Niacinamide ribose phosphate transferase for preparing NMN, coding gene, recombinant vector and application

Abstract

The invention discloses a niacinamide ribose phosphate transferase for preparing NMN, a coding gene, a recombinant vector and application and belongs to the technical field of biology. The transferaseis protein of which the amino acid sequence is shown in pSEQ ID No.1 or pSEQ ID No.2 or pSEQ ID No.3 or pSEQ ID No4. The gene for coding the protein is cloned to an expression vector of escherichia coli, and a recombinant plasmid is established; then, the recombinant plasmid is converted into escherichia coli competent cells, a positive clone is selected, an expression strain of recombinant escherichia coli is obtained, and then liquid fermentation is conducted; in the fermentation process, the generated recombinant niacinamide ribose phosphate transferase is generated, and after a substrateis added, beta-nicotinamide mononucleotide can be generated. According to the transferase, NMN is conveniently and efficiently prepared, the fermentation technology is simple, the consumed time is short, the cost is low, the cost of obtaining NMN is 0.25-1.0 yuan/mg, compared with a traditional method, the cost can be lowered by above 90%, the yield reaches 51.75 mg/g of protein, and the economicbenefit is obvious.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for easy production of beta - nicotinic acid ammonia salt or its precursor ribitolimidate from natural sources like bacterial strains that produce it through enzymatic reactions called nitrogenase activity. These processes involve converting certain chemicals into different forms such as α-, γ-, or lysosomal proteases which help break down these molecules into smaller compounds containing other substances needed during metabolism. By utilizing this technique, researchers have been able to make new drugs targeted at specific diseased areas without causing side effectes on healthy tissues.

Problems solved by technology

This patented technical solution describes how nerve cells can produce nitric acid without being affected by other factors like nutrients or oxygen levels during their growth process.

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