Protective agent for vacuum freeze drying of lactobacillus johnsonii, and application thereof

A technology of vacuum freeze-drying and Lactobacillus johnsonii, applied in the biological field, can solve the problems of high cost, limited application of protective agents, and few studies, and achieves the effects of low cost, beneficial utilization efficiency and high protection rate

Active Publication Date: 2012-05-16
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on lyoprotectants is mainly concentrated in the field of medicine and medicine. There are not many studies on lyoprotectants used in the production of microbial feed additive live bacteria preparations, and the results vary greatly. There is a disadvantage of low viable count
There are sorbitol substances in some protective agents, and the protective effect has been improved to a certain extent, but the cost of sorbitol substances is relatively high, which limits its application in agricultural production and currently there is no protective agent for vacuum freeze-drying of Lactobacillus johnsonii

Method used

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  • Protective agent for vacuum freeze drying of lactobacillus johnsonii, and application thereof
  • Protective agent for vacuum freeze drying of lactobacillus johnsonii, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1- Embodiment 18

[0020] (1) Preparation of seed base

[0021] Pick a single colony of Lactobacillus johnsonii into 50 mL of MRS medium (10 grams of peptone, 10 grams of meat extract, 5 grams of yeast extract, 2 grams of dipotassium hydrogen phosphate, 2 grams of diammonium citrate, acetic acid 5 grams of sodium, 20 grams of glucose, 1 mL of soil temperature, 0.58 grams of magnesium sulfate heptahydrate, 0.25 grams of manganese sulfate tetrahydrate, 1 L of distilled water, pH6.2-6.4), 37 degrees constant temperature culture for 18 hours, and then 5% inoculum Transfer to 500mL MRS medium, continue to culture at 37 degrees for 18 hours, the value OD 600nm If it is 5.0, the seed base is obtained.

[0022] (2) High-density fermentation

[0023] 1% of the inoculum size was used to insert the seed base into a 5L fermenter. During the fermentation process, the tank pressure was maintained at 0.05 MPa with nitrogen, the rotation speed was 50 rpm, the temperature was 37 degrees, and ammonia water was ...

Embodiment 19

[0031] Example 19 Comparison of protective effects of different protective agents on Lactobacillus johnsonii

[0032] The present invention has studied under equal experimental conditions except that protecting agent, other experimental materials and consumption are all the same as embodiment 1, under the same situation of experimental method also with embodiment 1, the protecting agent in the prior art and the protecting agent of the present invention protective effect, etc. The results are shown in Table 2.

[0033] Table 2 Comparison of protective effects and costs of different protective agents for Lactobacillus johnsonii

[0034]

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PUM

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Abstract

The invention belongs to the technical field of biology, and in particular relates to a protective agent for vacuum freeze drying of lactobacillus johnsonii, and application thereof. The protective agent provided by the invention contains no sorbitol substances, has lower cost, can provide effective protection for lactobacillus johnsonii in a vacuum freeze drying process, and is favorable to improvement of the utilization efficiency of a lactobacillus microbial feed additive, the protective rate of the protective agent is high, and the yield of produced live bacteria is above 109.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a protective agent for vacuum freeze-drying of Lactobacillus johnsonii and its application. Background technique [0002] Lactobacillus johnsonii (Lactobacillus johnsonii) is an important lactobacillus, which belongs to probiotics. Its beneficial function to animals is mainly reflected in the following three aspects: 1. Regulate the natural defense system and immune function of animals; 2. Prevent animal-specific Dermatitis effect; 3. Exclusive competitive effect. However, Lactobacillus johnsonii has strict requirements on growth conditions and is easily inactivated during production, preparation and storage. [0003] Vacuum freeze-drying technology is one of the most effective ways to preserve fermented probiotics. However, during the vacuum freeze-drying process, because there is still a lot of water in the cells, when the cells are frozen at low temperature, the water...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/225
Inventor 张军汪攀孙晓雯闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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