43 results about "Salmonella enteritidis" patented technology

The present invention belongs to the field of biotechnology and relates to a five - plex fluorescence PCR detection reagent for Salmonella, Salmonella enteritidis, Salmonella typhimurium, Salmonella pullorum and Salmonella gallinarum and its application. By using the five - plex fluorescence PCR detection reagent of the present invention, the purpose of simultaneously identifying and detecting Salmonella enteritidis, Salmonella typhimurium, Salmonella pullorum and Salmonella gallinarum can be achieved. It has the advantages of simple operation, rapidity, high accuracy, high sensitivity and good repeatability, and is of great significance for the rapid and accurate detection of clinical animal salmonellosis and epidemic disease prevention and control.

The invention provides a strain of high-temperature-resistant and inflammation-relieving Witzia ciliaris and an application thereof, the high-temperature-resistant and inflammation-relieving Witzia ciliaris is Witzia ciliaris CN985 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is April 7, 2024, and the preservation number is CCTCC NO: M 2024641. The invention further provides a preparation method of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain. The invention provides a brand new strain of Witzia ciliaris CN985, which is resistant to high temperature, has better acid resistance and bile resistance, and has no hemolysis risk when being applied to a human body. The Witzia coagulans CN985 has excellent antibacterial performance, and especially can significantly inhibit streptococcus pyogenes, salmonella enteritidis and / or group B streptococcus; besides, experimental results show that the Witzia ciliaris CN985 can relieve enteritis, improve the immunity of the organism and relieve pharyngitis, and especially when the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 are combined for use, the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 have a synergistic interaction effect, so that the relieving effect on enteritis can be remarkably improved.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LM2 and application of the lactobacillus plantarum strain LM2. The strain has relatively high GABA (gamma-aminobutyric acid) production capacity and nitrite degradation capacity, has a bacteriostatic effect on intestinal pathogens such as staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and campylobacter jejuni, has a good bacteriostatic function, also has good acid resistance and cholate resistance, and can be used for preparing a bacterial strain for treating intestinal diseases. The strain can well survive in artificial intestinal juice and artificial gastric juice; in order to increase the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LM2 is obtained by researching the accelerant, and the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 99% or above, the yield of GABA is increased to 3.0 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

This invention belongs to the field of molecular biology detection technology, specifically relating to a primer and probe combination for detecting avian Salmonella based on the RAA-LFD method and its application, particularly to a primer and probe combination for detecting three avian serotypes of Salmonella: Salmonella pullorum, Salmonella enteritidis, and Salmonella typhimurium, based on the RAA-LFD method and its application. This invention designs different types of probes and primers targeting the genes of serotypes SP, SE, and STM of Salmonella. Using the primer and probe combination of this invention, rapid, sensitive, and specific detection of Salmonella serotypes and the three avian Salmonella serotypes SP, SE, and STM can be achieved, greatly shortening the detection cycle of avian salmonellosis and improving the detection efficiency and accuracy of avian salmonellosis.

The application discloses application of T1FR protein in inhibition of Klebsiella pneumoniae and Salmonella enteritidis type I fimbriae, and relates to the field of biotechnology. T1fr The biological factor T1FR protein encoded by the gene can inhibit Klebsiella pneumoniae and Salmonella enteritidis type I fimbriae adhesion factors, and can effectively reduce the use of antibiotics and relieve the drug resistance of Klebsiella pneumoniae and Salmonella by inhibiting pathogenic bacteria adsorption and colonization in human and animal tissues, thereby providing a theoretical basis and an important reference scheme for controlling bacterial infection and biofilm formation.

The application discloses a bocachito Nat10 antibacterial peptide, a coding sequence and use and a preparation method thereof, and relates to a method based on multiomics, a biological big data based on multiomics data of bocachito, screening and identifying an antibacterial peptide precursor gene sequence, and obtaining an amino acid sequence of the bocachito Nat10 antibacterial peptide through translation design, as shown in SEQ ID NO: 3. Through activity simulation prediction and in-vitro experiment verification, the bocachito Nat10 antibacterial peptide shows inhibitory effects on the growth activities of Streptococcus agalactiae, Salmonella enteritidis, Staphylococcus aureus, Vibrio anguillarum and Escherichia coli, especially on Staphylococcus aureus, and shows the best antibacterial effect at a concentration of 2.5 micromoles per liter. The bocachito Nat10 antibacterial peptide can be used for preparing various bactericidal preparations, including bacteriostatic agents, medicines or additives, and has good practical value and social ecological benefits.

The application discloses a gene prdR for regulating myxobacteria predation and a protein coded by the gene, and belongs to the technical field of biotechnology. The mutant DK10 with significantly improved predation capacity is obtained by using the strategy of adaptive evolution; genetic variation sites of the mutant DK10 are analyzed by using the bacterial genome resequencing technology, and functions of the genetic variation sites are researched by using the gene knockout technology, and it is found that the gene deletion of MXAN_2902 (prdR, the nucleotide sequence is shown as SEQ ID NO. 1) significantly enhances the predation capacity of Myxococcus xanthus. The nucleic acid sequence and the amino acid sequence of the transcriptional activator PrdR for regulating the predation of Myxococcus xanthus are disclosed, and the functional strain of Myxococcus xanthus capable of efficiently predating Salmonella enteritidis can be developed by using the prdR gene, and a new strategy is provided for the prevention and control of drug-resistant HVPG pathogenic bacteria.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LS2 and application thereof.The lactobacillus plantarum strain LS2 is separated from cheese, has the high GABA production capacity and the nitrite degradation capacity, has the antibacterial effect on enteric pathogenic bacteria including escherichia coli, salmonella enteritidis and campylobacter jejuni, and has the advantages that the lactobacillus plantarum strain LS2 is high in GABA production capacity and nitrite degradation capacity; the strain has good antibacterial function, good acid resistance and cholate resistance, and can well survive in artificial intestinal juice and artificial gastric juice; in order to improve the yield of GABA, the accelerant capable of remarkably promoting the growth of the strain LS2 is obtained by researching the accelerant, the highest survival rate of the freeze-dried powder prepared by optimizing the accelerant and the freeze-dried powder can reach 99% or above, the nitrite degradation rate can reach 98% or above, the yield of GABA is improved to 3.3 g / L or above, and the freeze-dried powder is bacterial powder capable of being widely applied.

The invention discloses a primer group for simultaneously detecting four equine digestive tract bacteria and application thereof, the primer group comprises primer sequences as shown in SEQ ID NO: 1-8, and the four equine digestive tract bacteria are salmonella enteritidis, salmonella typhimurium, clostridium difficile and lawsonia intracellular. Extracting total DNA (deoxyribonucleic acid) of a sample to be detected by using the excrement sample nucleic acid extraction kit; the total DNA is used as a template, and the primer group is used for multiple PCR reaction to obtain an amplification curve. According to the invention, a primer sequence with high sensitivity and specificity is adopted, so that the quality of a detection result is ensured; the detection method is simple to operate, time-saving and labor-saving; the detection flux is high, and the reagent consumable cost is low.

The invention belongs to the technical field of biology, and discloses a polypeptide with broad-spectrum antibacterial activity and a preparation method and application thereof. The antibacterial peptide or the salt thereof is disclosed for the first time, the antibacterial peptide has a good antibacterial effect on common pathogenic bacteria (such as staphylococcus aureus, escherichia coli, salmonella enteritidis and streptococcus suis) and oral pathogenic bacteria (such as fusobacterium nucleatum and porphyromonas gingivalis), and the antibacterial peptide shows slight cytotoxicity on mammalian cells only under high concentration; and the safety is relatively high.

The invention relates to the technical field of animal medicines, and particularly discloses a veterinary medicine compound preparation as well as a preparation method and application thereof. The veterinary medicine compound preparation is prepared by boiling, extracting, concentrating and diluting eight Tibetan medicines including aconitum pendulum, fructus chebulae, radix inulae inula, benzoin, pterocephalus hookeri, lagotis lagotis, amomum tsao-ko and rhodiola rosea. The veterinary drug compound preparation provided by the invention has an antibacterial range of 8.67 mm to 23.77 mm on enterotoxic large intestine dried bacteria, salmonella enteritidis and pasteurella multocida, and has an inhibition rate of 60% to 72% on enterotoxigenic escherichia coli diarrhea, salmonella enteritidis diarrhea and pasteurella multocida diarrhea; therefore, the veterinary drug compound preparation provided by the invention has a remarkable inhibition effect on enterotoxic large intestine dry bacteria, salmonella enteritidis and pasteurella multocida.

The invention belongs to the technical field of biology, and discloses an antibacterial peptide with activity of inhibiting human pathogenic bacteria as well as a preparation method and application of the antibacterial peptide. The antibacterial peptide or the salt thereof is disclosed for the first time, and the antibacterial peptide has a good antibacterial effect on common genital tract bacteria (such as staphylococcus aureus, gardnerella and candida albicans), oral pathogenic bacteria (such as fusobacterium nucleatum and streptococcus gordoni) and other pathogenic bacteria (such as escherichia coli, salmonella enteritidis and streptococcus suis); and the compound shows slight cytotoxicity to mammalian cells only at a high concentration.

The invention belongs to the field of biology, and discloses application of a detection kit for detecting salmonella enteritidis developed by adopting an SNP (Single Nucleotide Polymorphism) site, the SNP site is the 508th basic group of a Group 25091 gene of the salmonella enteritidis, and the basic group is a T basic group. According to the present invention, the SNP site is adopted as the specific target, such that the high specificity and the high sensitivity are provided, the interference of other serotypes is avoided, and the invention further discloses the primer probe group for detecting the salmonella enteritidis and the related applications.

The invention relates to the technical field of animal medicines, and particularly discloses a veterinary medicine compound preparation as well as a preparation method and application thereof. The veterinary medicine compound preparation is prepared by boiling, extracting, concentrating and diluting eight Tibetan medicines including aconitum pendulum, fructus chebulae, radix inulae inula, benzoin, pterocephalus hookeri, lagotis lagotis, amomum tsao-ko and rhodiola rosea. The veterinary drug compound preparation provided by the invention has an antibacterial range of 8.67 mm to 23.77 mm on enterotoxic large intestine dried bacteria, salmonella enteritidis and pasteurella multocida, and has an inhibition rate of 60% to 72% on enterotoxigenic escherichia coli diarrhea, salmonella enteritidis diarrhea and pasteurella multocida diarrhea; therefore, the veterinary drug compound preparation provided by the invention has a remarkable inhibition effect on enterotoxic large intestine dry bacteria, salmonella enteritidis and pasteurella multocida.

The invention relates to the technical field of poultry immunology, in particular to a universal riemerella anatipestifer monoclonal antibody, a detection kit and application of the universal riemerella anatipestifer monoclonal antibody. Specifically, the invention successfully designs the universal riemerella anatipestifer monoclonal antibody, and the detection kit developed based on the monoclonal antibody can rapidly detect the level of the riemerella anatipestifer serum antibody in a sample, and has high sensitivity and strong specificity. The riemerella anatipestifer strain has no cross reactivity with avian enterococcus faecalis, avian salmonella typhimurium, avian escherichia coli and avian salmonella enteritidis, so that the riemerella anatipestifer strain can be applied to rapid screening, epidemiological monitoring and vaccine immune effect evaluation of riemerella anatipestifer infection, and has a wide application prospect.

The invention discloses a pharmaceutical composition or a vaccine for treating and / or inhibiting salmonella infection, and an application of a small molecule compound Epetrabole hydrochloride. The invention further discloses an application of the pharmaceutical composition or the vaccine and the small molecule compound Epetrabole hydrochloride for treating and / or inhibiting salmonella infection. The MIC value of the small molecule compound is 0.48 [mu] M, and compared with existing antibiotics for clinically treating salmonella enteritidis infection, the small molecule compound has relatively strong antibacterial activity when the small molecule compound is in a trace amount. An in-vitro killing bacteriostatic activity experiment result shows that the small molecule compound has a certain effect on reduction of the number of bacteria. The Salmonella enteritidis strain has obvious antibacterial activity on a J774A.1 cell model, and can inhibit the expression of a salmonella enteritidis virulence factor T3SS1 in a targeted manner. According to the present invention, the small molecule compound Epetrabole hydrochloride provides a certain inhibition effect on salmonella isolates from different sources, and has a wide application value;

The present application relates to the field of microbial technology, and particularly relates to a broad-spectrum lytic Salmonella sp. phage, a composition and application thereof. The phage is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 45281. The phage is isolated from a strain of Salmonella sp., and the core advantage is that (1) it has super-broad-spectrum lytic activity, and can efficiently lyse at least 11 important Salmonella serotypes such as Salmonella enteritidis and Salmonella typhimurium; (2) it has excellent physicochemical stability, and can still maintain high activity after being treated at pH 4-11 and 60 DEG C for 1 hour. Based on the above characteristics, the present application further provides a composition containing the phage, and application thereof in preparing products for food surface disinfection and preservation and prevention and treatment of livestock Salmonella infection. The phage of the present application solves the problems of narrow host spectrum, poor stability and single application scene in the prior art.

The present application provides a kind of pentose lactobacillus YN-02 and product and application prepared from it, belong to biological medicine technical field, the pentose lactobacillus YN-02 is preserved in China typical culture collection on October 22, 2024, and the preservation number is CCTCC NO.M20242300;The gene sequence of 16srRNA of the pentose lactobacillus YN-02 is as shown in SEQ ID NO.1.The present application uses the above-mentioned pentose lactobacillus YN-02 and product and application prepared from it, has significant effect in anti-listeria monocytogenes, salmonella enteritidis, escherichia coli, staphylococcus aureus, salmonella typhimurium infection.

The invention establishes a method for simultaneously detecting vibrio parahaemolyticus, staphylococcus aureus, listeria monocytogenes, salmonella, shigella, bacillus cereus and vibrio alginolyticus on the basis of multiple PCR (Polymerase Chain Reaction) combined with a gene chip technology. The method comprises the following steps: selecting a heat-labile hemolysin gene of vibrio parahaemolyticus, a heat-resistant nuclease gene of staphylococcus aureus, a coded hemolysin O gene hlyA of listeria monocytogenes, an invasive protein A gene of salmonella enteritidis and an invasive plasmid antigen H gene of Shigella to carry out quintuple PCR (Polymerase Chain Reaction) amplification; bacillus cereus non-hemolytic toxin genes and vibrio alginolyticus similar Rpos sigma factor genes are selected for double PCR, two groups of multiplex PCR amplification products are hybridized with specific probes on the chip at the same time, and seven food-borne pathogenic bacteria can be detected at the same time. The method for detecting pathogenic bacteria by using the gene chip is high in specificity, high in sensitivity and strong in reliability.

The application belongs to the field of biology, and discloses a use of a SNP site for developing a detection kit for detecting Salmonella enteritidis, the SNP site being a 508th base of a group_25091 gene of the Salmonella enteritidis, and the base being a T base. The application uses the SNP site as a specific target, has higher specificity and sensitivity, and avoids interference of other serotypes. Meanwhile, the application also discloses a primer probe group for detecting the Salmonella enteritidis and related applications.

The invention belongs to the technical field of biology, and discloses an antibacterial peptide for regulating and controlling micro-ecology as well as a preparation method and application of the antibacterial peptide. The antibacterial peptide or the salt thereof is disclosed for the first time, and the antibacterial peptide has a good antibacterial effect on common oral pathogenic bacteria (such as fusobacterium nucleatum and streptococcus gordoni), genital tract pathogenic bacteria (such as staphylococcus aureus, gardnebacterium and enterococcus faecalis) and other pathogenic bacteria (such as escherichia coli, salmonella enteritidis and streptococcus suis); and the compound shows slight cytotoxicity to mammalian cells only at a high concentration.

The application provides a feed additive suitable for Danzhou chicken and a preparation method thereof, which comprises a Callicarpa nudiflora complex, a wine lees fermentation extract and an eleocharis tuberose peel; the wine lees fermentation extract is obtained by fermenting wheat flour and egg white powder with sweet wine lees, and then performing enzymatic dissolution with protease and glucanase, and then concentrating and drying; the feed additive suitable for Danzhou chicken is prepared from the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel; the feeding test proves that the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel have a good promoting effect on the production performance of Danzhou chicken, effectively improve the feed conversion rate, and reduce the mortality rate; and the inhibition rates of the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel on six kinds of bacteria, including Salmonella enteritidis, Salmonella typhi, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Proteus mirabilis, are all above 90%.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LT400 and application thereof.The lactobacillus plantarum strain LT400 is separated from acid materials, can remarkably improve the aroma of sour mustard and increase the content of flavor substances, has a bacteriostatic effect on Escherichia coli, and can be used for preparing sour mustard. A fermentation product obtained by fermenting the strain and leaf mustard has an antibacterial effect on salmonella enteritidis, escherichia coli and staphylococcus aureus; the bacterial strain LT400 cooperates with alkaloids: mulberry alkaloid, passion fruit alkaloid and / or rosa roxburghii tratt fruit alkaloid, so that the brightness of the pickled Chinese cabbages can be effectively improved, and the pickled Chinese cabbages with better color and luster can be prepared; experiments prove that the alkaloid comes from fruits, the product source is green and healthy, the alkaloid is a healthy and effective pickled Chinese cabbage brightness improving agent, the alkaloid and the strain LT400 cooperate to provide a new thought for subsequent development of an anti-browning agent, the fermentation liquor has the value of researching and developing antibacterial peptides, and a new technical concept is provided for later improvement of the added value of the product.

The present invention discloses a monoclonal antibody CJ116 against pathogenic Campylobacter and its application. In the monoclonal antibody CJ116, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID NO.1, and the amino acid sequence of the light chain variable region is as shown in SEQ ID NO.5. This monoclonal antibody CJ116 has high affinity and high specificity, and the detection sensitivity for Campylobacter jejuni and Campylobacter coli can reach 10<supgt;2< / supgt> CFU / mL, and the detection sensitivity for Campylobacter fetus, Campylobacter upsaliensis and Campylobacter lari is 10<supgt;3< / supgt> CFU / mL, but there is no cross-reaction with other common intestinal pathogenic bacteria such as pathogenic Escherichia coli, Salmonella enteritidis, Yersinia enterocolitica, Shigella flexneri, Shigella boydii and Staphylococcus aureus.

This invention relates to the field of avian immunology, and more particularly to a universal monoclonal antibody against Riemerella anatipestifer, a detection kit, and their applications. Specifically, this invention successfully designed a universal monoclonal antibody against Riemerella anatipestifer, and the detection kit developed based on this monoclonal antibody can rapidly detect the level of Riemerella anatipestifer serum antibodies in samples with high sensitivity and strong specificity. Furthermore, it shows no cross-reactivity with Enterococcus faecalis, Salmonella typhi, Escherichia coli, and Salmonella enteritidis, thus it can be used for rapid screening, epidemiological monitoring, and evaluation of vaccine immunization efficacy against Riemerella anatipestifer infection, with broad application prospects.

The invention relates to the technical field of salmonella bacteriophages, and in particular relates to a bacteriophage capable of splitting salmonella brassicae as well as a composition and application of the bacteriophage capable of splitting the salmonella brassicae. The bacteriophage is salmonella bacteriophage RDP-SA-22010, the preservation number of the bacteriophage is CGMCC (China General Microbiological Culture Collection Center) No.45274, and the bacteriophage is preserved in the China General Microbiological Culture Collection Center on September 21, 2022. The salmonella bacteriophage RDP-SA-22010 can also be used for cracking salmonella enteritidis, salmonella pullorum and salmonella kafenkii. The invention also relates to a composition, which comprises the salmonella bacteriophage RDP-SA-22010. The reagent or the kit comprises the composition. The invention also provides application of the salmonella bacteriophage RDP-SA-22010 to preparation of a biological bactericide, preparation of a medicine for treating diseases caused by salmonella and preparation of a feed additive. The salmonella bacteriophage RDP-SA-22010 disclosed by the invention provides a novel biological prevention and treatment means for the prevention and treatment of salmonella bulidan.

The present application relates to the technical field of microorganism, and especially relates to a kind of Enterococcus durans C24, probiotic agent and application thereof.The present application provides Enterococcus durans C24, and its preservation number is CGMCC No.33995.The strain has broad-spectrum antibacterial activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, and has good stress resistance, can survive in gastrointestinal environment, and also has high biological safety, and is expected to be used for clinical prevention and control of salmonellosis.

The application discloses a Salmonella multi-epitope protein, a coding gene and application of the Salmonella multi-epitope protein in preparation of a Salmonella subunit vaccine, and relates to the field of biotechnology.The amino acid sequence of the Salmonella multi-epitope protein is shown as SEQ ID NO.2.The nucleotide sequence of the coding gene of the Salmonella multi-epitope protein is shown as SEQ ID NO.1.The Salmonella subunit vaccine prepared by using the Salmonella multi-epitope protein has the advantages of safety and stability, low cost, no toxicity to the body, and the ability to simultaneously cause the body to produce humoral immunity and cellular immunity, and the like, and can not only produce a preventive protection effect on Salmonella enteritidis infection, but also can play a cross protection effect on Salmonella typhimurium infection.

The invention belongs to the technical field of microbial sterilization preparations, and particularly discloses a broad-spectrum salmonella bacteriophage SD40-16 and application of the broad-spectrum salmonella bacteriophage SD40-16. The bacteriophage SD40-16 can be used for cracking six serotypes of salmonella and escherichia coli including salmonella enteritidis and salmonella typhimurium, is wide in host range and strong in killing capability, and can be used for inhibiting the growth of bacteria. And meanwhile, the strain has good thermal stability and acid-base tolerance, and is easy to proliferate and enrich. The bacteriophage can enhance the antibacterial ability of kanamycin, provides a new drug combination strategy, has an obvious synergistic effect on salmonella resistance when being combined with kanamycin, and has a good application prospect in the aspect of preventing and treating salmonella infection.

The invention discloses lactobacillus johnsonii for preventing and treating pullorum disease salmonellosis and application of the lactobacillus johnsonii, and belongs to the field of microorganisms. According to the invention, a strain of Lactobacillus johnsonii LMZ8 is separated and screened, and the preservation number of the strain of Lactobacillus johnsonii LMZ8 is GDMCC No: 66614. The lactobacillus johnsonii LMZ8 is good in acid resistance and has certain tolerance to high-concentration cholate. Experimental results show that the lactobacillus johnsonii LMZ8 can effectively inhibit the growth of various pathogenic bacteria such as salmonella typhimurium and salmonella enteritidis, especially has a prominent inhibition effect on five strains of multi-drug-resistant salmonella pullorum with no same drug resistance spectrum, can effectively prevent and treat the pullorum disease caused by salmonella pullorum, and improves the growth performance of chicks. The lactobacillus johnsonii LMZ8 shows remarkable development potential in the aspects of preventing and controlling food-borne pathogenic bacteria and preventing and treating diseases such as pullorum disease, and has a wide development prospect.