A kind of goat cell clone embryo culture medium and culture method
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A technology for cloning embryos and culturing methods, applied in the field of bioengineering, can solve the problems of low development rate of cloned embryos
Active Publication Date: 2021-05-11
FUYANG NORMAL UNIVERSITY
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[0005] The invention provides a goat cell cloning embryo culture solution, which is highly targeted and solves the problem that the development rate of cloned embryos generally required by M16 medium is relatively low
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Embodiment 1
[0021] A kind of goat cell clone embryo culture liquid, comprises A liquid and B liquid, contains the ferrous nitrate of 0.2mg, the potassium chloride of 350mg, the citric acid of 100mg, the sodium dihydrogen phosphate of 100mg, the remaining The amount is DMEM high-sugar liquid medium;
[0022] Each liter of the liquid B contains 15 mg of fetal bovine serum, 150 mg of malic acid, 0.2 g of sodium chloride, and the balance is M16 liquid medium.
[0023] The malic acid is L-malic acid.
Embodiment 2
[0025] A kind of goat cell clone embryo culture liquid, comprises A liquid and B liquid, contains the ferrous nitrate of 0.2mg, the potassium chloride of 350mg, the citric acid of 120mg, the sodium dihydrogen phosphate of 100mg, the remaining The amount is DMEM high-sugar liquid medium;
[0026] Each liter of the liquid B contains 17 mg of fetal bovine serum, 120 mg of malic acid, 0.2 g of sodium chloride, and the balance is M16 liquid medium.
[0027] The malic acid is D-malic acid.
Embodiment 3
[0029] A kind of goat cell clone embryo culture liquid, comprises A liquid and B liquid, contains the ferrous nitrate of 0.2mg, the potassium chloride of 350mg, the citric acid of 100mg, the sodium dihydrogen phosphate of 110mg, the remaining The amount is DMEM high-sugar liquid medium;
[0030] Each liter of the liquid B contains 10 mg of fetal bovine serum, 130 mg of malic acid, 0.2 g of sodium chloride, and the balance is M16 liquid medium.
[0031] The malic acid is the mixture DL-malic acid.
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Abstract
The invention belongs to the technical field of bioengineering, and discloses a goat cell clone embryo culture solution and a culture method thereof. The goat cell clone embryo culture solution includes A liquid and B liquid, and each liter of the A liquid contains 0.2 mg of nitric acid Ferrous iron, 350 mg of potassium chloride, 100-120 mg of citric acid, 100 mg of sodium dihydrogen phosphate, and the balance is DMEM high-sugar liquid medium. The culture medium improves the DMEM medium and M16 medium of the prior art, prepares the A liquid and B liquid of the culture medium, and then adopts the staged culture cell, the number of two-cell embryos, the number of four-cell embryos, the number of morula embryos, The number of blastocysts and the number of blastocysts survived after implantation were higher. The in vitro development rate of goat fertilized eggs was generally higher than that in DMEM medium and M16 medium.
Description
technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a goat cell cloned embryo culture solution and a culture method. Background technique [0002] Goats are an important economic animal that produces meat, velvet, milk and other economic products, especially goat milk, which has huge market potential. If high-quality genetic resources of goat breeds are obtained and maintained, it is necessary to improve the existing population and become a goat The urgent problem of large-scale farming. [0003] Embryo cloning is a method of cloning, the most commonly used is somatic cell cloning technology, which refers to the method of nuclear transfer, cell disassembly or cell recombination technology, the enucleation of oocytes as nuclear receptors, somatic cells or The nuclear plastid containing a small amount of cytoplasm is used as the nuclear donor, and the nuclear donor is transferred into the nuclear recipient to con...
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