The invention discloses a preparing method for pig
thrombin freeze-dried
powder. The preparing method comprises the following steps that
anticoagulant pig
plasma and gel are mixed and stirred for adsorption and pass through a column for
elution, and a prothrombin solution is obtained; normal
saline is added to
rabbit brain powder, then the prothrombin solution and CaCl2 are added for
zymogen activation, a crude
thrombin enzyme solution is obtained, subjected to
ultrafiltration for
desalination and concentrated,
viral inactivation is carried out, an obtained crude
enzyme solution passes through DEAE-
Sepharose Fast Flow chromatographic column,
elution is carried out, target peaks are collected, and pig
thrombin is obtained; the pig thrombin is added with
mannitol or
dextran 40, filtering, sterilizing, subpackaging,
freeze drying and vacuum tamponing are carried out, an aluminum plastic combined cover is rolled, and a pig thrombin freeze-dried product is obtained; the freeze-dried product is subjected to
dry heat treatment at 100 DEG C for secondary
virus inactivation, and the pig thrombin freeze-dried
powder is obtained after packaging. The
specific activity of pig thrombin in the product is not lower than 130 U / mg, the whole process is simple in step and easy to implement, the product safety is improved, and the preparing method is suitable for industrial production.