81 results about "Conceptus" patented technology

The present invention provides methods of inducing an immune response against Clostridium species in birds, for protecting birds from Clostridium infection, and/or for protecting birds from related disorders such as necrotic enteritis. The methods can be practiced in ovo and/or post-hatch. The invention further provides compositions and methods for delivery of a composition of this invention in ovo directly to the embryo body.

he present invention is of a method of dynamically generating human embryoid bodies which can be used for generating lineage specific cells and cell lines. Specifically, the present invention can be used to generate ESC-differentiated cells for cell-replacement therapy.

The present invention provides a method that embryo of human embryonic stem cells (HES) is directionally induced and divided into liver cells. First, the biological property of high degree of self-regeneration multiplication of HES cell is made use of, the collagenase IV digestion is adopted for transfer of culture and augmentation, thereby obtaining a plurality of stem cells; second, the HES cell is inoculated into a cell utensil with lower adsorbability to form a mature imitated embryonic plant (EB); third, the mature cystic EB is digested with 0.25 tryptic enzyme to 0.02 percent EDTA to a single cell, and then is inoculated to a tissue culture dish which is packed with I-shaped collagen in advance, solution culture is induced under the condition of dexamethasone and human trypsin, and the differentiation towards the liver cell direction is observed and judged. The prevent invention provides the method that the HES is efficiently divided into liver cells, at the same time, the method makes the follow-up judging work more simply and more efficient, and lay a foundation for the stem cellular transplantation or biologic artificial liver curing the disease such as serious hepatitis, hepatic failure and so on.

A preparation method of a purified vitelline freezing antibody for chickens infectious bursal disease is characterized in that: high purity antigens are adopted to vaccinate IBDV B87 to SPF chickens, then velvet urine, idiosome, velvet urine membrence are acquired, grinded, filtered and inactivated to antigens, and the steps are: producing immune eggs, collecting high immune egg, separating vitelline, diluting with distilled water, inactivating, extraction, decentering, inactivating, freezing and checking; the eggs is radiated by Co60 to kill viruses and bacterium, and finally froze by novel immuno-enhancer - Astragali Polysaccharoses and preserved under a temperature of 2-8 DEG C. Composite drugs for prevention and therapy are prepared by adopting the vitelline antibody for chickens infectious bursal disease as major components and cooperating with a novel immuno-enhancer, to decrease the jeopardy of the chicken breeding industry, and improve the living level of people and benefit mankind.

The invention provides an inducing medium for inducing multipotential stem cells to form mesoblastic cells. The inducing medium is prepared from the following components with the corresponding concentrations: 5-15 mM of LiCl, 0.5-2 % of glutamine, 0.5-2 % of non-essential amino acid, 5-15 [mu] M of CHIR99021, 8-15 ng/ml of bFGF, 0.05-0.2 mmol/l of beta mercaptoethanol, 0.2-0.6 [mu] g/ml of hydrocortisone, 4-10 [mu] g/ml of insulin, 80-120 [mu] g/ml of transferrin, 8-12 ng/ml of sodium selenite, 0.05-0.2 mg/ml of penicillin, and 0.05-0.2 mg/ml of streptomycin. For the inducing medium, on the basis of the DMEM/F12 culture medium, some specific cytokines and supplements are added, and thus the probability of inducing the multipotential stem cells to differentiate towards the mesoblastic cells is improved. In addition, the invention provides an inducing method for inducing the multipotential stem cells to form the mesoblastic cells, according to the method, the simple embryoid body for inducing the multipotential stem cells is inoculated to the inducing medium for induced culture, and thus the multipotential stem cells efficiently differentiate to form the mesoblastic cells.

The invention aims to provide an egg yolk antibody for preventing and curing muscovy duck parvovirus diseases, and the egg yolk antibody is prepared by using muscovy duck parvoviruses the preserving number of which is CGMCC (China General Microbiological Culture Collection Center) No.8504 as an antigen. The egg yolk antibody is characterized in that a muscovy duck parvoviruse YBMDP plant is inoculated with a muscovy duck embryo, a die embryo body and embryo liquid are respectively obtained and are grinded and are subjected to freeze thawing so as to obtain a virus solution, and the oil adjuvant mixing emulsification is added in the virus solution after ultrafiltration concentration and formaldehyde solution inactivation so as to obtain a vaccine. The vaccine is utilized to inoculate a laying hen, eggs are obtained, yolks are separated, and the antibody is obtained after inactivation and extraction and rectification. The prepared antibody can prevent ducklings from the parvovirus infection because of muscovy duck parvoviruses, and the egg yolk antibody has the advantages of high efficiency, good safety, high protection rate and the like.

The invention discloses a pluripotent stem cells directional differentiation method. The method is characterized in that pluripotent stem cells are cultured under cell culture environment of non exogenous hematopoietic cytokines, non serum, non matrix cells and clear component, after embryoid is formed, a vascular endothelial growth factor and bone morphogenetic protein 4 are added, the embryoid is attached on the surface spread with collagen IV, mesoderm differentiation is induced, and hemopoietic progenitor cells and blood corpuscles are generated. The cell culture system with determined component enables directional differentiation on the pluripotent stem cells, has the advantages of low cost, simple operation, high repeatability and ordered differentiation process, can massively generate hemopoietic progenitor cells and blood corpuscles, and has important meaning for researching a blood growth process mechanism.

The invention relates to the field of stem cell biology, in particular to midbrain dopaminergic neural precursor cells and a preparation method and application thereof. The midbrain dopaminergic neural precursor cell expresses Lmx1a+, Foxa2+, En1+ and Otx2+, wherein the midbrain dopaminergic neural precursor cell does not express one or more of Nkx2.1, DBX1 and GBX2. The method for preparing the mesencephalic dopaminergic neural precursor cell comprises the following steps: embryoid bodies are formed, midbrain floor cells are obtained, and midbrain dopaminergic neural precursor cells are obtained. By adopting the preparation method, the mesencephalic dopaminergic nerve cells can be rapidly and efficiently differentiated from the human pluripotent stem cells, and the problems are solved that an existing differentiation method is long in time, unstable in effect and low in efficiency, the culture system containing serum or the trophoblast cells are not conducive to the production of subsequent clinical-grade cell preparations and the like.

The invention discloses an optimized mice somatic cell nuclear transplantation method. According to the mice somatic cell nuclear transplantation method, three kinds of cells, including cumulus cell, fetal fibroblasts, and embryonic stem cell, are adopted to construct reconstructed embryos; the activation efficiencies of three reconstructed embryos in different activation mediums are compared, development efficiencies in different solutions are compared, and pregnancy efficiencies of different embryo transplantation methods are compared; it is concluded that calcium-free KSOM activation medium is a universal high efficiency activation medium, and the activation efficiency is about 93.5%; KSOM-AA culture medium is suitable for in vitro culture of the reconstructed embryos of cumulus cells and embryonic stem cells, and the best culture medium for fetal fibroblasts is alpha MEM culture medium; when the reconstructed embryos are at 2-cell period, nuclear transplanted animals can be obtained via transplantation of embryos through bilateral fallopian tubes, wherein for each fallopian tube, transplantation of 15 embryos is carried out. The optimized mice somatic cell nuclear transplantation method is capable of increasing reconstructed embryo cleavage rate, blastula development rate, cloning animal birth rate, and survival rate, so that the success rate of obtaining embryonic stem cells from the reconstructed embryos is increased.

The invention discloses a neural crest spectrum mesenchymal stem cell derived from pluripotent stem cells and an induced differentiation method thereof. The induced differentiation method of the neural crest spectrum mesenchymal stem cell comprises the following steps: dissociating the pluripotent stem cells to obtain cell aggregates, carrying out suspension culture to form embryoid bodies, replacing a culture solution with a neural crest cell culture solution, carrying out suspension culture of the embryoid bodies, collecting the embryoid bodies and replacing the culture solution every day; enabling the embryoid bodies to grow for a certain time by adhering to the wall after culturing for a certain time; digesting and collecting cells; culturing the collected cells by enabling the cells to adhere to the wall, replacing the culture solution with a mesenchymal stem cell culture solution, carrying out passage culture, then detecting the mesenchymal cell phenotype to obtain the neural crest spectrum mesenchymal cell. Through the method, the defects of heterogeneity and hybridity between adult-derived mesenchymal stem cells and other mesenchymal stem cells derived from the pluripotentstem cells of non-finite induction pathways can be solved; the prepared neural crest spectrum mesenchymal stem cell has higher immunoregulation and osteogenic differentiation capabilities; the standardized induced differentiation process is capable of ensuring that cell populations prepared between different batches have good consistency.

The invention relates to a chick embryo allantoic cavity inoculation method for improving separation rate of bird flu viruses. The invention is characterized in that: a pinhole is arranged on the side of an air chamber of a chick embryo and taken as a pressure-relief vent; a pinhole is arranged on an embryo body of the chick embryo close to an allantoic cavity and taken as a needle insertion hole; a needle is directly inserted into the allantoic cavity for inoculation of sample by 0.5 to 1.0 milliliter per embryo. The invention reaches the goal of increasing viral load by increasing inoculum concentration, thereby the separation rate of viruses is improved.

The invention relates to a preparation method of divalent inactivated vaccines for duck virus hepatitis. According to the invention, the preparation method comprises the following steps of: screening duck virus hepatitis type I YBH1 strains and novel YBHX strain vaccines with good immunogenicity from preponderant popular strains so as to prepare strains, respectively inoculating the vaccines on a chicken embryo and a duck embryo, respectively harvesting embryoid bodies and embryoid fluid of dead embryos, collecting a viral solution after the embryoid bodies and the embryoid fluid are ground and frozen-thawed, and adding an oil adjuvant for mixing and emulsifying to obtain the vaccines after the viral solution is carried out ultrafiltration and concentration and is inactivated through a formaldehyde solution. The vaccines can carry out immunization for animals and can simultaneously prevent the duck virus hepatitis caused by type I duck virus hepatitis and novel duck virus hepatitis, and the divalent inactivated vaccines for the duck virus hepatitis have the advantages of high efficiency, good safety and high protective ratio and the like.

The invention provides a culture solution for improving the development quality of cell cloning embryos and in-vitro fertilized embryos of a cow body and a culture method. The culture solution comprises the following components: BME (Basal Eagle Medium) with the volume fraction being 2%, MEM (Minimum Essential Medium) with the volume fraction being 1%, 1mmol/L of glutamine, 8mg/mL of BSA (Bovine Serum Albumin), 50mu g/mL of gentamicin and 50mu g/mL of cephalosporin. After the cow embryo is cultured to the 96th hour, KSR (Knockout Serum Replacement) with the volume fraction being 5% is added. The culture solution and the culture method provided by the invention have the beneficial effects that the culture solution is utilized for in-vitro culture of the cell cloning embryos and the in-vitrofertilized embryos of the cow body, a result shows that the development rate and the quality of the blastosphere are respectively higher than those of a control group; by application of the culture solution and the culture method, the cell cloning embryos or the in-vitro fertilized embryos of the cow body can be obtained with high efficiency.