11 results about "Calcium phosphate coating" patented technology

The invention relates to a curcumin-loaded MMP response type melittin nano-pellicle vesicle as well as a preparation method and application thereof, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing curcumin entrapped lipidosome; then carrying out culture amplification on engineering bacteria carrying melittin recombinant plasmids, extracting cell membranes after removing cell walls, and carrying out ultrasonic treatment and membrane extrusion to obtain melittin cell membrane nano-vesicles; fusing the curcumin lipidosome and the cell membrane nano-vesicles in an ultrasonic extrusion mode to obtain fused vesicles; and finally, carrying out surface calcium phosphate mineralization treatment on the fused vesicles to obtain the curcumin-loaded MMP response type melittin nano pellicle vesicles. The nano mycofilm vesicle prepared by the invention can be used for preparing antitumor drugs, and the biocompatibility and in-vivo stability of nanoparticles are improved by introducing a calcium phosphate coating; through combined delivery of melittin and curcumin, the anti-tumor effect is enhanced, so that the growth and proliferation of tumor cells are inhibited.

The invention belongs to the technical field of biomedicine and nano crossing, and particularly relates to a biomineralization-based nucleic acid aptamer functionalized DNA nano-structure compound as well as a preparation method and application thereof. The compound has a tetrahedral structure and comprises a functional DNA tetrahedral framework and a calcium phosphate coating coated on the surface of the functional DNA tetrahedral framework, the functionalized DNA tetrahedral framework is formed by self-assembly of four DNA single chains; wherein the 5'end of one DNA single chain is modified with a nucleic acid aptamer. The bionic calcium coating is used as a protective shell, so that the in-vivo stability and the angiogenesis promoting activity of the Apt02 are remarkably improved. Meanwhile, by virtue of the specific spatial configuration of the tetrahedral DNA nanostructure, the compound can release calcium ions after being taken by cells, so that osteogenic differentiation is further promoted, and a new strategy for synergistic promotion is provided for vascularized bone regeneration.

The present disclosure relates to a calcium phosphate coating that includes a plurality of nanometer sized calcium phosphate crystals that form a single layer. The coating is on a surface of a conductive substrate and is about 1 to 1000 nanometers thick, and greater than 25 percent of the coated surface area comprises the single layer of the crystals.

The invention belongs to the technical field of medical prosthetic materials, and particularly relates to a zirconia-based medical prosthetic material and a preparation method and application thereof.The preparation method comprises the steps that S1, pretreatment is conducted, specifically, a zirconia preform is subjected to primary high-temperature sintering, then grinding, polishing and primary cleaning are conducted, and then secondary high-temperature sintering and secondary cleaning are conducted; s2, preparation of a surface drug-loading structure: preparing a drug-loading honeycomb porous nano-structure on the surface of the zirconium oxide preform by adopting phosphorylation treatment; s3, loading of metal ions: putting the zirconium oxide material into loading liquid containing the metal ions, reacting in a shaking table at 50-90 DEG C for 5-8 hours, taking out after the reaction is finished, washing and drying to obtain the zirconium oxide-based medical prosthesis material, and drying the zirconium oxide-based medical prosthesis material to obtain the zirconium oxide-based medical prosthesis material. The zirconia-based medical prosthetic material provided by the invention not only avoids the problems of poor wear resistance of PLGA, poor combination of a calcium phosphate coating and color of a dopamine coating, but also realizes slow release of metal ions, thereby promoting osseointegration more effectively.

The application provides an anti-friction and corrosion-resistant medical magnesium alloy coating, a preparation method thereof and a medical magnesium alloy. From the surface of the medical magnesium alloy substrate to the outside, the medical magnesium alloy coating comprises a bottom coating and a surface coating which are arranged in sequence; the bottom coating comprises magnesium hydroxide; and the surface coating comprises nano-scale amorphous calcium phosphate. The application uses the magnesium alloy as the substrate, converts the surface layer of the magnesium alloy into a dense magnesium hydroxide layer through an alkali-thermal reaction, and then generates a continuous and dense calcium phosphate coating on the surface of the bottom coating through a hydrothermal reaction to form a composite coating. The composite coating has good biocompatibility and can be completely degraded. In addition, the coating can not only alleviate the problem of too fast degradation of the magnesium alloy, but also has high wear resistance and excellent bonding strength, thereby providing sustained protection for magnesium alloy fixing products.

The application discloses a preparation method of a foam calcium phosphate-based energy-absorbing coating for extreme dynamic load protection, and belongs to the technical field of energy-absorbing coatings. The method comprises the following steps: S1, polyurethane sponge is respectively immersed in anhydrous ethanol, a NaOH solution and a dopamine solution for treatment, and then freeze-drying is performed; S2, the prepared polyurethane sponge is immersed in a sodium alginate gel solution to construct a dried gradient gel coating precursor; S3, the dried gradient gel coating precursor is immersed in a calcium phosphate deposition slurry to obtain a foam calcium phosphate coating precursor through an electric field orientation deposition method; and S4, a foam calcium phosphate-based energy-absorbing coating is prepared through a gradient sintering method. The application further provides the foam calcium phosphate-based coating prepared by using the above preparation method and application thereof. The preparation method of the foam calcium phosphate-based energy-absorbing coating for extreme dynamic load protection solves the problems of weak interfacial bonding strength, poor energy-absorbing and shock-absorbing effect and inability to resist explosion shock waves of existing explosion-proof coating materials and aerogel materials in an extreme dynamic load environment.

The invention discloses a preparation method of a composite fireproof door core, and relates to the technical field of fireproof doors, and the preparation method comprises the following steps: at least carrying out calcium phosphate coating flame-retardant treatment on straw coarse crushed materials to obtain flame-retardant straws; the preparation method comprises the following steps: mixing sulphoaluminate cement, aluminate cement and fly ash according to a preset mass ratio to prepare cement-based powder; uniformly mixing flame-retardant straw, cement-based powder, expanded vermiculite and water, pouring hydrogen peroxide, and stirring to obtain a mixture; and pouring the mixture into a mold for self-foaming and curing, demolding, and curing at a preset temperature for at least 7 days to obtain the composite fireproof door core. The flame-retardant straw is obtained by at least carrying out calcium phosphate coating flame-retardant treatment on the straw coarse crushed material, and the flame-retardant straw is combined with the cement-based powder, the expanded vermiculite and the hydrogen peroxide foaming process, so that the composite fireproof door core with excellent fireproof and heat-insulating properties, high mechanical strength and light-weight characteristics is successfully prepared.

The invention belongs to the technical field of biological materials and tissue engineering, and particularly relates to a bioactive protein-loaded strontium-doped bionic calcium phosphate coating and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing a base material; s2, immersing the base material into a first solution containing a calcium source and a phosphate radical source, and forming an amorphous calcium phosphate seed layer on the surface of the base material in situ; and S3, transferring the base material into a second solution containing a calcium source, a phosphate radical source, a strontium source and bioactive protein, and carrying out biomimetic mineralization deposition to form the strontium-doped octacalcium phosphate composite coating. The whole preparation process is mild in condition, a common high-temperature, high-pressure and vacuum environment or an external electric field in the prior art does not need to be adopted, the biological activity of the bioactive protein can be better reserved, the prepared coating has a layered or nano-sheet bionic microstructure similar to natural bone minerals, the coating is endowed with extremely high biological activity, and the application range is wide. And the cell adhesion, proliferation and osseointegration process can be promoted.

The invention discloses a bioactive dental implant with a coating capable of promoting regeneration of surrounding tissues and a preparation method of the bioactive dental implant. The titanium implant of the bioactive dental implant is sequentially subjected to acid etching and alkali treatment, and the surface of the titanium implant is of a nano honeycomb network structure; the coating comprises multiple layers of biomimetic mineralized calcium phosphate coatings uniformly loaded with protein-loaded calcium phosphate nanoparticles; the protein-loaded calcium phosphate nanoparticles are loaded into the biomimetic mineralized calcium phosphate coating in a self-assembly manner in the nucleation and mineralization crystallization process of calcium phosphate. According to the invention, a multi-layer biomimetic mineralized calcium phosphate coating is constructed on the surface of the titanium implant through a biomimetic mineralization technology, protein-loaded calcium phosphate nanoparticles are uniformly assembled into the coating, the binding force between the coating and a titanium substrate is strong, and the release of protein in the protein-loaded calcium phosphate nanoparticles is matched with the regeneration stage of tissues around the implant, so that the biomimetic mineralized calcium phosphate coating has good biocompatibility. The activity loss of the active factors can be effectively avoided, the controllable release of the active factors is realized, the preparation process is simple and the cost is low.

The application provides a ready-to-use mesoporous mineralized silk fibroin microsphere and application thereof, and the mesoporous mineralized silk fibroin microsphere (referred to as mSilMA) is prepared by the following steps: preparing a SilMA hydrogel microsphere through a photo-crosslinking oil / water emulsion method, and then combining a biomimetic mineralization method and a critical point drying method. The silk fibroin material which is not easy to degrade and has good biocompatibility is selected as a carrier, so that the problems of easy loss and long-term retention of BMP-2 are solved; the biomimetic mineralization forms a calcium phosphate coating to realize efficient loading and controlled release of BMP-2; the critical point drying forms a mesoporous structure, which is beneficial to drug loading and release. The mSilMA is applied to loading and controlled release of BMP-2, and the protein loading efficiency is high and can be long-acting and low-dose released. The application has simple components, mild preparation conditions and easy acquisition, provides a new way for bone defect repair, and has a wide clinical application prospect.

The invention relates to the technical field of biological coating preparation, in particular to a calcium-phosphorus coating preparation method and system.The method comprises the steps that a to-be-treated base body in a coating preparation environment is pretreated, a pretreated base body is obtained, a coating base material set is used for obtaining calcium-phosphorus precursor slurry, and the calcium-phosphorus precursor slurry is prepared; the method comprises the following steps: carrying out laser melting on a pretreated matrix and calcium-phosphorus precursor slurry to obtain a molten calcium-phosphorus layer, carrying out cooling crystallization on the molten calcium-phosphorus layer to obtain a target coating, carrying out quality detection on the target coating to obtain a detection result, if the detection result is unqualified, obtaining an abnormal type, and carrying out adjustment preparation on the target coating based on the abnormal type. And if the detection result is qualified, taking the target coating as the qualified coating. According to the invention, the high efficiency and stability of preparation of the calcium-phosphorus coating can be improved.