Culture solution for improving development quality of cell cloning embryos and in-vitro fertilized embryos of cow body and culture method

Abstract

The invention provides a culture solution for improving the development quality of cell cloning embryos and in-vitro fertilized embryos of a cow body and a culture method. The culture solution comprises the following components: BME (Basal Eagle Medium) with the volume fraction being 2%, MEM (Minimum Essential Medium) with the volume fraction being 1%, 1mmol/L of glutamine, 8mg/mL of BSA (Bovine Serum Albumin), 50mu g/mL of gentamicin and 50mu g/mL of cephalosporin. After the cow embryo is cultured to the 96th hour, KSR (Knockout Serum Replacement) with the volume fraction being 5% is added. The culture solution and the culture method provided by the invention have the beneficial effects that the culture solution is utilized for in-vitro culture of the cell cloning embryos and the in-vitrofertilized embryos of the cow body, a result shows that the development rate and the quality of the blastosphere are respectively higher than those of a control group; by application of the culture solution and the culture method, the cell cloning embryos or the in-vitro fertilized embryos of the cow body can be obtained with high efficiency.

Description

technical field [0001] The invention belongs to the field of livestock embryo engineering, in particular to a culture solution and a culture method for bovine somatic cell cloned embryos and in vitro fertilized embryos. Background technique [0002] The in vitro culture of livestock embryos is a technology with great research and commercial value, which can be applied to embryo in vitro transplantation, animal gene editing and breeding, and the expansion of excellent livestock breeds. However, the development quality of bovine in vitro embryos is still not high compared with bovine in vivo embryos, mainly reflected in the few blastocysts obtained, poor shape, small number of blastocyst cells, high number of apoptotic cells, low pregnancy rate, and prolonged pregnancy time. , giant fetuses, and unbalanced sex ratio at birth, etc., therefore, significantly restrict the wide application of this technology. [0003] Knockout serum replacement (KSR) is a serum-free nutrient with...

AI Technical Summary

Problems solved by technology

However, the development quality of bovine in vitro embryos is still not high compared with bovine in vivo embryos, mainly reflected in the few blastocysts obtained, poor shape, small number of blastocyst cells, high number of apoptotic cells, low pregnancy rate, and prolonged pregnancy time. , giant fetuses, and unbalanced sex ratio at birth, etc., therefore, significantly restrict the wide application of this technology

Images