223 results about "Enterobacterales" patented technology

The invention provides a Lactobacillus paracasei, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.32951. The Lactobacillus paracasei has a preservation number of CGMCC No.32951. The Lactobacillus paracasei has a preservation number of CGMCC No.32951. The lactobacillus paracasei ID001 provided by the invention has an inhibition effect on enteropathogenic bacteria such as salmonella, escherichia coli, staphylococcus aureus and the like. The probiotic preparation is obtained through fermentation and the dendrobium officinale extract, and the probiotic preparation has the immunoregulation effect through performance verification and screening.

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The invention discloses construction and application of an escherichia coli L-tryptophan biosensor, and belongs to the technical field of bioengineering. The L-tryptophan biosensor provided by the invention comprises a mutated leader peptide fragment tnaC-tnaA, a promoter of the mutated leader peptide fragment tnaC-tnaA, a green fluorescent protein coding gene eGFP and a pTrc99a plasmid skeleton, a recombinant strain containing the L-tryptophan biosensor is cultured, the expression of green fluorescent protein in a culture solution is gradually enhanced along with the increase of the concentration of the L-tryptophan, and the fluorescence value of a unit cell shows better correlation when the concentration of the L-tryptophan is 0-1.5 g / L; therefore, when the biosensor is used for detecting the L-tryptophan in the fermentation liquor, the biosensor has the characteristics of simplicity in operation, sensitive response and high threshold value.

The invention discloses a bacillus strain H7 and an application thereof. The invention provides bacillus velezensis H7 or a progeny of the bacillus velezensis H7, and the preservation number of the bacillus velezensis H7 in the China Center for Type Culture Collection (CCTCC) is CCTCC NO: M 2025630. The bacillus velezensis H7 disclosed by the invention has a very good antibacterial effect on various common clinical pathogenic bacteria (including klebsiella pneumoniae, escherichia coli, candida albicans, enterobacter cloacae complex, acinetobacter baumannii and the like), and particularly shows a very high inhibition rate on some drug-resistant clinical pathogenic bacteria; therefore, the compound has important application value in development of drug and non-drug antibacterial products (such as medical antibacterial materials and the like), a new choice can be provided for drug and non-drug prevention and control of clinical pathogenic bacterium infection, and particularly, the compound has a supplementary effect in the aspects of reducing drug-resistant bacterium propagation risk and reducing drug dependence.

The invention relates to a method for increasing the yield of L-malic acid produced by fermentation by adding ethylene glycol, which comprises the following steps: constructing genetic engineering escherichia coli, and introducing ethylene glycol metabolism genes (overexpression) including glycerol dehydrogenase, lactaldehyde reductase, lactaldehyde dehydrogenase and the like; and overexpression of other genes involving malic acid production, such as malic acid synthase, phosphoenolpyruvate carboxylase and malic acid export protein. The gene engineering strain is an E.coli BW25113 strain of which ldhA, pta, poxB, adhE, pflB, mgsA, frdA, maeA, maeB, yqhD, ycdW and iclR genes are substantially inactivated, and the deleted genes are beneficial to reducing the generation of metabolic byproducts and increasing the accumulation of malic acid. The genetic engineering strain is subjected to fermentation culture by taking glucose and ethylene glycol as carbon sources, and L-malic acid is separated from a culture after culture is finished.

The present invention discloses a broad-spectrum antibacterial peptide, and the amino acid sequence of the antibacterial peptide is GECEGNNRPVKKPQPRKKLPRFKKC-NH2. Based on melittin, the present invention designs antibacterial peptides by predicting based on a deep learning model, and obtains antibacterial peptides with broad-spectrum bacteriostasis, thermal stability and low hemolytic property, which have good inhibitory effects on skin pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Propionibacterium acnes. Peptides predicted as AMP amino acid sequences by the deep learning model antibacterial peptide prediction tool amPEPpy are selected for screening, and by using a biosynthesis technique, they are expressed, extracted and purified in Escherichia coli, reducing the workload and screening period of screening, and increasing the probability of screening out broad-spectrum and thermally stable biosynthetic antibacterial peptides.

The invention discloses a saline-alkaline tolerant compound microbial agent and application thereof, and particularly relates to the technical field of microbial soil treatment. The microbial agent comprises Klebsiella, Enterobacter hormaechei and humic acid, the ratio of the components is as follows: Klebsiella: Enterobacter hormaechei: humic acid = 1: (0.5-2): (1-3), and both Klebsiella and Enterobacter hormaechei are obtained by fermentation culture. According to the present invention, the specific pH gradient is adopted for the Klebsiella and the Enterobacter hormaechei, and the segmented fermentation process combining the specific pH gradient with the corresponding gradually-increased sodium chloride concentration is adopted, such that the strain is effectively subjected to the directional domestication so as to maintain the activity and the function in the high-salt and high-alkali extreme environment.

The invention discloses a urinary system infection pathogen and drug resistance gene detection system and a kit. The detection system comprises forward and reverse primers which are respectively used for detecting escherichia coli, staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, enterococcus faecium, candida glabrata, proteus mirabilis, enterococcus faecalis, candida tropicalis, enterobacter cloacae, candida albicans, pseudomonas aeruginosa and streptococcus agalactiae; the forward primer and the reverse primer are respectively used for detecting the blaCTX-M gene of the extended spectrum beta-lactamase, the forward primer and the reverse primer are used for detecting the blaSHV gene of the extended spectrum beta-lactamase, and the forward primer and the reverse primer are used for detecting the blaTEM gene of the extended spectrum beta-lactamase. According to the forward and reverse primers for detecting the blaNDM gene of the extended-spectrum beta-lactamase, a detection sample is urine. According to the invention, common gram-negative bacteria, gram-positive bacteria and fungi can be detected at the same time, and the problem of missed detection of mixed infection in traditional culture is solved.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Mh-GC21 as well as a precursor and application thereof. The amino acid sequence of the antibacterial peptide Mh-GC21 provided by the invention is as shown in SEQ ID NO.1, and two cysteines are oxidized to form a pair of intermolecular disulfide bonds; the C tail end of the antibacterial peptide Mh-GC21 is subjected to amidation modification. The antibacterial peptide Mh-GC21 provided by the invention is from microhyla microcarpa, has broad-spectrum antibacterial activity, has relatively good antibacterial and bactericidal effects on standard strains of acinetobacter baumannii, escherichia coli and staphylococcus aureus and clinically sourced drug-resistant strains, and can be used for targeted destruction of cell membranes of bacteria, removal of biological membranes and inhibition of formation of the biological membranes. Moreover, the antibacterial peptide Mh-GC21 has the advantages of good stability, no hemolytic activity and cytotoxicity, and difficulty in inducing strains to generate drug resistance.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

The invention provides a method for synthesizing D-psicose through fermentation of escherichia coli by using D-glucose and D-xylose. The method comprises the following steps: by taking escherichia coli as a host cell, over-expressing a galactose permease gene galP, a glucokinase gene glk, a 6-phosphate glucose isomerase gene pgi, a D-psicose-6-phosphate epimerase gene alsE with an SUMO tag and a D-psicose-6-phosphate phosphatase gene a6PP, and constructing a path for synthesizing D-psicose by taking D-glucose and D-xylose as substrates; the synthetic route of D-psicose is optimized by knocking out a D-glucose phosphorylation transport pathway gene ptsG, knocking out D-fructose-6-phosphokinase genes pfkA and pfkB, silencing a glucose-6-phosphate dehydrogenase gene zwf, overexpressing glutamate transhydrogenase genes gdh1 and gdh2, knocking out a UDP-glucose-4-epimerase gene galE and knocking out a transport protein key gene fryA in D-psicose; and finally, the optimal proportion of mixed sugar fermentation is explored, so that efficient synthesis of D-psicose from escherichia coli through mixed sugar fermentation of D-glucose and D-xylose is realized.

The invention discloses an application of NSC348884 in preparation of an antibacterial drug, the bacteria of the NSC348884 are gram-negative bacteria or drug-resistant gram-negative bacteria, and the gram-negative bacteria are acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa or klebsiella pneumoniae; based on an antibacterial drug high-throughput screening technology, it is found from 1280 small molecule compounds that the benzimidazole compound NSC348884 has direct bacteriostasis and sterilization effects on gram-negative bacteria and drug-resistant gram-negative bacteria, and no drug resistance is generated after the benzimidazole compound NSC348884 is continuously applied for 14 days. Further research shows that NSC348884 can interfere with lipid synthesis of gram-negative bacterium cell membranes, inhibit formation of biological membranes and induce lipid metabolism disorder, so that bacteriostatic and bactericidal effects are achieved. In addition, the NSC348884 can also enhance the sensitivity of the drug-resistant bacteria to the imipenem and the polymyxin B.

The invention provides a fluorenyl alcohol derivative which can be used as an antibiotic adjuvant with a broad-spectrum synergistic effect. According to the compound, bacteria outer membrane asymmetric protein MlaC and phospholipase A1 PldA are taken as targets, and a series of fluorenyl alcohol derivatives targeting the bacteria outer membrane asymmetric protein MlaC and phospholipase A1 PldA are obtained through screening. The preferable compound WTU-15 can improve the antibacterial activity of antibiotics such as polymyxin, cefepime, tetracycline and the like on negative bacteria such as sensitive and drug-resistant escherichia coli, salmonella, klebsiella pneumoniae, acinetobacter baumannii and the like, and can also improve the in-vivo treatment effect of cefepime on mice infected by the drug-resistant acinetobacter baumannii. And the visceral organs of the mice survived after administration have fewer bacteria, and the mice are better after being healed. Normal physiological and visceral organ functions of the mouse are not affected by single and continuous administration of the WTU-15.

The invention relates to the technical field of PA resin, and particularly discloses glass fiber modified antibacterial PA resin suitable for children products and a preparation method of the antibacterial PA resin. The invention discloses glass fiber modified antibacterial PA resin suitable for children products. The glass fiber modified antibacterial PA resin comprises PA resin, glass fibers, talcum powder, nano calcium carbonate, a composite antibacterial agent, a compatilizer, an antioxidant and calcium stearate, the composite antibacterial agent comprises chitosan, an antibacterial peptide microcapsule and a porous silver-zinc-loaded antibacterial agent; the antibacterial peptide microcapsules are sodium alginate embedded antibacterial peptides. The antibacterial PA resin obtained in the invention has the highest bacteriostasis rates of 99.97%, 99.74% and 99.95% for staphylococcus aureus, escherichia coli and candida albicans respectively, the antibacterial property of the PA resin is improved, the compressive strength and notch impact strength are 170.6 MPa and 17.3 kJ / m < 2 > respectively, and high mechanical strength is maintained on the basis of high antibacterial property.

The invention discloses a larimichthys crocea IL-22 recombinant protein which is a larimichthys crocea IL-22 mature peptide sequence, and the amino acid sequence of the larimichthys crocea IL-22 recombinant protein is as shown in SEQ ID NO. 2. The recombinant protein is obtained by amplifying and coding gene segments of amino acids from 31st to 190th positions of large yellow croaker IL-22 mature peptide through PCR (Polymerase Chain Reaction), and transforming escherichia coli for heterologous expression. The larimichthys crocea IL-22 recombinant protein can activate a transcription factor STAT3 in a larimichthys crocea head kidney cell line (LYCK) and also can induce expression of inflammatory factors (IL-1beta, IL-6 and IL-8) in immune organs of larimichthys crocea, so that the larimichthys crocea IL-22 recombinant protein has an obvious immunoregulation effect and shows a good application prospect in research and development of aquatic immunomodulators.

The invention belongs to the technical field of microorganisms, and particularly discloses lactobacillus plantarum CX1-3-2 capable of producing gamma-aminobutyric acid and improving sleep and application of the lactobacillus plantarum CX1-3-2. The preservation number of the lactobacillus plantarum CX1-3-2 strain is CGMCC (China General Microbiological Culture Collection Center) No. 34340. The lactobacillus plantarum CX1-3-2 can achieve the effect of improving sleep by improving the levels of GABA and 5-HT in brain tissues and reducing the levels of glutamic acid Glu, dopamine DA and noradrenaline NE; the scavenging rate of DPPH free radicals of the strain reaches 96.4%, the scavenging rate of ABTS free radicals of the strain reaches 78.3%, and the strain has an excellent anti-oxidation effect; the diameters of inhibition zones of the strain on staphylococcus aureus, bacillus cereus, escherichia coli and pseudomonas fluorescens are all larger than 17 mm, and the strain has an excellent antibacterial effect.

The invention discloses a tannic acid-silver palladium (TA-Ag / Pd) nano material and a preparation method and application thereof, and belongs to the technical field of biological materials.The method comprises the steps that Na2PdCl4 is added into water containing HCl, and a palladium solution is obtained; the palladium solution and the silver nitrate solution are added into the tannic acid solution for vortex mixing, and a mixed solution is obtained; and carrying out constant-temperature treatment on the mixed solution, washing, centrifuging and drying to obtain the TA-Ag / Pd nano material. The preparation method provided by the invention is mild in reaction condition, simple and convenient in preparation process, short in time and high in efficiency, and the prepared TA-Ag / Pd nano-material shows a remarkable antibacterial effect, can effectively inhibit growth of bacteria such as escherichia coli and staphylococcus aureus, is suitable for the fields of medical instruments, food packaging, environmental protection and the like, and has wide application prospects. The method has remarkable application advantages and wide market potential.

The invention relates to the technical field of microbiological detection, and particularly discloses a primer probe combination for detecting six food-borne pathogens and application of the primer probe combination. The detection primer probe combination for the six food-borne pathogens comprises six pairs of specific primer pairs and six probes, the nucleotide sequences of the specific primer pairs are respectively shown as SEQ ID NO.1, SEQ ID NO.4, SEQ ID NO.8, SEQ ID NO.1, SEQ ID NO.13, SEQ ID NO.16, SEQ ID NO.19, SEQ ID NO.22, SEQ ID NO.26, SEQ ID NO.29, SEQ ID NO.32 and SEQ ID NO.35, and the nucleotide sequences of the probes are respectively shown as SEQ ID NO.37-SEQ ID NO.42; the six food-borne pathogens comprise listeria monocytogenes, salmonella, vibrio parahaemolyticus, norovirus, escherichia coli O157: H7 and staphylococcus aureus. According to the present invention, the pathogens of six common food-borne diseases can be detected, the detection method has characteristics of high specificity and high sensitivity, and the pathogens do not interfere with each other and do not inhibit each other during the detection so as to achieve good stability.

The invention provides a red light carbon quantum dot as well as a preparation method and application thereof. The red light carbon quantum dot is prepared from fulvic acid and polyamine as raw materials through a hydrothermal reaction. The fulvic acid is used as a carbon source, the polyamine is used as a nitrogen source, the carbon quantum dots are prepared through a hydrothermal reaction, the obtained carbon quantum dots are small in size, and the particle size is 1-3 nm; the red light emission wavelengths are 680 nm and 720 nm, and the photoluminescence characteristic is irrelevant to the excitation wavelength; the prepared quantum dot has good biocompatibility, the quantum efficiency is greater than or equal to 20%, the prepared quantum dot has good bacteriostatic and antibacterial activity, and the inhibition rate on staphylococcus aureus, pseudomonas aeruginosa, enterobacter cloacae, escherichia coli, klebsiella pneumoniae or methicillin-resistant staphylococcus aureus under the concentration of 2 mu g / mL can reach 99.9%. Wide application prospects are realized in the aspects of antisepsis, anti-inflammation and wound healing promotion.

The present invention relates to the pharmaceutical field, and provides a boric acid β-lactamase inhibitor or a use thereof. The boric acid β-lactamase inhibitor is a compound shown in formula (I), or an optical isomer or a pharmaceutically acceptable salt thereof: formula (I). The ultra-broad-spectrum boric acid β-lactamase inhibitor of the present invention has an inhibition effect on A, B, C, and D enzymes, and carbapenem drug-resistant bacteria such as Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa.

The invention relates to the technical field of bioengineering, in particular to a penicillin G acylase mutant, polynucleotide, an expression vector and application. The penicillin G acylase mutant is obtained by carrying out site-directed mutagenesis on a wild type penicillin G acylase gene of parent Escherichia coli, and carrying out site-directed mutagenesis on the wild type penicillin G acylase gene to obtain the penicillin G acylase mutant. Phenylalanine (F) at the 24th site of an alpha chain, proline (P) at the 383rd site of a beta chain, threonine (T) at the 384th site of the beta chain, glutamic acid (G) at the 385th site of the beta chain and serine (S) at the 386th site of the beta chain of the penicillin G acylase are introduced and mutated by a whole plasmid PCR (Polymerase Chain Reaction) technology to obtain mutants. When the penicillin G acylase mutant obtained by the invention is used for catalyzing methyl mandelate and 3-(1-methyl-1H-tetrazole-5-yl) thiomethyl-7-aminocephalosporanic acid to synthesize cefamandole, the synthesis activity is improved, and meanwhile, the side reaction rate is greatly reduced.

The invention provides a primer group, a kit and a method for simultaneously detecting 11 microorganisms and diagnosing chronic endometritis intrauterine infection. The primer group is used for detecting the existence of 11 chronic endometritis pathogens: chlamydia trachomatis, enterococcus faecalis, escherichia coli, trichomonas vaginalis, klebsiella pneumoniae, mycoplasma hominis, neisseria gonorrhoeae, staphylococcus, streptococcus, reproductive mycoplasma and ureaplasma urealyticum. The primer group is used for performing multiple joint detection on chronic endometritis related pathogens, has high specificity and sensitivity, and is adaptive to an endometrial tissue sample. The invention provides a method for simultaneously detecting 11 microorganisms for diagnosing chronic endometritis intrauterine infection, which comprises the following steps of: collecting an endometrial sample by adopting a disposable sterile endometrial sampler, detecting 11 microorganisms in the endometrial sample by adopting a multiplex PCR (Polymerase Chain Reaction) technology, and accurately detecting the intrauterine microbial infection condition. Meanwhile, more accurate technical support is provided for clinically identifying whether pathogenic bacteria causing chronic endometritis exist or not, and clinical doctors can comprehensively consider medication guidance conveniently.

This invention relates to a method for producing chondroitin with improved safety and extracellular secretion levels, belonging to the field of genetic engineering. Using the probiotic *Escherichia coli* Nissle 1917 as the host, this invention designs methods to increase chondroitin production by studying the chondroitin synthase KfoAC and the chondroitin-linking Kdo synthase. The relationship between chondroitin synthesis and secretion in *E. coli* was clarified, and the specificity of the chondroitin-linking Kdo was investigated, resulting in an extracellular chondroitin production of 0.32 g / L. Subsequently, the introduction of the protein KfoB from the chondroitin synthesis gene cluster increased the extracellular chondroitin production to 0.51 g / L. Further screening and optimization of the expression of transport proteins resulted in an extracellular chondroitin production of 0.73 g / L in shake flasks. Further optimization using a 7-L fermenter resulted in an extracellular chondroitin production of 5.9 g / L.

The present invention relates to the use of a combination of a saccharide and glycerol for skin protection against undesirable bacteria. The present invention is particularly useful in the formulation of compositions that act as prebiotics for commensal skin bacteria such as S. epidermidis to produce metabolites such as lactic acid, which, through the present invention, has been shown to inhibit the growth of undesirable bacteria such as E. coli and S. aureus, among others. The present invention is especially useful because it provides both short-term (i.e., rapid) and long-term (or sustained) protection.

The invention provides a plant fiber containing a ginger extract and a preparation process of the plant fiber. After the plant fiber is washed for 50 times, the bacteriostasis rates of the plant fiber to staphylococcus aureus, escherichia coli, klebsiella pneumoniae and candida albicans are all greater than or equal to 95%, the killing rates of the plant fiber to influenza viruses H3N2 and H1N1 are all greater than or equal to 99%, the far infrared emissivity is 0.89-0.95, the highest temperature rise of moisture absorption and heating is 4-12 DEG C, and the average temperature rise is 3-8 DEG C. A vapor deposition method is adopted, a biomass component A and a biomass component B are combined to form a biomass component C, on one hand, the biomass component B serves as a tube bundle-shaped base material, rhizome plants are calcined to form a porous carbon structure framework, the porous carbon structure framework serves as a solid for vapor deposition, and a solution containing the biomass component A is small in gas molecular weight; after being vaporized along with the solution, the solution is caught by a porous structure of the biomass component B and uniformly filled into pores of the biomass component B, so that the durability of plant functions is ensured, and meanwhile, a protection effect at high temperature is achieved.

The invention belongs to the technical field of microbiological detection, and particularly relates to a rapid escherichia coli test box which comprises a detection box, a sterile box, a multi-stage feeding device and a first motor, the first motor controls the working stroke of the multi-stage feeding device, and then the dosage of sterile water added into the sterile box is controlled; the farther the first motor is, the more sterile liquid is added into the sterile box, the smaller the dilution degree is, and the closer the first motor is, the less sterile liquid is added into the sterile box, and the larger the dilution degree is, so that the three sterile boxes are sequentially placed, and three diluents with different dilution degrees are obtained; the method does not need to manually separately use a sterilization suction tube to suck sterile water of different doses and add the sterile water into diluents of different diluents to obtain the diluents of different diluents, so that the detection time is greatly saved, and the detection efficiency is improved.

The invention relates to a bacteriophage, in particular to a cross-host multivalent bacteriophage and preparation and application of a bacteriostatic agent of the bacteriophage, the multivalent bacteriophage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026045. The salmonella bacteriophage CCTCC NO: M 2026045 is found for the first time, is a multivalent bacteriophage, can cross-host infect and split multiple important livestock and poultry intestinal pathogens such as salmonella, escherichia coli, shigella, klebsiella pneumoniae and proteus mirabilis, solves the problem of narrow host range of a single bacteriophage, and improves the prevention and control efficiency. By inhibiting the overgrowth of the gram-negative pathogenic bacteria, the intestinal microecological balance can be regulated, so that the aim of effective prevention and control is fulfilled.

The invention relates to the technical field of high polymer material processing, in particular to an antimicrobial high polymer material product and a preparation method thereof.The antimicrobial high polymer material product is obtained by applying photon broadband antibacterial treatment to the high polymer material product; the high polymer material comprises plastic or rubber, the plastic comprises polyethylene or polyvinyl chloride, and the rubber comprises butyronitrile; the high polymer material product is an elastomer product; the elastomeric articles include glove fingerstalls, dams, probe sleeves, or medical catheters. The antimicrobial high polymer material product has an excellent inhibition effect on staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, candida albicans and the like, the antibacterial rate can reach 89% or above, the preparation process is simple, the cost is controllable, and the antimicrobial high polymer material product is suitable for industrial production.

The invention relates to the technical field of organic chemistry, and particularly discloses an amide derivative containing a thiazole oxime structure as shown in a general formula 8 and a preparation method and application of the amide derivative. The derivative shows remarkable antibacterial activity on staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and fluoroquinolone-resistant escherichia coli, particularly has a remarkable effect on gram-negative bacteria, has broad-spectrum antibacterial potential and can be used for preparing drugs for treating related bacterial infection, and the activity of part of compounds is superior to that of a control drug.

The present invention provides a method for producing 2-methyl-butyric acid by fermentation using a bacterium belonging to the order Enterobacterales which has been modified to attenuate expression of a tyrB gene encoding a protein having tyrosine aminotransferase activity. The method also allows for production of a byproduct substance of 2-methyl-butyric acid during fermentation of the Enterobacterales bacterium having 2-methyl-butyric acid-producing ability.