The invention relates to a glycosylated
hemoglobin HbA1c freeze-
drying detection
reagent and a preparation method and application thereof.The preparation method comprises the steps that surface aminated
polystyrene microspheres are prepared through an
emulsion polymerization method, then double bonds are introduced through
Schiff base reaction and methylacrolein reaction, and the glycosylated
hemoglobin HbA1c freeze-
drying detection
reagent is obtained. Then preparing anionic
polystyrene microspheres with hydrophilic chains through free
radical polymerization, and preparing freeze-dried
powder of the HbA1c detection
reagent R1 by adopting the anionic
polystyrene microspheres; the preparation method comprises the following steps: preparing a hydrophilic cross-linking agent 2, 5-bis (2, 4-dihydroxy-6-formylphenoxy) terephthalaldehyde, then carrying out a
Schiff base reaction on the hydrophilic cross-linking agent 2, 5-bis (2, 4-dihydroxy-6-formylphenoxy) terephthalaldehyde and HbA1c mAb to prepare an HbA1c
oligomer, and preparing the HbA1c detection reagent R2 freeze-dried
powder by using the HbA1c
oligomer. The glycosylated
hemoglobin HbA1c freeze-
drying detection reagent prepared by the invention has the advantages of high
antigen binding efficiency, excellent detection sensitivity, sufficient retention of
antibody activity and strong detection specificity.