The present invention discloses rosa sterilis var. leioclada enzymes based on liquid fermentation and a preparation method thereof. The rosa sterilis var. leioclada is washed and cleaned, the cleaned rosa sterilis var. leioclada is cut into two halves, the cut rosa sterilis var. leioclada is subjected to a radiation sterilization, the sterilized rosa sterilis var. leioclada is transferred into a fermentation tank, 1-10 parts of edible honey, 80-100 parts of mountain spring water and 1-5 parts of fermenting micro-organisms are added, the mixture is subjected to an initial fermentation, and during the period, one stirring is conducted once a day; and after a still fermentation, the initially fermented products and the stand fermented products are mixed, the mixture is subjected to the still and closed fermentation, the temperature of a fermentation barrel is kept, the mixture is subjected to a natural fermentation, and the fermented mixture is filtered to obtain rosa sterilis var. leioclada enzyme fermented liquid. During the preparation process, the nutrients of the rosa sterilis var. leioclada enzyme raw materials are not lost or destroyed, so that the nutritional and health-care values of the prepared rosa sterilis var. leioclada enzymes are significantly improved. The rosa sterilis var. leioclada enzymes are simple in preparation technology, controllable in preparation processes, and stable in quality, can be stored at room temperature, are suitable for a large-scale production, and have good market prospects.