31 results about "Salmonella antibody" patented technology

The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU/mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU/mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.

A fluorescent fiber-optic biosensor system using ultrasonic concentration of particles and cells for the detection of Salmonella typhimurium. A biosensor test chamber serves as an ultrasonic standing-wave cell that allows microspheres or cells to be concentrated in parallel layers or in a column along the axis of the cell. A fiber probe along the axis delivers laser excitation to fluorescent-labeled antibodies of Salmonella and collects the fluorescent signal. The Salmonella-antibody complexes are moved acoustically to the axis of the cell, increasing the fluorescent signal. Alternatively, the Salmonella-labelled antibody complexes attach to unlabeled antibodies that have been immobilized on the surface of polystyrene microspheres. This entire structure can be manipulated acoustically and the increase in the fluorescent signal, which can be an order of magnitude, indicates the presence of Salmonella.

An ELISA kit for detecting a Salmonella pullorum antibody is established by screening Salmonella pullorum dominant antigen GroEL through an immunoprecipitation technology, expressing GroEL recombinant protein through utilizing a prokaryotic expression vector, and utilizing an antigen protein. The kit can reduce the response of chicken in the detection process of the Salmonella pullorum antibody, and can improve the detection specificity and the sensitivity.

The invention discloses an ELISA kit for detecting a salmonella antibody. The kit comprises a solid-phase carrier coated with recombinant protein PagC, an enzyme-labeled antibody, salmonella negative serum and positive serum. The ELISA kit and method for detecting the salmonella antibody can be widely applied to salmonella, and application range is wide. The kit has high specificity and repeatability, the influence of temperature on reaction plates is small, and stability is high. Compared with an ELISA method with polysaccharide antigen as detection antigen, the method has the advantages that the occurrence rate of false positivity can be reduced to the maximum, and interference of other bacterial antigens in enterobacteriaceae on detection is avoided. Compared with a slide agglutination antigen detection method mostly adopted clinically, the method has the advantages that sensitivity and accuracy are higher, and detection throughput is increased greatly.

The invention provides a kit used for detecting swine salmonella antibody. The kit comprises antigen protein; the antigen protein is fljB protein, and the amino acid sequence of the antigen protein isrepresented by SEQ ID NO.3. The fljB protein is excellent in antigenicity, and high in specificity, purity, and yield; in the kit, the antigen protein coating concentration is 20<mu>g/ml, and negative and positive serum dilution ratio is 1:200. The kit can be used for swine salmonella fljB antibody rapid detection of clinical samples, is capable of solving a problem in the prior art that there isno single antigen salmonella infection diagnosis method, reducing failure detection rate, and increasing precision. A sensitive detection method is provided for swine salmonella epidemiological investigation in China, and the kit can be used for early diagnosis of swine salmonella infection.

The invention discloses a non-diagnostic purpose method for rapidly detecting Salmonella pullorum and Salmonella gallinarum. The detection method comprises the following steps: preparing Salmonella pullorum and Salmonella gallinarum antibody labeled coupled magnetic nano-particles (IgG-MNPs) and antibody and enzyme double labeled silica nano-particles (GOx-IgG-SiNPs), forming an immune compound IgG-MNPs/S having a sandwich structure, S. Pullorum and S. Gallinarum GOx-IgG-SiNPs, when the target bacteria to be detected exist, performing an enzymatically catalyzed reaction, allowing the obtainedreaction product and a color developer to form a complex product, and detecting the absorbance value of the obtained system to obtain the concentrations of the Salmonella pullorum and Salmonella gallinarum in order to realize the quantitative detection of the Salmonella pullorum and Salmonella gallinarum. A cascade reaction triggered by glucose oxidase (GOx) is combined with an Fe<3+>-SCN<-> colordevelopment system to realize the quantitative detection of Salmonella pullorum and Salmonella typhimurium. The detection method has the advantages of convenience, fastness, easiness in operation, good sensitivity, good specificity and good stability.

The invention relates to the technical field of biology and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. An antigencombination of the kit comprises p27 protein, gp85 protein and GroEL-delta8-1 protein. Avian leukosis virus capsid protein p27, prokaryotic expression protein of envelope protein gp85, and prokaryoticexpression protein of truncated GroEL-delta8-1 of a salmonella pullorum dominant antigen are used as a coating antigen to develop an ELISA (Enzyme-linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorum antibody. Compared with a current common kit for independently detecting avian leukosis or pullorum disease, the kit provided by the invention has the effect of simultaneously detecting the avian leukosis or the pullorum disease, and the detection and purification work of the avian leukosis and the pullorum disease can be extremely alleviated.

The invention relates to the technical field of animal epidemic disease detection and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. The kit comprises: P27 protein, GroEL-delta8-1 protein, an enzyme labeled second antibody, BSA (Bovine Serum Albumin), coating liquid, confining liquid, a color developing solution anda stopping solution. The invention develops an indirect ELISA (Enzyme Linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorumantibody by taking prokaryotic expression protein of a conserved region P27 of an avian leukosis virus and prokaryotic expression protein of truncated protamine GroEL-delta8-1 of a dominant antigen GroEL of salmonella pullorum as coating antigens; the kit provided by the invention can be used for simultaneously detecting avian leucosis and pullorosis; chickens which are detected to be positive areeliminated so that the aim of simultaneously purifying is realized and the clinical detection and purification work is extremely alleviated.

The invention discloses a salmonella spp antibody latex agglutination detection method belonging to the technical field of immunology. The method comprises the following steps: connecting a SEN4030a protein onto a blank latex microsphere, and preparing a sensitization latex microsphere; enabling the sensitization latex microsphere to react with a sample to be detected, and observing a agglutination result. According to the method, the SEN4030a protein is used as an antigen to prepare sensitization latex particles to detect the salmonella spp infection antibody. The SEN4030a protein has higher specificity and immunogenicity, and the prepared sensitization latex has no self-agglutination phenomenon and is high in specificity, good in repetition and stable in properties. The method provided by the invention is rapid, accurate, simple and easy; and compared with the commercialization detection antigen, the accuracy is relatively high.

The invention discloses a detection card for detecting Salmonella enteritidis in tableware. The detection card comprises a sample liquid absorption part, a baseboard, a gold marked salmonella antibody layer, a detection reaction part and a water absorption part, and the sample liquid absorption part gold marked salmonella antibody layer, the detection reaction part and the water absorption part are sequentially applied to the back lining of the baseboard from the left to the right. The detection card for detecting the residual condition of the Salmonella enteritidis in tableware has the advantages of high sensitivity and sensitivity, simplicity in operation, and no special facilities. The detection card has high specificity and sensitivity, is rapid, fast and convenient to operate in the detection process, and is suitable for being used in clinic examination, epidemiologic investigation and place quarantine. The detection card has the advantages of simple preparation method, good stability and good repeatability.

The invention belongs to the field of animal husbandry and veterinary and in particular relates to a method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of the method. The method comprises the following steps: feeding breeder flocks in single cages and encoding; collecting primary hatching eggs of the breeder flocks; enabling the primary hatching eggs to correspond to numbers of the hatching eggs one by one; disinfecting the surfaces of the hatching eggs and opening egg shells from hatching egg air chamber ends;sucking egg white and yolks and putting into PE (Polyethylene) tubes respectively and marking; putting egg white and yolk samples into a refrigerator and freezing and thawing for a plurality of times; taking the frozen and thawed egg white; detecting an ALV virus antigen through an avian leukosis ELISA (Enzyme Linked Immunosorbent Assay) detection method; taking the frozen and thawed yolks and adding normal saline and chloroform; after uniformly mixing on an oscillator, centrifuging and layering a solution, wherein a lower layer is an organic solvent, a middle layer is denatured lipid proteinand an upper layer is an extracted antibody; and sucking the antibody of the upper layer and detecting a salmonellosis antibody through a plate agglutination method; and eliminating chicken flock individuals with positive detection results of the ALV virus antigen and the salmonellosis antibody.

The invention discloses a pullorum disease antibody latex agglutination negative selection detection kit, comprising a salmonella surface protein InvJ recombinant protein sensitized latex reagent and a secreted protein SopA C-terminal recombinant protein sensitized latex reagent, wherein the InvJ recombinant protein sensitized latex reagent can rapidly detect a salmonella antibody by virtue of a latex agglutination test, the SopA C-terminal recombinant protein sensitized latex reagent can detect serotype salmonella antibodies except for the pullorum disease by virtue of the latex agglutination test, and the combination of the two reagents is used for negative selection of a pullorum disease antibody positive blood or serum sample. The invention also discloses a preparation method of salmonella surface protein InvJ recombinant protein and secreted protein SopA C-terminal recombinant protein sensitized latex reagents. The pullorum disease antibody latex agglutination negative selection detection kit disclosed by the invention has the advantages of stable antigen, strong specificity and high sensitivity, and solves the problem of antigen instability caused by potential variability and changed culture conditions of an existing pullorum disease agglutination antigen production strain.

The invention relates to the technical field of sterile egg production, in particular to a sterile egg production process aiming at salmonella, which comprises the following steps: firstly, carrying out salmonella antibody-containing breeding on female chickens, then optimizing the breeding environment of laying hens, namely, breeding the laying hens in different houses, and regularly detecting and killing the breeding farm of the laying hens; laying hens are fed with feed containing a selenium-rich additive and a mould synergistic leavening agent, the laying hens with negative salmonella are obtained, then a breeding farm of the laying hens is subjected to high-intensity sterilization to prevent vertical propagation and horizontal propagation of salmonella, the laid eggs are placed in a clean and sterile vessel, then the surfaces of the eggs are treated, and the laying hens with the negative salmonella are obtained. And finally obtaining the salmonella-free fresh eggs. According to the method, the detection rate of salmonella in the sterile eggs is remarkably reduced, the operation is simple, the breeding cost is saved, and commercial application is facilitated.

The invention relates to an ELISA kit for detecting salmonella antibody, a detection method and application thereof, belonging to the technical field of microbial immunology. The ELISA kit comprises antigen protein or antigen protein solution or a coated plate coated with the antigen protein solution, an enzyme-labeled antibody, color developing solution, stop solution, positive serum and negativeserum. The kit can quickly, specifically and sensitively detect the salmonella antibody in the serum, and the sensitivity of detecting the serum antibody is 1: 1000; and the kit has simple and convenient operation, low cost, easy observation of reaction result, high sensitivity and strong specificity, and is applicable to the technical fields of salmonella monitoring, epidemiological investigation, clinical sample detection and the like.

The invention discloses a cart for detecting a salmonella antibody in a henhouse. The cart comprises a frame and a box body, wherein the frame is provided with a box body supporting plate and wheels; the box body supporting plate is arranged at the upper part of the frame and is used for supporting the box body; the wheels are arranged below the frame; the box body is a box-shaped component; the top surface of the box body is transparent; a lamp holder is mounted in the internal space of the box body and is used for holding a bulb. An incandescent bulb or an energy-saving lamp can be mounted on the lamp holder according to the seasons, an appropriate environment temperature is provided for a detection material placed on the top surface, a light source is stable, and observation is facilitated.

The invention discloses a method and detection kit for rapidly and synchronously detecting the total number of salmonella and bacteria in a sample in multiple times. A red fluorescent probe capable ofmarking all bacteria is used for marking the total number of bacteria to distinguish the bacteria and other background particles in the sample, and meanwhile, by a green fluorescent probe, a salmonella antibody cross-linked through a chemical group is used for distinguishing salmonella and non-salmonella in the sample; and red and green fluorescence signals are simultaneously counted through a flow analyzer, so that the synchronous detection of the total number of salmonella and bacteria is realized. The method can be used for simultaneously detecting the total number of salmonella and bacteria, is simple and convenient to operate and short in consumed time, and can be used for detecting most samples within 0.5 hour.

The invention discloses a preparation method and a screening method of a salmonella PagC protein monoclonal antibody. The preparation method comprises the following steps of immunizing Balb/c mice byusing purified PagC protein, and obtaining six hybridoma cells capable of producing an anti-PagC protein monoclonal antibody according to a hybridoma cell preparation technology; and synthesizing intergeneric specific amino acid sequences DRQASGSVEPEGIH(P1) and FKEHSTQDGDSFNKISSRKTGFA(P2) containing linear epitopes as screening antigens to obtain a monoclonal antibody cell strain J of which the antigen epitopes are on a linear P1 sequence, wherein the other five monoclonal antibody epitopes are not on linear P1 and P2 amino acid sequences. Western-blotting results show that mAb J can react with purified PagC protein, wild type pullorum disease and mouse typhoid fever PagC protein and does not react with other enterobacteriaceae PagC protein, and it is shown that the screened mAb J epitopeP1 is conservative in salmonella and is intergeneric specific. By preparing the monoclonal antibody, a salmonella antibody blocking ELISA detection method can be established, and the monoclonal antibody has the characteristics of good specificity and high sensitivity and has wide popularization potential.