1598results about How to "Convenient for clinical operation" patented technology

The present invention discloses novel dendritic cell maturation-inducing cytokine cocktails, and methods for inducting type-1 polarized dendritic cells in serum-free conditions which enhance the desirable properties of DC1s generated in serum-supplemented cultures. The invention further discloses methods and systems using IFNγ and other ligands of the IFNγ receptor, in combination with IFNα (or other type I interferons), poly I:C, and other IFNα (and IFNβ) inducers to enhance the IL-12-producing properties of dendritic cells. More specifically, the present invention discloses type-1 polarized dendritic cells that have a unique combination of a fully-mature status and an elevated, instead of “exhausted”, ability to produce IL-12p70. allows for the generation of fully-mature DC1s in serum-free AIM-V medium. The invention discloses systems that use the foregoing products and methods to facilitate the clinical application of DC1-based vaccines and the identification of novel factors involved in the induction of Th1 and CTL responses by DC1.

The invention relates to a method for preparing a biomaterial of acellular small intestinal submucosa, which comprises the steps of preposition treatment, acellular treatment, enzyme treatment, preparations of membranous products and particle products. Compared with the prior art, the biomaterial has higher bioactivity and biocompatibility, no obvious immune rejection and no toxic effect on cells; besides, the biomaterial has a certain mechanical strength and toughness, and variable shape, size and thickness, thereby being convenient for clinical suturing and fixing. At the same time, the preparation method has the advantages of unlimited raw material source, cell-free residues, no ethical issues and being capable of effectively inactivating virus. The prepared products are applicable to the biomedical engineering fields, such as repairing defections of body tissue, serving as tissue filling materials, repairing facial depression deformity, serving as tissue reinforcements to replace fascia, repairing membranous defections and malnourished and infected surfaces of wound, serving as materials for biodegradable stents, and serving as injectable filling materials.

The invention provides a nano artificial dura mater with a plurality of functional layers, which comprises a structure with at least two layers; the layer facing the brain is a hydrophobic anti-adhesion electrospun layer; and the layer back to the brain is a hydrophilic nano cytoskeletal layer. The invention also provides a method for preparing the nano artificial dura mater, which comprises the following steps: preparing an electrospun solution of a hydrophobic polymer; preparing an electrospun solution of a hydrophilic polymer; preparing the prepared electrospun solution of the hydrophobic polymer into the hydrophobic anti-adhesion electrospun layer through static spinning; and preparing the hydrophilic nano cytoskeletal layer on the anti-adhesion electrospun layer from the prepared electrospun solution of the hydrophilic polymer through static spinning, receiving a membrane structure, and obtaining the nano artificial dura mater. The mechanical features of the nano artificial dura mater can meet the requirements on tensile strength and flexibility of adaptation diseases; and the nano artificial dura mater can be freely cut as required, and has wide application prospect.

The present invention is objective, quantitative and precise method of predicting serious complication risk degree after gastric cancer operation. The method includes the following steps: 1. screening out factors affecting the serious complication after gastric cancer operation through overall single factor analysis; 2. determining the prognostic determining factor through two-value multiple non-conditional logic regression analysis; 3. determining the optimal prediction value dividing value through the work characteristic analysis on the testee; and 4. establishing the predicting model with main risk factor as the independent variable and judging the serious complication risk degree after gastric cancer operation by means of the prediction probability. The present invention is one objective and quantitative intelligent risk evaluation system.

The invention provides a medical anti-sticking membrane and a preparation method thereof. The anti-sticking membrane comprises a nanometer frame and hydrosol attached thereon. The hydrosol is internally packed with one or several kinds of styptic medicament or / and anti-sticking medicament. The invention also provides a preparation method of the anti-sticking membrane, comprising the following steps of: preparing electro-spinning solution, styptic medicament and / or anti-sticking medicament-containing hydrosol solution and crosslinker solution; receiving static spinning with the crosslinker solution to obtain the nanometer frame; printing the styptic medicament and / or anti-sticking medicament-containing hydrosol solution onto the nanometer frame by an ink-jet printer, and solidifying the hydrosol solution to obtain the anti-sticking membrane. The anti-sticking membrane has good capability and biological compatibility, and nontoxicity as well as nonirritant, can be completely degraded and absorbed, is compounded with controllably released styptic medicament or / and anti-sticking medicament, has controllable degrading time and speed, and conquers defects of the prior art.

A non-contact type dental shade matching device is provided, comprising a camera body for capturing image of one or more target teeth; an opaque intra-oral compartment snugly adapted for a human mouth; an opaque cover shield body connected between the camera body and the intra-oral compartment; one or more holders for holding one or more shade tabs; and a color matching module being operably connected to the camera body to receive the captured images containing color and translucency information of the target tooth and / or shade tab information, and then to process the images based on a content-based algorithm for automatic shade matching between the target tooth and the shade tabs for each of the captured images, so as to achieve an optimal dental prosthesis.

The invention refers to a preparation method of ginseng polysaccharide. The method includes the following step: extract it from the ginseng or the marc of the ginseng decocted, filtrate, concentrate and deposit the extracting solution, get the precipitate after centrifugence and dry it with low temperature to get the crude product of ginseng polysaccharide. We get the polysaccharide product after the ginseng polysaccharide goes through the boiling, depositing, filtration, trash extraction, frost thawing, getting rid of the protein and getting the supernatant and the supernatant passes through the concentration with low pressure and drying with low temperature. We can make it into all kinds of forms of prepared drugs after the ginseng polysaccharide passes through the experiments in pharmaceutics. The invention effectively increases the quality of the ginseng polysaccharide injection, fully exerts the immunoloregulation function of the ginseng polysaccharide, increases the anti tumor effect of chemotherapy medicament and is used to reduce the side effect caused by the knubbly radiotherapy and chemotherapy as well as adjuvant for curing the tumor.

The invention relates to the technical field of medical dynamic measurement analysis, in particular to a wearable sensor measuring device and method for lower limb joint acting force and moment. The device consists of a computer memory, a data collector and wireless Bluetooth communication module of software and the like. The device is characterized in that a pelvis is provided with a first sensor, and a left huckle and a right huckle are provided with a second sensor and a third sensor; a lower left leg and a lower right leg are provided with a fourth sensor and a fifth sensor, and the side parts of a left heel and a right heel are provided with a sixth sensor and a seventh sensor; the side part of a right forefoot is provided with a eighth sensor and a ninth sensor, and a tenth sensor, an eleventh sensor, a twelfth sensor and a thirteenth sensor are arranged at the upper position and the lower position of a left planta and a right planta; and sensor data test signals are input into the signal input end of a computer to be collected and operated. The device can furthest reduce the influence of estimation errors of the quality, the center of mass and the moment of inertia of each part of lower limbs on the joint force and moment calculation accuracy. According to the method, the calculated amount can be reduced, and the application to real-time measurement and control is favorably realized.

The invention discloses an acellular matrix with natural level of glycosaminoglycan and preparation and application thereof. The preparation method comprises the following steps: carrying out acellular disposal on natural biological tissue to obtain an acellular matrix; detecting missing amount of glycosaminoglycan in the acellular matrix; and supplementing glycosaminoglycan by means of natural ingredients in the acellular matrix to obtain the acellular matrix with natural level of glycosaminoglycan. Through the method, a lot of the glycosaminoglycan component in the acellular matrix is recovered. The acellular matrix has good biocompatibility. The natural biological structure is recovered, and the acellular matrix is beneficial to cell growth. glycosaminoglycan is supplemented for the acellular matrix, and a tissue engineering carrier is constructed. According to the invention, a biological carrier which meets cell growth requirement and mechanical strength requirement and has similar structural components as a natural extracellular matrix can be prepared rapidly. The invention is a great breakthrough of the tissue engineering construction technology. Meanwhile, principle of the invention is reliable, the technology is simple and flexible, product reproducibility is good, and industrialization is easy to realize.

A high-stability freeze-dried powder injection of omeprazole sodium is proportionally prepared from omeprazole sodium, Na2EDTA and mannitol. Its preparing process is also disclosed.

The invention belongs to the field of maxillofacial plastic medical instruments, and in particular relates to a CT (computerized tomography) and CBCT (cone beam computerized tomography) fusion data based digital occlusion guide plate and a reconstruction method thereof. The guide plate has a horseshoe-shaped body, and the guide plate body is provided with a guide plate upper jaw surface, a guide plate lower jaw surface, a guide plate lip outer side and a guide plate lip inner side, wherein the guide plate upper jaw surface and the guide plate lower jaw surface are provided with a groove respectively, and the shape of each groove is fit with the shape of a corresponding tooth crown. Aiming at the difficulty of reconstructing an occluding relation and the problems that the surgical precision of a conventional model needs to be improved and the like, a CBCT and spiral CT fusion data based virtual occlusion reconstruction method, by simulating operative reduction, virtually reconstructing the occluding relation, designing an occluding plate, recording the occluding relation and performing 3D printing to generate a guide plate real object, is used for operatively reconstructing the occluding relation, reducing the operation difficulty, shortening the operation time, ensuring the operation effect, and improving the reconstruction precision.

The invention belongs to preparation of biological cell formulations, and in particular relates to a preparation method of dendritic cell (DC) vaccine loaded with autologous tumor associated holoantigen. The preparation method comprises the following steps: preparing the autologous tumor associated holoantigen, collecting and separately culturing DCs, impacting the DCs by the autologous tumor associated holoantigen, maturing the DCs and preparing autologous tumor antigen specific DC vaccine. The invention solves the problems in the prior art that the immunogenicity of tumor antigen is not strong enough, the antigen target spots are incomplete, the tumor antigens of most tumor patients are difficult to acquire, and the like. The DC vaccine provided by the invention has the advantages of effectively inducing tumor antigen specific cytotoxic T lymphocyte (CTL) in vitro and in vivo, efficiently generating specific cytotoxicity on tumors, having high overall effective rate and no obvious toxic side effects in clinical application, and the like.

The invention discloses a Lansoprazole preparation for injection comprising (1) Lansoprazole 10-50 weigh parts, (2) meglumine 2-20 weight parts, (3) sodium hydroxide 1-5 weight parts, (4) mannitol 20-100 weight parts. The invention also discloses the process for preparing the preparation.

The invention provides a medical dressing for protecting skin wounds and promoting repairing, an application of the medical dressing and a preparation method of the medical dressing. The medical dressing comprises a biological plural gel film dressing and a medical polyurethane adhesive film; the biological plural gel film dressing is composed of raw materials of a framework material synthesized by polyanion substances and polycation substances, hyaluronic acid, polypeptide and bonding polypeptide; and the potential of hydrogen (pH) value of the biological plural gel film dressing is 5.8-6.4. The medical dressing for protecting the skin wounds and promoting repairing of the skin wounds has the advantages of wound covering medical dressings, more importantly, by means of synergistic actions of the bonding polypeptide and the hyaluronic acid, the structural designs of medical dressing materials are optimized, and additional growth factors or living cells are not required for enabling the medical dressing materials to have functions of in-situ inducing tissue regeneration and repair.

An improved encephalic puncture guiding method comprises the following steps of 1, puncture planning guidance, wherein 1.1, an encephalic scanning image sequence is loaded, a three-dimensional encephalic model is built, and meanwhile measurement angle correction data are loaded; 1.2, reference points are set, perpendicular reference planes are built in the three-dimensional model; 1.3, a target spot and an entry point are set, the length of a puncture path composed of the target spot and the entry point and the included angle between the puncture path and the reference planes are worked out; the final plan puncturing angle and depth are obtained by correcting the angle correction data; 2, puncture execution, wherein in the step 1.3, when an encephalic coordination system and a ground coordinate system are not consistent, the encephalic coordination system and the ground coordination system are registered by adopting the point registration method based on output data of a gyroscope, and the included angle between the reference planes and the puncture path obtained after registration is automatically worked out. The invention provides an improved encephalic puncture guiding device. The improved encephalic puncture guiding method and device are easy to implement and convenient to operate and facilitate clinical application.

The invention discloses a multichannel synchronous real-time digitalized photoacoustic imaging device and method. The multichannel synchronous real-time digitalized photoacoustic imaging device comprises a laser, a multi-element ultrasonic detector, a front amplification circuit and a data imaging processing unit, wherein the data imaging processing unit comprises a plurality of field programmable gate array (FPGA) acquisition cards, a clock synchronous board card, a programmable communication interface (PCI) extensions for instrumentation express (PXIe) backboard and a central processing unit (CPU) board card, and can be used for realizing acquisition processing for at least 32 paths of synchronous photoacoustic signals. The multichannel synchronous real-time digitalized photoacoustic imaging method comprises the following steps that: the multi-element ultrasonic detector receives the photoacoustic signals produced by laser irradiation on a biological tissue, amplifies the photoacoustic signals and respectively transmits the signals to the FPGA acquisition cards; and each FPGA acquisition card performs preprocessing, deconvolution, continuous wavelet transformation and photoacoustic attenuation compensation on the signals, then transmits the signals to another FPGA acquisition card in a peer-to-peer (P2P) manner, performs imaging processing by adopting a 2-D (two-dimensional) filtration back-projection algorithm, and finally transmits the structure to an upper computer. The method is finished on an FPGA processor; according to a modular design, the expansion of the large-scale detector is simplified; and the device and the method are favorable for clinical application of photoacoustic imaging systems.

The invention provides a nano artificial dura mater capable of being used as a medicine sustained-release system, having the structure which comprises at least two layers, i.e. a hydrophobic anti-blocking electro-spun layer which faces the cerebrum, and a hydrophilic nano cytoskeleton layer which backs on to the cerebrum; and cell factors and / or medicines are arranged in any layer of the artificial dura mater by way of blended spinning. The invention also provides a method for preparing the nano biomimic artificial dura mater. Compared with an artificial mater prepared by the single utilization of the electro-spinning technology, the artificial mater to which the medicines and the cell factors are added by blending technology can effectively prevent infection and faster promote the regeneration process of the artificial mater. The invention also provides a novel medicine loading and releasing mode for treating cerebral diseases, the loaded medicines can be directly and efficiently transferred into the cranial cavity along with the implantation of the dura mate and can be released according to requirements, and therefore the invention realizes favorable treatment effect and has broad application prospect.

The invention discloses a direct venous re-transfusion immune cell cryopreservation medium and application thereof. The direct venous re-transfusion immune cell cryopreservation medium is prepared from dimethyl sulfoxide, human serum albumin, dextran, hydroxyethyl starch, heparin sodium and other clinical medicinal level raw materials by optimizing the proportion of the dose of each raw material. In addition, the operation steps are simplified and the cryopreservation effect is improved by optimizing the cryopreservation method. On one hand, the potential risk of introducing an animal source antigenic substance in the cell treatment process is avoided, and on the other hand, the cryopreservation effect of immune cells is better than that of a conventional cryopreservation medium, thereby guaranteeing the safety and validity in clinical use. Two kinds of components are used for preservation, so that the cryopreservation medium can be preserved stably for a long time. The method disclosed by the invention is simple in operation, provides stronger operability for implementation of large-scale cryopreservation, and simultaneously provides a fundamental technical guarantee for implementation of immune cell treatment.

The invention relates to a nano micelle of a biodegradable macromolecular-bonding Pt(IV) anti-cancer medicament and a preparation method thereof. The structural formula of the anti-cancer medicament is defined in the specification, wherein the biodegradable macromolecule is tri-block copolymer, namely polyethylene glycol-b-polyester-b-polylysine; and tetravalency platinum coordination compound Pt(IV) is connected with a side amino on the block of polylysine on a macromolecular carrier through alpha, omega-polyethylene glycol. The carrier is non-toxic and is water-soluble, thus being convenient for reacting with the platinum(IV) coordination compound in water phase; a nano micelle form can be formed through self assembly; a platinum(IV) coordination compound is reduced to platinum(II), namely, cis-platinum, Carboplatin or Oxaliplatin, and the anti-cancer effect is known; the synthesis of the nano micelle is easy; the reduction potential of platinum(IV) is low, so that the platinum(IV) can be rapidly reduced to platinum(II) in cancer cells to take treatment effect; and the platinum(IV) is connected to the side chain of the macromolecule rather than the end of a chain, a macromolecular chain can be connected with multiple platinum(IV)s, and the content of platinum can be as high as 10-20%.

The invention provides high-strength hydrogel based on a three-dimensional network support and a preparation method of the high-strength hydrogel. The high-strength hydrogel is formed by interfingering and in-situ polymerization of hydrogel and the three-dimensional network support. The method comprises the following steps: immersing the three-dimensional network support in a hydrogel prepolymer solution; enabling the hydrogel to penetrate in the three-dimensional network support after in-situ polymerization forming, and enabling an interpenetrating polymer network to be formed between the hydrogel and the support by polymerizing and winding of macromolecule long chains through support meshes. The high-strength hydrogel based on the three-dimensional network support has the advantages thatthe prepared high-strength hydrogel has excellent wear resistance and mechanical strength resisting various direction forces such as stretching, compression and shearing, and a severe biomechanical property requirement to a replaced body in a physiological environment of a using portion can be met; and the tribological property of the surface of a complex is regulated and controlled effectively,the interfacial friction coefficient is reduced, and simulation to a self-lubricating function of a hyaline cartilage of a joint is realized. The method has simple operation steps, and is low in energy consumption, the materials have good biological compatibility, and the high-strength hydrogel can be produced on the large scale.

A staltic soluble cellulose sponge is proportionally prepared from carboxymethyl cellulose sodium and chitosan through dissolving carboxymethyl cellulose sodium in distilled water, dissolving chitosan in diluted acetic acid, mixing, adding polyethanediol 400, cane sugar and glycerin, stirring, laying aside while defoaming, filming, and vacuum freeze-drying.

The invention discloses a lung cancer early diagnostic marker based on metabonomics and a screening method thereof. The diagnostic marker comprises any one or combination of various markers in 25 plasma metabolism markers. The invention also provides a method for establishing a diagnostic model through utilization of the lung cancer early diagnostic marker and application of the lung cancer earlydiagnostic marker in a diagnostic kit. According to the lung cancer early diagnostic marker, the methods and the application, through utilization of a high performance liquid chromatography-mass spectrometry, non-target metabonomics analysis is carried out on plasma of patients; through utilization of an artificial intelligence data analysis technology, different metabolite between lung cancer patients and normal people is discovered; and further through target metabonomics analysis and machine learning modeling, diagnostic capability of specificity different metabolite, namely, the lung cancer early diagnostic marker in lung cancer early diagnosis is verified.

The invention discloses a quercetin nano-micelle preparation which is made from the following components in parts by weight: 20-40 parts of quercetin as a bulk drug and 220-330 parts of a pluronic surfactant as a drug carrier. A preparation method comprises the following steps: mixing the quercetin with the pluronic surfactant and dissolving in an organic solvent until being fully dissolved, and carrying out rotary evaporation at 15-60 DEG C for removing the organic solvent to obtain a drug-containing film; and drying the film in vacuum, adding a water phase for hydrating at 15-60 DEG C to obtain micelle solution, and filtering with a millipore filter to obtain the quercetin nano-micelle preparation. The obtained nano-micelle preparation has small nano-grade particle size of 20-60nm, a hydrophobic core and a hydrophilic shell, thus ensuring that the preparation has small possibility of being swallowed by a reticuloendothelial system in body, achieving effects such as targeting, long circulating and the like, and being beneficial to clinical application.

The three dimensional measuring device used in the dental field and aimed at measuring in the absence of projection of active or structured light, includes an image-capturing device and data-processor for images. The image-capturing device is capable of simultaneously, or almost simultaneously, capturing at least two images, one of which is totally or partially included in the other one. The included image describes a narrower field than that of the other one, and has a higher accuracy than that of the other one.

A freeze-dried liposoluble vitamin with high water solubility and stability and its preparing process is also disclosed.

The invention relates to a dispersion system with docetaxel as main component for injection, comprising a surfactant, an assistant surfactant and a specified amount of oil for injection. The volume ratio of oil phase to the surfactant is greater than 3 percent as well as less than and equal to 33 percent. The volume ratio of the assistant surfactant to surfactant is greater than or equal to 12.5 percent and less than 167 percent.

The invention relates to the technical field of gene detection, in particular to a CRISPR (Clustered regularly interspaced short palindromic repeats) / Cas12 one-step nucleic acid detection method and a2019-nCoV detection kit. The 2019-nCoV detection kit comprises crRNA, Cas protein, a primer, a buffer system and single stranded DNA reporter molecules; the primer is obtained as follows: 5'-TTTN-3'sequence is sought in a to-be-detected target molecule area, a forward primer is designed in the position near 5' area 0-200bp, a reverse primer is designed in the position near 3' area 25-200bp, theobtained primer is subjected to chemical modification to prevent decomposition of DNA enzyme or Cas12 protein after activation. The method has the benefits that compared with existing PCR-based nucleic acid detection technologies, the nucleic acid detection method and the 2019-nCoV detection kit can allow synchronous amplification and detection, thus, operation is more convenient.

The invention discloses a paclitaxel alhumin submicron for injection. The paclitaxel alhumin submicron comprises 1-10% (W / W) of paclitaxel, 10-80%(W / W) of alhumin and 10-85% (W / W) of freeze-drying protective agent. The invention also discloses a preparation method of the paclitaxel alhumin submicron. The method comprises the following steps of 1) adding paclitaxel into tert-butyl alcohol, stirring and dissolving to be used as an organic phase; 2) adding the alhumin and the freeze-drying protective agent to water or a buffer with a pH within 6.0-8.5, stirring and dissolving to be used as a water phase; 3) adding the organic phase into water with stirring, stirring and dissolving, filtering and degerming through a millipore filtration method, and placing in a clean container; 4) freezing at-30- -50 DEG C, removing tert-butyl alcohol and water through freeze-drying by a lyophilizer to obtain freeze-drying powder with good profile and loosening quality. During usage, the freeze-drying powder is added to a solvent for injection and redissolved, so that paclitaxel alhumin submicrons with an average particle size of 100-600nm are formed. The paclitaxel alhumin submicron for injection ofthe invention has advantages of a simple preparation technology, no high toxic organic solvent and long-term storage, etc.

The invention discloses a measuring method and system for single-side osteal damage of the human body. The corresponding osteal form at the uninjured side serves as a template, a 3D osteal CT image of the uninjured side is overturned to form a mirror image, and a 3D osteal CT image of the injured side is subtracted from the mirror image to obtain the accurate form of osteal defect at the injured side; and the parameters including the maximal length, width, depth and spatial position of the osteal defect position are measured to make comprehensive evaluation for the osteal defect of the injured side. Model reconstruction and 3D measurement are realized for single-side osteal defect of human body, the osteal defect can be evaluated accurately and comprehensively, and the method and system have great significance in effectively reducing the post-operation recurrence rate caused by the osteal defect.

The invention relates to a multifunctional particle used for stopping human body wounds bleeding, filling, absorbing fluid, preventing adhesion and inducing tissue regeneration and repair. The absorbable particle with multiple functions of stopping bleeding, filling, absorbing the fluid, preventing the adhesion and inducing the tissue regeneration and repair comprises the following raw materials in weight percentage: 30-98.95 percent of polyanionic substance, 1-70 percent of cationic substance and / or polycationic substance, 0.05-2 percent of hyaluronic acid and / or functional factor polypeptide and 0-68.95 percent of pharmaceutical excipient. The particle is 0.01-1.0mm in particle size, and the particle is irregular in shape. The absorbable particle material with the multiple functions of stopping bleeding, filling, absorbing the fluid, preventing the adhesion and inducing the tissue regeneration and repair can be used for stopping the human body wounds bleeding, filling, absorbing fluid, preventing the adhesion and inducing the tissue regeneration and repair and is especially suitable for the wounds and defective tissues during minimally invasive surgery.