1755 results about "Aminobutyric acid" patented technology

The invention relates to a method for preparing health-benefiting fermented soybean meal enriched in small peptides, gamma-aminobutyric acid (GABA), reducing sugar, lactobacillus plantarum and antimicrobial peptide. By utilizing strains of aspergillus oryzae, saccharomyces cerevisiae, bacillus subtilis and lactobacillus plantarum, the small peptides, free amino acids, reducing sugar, GABA and high viable count content in the lactobacillus plantarum in the health-benefiting fermented soybean meal are obtained through the two-stage solid state fermentation method, the small peptides account for 8.01-18 percent, the free amino acids has the content of 80-300mg / g, the reducing sugar reaches 20-80mg / g, the GABA reaches 0.317-0.5mg / g, the viable count content in the lactobacillus plantarum is 2-6.5*1010cfu / g, the antibacterial titer of antimicrobial peptides is 2.0*103-7.0*104 IU / g, and the yield of the health-benefiting fermented soybean meal is 82.03-90 percent. According to the process, the nutrient effect of the soybean meal can be improved, the prepared fermented soybean meal has the antibacterial effect, partial antibiotics can be replaced, the fermented soybean meal is conveniently preserved and saved, the process is simple and feasible, and the fermented soybean meal is green and environment-friendly and has application significance and wide prospect in the feed industry and breeding industry.

The invention discloses a method for producing L-2-aminobutyric acid, and the method comprises the following step: catalyzing L-threonine utilized as a raw material through an enzyme catalysis system consisting of threonine deaminase, L-amino acid dehydrogenase and coenzyme regeneration systems, thus producing the L-2-aminobutyric acid. The method for producing theL-2-aminobutyric acid has the advantages that the raw material is low in price, the property is stable, and the production cost of the L-2-aminobutyric acid can be greatly lowered, the conversion rate and product concentricity are high, no influence caused by byproducts exists, and the method is suitable for industrialization application.

Fluorinated oligopeptides, especially those having 4,4-difluoro-2-amino butyric acid at the C terminus, may be effective inhibitors of hepatitis C virus NS3 protease. Examples of hexapeptides of the invention, optimized for binding in the S1 specificity pocket of the enzyme, may display IC50s at the sub-micromolar level. Embodiments of tripeptides of the invention, having a keto-acid group at the C-terminus are, likewise, potent inhibitors of NS3 protease.

The invention provides a novel high gamma-aminobutyric acid, high flavone fragrant black glutinous germinating brown rice and preparation thereof. The germinating brown rice is produced from material of long-shaped fragrant glutinous black rice, which has gamma-aminobutyric content 89mg / 100g, flavone content 8603mg / kg, and protein content 12.4%, wherein the content of amino acid and mineral substances is 3-5 times higher then the ordinary rice. The preparation includes material selecting, husk sieving, sterilizing, cleaning, immersing, sprouting, cleaning, terminating activity of enzyme, microwave drying, checking. The germinating brown rice has thick fragrance of popcorn, great mouth feeling, high content of gamma-aminobutyric acid, flavone and protein, high content of amino acid and mineral, high nutrition value. The germinating brown rice has reasonable materials, easy processing, enhances quality of germinating brown rice, prolongs storage period of the product, has obvious economic value, and provides technological guarantee to popularization of the germinating brown rice as a functional food.

The present invention relates generally to pharmaceutical dosage forms having immediate release and controlled release properties that contain a γ-aminobutyric acid (GABAB) receptor agonist, e.g., baclofen, for the treatment of medical conditions, which includes spasms, cramping, and tightness of muscles, associated with ailments such as multiple sclerosis or certain spinal injuries.

The process of biosynthesizing gamma-amino butyric acid includes the following steps: slant agar culture to activate Lactobacillus brevis with the preservation number of CGMCC No. 1306, inoculating to GYP or MRS seed culture medium to culture for 10-30 hr, inoculating to GYP or MRS fermenting culture medium in the amount of 0.5-5 % to stationarily culture at 25-35 deg.c for 48-120 hr to obtain fermented liquid containing thallus and centrifugally separating and collecting thallus; washing thallus with sterilized deionized water, suspending wet thallus in citric acid-disodium hydrogen phosphate buffering system with sodium L-glutamate to react for 1-10 hr, and centrifuging the reacted liquid to obtain gamma-amino butyric acid solution. The present invention has low production cost, mild reaction condition, less environmental pollution, simple process, high production efficiency, and high product purity.

The invention relates to biaryl-substituted 4-aminobutyric acid derivatives, and a preparation method and application thereof, particularly biaryl-substituted 4-aminobutyric acid derivatives disclosed as Formula (I), and a preparation method and application thereof, an intermediate of biaryl-substituted 4-aminobutyric acid derivatives prepared by cyclization reaction and a pharmaceutical composition containing the biaryl-substituted 4-aminobutyric acid derivatives. The biaryl-substituted 4-aminobutyric acid derivatives have NEP (neutral endopeptidase) inhibition activity, and can be used as an NEP inhibitor.

The invention discloses a preparation method and product of germinated wheat rich in gamma-aminobutyric acid, and the method is used for solving the problems of bad existing effect and unstable yield since GABA is enriched by utilizing tea and rice products according to an existing method for preparing gamma-aminobutyric acid based on a biological concentration method. The method comprises the following steps of: raw material selection, pretreatment, imbibition, temperature control training and germination termination. Wheat seeds are dramatically taken as raw materials, the improvement of GAD enzyme activity in wheat seeds is effectively promoted by controlling germination conditions, a cultivated substrate and the pH value, and meanwhile the GAD activity is ensured by controlling the pH value, the activity of GABA aminopherase is inhibited, and the content of gamma-aminobutyric acid in germinated wheat is greatly improved, so that the nutritive value of wheat is greatly improved. Experimental results show that the content of GABA in the prepared germinated wheat is 31.08 mg per 100 g at most, and achieves domestic and international leading level of similar methods.

The invention provides a sleep improvement composition. The sleep improvement composition is composed of gamma-aminobutyric acid, a spine date seed extract, magnesium and vitamin B6. The invention also provides a preparation of the sleep improvement composition and a preparation method of the sleep improvement composition. The sleep improvement composition contains no hypnotic components, and can effectively calm motion and improve the sleep for high-pressure populations and poor sleepers.

The invention discloses a health care food composition capable of assisting in improving memory, and a preparation method of the composition. The health care food composition contains docosahexenoic acid (DHA) algal oil, gamma-aminobutyric acid, phosphatidylserine, taurine, zinc gluconate, vitamin C and vitamin B12. The invention also discloses the preparation method of the health care food composition. The animal function test proves that the health care food composition has the function of assisting in improving memory, and is free from toxic and side effects.

The present invention relates generally to pharmaceutical dosage forms having immediate release and controlled release properties that contain a γ-aminobutyric acid (GABAB) receptor agonist, e.g., baclofen, for the treatment of medical conditions, which includes spasms, cramping, and tightness of muscles, associated with ailments such as multiple sclerosis or certain spinal injuries.

The invention relates to a substance for curing or relieving pain, and provides application of a gamma-aminobutyric acid A receptor inverse agonist containing alpha 5 subunits in preparing a drug for preventing and improving or curing the pain and also provides a drug composition containing the substance for curing or relieving the pain.

The invention relates to an L-2-reanal biological preparation method, which includes: utilizing L-threonine as raw materials, stirring the L-threonine in water at the temperature of 15-50 DEG C under the catalytic action of L-threonine deaminase and whole cells with a leucine dehydogenase and coenzyme regenerating function, and obtaining L-2-reanal after reaction. In the L-2-reanal biological preparation method, water is used for substituting for buffer salt solution to form a water-phase reaction system, cost of raw materials is reduced, threonine deaminase and whole cells used for catalyzing can be produced in batch by fermentation of microorganisms, are low in cost and wide in sources. Biological enzymatic catalysis reaction is water-phase reaction, transformation conditions of the enzymic method are mild, raw materials are transformed thoroughly, post-treatment is simple, products are separated by isoelectric point crystallization technology and film evaporation separation technology jointly, and the L-2-reanal biological preparation method is capable of recovering water and ammonium formate is low in cost, free of discharge of waste water and waste residues, environment-friendly in process and applicable to industrialized production of the L-2-reanal.

The invention relates to a glutamate decarboxylase mutant with improved thermal stability and an application thereof, belonging to the field of bioengineering. The mutant is prepared primarily by the following steps: performing saturated mutation of glutamine Q, valine V and threonine T on the sites 5-7 of the amino acid sequence of glutamate decarboxylase, and screening to obtain high-stability mutants Q5E / V6S / T7V, Q5Y / V6R / T7K and Q5N / V6Y / T7V. In the glutamate decarboxylase mutant prepared in the invention, the half-inactivation temperature is 45-50.5 DEG C which is 4.9-10.2 DEG C higher than that of wild type mutant; and the half-life period at 45 DEG C is 76-129min, which is 3.2-4.3 times higher than 24min of wild type mutant. By transforming glutamic acid for 12h with the whole cell of glutamate decarboxylase mutant synthesized from recombinant escherichia coli, 260-350g / L gamma-aminobutyric acid (GABA) can be obtained, and the molar yield is 76.6-97.8%. In the glutamate decarboxylase mutant prepared in the invention, the thermal stability is obviously improved, the production of gamma-aminobutyric acid is facilitated, and a foundation is laid for efficient synthesis of gamma-aminobutyric acid.

The invention relates to nutrient powder of germinated brown rice and a production process thereof and belongs to the food processing technology field. The invention is characterized in that the germinated brow rice is used as the main material is expanded, crushed, screened, mixed with oatmeal, soybean powder and white granulated sugar, sterilized and packaged, finally the nutrient powder of the germinated brown rice containing health ingredients of gamma-aminobutyric acid (GABA) is made. The germinated brown rice is produced by extrusion and expansion, so the invention has the advantages of no agglomeration and no precipitation during pouring the water into the nutrient powder, convenient use, being easily to be digested and absorbed by human body, and 15 to 20 mg per 100g GABA in the product.

The invention relates to a nanometer carboxylated polystyrene microsphere with a spacer arm and a preparation method thereof. A nanometer carboxylated polystyrene microsphere A is prepared by taking styrene and acrylic acid as a mixed monomer, taking water as a reaction medium, taking potassium persulfate as an initiator and taking sodium dodecyl sulfate as an emulsifier with an emulsion polymerization method. In the presence of EDC (Ethylidene Dichloride) serving as a coupling agent and Sulfo NHS, 4-aminobutyric acid, 6-amidocaproic acid or 8-aminocaprylic acid is connected to the surface of the microsphere, so that the nanometer carboxylated polystyrene microsphere with the spacer arm is prepared. The spacer arm is introduced into the surface of the nanometer microsphere, so that the steric hindrance of a coupling macromolecule is lowered, the activity and utilization ratio of the macromolecule are increased, and a nonspecific interaction between the macromolecule and the polystyrene microsphere is reduced. Meanwhile, the grain diameter of the microsphere can be adjusted by adjusting the using amount of the emulsifier, the length of the space arm and surface density, so that the nanometer microsphere has a wide application prospect in the fields of immune diagnosis, solid phase extraction and the like when serving as a carrier as well as a template.

The invention relates to a process for the preparation of 4-amino-1-hydroxybutylidene-1,1-bisphosphonic acid and of the trihydrated monosodium salt thereof, consisting of reacting 4-aminobutyric acid with a phosphonation mixture formed by phosphorous acid and methanesulfonic anhydride and thereafter hydrolyzing the product of said reaction and isolating the products by adjustment of the pH.

A cosmetic skin-care composition containing as the active principle a depigmentationally active combination of (a) an acidic mixture including (i) at least one alpha-hydroxylated acid or a derivative thereof, with the exception of ascorbic acid, and (ii) at least one compound selected from the group which consists of kojic acid, caffeic acid, azelaic acid, aminobutyric acid, fusaric acid, 5-hydroxy 2-hydroxymethyl-gamma-pyridone, and derivatives thereof, and (b) at least one active component of a plant extract from at least one plant selected from white mulberry, liquorice, skull cap, grapefruit, birch, heather, strawberry tree, bearberry, lemon, lettuce, oarweed, cucumber, ginseng, hop, sweet corn, feverfew, sage, soya, elder, spirulina, lime, ferocious aloe, yukinoshita, bloodwort, hoelen, wood rose, alpha-orizanol, burnet, ginkgo biloba, tanlex VB and Eclipsa alba, with the proviso that when the composition contains kojic acid and a liquorice extract, it contains at least one other plant extract component. The use of said composition for preparing a drug or in a cosmetic method is also disclosed.

The invention discloses nutritious sprouted glutinous coarse rice flour and a preparation process thereof. The nutritious sprouted glutinous coarse rice flour comprises 50-80% of sprouted glutinous coarse rice flour, 10-30% of buckwheat flour, 5-10% of bean flour and 5-10% of sesame flour; and the preparation process comprises the following steps of: sprouting at a selected proper temperature, spraying a mixed solution containing CaCl2 and monosodium glutamate during the sprouting operation to promote the enrichment of gamma-amino butyric acid (GABA) in sprouting, and finally, obtaining the nutritious sprouted glutinous coarse rice flour with GABA content of up to 20-40 mg / 100g, thereby supplementing the nutritious ingredients such as GABA and the like in the rice flour. The nutritious sprouted glutinous coarse rice flour plays roles in reducing blood pressure, improving brain function, calming nerves and promoting long-term memory; after being baked and cured, the nutritious sproutedglutinous coarse rice flour product has intense rice incense and good pasting performance and anti-caking performance. Due to the adoption of the nutritious sprouted glutinous coarse rice flour, the varieties and designs of the glutinous rice flour are enriched, and the utilization rate and the additional value of the glutinous coarse resources are increased.

Fermented soybean foods rich in gamma-aminobutyric acid are produced by fermentation of soybean using Tempe molds. Fermented soybean foods rich in gamma-aminobutyric acid and free amino acids are produced by fermentation of soybean using Tempe molds with anaerobic treatment. Fermented cereal foods rich in gamma-aminobutyric acid and free amino acids are also produced by fermentation of cereals with anaerobic treatment. Rhizopus oligosporus and Rhizopus oryzae are favorably used as the Tempe mold and Koji molds.

The invention belongs to the technical field of rice deep processing, in particular to a production method of a functional rice product which has a special sweet flavor and is rich in Gamma-aminobutyric acid and a product thereof, the rice product is produced by virtue of the activities of high glutamate decarboxylase, amylase and protease in germinated brown rice of an indica type rice or a non glutinous rice. The invention takes the indica type rice or the non glutinous brown rice as a raw material which carries out the steps of cleaning, disinfection, soaking, germination, cleaning, beating into rice milk, heat insulation, transformation, drum drying or spray drying, and the like, to be produced into functional nutrition rice flake or rice powder which is rich in Gamma-aminobutyric acid. Compared with the prior art, the rice product makes full use of the activities of the glutamate decarboxylase, the amylase and the protease in the germinated brown rice; the produced rice product has the special sweet flavor; by calculating based on dry basis, the content of Gamma-aminobutyric acid is high (1,000-1,400mg / 100g), and the nutritional value and the digestibility are high. The rice product of the invention can be taken as the functional food for resisting fatigue and reducing blood lipid.

A peptide of the formula IX-A-D-Trp-Ywherein X is hydrogen, glycine, alanine, leucine, isoleucine, valine, N-valine, proline, tyrosine, phenylalanine, tryptophan, D-alanine, D-leucine, D-isoleucine, D-valine, D-N-valine, D-proline, D-tyrosine, D-phenylalanine, D-tryptophan, gamma -aminobutyric acid or xi -aminocaproic acid; A is D-gluptamic acid or D-y-glutamic acid; and Y is glycine, alanine, leucine, isoleucine, valine, N-valine, proline, tyrosine, phenylalanine, tryptophan, D-alanine, D-leucine, D-isoleucine, D-valine, D-N-valine, D-proline, D-tyrosine, D-phenylalanine, D-tryptophn, gamma -aminobutyric acid, xi -aminocaproic acid, hydroxyl, or an amide group.

The invention discloses a germinated whole grain and a preparation method thereof, and belongs to the technical field of deep processing of grains. The whole grain is subjected to ultrasonic washing, high-voltage pulsed electric field treatment, biological enzymolysis or combined high-voltage pulsed electric field and biological enzymolysis treatment in a soaking process, and a segmented soaking and germination manner for germination is adopted, so that the sensory quality, germination yield and quality of a germinated whole grain product are improved, and germination loss is reduced. The preparation method is simple, low in soaking and germination temperature, short in cycle, energy-saving and environment-friendly. The content of functional, nutritional and bioactive substances in the germinated whole grain product is remarkably increased, and food safety is remarkably improved. For example, for brown rice, germinated brown rice prepared by the preparation method has gamma-aminobutyric acid content of 303.8 to 312.6 mg / 100 g, glutathione content of 16.8 to 18.3 mg / 100 g, IP6 content of 460.8 to 484.4 mg / 100 g and dietary fiber content of 3.5 to 3.6 mg / 100 g.

A nutritive health-care fruit juice is prepared through choosing Korean ganoderma as bacterial strain, preparing culture medium from germinated long-grained non-glutinous rice mild, malt juice and germinated soybean juice for deep ferment, slant culture, stage-one shaker culture, stage-two seeding tank culture, deep fermenting, mixing the fermented liquid with grape jice, apple juice and orange juice, homogenizing, degassing, sterilizing and cooling. Its advantage is rich bioactive substances (ganoderma polyose, gamma-aminobutanoic acid, etc).

The invention relates to a biosynthesized gamma-aminobutyric acid preparation. By weight percentage, the product comprises 5 percent to 60 percent of gamma-aminobutyric acid. The preparation method of the biosynthesized gamma-aminobutyric acid preparation includes the following steps: first, streptococcus lactis strains are inoculated to a seed medium for fermentation composed of glucose, corn steep powder, defatted soybean flour and monosodium glutamate, thus forming fermentation broth; the fermentation broth is drained into a high speed centrifuge to form clear liquid centrifugally; the clear liquid is added with 250 mg / L chitosan at the temperature of 40 DEG C, and stirred for flocculation; the flocculated fermentation broth to be filtered enters a plate-and-frame filter, thus obtaining filtered clear liquid; the filtered clear liquid passes a cationic resin exchange bed for ion exchange; when ion exchange resins are saturated, the liquid is eluted by deionized water to elute all the glutamate; ammonia is used for eluting the liquid and extracting the gamma-aminobutyric acid. The method realizes large scale production of functional food factor GABA, so the method adapts to the production and processing of food with various forms and specifications as well as GABA products of nutritional foods.

A method for preparing yoghourt rich in gamma-aminobutyric acid (GABA) relates to a method for preparing yoghourt and comprises the steps of resuscitating, purifying and activating lactobacillus plantarum NDC75017, lactobacillus bulgaricus 11057 and streptococcus thermophilus CICC 20379, respectively enlarging culture solutions and then mixing to obtain a leavening agent, and inoculating the leavening agent to sterilized milk to ferment. The GABA content in the yoghourt rich in GABA is 151.51mg / 100g. High-yield GABA bacterial strains and existing commonly-used bacterial strains are compoundedand then fermented to prepare the yoghourt rich in GABA, all the bacterial strains are food-grade lactic acid bacteria, and the safety is high. In addition, products have double effects, and the yoghourt rich in GABA integrates physiological efficacy of the GABA and health-care effect of yoghourt, namely the yoghourt rich in GABA is provided with special physiological efficacy of the GABA and thehealth-care effect of the yoghourt and is novel health-care yoghourt.

The invention discloses livestock and poultry feed added with germinated brown rice, which is characterized in that 1.3-3.2g of germinated brown rice powder is added into livestock and poultry feed per kilogram, and the germinated brown rice per hectogram contains 500-600mg of gamma-aminobutyric acid (GABA). The gamma-aminobutyric acid is replaced with the germinated brown rice in the invention; and the germinated brown rice contains abundant energy substances, protein and lipid oxidation resistant substances, such as inositol, ferulic acid, tocopherol, tocotrienol, oryzanol, phytic acid and the like in addition to providing GABA and contains abundant mineral substance elements and vitamin, such as calcium, magnesium, ferrum, zinc, vitamin B1, vitamin B2, vitamin E, niacin, pantothenic acid and the like. The livestock and poultry feed is better for the growth and development of animals.