109 results about "Hydrogel microspheres" patented technology

The invention discloses a hydrogel microsphere sorting method, an artificial organ preparation method, a system and a medium. The method comprises the following steps: acquiring a hydrogel microsphere image in the micro-fluidic chip; analyzing the hydrogel microsphere image through a neural network to obtain an analysis result; based on the analysis result, hydrogel microspheres wrapping the target cells are sorted out; the neural network comprises a feature extraction layer and a multi-scale joint output head, and the hydrogel microsphere image is processed by the multi-scale joint output head after being convolved by the feature extraction layer to obtain an analysis result. According to the method, the hydrogel microspheres wrapped with the target cells are accurately identified and sorted by using a droplet microfluidic technology, a standardized artificial organ can be prepared based on the sorted hydrogel microspheres, and the functional maturity and reliability of the artificial organ are remarkably improved.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a pH-responsive curcumin sustained-release microsphere hydrogel, a preparation method and application thereof in spinal cord injury repair materials. The preparation method is as follows: short fibers are prepared by pretreating and grinding discarded eggshell membranes; after sodium alginate is dissolved, the sodium alginate is blended with the eggshell membrane short fibers, curcumin is added, and stirring is uniformly performed to form a mixed solution; the mixed solution is injected into a calcium chloride solution by using a drip crosslinking method to form, and an eggshell membrane and sodium alginate curcumin microsphere hydrogel is obtained. The hydrogel has excellent water content, swelling performance, biocompatibility, blood compatibility and drug sustained-release function, and can be applied to spinal cord injury repair.

This invention relates to the field of microsphere manufacturing technology, and in particular to a device, method and application for preparing hydrogel microspheres for encapsulating organoids. This invention improves the preparation efficiency of hydrogel microspheres by changing the shape and spatial distribution of the high-voltage electrostatic field and controlling the size of hydrogel microspheres by voltage as a single variable. It also addresses the problems of large surface roughness of hydrogel microspheres prepared by traditional high-voltage electrostatic non-contact devices and the easy fibrosis of sodium alginate hydrogel microspheres during in vivo transplantation.

The invention relates to a preparation method of stepped cross-linked myofibril-pectin core-shell hydrogel microspheres, which comprises the following steps: preparing a myofibril protein dispersion liquid and a pectin solution by using a phosphate buffer solution containing NaCl as a solvent; preparing an anthocyanin stock solution by taking a phosphate buffer solution as a solvent; mixing the three components under an ice bath condition, and then reacting at 2-5 DEG C for 1-3 hours; 2) dropwise adding the reaction solution obtained in the step 2) into a continuously stirred CaCl cross-linking solution at 2-5 DEG C, standing and curing after dropwise adding, and washing with distilled water to obtain single-layer hydrogel microspheres with uniform particle size and smooth surface; and naturally draining the surface moisture of the single-layer hydrogel microspheres, soaking the single-layer hydrogel microspheres in a freshly prepared 1-4% pectin solution for 20-40 seconds, then taking out the single-layer hydrogel microspheres, transferring the single-layer hydrogel microspheres into another CaCl crosslinking solution, and continuing crosslinking for 10-20 minutes to form a compact shell structure. And after the reaction is completed, washing with distilled water. The core-shell hydrogel microspheres can be used for detecting volatile ammonia, monitoring and indicating pork freshness and the like.

The invention belongs to the technical field of microorganism immobilization, and particularly relates to a preparation method of hydrogel microspheres loaded with microorganisms to realize gas-liquid interface exposure. The hydrogel microsphere is of a core-shell structure and comprises core hydrogel containing nutrient substances, a polyelectrolyte layer located on a core-shell interface and an outer hydrogel shell used for immobilizing microorganisms. Stable construction and consistency control of the microsphere structure are realized in the core-shell assembly process; under the action of gaseous pollutant exposure and osmotic pressure, the outer-layer hydrogel shrinks and forms a film structure, so that microorganisms are stably distributed and are continuously in a gas-liquid interface exposure state; the core hydrogel continuously releases water and nutrient substances to the shell layer, the growth and biological functions of immobilized microorganisms under the semi-exposure condition are maintained, the polyelectrolyte layer limits the microorganisms from migrating to the core, and the stability of the exposed state of the microorganisms is guaranteed. The hydrogel microspheres have good optical transparency and microorganism loading consistency, and are suitable for in-situ detection of the toxic effect of gaseous pollutants.

The invention discloses an application and method of Tween 80 combined immobilization technology enhanced microorganisms in degradation of naphthalene pollution in water, the main body of the combined immobilization technology is SA / CS at Tween 80 hydrogel microspheres loaded with NM-1 flora, and the NM-1 flora is halotolerant bacteria obtained by enrichment and domestication from deep-sea sediments of a certain sea ditch. The SA / CS at Tween 80 microspheres are formed by crosslinking sodium alginate (SA), chitosan (CS) and Tween 80, hydroxyl functional groups of the Tween 80 and SA / CS are crosslinked to enhance the mechanical strength of the microspheres and regulate and control the pore structure to improve the mass transfer efficiency, hydrophobic-CH functional groups at the end positions of the Tween 80 strengthen the adsorption capacity to naphthalene, meanwhile, a co-crosslinking structure at the interface of the SA / CS at Tween 80 hydrogel microspheres forms a biochemical metabolic reaction buffer area, and the adsorption capacity to naphthalene is improved. The direct toxicity stress of the high-concentration naphthalene on the flora is effectively avoided, and the effective biological removal of the high-concentration naphthalene is realized. The SA / CS-coated Tween 80 hydrogel microspheres prepared by the invention can effectively maintain the efficient metabolic activity and degradation efficiency of the loaded NM-1 flora, and show excellent cycle stability and good engineering application potential.

The invention discloses a multifunctional Janus composite diaphragm, a preparation method and application, the diaphragm is formed by coating two sides of a Celgard diaphragm with different functional layers, one side is a Fe / Bi-S-C coating layer, the Fe / Bi-S-C coating layer obtains hydrogel microspheres through a sol-gel reaction of chitosan, dopamine and a metal salt precursor, the hydrogel microspheres are subjected to freeze drying, carbonization and sulfuration treatment, and the multifunctional Janus composite diaphragm is obtained. FeS and Bi2S3 nanoparticles are generated in situ in the carbon skeleton; and a stable Van der Waals heterojunction layer assembled by BN and MXene nanosheets is arranged on the other side of the Van der Waals heterojunction layer. According to the Janus composite diaphragm disclosed by the invention, through the synergistic strengthening effect of the functional layers coated on the two sides, the diaphragm not only can effectively inhibit the generation and growth of lithium dendrites, but also can efficiently capture and catalyze the conversion of polysulfides, so that the cycling stability and sulfur utilization rate of a battery are remarkably improved. The diaphragm realizes collaborative optimization in the aspects of mechanical stability, thermal safety and electrochemical performance.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

The invention discloses hydrogel microspheres capable of loading radioactive isotopes and a preparation method of the hydrogel microspheres. The microsphere is formed by copolymerizing a macrocyclic ligand structure, a hydrophilic monomer and a cross-linking agent, and a macrocyclic ligand is connected with a polymer main chain through a bridging structure containing p-aminobenzoic acid and aliphatic diamine and can efficiently and stably complex various radioactive isotopes. The microspheres have the characteristics of being close to water in density, good in suspension property, smooth in surface and rich in elasticity, and are easily and uniformly distributed in tumor peripheral blood vessels. The preparation method comprises the following two routes: firstly synthesizing the functional monomer and then copolymerizing, or firstly preparing the microspheres and then gradually grafting. The problems that existing microsphere nuclides are prone to falling off, high in density and poor in suspension property are effectively solved, and the microsphere is suitable for interventional radioactive embolism treatment.

The present application relates to the technical field of intervertebral disc degeneration treatment and drug delivery, and discloses an injectable oxynate sodium functionalized hydrogel microsphere loaded with TGFbeta3 and application, by loading glycolysis inhibitor SO in the GelMA microsphere matrix and post-adsorbing TGFbeta3, on the one hand, SO is used to inhibit the excessive active glycolysis in the degenerative microenvironment, reduce lactic acid accumulation and relieve inflammation, on the other hand, TGFbeta3 is used to effectively promote the synthesis of collagen and proteoglycan, both of which synergistically act from the two aspects of metabolic regulation and matrix synthesis, and jointly promote the recovery of intervertebral disc homeostasis, therefore, the oxynate sodium functionalized microsphere loaded with TGFbeta3 constructed by the present application can realize the synergistic treatment of multiple targets and multiple pathways by synchronously delivering metabolic regulators and synthesis promoters, so as to more effectively delay or reverse the intervertebral disc degeneration process, and finally achieve the beneficial effect of the double repair effect of simultaneously regulating abnormal glycolysis metabolism of degenerative nucleus pulposus cells and promoting extracellular matrix synthesis.

The present application relates to the technical field of biological medicine, and particularly relates to a responsive self-assembled polypeptide hydrogel microsphere system, a preparation method and application thereof. The responsive self-assembled polypeptide hydrogel microsphere system comprises GelMA, polypeptide I, an anti-inflammatory active ingredient and a polypeptide hydrogel, the polypeptide I is grafted with the GelMA, the anti-inflammatory active ingredient is loaded on the GelMA to form a GEL+polypeptide I+anti-inflammatory active ingredient microsphere, and the polypeptide hydrogel is used to wrap the GEL+polypeptide I+anti-inflammatory active ingredient microsphere to form the responsive self-assembled polypeptide hydrogel microsphere system. The polypeptide hydrogel as a carrier in the microsphere system is a smart responsive hydrogel, a MMP-2 specific recognition polypeptide sequence is introduced through a molecular engineering technology, and a functionalized GEL+polypeptide I+anti-inflammatory active ingredient microsphere is compounded. The above research results indicate that the responsive self-assembled polypeptide hydrogel microsphere system provided by the present application is expected to be widely applied in anti-inflammatory and / or tissue repair.

The invention discloses an environmental DNA tracer for hydrological survey and pollutant traceability and a tracing method, and belongs to the technical field of environmental monitoring. The tracer agent comprises a carrier matrix and free DNA encapsulated in the carrier matrix, and the carrier matrix is selected from one of silicon dioxide microspheres and sodium alginate-chitosan composite hydrogel microspheres according to drainage basin water diversion tracing scene requirements; the free DNA is a specific double-stranded DNA fragment which is artificially designed and synthesized, and meets the uniqueness and detectability of the sequence. The tracer agent is environmentally friendly, high in specificity and high in sensitivity, the tracing method is easy and convenient to operate, the water flow migration track and the pollutant diffusion range can be accurately analyzed, and the tracer agent is suitable for tracing monitoring under the complex hydrological condition.

The invention provides a silica sand water permeable brick and a preparation process thereof. The silica sand water permeable brick sequentially comprises a surface layer, a glue layer and a bottom layer from top to bottom, the surface layer is prepared from the following raw materials in parts by weight: 50 to 60 parts of coarse silica sand, 20 to 30 parts of medium-fine silica sand, 12 to 18 parts of metakaolin-based geopolymer, 10 to 15 parts of elastic particles, 3 to 8 parts of temperature-sensitive hydrogel microspheres, 0.1 to 0.3 part of polypropylene fiber, 0.3 to 0.8 part of water repellent agent and 4 to 7 parts of water; the adhesive layer is prepared from the following raw materials in parts by weight: 90 to 100 parts of metakaolin-based geopolymer, 5 to 10 parts of polymer modifier, 0.1 to 0.3 part of hydroxypropyl methyl cellulose and 25 to 35 parts of water, and the bottom layer is prepared from the following raw materials in parts by weight: 75 to 80 parts of coarse aggregate, 15 to 20 parts of cement, 5 to 10 parts of elastic particles, 1 to 3 parts of silica fume, 0.2 to 0.5 part of water reducing agent and 4.5 to 6.5 parts of water. The temperature-sensitive hydrogel microspheres and the elastic particles supplement each other to form an efficient cleaning chain of vibration loosening and discharging, pollution of pollutants is effectively resisted, and the water permeable brick can still keep excellent water permeability after being used for a long time.

A nanozyme-loaded nucleus pulposus matrix hydrogel microsphere is provided. A simple-to-prepare LOX—MnO2 nanozyme is provided, and the simple-to-prepare LOX—MnO2 nanozyme is loaded onto the GDNP, and an LOX—MnO2-loaded and glucose-enriched decellularized nucleus pulposus hydrogel microsphere, namely the nanozyme-loaded nucleus pulposus matrix hydrogel microsphere is formed through a new two-stage temperature-controlling microfluidic system.

Porous hydrogel microspheres and the preparation method are provided thereof, the method comprising: mixing a gelation phase material and a cell-loaded pore-forming phase material at a temperature of 5-15° C. using a droplet microfluidic chip, and forming first droplets through a shear force; the cell-loaded pore-forming phase material includes a pore-forming phase material and cells, the gelation phase material including gelatin methacryloyl, polyethylene glycol diacrylate, and a photoinitiator, and the pore-forming phase material including polyethylene oxide and gelatin; then shearing a droplet stream formed by the first droplets through an oil phase material to form second droplets; curing the second droplets by an ultraviolet light irradiation to form hydrogel microspheres, and removing the pore-forming phase material from the hydrogel microspheres to obtain porous hydrogel microspheres. The porous hydrogel microspheres with stable morphology can be prepared, and the porous structure of the porous hydrogel microspheres is conducive to cell proliferation and expansion.

This application relates to the field of biomedical engineering, specifically disclosing a stem cell preparation for treating acute ischemic stroke and its preparation method. The preparation comprises a carrier core formed integrally through membrane fusion and an engineered stem cell membrane shell. The carrier core is a homologous stem cell exosome loaded with reactive oxygen species (ROS) scavenging nanoenzymes and can anchor responsive hydrogel microspheres encapsulating neurotrophic factors. The engineered shell covers the core, and its surface is covalently linked to brain-targeting molecules and thermosensitive lipid molecules, the latter of which can expose the target molecules at body temperature for intelligent navigation. The preparation method includes stepwise preparation of the drug-loaded core and functionalized shell, followed by programmed freeze-thaw treatment to induce membrane fusion of the two into a complete preparation. The product of this application can efficiently target ischemic lesions and achieve sequential synergistic treatment by first scavenging ROS and then releasing trophic factors. Its preparation method has advantages such as modularity, mild conditions, and good preservation of bioactivity.

The invention relates to the technical field of biomedical materials, and discloses chitosan-based hydrogel microspheres with high water-retaining property as well as a preparation method and application of the chitosan-based hydrogel microspheres. In order to solve the problems of poor water retention capacity and insufficient mechanical strength of the existing chitosan hydrogel, the invention constructs a hydrogel microsphere with three network cross-linked structures. The triple network cross-linked structure is jointly constructed by a dual cross-linked network consisting of glycidyl methacrylate modified chitosan and lipoic acid modified chitosan, and a nano reinforcing material uniformly dispersed in the dual cross-linked network. The preparation method comprises the following steps: firstly preparing two kinds of modified chitosan, namely glycidyl methacrylate and lipoic acid, then mixing the two kinds of modified chitosan with a nano reinforcing material and the like to obtain a precursor solution, and finally preparing the microspheres through a reverse emulsion method and a photocuring technology. By constructing a unique triple network, the water-retaining property and the mechanical strength of the hydrogel microspheres are remarkably improved.

The invention discloses hydrogel microspheres embedded with multi-morphology gold nanoparticles as well as a preparation method and application of the hydrogel microspheres, and belongs to the technical field of spectral analysis. The hydrogel microsphere comprises a hydrogel carrier and gold nanoparticles embedded in the hydrogel carrier. The preparation method comprises the following steps: preparing multi-morphology gold nanoparticles by adopting a gold seed growth method; mixing gold nanoparticles and a sodium alginate solution to prepare a precursor; and enabling the precursor to form micron-sized liquid drops by using a high-pressure injection / electric atomization technology, and injecting the micron-sized liquid drops into a calcium chloride solution for cross-linking and curing to obtain the hydrogel microspheres embedded with the multi-morphology gold nanoparticles. When the hydrogel microspheres containing the target object are prepared, gold nanoparticles, sodium alginate and the target object are mixed to prepare a precursor, and then the hydrogel microspheres containing the target object are further prepared according to the method. The hydrogel microsphere is stable in structure, good in dispersity and high in environmental adaptability, can overcome the defects that gold nanoparticle colloid is prone to agglomeration and sensitive to environmental conditions, and meanwhile has excellent SERS enhancement efficiency.

Provided in the present application is a preparation method for starch hydrogel microspheres, belonging to the technical field of embolic microsphere manufacturing. The preparation method for starch hydrogel microspheres comprises the following steps: adding a first crosslinking agent to a starch solution and mixing to obtain an aqueous phase solution; adding an oil phase solution to the aqueous phase solution and mixing to obtain a mixed liquid; and adding a second crosslinking agent to the mixed liquid and mixing for reaction to obtain the starch hydrogel microspheres. The starch hydrogel microspheres prepared by the method feature such advantages as long in-vivo degradation time and adjustable degradation time, and can be applied to a wide range of disease types.

The present application relates to a kind of seedling substrate and its preparation method, it relates to crop planting technical field, a kind of seedling substrate, including hydrogel microspheres and composite substrate component, the composite substrate component includes: peat, vermiculite, perlite, humic acid, zeolite powder, slow-release fertilizer particle;The composition of the hydrogel microspheres includes: soybean protein isolate, sodium carboxymethyl cellulose, naphthalene acetic acid, xanthan gum, genipin, chitosan, calcium chloride, bacillus subtilis bacterial agent;Its preparation process includes hydrogel microspheres preparation, premixing, initial mixing, final mixing step;The seedling substrate and its preparation method protected in the present application, utilize the porous loose structure formed by hydrogel microspheres, this porous structure makes microsphere only slightly expand after absorbing water, while guaranteeing water-retention capacity, guaranteeing ventilation performance, provide stable microenvironment for seedling growth, further improve crop seedling quality.

This invention provides a stress-triggered selective targeting hydrogel microsphere, its preparation method, and its application, belonging to the field of biomedical technology. The core of this invention is the "stress-triggered charge reversal" mechanism. It utilizes the interaction between weakly cross-linked disulfide bonds broken under stress and "charge reversal" messenger molecules to achieve "charge reversal" of nanoparticles under stress. A dual-network hydrogel microsphere system is prepared using microfluidic technology to achieve bearing lubrication and stress relaxation within the joint cavity. Stress-triggered charge reversal is achieved at stress-overloaded cartilage lesions, imparting a positive charge to the drug-loaded nanoparticles within the hydrogel microspheres. This significantly enhances the ability of the drug-loaded nanoparticles to penetrate deep into the cartilage matrix and target chondrocytes for drug release under the guidance of the charge. This enables the selective and targeted release of appropriate drug concentrations to deep-seated chondrocytes in pathological microenvironments induced by different stress intensities, achieving highly effective and low-toxicity treatment of osteoarthritis.

This invention relates to the field of water treatment technology, specifically to a method for adsorbing heavy metals in copper-containing wastewater. The method includes: preparing a modified chitosan composite material; preparing a composite fiber filter membrane; preparing composite hydrogel microspheres; and treating the copper-containing wastewater. This invention first dissolves chitosan and mixes it with nano-ferric oxide to form a physical coating, imparting magnetic responsiveness to the chitosan. Then, it modifies the chitosan using 1,3,5-phenyltricarboxylic acid and triethylenetetramine. A three-dimensional network cross-linked structure is constructed by reacting glutaraldehyde with the amino groups of chitosan and triethylenetetramine to produce modified chitosan. Finally, graphene oxide is loaded onto the surface and pores of the modified chitosan through impregnation and drying to obtain a modified chitosan composite material. When this material is added to pretreated wastewater, it can significantly improve the adsorption capacity for copper ions in copper-containing wastewater.

This invention discloses a wear-resistant clothing fabric and its preparation method, belonging to the field of textile technology. It comprises, from the outside in, a wear-resistant surface layer, a microcapsule self-healing layer, a main functional film layer, a salt-retaining buffer layer, and a moisture-wicking inner layer, sequentially connected by dotted hot-pressing composite bonding. The wear-resistant surface layer is a double-layer asymmetric fabric woven from gradient functional core-spun yarns of copolymerized modified nylon 66, island-type composite fibers, and carbon nanotube composite nylon 66 fibers. The microcapsule self-healing layer is a polyurethane film with microcapsules capable of dynamic covalent bond exchange and self-healing. This invention achieves long-term UV resistance through copolymerized modified fibers, utilizes hydrogel microspheres to retain and release chloride ions, actively repairs damage with the microcapsule self-healing layer and dynamic covalent bond materials, and eliminates galvanic corrosion using all-plastic accessories and a conductive network, effectively slowing down the performance degradation of the fabric in high-salt-spray environments.

The application discloses a hydrogel microsphere for adjusting a senescent microenvironment and a preparation method and application thereof, wherein the hydrogel microsphere is obtained by mixing hollow mesoporous silica IL37@HMS loaded with recombinant protein IL37 and nanoparticles Man-4OI-PLGA modified by mannose and loaded with 4OI when preparing a pH-responsive hydrogel microsphere. The hydrogel can dissociate in an acidic microenvironment of degenerative tissue and release the carried drugs, can effectively protect the biological activity of IL37, realize slow release of the drugs, significantly improve the residence time and treatment efficiency of IL37 at a lesion site, can effectively protect the stability of itaconic acid in the body, and realize precise release of the itaconic acid at the lesion site, and greatly improve the enrichment efficiency of the itaconic acid in macrophages. The hydrogel meets the special requirements of small space, high pressure, mechanical strength, biocompatibility and controllable drug release of an intervertebral disc, and has important significance for treatment of degenerative diseases.

The invention discloses a graded pore structure microsphere based on catalytic foaming as well as a preparation method and application of the graded pore structure microsphere. Liquid drops with core-shell structures are obtained by utilizing a three-phase double-emulsion microfluidic device; hydrogen peroxide / methacryloyl collagen, yeast / methacryloyl collagen / methacrylate hyaluronic acid and vegetable oil are added into an inner phase, an intermediate phase and an outer phase of a microfluidic device respectively, and core-shell droplets can be obtained to serve as a micro-droplet reactor for catalytic foaming reaction. The foamed liquid drops are subjected to light curing, and hydrogel microspheres with bubble cavities are obtained; then, yeast wall-breaking enzyme and lauryl sodium sulfate are used for treatment to remove cell structures of yeast, freeze drying is carried out, hydrogel microspheres with hierarchical pore structures are obtained, finally, polydopamine is deposited on the surfaces of the hierarchical pore microspheres for functionalization, and the hydrogel microspheres are obtained.

The invention relates to an embolism microsphere with a photothermal effect and a preparation method and application thereof, and belongs to the technical field of embolism microspheres. The preparation method comprises the following steps: mixing starch and a cross-linking agent in water to obtain an aqueous phase solution; adding the water-phase solution into the oil-phase solution, and carrying out reversed-phase suspension polymerization reaction to obtain starch hydrogel microspheres; wherein the cross-linking agent comprises at least one of sodium trimetaphosphate or sodium tripolyphosphate; oxidizing the starch hydrogel microspheres with sodium periodate to obtain oxidized starch microspheres; the oxidized starch microspheres are subjected to surface modification by adopting dopamine, a polydopamine coating layer is formed on the surfaces of the oxidized starch microspheres, and the embolism microspheres with the photothermal effect are obtained. The embolism microspheres have degradability and drug loading performance at the same time, embolism-chemotherapy-photothermal triple therapy is cooperated, drug release can be controlled through the photothermal effect, and therefore the embolism microspheres can be effectively used for TAE or TACE treatment.

The present invention belongs to the technical field of hydrogels. Disclosed are hydrogel microspheres, a preparation method therefor, and use thereof. According to the present invention, epigallocatechin gallate and vitamin E are self-assembled into a hydrophobic polyphenol-based micelle, wherein the hydrophobic polyphenol-based micelle, with EGCG as the core, forms a stable structure by means of non-covalent interactions (such as hydrophobic interactions, hydrogen bonding, and π-π stacking), thereby enhancing the stability and functionality of the hydrophobic core. Then, the hydrophobic structure is mixed with hydrophilic sodium alginate to form a gel, ultimately resulting in hydrogel microspheres with a unique amphiphilic heterogeneous structure. The hydrogel microspheres can exist stably in an aqueous environment, and actively capture dietary fats, thereby improving the ability to capture dietary fats, and improving the stability of the hydrogel microspheres in the gastrointestinal tract.

The present application relates to a kind of based on double-chamber hydrogel microsphere intestinal bionic organ model and its construction method, belong to biotechnology field.It is specifically double-chamber hydrogel microsphere with rough pit surface wrapped by intestinal barrier, construction method includes the following steps: the preparation of double-chamber hydrogel microsphere microfluidic device, preparation of rough double-chamber hydrogel microsphere with rough surface and load content, microsphere surface matrix glue modification and surface construction intestinal barrier.It is specifically applied to drug testing and the application research of microorganism and intestinal interaction.The intestinal bionic organ model prepared in the present application has simple manufacturing steps, short manufacturing time, low cost, and the subsequent culture maturation time is greatly shortened compared to other organoid models.The intestinal organ microsphere is expected to become a research platform for intestinal diseases, to promote the research of various intestinal disease treatment methods, and to help cope with the treatment challenges brought by various intestinal complex diseases.

The application discloses a microfluidic device for magnetic functionalized cell hydrogel microsphere fixation and cryopreservation, and belongs to the technical field of cell cryopreservation. The microfluidic device comprises a substrate layer provided with a cavity with a recess, the bottom of the cavity is provided with a receiving part, and a magnetic part is arranged in the receiving part; according to the microfluid flow direction, the microfluid channel layer comprises two inlet channels, a serpentine mixing channel, a perfusion chamber and an outlet channel which are sequentially communicated; the bottom of the perfusion chamber is provided with a plurality of recesses, and the recesses are used for fixing the magnetic functionalized cell hydrogel microspheres to be cryopreserved; and the microfluidic device further comprises a top layer and a sealing cover used for sealing the perfusion chamber. The microfluidic device can well realize the fixation and cryopreservation of the magnetic functionalized cell hydrogel microspheres, and can conveniently realize the loading or removal of cells, improve the operation convenience and the cell survival rate.

The disclosure provides synthetic cytotoxic cells, comprising a hydrogel microsphere and a TNF-related apoptosis-inducing ligand (TRAIL), or active fragment thereof. In aspects, the TRAIL or active fragment thereof is attached to the surface of the hydrogel microsphere. The disclosure further provides methods of preparing the synthetic cytotoxic cells and methods of use thereof in the treatment of cancer.