199 results about "Hydrogel microspheres" patented technology

The present disclosed subject matter relates to methods and compositions for restoring a diseased or damaged tooth such that infection is inhibited or eliminated and pulp regeneration is facilitated. The disclosed subject matter also includes a composition comprising a physiologically acceptable matrix seeded with pulp cells. The matrix can be capable of being injected into the pulp chamber of a tooth. In some embodiments, the matrix of a composition includes a hydrogel (e.g., collagen, chitosan, alginate, MATRIGEL™, gelatin, JELL-O®, fibrin), a mesh (e.g., polylactide-coglycolide (PLGA) mesh, polylactide (PLA) mesh, or polyglycolide (PGA) mesh, a cross-linked fiber mesh, a nanofiber mesh, a mesh fabric, biodegradable polymer mesh), a microsphere (biodegradable polymer microsphere, a hydrogel microsphere), or a combination of any of the foregoing. In yet other embodiments, the matrix includes a nanofiber, an artificial three-dimensional scaffold material, or a synthetic three-dimensional scaffold material.

The present invention discloses a hollow microsphere preparation method, which uses a hydrogel microsphere as a template. The preparation method is that an emulsion or inverse emulsion polymeric method is adopted to synthesize the hydrogel microsphere with electric charge. A surfactant with inverse electric charge and hydrophobic monomer are added into the hydrogel microsphere suspension, and the hydrogel microsphere is coated by a polymer crust layer of the hydrophobic monomer by a seed emulsion polymerization method to form a core-shell microsphere with an inner layer of hydrogel and an outer layer of polymer; a hydrolyzation of a silicon dioxide or titanium dioxide precursor is used to form the core-shell microsphere with the outer layer of inorganic matter and the inner layer of hydrogel. The core-shell microsphere is dried to obtain the hollow microsphere. The method of the present invention does not need removing the template by organic impregnant dissolving, strong acid or strong alkali etching or high temperature calcining; the preparation process is safe and the operation is simple; and the hollow microsphere with different chemical components can be obtained by changing core or shell.

The invention relates to an alginate-polycationic microcapsule product used for embedding bioactive substance, in particular to an alginate-polycationic microcapsule subjected to graft modification of dopamine. According to the alginate-polycationic microcapsule subjected to graft modification of dopamine, dopamine molecules are in covalent bonding to sodium alginate molecules, the obtained molecules are used for preparing a hydrogel microsphere carrier, the embedded hydrogel microsphere carrier further has film formation reaction with polycations, and thus the dopamine-based alginate / polycationic microcapsule is prepared. The microcapsule has good stability, and does not swell or break in the body fluid environment. The product is mainly used for embedding the bioactive substance, the microcapsule film has the excellent biocompatibility while having the good film strength, and the integrity of the microcapsule film when taken as the histocyte transplantation carrier, the cell carrier of the in-vitro bioartificial liver system, the cell carrier in the cell culture process and the like in the application process is guaranteed.

Injectable hydrogel microspheres are prepared by forming an emulsion where hydrogel precursors are in a disperse aqueous phase and polymerizing the hydrogel precursors. In a preferred case, the hydrogel precursors are poly(ethylene glycol) diacrylate and N-isopropylacrylamide and the continuous phase of the emulsion is an aqueous solution of dextran and a dextran solubility reducer. The microspheres will load protein, e.g., cytokines, from aqueous solution.

The invention discloses a nano particle compound and a preparation process thereof. The nano particle compound which is provided by the invention is formed by aquogel microsphere and inorganic nano particles, wherein the inorganic nano particles are jogged in the aquogel microsphere, the aquogel microsphere is isopropyl acrylamide and acroleic acid copolymer aquogel microsphere. The preparation process of nano particle compound comprises following steps that firstly preparing isopropyl acrylamide and acroleic acid copolymer aquogel microsphere, and dispersing aquogel microsphere into water, and dispersing inorganic nano particles into water, secondly mixing two kinds water solution which are obtained in first step, wherein the mass ratio of isopropyl acrylamide and acroleic acid copolymer aquogel microsphere and inorganic nano particle in mixing solution is 1:10-1:20, stirring mixing solution for 5-10 minutes under the conditions of temperature is 25-35 DEG C, and pH is 5-7, thereby obtaining nano particle compound.

The utility model relates to a rapid preparation method of SiO2 aerogel sphere, belonging to a field of inorganic metallic materials, in particular to a method of rapidly preparing SiO2 aerogel sphere. Water glass is used as raw material that performs ion exchange to obtain the silicic acid PH=2-3, after added with alkaline catalyst, the silicic acid is added into the hydrophobic oil, and then is mixed to cut into micro liquid drops that are spherically suspended in the oil under the action of surface tension, the silicic acid is mixed until aerogel sphere is generated, afterwards, the oil and the sphere perform solid-liquid separation, and then are cleaned with water to obtain aerogel sphere, the aerogel sphere is soaked by the ethanol and absolute alcohol in sequence to perform solvent exchange to obtain alcohol gel sphere, after dried, aerogel sphere with micrometer or millimeter grade particles can be otained. The invention has low cost, easy and controllable process, mass production, good product quality and wide application prospect in the heat isolation and catalysis fields.

The invention relates to a preparation method of an aceptic packaging material with a release-controlling function, particularly comprising three steps: firstly, a chemical surface modifying method is adopted to cause the surface of polymer packaging materials to form certain amount of dissociable functional groups such as carboxyl, sulfonic group, amidine, etc; meanwhile, antibacterial agent nisin is coated inside chitosan hydrogel microspheres which employ carboxyl polyethylene glycol as crosslinking agent, employ water-soluble carbodiimide hydrochloride as coupling agent and have particle size smaller than 100nm; in mixed solvent with 100 / 0-50 / 50 of water / ethanol, the drug-coating chitosan microspheres through ultrasonic stirring processing is 'fixed' on the surface of modified polymer thin films through electrostatic absorption between cathodic anodic ion. Since the surface modifying methods and chitosan hydrogel formulas are different, the releasable nisin amount can reach 0.5-40 Mu g / cm<2>.

The invention belongs to the field of high polymer materials, and relates to a method for preparing a nano fluorescence thermometer based on thermosensitive controllable nano microspheres with fluorescence property. The method comprises the following steps of: synthesizing core / shell-structured polymer nano microspheres with thermosensitive characteristic; and compounding fluorescent molecules with aggregation-induced emission (AIE) property into the shell layers of the microspheres to realize sudden fluorescence enhancement of the fluorescent molecules so as to obtain the thermosensitive controllable nano hydrogel microspheres with the fluorescence property, wherein the fluorescence intensity of the microspheres is linearly reversibly reduced or enhanced with the rise and reduction of the ambient temperature, and the microspheres have high stability in a range of 2 to 95DEG C. The thermosensitive hydrogel material has high application value and good prospect in the aspects such as nano fluorescence thermometers, nano-biomaterials, multi-response sensors and the like.

Dimensionally stable, shaped articles comprised of dried, aggregated, water-swellable hydrogel microspheres are described. The microspheres are aggregated together without the use of a binding agent. When exposed to an aqueous medium in a container, the dimensionally stable, shaped article swells slowly and disaggregates, forming at least partially swollen hydrogel microspheres, which take the shape of the container. The dimensionally stable, shaped articles disclosed herein have many potential applications, including medical applications such as a medical implant.

The invention discloses a preparation method of magnetic microspheres with a visible glucose detection function. The preparation method comprises the following steps: by utilizing a photonic crystal microsphere as a template, preparing magnetic photonic crystal hydrogel microspheres with reverse opal structures through a template copy method of hydrogel. A detection method comprises the steps that: a glucose molecule identification unit is combined with the magnetic photonic crystal hydrogel microspheres, wherein the existence or number of the glucose molecules is expressed by the displacement or color of the reflection peak of the photonic crystal microsphere; a magnet is used for collecting the magnetic microspheres according to the property that the magnetic microspheres have magnetism to improve the naked eye observation accuracy of the color of the magnetic microspheres. The visible glucose detection method based on magnetic microspheres with visible glucose detection function has the advantages of high sensitivity, strong specificity, simple operation, low cost, accurate observation, and the like.

The invention discloses a preparing method of a magnetic hydrogel microsphere, and belongs to the field of sewage treatment. The method includes firstly preparing graphene oxide (GO@Fe<3>O4) loaded with Fe<3>O4 magnetic nanometer particles through a coprecipitation process, mixing the GO@Fe<3>O4 with a water soluble polymer (such as sodium carboxymethylcellulose (CMC)), and preparing the magnetic composite hydrogel microsphere (such as CMC / GO@ Fe<3>O4) through a spray drying process. The prepared composite hydrogel microsphere has good swelling properties and a large specific surface area. The adsorption efficiency for pollutants is increased, and pollutant adsorption varieties and adsorption amounts of hydrogel are increased. The composite hydrogel microsphere is easy to separate, and can be adopted as adsorbents for organic dye and heavy metal ions in industrial waste water.

The invention provides an emulsion-based method for the preparation of controlled release microspheres for the delivery of active compounds. The method comprises the preparation of an emulsion comprising an aqueous dispersed phase which comprises a polymer capable of forming a hydrogel, a bioactive protein, and water, and which is substantially free from insoluble aggregates of the bioactive protein. Subsequently, the polymer physically or chemically crosslinked to form a hydrogel. The invention further provides active protein-loaded hydrogel microspheres which are prepared by the process, and which are substantially free from insoluble aggregates of the active protein. The microspheres exhibit controlled release, with release profiles which are considerably improved over those of previously known hydrogel microspheres. The microspheres may be used to deliver therapeutic or diagnostic proteins by injection.

The invention discloses prussian blue nano-scale hollow olivary microballoons, and belongs to the technical field of prussian blue materials. A preparation method of the prussian blue nano-scale hollow olivary microballoons comprises the following steps that 1, acrylamide as a monomer, N,N'-methylenebisacrylamide as a cross-linking agent, ammonium persulfate as an initiator and ethanol as a solvent are prepared into crosslinked polyacrylamide hydrogel microballoons by a dispersion polymerization technology; 2, hydrogel microballoon / ethanol suspending liquid is added with a Fe<3+> salt aqueoussolution; 3 the mixed solution obtained by the step 2 is stirred violently for one night so that the crosslinked polyacrylamide hydrogel microballoons swelled by Fe<3+> deform; 4, a potassium ferrocyanide aqueous solution is added dropwisely into the mixed solution treated by the step 3 to form prussian blue shells; and 5, the solvent is removed so that the prussian blue nano-scale hollow olivarymicroballoons are obtained. The preparation method of the prussian blue nano-scale hollow olivary microballoons has the advantages of simple operation, low cost, good adaptability for large-scale production, and controllability of microballoon sizes. The prussian blue nano-scale hollow olivary microballoons have nano-scale short axis sizes and micron-scale long axis sizes, and can be utilized widely for drug slow release, sensors, electrode materials and the like.

A hydrogel microdome that can swell in response to a stimuli or target molecule is formed by polymerizing a mixture comprising a monomer capable of forming a hydrogel with a biopolymer. An array of hydrogel microdomes can be formed on a substrate by microspotting the mixture and polymerizing. The array can be used for high-throughput screening of analytes as well as for use as an actuator and biosensor using the swelling property of the hydrogel.

Disclosed are processes for making hydrogel microspheres. More particularly, the present disclosure is directed to processes for making hydrogel microspheres by electrospraying and a controlled, timed gelation.

The invention provides a preparation method of hydrogel porous microspheres. The preparation method comprises the following steps: a, a hydrogel material, a buffer solution and a photoinitiator are mixed to obtain a hydrogel solution, and an oily material is mixed with a surfactant to obtain an oily solution; b, a first container is partially nested in a second container, the inner diameter of theinlet end of the first container is larger than the inner diameter of the outlet end of the first container, and the inner diameter of the outlet end of the first container is smaller than the innerdiameter of the second container; the hydrogel solution is injected into the first container, the oily material is injected into the intersection of the first container and the second container, and the oily solution and the hydrogel solution form water-in-oil droplets under the action of a flowing shear force; c, ultraviolet irradiation is performed on the water-in-oil droplets, and crosslinkingis performed to obtain hydrogel microspheres; and d, freezing and freeze-drying are performed on the hydrogel microspheres to obtain the hydrogel porous microspheres. According to the invention, uniform and stable hydrogel porous microspheres are obtained by utilizing a micro-fluidic technology.

The invention belongs to the field of medical biological technique and discloses a method for digitally detecting micro-mutation by using a micro-emulsion clone amplified bound water gel microsphere chip. The method detects the micro-mutation by combining the microsphere mediated micro-emulsion clone amplifying technique with the water gel chip fixing microsphere fluorescence detection and digitization analysis techniques. The method promotes the positive relevance ratio of micro-mutation, has low cost, high sensitivity and fine specificity and is applied to quantitatively measuring micro-mutation of various low abundance tumor relative genes.

A molecularly imprinted hydrogel dispersed system having self-expression characteristic, belonging to the technical field of medicine detection, is constructed at molecule, nanometer and micrometer level based on the comprehensive application of molecular imprinting, photonic crystals, sol-gel and other nanometer preparation technologies. An area to be detected is coated with photonic crystal hydrogel ink at normal temperature, and the red shift of the photon band gap of a photonic crystal hydrogel microsphere occurs and the color changes as imprinting molecules are dissolved in a solvent, so substances which can be detected with the hydrogel microsphere exist in the area to be detected. The microsphere can be applied to the detect of prohibited pesticides, toxins and heavy metal quantum dot nano-particles.

The invention belongs to the technical field of hydrogel polymer microsphere preparation and relates to a method for preparing hydrogel microspheres based on a microfluidic chip technology and a device for implementing the preparation method. According to the method, an aqueous mixture of a water-soluble monomer, a crosslinking agent, an initiator and a pore-forming agent is used as a dispersion phase, an oil mixture of a water-insoluble liquid and lipophilic hydrophilic amphiphilic molecules is used as a continuous phase, the aqueous mixture and the oil mixture form micro-scale water-in-oil droplets in a microfluidic chip, the droplets are collected, cured and demulsified and monodisperse micro-scale hydrogel microspheres are recovered. The monodisperse micro-scale hydrogel microspheres have the advantages of controllable and uniform grain diameters and high recoverability and can be widely used in technical fields of gene immobilization and screening, protein and cell coating, and a drug slow release system.

The invention provides polymer hydrogel microspheres and a preparation method thereof. The preparation method comprises the following steps of: carrying out copolymerization reaction on a thermosensitive polymerized monomer, a hard hydrophobic polymerized monomer and a polymerized monomer with negative electricity functionality by adopting a one-step emulsion polymerization method; and reacting the obtained polymer with thermosensitive response with a rare earth complex to obtain the polymer hydrogel microspheres with fluorescence properties. According to the method, a polymer hydrogel with thermosensitivity is obtained through one-step method emulsion polymerization, and then the polymer hydrogel is used for reacting with the rare earth complex to obtain the needed product, so that the method is simple in process, the consumed time is shortened, and the production cost is relatively low. Moreover, the polymer hydrogel microspheres provided by the invention have a property of temperature response and good fluorescence optical properties, are intelligent materials with multi-responsiveness, can be uniformly dispersed in water, can be controllably and automatically assembled on a hydrophilic base material easily, and have important values in the fields of optical materials, biological medicine and the like.

The invention belongs to the fields of supramolecular polymer polymer chemistry and drop formation control, and particularly relates to supramolecular hydrogel microsphere modified with cyclodextrin water-solubility polymer and adamantine water-solubility polymer and formed by assembling subjective and objective bodies in a liquid drop, and a preparation method and application thereof. The first purpose of the invention is to provide novel supramolecular hydrogel microsphere formed by assembling subjective and objective bodies and a preparation method thereof method, the second purpose of the invention is to adopt liquid drop as a template to realize supramolecular assembly and achieve uniform particle size and controllable dimension of the supramolecular hydrogel microsphere, and the third purpose of the invention is to provide application of the type of supramolecular hydrogel microsphere in adriacin doxorubicin and taxol water-solubility antitumor drug sustained release, so as to achieve the purpose that a drug can keep a high concentration level for a long time.

The invention discloses a graphene aerogel microsphere and a preparation method thereof. The graphene aerogel microsphere contains a porous structure formed by aggregation of graphene. Aperture of pores contained in the porous structure is 1-500 nm, specific surface area of the graphene aerogel microsphere is 100-1000 m<2> / g, bulk density is less than 300 mg / cm<3>, and specific capacitance is 30-500 F / g. The preparation method comprises the following steps: providing a graphene oxide aqueous dispersion; mixing the graphene oxide aqueous dispersion and a reducing agent to form mixed liquor; spraying the mixed liquor to form monodispersed micro-droplets, carrying the micro-droplets by powder formed by aggregation of hydrophobic particles to obtain liquid marbles of the graphene oxide aqueous dispersion; turning the liquid marbles into gel to obtain graphene aerogel microsphere; and removing hydrophobic particles, and displacing through a solvent and drying to obtain the graphene aerogel microsphere. The method of the invention is simple. The prepared graphene aerogel microsphere has uniform and controllable size, has excellent performance, can realize continuous production, and has a huge application prospect.

The invention relates to hydrogel microspheres based on glucan and a preparation method thereof, which belong to the technical field of biomedical polymer materials. The method comprises the following steps: dissolving glucan in water at room temperature, adding sodium periodate to obtain a mixed liquor, stirring and dialyzing the mixed liquor, performing freeze drying on the mixed liquor to obtain a partial aldehyde glucan solid; dissolving the partial aldehyde glucan solid in deionized water, adding a cyclohexane solution with a dissolved emulsifier, adding an amine crosslinker and stirring the solution, finally collecting precipitate through centrifugation, cleaning the precipitate to obtain the chemical cross-linking type glucan hydrogel microspheres; adding sodium borohydride in the chemical cross-linking type glucan hydrogel microspheres dispersed in deionized water, stirring the material for 24 hours under room temperature, finally collecting the precipitate through centrifugation, and cleaning the precipitate by the deionized water to obtain the reduced glucan hydrogel microspheres. According to the invention, a water-in-oil anti-phase microemulsion system is taken as a reaction medium, and the glucan hydrogel microspheres with a controllable particle size and good stability can be prepared.

The invention discloses a sodium alginate / carboxylated cellulose nanocrystal hydrogel microsphere for adsorbing lead ions. The sodium alginate / carboxylated cellulose nanocrystal hydrogel microsphere comprises, by weight, 5-8 parts of sodium alginate, 6-8 parts of carboxylated cellulose nanocrystals, 0.5-0.52 parts of divinyl sulfone, 150-180 parts of distilled water and 30-40 parts of a 0.1-0.2 mol / L calcium chloride solution. The sodium alginate / carboxylated cellulose nanocrystal hydrogel microsphere has the following advantages: the sodium alginate and microcrystalline cellulose which are used as raw materials in the method are natural materials, so the microsphere is nontoxic and harmless, is friendly to the environment, and realizes appreciation and full utilization of materials as a water purifier; and the prepared hydrogel microsphere has a good homogeneity, a uniform particle size, no toxic or side effects, a large specific surface area and a large porosity, has high adsorptioncapability to lead ions and other heavy metal ions, can be used as a safe and efficient lead ion adsorbent, and has a broad industrial application prospect.

The invention discloses a hydrogel microsphere fluorescence sensor as well as a preparation method and application thereof. By use of the advantages of a hydrogel material, the hydrogel material is applied to wrapping a fluorescence probe; the hydrogel is fully mixed with the fluorescence probe, and the mixture is directly processed into a fluorescence sensor system which has a microsphere structure and is applicable to fluorometric analysis and detection by virtue of an ultraviolet polymerization reaction; successfully cured hydrogel micropheres have favorable flexible structures and can not release hydrogel monomers without influencing detection; due to a net-shaped structure, the wrapped fluorescence probe is quite stably dispersed in the hydrogel with out leaking through the micropheres; detection substances can freely enter into or move out of the whole microsphere fluorescence sensor system; in combination with a fluorescence microscope, the hydrogel microsphere fluorescence sensor is capable of quite conveniently analyzing and detecting ions, molecules and other relevant substances; the hydrogel microsphere fluorescence sensor is a microsphere which is formed by wrapping the fluorescence probe with hydrogel and has a diameter of 300-500 mu m.

A hydrogel microdome that can swell in response to a stimuli or target molecule is formed by polymerizing a mixture comprising a monomer capable of forming a hydrogel with a biopolymer. An array of hydrogel microdomes can be formed on a substrate by microspotting the mixture and polymerizing. The array can be used for high-throughput screening of analytes as well as for use as an actuator and biosensor using the swelling property of the hydrogel.

The invention discloses a thermo-sensitive coding microsphere carrier for drug controlled release and a preparation method of the microsphere carrier. The carrier is a hydrogel microsphere with an inverse opal structure. The main component of the microsphere is poly(N-isopropylacrylamide), which has a three-dimensional ordered nanometer porous structure for carrying multiple protein or polypeptide medicines. Contraction and expansion of nanometer holes can be controlled through temperature regulation, so that drug controlled release is achieved. The inverse opal hydrogel microsphere is prepared by taking a colloidal crystal microsphere as a template. The carrier is simple in preparation method, low in cost and convenient for large-scale production. The prepared coding carrier is non-toxic and is good in biocompatibility and controllability.

The invention discloses hydrogel microspheres embedded with coagulation related enzyme and a preparation process of the hydrogel microspheres. The hydrogel microspheres and the preparation process have the advantages that the particle size of the prepared hydrogel microspheres is 100-1000 micrometers and is uniform (CV<20%), the coagulation related enzyme is evenly distributed in the hydrogel microspheres, the mass ratio of the coagulation related enzyme to sodium alginate is 1:3-4:1, and the drug loading capacity of the coagulation related enzyme after dewatering is 25-80%.

The invention belongs to the technical field of chemical and biochemical medicines, particularly relates to a DNA fixed nano hydrogel microsphere, a preparation method of the DNA fixed nano hydrogel microsphere and a nucleic acid aptamer compound and an application of the DNA fixed nano hydrogel microsphere as a tumor targeting preparation. The preparation method is simple, only a segment of single-stranded DNA is synthesized at one end of the nucleic acid aptamer as a connecting arm when the nucleic acid aptamer is synthesized, and the sequence of the DNA connecting arm and the DNA sequence on the surface of the nano material of the nano hydrogel microsphere can be complemented and paired. One or a plurality of nucleic acid can be matched and connected on the surface of the nano materialat one time by co-incubation in aqueous solution so as to obtain the drug delivery carrier with the specific targeting function. According to the method, one or more nucleic acid aptamers can be connected on the surface of the drug delivery carrier at one time in a base complementary pairing mode; the base complementary pairing can be carried out in aqueous solution; reaction conditions are mild and easy to control; and the preparation process is very simple.