243 results about "Reticulum cell" patented technology

The invention relates to the technical field of biological medicine, in particular to a ferroptosis inhibition type phospholipid-like material and application thereof. The ferroptosis inhibition type phospholipid-like material is one of the following structural general formulas (1)-(5). The biomimetic phospholipid-like ferroptosis inhibitor has a long retention characteristic in main positions (cell membranes, endoplasmic reticulum and other organelle membranes) of cell ferroptosis, so that the ferroptosis inhibition efficiency is remarkably improved. The novel phospholipid-like material not only can be used as an active drug molecule, but also can be used as a pharmaceutic adjuvant for constructing drug delivery carriers such as lipidosome and micelle and implant coatings, and is suitable for various administration routes such as oral administration, injection and local administration. The novel biomimetic ferroptosis inhibitor can efficiently relieve cell ferroptosis and has a wide application prospect in the field of treating or retarding ferroptosis-related diseases.

The invention relates to the technical field of antitumor drugs, in particular to a Ru (II) complex and a preparation method and application thereof, and the Ru (II) complex has a structure as shown in a formula I. The Ru (II) complex synthesized by the method disclosed by the invention not only has relatively high cytotoxicity, but also has good photodynamic antitumor activity. After the complex is illuminated, the efficiency of the complex entering cells in an active transportation mode can be accelerated, mitochondria and endoplasmic reticulum are targeted at the same time, mitochondrial membrane potential decline and endoplasmic reticulum stress are caused to form a dual organelle damage effect, immunogenic cell death is caused, the tumor microenvironment is adjusted, and the tumor cell immunogenicity is improved. The enrichment of dendritic cells (DCs) in tumor cells is realized, the chemotactic activity of effector T cells is improved, the proportion of CD4 < + > and Foxp3 < + > cell populations is reduced, and finally the anti-tumor immune response is activated. Meanwhile, the complex provided by the invention can also induce the cell to generate pan apoptosis, retard the cell cycle in the G2 / M period and enhance the effect of the complex in inhibiting tumor proliferation.

The invention relates to the technical field of biological medicines, in particular to application of an EIF3A inhibitor in preparation of a medicine for treating hepatic fibrosis. The EIF3A inhibitor relieves the hepatic fibrosis process by inhibiting EIF3A expression of hepatic tissue, relieving endoplasmic reticulum stress, inhibiting an NF-kappa B inflammation pathway and reducing pathological damage of hepatic cells, and the EIF3A inhibitor can be selected from a medicine for knocking down or knocking out EIF3A, a medicine for inhibiting EIF3A protein synthesis or a medicine for promoting EIF3A protein degradation. New mechanism research is provided for occurrence and development of hepatic fibrosis, hepatic cell damage and inflammation can be relieved through targeting EIF3A, hepatic fibrosis related pathological phenotypes are relieved, the protection and treatment effects are achieved, safety is higher, and therefore the application has good practical application value.

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of a scrophularia ningpoensis extract for relieving chemotherapy-induced acute kidney injury and application of the scrophularia ningpoensis extract in preparation of a product for relieving chemotherapy-induced acute kidney injury. The preparation method comprises the following steps: putting fresh ginseng into a high-temperature and high-pressure sterilization pot, steaming at 110-120 DEG C for 1-3 hours, taking out, cooling to room temperature, and repeating the steaming cooling process for 2-5 times; and crushing the treated ginseng materials, adding a mixed strain of saccharomyces cerevisiae and lactobacillus plantarum in a mass ratio of 1: 1, fermenting for 24-72 hours, filtering fermentation liquor, and drying filter residues to obtain the black ginseng extract. The cisplatin-induced acute kidney injury is prevented and / or treated by improving oxidative stress and mitochondrial functional activity, reducing the content of lipid peroxide, inhibiting expression of inflammatory factors TNF alpha, IL1beta and COX2 and remarkably reducing the cell apoptosis rate and the level of inflammatory factors related to endoplasmic reticulum stress.

A model that closely resembles human excessive myopia can be prepared by mounting a minus lens (2) and a protector (4) to a juvenile mouse, the minus lens having an angle and a width adjustable in response to growth of the mouse. Further, this model analysis shows that myopia induction causes endoplasmic reticulum stress in a sclera and the endoplasmic reticulum stress induces myopia. Furthermore, it is revealed that an endoplasmic reticulum stress suppressant, particularly, phenylbutyrate and tauroursodeoxycholic acid act as a myopia prevention / suppression agent.

The invention relates to the technical field of medicines, in particular to a composite nano vaccine and application thereof. The composite nano vaccine is prepared by coupling a natural compound with endoplasmic reticulum stress induction capability on the surface of Ga-MOF nano particles. The composite nano vaccine can significantly enhance the anti-tumor effect through a ferroptosis mechanism, an endoplasmic reticulum stress and immunogenic cell death mechanism and a cryoablation technology synergistic effect mechanism, reverses an immunosuppressed tumor microenvironment, inhibits the growth of primary tumors and distant metastatic tumors, and has a good anti-tumor effect. A brand new strategy is provided for comprehensive treatment of tumors, and the application prospect is wide.

The invention discloses a method for efficiently expressing laccase by regulating endoplasmic reticulum stress reaction of coprinus cinereus, and belongs to the field of gene engineering and fermentation engineering. According to the invention, by regulating endoplasmic reticulum stress reaction action genes der1, Anhac1 and Cchac1 of filamentous fungi coprinus cinereus, recombinant extracellular expression of laccase Lcc5 is finally improved and regulated. Wherein the extracellular laccase activities of the recombinant bacteria can be respectively improved by 18%, 70% and 26% through interference of der1, overexpression of Anhac1 and overexpression of Cchac1, and the obtained extracellular laccase activities are respectively 31.4 U / mL, 45.1 U / mL and 33.6 U / mL, which is the highest level of coprinus cinereus homologous recombination expression laccase Lcc5 reported at present. The invention firstly indicates that the regulation and control of the coprinus cinereus endoplasmic reticulum stress reaction can promote the efficient expression of laccase in coprinus cinereus, and the invention further promotes the development of a coprinus cinereus expression system.

The invention discloses application of an Hspa5 inhibitor in preparation of a medicine for preventing or treating anxiety disorder. An anxiety mouse model is constructed through chronic constraint stress (CRS), and in combination with medial amygdala kernel (MeA) transcriptome sequencing and qPCR verification, it is found that the endoplasmic reticulum molecular chaperone Hspa5 is remarkably up-regulated in the anxiety state. Furthermore, an Hspa5 specific inhibitor HA15 is locally injected into a MeA brain region, so that the anxiety-like behavior induced by the CRS is remarkably improved, and the exploration time of an open field experiment central region, the exploration time of an open arm of an elevated cross labyrinth and the exploration time of a bright box of a bright-dark box experiment are prolonged. The invention discloses the function of Hspa5 as a novel anti-anxiety target for the first time, and provides a direct experimental basis and a transformation direction for developing a novel anti-anxiety drug which is non-monoamine and targets an endoplasmic reticulum homeostasis.

The present invention relates to a novel purine derivative compound and a composition for enhancing an immune response comprising same as an active ingredient. The compound of the present invention not only has a nanomolar EC50 value for TLR7, which is an intracellular membrane receptor of immune cells, but also has high selectivity for TLR7 compared to TLR8, which has a similar structure and is mainly distributed in the endoplasmic reticulum (ER), thereby being able to induce sustained immune activation. Therefore, the compound of the present invention can be effectively used as an efficient vaccine adjuvant composition against various RNA viruses including influenza virus, SARS-CoV-2, and hepatitis C virus.

To provide an endoplasmic reticulum stress suppressing composition and a liver function improving composition.SOLUTION: A composition for suppressing endoplasmic reticulum stress and / or a composition for improving hepatic functions, comprising one or more plants selected from the group consisting of Plantago asiatica, Hamilton's spindle tree, panicled hydrangea, Japanese plum yew, Japanese mallotus, and Papaver rhoeas, and / or an extract thereof.SELECTED DRAWING: None

An endoplasmic reticulum-targeted long-wavelength fluorescent probe for detecting hydroxyl free radicals, and its preparation method and application. The structure of the fluorescent probe is shown in Formula I. The present invention also provides a method for preparing the fluorescent probe and its application in hydroxyl free radical detection. The fluorescent probe provided by the present invention can sensitively and specifically detect hydroxyl free radicals in the long analytical wavelength region of 640 nm. It also has the advantages of selectively targeting the endoplasmic reticulum, low biological background fluorescence signal, minimal photodamage to biological samples, good biocompatibility, and easy preparation and use. It has great application prospects in the fields of chemistry, biology, medicine, etc.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of an endoplasmic reticulum protein antioxidant (reducing agent) in prevention and treatment of bombyx mori nuclear polyhedrosis virus. A screening test finds that the related endoplasmic reticulum protein antioxidants beta-mercaptoethanol (BME), dithiothreitol (DTT) and / or tris (2-carboxyethyl) phosphine (TCEP) have the effect of inhibiting the bombyx mori nuclear polyhedrosis virus (BmNPV), and the antioxidants interfere the replication and proliferation of the virus by destroying disulfide bonds in key proteins of the BmNPV, so that the bombyx mori nuclear polyhedrosis virus can be inhibited. The stability and activity of virions are directly influenced, so that the infection of the viruses on the silkworms is inhibited, a brand new thought is provided for the development of antiviral drugs of the silkworms, and the application value is good.

The invention discloses an XXM-ER mutant for regulating and controlling plant endoplasmic reticulum calcium ion homeostasis, nucleic acid and application, and belongs to the technical field of biological engineering. The XXM-ER mutant has two amino acid mutations of H134Q and D156H on a light-operated cation channel protein ChR2, and the amino acid sequence of the mutant is as shown in SEQ ID NO. 1. The XXM-ER mutant disclosed by the invention has better endoplasmic reticulum localization, can realize precise regulation and control of plant calcium ion flow through illumination regulation and control, and has great application value in the aspects of plant cultivation, plant calcium signal research and the like.

The invention provides an AIE photosensitizer with an endoplasmic reticulum targeting function as well as a preparation method and application of the AIE photosensitizer. The AIE photosensitizer has the following structure shown in the specification. Under the action of illumination, TTP-ER can induce generation of a large amount of ROS in the toxoplasma gondii bodies, typical ultrastructure damage such as cell membrane rupture and collapse is caused, and rapid inactivation of the free toxoplasma gondii is achieved. Meanwhile, the photosensitizer treatment can significantly inhibit the formation of plaques of polypide bodies in host cells, reduce the invasion, replication and multiplication capacities, and show clear in-vitro toxoplasma gondii resistance activity.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PoPILS1a gene in regulation and control of plant seed development. The coding sequence of the PoPILS1a gene is as shown in SEQ ID NO: 1. According to the invention, the specific expression of the peony PoPILS1a in seed tissues is found for the first time, the expression levels are greatly different in different development stages, and the PoPILS1a protein is positioned on a cell endoplasmic reticulum. In an arabidopsis thaliana positive strain with over-expression of PILS1a, the epiphytic point between pods is obviously shortened, the number of pods with limited inflorescence length distribution is larger, meanwhile, the lengths and the weights of the pods at the corresponding positions are obviously different compared with those of a contrast, and it is clear that PILS1a can promote development of fruits and has good practical application value.

The invention discloses a nano-particle for inducing death of tumor immunogen cells, a preparation method and application. The preparation method of the nano-particles comprises the following steps: taking a BiOCl nanosheet as a carrier, enabling CeO2 to generate and crystallize in situ on the surface of the BiOCl nanosheet, and forming a BiOC / CeO2 heterojunction; the method comprises the following steps: carrying out a dehydration reaction on NH2-PEG2000-NH2 and DL lipoic acid to synthesize NH2-PEG2000-TA; the preparation method comprises the following steps: reacting NH2-PEG (Polyethylene Glycol) 2000-TA with a Pardaxin peptide of which the carboxyl is activated, so as to generate Pardaxin-PEG 2000-TA; the preparation method comprises the following steps of: coupling Pardaxin-PEG2000-TA (Polyethylene Glycol 2000-TA) and a BiOC / CeO2 heterojunction; the PBCP nanoparticles obtained by the preparation method promote the yield of active oxygen in the endoplasmic reticulum of tumor cells to be increased sharply, so that the endoplasmic reticulum stress is effectively excited, tumor immunogenic cells are induced to die, and the anti-tumor effect is achieved. The nanoparticle can be combined with the existing PD-1 / PD-L1 inhibitor for use, and shows a better anti-tumor effect than the single use of the PD-1 / PD-L1 inhibitor.

The invention belongs to the technical field of biological medicine and molecular biology, and particularly relates to application of MIGA2 serving as a target spot in screening or preparing a medicine for treating colorectal cancer. According to the invention, the correlation between MIGA2 and colorectal cancer progress is analyzed through large-queue bioinformatics; the influence of MIGA2 on the proliferation, migration and invasion ability of colorectal cancer cells is analyzed from an in-vitro cell model; analyzing a key molecular mechanism of MIGA2 playing a cancer inhibition function; the influence of MIGA2 on colorectal cancer tumor growth is analyzed in an in-vivo mouse model, the influence of MIGA2 mediated mitochondrial-endoplasmic reticulum contact on colorectal cancer tumor growth is analyzed, and it is verified in multiple dimensions that MIGA2 can become an effective treatment target of colorectal cancer.

This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.

The present invention provides a multiomics approach, which integrate single-cell RNA-sequencing (scRNA-seq) and spatiotemporal transcriptomics (ST) offering potential for dissecting transcriptional networks and revealing cell-cell interactions involved in biomolecular pathomechanisms. The present invention also provides a multimodal approach combining high-throughput scRNA-seq and ST to elucidate XLRS-specific transcriptomic signatures in two XLRS-like models with retinal splitting phenotypes, including genetically engineered (Rs1emR209C) mice and patient-derived retinal organoids harboring the same patient-specific p.R209C mutation. Through multiomics transcriptomic analysis, the endoplasmic reticulum (ER) stress / eIF2 signaling, mTOR pathway, and the regulation of eIF4 and p70S6K pathways as chronically enriched and highly conserved disease pathways between two XLRS-like models are identified. Western blots and proteomics analysis validated the occurrence of unfolded protein responses, chronic eIF2α signaling activation, and chronic ER stress-induced apoptosis. Furthermore, therapeutic targeting of the chronic ER stress / eIF2α pathway activation synergistically enhanced the efficacy of AAV mediated RS1 gene delivery, ultimately improving bipolar cell integrity, postsynaptic transmission, disorganized retinal architecture and electrophysiological responses. Collectively, the complex transcriptomic signatures obtained from Rs1emR209C mice and patient-derived retinal organoids using the multiomics approach provide opportunities to unravel potential therapeutic targets for incurable retinal diseases, such as XLRS.

The invention provides an application of an ELABELA derived peptide in preparation of a medicine for treating renal fibrosis. The ELABELA derived peptide is an ELABELA polypeptide of which the C terminal is amidated. Through screening, it is found that the amidated Elabela derived peptide has the effect of inhibiting renal fibrosis on the cellular level and the animal level, endoplasmic reticulum stress and cell aging in the renal fibrosis development process can be relieved, the amidated Elabela derived peptide can be used for preparing drugs for treating or relieving renal fibrosis diseases, and the amidated Elabela derived peptide has a large clinical transformation application prospect.

The invention belongs to the technical field of bioelectricity medicine and cell therapy, and particularly relates to a system for carrying out intracellular electric treatment based on ultrahigh-frequency ultrashort pulses and application of the system. The system comprises a high-voltage ultrashort pulse generation module, a control module and an adherent cell processing module, wherein the high-voltage ultrashort pulse generation module is respectively connected with the control module and the adherent cell processing module; the control module presets a plurality of pulse parameter combinations, the high-voltage ultrashort pulse generation module outputs a pulse electric field with preset parameters according to an instruction sent by the control module, and the pulse electric field acts on an organelle through the adherent cell processing module. High-frequency ultrashort pulses are adopted, the unique electric field distribution characteristic of the high-frequency ultrashort pulses can penetrate through cell membranes without causing permanent damage, and the high-frequency ultrashort pulses directly act on specific organelles such as mitochondria, endoplasmic reticulum and lysosome. And the system can flexibly induce four main programmed cell death modes, is suitable for different treatment requirements, and is beneficial to precise medical treatment.

The invention discloses a cascade targeting ruthenium (II) complex as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, preparing a ligand I; step 2, preparing a ligand 2; and step 3, preparing a cascade targeting ruthenium (II) complex: preparing a metal ruthenium complex through the ligand I, and carrying out coordination on the metal ruthenium complex and the ligand II to obtain the cascade targeting ruthenium (II) complex. The cascade targeting ruthenium (II) complex is novel in structure, has tumor targeting and endoplasmic reticulum targeting properties, can be used as a photosensitizer, and can generate active oxygen under illumination for photodynamic therapy of tumors. The target cascade targeting type ruthenium (II) complex is applied to preparation of anti-cancer drugs and preparation of type II ICD inducer drugs. The compound not only has excellent anti-tumor activity, but also can be used as a type II ICD inducer to induce tumor cells to generate ICD; the preparation yield is high; the repeatability is good and the like; good biocompatibility is achieved, and clinical use is facilitated.

According to the selective organ targeting endoplasmic omentum fusion nano delivery platform and the application thereof, the platform realizes bidirectional regulation and control of organ targeting selectivity and cell membrane fusion efficiency through reasonable design of components and proportions on the basis of the synergistic effect of functional lipid combination, and the delivery efficiency of the endoplasmic omentum fusion nano delivery platform is improved. The problems that the organ specificity accumulation of a traditional delivery system for delivering drugs is insufficient, the release efficiency of intracellular drugs after delivery is low and the accumulation of organelles is limited are solved, and the drugs are delivered to specific organs in a targeted manner and are highly fused with endoplasmic reticulum membranes of cells in the organs; the method can be widely applied to the treatment of organ specificity and endoplasmic reticulum related diseases, such as lung intracellular bacterial infection treatment, pulmonary fibrosis treatment, anti-tumor vaccine construction and the like. The raw materials for construction of the platform are easy to obtain, and the platform is prepared by adopting a scalable simple process and has a good clinical transformation prospect.

The invention belongs to the technical field of biological medicines, and particularly discloses a pork liver source / photosensitizer-loaded pork liver source lipid nanoparticle and application thereof in endoplasmic reticulum stress enhanced phototherapy. The lipid extraction method provided by the invention has the advantages of mature process, low cost, easily available raw materials, clear components of the obtained lipid, and high biocompatibility. The pork liver source lipid nanoparticles prepared from natural lipid extracted from pork liver have good endoplasmic reticulum targeting property, and can be used as a carrier to efficiently load a photosensitizer IR780; the related nanoparticle preparation method is simple, good in repeatability and easy for expanded production. A phototherapy system constructed by the nanoparticles can generate active oxygen in situ in an endoplasmic reticulum under near-infrared laser irradiation to initiate strong endoplasmic reticulum stress, so that the anti-tumor effect of photothermal and photodynamic combined treatment is remarkably enhanced, and the nanoparticles can be widely applied to efficient and accurate treatment of various solid tumors.

The application discloses a kind of nanoclusters with golgi targeting and its preparation method and application, the nanocluster is zinc sulfide nanocluster, the surface of the zinc sulfide nanocluster is coupled with targeting ligand, the targeting ligand can actively target RA synovial activated macrophage, and can target golgi subcellular organelle, the nanocluster can release H2S gas in inflammatory weak acid microenvironment.The application can realize "inflammatory joint enrichment-> macrophage active uptake-> lysosome escape-> golgi deep anchoring" multi-level precise delivery, release H2S gas in inflammatory weak acid microenvironment, fundamentally reshape intracellular Ca 2+ Steady state, relieve endoplasmic reticulum, golgi and mitochondria triple stress, block inflammatory signal pathway, realize the efficient, safe treatment of RA.

The invention discloses a diabetic nephropathy marker PARP1, a primer combination and application, and relates to the technical field of biology. The sequence of the primer combination is PARP1-F, and is as shown in SEQ ID NO. 1; 1, PARP1-R as shown in SEQ ID NO. 2; an internal reference primer beta-actin-F, which is as shown in SEQ ID NO. 17; an internal reference primer beta-actin-R is as shown in SEQ ID NO. 18. The invention finds that the expression of PARP1 in the kidney tissue of a patient with diabetic nephropathy is increased and is positively correlated with ACR (Activated Receptor). The invention also finds that PARP1 is related to podocyte damage, and knocking down PARP1 can relieve podocyte endoplasmic reticulum stress and apoptosis, and can reflect the progress of DKD to a certain extent.

The present invention relates to a composition for protecting cells from endoplasmic reticulum (ER) stress and oxidative stress induced by ultrafine dust, comprising galangin as an active ingredient. Galangin, which is an active ingredient of the composition for cell protection of the present invention, alleviated an increase in reactive oxygen species, intracellular calcium, and ER stress-related proteins induced by ultrafine dust PM2.5. In addition, galangin counteracted PM2.5-induced cell cycle arrest at the G0 / G1 phase and restored cell cycle regulators and matrix metalloproteinases. As a result, galangin can enhance cell protection against PM2.5-induced ER stress and cell cycle arrest via reactive oxygen species. Therefore, it is suggested that the composition comprising galangin as an active ingredient of the present invention has great potential as a candidate for a preventive agent or a therapeutic agent for diseases related to ER stress and oxidative stress induced by PM2.5.

The invention relates to the technical field of biological medicine, and provides application of a dental pulp stem cell exosome in preparation of a medicine for treating and preventing osteoarthritis, a preparation method of the dental pulp stem cell exosome comprises the following steps: S1, obtaining dental pulp tissue from a dental pulp cavity of an in-vitro tooth; s2, pretreating dental pulp tissues, adding digestive juice, digesting, centrifuging, discarding supernatant, adding a complete culture medium containing antibiotics into precipitates, mixing, filtering and centrifuging to obtain dental pulp stem cells; and S3, culturing the dental pulp stem cells by using a complete culture medium, when the confluence degree of the dental pulp stem cells reaches 60-80%, replacing the serum-free culture medium for continuous culture, collecting the dental pulp stem cell conditioned culture medium, and performing differential centrifugation to obtain the dental pulp stem cell exosome. According to the technical scheme, the regulating effect of the dental pulp stem cell exosome on endoplasmic reticulum stress and the mechanism of the dental pulp stem cell exosome for improving cartilage cell degeneration are explored.

The invention discloses a preparation method and application of an intelligent nano regulator for tumor photoimmunotherapy, and belongs to the technical field of fine chemical engineering. The intelligent nano regulator is formed by assembling an amphiphilic polymer modified by an immunologic adjuvant Resiquimod (R848) and biotin and a small molecule photosensitizer Try5-Ph-3F together. The nano regulator can be effectively taken by cancer cells and positioned in endoplasmic reticulum, and has good tumor targeting ability. Under illumination, the photosensitizer generates a large amount of reactive oxygen species (ROS) to cause endoplasmic reticulum aggression so as to promote release of immune injury related molecular patterns (DAMPs) and promote maturation of dendritic cells (DCs), in addition, R848 is released in a tumor slightly acidic environment, and maturation of DCs is promoted. And the two drugs cooperate to promote the outbreak of immune response, so that the host obtains stronger and longer-time anti-tumor ability.

The invention relates to the field of biological medicine, in particular to an endoplasmic reticulum targeting peptide, an agonist, a preparation method and application. Wherein the agonist comprises an endoplasmic reticulum targeting peptide, and further comprises a mitochondrial membrane disturbing sequence and a sequence with cathepsin B response, the mitochondrial membrane disturbing sequence is KLAKLAK 2, and the sequence with cathepsin B response is GFLG; the endoplasmic reticulum targeting peptide comprises a KKKAA peptide sequence, and chlorin e6 and R8 peptide are connected to the KKKAA peptide sequence; the mitochondrial membrane disturbing sequence is connected to the R8 peptide through a sequence with cathepsin B response. According to the scheme, the characteristics of biochemical information interaction and tumor growth regulation of the mitochondria and the endoplasmic reticulum are utilized, excessive stress of the endoplasmic reticulum and a mitochondria calcium uptake channel are activated at the same time in a safe and controllable mode, free Ca < 2 + > in the endoplasmic reticulum rapidly flows into the mitochondria, the steady states of the endoplasmic reticulum and the mitochondria of tumor cells are damaged, and the tumor growth is controlled. And the two synergistically induce cells to start an apoptosis procedure, so that the aim of treating diseases is fulfilled.