316 results about "Polystyrene microsphere" patented technology

The invention relates to the technical field of UV paint for floors, in particular to UV paint for SPC floors and a preparation method of the UV paint. The UV paint for the SPC floor is prepared from the following raw materials in parts by mass: 50 to 100 parts of UV resin, 20 to 40 parts of 1, 6-hexanediol diacrylate, 20 to 40 parts of a mixed solvent, 5 to 15 parts of polystyrene microspheres, 1 to 5 parts of tetraethoxysilane, 0.1 to 1 part of sodium dodecyl benzene sulfonate, 0.1 to 1 part of a silane coupling agent, 1 to 2 parts of diphenylmethane diisocyanate, 1 to 2 parts of a flatting agent, 1 to 2 parts of a photoinitiator and 1 to 2 parts of a delustering agent, the UV resin comprises urethane acrylate with the functionality of 2 to 3 and urethane acrylate with the functionality of 4 to 6. The paint film obtained by the invention has the advantages of high curing speed, strong adhesive force, good flexibility and excellent wear resistance, and is especially suitable for preparing SPC floors.

The invention belongs to the technical field of conductive microsphere preparation, and particularly relates to a surface-roughened nickel-plated conductive microsphere and a preparation method and application thereof. The preparation method comprises the following steps: carrying out pretreatment to enable the surfaces of the polymer microspheres to contain nickel ions, reducing the nickel ions into nickel nanoparticles, activating the microspheres and carrying out chemical plating. According to the invention, the specific surface area of the conductive microspheres is increased by reducing the nickel nanoparticles on the surfaces of the microspheres, a large amount of impurities cannot be generated among the microspheres, and adhesion among the microspheres can be effectively prevented. According to the prepared conductive microspheres, the plating layer and the polystyrene microspheres are firmly combined, and the risk that the conductivity is reduced due to the fact that the surface of the plating layer falls off is reduced. The preparation method is safe, environment-friendly, simple, convenient and suitable for industrial production requirements.

The invention relates to the technical field of in-vitro diagnostic kits, in particular to carboxyl polystyrene microspheres, a preparation method and application of the carboxyl polystyrene microspheres in a kit for quantitatively detecting MxA. The preparation method of the carboxyl polystyrene microspheres comprises the steps that firstly, polystyrene microspheres with the surface rich in aldehyde groups are prepared through a soap-free emulsion polymerization method; then carrying out a Schiff alkali reaction with 2-aminoethyl methacrylate hydrochloride to carry out double bond modification on the surface of the microsphere, and meanwhile, improving the hydrophilicity of the microsphere by an ester group and an imino group; and finally, carrying out sulfydryl-double bond addition reaction on the polystyrene microspheres and mercaptosuccinic acid to generate the polystyrene microspheres rich in carboxyl on the surfaces. The carboxyl polystyrene microspheres prepared by the invention can improve the binding efficiency of latex and an MxA antibody, so that the detection sensitivity is improved, the purpose of high-sensitivity quantitative detection of MxA protein can be achieved, and the detection time is greatly shortened.

The invention discloses cobalt molybdenum nitride / cobalt modified nitrogen-doped hollow carbon microspheres as well as preparation and application thereof, and relates to the technical field of electrochemical energy storage devices. Carboxylated polystyrene microspheres, soluble zinc salt and cobalt salt, phosphomolybdic acid and 2-methylimidazole are dispersed in methanol, a PMA (at) ZnCo-ZIFs / PS precursor of a spherical structure is obtained, then the PMA (at) ZnCo-ZIFs / PS precursor is placed in an inert atmosphere for carbonization treatment, and the cobalt molybdenum nitride / cobalt modified nitrogen-doped hollow carbon microsphere material is obtained. According to the structure and the preparation method, the nitride / metal cobalt component and the nitrogen-doped sodium affinity sites are introduced at the same time, uniform transportation and nucleation of sodium ions are effectively induced, sodium metal is guided to deposit in a specific three-dimensional structure, growth of sodium dendrites is effectively inhibited, volume expansion of metal sodium in the reaction process is relieved, the preparation method is simple and convenient, and the preparation method is suitable for industrial production. The cost is low, the electrochemical performance of the sodium metal battery assembled by using the composite material can be remarkably improved, and the composite material has a good large-scale application prospect.

The invention discloses a solid-phase microextraction derived fiber head and a preparation method and application thereof, the solid-phase microextraction derived fiber head comprises a solid-phase matrix and polystyrene microspheres adhered to the surface of the solid-phase matrix, and a chitosan cross-linked network layer is also cross-linked on the polystyrene microspheres; cysteine is also adsorbed on the polypropylene microsphere and chitosan cross-linked network layer. According to the solid-phase microextraction derivative fiber head, a large number of hydrophilic action sites such as amino groups and carboxyl groups exist on the surface, so that synchronous extraction and detection of the contents of three target analytes, namely cysteine-chlorothalonil association compounds, sulfydryl chlorothalonil and hydroxychlorothalonil, are realized.

The invention relates to the technical field of polycrystalline silicon system catalysts, in particular to a preparation method of a polycrystalline silicon catalyst for new energy, which comprises the following steps: preparing mesoporous polystyrene microspheres; the preparation method comprises the following steps: dispersing mesoporous polystyrene microspheres in a metal salt solution for precipitation reaction, dispersing the obtained supported polystyrene microspheres in ethanol, then adding stronger ammonia water and tetraethyl orthosilicate for stirring reaction to obtain supported silicon dioxide microspheres; and calcining in a carbon monoxide atmosphere to prepare the polycrystalline silicon system catalyst. According to the preparation method, loading and precipitation of metal salt are carried out by dissolving a large number of mesopores in polystyrene microspheres, then the polystyrene microspheres loaded with metal precipitation particles are fixed by a sol-gel method, and the interior and the surface of the generated silicon dioxide microspheres are mingled with the loaded polystyrene microspheres; and high-temperature reduction calcination is carried out in the atmosphere of carbon monoxide, so that the prepared polycrystalline silicon system catalyst has relatively high tetrachlorosilane conversion rate and long-time use stability.

This invention belongs to the field of biochip technology, specifically an erasable single nucleotide polymorphism (SNP) genotyping chip and its detection method. The erasable SNP genotyping chip provided by this invention is prepared using polystyrene microspheres, capture probes, and a silicon substrate. In the SNP recognition stage, it relies on a FEN1 enzyme-mediated invasive reaction. Based on the technical characteristics of this invasive reaction, downstream probes A and B are designed with self-protective hairpin structures, preventing false positive signals from hybridization with the capture probe even when added in excess, thus significantly improving detection specificity. The detection method of the erasable SNP genotyping chip provided by this invention overcomes the long-standing problem of high application costs caused by the single-use nature of solid-phase SNP gene detection chips.

The invention relates to the technical field of electronic gas purification, in particular to a silicon-based substituted polystyrene microsphere and a preparation method and application thereof, and the silicon-based substituted polystyrene microsphere comprises a crosslinked polystyrene skeleton; the cross-linked polystyrene skeleton is chemically grafted with a silicon group, and the silicon group is connected with a Si-Cl bond or a Si-Br bond. The silicon-based substituted polystyrene microspheres prepared by adopting an in-situ synthesis method are micron-sized highly-crosslinked solids, are stable in structure and are resistant to acid and alkali, and high-content Si-Cl bonds or Si-Br bonds are introduced into the silicon-based substituted polystyrene microspheres, so that the silicon-based substituted polystyrene microspheres react with water, an excellent water removal effect is achieved, new impurities are not generated, and the method is suitable for industrial production. Separation from electron gas is easy, and energy consumption is effectively reduced.

The invention belongs to the technical field of biochips, and particularly relates to a reusable genetic typing chip and a detection method thereof, the reusable genetic typing chip provided by the invention is prepared from polystyrene microspheres, capture probes and a silicon substrate, and depends on competitive hybridization of DNA in an SNP recognition stage, and the detection result is more accurate. The recognition sequence of the capture probe is converted into the sequence of the target object, and the added detection probes are hairpin probes with a self-protection function, so that the generation of false positive signals is remarkably reduced, and the detection specificity is remarkably improved. And no enzyme participates in the whole process, so that the detection process is remarkably simplified, and the detection cost is reduced.

The invention provides a preparation method of an exosome antibody microsphere conjugate, which belongs to the technical field of exosomes, and comprises the following steps: preparing a carboxyl polystyrene microsphere suspension with the particle size of 50 nanometers, and ensuring that the size of the microsphere is stabilized in a range of 50 + / -5 nanometers by optimizing centrifugation and cross-linking conditions. And then coupling the exosome marker antibody with the microspheres, and closing unreacted active groups. A mathematical model is adopted to optimize purification process parameters for purification. A dynamic light scattering and transmission electron microscope is utilized to characterize coupling physics properties, and a conjugate meeting requirements is screened. And finally, obtaining fluffy porous exosome antibody microsphere conjugate freeze-dried powder under the protection of a sucrose solution by adopting a vacuum freeze-drying method. The method solves the technical problems that in an existing exosome microsphere conjugate preparation process, centrifugation and purification parameters are often set according to artificial experience, optimization is difficult to obtain, and the quality of the exosome microsphere conjugate is affected.

The invention relates to the technical field of immunochromatography, in particular to time-resolved fluorescent microspheres, a preparation method and application of the time-resolved fluorescent microspheres in PSP / CRP / MxA quantitative detection immunochromatography test strip, and the preparation method of the time-resolved fluorescent microspheres comprises the following steps: 1) preparing surface aminated polystyrene microspheres; (2) carrying out glutaraldehyde crosslinking and 1, 10-phenanthroline-5-amino combination; 3) grafting a rare earth fluorescent dye on the surface; and 4) coating a polymer shell layer and modifying carboxyl. According to the invention, through a dual fixation strategy of surface grafting and polymer coating, the time-resolved fluorescent dye is confined to a functional region between the surface of the microsphere and a polymer shell layer, diffusion and leakage of dye molecules to the external environment are blocked, and the problem of dye shedding of traditional fluorescent microspheres is solved; the polymer shell layer can effectively isolate external ultraviolet light, oxygen and other corrosive factors, reduce photooxidative degradation and non-radiative transition loss of fluorescent molecules, and improve the light stability and fluorescent lifetime of the fluorescent molecules.

The invention relates to the technical field of lithium batteries, in particular to a preparation method of a positive plate and a battery comprising the positive plate. The invention discloses a positive plate preparation method and a battery comprising the positive plate, and the positive plate preparation method comprises the following steps: firstly, pre-mixing a pretreated NCM811 positive plate material, a composite conductive agent composed of super conductive carbon black and the like, and hydroxyl-containing modified polyvinylidene fluoride in proportion, adding polystyrene microspheres to prepare a slurry, coating a modified aluminum foil with the slurry, and carrying out vacuum drying to obtain a positive plate; performing segmented drying, gradient rolling and the like to obtain a positive plate; and finally, winding, packaging and injecting liquid according to the sequence of the positive plate, the diaphragm treated by dopamine and the negative plate, standing and forming to obtain the battery, and mainly improving the performances of conductivity, interface stability, low-temperature discharge and the like.

The invention discloses a preparation method of a composite phase change material, a thermal-insulation and antibacterial fabric and a preparation method thereof, and belongs to the field of functional textile fabrics and preparation thereof. The composite phase change material provided by the invention can be applied as a coating finishing component, and the preparation method comprises the following steps: taking polystyrene microspheres as a core, and forming a composite precursor through dopamine self-polymerization coating; and compounding the composite precursor with a layered double-metal hydroxide, and calcining to obtain the carbon microsphere supported layered double-metal oxide material with a hollow structure, and mixing the carbon microsphere supported layered bimetal oxide material with paraffin, and loading to obtain the carbon microsphere supported layered bimetal oxide material. The textile fiber fabric provided by the invention has excellent thermal insulation and antibacterial characteristics, is good in comfort, and has wide application prospects in industrial production and clothing application.

The invention relates to heat-insulating composite aerogel as well as a preparation method and application thereof. The heat-insulating aerogel comprises a framework silicon dioxide nanofiber, and a network interpenetrating porous structure formed by interweaving cellulose nanofiber and silicon dioxide nanofiber. Specific surface functionalization treatment is adopted to promote the interfacial compatibility between SiO2-NF and TOCNF, nano polystyrene microspheres are added, and finally annealing treatment is performed to precisely regulate and control the pores of the aerogel, so that uniform dispersion and network interpenetration of the aerogel in the nanoscale are realized; and the porous structure of the obtained composite network is effectively maintained by using a freeze-drying technology. The final product shows ultralow thermal conductivity, excellent lightweight characteristic and mechanical properties of rigidity and softness. The process is controllable, the raw materials can be selected from biomass, and the obtained aerogel has important application value in the fields of building energy-saving enclosure, aerospace thermal protection and the like.

The invention relates to the technical field of in-vitro diagnostic kits, in particular to hollow porous monodisperse carboxyl polystyrene microspheres, a preparation method and application of the hollow porous monodisperse carboxyl polystyrene microspheres in a kit for quantitatively detecting MxA. The preparation method of the microspheres comprises the steps that firstly, hydrophilic polystyrene microspheres are prepared to serve as stable particles; then fully mixing the Pickering particles with a first oil phase to form Pickering stable particles; then a surfactant and a second oil phase are added, the mixed system is stirred at a high speed, and the second oil phase permeates into the surfaces or pores of Pickering stable particles through interface interaction and molecular diffusion; under the action of an initiator, the polymeric monomers are subjected to a polymerization reaction, the low-boiling-point organic solvent is evaporated out along with temperature rise, and a polymer with a core part of a hollow structure and a shell layer of a porous structure is formed; and adding a carboxyl-containing functional monomer to form a hydrophilic polymer chain on the surface of the polymer, thereby finally obtaining the hollow porous monodisperse carboxyl polystyrene microspheres.

The invention discloses a silver-based electromagnetic shielding coating layer and a preparation method thereof, and belongs to the technical field of electromagnetic shielding materials. The coating layer comprises the following components in parts by mass: 30-50 parts of composite modified polystyrene silver-loaded microspheres, 80-150 parts of epoxy resin, 15-40 parts of a polyether amine epoxy curing agent, 0.5-2 parts of a defoaming agent and 0.5-2 parts of a flatting agent, and the coating layer is obtained by mixing all the components, coating the insulating carrier with the mixture and curing. The composite modified polystyrene silver-loaded microspheres are prepared by modifying sulfhydrylated polystyrene microspheres with trimethylpropane monoallyl ester, modifying with histidine, quaternizing, loading with phosphotungstic heteropoly acid and carrying out reduction loading with silver ions. The phosphotungstic heteropoly acid anchors silver ions to form a uniform and compact silver layer, and the silver-loaded microspheres form a three-dimensional conductive network to ensure the shielding effectiveness; the organic modified layer and the epoxy resin matrix form strong interface combination to avoid agglomeration and sedimentation; the oxidation resistance is improved through a multi-barrier structure, the quaternary ammonium salt has the antibacterial property, and high conductivity, excellent oxidation resistance, good matrix compatibility and shielding stability are achieved.

The invention relates to the technical field of micro-fluidic chips, and discloses a multi-fluid effect synergistic three-dimensional single-row focusing micro-fluidic chip and a method for single-line focusing of polystyrene microspheres by using the micro-fluidic chip, the micro-fluidic chip comprises a sample liquid inlet, one end of the sample liquid inlet is provided with an inertia focusing link, and the other end of the sample liquid inlet is provided with an inertia focusing link; a tapered flow guide cone structure is arranged between the inertial focusing link and the sample liquid inlet and is used for inhibiting the initial dispersion and diffusion trend of particles at the sample liquid inlet; the bionic gradient height structure is positioned at one end, far away from the tapered flow guide cone structure, of the inertial focusing link and is used for focusing and compressing the particles in the vertical direction; a sheath fluid focusing area is arranged at one end, far away from the inertial focusing link, of the bionic gradient height structure, and a sheath fluid inlet and a chip mixed liquid outlet are sequentially formed in one end, far away from the bionic gradient height structure, of the sheath fluid focusing area. According to the invention, efficient three-dimensional centered arrangement is realized, dynamic convergence of a particle trajectory from a dispersion state to a central single beam is realized, and a digital focusing regulation capability is realized.

The present invention discloses an additive manufacturing method for a laser window with a micro-nano structure and a laser window. The steps include: 1) ultrasonically cleaning the polished quartz glass with a chemical reagent; (2) self-assembling polystyrene microspheres using a micro-injection pump and transferring them onto the ultrasonically cleaned quartz glass; (3) reducing the diameter of the polystyrene microspheres using an ion beam assisted coating technique and then coating a silica material thereon; (4) cleaning the quartz substrate with a chemical reagent to wash away the polystyrene microspheres with a part of silica; (5) performing secondary ion beam assisted coating on the cleaned quartz substrate. The laser window with a micro-nano structure prepared by this method has a high transmittance, a wide anti-reflection band, and strong laser damage resistance.

The invention discloses a preparation method and application of high-sensitivity and high-stability time-resolved fluorescent microspheres, and relates to the technical field of immunochromatography. The preparation method comprises the following steps: (1) preparing high-stability high-carboxyl-density polystyrene microspheres; (2) preparing a high-stability europium ion chelate; and (3) preparing the high-stability fluorescent microspheres. Through a strategy of combining functional monomer copolymerization and surface covalent labeling, the fluorescent microspheres which are connected through strong covalent bonds from inside to outside and are high in stability and carboxyl density are constructed; obvious differential advantages and innovativeness are shown in the aspects of chemical stability, anti-leakage capability of fluorescent markers and surface functional group density. A time-resolved fluorescent microsphere test strip developed based on the microspheres has the advantages of good stability, high detection sensitivity and the like, and can realize rapid detection and analysis of a substance to be detected.

The invention relates to the field of preparation of calcium silicate veneers, in particular to a high-performance calcium silicate building veneer and a preparation method thereof. The invention provides a compact calcium silicate veneer with a multi-layer composite structure, which is formed by adding chemical raw materials with different components in a layered manner. The used innovative sound insulation material is a microporous calcium silicate-serpentine fiber-polystyrene composite material. By adding polystyrene microspheres and serpentine fibers in a specific proportion into calcium silicate, a porous structure with an excellent sound absorption effect is formed. The material not only has remarkable sound insulation performance, but also can effectively improve the waterproofness and weather resistance of the board and prolong the service life of the board.

The invention provides a method for preparing micron-sized polystyrene microspheres by an improved dispersion polymerization method. The method provides three large-size microsphere preparation regulation strategies: 1) a regulator adding strategy: particle size control is realized by regulating the solubility of an initial long chain of polystyrene; 2) a dynamic solvent concentration control strategy, wherein the growth of the microspheres is influenced by adjusting the dynamic concentration of styrene in the system; and 3) an oxygen introduction strategy, wherein the final particle size is regulated by controlling the number of primary nuclei. The method has the characteristics of simple process and good repeatability, and the particle diameter of the prepared polystyrene microspheres is gt; and after multiple times of washing, precipitation and separation, the coefficient of variation (Cv) is less than 5%.

The application discloses an aggregation-induced emission microsphere with colorimetric-fluorescent double signals and a preparation method and application thereof. The aggregation-induced emission microsphere comprises carboxyl polystyrene microspheres and aggregation-induced emission dyes embedded in the carboxyl polystyrene microspheres; and the structural formula of the aggregation-induced emission dyes is as follows: The aggregation-induced emission microsphere has colorimetric-fluorescent double signals, is red under natural light, has strong absorption at 600 nm, and shows high light-emitting efficiency at 725 nm, effectively avoids the interference of biological autofluorescence, reduces background interference, has good light stability and light bleaching resistance, and has high molar extinction coefficient, so that the aggregation-induced emission microsphere has colorimetric and fluorescent double signal output capabilities. In addition, the preparation process of the aggregation-induced emission microsphere has high reproducibility, has the potential for large-scale synthesis, and meets the needs of industrial batch production.

The invention belongs to the technical field of post-processing of silicon carbide wafers, and particularly relates to a polishing solution for polishing a silicon carbide wafer and a preparation method of the polishing solution. The polishing solution for polishing the silicon carbide wafer comprises the following components in percentage by mass: 5-30% of a grinding material, 3-8% of an oxidizing agent, 1-5% of a slow-release ionic liquid, 0.05-1% of a dispersing agent, 0.05-1% of a pH regulator and the balance of deionized water, the slow-release ionic liquid is ester-based ionic liquid grafted by amino polystyrene microspheres. According to the invention, the slow-release ionic liquid is creatively used, so that the damage of the abrasive to the silicon carbide wafer is avoided, the roughness is reduced, and the polishing rate is improved.

The invention belongs to the technical field of conductive materials, and particularly relates to a method for preparing and activating pyridine functionalized swelling polymer microspheres and a method for preparing core-shell structure polymer conductive microspheres. The preparation method comprises the following steps: by taking polystyrene microspheres as seeds, preparing pyridine functionalized polymer microspheres through an emulsion swelling method, then introducing Pd < 2 + > ions through coordination, preparing activated microspheres with palladium nanoparticles uniformly coated on the surfaces of the polymer microspheres through in-situ reduction, preparing nickel-plated microspheres with uniform metal coatings through chemical plating, and finally preparing the polymer microspheres with uniform metal coatings. And finally, preparing the core-shell structure gold-plated polymer conductive microspheres through in-situ reduction, and applying the core-shell structure gold-plated polymer conductive microspheres to preparation of anisotropic conductive films. Through an emulsion swelling method, not only can the polymer microspheres with adjustable sizes be prepared, but also the polymer microspheres with different morphologies can be prepared. The preparation method of the conductive microspheres has the advantages of being wide in raw material source, easy and convenient to operate and adjustable in metal coating thickness, and is suitable for high-precision packaging in the microelectronic industry.

The application discloses a kind of " sandwich type " crosslinked polystyrene analysis magnetic beads based on swelling and layer-by-layer assembly and its preparation method and application, the preparation method of magnetic bead is: with carboxylated crosslinked polystyrene microspheres as template, Fe3O4 is loaded to the microsphere template of swelling and forms microsphere assembly;Then in containing PEI poor solvent, assemble oleic acid modified hydrophobic Fe3O4;After that, microsphere assembly is phase transfer from organic phase to water phase, then grow silica shell layer, finally sequentially modified and carboxylated by amino, obtain carboxylated " sandwich type " crosslinked polystyrene analysis magnetic bead.The application uses carboxylated crosslinked polystyrene microspheres as template, loads Fe3O4 particle by swelling method and layer-by-layer self-assembly method, realizes biocompatibility by microsphere outer package silicon, provides a kind of analysis magnetic bead with uniform size, fast magnetic separation speed, slow settling time, good biocompatibility, good detection performance, applied to the detection combined with ELISA has good technical effect.

An RNA room-temperature storage stabilizer, an RNA product, and a preparation method and use thereof are disclosed, belonging to the field of biotechnology. The RNA room-temperature storage stabilizer provided by the present disclosure includes a functionalized polystyrene microsphere. The RNA room-temperature storage stabilizer has good adsorption capacity for RNA. The functional groups on the RNA room-temperature storage stabilizer can interact with the groups in the RNA, which plays a role in stabilizing the structure of the RNA, thereby realizing long-term stable storage of the RNA at room temperature without the need of introducing an ultra-low temperature device and other protective reagents; and the addition of water can destroy the binding between the functionalized polystyrene microsphere and the RNA, so as to achieve complete separation of the RNA from the stabilizer. It has the advantages of low cost, simple operation and high biosafety.

The invention discloses a white polyester film for compounding building decoration materials and a preparation method and application of the white polyester film for compounding the building decoration materials. The white polyester film is of a three-layer co-extrusion structure and comprises a core layer and two surface layers, comprising the following components in percentage by mass: 8%-20% of polystyrene microspheres with the particle size of 0.5-10 microns, 50%-70% of PET polyester chips with the titanium dioxide content of 3000ppm and 20%-40% of PET polyester chips with the silicon dioxide content of 150000ppm. The surface layer A is a low-gloss surface layer and comprises the following components in percentage by mass: 50%-70% of PET polyester chips with the silicon dioxide content of 30000 ppm and 30%-50% of PET polyester chips with the silicon dioxide content of 15000 ppm; and the surface layer C comprises the following components in percentage by mass: 10%-40% of PET polyester chips with the silicon dioxide content of 30000 ppm and 60%-90% of PET polyester chips. The white polyester film has a good shaping effect after being compounded and stamped with the plate, and does not yellow after being irradiated by a UV lamp for 60 hours.

The application belongs to the technical field of honeycomb active carbon production, and particularly relates to a titanium modified honeycomb active carbon composite material, a preparation method and application thereof. The titanium modified honeycomb active carbon composite material is prepared by the following steps: mixing tetrabutyl titanate, acetylacetone and anhydrous ethanol, adding a dispersing agent and stirring to obtain a titanium dioxide sol; dipping the honeycomb active carbon in an alkali solution, washing, drying to obtain a substrate; vacuum negative pressure dipping the substrate in the sol; introducing inert gas to calcine the substrate to form an amorphous TiO2 layer; introducing ammonia gas to calcine to form nitrogen-doped TiO2; dipping the calcined substrate in F127 n-propanol solution, spraying an ethanol suspension containing polystyrene microspheres after the F127 n-propanol solution volatilizes, and calcining in inert gas to form through pores. The composite material provided by the application has excellent adsorption performance and high specific surface area, and can be used in the fields of air purification, industrial waste gas treatment, water body remediation and the like.

The invention provides a multicolor flow-based synaptic heterogeneity analysis device and analysis method and application thereof, the analysis device comprises: (1) a scattering-resolution pretreatment module: adopting a BD FACS fusion double laser platform, firstly positioning an FSC-SSC coordinate by using a standard 0.5 m polystyrene microsphere, and then loading a synaptic body; the adopted sheath fluid comprises HPMC (Hydroxymethyl Methacrylate)-3cp and 1 * PBS (Phosphate Buffer Solution) without Ca < 2 + > / Mg < 2 + >; an adopted carrier buffer solution comprises EDTA (Ethylene Diamine Tetraacetic Acid), a 1 * protease inhibitor mixture and 1 * PBS (Phosphate Buffer Solution) without Ca < 2 + > / Mg < 2 + >; and (2) a multi-color flow type synchronous marking-sorting module which is used for detecting two kinds of code fluorescent marks in parallel on double laser platforms of 488 nm and 561 nm, eliminating cross color by a logic gate-double exponential decay compensation algorithm, and realizing transmitter and function sorting.

The invention relates to a glycosylated hemoglobin HbA1c freeze-drying detection reagent and a preparation method and application thereof.The preparation method comprises the steps that surface aminated polystyrene microspheres are prepared through an emulsion polymerization method, then double bonds are introduced through Schiff base reaction and methylacrolein reaction, and the glycosylated hemoglobin HbA1c freeze-drying detection reagent is obtained. Then preparing anionic polystyrene microspheres with hydrophilic chains through free radical polymerization, and preparing freeze-dried powder of the HbA1c detection reagent R1 by adopting the anionic polystyrene microspheres; the preparation method comprises the following steps: preparing a hydrophilic cross-linking agent 2, 5-bis (2, 4-dihydroxy-6-formylphenoxy) terephthalaldehyde, then carrying out a Schiff base reaction on the hydrophilic cross-linking agent 2, 5-bis (2, 4-dihydroxy-6-formylphenoxy) terephthalaldehyde and HbA1c mAb to prepare an HbA1c oligomer, and preparing the HbA1c detection reagent R2 freeze-dried powder by using the HbA1c oligomer. The glycosylated hemoglobin HbA1c freeze-drying detection reagent prepared by the invention has the advantages of high antigen binding efficiency, excellent detection sensitivity, sufficient retention of antibody activity and strong detection specificity.