Microfiber collagen hemostatic material and preparation method thereof

Abstract

The invention relates to a microfiber collagen hemostatic material and a preparation method thereof. The preparation method of the material includes the first step of sodium sulfide unhairing, the second step of shredding and fibrosis, the third step of swelling control, the fourth step of drying, the fifth step of depolymerisation and fluffing, and the sixth step of Co60 radiation sterilization, wherein in the fifth step, the obtained fasciculus is treated using a hammer mill to obtain a microfiber collagen product. According to the method, the use concentration of sodium sulphide is reduced, the efficiency of unhairing is improved, and degreasing and impure protein removal are effectively conducted; collagen materials are not oxidized and degenerated, the activity of microfiber collagen is easy to maintain, and the hemostatic effect is improved. Acid is introduced in the treating process, the degreasing and protein removal are more effective, and the adhesion property of the hemostatic material is guaranteed.

Description

technical field

[0001] The invention relates to the field of biopharmaceuticals, in particular, the invention relates to a hemostatic material and a preparation method thereof. Background technique

[0002] The statistical results of clinical practice show that injury or uncontrollable postoperative bleeding is the main cause of death. Hemostasis means are very important in the medical field. Before hemostasis and complete hemostasis, it is very important to shorten the hemostasis time and reduce the amount of blood loss. If the bleeding cannot be completely stopped, the bleeding during the operation will affect the vision and increase the difficulty of the operation, and the bleeding after the operation will lead to infection, which will lead to difficulties in post-operative care and even reoperation. In recent years, medical absorbable hemostatic materials have attracted great attention from the medical and industrial circles of various countries. Many large pharmaceutica...

Examples

Embodiment 1

[0072] 1. Depilate cowhide with a depilatory agent containing 4wt% sodium sulfide and 40vol% ethanol.

[0073]2. Cut raw bovine leather into 3.0cm squares and freeze for later use.

[0074] 3. Thaw for 1 day, wash with 2-8°C aqueous solution for several times, and after removing excess water, use a cutting machine to cut the cowhide into small pieces or pieces of 0.60-1.50cm.

[0075] 4. The cut pieces are mixed with crushed ice and then fiberized by a cutting machine, so that the solid content after fiberization is 35 wt%, and centrifuged to make the solid content 40 wt%.

[0076] 5. All liquid handling is carried out between 25-28°C. To 12.5 kg of the fibrillation mixture was added a mixture of 37.5 kg of ethanol and 200 mL of 12N hydrochloric acid (concentrated hydrochloric acid). Mix and stir for 5 hours, and centrifuge to make the solid content 50wt%.

[0077] 6. Add 31.7kg of ethanol and 317g of Tween 80 mixture to the above centrifuged mixture, mix and stir for 3h, a...

Embodiment 2

[0083] 1. adopt the depilatory agent that contains 4wt% sodium sulfide, 40vol% ethanol to depilate cowhide,

[0084] 2. Cut raw bovine leather into 3.0cm squares and freeze for later use.

[0085] 3. Thaw for 1 day, wash with 2-8°C aqueous solution for several times, and after removing excess water, use a cutting machine to cut the cowhide into small pieces or pieces of 0.60-1.50cm.

[0086] 4. The cut pieces are mixed with crushed ice and then fiberized by a cutting machine, so that the solid content after fiberization is 35%, and centrifuged to make the solid content 40wt%.

[0087] 5. All liquid handling is carried out between 25-28°C. To 12.5 kg of the fibrillation mixture was added a mixture of 37.5 kg of ethanol and 200 mL of 12N hydrochloric acid (concentrated hydrochloric acid). Mix and stir for 5 hours, and centrifuge to make the solid content 50wt%.

[0088] 6. Add 31.7 kg of ethanol and 317 g of Tween 80 mixture to the above centrifuged mixture, mix and stir for ...

technical field

[0001] The invention relates to the field of biopharmaceuticals, in particular, the invention relates to a hemostatic material and a preparation method thereof. Background technique

[0002] The statistical results of clinical practice show that injury or uncontrollable postoperative bleeding is the main cause of death. Hemostasis means are very important in the medical field. Before hemostasis and complete hemostasis, it is very important to shorten the hemostasis time and reduce the amount of blood loss. If the bleeding cannot be completely stopped, the bleeding during the operation will affect the vision and increase the difficulty of the operation, and the bleeding after the operation will lead to infection, which will lead to difficulties in post-operative care and even reoperation. In recent years, medical absorbable hemostatic materials have attracted great attention from the medical and industrial circles of various countries. Many large pharmaceutica...

Examples

Embodiment 1

[0072] 1. Depilate cowhide with a depilatory agent containing 4wt% sodium sulfide and 40vol% ethanol.

[0073]2. Cut raw bovine leather into 3.0cm squares and freeze for later use.

[0074] 3. Thaw for 1 day, wash with 2-8°C aqueous solution for several times, and after removing excess water, use a cutting machine to cut the cowhide into small pieces or pieces of 0.60-1.50cm.

[0075] 4. The cut pieces are mixed with crushed ice and then fiberized by a cutting machine, so that the solid content after fiberization is 35 wt%, and centrifuged to make the solid content 40 wt%.

[0076] 5. All liquid handling is carried out between 25-28°C. To 12.5 kg of the fibrillation mixture was added a mixture of 37.5 kg of ethanol and 200 mL of 12N hydrochloric acid (concentrated hydrochloric acid). Mix and stir for 5 hours, and centrifuge to make the solid content 50wt%.

[0077] 6. Add 31.7kg of ethanol and 317g of Tween 80 mixture to the above centrifuged mixture, mix and stir for 3h, a...

Embodiment 2

[0083] 1. adopt the depilatory agent that contains 4wt% sodium sulfide, 40vol% ethanol to depilate cowhide,

[0084] 2. Cut raw bovine leather into 3.0cm squares and freeze for later use.

[0085] 3. Thaw for 1 day, wash with 2-8°C aqueous solution for several times, and after removing excess water, use a cutting machine to cut the cowhide into small pieces or pieces of 0.60-1.50cm.

[0086] 4. The cut pieces are mixed with crushed ice and then fiberized by a cutting machine, so that the solid content after fiberization is 35%, and centrifuged to make the solid content 40wt%.

[0087] 5. All liquid handling is carried out between 25-28°C. To 12.5 kg of the fibrillation mixture was added a mixture of 37.5 kg of ethanol and 200 mL of 12N hydrochloric acid (concentrated hydrochloric acid). Mix and stir for 5 hours, and centrifuge to make the solid content 50wt%.

[0088] 6. Add 31.7 kg of ethanol and 317 g of Tween 80 mixture to the above centrifuged mixture, mix and stir for ...

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