196 results about "Hydrogel microspheres" patented technology

The invention relates to an organ-like microsphere embedding slicing method. The method specifically comprises the following steps: 1) preparing hydrogel microspheres and dyeing the hydrogel microspheres with fuchsin; (2) dehydrating the dyed hydrogel microspheres and the organoid together, then putting the dehydrated hydrogel microspheres and the organoid into an embedding mold, adding an embedding agent for embedding, and obtaining an embedded sample after the embedding agent is solidified; and 3) slicing the embedded sample, and receiving the sliced sample by using a glass slide. According to the method, the hydrogel microspheres are firstly dyed, and the dyed hydrogel microspheres and the organoid are embedded together, so that the peripheral area of the organoid can be obviously red, and the magenta dyeing area on the section sample can be clearly observed even after the section sample is sliced. The hydrogel microspheres dyed by fuchsin can be used as positioning marks of organoid-containing microspheres, can provide obvious prompts in the early transparent dehydration process and the later embedding section process, and effectively help an operator to judge the section repairing degree and the section collecting degree.

The invention belongs to the technical field related to tumors, and discloses a preparation method of self-developing embolism microspheres. Comprising the following steps: producing calcium alginate microspheres through cross-linking, obtaining polyelectrolyte hydrogel microspheres through electrostatic complexation, and obtaining the self-developing embolism microspheres through amino cross-linking. According to the invention, sodium alginate and polyamino acid are used as raw materials for preparation, so that the preservative is safe and non-toxic and has biodegradability, and the degradation product has no toxic or side effect on human body. The microspheres are prepared by adopting an electrostatic spraying method, organic solvents are not used, and the microspheres are green and environment-friendly and have no toxic residues.

The invention relates to a heparinized gelatin / sodium alginate pancreas islet packaging material with an anti-fibrosis function as well as a preparation method and application of the heparinized gelatin / sodium alginate pancreas islet packaging material. The preparation method comprises the following steps: adding gelatin into normal saline, soaking, performing autoclaved sterilization to obtain a gelatin solution, then adding epsilon-polylysine hydrochloride into the gelatin solution, and finally adding glutamine transaminase for reaction to obtain an epsilon-polylysine grafted gelatin solution; adding heparin into the solution, and reacting to obtain a heparinized gelatin solution; adding sodium alginate and glutamine transaminase, reacting, diluting, preparing hydrogel microspheres by adopting an electrostatic spraying method, and cross-linking to obtain the hydrogel microspheres. According to the invention, heparin molecules are incorporated into a pancreas islet packaging system, so that fibrosis at the periphery of the microspheres can be relieved, the pancreas islet cells are packaged by using the pancreas islet packaging material to prepare the biological artificial pancreas, the pancreas islet cell proliferation and insulin secretion can be promoted, and the pancreas islet packaging material has a good diabetes treatment effect.

This invention belongs to the field of biopharmaceutical technology and specifically discloses size-controlled bacteriophage hydrogel microspheres and their preparation method. The hydrogel microspheres are composed of bacteriophage, a polymer carrier, and a curing agent and are prepared using electrostatic spray technology. Compared with existing hydrogel microsphere preparation methods, these hydrogel microspheres can significantly prolong the sustained release time of bacteriophage in the intestinal environment and improve their stability in the gastric environment. Furthermore, by adjusting the preparation parameters, the size of the microspheres can be flexibly controlled to meet different application scenarios and needs.

The invention discloses a functional hydrogel microsphere and a preparation method and application thereof, and relates to the technical field of medicines.The preparation method comprises the steps that a hyaluronic acid methacrylic acid precursor solution and an IL-1beta shRNA adenovirus stock solution are mixed to obtain an Ad-atHAMA precursor solution; preparing the Ad (at) HAMA precursor solution through an oil-in-water micro-fluidic technology to obtain an Ad (at) HAMA microsphere; and sequentially adding the Ad (at) HAMA microspheres, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide into a 2-morpholinoethanesulfonic acid buffer solution, carrying out activating treatment, adding E selectin, and incubating to obtain the functionalized hydrogel microspheres. The functionalized hydrogel microspheres can inhibit a related mechanism of acute spinal cord injury pathological progress and promote neural function repair by targeting adsorption of neutrophil to regulate Nets-triggered innate immune inflammation cascade.

The invention belongs to the technical field of scale inhibitors, and particularly relates to a slow-release scale inhibitor and a preparation method thereof.The preparation method comprises the steps that 3, 4, 5-allyl trihydroxybenzoate, itaconic acid, sodium hydrogen sulfite, methacryloyloxyethyl-n-hexadecyl-dimethyl ammonium bromide and deionized water are mixed, after water bath heating, an ammonium persulfate aqueous solution is slowly added, and the scale inhibitor is obtained after reaction; the preparation method comprises the following steps: dissolving agar in deionized water, adding polyethylene glycol modified sodium alginate and a scale inhibitor into the deionized water, stirring, dropwise adding into edible oil to obtain gel microspheres, adding the gel microspheres into a calcium chloride aqueous solution, slowly stirring, cross-linking, cleaning and drying to obtain the slow-release scale inhibitor. The hydrogel microspheres swell to slowly release the scale inhibitor, meanwhile, the released scale inhibitor is hydrolyzed in water, ester groups are slowly broken, polycarboxylic acid, 3, 4, 5-trihydroxybenzoic acid and hydroxyethyl-n-hexadecyl-dimethyl ammonium bromide are slowly released, and good scale inhibition and corrosion inhibition effects are achieved.

The invention discloses a hydrogel microsphere sorting method, an artificial organ preparation method, a system and a medium. The method comprises the following steps: acquiring a hydrogel microsphere image in the micro-fluidic chip; analyzing the hydrogel microsphere image through a neural network to obtain an analysis result; based on the analysis result, hydrogel microspheres wrapping the target cells are sorted out; the neural network comprises a feature extraction layer and a multi-scale joint output head, and the hydrogel microsphere image is processed by the multi-scale joint output head after being convolved by the feature extraction layer to obtain an analysis result. According to the method, the hydrogel microspheres wrapped with the target cells are accurately identified and sorted by using a droplet microfluidic technology, a standardized artificial organ can be prepared based on the sorted hydrogel microspheres, and the functional maturity and reliability of the artificial organ are remarkably improved.

The invention relates to core-shell hydrogel microsphere preparation equipment which comprises a microsphere preparation device and a microsphere collection device, the microsphere preparation device is provided with a nuclear phase inlet, a shell phase inlet, a plurality of nuclear phase distribution runners communicated with the nuclear phase inlet, and a plurality of shell phase distribution runners communicated with the shell phase inlet; the nuclear phase distribution flow channels and the shell phase distribution flow channels are converged into coaxial flow channels at the tail ends in a one-to-one correspondence manner; the microsphere collecting device comprises a microsphere collecting pool, the microsphere collecting pool is arranged below the coaxial flow channel, and the microsphere collecting pool is used for containing oil phase fluid; the nuclear phase fluid entering from the nuclear phase inlet and the shell phase fluid entering from the shell phase inlet form layered fluid at the coaxial flow channel, and the layered fluid is dropped into the microsphere collecting pool. According to the core-shell hydrogel microsphere preparation equipment provided by the invention, high-flux preparation of core-shell hydrogel microspheres can be realized.

The invention discloses a periodontal local drug delivery system with antioxidant, anti-inflammatory and antibacterial synergistic effects and a preparation method of the periodontal local drug delivery system, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing an organic phase from DSPC, cholesterol, SA and resveratrol, and preparing a cationic liposome by taking PBS as a water phase; then preparing a water phase from AlgMA and LAP, taking a surfactant solution as an oil phase, and preparing polydopamine hydrogel microspheres through PDA surface modification; and finally, carrying out physical adsorption loading. Through the synergistic effect of the resveratrol, the cationic liposome and the polydopamine, a periodontitis pathological chain is comprehensively intervened, synergistic treatment is achieved, excellent antibacterial performance, anti-inflammatory effect and antioxidant effect are achieved, the retention rate of the medicine is increased, and the problems that traditional treatment medicine cannot achieve antibacterial, inflammation regulation and oxidative stress synergistic treatment at the same time, and the treatment effect is poor are solved. The residence time of the medicine in the periodontal pocket is short, and frequent administration is needed.

The application discloses a preparation method of a high-activity multi-level-pore boronic acid imprinting hydrogel microsphere adsorbent and application of the adsorbent to selective adsorption of a flavonoid compound naringin (NRG). The application enhances surface functionalization of COFs by dopamine modification, prepares thiol functionalized covalent organic framework microspheres, synthesizes thiol microspheres by using an emulsion interfacial polymerization method, prepares COFs boron affinity imprinting microsphere adsorbents based on boron affinity, and applies the adsorbents to selective adsorption of NRG in agricultural wastewater. The prepared adsorbent has a significant multi-layer adsorption effect, and the adsorption capacity is significantly improved by using boron affinity. The multi-level-pore structure of the COFs microspheres provides abundant sites for adsorption, and significantly improves the mass transfer rate. In addition, the prepared adsorbent has significant specificity in adsorption and separation of NRG, and provides a promising adsorption material for industrialized separation of NRG.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a pH-responsive curcumin sustained-release microsphere hydrogel, a preparation method and application thereof in spinal cord injury repair materials. The preparation method is as follows: short fibers are prepared by pretreating and grinding discarded eggshell membranes; after sodium alginate is dissolved, the sodium alginate is blended with the eggshell membrane short fibers, curcumin is added, and stirring is uniformly performed to form a mixed solution; the mixed solution is injected into a calcium chloride solution by using a drip crosslinking method to form, and an eggshell membrane and sodium alginate curcumin microsphere hydrogel is obtained. The hydrogel has excellent water content, swelling performance, biocompatibility, blood compatibility and drug sustained-release function, and can be applied to spinal cord injury repair.

The invention provides Cr (VI) ion imprinted biomass carbon aerogel microspheres and a preparation method and application thereof.The preparation method comprises the steps that a bubble-rich mixed solution of gelatin, chitosan, glacial acetic acid, sodium bicarbonate and lauryl sodium sulfate is dripped into alkali liquor, and Cr (VI) ion imprinted biomass hydrogel microspheres are obtained through a surface ion imprinting technology; and carrying out freeze drying and high-temperature carbonization to prepare the Cr (VI) ion imprinted biomass carbon aerogel microspheres. The inner part of the large-hole interconnected thin-wall structure is of a three-dimensional interpenetrating network structure. When the pH value is equal to 2, the Cr (VI) ion imprinting biomass carbon aerogel microsphere has a Cr (VI) ion removal rate of 99% or above, has a wide pH working range, and has a Cr (VI) ion removal rate of 70% or above when the pH value is equal to 1-5. In the presence of interfering ions, the selectivity coefficient K value of the Cr (VI) ion imprinted biomass carbon aerogel microspheres to Cr (VI) ions is far greater than 1, and the Cr (VI) ion imprinted biomass carbon aerogel microspheres have a relatively large relative selectivity coefficient K'value, and still show the highest adsorption capacity to the Cr (VI) ions in the coexistence of various heavy metal ions.

The invention relates to the technical field of microsphere manufacturing, in particular to a device and method for preparing hydrogel microspheres wrapping organoid and application, by changing the shape and spatial distribution of a high-voltage electrostatic field, the size of the hydrogel microspheres is controlled through a voltage single variable, the preparation efficiency of the hydrogel microspheres is improved, and the hydrogel microspheres are prepared. The problems that the hydrogel microspheres prepared by a traditional high-voltage static non-contact device are large in surface roughness and the sodium alginate hydrogel microspheres are prone to fibrosis during in-vivo transplantation are solved.

The present application relates to a kind of core-shell hydrogel microsphere preparation device.The core-shell hydrogel microsphere preparation device includes microfluidic chip and hydrophobic connecting pipe, the microfluidic chip is equipped with core phase flow channel, shell phase flow channel, water same axis flow channel, oil phase flow channel, ball forming cavity and outlet flow channel, the end of the core phase flow channel and the shell phase flow channel meet, the meeting point is connected with the first end of the water same axis flow channel, the water same axis flow channel and the end of the oil phase flow channel meet in the ball forming cavity, the ball forming cavity is communicated with the first end of the outlet flow channel, the inner diameter of the ball forming cavity is 0.2~2 mm, the inner diameter of the outlet flow channel is 0.4~2 mm, the outer diameter of the hydrophobic connecting pipe is matched with the inner diameter of the outlet flow channel, the inner diameter of the hydrophobic connecting pipe is less than or equal to the inner diameter of the ball forming cavity, one end of the hydrophobic connecting pipe is inserted into the outlet flow channel.

This invention relates to the field of microsphere manufacturing technology, and in particular to a device, method and application for preparing hydrogel microspheres for encapsulating organoids. This invention improves the preparation efficiency of hydrogel microspheres by changing the shape and spatial distribution of the high-voltage electrostatic field and controlling the size of hydrogel microspheres by voltage as a single variable. It also addresses the problems of large surface roughness of hydrogel microspheres prepared by traditional high-voltage electrostatic non-contact devices and the easy fibrosis of sodium alginate hydrogel microspheres during in vivo transplantation.

The invention discloses a lipidosome composite hydrogel microsphere for repairing damaged intestinal mucosa and a preparation method thereof, LHMs (at) 4-OI is composed of hyaluronic acid modified targeted drug-loaded lipidosome, sodium alginate, hydrochloric acid treated sucralfate and a CaCl cross-linking agent, and rapid gel forming is carried out under the voltage of 11.0 KV through an electrospray technology. The microspheres have pH responsiveness and gastric juice digestion resistance, CD44 receptor targeting is achieved through hyaluronic acid modification, sodium alginate and Ca form an egg box structure to provide a three-dimensional network, and sucralfate enhances adhesion to isolate harmful substances. The preparation method comprises the steps of preparation of the drug-loaded liposome by a film hydration method, hyaluronic acid modification and electrospray crosslinking, is simple to operate and low in cost, and can be used for large-scale production. The microspheres can effectively load an anti-inflammatory drug 4-OI, inhibit expression of inflammatory factors and promote self-repair of damaged intestinal mucosa, and have wide application prospects in the fields of abdominal infection, inflammatory bowel diseases, colorectal cancer and the like.

The present application discloses a Micro LED fluid assembly method based on microfluidics and dielectrophoresis, comprising the following steps: S1: making the Micro LED chip into Micro LED chip hydrogel microspheres; S2: suspending the Micro LED chip hydrogel microspheres in a liquid; S3: sorting out the Micro LED chip hydrogel microspheres that meet the performance requirements; S4: evenly distributing the Micro LED chip hydrogel microspheres on the target substrate, and then assembling them using dielectrophoresis traps; S5: the dielectrophoresis traps prepared on the target substrate successively capture the Micro LED chip hydrogel microspheres to form a large-scale ordered array; S6: releasing the Micro LED chip wrapped in the Micro LED chip hydrogel microspheres, which is the corresponding Micro LED chip array; the present application realizes the precise positioning and control of the Micro LED hydrogel microspheres, completes the high-precision transfer of the Micro LED chip, improves the transfer efficiency and positioning accuracy of the Micro LED, simplifies the Micro LED transfer process, reduces the production cost, and has broad market application prospects.

The invention relates to a preparation method of stepped cross-linked myofibril-pectin core-shell hydrogel microspheres, which comprises the following steps: preparing a myofibril protein dispersion liquid and a pectin solution by using a phosphate buffer solution containing NaCl as a solvent; preparing an anthocyanin stock solution by taking a phosphate buffer solution as a solvent; mixing the three components under an ice bath condition, and then reacting at 2-5 DEG C for 1-3 hours; 2) dropwise adding the reaction solution obtained in the step 2) into a continuously stirred CaCl cross-linking solution at 2-5 DEG C, standing and curing after dropwise adding, and washing with distilled water to obtain single-layer hydrogel microspheres with uniform particle size and smooth surface; and naturally draining the surface moisture of the single-layer hydrogel microspheres, soaking the single-layer hydrogel microspheres in a freshly prepared 1-4% pectin solution for 20-40 seconds, then taking out the single-layer hydrogel microspheres, transferring the single-layer hydrogel microspheres into another CaCl crosslinking solution, and continuing crosslinking for 10-20 minutes to form a compact shell structure. And after the reaction is completed, washing with distilled water. The core-shell hydrogel microspheres can be used for detecting volatile ammonia, monitoring and indicating pork freshness and the like.

The present invention provides a mechanoelectrically coupled hydrogel microsphere with stable mechanical properties and low-loss transduction, as well as a preparation method and application thereof, belonging to the technical field of tissue engineering biorepair materials. The present invention utilizes supramolecular engineering and microfluidics technology to introduce a slip-ring structured polyrotaxane and a conductive polypyrrole network to construct stress-electrically coupled hydrogel microspheres. The molecular slip mechanism of the slip-ring structure stores and releases mechanical energy, reducing mechanical losses, while the conjugated π-electron movement in the conductive network improves the efficiency of electron transfer inside the microspheres, thereby reducing the losses in stress-electric conversion for the first time. Compared with traditional piezoelectric hydrogel microspheres, the mechanoelectrically coupled hydrogel microspheres of the present invention have a stress-electric coupling efficiency of 2.3 times higher than that of traditional piezoelectric hydrogel microspheres, and the energy dissipation rate is reduced to 43%. The mechanoelectrically coupled hydrogel microspheres can alleviate cartilage damage in rats, improve behavioral outcomes, and promote the treatment of osteoarthritis by restoring low-loss transduction between tissues.

The invention belongs to the technical field of metal rust prevention, and discloses a preparation method and application of a humidity response release type hydrogel microsphere loaded with a vapor phase corrosion inhibitor. The preparation method comprises the following steps: dissolving chitosan / PVA in water and acetic acid according to a certain proportion, and adding a vapor phase corrosion inhibitor; dissolving span 80 in n-heptane to form an oil phase; stirring the two phases to form an emulsion; and carrying out cross-linking on glutaraldehyde, stirring, and carrying out centrifugal drying treatment, so as to prepare the microspheres loaded with the gas phase corrosion inhibitor. The preparation method is simple and high in operability and repeatability. According to the hydrogel microspheres prepared by the method, the loading capacity of the vapor phase corrosion inhibitor is high, and breathable release along with the change of environmental humidity can be realized; the BTA coated CS-PVA has high stability, and has good corrosion inhibition efficiency in an extreme environment.

The invention relates to a device for preparing core-shell hydrogel microspheres. The core-shell hydrogel microsphere preparation device comprises a microfluidic chip and a hydrophobic connecting pipe, the microfluidic chip is internally provided with a nuclear phase flow channel, a shell phase flow channel, a water phase axial flow channel, an oil phase flow channel, a balling cavity and an outlet flow channel, the tail ends of the nuclear phase flow channel and the shell phase flow channel are converged, the converging point is connected with the head end of the water phase axial flow channel, and the shell phase flow channel is connected with the shell phase flow channel. The tail end of the water phase axial flow channel and the tail end of the oil phase flow channel intersect in the balling cavity, the balling cavity is communicated with the head end of the outlet flow channel, the inner diameter of the balling cavity is 0.2-2 mm, the inner diameter of the outlet flow channel is 0.4-2 mm, the outer diameter of the drainage connecting pipe is matched with the inner diameter of the outlet flow channel, and the drainage connecting pipe is communicated with the head end of the water phase axial flow channel and the head end of the oil phase flow channel. The inner diameter of the drainage connecting pipe is smaller than or equal to that of the balling cavity, and one end of the drainage connecting pipe is inserted into the outlet runner.

The invention relates to the technical field of wastewater treatment, and discloses preparation and application of a MnFeOx / CSA three-dimensional particle electrode for antibiotic wastewater treatment.The preparation method comprises the steps that a metal organic framework (Mn-MOF) and sodium alginate are mixed according to the mass ratio of 1.0: 3.2 to prepare homogeneous gel; dropwise adding the gel into a ferrous sulfate solution with the concentration of 0.10 to 0.40 g.L <-1 >, and carrying out cross-linking to obtain hydrogel microspheres; and carrying out vacuum freeze drying and carbonization treatment at 750-850 DEG C in a nitrogen atmosphere to obtain the MnFeOx / CSA three-dimensional particle electrode. The electrode is used as a particle electrode to construct a three-dimensional electrode system, and the antibiotic-containing wastewater is subjected to electrochemical degradation in the presence of an external electric field and persulfate. According to the invention, through an in-situ loading strategy, catalyst agglomeration is effectively inhibited; and the constructed three-dimensional electrode system obviously improves the mass transfer efficiency and the degradation capability on antibiotics.

The invention belongs to the technical field of microorganism immobilization, and particularly relates to a preparation method of hydrogel microspheres loaded with microorganisms to realize gas-liquid interface exposure. The hydrogel microsphere is of a core-shell structure and comprises core hydrogel containing nutrient substances, a polyelectrolyte layer located on a core-shell interface and an outer hydrogel shell used for immobilizing microorganisms. Stable construction and consistency control of the microsphere structure are realized in the core-shell assembly process; under the action of gaseous pollutant exposure and osmotic pressure, the outer-layer hydrogel shrinks and forms a film structure, so that microorganisms are stably distributed and are continuously in a gas-liquid interface exposure state; the core hydrogel continuously releases water and nutrient substances to the shell layer, the growth and biological functions of immobilized microorganisms under the semi-exposure condition are maintained, the polyelectrolyte layer limits the microorganisms from migrating to the core, and the stability of the exposed state of the microorganisms is guaranteed. The hydrogel microspheres have good optical transparency and microorganism loading consistency, and are suitable for in-situ detection of the toxic effect of gaseous pollutants.

This invention discloses a method for constructing a three-dimensional in vitro blood-brain barrier model with barrier function and its application, belonging to the biomedical field. The method includes: preparing a light-controlled biofunctionalized hydrogel with methacrylamide gelatin as the main framework; preparing a suspension of neurovascular matrix components using astrocytes and neurons; uniformly mixing the light-controlled biofunctionalized hydrogel and the neurovascular matrix component suspension to obtain a homogeneous cell-hydrogel composite solution, and preparing it into three-dimensional hydrogel microspheres; transferring the three-dimensional hydrogel microspheres to the bottom of a Transwell culture dish insert to form a stable neurolateral matrix layer; and adding a suspension of brain microvascular endothelial cells into the lower chamber of the Transwell culture dish. This invention achieves the synergistic effect of multiple bioactive substances, overcoming the shortcomings of the single bioactive substance loading method in the prior art.

The invention discloses an application and method of Tween 80 combined immobilization technology enhanced microorganisms in degradation of naphthalene pollution in water, the main body of the combined immobilization technology is SA / CS at Tween 80 hydrogel microspheres loaded with NM-1 flora, and the NM-1 flora is halotolerant bacteria obtained by enrichment and domestication from deep-sea sediments of a certain sea ditch. The SA / CS at Tween 80 microspheres are formed by crosslinking sodium alginate (SA), chitosan (CS) and Tween 80, hydroxyl functional groups of the Tween 80 and SA / CS are crosslinked to enhance the mechanical strength of the microspheres and regulate and control the pore structure to improve the mass transfer efficiency, hydrophobic-CH functional groups at the end positions of the Tween 80 strengthen the adsorption capacity to naphthalene, meanwhile, a co-crosslinking structure at the interface of the SA / CS at Tween 80 hydrogel microspheres forms a biochemical metabolic reaction buffer area, and the adsorption capacity to naphthalene is improved. The direct toxicity stress of the high-concentration naphthalene on the flora is effectively avoided, and the effective biological removal of the high-concentration naphthalene is realized. The SA / CS-coated Tween 80 hydrogel microspheres prepared by the invention can effectively maintain the efficient metabolic activity and degradation efficiency of the loaded NM-1 flora, and show excellent cycle stability and good engineering application potential.

The invention discloses a multifunctional Janus composite diaphragm, a preparation method and application, the diaphragm is formed by coating two sides of a Celgard diaphragm with different functional layers, one side is a Fe / Bi-S-C coating layer, the Fe / Bi-S-C coating layer obtains hydrogel microspheres through a sol-gel reaction of chitosan, dopamine and a metal salt precursor, the hydrogel microspheres are subjected to freeze drying, carbonization and sulfuration treatment, and the multifunctional Janus composite diaphragm is obtained. FeS and Bi2S3 nanoparticles are generated in situ in the carbon skeleton; and a stable Van der Waals heterojunction layer assembled by BN and MXene nanosheets is arranged on the other side of the Van der Waals heterojunction layer. According to the Janus composite diaphragm disclosed by the invention, through the synergistic strengthening effect of the functional layers coated on the two sides, the diaphragm not only can effectively inhibit the generation and growth of lithium dendrites, but also can efficiently capture and catalyze the conversion of polysulfides, so that the cycling stability and sulfur utilization rate of a battery are remarkably improved. The diaphragm realizes collaborative optimization in the aspects of mechanical stability, thermal safety and electrochemical performance.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

The invention discloses hydrogel microspheres capable of loading radioactive isotopes and a preparation method of the hydrogel microspheres. The microsphere is formed by copolymerizing a macrocyclic ligand structure, a hydrophilic monomer and a cross-linking agent, and a macrocyclic ligand is connected with a polymer main chain through a bridging structure containing p-aminobenzoic acid and aliphatic diamine and can efficiently and stably complex various radioactive isotopes. The microspheres have the characteristics of being close to water in density, good in suspension property, smooth in surface and rich in elasticity, and are easily and uniformly distributed in tumor peripheral blood vessels. The preparation method comprises the following two routes: firstly synthesizing the functional monomer and then copolymerizing, or firstly preparing the microspheres and then gradually grafting. The problems that existing microsphere nuclides are prone to falling off, high in density and poor in suspension property are effectively solved, and the microsphere is suitable for interventional radioactive embolism treatment.

The application discloses a high-loading MXene-encapsulated magnetic porous microcapsule for adsorption and a preparation method and application thereof, and belongs to the field of biomedical materials, and the preparation method comprises the following steps: in step one, a hydrogel precursor solution doped with magnetic nanoparticles is used as an external phase, and a MXene dispersion liquid is used as an inner phase, and a microfluidic coaxial electrospraying device is used to generate monodisperse core-shell droplets with uniform sizes; in step two, the monodisperse core-shell droplets prepared in step one are subjected to freezing pretreatment, and then are crosslinked with divalent metal ions, so that double gelation of the core layer and the shell layer is triggered, and hydrogel microspheres with a core-shell structure are obtained; and in step three, the hydrogel microspheres with the core-shell structure obtained in step two are subjected to freeze drying, and high-loading MXene-encapsulated magnetic porous microcapsules are obtained. The application has excellent blood compatibility and high adsorption capacity, and is outstanding in removing uremic toxins including creatinine, urea and uric acid, and is a kind of adsorbent which is both efficient and safe.

The present invention relates to hydrogel hyaluronic acid (HA) microspheres or pharmaceutically acceptable salts thereof prepared via suspension polymerization. The hydrogel HA microspheres prepared according to the method of the invention can be used as carriers for various substances, such as carriers for various drug parts. The invention also provides a drug conjugate using the hydrogel HA microsphere as a carrier or a pharmaceutically acceptable salt thereof, a method for preparing the drug conjugate, a drug composition containing the drug conjugate, and applications of the drug conjugate and the pharmaceutically acceptable salt.