188results about How to "High protection rate" patented technology

The invention provides a method and a device of realizing a backup route. The method comprises the steps that a first node uses the Remote LFA technology to calculate a PQ node after calculating a main route from the current node to a second node; if the PQ node is calculated, the first node adds a route table entry corresponding to the backup route in a route forwarding table, wherein the route table entry comprises a node segment label of the second node, an outlet port and a corresponding label stack, wherein the outlet port is a port used for connecting a backup next hop node on the current node, and the label stack comprises a node segment label of the PQ node and the node segment label of the second node; and when it is detected that a link between the current node and a main next hop node is disconnected, the first node compresses the label stack in the route table entry in a user message to be sent to the second node according to the route table entry corresponding to the backup route to acquire a package message, and forwards the package message through the outlet port in the route table entry.

The invention discloses an egg yolk antibody for preventing novel goose astrovirus and a preparation method thereof. The egg yolk antibody contains a goose astrovirus strain resistance antibody; the preservation number of the goose astrovirus strain is CCTCC NO:V201808. The prepared egg yolk antibody is good in safety, and no any partial or whole adverse reaction is caused by the egg yolk antibody; infections caused by novel goose astrovirus can be effectively prevented and/or treated, and the good commercialization development prospect is achieved.

The invention relates to a disinfectant for a pond water body, comprising the following components in parts by weight: 10-100 parts of potassium ferrate, 100-300 parts of polymeric aluminium, 200-500 parts of polymeric ferric sulphate and 100-700 parts of clinoptilolite. Ferrate used in the invention is taken as a non-chlorine water treatment agent, bacteria, viruses and algaes in water can be rapidly killed, organic matters, inorganic matters and heavy metal ions in the water also can be removed, and the effects of disinfection, decolourization, deodorization and coagulation assistant can be realized. The disinfectant for the pond water body disclosed by the invention has reasonable components and low cost, is simple to prepare and is convenient to use, development and application curative effects are assured, toxic and side effects are small, and application range is wide.

The invention discloses recombinant porcine reproductive and respiratory syndrome virus (PRRSV) virus-like particles (VLP) and a preparation method and an application thereof. Based on comparative analysis of GP5 of a PRRSV epidemic strain and an M gene sequence, a GP5 and M tandem sequence GP5M is synthesized artificially, the synthesized GP5M gene sequence is cloned into a vector with a pBAC5 plasmid as a skeleton, the baculovirus transfer vector pBAC-PRRSVGP5M is obtained, the recombinant bacmid rBacmid-GP5M is obtained, sf9 cells are transfected with the bacmid, and the recombinant baculovirus Ac-PRRSVGP5M is obtained. The PRRSV GP5 and M protein are expressed efficiently by the recombinant baculovirus, and the virus-like particles are formed. A subunit vaccine prepared by the proteinexpressed by the recombinant baculovirus can induce a body to produce a specific immune response after immunizing animals and can protect the pig body against the strong poison attacking of porcine reproductive and respiratory syndrome virus.

The invention aims to provide an egg yolk antibody for preventing and curing muscovy duck parvovirus diseases, and the egg yolk antibody is prepared by using muscovy duck parvoviruses the preserving number of which is CGMCC (China General Microbiological Culture Collection Center) No.8504 as an antigen. The egg yolk antibody is characterized in that a muscovy duck parvoviruse YBMDP plant is inoculated with a muscovy duck embryo, a die embryo body and embryo liquid are respectively obtained and are grinded and are subjected to freeze thawing so as to obtain a virus solution, and the oil adjuvant mixing emulsification is added in the virus solution after ultrafiltration concentration and formaldehyde solution inactivation so as to obtain a vaccine. The vaccine is utilized to inoculate a laying hen, eggs are obtained, yolks are separated, and the antibody is obtained after inactivation and extraction and rectification. The prepared antibody can prevent ducklings from the parvovirus infection because of muscovy duck parvoviruses, and the egg yolk antibody has the advantages of high efficiency, good safety, high protection rate and the like.

The invention discloses a soft-shelled turtle systemic septicemia spherical virus inactivated vaccine and its preparation method, and is characterized in that the vaccine contains the inactivated soft-shelled turtle systemic septicemia spherical virus with the collection number being CGMCCNo.5378. The preparation method comprises the following steps of: cutting internal organ of sick soft-shelledturtles into pieces, adding into a TNE buffer, centrifuging, taking a supernatant, centrifuging, taking a white precipitate, suspending in the TNE buffer, centrifuging the precipitate, taking the supernatant, and centrifuging to obtain a white precipitate, namely the purified virus; diluting the purified virus by the use of aseptic normal saline until the final concentration of viral protein reaches 0.5-1mg/ml, and adding 0.5% (final volume) of formalin for inactivation in a thermostat water bath cauldron; and completely emulsifying inactivated virus and a complete Freund's adjuvant accordingto the volume ratio of 1:1 to obtain the inactivated vaccine. The soft-shelled turtle systemic septicemia spherical virus inactivated vaccine proposed for the first time is safe and effective and hasstrong immunity, and the preparation method is simple and easy to operate.

The invention relates to the field of molecular biology and immunology and particularly discloses an Edwardsiella tarda recombinant subunit vaccine as well as a preparation method and application thereof. The vaccine has a base sequence in a sequence table SEQ ID No.1. The preparation method of the Edwardsiella tarda recombinant subunit vaccine comprises the following steps of constructing a vaccine antigen expression plasmid pETXV21, converting the vaccine antigen expression plasmid pETXV21 into escherichia coli BL21 (DE3), recovering a recombinant protein after induced expression, mixing the recombinant protein with a strain B187, and dissolving the mixture in PBS (Phosphate Buffer Saline) to obtain a vaccine mixed liquor which has the function of immune protection to Edwardsiella tarda. The vaccine mixed liquor is intraperitoneally injected to fishes to achieve the purpose of immune protection.

The invention provides a traditional Chinese medicine formula capable of improving animal immunity. The traditional Chinese medicine formula is prepared from, by weight, 25 parts of radix astragali, 25 parts of radix angelicae sinensis, 12.5 parts of herba epimedii, 25 parts of radix isatidis, and 12.5 parts of licorice. The invention also provides a preparation method of the traditional Chinese medicine formula. The preparation method comprises following steps: the ingredients are weighed at the above weight ratio, and are smashed using a pulverizer respectively; obtained powder products are sieved using a sieve of 150 meshes, and are mixed using a V-type mixer for 15min; and then an obtained product is subjected to subpackage, and is sealed. The traditional Chinese medicine formula is capable of repairing immune system and improving body immunity, releasing immunosuppression, improving immune effect, reducing medicine dosage, and extending drug application range, and is suitable to be applied to chicken, pigs, horses, cattle, sheep, ducks, geese, and rabbits.

A process for preparing the vaccine of chicken coccidiosis includes such steps as separating the oogonia from chicken coccidian, culturing in the solution of potassium bichromate until more than 90% of oogonia become spores, storing in refrigerator at 4 deg.C, adding distilled water, centrifugal removal of potassium bichromate solution, counting, ice bath, and ultrasonic weakening.

The invention relates to a traditional Chinese medicinal composition used as both food and medicine for treating porcine respiratory tract infection, and a preparation method thereof. The medicinal composition comprises, by weight, 100 to 120 parts of cordate houttuynia, 40 to 60 parts of pyrrosia leaf, 40 to 60 parts of perilla fruit, 120 to 140 parts of gypsum, 40 to 60 parts of white mustard seed, 15 to 35 parts of licorice, 40 to 60 parts of aster, 40 to 60 parts of radix stemonae, 40 to 60 parts of platycodon grandiflorum, 100 to 120 parts of semen raphani, and 15 to 35 parts of herba ephedrae. All the above raw materials are prepared according to the weight proportion, and ground into fine powder to be mixed in pig feed to feed pigs. The traditional Chinese medicinal composition provided by the invention mainly cures diseases with the main symptoms of cough and asthma caused by porcine respiratory tract infection, has the advantages of fast curative effect, short course of treatment, no recurrence, and no toxic side effect, and the total effective rate is more than 99%.

The invention discloses an intramolecular adjuvant-containing recombinant gene for preventing and treating helicobacter pylori, protein and a biological product. the multi-target recombinant gene comprises a multi-target fusion polypeptide having an amino acid sequence shown in a SEQ ID NO:2, a multi-target recombinant gene for coding the multi-target fusion polypeptide having the nucleotide sequence shown in a SEQ ID NO:2, and invention also has an application of a specific antibody of the above multi-target recombinant gene or the above multi-target fusion polypeptide or the multi-target fusion polypeptide as the biological product for preventing and treating helicobacter pylori infection. According to the invention, an intramolecular adjuvant CTB for removing signal peptide is added at N segment of a recombinant helicobacter pylori multi-target fusion polypeptide (rIB), so that prevention and treatment effect for the helicobacter pylori infection can be increased.

The invention discloses an immune-enhanced recombinant PRRSV (Porcine Reproductive and Respiratory Syndrome Virus)-like particle subunit vaccine. The immune-enhanced recombinant PRRSV virus-like particle subunit vaccine consists of PRRSV-like particles and a compound immunologic adjuvant. The immune-enhanced recombinant PRRSV-like particle subunit vaccine disclosed by the invention has the beneficial effects that by the means of gene engineering, PRRSV GP5-M gene is modified, and the PRRSV-like particles with high immunogenicity are prepared by constructing rhabdovirus expression vectors. In addition, by improvement on the immunologic adjuvant, the immune-enhanced recombinant PRRSV-like particle subunit vaccine is obtained and the subunit vaccine has a better immune effect.