72 results about "Amorphous calcium phosphate" patented technology

The invention provides an antibacterial synergy-mineralization regulation difunctional nano material as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: mixing a disodium hydrogen phosphate solution and a calcium chloride solution to obtain an amorphous calcium phosphate suspension; mixing the amorphous calcium phosphate suspension with a tannic acid solution to obtain an ACP-TA suspension; and finally, mixing the ACP (at) TA suspension and the hydroxypropyl trimethyl ammonium chloride chitosan solution to obtain the bifunctional nano material. A cross-linked shell is formed through the electrostatic adsorption effect of tannic acid and hydroxypropyl trimethyl ammonium chloride chitosan of the antibacterial layer, amorphous calcium phosphate is effectively wrapped, the crystallization process of amorphous calcium phosphate can be remarkably delayed, long-acting release of calcium and phosphorus ions is ensured, the antibacterial function can be effectively achieved, and the service life of the coating is prolonged. An ideal local microenvironment is provided for remineralization of tooth hard tissue, and finally biomimetic mineralization of enamel and deep mineralization plugging of dentinal tubules are achieved.

The present invention relates to improved phosphopeptide stabilised amorphous calcium phosphate and / or amorphous calcium fluoride phosphate complexes and compositions containing those complexes. Methods of making the complexes of the invention and of treatment dental hypersensitivity are also provided. In one embodiment, the invention provides a stannous-associated phosphopeptide (PP) stabilized amorphous calcium phosphate (ACP) or amorphous calcium fluoride phosphate (ACFP) complex having a stannous ion content of equal to, or greater than, 1 mole of stannous per mole of PP but less than 4 moles of stannous per mole of PP.

The present invention relates to improved complexes of amorphous calcium phosphate and / or calcium by amorphous fluoride phosphate stabilised phosphopeptides / phosphoproteins by addition of stannous ions. These complexes have anticariogenic properties useful to protect tooth structures as they remineralize (repair) early stages of dental caries and have other dental / medical applications (including anti-calculus, anti-erosion / corrosion and anti-dentinal hypersensitivity). Methods of making the complexes of the invention and of treatment or prevention of various dental conditions including dental caries, dental calculus, dental erosion / corrosion and dental hypersensitivity are also provided.

Provided is a novel biomarker for determining the onset of urolithiasis. This biomarker for determining the onset of urolithiasis is characterized by comprising at least one among calcium phosphate crystals and amorphous calcium phosphate in urine.

The invention belongs to the technical field of biomedical materials, and particularly relates to a mesoporous silica functional filler and a preparation method thereof, and a composite resin material and a preparation method and application thereof. The mesoporous silica functional filler provided by the invention has remineralization performance and pH response type. Functional modification is carried out on the surface of mesoporous silica, the modified mesoporous silica can load phosphorylated chitosan / amorphous calcium phosphate through electrostatic attraction, and the pH response performance is given to the modified mesoporous silica. The phosphorylated chitosan can simulate the effect of dentin matrix protein 1, can regulate the remineralization process of dentin, and can chelate calcium ions to stabilize amorphous calcium phosphate in the solution to form an amorphous nano precursor. The polyelectrolyte may be replaced with a biomimetic analog to initiate the remineralization process. The size of the mineral precursor and the deposition sequence and layer of the mineral precursor inside and outside the collagenous fiber can be regulated and controlled, and meanwhile nucleation and growth of apatite crystals inside and outside the collagenous fiber can be promoted.

The present invention relates to a silk fibroin mineral composite material synthesized by induction of a silk fibroin water-soluble segment as a template and a preparation method thereof, wherein the silk fibroin mineral composite material is obtained by inducing the orderly growth of calcium ions and hydrogen phosphate ions by the silk fibroin water-soluble segment, and is a bulk mineral formed by the uniform distribution of amorphous calcium phosphate of silk fibroin and amorphous calcium phosphate growing along the c-axis, or rod-shaped calcium hydrogen phosphate crystals juxtaposed and aggregated, and the surface of the bulk mineral is coated with silk fibroin. The silk fibroin mineral composite material provided by the present invention has a unique oriented structure, has a large potential application value in the preparation of bone tissue repair materials, can be induced by the induction of the silk fibroin water-soluble segment, add a phosphorus source and a calcium source, induce the directional growth of minerals at the wound, and realize the rapid regeneration and repair of bone tissue.

This invention discloses a composite hydrogel based on a polysaccharide-based hybrid dual network, its preparation method, and its applications, belonging to the field of marine polysaccharide technology. Based on the important role of amorphous calcium phosphate, a precursor in biomineral formation, in mineralization, this invention prepares a multifunctional composite hydrogel with wet bonding, antibacterial, and remineralization functions. This composite hydrogel can achieve rapid and convenient repair of enamel demineralization. This invention prepares oxidized sodium alginate via an alcohol-aqueous phase using the sodium periodate method. Sodium alginate reacts with carboxymethyl chitosan and dopamine through a Schiff base reaction to form dynamic imine bonds, thus preparing an in-situ self-crosslinking hydrogel. Simultaneously, amorphous calcium phosphate is introduced for secondary crosslinking, preparing a dense dual-network composite hydrogel. The composite hydrogel prepared by this invention has an interconnected three-dimensional porous structure, stable mechanical properties, and uniformly dispersed amorphous calcium phosphate in the form of nanospheres. The composite hydrogel prepared by this invention provides an integrated strategy with clinical translational potential for orthodontic enamel demineralization, with broad market prospects.

This invention discloses a method for reducing the stability of amorphous calcium phosphate. The method involves adding an additive solution to an ACP solution; the additive is a calcium- and phosphorus-free inorganic salt that does not react with calcium ions and orthophosphate ions to form a precipitation reaction. This invention, by adding an inorganic salt after the formation of amorphous calcium phosphate, primarily increases the ionic strength of the solution, reduces calcium and phosphorus activity, promotes the dissolution of amorphous calcium phosphate, thereby altering its structure, significantly reducing its stability, and accelerating the formation of hydroxyapatite. Furthermore, this method uses low-cost raw materials, contributing to cost reduction.

The invention discloses a preparation method and application of a citric acid-based polymer stable amorphous calcium phosphate porous scaffold for bone defect repair. The preparation method of the citric acid-based polymer stable amorphous calcium phosphate porous scaffold comprises the following steps: carrying out a polymerization reaction on an amorphous calcium phosphate precursor and citric acid-based poly (1, 8-octylene glycol-citrate) at 80 DEG C for 2-3 days or carrying out a polymerization reaction at 80 DEG C for 3 days and then carrying out a reaction at 120 DEG C for 1 day to obtain the porous scaffold with stable amorphous calcium phosphate characteristics. According to the present invention, the uniform dispersion and the effective and stable combination of the amorphous calcium phosphate precursor particles in the citric acid-based poly (1, 8-octylene glycol-citrate) matrix are achieved, and the biomimetic mineralized bone repair scaffold with characteristics of porous structure, high mechanical strength, controllable degradability and excellent osteogenic activity is successfully constructed. According to the stent, the technical problem of insufficient stability in application of amorphous calcium phosphate is remarkably solved, the osteogenic activity of the material is improved, and the stent has a great application prospect in the field of clinical bone defect repair.

The application provides an anti-friction and corrosion-resistant medical magnesium alloy coating, a preparation method thereof and a medical magnesium alloy. From the surface of the medical magnesium alloy substrate to the outside, the medical magnesium alloy coating comprises a bottom coating and a surface coating which are arranged in sequence; the bottom coating comprises magnesium hydroxide; and the surface coating comprises nano-scale amorphous calcium phosphate. The application uses the magnesium alloy as the substrate, converts the surface layer of the magnesium alloy into a dense magnesium hydroxide layer through an alkali-thermal reaction, and then generates a continuous and dense calcium phosphate coating on the surface of the bottom coating through a hydrothermal reaction to form a composite coating. The composite coating has good biocompatibility and can be completely degraded. In addition, the coating can not only alleviate the problem of too fast degradation of the magnesium alloy, but also has high wear resistance and excellent bonding strength, thereby providing sustained protection for magnesium alloy fixing products.

The invention relates to a 3D-printed bone repair scaffold material with double functions and a preparation method and application thereof, polycaprolactone, nano barium titanate and citrate-modified amorphous calcium phosphate are uniformly mixed, heated for reaction and ultrasonically dispersed to prepare biological printing ink, and the bone repair scaffold material is obtained through 3D printing. The bone repair scaffold material prepared by the invention has shape memory and piezoelectricity, is good in biocompatibility, and can promote osteogenic differentiation and bone defect repair.

A composition for treating dentin hypersensitivity, comprising: a first component comprising: (i) a first rheology modifying polymer; (ii) a calcium ion source; (iii) a positively charged basic amino acid; and (iv) an acidifying agent, wherein the first component has a pH in the range of about 2.0 to about 7.0; a second component comprising: (i) a second rheology modifying polymer; (ii) a phosphate ion source; and (iii) an alkalizing agent, wherein the second component has a pH in the range of about 7.0 to about 14.0; wherein the first component and the second component are combined into a mixture such that a cationic amorphous calcium phosphate gel composition is created instantly when the pH of the mixture reaches a pH range of about 7.0 to about 9.5.

The present invention relates to the technical field of enamel restoration, and particularly to a method for biomineralizing enamel with amorphous calcium phosphate nanoclusters stabilized by fluorine and gallic acid. The reagent combination includes a gallic acid-calcium phosphate-fluoride solution, and the raw materials of the gallic acid-calcium phosphate-fluoride solution include gallic acid, a calcium ion source, a phosphorus ion source, and a fluoride ion source; the molar ratio of calcium element in the calcium ion source to gallic acid is 0.01-0.5, and the molar ratio of calcium element in the calcium ion source to phosphorus element in the phosphorus ion source is 0.1-5. This technical solution can solve the technical problems in the prior art of lacking an effective and non-toxic method and system for promoting the stability of calcium phosphate nanoclusters to achieve enamel biomineralization. By using gallic acid and fluoride ions to co-biomineralize enamel, calcium phosphate nanoclusters for biomineralizing dentin can be prepared to achieve effective mineralization repair. Gallic acid is an inexpensive and edible compound, and this solution has an ideal application and promotion prospect.

The invention provides injectable mineralized hydrogel microspheres as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the invention, methacrylic anhydride gelatin, methacrylic anhydride sodium alginate and bone cell membrane vesicle blended hydrogel microspheres are prepared through gas shearing microfluidic and photopolymerization technologies, and mineralized hydrogel microspheres are prepared through in-situ pre-mineralization. The compression modulus of the mineralized hydrogel microspheres is obviously enhanced, and amorphous calcium phosphate particles are formed in the gel fibers in situ. The mineralized hydrogel microspheres can effectively promote osteogenic differentiation of BMSCs, and cells adhered to the surfaces of the microspheres can express bone cell markers more quickly. In addition, the material has good blood vessel formation regulating and controlling capacity, formation of a mature bone cell network can be accelerated in an in-vivo environment, and bone defect healing is accelerated. Therefore, the mineralized hydrogel microspheres can promote intelligent bone regeneration by accelerating bone cell network reconstruction.

The invention relates to a carboxymethyl chitosan-amorphous calcium phosphate composite anti-caries material as well as a preparation method and application thereof, the composite anti-caries material is a composite gel system and comprises carboxymethyl chitosan, amorphous calcium phosphate and glycerol, and hydroxyapatite nanowires can be selectively added into the carboxymethyl chitosan-amorphous calcium phosphate composite anti-caries material. The composite anti-caries material provided by the invention successfully solves the ubiquitous problems of the existing fluoride-free remineralization material that the mineralization efficiency needs to be improved, the clinical operability is poor and the like, and provides a novel fluoride-free scheme which is safe, efficient and convenient to use for prevention and treatment of early caries.

The invention relates to a mineralized protein-based bionic bone repair material as well as a preparation method and application thereof. Ca < 2 + > and PO4 < 3-> are introduced into the silk fibroin solution for a mineralization reaction, so that the amorphous calcium phosphate is directionally deposited on the silk fibroin molecular template, and a silk fibroin-amorphous calcium phosphate nano-composite is generated; after the mineralization reaction is finished, a mineralized product is centrifuged, frozen and freeze-dried, and the bionic bone repair material based on the mineralized protein is obtained. The bionic block material which is compact in structure and excellent in mechanical property can be constructed through high-pressure compression molding at normal temperature. The biomimetic bone repair material based on mineralized protein or a biomimetic block material further prepared on the basis of the scheme of the application shows excellent mechanical properties and structural stability in dry and wet environments, and also has good processability; the method is suitable for personalized bone defect repair and manufacturing of clinical orthopedic implantation instruments such as bone nails, bone plates and interbody fusion cages, and has wide application prospects and transformation values.

The invention discloses a biomimetic polypeptide cp20k-p3 for inducing remineralization of demineralized dentin and application of the biomimetic polypeptide cp20k-p3, the biomimetic polypeptide disclosed by the invention is a polypeptide cp20k-p3 derived from barnacle gum protein 20K, and the amino acid sequence of the biomimetic polypeptide cp20k-p3 is CNQKHPCWRRHGKKHGLHRKFHGNACNC. According to the bionic polypeptide disclosed by the invention, amorphous calcium phosphate can be rapidly generated in simulated body fluid and can be well adsorbed on the surface of a demineralized dentin matrix, and newly generated hydroxyapatite crystals can deeply seal exposed demineralized dentin tubules. A brand new biomineralization polypeptide is provided for bone demineralization dentin repair.

The present invention relates to improved phosphopeptide stabilised amorphous calcium phosphate and / or amorphous calcium fluoride phosphate complexes and compositions containing those complexes. Methods of making the complexes of the invention and of treating or preventing oral conditions are also provided. In one aspect, the present invention provides a composition comprising equal to or greater than about 8% w / v stannous associated phosphopeptide (PP) stabilized amorphous calcium phosphate (ACP) or amorphous calcium fluoride phosphate (ACFP) complexes wherein the complexes have a stannous ion content of equal to, or greater than, 1 mole of stannous per mole of PP but less than 4 moles of stannous per mole of PP.

The present invention provides a casein micelle composition containing amorphous calcium phosphate (CaP), wherein the casein micelles contain at least one calcium-sensitive casein and do not contain κ-casein. The present invention also provides a method for producing the casein micelle composition. The present invention also provides a food product containing the casein micelle composition.

The present invention relates to improved complexes of amorphous calcium phosphate and / or amorphous calcium fluoride phosphate stabilised by phosphopeptides / phosphoproteins by addition of stannous ions. These complexes have anticariogenic properties useful to protect tooth structures as they remineralize (repair) early stages of dental caries and have other dental / medical applications (including anti-calculus, anti-erosion / corrosion and anti-dentinal hypersensitivity). Methods of making the complexes of the invention and of treatment or prevention of various dental conditions including dental caries, dental calculus, dental erosion / corrosion and dental hypersensitivity are also provided.

The application discloses a kind of micro bone-blood vessel symbiosis based on gene activation and its preparation method and application.The system includes: the amorphous calcium phosphate nanoparticles of L-arginine, bovine serum albumin and alendronate sodium co-modified loaded BMP-2 mRNA;Methacrylic acid gel hydrogel microspheres encapsulating the nanoparticle;And E7 peptide grafted on the surface of hydrogel microspheres.It is captured by E7 peptide bone marrow mesenchymal stem cells, and delivers BMP-2 mRNA to activate intracellular BMP-2 and endothelial-type nitric oxide synthase expression, while using L-arginine to drive in-situ synthesis of nitric oxide, to synergistically enhance stem cell osteogenic differentiation and promote vascular sprouting ability, realize the vascularization synchronous regeneration of bone defect.The system solves the problem of unstable exogenous nitric oxide donor and bone-vessel regeneration decoupling, and has excellent biocompatibility and bone repair efficiency.

The invention relates to composite gel for remineralization of dog enamel as well as a preparation method and application of the composite gel. The composite gel comprises 5-15 wt% of casein phosphopeptide-amorphous calcium phosphate and 3-10 wt% of nano bioactive glass, and has a porous cluster structure, and the molar ratio of Ca to P is (1.8-2): 1. According to the preparation method, an in-situ coprecipitation process is adopted, and a compound with a uniform structure is formed by controlling the stirring rate and the solution dripping rate. The composite gel is combined with diode laser for use, ion migration and mineral conversion can be promoted, and synergistic interaction is achieved. According to the invention, the problems of low remineralization efficiency and limited mechanical property improvement in the prior art are solved, and the enamel mineralization structure and mechanical property can be effectively recovered on the premise of not damaging tooth tissues.

The application provides a preparation method of a collagen in-situ mineralized scaffold simulating a bone matrix, S1: extraction of type I collagen fibers; S2: preparation of a biomimetic mineralization solution: carboxymethyl chitosan (CMC) is placed in deionized water, after stirring and dissolving, K2HPO4 is added, CaCl2.2H2O is added drop by drop, and is fully dissolved and mixed to form a carboxymethyl chitosan / amorphous calcium phosphate biomimetic mineralization solution (CMC / ACP); S3: preparation of a biomimetic collagen scaffold composed of a simulated mineralized bone matrix: the acid-recombined collagen extracted in step S1 is fully mixed with hyaluronic acid (HA) and is placed in a dialysis bag, an external PBS buffer is used to gradually increase the pH value, after self-assembly for a period of time, the CMC / ACP biomimetic mineralization solution prepared in step S2 is added in the dialysis bag and is fully mixed, after mineralization for a period of time, the content of the dialysis bag is centrifuged to remove supernatant, and is repeatedly washed with deionized water, finally, after centrifugation, the precipitate is frozen overnight, and the biomimetic scaffold is finally obtained through freeze-drying. The application provides the preparation method of the collagen in-situ mineralized scaffold simulating the bone matrix.

This invention relates to the field of biomaterials technology, providing a composite material for biomimetic repair of dental hard tissues, its preparation method, and its application. The preparation method of the composite material includes the following steps: mixing a carboxybetaine-phosphonic acid copolymer with a calcium salt to obtain solution A; adding an aqueous phosphate solution to solution A to carry out an in-situ reaction to obtain the composite material for biomimetic repair of dental hard tissues. In the composite material provided by this application, the carboxybetaine-phosphonic acid copolymer, under the action of calcium phosphate ions, can form an amelogenin-like nanoribbon structure, simultaneously driving amorphous calcium phosphate to undergo supramolecular self-assembly, constructing a composite material conforming to the "self-assembly-mineralization" synergistic mode. This composite material can directionally deposit dense enamel-like enamel pillar structures of hydroxyapatite crystals on the surface of demineralized enamel, efficiently inducing oriented structural regeneration of damaged enamel and significantly restoring the mechanical properties of demineralized enamel.

The application discloses a preparation method and application of a temperature-sensitive injectable plastic mineralization material, and the material can be stored for a long time and is used for inducing tooth mineralization. By combining amorphous calcium phosphate with an amphiphilic surfactant, dispersion and stability of the amorphous calcium phosphate are promoted. The mineralization material obtained by loading synthetic and dried amorphous calcium phosphate particles on the amphiphilic surfactant is in a solid state and cannot be plasticized at room temperature, can be injected and plasticized when heated to a certain temperature, and has a better mineralization effect compared with the dried amorphous calcium phosphate particles. The solid bulk material at room temperature is beneficial to packaging, storage and use. When coated on teeth in the oral cavity, the material gradually melts and dissolves at the normal oral cavity temperature, spontaneously forms high-concentration micelle-loaded amorphous calcium phosphate nanoparticles in a solution on the tooth surface, and plays a mineralization role.

Disclosed are a low-crystalline calcium phosphate-based bone graft material and a method of preparing same, the bone graft material comprising at least one of dents and pores having an average diameter of 100 to 500 μm, having a BET specific surface area of 100 m2 / g or more, comprising both crystalline apatite and amorphous calcium phosphate, and having a degree of crystallization of 25% or less.

The invention relates to a hot gutta-percha composite filling material and a preparation method thereof, and relates to the technical field of biological medicine, the main technical scheme is that the preparation method of the hot gutta-percha composite filling material comprises the following steps: step 1), dissolving and mixing amorphous phosphate and a thermoplastic polymer in a volatile organic solvent to obtain a mixed solution; obtaining a mixture; then volatilizing the volatile solvent in the mixture to obtain a composite raw material; wherein the amorphous phosphate comprises amorphous calcium phosphate and / or copper-doped amorphous calcium phosphate; and 2) preparing the composite raw material into the hot gutta-percha composite filling material with the required specification. Amorphous calcium phosphate with high biological activity or a copper-doped amorphous calcium phosphate material is compounded in a gutta-percha point material, and further sealing can be realized through mineralization in a natural and humid dentin environment, so that the marginal adaptability of the gutta-percha point material to the wall of a root canal is enhanced.

A calcium phosphate-based core-shell structured material, a method for preparing the same, and an oral care composition using the same are provided. The calcium phosphate-based core-shell structured material includes an amorphous calcium phosphate (ACP) core and a β-tricalcium phosphate (β-TCP) shell covering the core. The method includes a first sintering step and a second sintering step. The first sintering step is to sinter an ACP material at between 700° C. and 800° C. to obtain an α-TCP shell. The second sintering step allows the α-TCP shell to form into the β-TCP shell by sintering at between 800° C. and 900° C. The oral care composition includes a calcium phosphate mixture and an orally receivable carrier. The calcium phosphate mixture includes a powder of the calcium phosphate-based core-shell structured material and a tricalcium phosphate powder mixed in a weight ratio from 3:5 to 3:7.

This invention discloses a 3D-printed scaffold with improved bioactivity and in vitro mineralization capacity, its preparation method, and its applications. The scaffold is prepared by layer-by-layer extrusion 3D printing using an ink comprising biphasic calcium phosphate (composed of octacalcium phosphate (OCP) and amorphous calcium phosphate (ACP)), sodium alginate, and a dispersant. This invention uses a mixture of OCP and ACP to form biphasic calcium phosphate (BCP) as the inorganic phase component of the 3D composite scaffold, and sodium alginate (SA) as the organic phase component. Simultaneously, the organic substance sodium alginate (SA) can simulate a blood clot as a temporary matrix, providing mechanical stability and traction for cells migrating from adjacent tissues. Furthermore, it provides a stable environment for new bone replacement during the gradual degradation of the scaffold by cells, offering significant advantages for subsequent scaffold implantation, attracting cell migration, and inducing mineralization.