repair nail. The expandable self-adaptive bionic tissue repair nail comprises a nail cap and a nail body which are made of biodegradable materials, the nail cap is a double-layer film layer, and the layer, away from the nail body, of the nail cap is a smooth compact layer. The layer, close to the nail body, of the nail cap is a porous support layer, and the surface of the porous support layer is integrated with a combining mechanism tightly attached to the tissue surface. The nail body is of a hollow net-shaped structure, and the inner cavity of the nail body is filled with swellable and biodegradable porous hydrogel; the nail body can be in a compressed and folded compact state in a dehydration state and is expanded and unfolded after encountering water; drugs are loaded in the porous hydrogel. Before implantation, the repair nail is in a dehydrated slender state; after implantation, the internal hydrogel swells when encountering liquid, an irregular fistula channel is filled with the net-shaped structure in a self-adaptive mode, meanwhile, an inner opening is sealed by the nail cap, physical bacteria blocking is achieved, and the nail cap serves as a regeneration support to actively promote repair. The device can be implanted in a minimally invasive mode, can expand in a self-adaptive mode after operation so as to be tightly attached to tissue, and meanwhile has the anti-infection function and the active repair promoting function.

The invention discloses an application of gelatinized intestinal epithelial cells in preparation of drugs for treating inflammatory bowel disease, the gelatinized intestinal epithelial cells take mouse intestinal epithelial cells as cores, poly (ethylene glycol) diacrylate, a photoinitiator and poly-L-lysine permeate into the cells through low-temperature freeze thawing, and an intracellular porous hydrogel skeleton is formed through ultraviolet crosslinking. The gelated intestinal epithelial cell has a strong cfDNA and inflammatory factor removal function, has good biocompatibility, has no direct or indirect toxic effect on a human body, has no potential toxicity, and can be used for treating inflammatory bowel diseases.

This invention discloses a method for preparing a colorimetric sensing gel for formaldehyde detection, and the corresponding colorimetric sensing gel. The preparation method includes the following steps: (1) grafting aldehyde groups; (2) grafting sensing molecules; (3) preparation of porous gel and core-shell structure. This invention uses chemical doping to graft amino-rich polyamine sensing molecules onto green and renewable calcium alginate to prepare a low-cost, green porous hydrogel for detecting formaldehyde concentration in the air.

The invention discloses a preparation method and application of a flexible supercapacitor electrode material. A porous hydrogel matrix with a semi-interpenetrating network structure is constructed by using carboxymethyl nanocellulose and sodium alginate, and a conductive polymer polypyrrole PPy is assembled by using a one-step oxidation in-situ polymerization reaction, so that a hydrogel supercapacitor electrode material with high conductivity and high area specific capacitance is successfully prepared. The method is simple to operate and low in cost, and the used raw materials are green and environment-friendly. The electrode material has good structural stability and excellent electrochemical performance, and can be directly stacked in combination with a carbon cloth current collector and filter paper without a cross-linking agent after being soaked in an electrolyte to be applied to a high-performance all-solid-state supercapacitor.

The invention provides a bilayer active biological aerosol sampling membrane of a bionic peritoneum and a preparation method of the bilayer active biological aerosol sampling membrane of the bionic peritoneum, the sampling membrane is formed by compounding a functional capture layer and an activity maintaining base layer through interface crosslinking, and the functional capture layer is composed of a hydrophilic modified polypropylene porous film. The activity maintaining base layer is composed of porous hydrogel loaded with nutrient substances. The preparation method comprises the following steps: preparing the functional capture layer; preparing an activity maintaining base layer; and compounding. Through the bionic peritoneum structural design, the bioaerosol can be efficiently intercepted, and meanwhile, the activity of microorganisms in the bioaerosol can be maintained for a long time.

Described herein are systems, methods, and interconnecting porous hydrogel blocks for co-cultivation of a plurality of cell types. A block may comprise a 3D continuous polymeric matrix with a network of microporous cavities, and may be configured to interconnect with at least one other block. A system may comprise at least one block and be configured to co- cultivate the cell types when seeded with at least one cell of each type. A method may comprise seeding at least one block with at least one cell of each type.

Porous hydrogel microspheres and the preparation method are provided thereof, the method comprising: mixing a gelation phase material and a cell-loaded pore-forming phase material at a temperature of 5-15° C. using a droplet microfluidic chip, and forming first droplets through a shear force; the cell-loaded pore-forming phase material includes a pore-forming phase material and cells, the gelation phase material including gelatin methacryloyl, polyethylene glycol diacrylate, and a photoinitiator, and the pore-forming phase material including polyethylene oxide and gelatin; then shearing a droplet stream formed by the first droplets through an oil phase material to form second droplets; curing the second droplets by an ultraviolet light irradiation to form hydrogel microspheres, and removing the pore-forming phase material from the hydrogel microspheres to obtain porous hydrogel microspheres. The porous hydrogel microspheres with stable morphology can be prepared, and the porous structure of the porous hydrogel microspheres is conducive to cell proliferation and expansion.

Described herein are systems, methods, and interconnecting porous hydrogel blocks for generating mammalian tissue. A block may comprise a three-dimensional continuous polymeric matrix with a network of microporous cavities, and may be configured to interconnect with at least one other block. A system may comprise a plurality of blocks configured to interconnect to form a scaffold configured to cultivate one or more cell types organized into stratified layers. A method may comprise acquiring a plurality of blocks, interconnecting the blocks to form a scaffold configured to cultivate one or more cell types organized into stratified layers, and seeding at least one block with at least one cell type adherent to the block. Embodiments may comprise perfusing at least one block with culture media and / or harvesting mammalian tissue generated.

The invention belongs to the technical field of medical materials, and particularly relates to a preparation method of medical high-biocompatibility porous hydrogel, and the preparation method comprises preparation of a hydrogel photocuring system, coating and Faraday reaction. In the Faraday reaction process, sodium ions or potassium ions in the hydrogel sheet move towards the cathode to form a concentration difference in the hydrogel, chloride ions and hydrogen ions in the hydrogel sheet generate a large amount of gas at the same time, bubbles are generated in the hydrogel by utilizing the gas or substance concentration change generated by the Faraday reaction, and the bubbles are uniformly distributed; therefore, a uniform aperture structure is formed. According to the method, the cytotoxicity is remarkably reduced, in the Faraday reaction process, high-activity oxidation and reduction generated by the electrode can be subjected to rapid and efficient chemical reaction with unpolymerized monomers in uv polymerization, polymerization is promoted to be initiated again, and the situation that the residual monomers are adverse to wounds is avoided. The porous hydrogel prepared by the invention is verified by an MTT (Methyl Thiazolyl Tetrazolium) method cytotoxicity test method, and the relative proliferation rate of cells is greater than or equal to 90%.

Disclosed in the present invention are a frameless electric motor based on a porous hydrogel, and a heat dissipation method therefor. A front sealing shell and a rear sealing shell are respectively mounted at a front end and a rear end of a motor stator, and the front sealing shell, the motor stator and the rear sealing shell are all annular; a motor rotor is arranged on the inner side of the motor stator, the front sealing shell mates with one end of the motor stator in an embedded manner to form a plurality of axially distributed slots, and a hot-end hydrogel is provided inside the slots; the rear sealing shell and the other end of the motor stator are embedded, an annular cold-end hydrogel is provided inside the rear sealing shell close to the rear end of the motor stator, the cold-end hydrogel is in contact with the hot-end hydrogel, and an inner graphene heat-conducting strip and an outer graphene heat-conducting strip are respectively provided on the inner side and the outer side of the annular cold-end hydrogel; and the hot-end hydrogel and the cold-end hydrogel are both provided with cavities which are in communication with each other, and are internally filled with a cooling liquid. The heat dissipation solution provided by the present invention has a simple structure and is easy to integrate. The manufacturing of a capillary structure closely fitting against a heat source of an electric motor is realized by means of the hydrogel. The porous hydrogel has high thermal conductivity and a simple preparation method.

Described herein are systems, methods, and interconnecting porous hydrogel blocks for generating personalized microvasculature ex vivo. A block may be configured to, under dynamic conditions, cultivate microvascular formation when seeded with at least one line of endothelial cells isolated from at least one first specimen and at least one line of mesenchymal stem cells isolated from at least one second specimen. A system may comprise at least one block so configured. A method may comprise seeding at least one block with at least one line of endothelial cells isolated from at least one first specimen and at least one line of mesenchymal stem cells isolated from at least one second specimen and exposing the cell types to dynamic culture conditions.

This invention discloses a flame-retardant, tough, conductive, porous hydrogel, its preparation method, and its applications, belonging to the field of hydrogel materials technology. The method involves solid-state mixing of polyvinyl alcohol and tannic acid, liquid-phase preparation of MXene and glutaraldehyde dispersions, followed by solid-liquid crosslinking, washing, and then composite with silver paste, carbon fiber cloth, and a PDMS membrane to obtain a composite material. Key parameters such as the amount of MXene added and the crosslinking time were optimized. The resulting hydrogel has a porous structure and exhibits excellent flame retardancy, toughness, and hygroscopicity. It can generate a water voltaic voltage during moisture absorption and dripping. The composite structure further enhances its power generation performance, and its electrical output is modulated by temperature and humidity, dripping volume, solution ion concentration, and polarity. This invention solves the problems of short battery life, mechanical mismatch, and poor air permeability in traditional smart textile materials, and has promising application prospects in smart textile fields such as fabric weaving and the manufacture of new sensors.

The application discloses a kind of high-strength deformable porous hydrogel for postoperative abdominal cavity tissue effusion drainage, belong to medical porous hydrogel technical field, specifically related to monomer, crosslinking agent and initiator are added to water and mixed to obtain the reaction solution of temperature-sensitive hydrogel, freeze polymerization obtains ice gel;Ice gel is placed in salt solution and is handled to obtain porous hydrogel;Monomer includes at least one of N-isopropyl acrylamide, N-vinyl caprolactam, N,N-diethyl acrylamide, N-vinyl carbazole, acrylic acid, methyl methacrylate, ethylene glycol methyl ether acrylate, butyl methacrylate, maleic anhydride and styrene.The application provides a kind of temperature-sensitive effect, can be dynamically controlled by electric heating, good mechanical property, good drainage effect high-strength deformable porous hydrogel for postoperative abdominal cavity tissue effusion drainage.

The invention relates to regenerated cartilage based on hydrostatic pressure stimulation and a preparation method and application thereof. The method comprises the following steps: inoculating cells on a porous hydrogel scaffold, placing the porous hydrogel scaffold in a culture medium, performing pressure stimulation by adopting a hydrostatic pressure bioreactor, and performing in-vitro culture for a period of time to obtain the regenerated cartilage based on hydrostatic pressure stimulation. Key mechanical sensitive channels TRPV4 and PIEZO1 are up-regulated through hydrostatic pressure stimulation, a Ca < 2 + > dependent TRPV4 / PIEZO1-Ca < 2 + >-SOX9 mechanical transduction pathway is activated, cartilage formation is promoted, and hypertrophy and ossification are inhibited. Through verification, the bioreactor system is combined with the porous hydrogel scaffold, has good biocompatibility, controllable pore structure and mechanical property, and is suitable for cartilage cell culture. After in-vitro pre-culture for 4 weeks, the cartilage-like construct subjected to HP pretreatment shows a long-term stable regeneration effect in an animal model (such as a goat), and especially in an in-situ auricle microenvironment, inflammation and heterotopic ossification can be effectively inhibited, and an efficient in-vitro cartilage engineering strategy is provided for repairing of various cartilage injuries.

The invention belongs to the field of water treatment, particularly relates to solar energy treatment of volatile phenol wastewater, and particularly relates to amphiphilic porous hydrogel as well as a preparation method and application thereof. Specifically, an amphiphilic polymer, polyvinyl alcohol serving as a base material and a carbon nano tube serving as a photo-thermal material are subjected to system structure adjustment through a Pickering emulsion template method; and carrying out crosslinking in a physical or / and chemical manner to obtain the amphiphilic porous hydrogel. The prepared hydrogel is applied to a solar device to treat volatile phenol wastewater. According to the solar evaporator, by means of the porous structure and the chemical property of the solar evaporator, the excellent water evaporation rate and the efficient photo-thermal conversion efficiency are achieved, and purified water is effectively extracted from phenol wastewater. The method has the advantages that the preparation process is simple and convenient, the cost is low, the method is environment-friendly, the method has wide application potential in the field of water purification, and a new idea is provided for solving the problem that VOCs are difficult to remove in the solar distilled water treatment process.

The invention discloses a composite hydrogel for repairing a bone-tendon interface as well as a preparation method and application of the composite hydrogel. The composite hydrogel comprises human mesenchymal stem cell-derived extracellular vesicles pretreated by quercetin, and a gelatin methylacrylate hydrogel carrier which is modified by alpha-lipoic acid and has a porous structure. The preparation method comprises the following steps: preparing a quercetin-pretreated small extracellular vesicle suspension; preparing a gelatin methylacryloyl compound porous hydrogel modified by alpha-lipoic acid; and the small extracellular vesicles are loaded in the porous structure of the hydrogel. The composite material can effectively inhibit local tissue cell senescence, significantly improve the mechanical strength of a bone-tendon interface after healing, realizes sustained release of bioactive components by using a porous structure, and has a good application prospect in repair of refractory tendon-bone interface injuries such as osteoporosis.

This invention discloses an injectable porous hydrogel microsphere containing magnesium and targeting cartilage, its preparation method, and its application. The preparation method is as follows: Step 1, preparing phosphorylated methacrylamide gelatin; Step 2, thoroughly mixing the product from Step 1 with magnesium oxide nanoparticles and a photoinitiator to form an aqueous phase, and then preparing MgO-GelMA-PS microspheres with an oil phase using a microfluidic device; Step 3, reacting the hydrogel microspheres from Step 2 with a chitosan quaternary ammonium salt solution to obtain M-GP@QCS. The hydrogel microspheres of this invention possess phosphate groups, which can provide binding sites to form stable metal ligand supramolecular bonds with MgO NPs, achieving continuous release of magnesium ions and long-term therapeutic effects on osteoarthritis; a chitosan quaternary ammonium salt coating is constructed on the surface of the microspheres, and the positive charge of the coating binds to the negatively charged cartilage tissue, achieving targeted adhesion of the microspheres to cartilage and realizing precise treatment of osteoarthritis.

The invention provides an oligonucleotide hydrogel micro-nano gene compound as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: firstly, designing C5ASO, and loading the C5ASO on a nano-carrier modified with HAP-1 peptide (targeting fibroblast-like synovial cells) and active ester; meanwhile, the porous HAMA hydrogel microspheres are prepared by utilizing a photo-crosslinking micro-fluidic technology; then, DBCO-TIMP and an azide group are respectively introduced into the liposome and the hydrogel microspheres through Michael addition and an amide coupling reaction, so that a micro-nano gene compound (C5ASO (at) HAP-CL-TIMP (at) HMs) is formed; in addition, the liposome and the hydrogel microspheres are efficiently compounded through click chemistry. The hydrogel micro-nano gene compound not only can efficiently load ASO lipidosome, but also can enhance the stability of a gene therapeutic agent in an in-vivo delivery process. Meanwhile, the MMP-9 response peptide structure in the RA articular cavity inflammation microenvironment can be accurately activated, so that continuous complement intervention is realized.

The invention relates to hydrogel based on an ice skeleton and a preparation method thereof, in the method, the ice skeleton is used as a template guide, a 30wt% PVA solution is poured and filled into the ice skeleton at the temperature of-4 DEG C to-6 DEG C, the ice skeleton is frozen at the temperature of-18 DEG C to-22 DEG C, then the ice skeleton is dissolved and removed at the temperature of 20 DEG C to 30 DEG C, and the hydrogel with a preset three-dimensional directional microstructure is obtained. According to the preparation method, an ice skeleton is used as a process route of template guiding, PVA solution filling, freezing shaping and ice melting demolding, the obtained hydrogel has a regular three-dimensional directional microstructure, the mechanical strength and the structural stability of the hydrogel are superior to those of random porous hydrogel, the technical problem that in a traditional method, biotoxicity residues exist or demolding is difficult is solved, and the preparation method is suitable for large-scale industrial production. The high biocompatibility, the high integrity and the accurate regulation and control of the orientation and the size of the hydrogel are realized.