123 results about "Porous hydrogel" patented technology

The invention provides a device and a method for extracting water from air operated under all working conditions. The device comprises a solar power supply unit, a salt-carrying hydrogel adsorption-desorption unit, an electric heating desorption unit, a water vapor condensation and collection unit and a PID control unit. The solar power supply system comprises a solar photovoltaic panel, an MPPT (maximum power point tracking) type photovoltaic inverter and a storage battery, the salt-carrying hydrogel adsorption-desorption unit comprises hydrophilic porous hydrogel and a LiCl / CaCl2 composite adsorption material, and the electric heating desorption unit comprises a copper heat-conducting plate loaded with the adsorption material and an electric heating sheet. The water vapor condensation and collection unit is composed of four finned radiators and two semiconductor chilling plates. The PID control unit is composed of a temperature and humidity sensor and a control circuit, wherein the temperature and humidity sensor is connected to the radiators and the front side of the adsorption bed. Water vapor in the environment is enriched through an efficient salt-loaded adsorbent packed bed, desorption is driven in two modes of solar photo-thermal and electric heating, desorbed high-humidity airflow passes through a fin type cooling fin connected with a semiconductor and then is condensed into liquid water, and the process of taking water from air is completed through a collector below. According to the device, low-energy-consumption air water taking under the humidity of 15%-100% can be achieved by controlling the operation time of all the units, the device is portable and compact, the automation degree is high, and high-water-yield air water taking is achieved by using solar photo-thermal and power generation as energy input.

The invention belongs to the technical field of water treatment, and particularly relates to a solar water evaporator and a preparation method and application thereof. According to the solar water evaporator provided by the invention, by using the carbon-based photo-thermal functional materials such as the carbon nanotubes, the micron porous hydrogel and the porous salt-inhibiting mineral powder, an efficient water transmission path and a steam escape path can be provided so as to improve the evaporation efficiency of the solar water evaporator; the porous salt inhibition mineral powder such as diatomite is doped to realize the porous physical salt inhibition mechanism and the surface charge repulsion effect of the mineral powder to effectively inhibit the salt, in addition, through the design of the conical structure with the height-diameter ratio being equal to 1.25, the automatic falling of the surface salt is effectively promoted through the gravity effect, the condition that the salt crystals are difficult to block the pore channels is reduced, and the service life is prolonged. And the evaporation rate stability of the solar water evaporator is improved, so that the technical problem of lack of a high-performance solar water evaporator in the prior art is solved.

The invention relates to a two-phase hydrogel system based on space-time gradient regulation and control as well as a preparation method and application of the two-phase hydrogel system. The biphase hydrogel system can also be expressed as a GelMA composite hydrogel scaffold of a porous structure, the whole biphase hydrogel system is of a core-shell structure, the inner layer core mineralizes SilMA hydrogel microspheres, the porous GelMA hydrogel scaffold is used as an outer layer, and the angiogenesis promoting factor dimethyl oxalaminoacetic acid, the human umbilical vein endothelial cells and the bone marrow mesenchymal stem cells are loaded on the outer layer. Wherein the inner-layer core mineralized SilMA hydrogel microspheres realize continuous release of osteogenesis signals by slowly releasing nHAp, and the outer-layer porous GelMA hydrogel stent releases DMOG by rapid degradation to realize early angiogenesis, so that space-time coupling construction of blood vessels and bone tissues is realized. Through differential degradation characteristics of inner and outer layers of hydrogel, precise time sequence cooperative regulation and control of angiogenesis and bone tissue remodeling can be realized, and the bionic property and function integration capability of a vascularized bone organ structure can be improved, so that the clinical transformation potential of the vascularized bone organ structure is improved.

The invention discloses preparation and application of a biological material for targeted therapy of osteoarthritis, and the preparation method comprises the following steps: according to a phage screening principle, after phages are combined with cartilage cells, washing off phages which are not combined with the cartilage cells, are not firmly combined with the cartilage cells and are non-specifically combined with the cartilage cells; eluting the specifically bound bacteriophage with glycine acid; after the specific binding bacteriophage is amplified by escherichia coli, screening is carried out by utilizing a bacteriophage display technology and cartilage cells; the preparation method comprises the following steps: preparing a small-size porous microgel loaded drug by adopting a multiple Pickering emulsion template method; and grafting the screened cartilage targeting peptide to the surface of the porous hydrogel microsphere to obtain the HS-SP microsphere. According to the invention, the problem of OA targeting can be effectively solved by adopting a phage display technology, and a drug is precisely targeted and delivered to an OA lesion region; the porous hydrogel microspheres with small particle size are prepared by adopting a multiple Pickering emulsion method, so that the use of a large number of surfactants is avoided, and the efficient loading and release of drugs can be realized.

The invention relates to 3D printing porous hydrogel based on a water-water emulsion as well as a preparation method and application of the 3D printing porous hydrogel, and relates to the technical field of biomedicine. The hydrogel is formed by photocuring crosslinking of a hydrogel precursor, the hydrogel precursor comprises a continuous phase medium and a dispersed phase medium, and the volume ratio of the continuous phase medium to the dispersed phase medium is 1: (2-5). The hydrogel provided by the invention breaks through the conventional volume ratio of a continuous phase medium to a dispersed phase medium, and breaks through the volume ratio limitation brought by a traditional water-water two-phase emulsion; the photocuring forming performance, the 3D printing forming performance, the swelling performance and the mechanical performance of the hydrogel are superior to those of existing hydrogel prepared from a high-volume continuous phase medium, so that a new technical system is provided for preparing hydrogel with higher porosity and connectivity.

The invention discloses green leaf-imitated porous hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel materials. The preparation method of the green leaf-imitated porous hydrogel comprises the following steps: mixing polyvinyl alcohol, tannic acid and a composite pigment in a solid state, and grinding to form uniformly dispersed mixed powder; then spraying a glutaraldehyde solution to the mixed powder, carrying out a cross-linking reaction at room temperature, and washing after the reaction to obtain hydrogel; soaking the hydrogel in a lithium chloride solution, and taking out the hydrogel after soaking to obtain the green leaf-imitated hydrogel with the automatic moisture absorption function; the hydrogel has a porous structure, can automatically absorb moisture and simulate a vegetation spectrum in a 400-2500nm wave band at high precision, is suitable for covering the surface of fabric or equipment so as to simulate the spectrum and thermal characteristics of vegetation, and can be widely applied to the field of infrared camouflage.

The invention discloses high-strength deformable porous hydrogel for postoperative abdominal cavity tissue effusion drainage, belongs to the technical field of medical porous hydrogel, and particularly relates to a temperature-sensitive hydrogel reaction solution obtained by adding a monomer, a cross-linking agent and an initiator into water and mixing, and freezing and polymerizing to obtain ice gel; treating the ice gel in a salting-out solution to obtain porous hydrogel; the cross-linking agent is prepared from at least one of N, N-methylene bisacrylamide, N, N-methylene bisacrylamide and polyethylene glycol diacrylate. The invention provides the high-strength deformable porous hydrogel for postoperative abdominal cavity tissue effusion drainage, which has a temperature-sensitive effect, can be dynamically controlled through electric heating, and has good mechanical properties and a good drainage effect.

The invention discloses a method for realizing in-vitro amplification of adherent cells by utilizing a stirring type bioreactor matched with a three-dimensional porous hydrogel carrier. The method comprises the following steps: sterilizing a hydrogel carrier and a stirred bioreactor, adding a cell culture medium, ventilating, inoculating cells, starting the stirred bioreactor for culturing, observing the color change of a culture solution in the culture process, and changing the solution when the culture solution turns yellow, thereby ensuring the nutrition required by cell culture. The hydrogel with high biocompatibility is used as a carrier, a porous three-dimensional growth environment is provided, cells can be attached to the interior of the hydrogel carrier, the cell growth space is sufficient, the cells can be cultured for more generations, the number and density are greatly increased, the amplification multiple can reach 10 times or above, the production efficiency is improved, and the production cost is reduced.

Disclosed are a bio-ink for promoting microangiogenesis, a method for preparing same, and use thereof. The bio-ink is prepared by the following method: 1) preparing a filamentous microgel with a diameter within 20 microns from a sacrificial-phase material A as a porogenic template; 2) seeding vascular endothelial cells and perivascular cells on the surface of the filamentous microgel to give a hydrogel microfilament with the surface loaded with cells; and 3) mixing the hydrogel microfilament with the surface loaded with cells with a solution of a matrix-phase material B to give the bio-ink for promoting microangiogenesis. The bioprinting using the bio-ink can construct a capillary-like structure and cross-scale microvessels. The present invention can achieve the preparation of a micro-channel porous hydrogel structure with an average diameter of 20 microns or less and controllable porosity and the formation of a capillary-like network structure.

The present disclosure provides a preparation method of a porous hydrogel adsorbent based on Radix astragali residues, including the following steps: subjecting residues of Chinese herbal medicine Radix astragali as a precursor to bleaching with NaClO2, alkaline washing with KOH, and high power ultrasonic treatment, thereby obtaining a precursor solution of uniformly dispersed cellulose nanofibers (CNFs); adding the precursor solution of CNFs to a mixed solution of N,N′-methylene bisacrylamide (MBA), acrylic acid (AA) and ammonium persulfate (APS), shaking evenly, and initiating a polymerization reaction at a predetermined temperature to form a monolithic gel; and cleaning the monolithic gel, putting the cleaned monolithic gel into a dimethyl sulfoxide (DMSO) solution containing epichlorohydrin to allow reaction, and transferring the product of the reaction to an aqueous sodium hydroxide solution containing triethylene tetramine to allow reaction, thereby finally obtaining an amino-functionalized porous hydrogel adsorbent.

The invention provides a method for preparing oriented porous hydrogel by using a salt crystallization template, which comprises the following steps of: mixing salt and hydrogel monomers, cooling to obtain a supersaturated salt hydrogel solution, placing a crystal nucleus template in the solution to guide nucleation and growth of salt crystals, and finally curing and cleaning the hydrogel to obtain the oriented porous hydrogel. The prepared hydrogel is anisotropic, highly ordered, controllable in pore structure size and high in biocompatibility. The preparation method has the characteristics of simple equipment, low cost, simplicity, convenience and easiness in operation, and the hydrogel can be selected from a wide variety of preparation materials and has a wide application prospect in the field of tissue engineering.

The present application provides a kind of fresh-cut tuber vegetables' hydrogel spontaneous modified atmosphere coating preservative and preservation method, belongs to the technical field of preservative.The fresh-cut tuber vegetables' hydrogel spontaneous modified atmosphere coating preservative of the present application includes hydrogel particle, film-forming base and edible plasticizer, the mass addition amount of the hydrogel particle in film-forming base is 0.01% to 3.00%, film-forming base is the chitosan solution with mass concentration of 0.2% to 5%, and edible plasticizer includes one or more of gelatin, glycerol or polyethylene glycol.The present application adds loose porous hydrogel into film-forming base solution to prepare preservative, and the preservative is coated on fresh-cut tuber vegetables such as lotus root to form a layer of edible film on the surface of fresh-cut tuber vegetables, the film uses hydrogel particle as atmosphere " switch " to control the microenvironment of the atmosphere around the vegetables, reduce water loss, thereby reducing the browning and water loss of fresh-cut tuber vegetables, and prolonging the shelf life.

The application belongs to the technical field of biomedical materials, and discloses a collagen-based double-network porous hydrogel scaffold capable of recruiting endogenous stem cells and a construction method and application thereof. The scaffold takes collagen II (Col-II) and oxidized methyl acrylated hyaluronic acid (AHAMA) as an organic phase, and is constructed into a physical / chemical double-network structure through Schiff base dynamic crosslinking and ultraviolet light-induced methacryl radical polymerization. An air-in-water emulsion template method is used to form a through-porous structure, and 5' aldehyde-modified nucleic acid aptamer Apt19S is anchored in the scaffold network to achieve specific recruitment of endogenous pluripotent stem cells. The scaffold has excellent mechanical properties, a biomimetic extracellular matrix microenvironment and a targeted stem cell recruitment function, can effectively promote the directional differentiation of stem cells into chondrocytes, avoids the immune rejection and ethical issues caused by exogenous stem cell transplantation, and is suitable for the field of articular cartilage defect repair.

The invention relates to porous hydrogel based on a photo-thermal material as well as a preparation method and application of the porous hydrogel, and relates to the technical field of hydrogel. The preparation method comprises the following steps: mixing a photo-thermal material with sodium alginate and sodium polyacrylate, adding lauryl sodium sulfate for foaming, and then performing freeze crosslinking and electrostatic self-assembly, thereby obtaining the photo-thermal composite material. The porous hydrogel based on the photo-thermal material prepared by the preparation method provided by the invention can efficiently realize interface evaporation and has excellent salt resistance and antibacterial performance, the pure water evaporation rate can reach 3.03 kg.m <-2 >. H <-1 >, the evaporation rate in 20% saline water is kept at 2.43 kg.m <-2 >. H <-1 >, and after the porous hydrogel is in dynamic contact with escherichia coli for 18 hours, macroscopic bacterial colonies do not exist, and the antibacterial rate reaches 99%; in addition, self-floating can be achieved, cost can be saved in practical application, and a new thought is provided for the interface evaporator for sea water desalination.

The invention discloses a preparation method and application of breathable and highly stretchable hydrogel. The preparation method comprises the following steps: firstly, foaming a gelatin solution through a foaming pump, and cooling and fixing to form porous hydrogel; and then soaking in a composite solution containing salt of a specific type and concentration to obtain the breathable and highly stretchable hydrogel. The method not only can simply, conveniently and efficiently construct the porous hydrogel with controllable porosity and aperture, but also overcomes the contradiction between the porous structure and high ductility. The obtained hydrogel has excellent air permeability, ductility, electrical conductivity and light weight, and can detect human motion information and electrophysiological signals by being applied to different epidermis positions of a human body. The invention provides a new thought and method for preparing a breathable and comfortable hydrogel flexible electrode, and is beneficial to the development of hydrogel electronic skin.

The present invention is in the technical field of additive manufacturing and provides a continuous method for producing planar, porous hydrogels and structures. The present invention also provides an apparatus for the continuous production of hydrogels.

The invention provides macroporous structure eutectic hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel preparation. The preparation method comprises the following steps: firstly preparing an MXene coated MWCNTs-NH2 dispersion liquid, then adding PVA and a pore-forming agent into the MXene coated MWCNTs-NH2 dispersion liquid to obtain a mixed liquid, then grinding the mixed liquid to obtain a pre-gel liquid, then carrying out circulating freezing-unfreezing on the pre-gel liquid, then soaking in an acidic solution to obtain a macroporous hydrogel, and finally, carrying out ultrasonic treatment on the macroporous hydrogel to obtain the porous hydrogel. Soaking the macroporous hydrogel in a mixed solution of a deep eutectic solvent and deionized water for solvent replacement, and obtaining the macroporous hydrogel after soaking; the prepared macroporous structure eutectic hydrogel has excellent mechanical property, conductivity, antibacterial property and environmental stability; the method can be applied to preparation of wearable sensors or intelligent artificial skins, and the prepared sensors have excellent sensitivity.

The invention provides a process for preparing a plasticised superporous hydrogel material comprising subjecting initial hydrogel material to treatment with an acidic solution, an optional treatment with a monovalent metal salt solution, freeze drying and plasticisation. Optionally, the process of the invention produces moulded plasticised superporous hydrogel material bodies that have one or more through-holes formed therein. The plasticised superporous hydrogel material of the present invention may be formulated as a suitable oral dosage form for use an appetite suppressant and for use to deliver a pharmaceutical and / or nutraceutical into a human or animal body.

The present application relates to the technical field of biological tissue engineering, and particularly relates to an artificial blood vessel and a preparation method thereof. The present application creatively develops an artificial blood vessel with a double-network interpenetrating structure based on a design of space-time coupling, wherein a double-layered openwork scaffold serves as a space maintenance network, and a porous hydrogel tube wall serves as a temporary filling network, so that accurate space-time adaptation of degradation and regeneration is realized. Specifically, the double-layered openwork scaffold serves as a main load-bearing framework, and the slow degradation characteristic of the double-layered openwork scaffold provides independent, stable and long-term mechanical support, thereby fundamentally avoiding premature degradation of mechanical properties. Meanwhile, the fast-degrading hydrogel tube wall ensures the airtightness of the blood vessel in the early stage after implantation, and then degrades rapidly to leave a preset and through three-dimensional space for tissue regeneration, thereby ensuring the speed and integrity of the regeneration.

Described herein are systems, methods, and interconnecting porous hydrogel blocks for ex vivo kidney tissue generation. A block may comprise a 3D continuous polymeric matrix with a network of microporous cavities, and may be configured to interconnect with at least one other block. A system may comprise at least one block and be configured to cultivate one or more kidney-like organ components when seeded with at least one HEK293 cell and at least one kidney cell isolated from at least one specimen. A method may comprise seeding at least one block with at least one HEK293 cell and at least one kidney cell isolated from at least one specimen

According to the graded photo-thermal-gradient needle tip array type saline-alkali soil evaporator and the preparation method thereof, cork wood-derived cellulose is used as a matrix raw material, a graded photo-thermal layer of carbon nano tubes and biochar is constructed, a gradient-size needle tip array is designed, and a porous hydrogel matrix structure is optimized, so that the graded photo-thermal-gradient needle tip array type saline-alkali soil evaporator is obtained. And the evaporator with the functions of efficient photo-thermal conversion, directional water transmission and thorough salt falling is prepared. The full-spectrum absorption efficiency of the prepared evaporator is larger than or equal to 95%, the salt crystallization falling rate is larger than or equal to 98%, the evaporation rate reaches 1.8 kg / mh under one sunlight intensity, the performance degradation is smaller than or equal to 5% after 100-hour continuous operation, the problems that a traditional evaporator is low in photo-thermal efficiency, salt is left and accumulated, and the water transmission resistance is large can be effectively solved, and the evaporator is suitable for saline-alkali soil treatment, fresh water generation and mineral salt collection scenes.

Described herein are systems, methods, and interconnecting porous hydrogel blocks for cell culture. A block may comprise a 3D continuous polymeric matrix with a network of microporous cavities, and may be configured to interconnect with at least one other block. A system may comprise at least one block and be configured to be perfused via at least one cavity by at least one perfusate under one or more controllable flow rates, pressures, volumes, viscosities, and / or osmotic pressures. A method may comprise exposing at least one block to at least one perfusate under one or more controllable flow rates, pressures, volumes, viscosities, and / or osmotic pressures.

Embodiments relate to porous hydrogel microparticles, porous granular hydrogel scaffolds, and methods of making and using thereof. A method of making porous hydrogel microparticles includes crosslinking first polymers and second polymers to form composite microgels, adding the composite microgels to a liquid solution at a first temperature to form a composite microgel suspension, reducing the temperature of the composite microgel suspension to a second temperature below a phase separation temperature such that the second polymers separate from the first polymers, and filtering the composite microgel suspension from the liquid solution such that the second polymers diffuse out of the composite microgel suspension, resulting in the porous microgels.

The invention discloses an expandable self-adaptive bionic tissue repair nail. The expandable self-adaptive bionic tissue repair nail comprises a nail cap and a nail body which are made of biodegradable materials, the nail cap is a double-layer film layer, and the layer, away from the nail body, of the nail cap is a smooth compact layer. The layer, close to the nail body, of the nail cap is a porous support layer, and the surface of the porous support layer is integrated with a combining mechanism tightly attached to the tissue surface. The nail body is of a hollow net-shaped structure, and the inner cavity of the nail body is filled with swellable and biodegradable porous hydrogel; the nail body can be in a compressed and folded compact state in a dehydration state and is expanded and unfolded after encountering water; drugs are loaded in the porous hydrogel. Before implantation, the repair nail is in a dehydrated slender state; after implantation, the internal hydrogel swells when encountering liquid, an irregular fistula channel is filled with the net-shaped structure in a self-adaptive mode, meanwhile, an inner opening is sealed by the nail cap, physical bacteria blocking is achieved, and the nail cap serves as a regeneration support to actively promote repair. The device can be implanted in a minimally invasive mode, can expand in a self-adaptive mode after operation so as to be tightly attached to tissue, and meanwhile has the anti-infection function and the active repair promoting function.

The invention discloses an application of gelatinized intestinal epithelial cells in preparation of drugs for treating inflammatory bowel disease, the gelatinized intestinal epithelial cells take mouse intestinal epithelial cells as cores, poly (ethylene glycol) diacrylate, a photoinitiator and poly-L-lysine permeate into the cells through low-temperature freeze thawing, and an intracellular porous hydrogel skeleton is formed through ultraviolet crosslinking. The gelated intestinal epithelial cell has a strong cfDNA and inflammatory factor removal function, has good biocompatibility, has no direct or indirect toxic effect on a human body, has no potential toxicity, and can be used for treating inflammatory bowel diseases.

The present invention belongs to the technical field of preparation of functional materials for adsorption and separation, and discloses a method for preparing underwater adhesive porous hydrogel uranium extraction adsorbent based on a liquid-gas droplet reactor. Using amidoxime functional groups as selective ligands, and poly(N-isopropylacrylamide-co-acrylic acid) microgel P(NIPAM-co-AAc)-HEMA functionalized with hydroxyethyl methacrylate (HEMA) as a polymer matrix and surfactant, an amidoxime-functionalized porous hydrogel-based adsorbent PAOmgel with underwater adhesion properties was prepared by treatment with a dopamine solution. The present invention achieves efficient and selective extraction of uranyl ions by introducing polyamidooxime functional units. The use of a Pickering liquid-gas droplet reactor provides a clean and effective method for the controllable preparation of porous structures, utilizes the water absorption and porosity of the hydrogel to accelerate the mass transfer kinetics of the adsorbent, and the underwater adhesion characteristics simplify the adsorbent recovery process, solving the problem of difficult recovery of uranium extraction adsorbents.

This invention discloses a method for preparing a colorimetric sensing gel for formaldehyde detection, and the corresponding colorimetric sensing gel. The preparation method includes the following steps: (1) grafting aldehyde groups; (2) grafting sensing molecules; (3) preparation of porous gel and core-shell structure. This invention uses chemical doping to graft amino-rich polyamine sensing molecules onto green and renewable calcium alginate to prepare a low-cost, green porous hydrogel for detecting formaldehyde concentration in the air.

The invention discloses a preparation method and application of a flexible supercapacitor electrode material. A porous hydrogel matrix with a semi-interpenetrating network structure is constructed by using carboxymethyl nanocellulose and sodium alginate, and a conductive polymer polypyrrole PPy is assembled by using a one-step oxidation in-situ polymerization reaction, so that a hydrogel supercapacitor electrode material with high conductivity and high area specific capacitance is successfully prepared. The method is simple to operate and low in cost, and the used raw materials are green and environment-friendly. The electrode material has good structural stability and excellent electrochemical performance, and can be directly stacked in combination with a carbon cloth current collector and filter paper without a cross-linking agent after being soaked in an electrolyte to be applied to a high-performance all-solid-state supercapacitor.

The invention provides a bilayer active biological aerosol sampling membrane of a bionic peritoneum and a preparation method of the bilayer active biological aerosol sampling membrane of the bionic peritoneum, the sampling membrane is formed by compounding a functional capture layer and an activity maintaining base layer through interface crosslinking, and the functional capture layer is composed of a hydrophilic modified polypropylene porous film. The activity maintaining base layer is composed of porous hydrogel loaded with nutrient substances. The preparation method comprises the following steps: preparing the functional capture layer; preparing an activity maintaining base layer; and compounding. Through the bionic peritoneum structural design, the bioaerosol can be efficiently intercepted, and meanwhile, the activity of microorganisms in the bioaerosol can be maintained for a long time.

The invention belongs to the field of biomedical engineering materials, and particularly relates to injectable 3D porous composite hydrogel with a BMP-2 slow release function as well as a preparation method and application of the injectable 3D porous composite hydrogel. According to the present invention, the porous hyaluronic acid methacrylate hydrogel is developed, and the porous hyaluronic acid methacrylate hydrogel is used for wrapping the adipose-derived stem cells (ADSCs) and the bone morphogenetic protein-2 (BMP-2), such that the porous hyaluronic acid methacrylate hydrogel can be used for preparing the bone morphogenetic protein-2, and the porous hyaluronic acid methacrylate hydrogel can be used for preparing the bone morphogenetic protein-2, and can be used for preparing the bone morphogenetic protein-2, the bone morphogenetic protein-2, the bone morphogenetic protein-2, the bone morphogenetic protein-2, the bone morphogenetic protein-2 and the bone morphogenetic protein-2. In-vitro experiments prove that the ADSCs have remarkable proliferation and directional migration characteristics in the porous hydrogel system. After the ADSCs-loaded composite hydrogel (HMs / MBs / ADSCs) is applied to a rat rotator cuff injury model, a fibrous cartilage transition zone is effectively reconstructed, and the healing of a tenon-bone interface (tenon-bone interface, TBI) is promoted by the ADSCs-loaded composite hydrogel (HMs / MBs / ADSCs). In-vitro sequencing is further combined to find that HMs / MBs / ADSCs regulate and control the paracrine effect of ADSCs through BMP-2, so that the tissue microenvironment is improved to enhance the regeneration efficiency. According to the system disclosed by the invention, a comprehensive pathway of HMs / MBs / ADSCs for promoting tissue regeneration is clarified, a synergistic effect mechanism of BMP-2 and ADSCs is disclosed, and a new insight is provided for a biological mechanism of TBI healing.