Egg yolk antibody for preventing and curing muscovy duck parvovirus diseases

A technology of Muscovy duck parvovirus and egg yolk antibody, which can be used in antiviral agents, antiviral immunoglobulins, and egg-derived immunoglobulins, etc., and can solve the problems of virus spread and harm.

Active Publication Date: 2014-06-04
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the dead muscovy ducks are not handled properly, the virus will often spread and cause greater harm. At present, there is a live vaccine to prevent the disease on the market, but there is no fast and effective drug or method to prevent and treat the muscovy duck parvovirus disease

Method used

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  • Egg yolk antibody for preventing and curing muscovy duck parvovirus diseases
  • Egg yolk antibody for preventing and curing muscovy duck parvovirus diseases
  • Egg yolk antibody for preventing and curing muscovy duck parvovirus diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Preparation of virus liquid for seedling production Inoculate 12-14-day-old susceptible muscovy duck embryos into the allantoic cavity of the virus species YBMDV strain for production, 0.2ml per embryo, incubate at 36-37°C, and inspect the embryos twice a day . Choose goose embryos that died within 48 to 120 hours after inoculation, and the embryo body had extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and choriourinium with mild edema, and the allantoic fluid and goose embryo body were put into the tissue and mashed Pulverize in the machine, take the pulverized tissue and mix it with saline at a ratio of 1:2; place it at 2-8°C for 4-12 hours, collect allantoic fluid, amniotic fluid and embryo body (remove the head and limbs). Homogenize with embryo fluid, freeze-thaw 3 times, centrifuge at 4000r / min for 30min, take supernatant and mix it in a sterile container, store at 2-8°C. (See Table 1).

[0018] Table 1: Preparation of virus ...

Embodiment 2

[0045] Example 2: Preparation of Muscovy Duck Parvovirus Egg Yolk Antibody

[0046] 1. Preparation of Vaccine Immune Eggs

[0047]Immune laying hens with the inactivated Muscovy duck parvovirus vaccine prepared above, and inject 1 part of Muscovy duck parvovirus disease live vaccine subcutaneously into the neck of each chicken for the first immunization, and perform the second immunization after 14 days, and inject subcutaneously into the neck of each chicken 2.0ml inactivated vaccine, 14 days after the second immunization for the third immunization, 2.0ml inactivated vaccine subcutaneously injected into the neck of each chicken, and the third immunization 14 days after the third immunization, 2.0ml subcutaneously injected into the neck of each chicken For the inactivated vaccine, 14 days after the fourth immunization, the egg yolk was collected to determine the titer of Muscovy duck parvovirus AGP antibody at 1:64.

[0048] 2. Yolk antibody production (see Table 4)

[0049]...

Embodiment 3

[0062] Embodiment 3: egg yolk antibody finished product inspection

[0063] (1) Properties This product is light yellow transparent liquid. The pH value is 7.1.

[0064] (2) The filling volume inspection is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", which is in compliance with the regulations.

[0065] (3) The sterility test is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and the growth is sterile.

[0066] (4) For safety inspection, 10 susceptible young muscovy ducks aged 1 to 3 days were used, and 2.0ml of this product was injected into each muscle point. On the 14th day of observation, all susceptible ducklings were healthy and alive.

[0067] (5) Effectiveness test (see Table 5)

[0068] 1) Serological methods The titers of yolk antibody against parvovirus in Muscovy ducks were all 1:64.

[0069] 2) Immune challenge method: 30 5-day-old susceptible muscovy ducks were randomly div...

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Abstract

The invention aims to provide an egg yolk antibody for preventing and curing muscovy duck parvovirus diseases, and the egg yolk antibody is prepared by using muscovy duck parvoviruses the preserving number of which is CGMCC (China General Microbiological Culture Collection Center) No.8504 as an antigen. The egg yolk antibody is characterized in that a muscovy duck parvoviruse YBMDP plant is inoculated with a muscovy duck embryo, a die embryo body and embryo liquid are respectively obtained and are grinded and are subjected to freeze thawing so as to obtain a virus solution, and the oil adjuvant mixing emulsification is added in the virus solution after ultrafiltration concentration and formaldehyde solution inactivation so as to obtain a vaccine. The vaccine is utilized to inoculate a laying hen, eggs are obtained, yolks are separated, and the antibody is obtained after inactivation and extraction and rectification. The prepared antibody can prevent ducklings from the parvovirus infection because of muscovy duck parvoviruses, and the egg yolk antibody has the advantages of high efficiency, good safety, high protection rate and the like.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, in particular to an egg yolk antibody used for the prevention and treatment of parvovirus disease in muscovy ducks. Background technique [0002] Muscovy duck parvovirus disease is an acute or subacute infectious disease that affects young muscovy ducks. It is highly contagious, with high morbidity and mortality, and often causes serious economic losses. It mainly occurs at the age of 1 to 3 weeks. The young muscovy ducks, especially the young muscovy ducks about 10 days old, basically do not get sick when they are more than 3 weeks old. An infectious disease characterized by panting, diarrhea, pancreas necrosis and hemorrhage in young muscovy ducks. The disease first appeared in Fujian, China, and the Brittany region of western France in the mid-to-late 1980s. It was not recognized until the early 1990s that it was an independent disease different from gosling plague, with a mortal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/02A61P31/20
Inventor 宫晓徐保娟宋新宇李陆梅胡潇张维王龙
Owner YEBIO BIOENG OF QINGDAO
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