257 results about "Hydrogel microspheres" patented technology

The present invention relates to the field of biomedical materials. Particularly provided is a DNA-silk fibroin composite hydrogel microsphere. The DNA-silk fibroin composite hydrogel microsphere is prepared from DNA and silk fibroin on the basis of the micro-fluidic technology by means of combination of self-assembly physical crosslinking and photo-crosslinking. The introduction of a silk fibroin network into the composite hydrogel microsphere can provide mechanical performance for a DNA hydrogel network and reduce the production cost. RGD can remarkably improve the cell loading capability of the microsphere. The hydrogel microsphere can simulate a microenvironment of cartilage tissue by means of three-dimensional loading of high-density cells, so as to enhance substance exchange and signaling between cells, and more effectively promote the proliferation and adhesion of bone marrow mesenchymal stem cells and regulating the chondrogenic differentiation, thus promoting cartilage regeneration and repair, and providing an available material for preparing medicaments or medical instruments for cartilage defect repair.

The invention belongs to the technical field of biomedical materials, and particularly relates to a multi-layer multifunctional hydrogel microsphere for diabetic wounds as well as a preparation method and application of the multi-layer multifunctional hydrogel microsphere. The preparation method comprises the following steps: firstly, synthesizing a chitosan derivative with proper amino and aldehyde group contents, oxidized macromolecules and modified chitin; then preparing photocuring hydrogel microspheres by utilizing a microfluidic technology, and sequentially immersing the photocuring hydrogel microspheres into an oxidized polymer solution, a modified chitin solution, a tannic acid solution and a metal salt solution, so as to construct the hydrogel microspheres with a multifunctional layer structure. The invention further provides specific application intervals of parameters such as solvents used by various solutions, concentration ranges and soaking time. The preparation method provided by the invention has expansibility and universality, various types of microspheres can be constructed for sequential release of bioactive substances, and the requirements of tissue repair are met.

The invention belongs to the technical field of drug delivery, and particularly relates to a multi-response colitis disease targeting hydrogel microsphere as well as a preparation method and application thereof. The hydrogel microsphere comprises a calcium ion cross-linked sodium alginate microsphere and HA-Cys / Cur NPs which is encapsulated in the calcium ion cross-linked sodium alginate microsphere, the preparation method comprises the following steps: self-assembling cysteine ethyl ester modified hyaluronic acid and curcumin to form nano-particles, encapsulating HA-Cys / Cur NPs into sodium alginate microspheres by an emulsification method, and carrying out calcium ion crosslinking, so as to obtain the hydrogel. The hydrogel microspheres have pH and GSH response characteristics and hyaluronic acid-mediated active targeting capability, are stable in a gastric acid environment, are accurately released at a colon part and target macrophages at an inflammation part, and NPs disconnects disulfide bonds and releases curcumin in an intracellular high GSH environment, so that synergistic treatment of anti-inflammation, anti-oxidation and intestinal mucosa repair is realized, and the curative effect is good. The medicine has a good application prospect in treatment of ulcerative colitis.

The invention relates to a two-phase hydrogel system based on space-time gradient regulation and control as well as a preparation method and application of the two-phase hydrogel system. The biphase hydrogel system can also be expressed as a GelMA composite hydrogel scaffold of a porous structure, the whole biphase hydrogel system is of a core-shell structure, the inner layer core mineralizes SilMA hydrogel microspheres, the porous GelMA hydrogel scaffold is used as an outer layer, and the angiogenesis promoting factor dimethyl oxalaminoacetic acid, the human umbilical vein endothelial cells and the bone marrow mesenchymal stem cells are loaded on the outer layer. Wherein the inner-layer core mineralized SilMA hydrogel microspheres realize continuous release of osteogenesis signals by slowly releasing nHAp, and the outer-layer porous GelMA hydrogel stent releases DMOG by rapid degradation to realize early angiogenesis, so that space-time coupling construction of blood vessels and bone tissues is realized. Through differential degradation characteristics of inner and outer layers of hydrogel, precise time sequence cooperative regulation and control of angiogenesis and bone tissue remodeling can be realized, and the bionic property and function integration capability of a vascularized bone organ structure can be improved, so that the clinical transformation potential of the vascularized bone organ structure is improved.

The invention relates to an organ-like microsphere embedding slicing method. The method specifically comprises the following steps: 1) preparing hydrogel microspheres and dyeing the hydrogel microspheres with fuchsin; (2) dehydrating the dyed hydrogel microspheres and the organoid together, then putting the dehydrated hydrogel microspheres and the organoid into an embedding mold, adding an embedding agent for embedding, and obtaining an embedded sample after the embedding agent is solidified; and 3) slicing the embedded sample, and receiving the sliced sample by using a glass slide. According to the method, the hydrogel microspheres are firstly dyed, and the dyed hydrogel microspheres and the organoid are embedded together, so that the peripheral area of the organoid can be obviously red, and the magenta dyeing area on the section sample can be clearly observed even after the section sample is sliced. The hydrogel microspheres dyed by fuchsin can be used as positioning marks of organoid-containing microspheres, can provide obvious prompts in the early transparent dehydration process and the later embedding section process, and effectively help an operator to judge the section repairing degree and the section collecting degree.

The invention belongs to the technical field related to tumors, and discloses a preparation method of self-developing embolism microspheres. Comprising the following steps: producing calcium alginate microspheres through cross-linking, obtaining polyelectrolyte hydrogel microspheres through electrostatic complexation, and obtaining the self-developing embolism microspheres through amino cross-linking. According to the invention, sodium alginate and polyamino acid are used as raw materials for preparation, so that the preservative is safe and non-toxic and has biodegradability, and the degradation product has no toxic or side effect on human body. The microspheres are prepared by adopting an electrostatic spraying method, organic solvents are not used, and the microspheres are green and environment-friendly and have no toxic residues.

The invention relates to a heparinized gelatin / sodium alginate pancreas islet packaging material with an anti-fibrosis function as well as a preparation method and application of the heparinized gelatin / sodium alginate pancreas islet packaging material. The preparation method comprises the following steps: adding gelatin into normal saline, soaking, performing autoclaved sterilization to obtain a gelatin solution, then adding epsilon-polylysine hydrochloride into the gelatin solution, and finally adding glutamine transaminase for reaction to obtain an epsilon-polylysine grafted gelatin solution; adding heparin into the solution, and reacting to obtain a heparinized gelatin solution; adding sodium alginate and glutamine transaminase, reacting, diluting, preparing hydrogel microspheres by adopting an electrostatic spraying method, and cross-linking to obtain the hydrogel microspheres. According to the invention, heparin molecules are incorporated into a pancreas islet packaging system, so that fibrosis at the periphery of the microspheres can be relieved, the pancreas islet cells are packaged by using the pancreas islet packaging material to prepare the biological artificial pancreas, the pancreas islet cell proliferation and insulin secretion can be promoted, and the pancreas islet packaging material has a good diabetes treatment effect.

This invention belongs to the field of biopharmaceutical technology and specifically discloses size-controlled bacteriophage hydrogel microspheres and their preparation method. The hydrogel microspheres are composed of bacteriophage, a polymer carrier, and a curing agent and are prepared using electrostatic spray technology. Compared with existing hydrogel microsphere preparation methods, these hydrogel microspheres can significantly prolong the sustained release time of bacteriophage in the intestinal environment and improve their stability in the gastric environment. Furthermore, by adjusting the preparation parameters, the size of the microspheres can be flexibly controlled to meet different application scenarios and needs.

The invention discloses a hydrogel microsphere system for sequential delivery of drugs as well as a preparation method and application thereof, and particularly relates to the technical field of biomedical materials. The microsphere system comprises inflammation responsive hydrogel microspheres obtained by grafting molecules containing inflammation responsive groups with hyaluronic acid as a skeleton, common microspheres obtained by grafting non-responsive molecules with hyaluronic acid as a skeleton, an anti-inflammatory drug and a healing promoting drug. Wherein the molecular weight of the hyaluronic acid is 74-800kDa, the inflammation responsive hydrogel microspheres are loaded with anti-inflammatory drugs, and the common microspheres are loaded with healing promoting drugs. According to the hydrogel microsphere system, the drug release rate and degradation time of the microspheres are adjusted by limiting the molecular weight of hyaluronic acid, and a molecular basis is provided for subsequent differentiated drug release.

The invention discloses a functional hydrogel microsphere and a preparation method and application thereof, and relates to the technical field of medicines.The preparation method comprises the steps that a hyaluronic acid methacrylic acid precursor solution and an IL-1beta shRNA adenovirus stock solution are mixed to obtain an Ad-atHAMA precursor solution; preparing the Ad (at) HAMA precursor solution through an oil-in-water micro-fluidic technology to obtain an Ad (at) HAMA microsphere; and sequentially adding the Ad (at) HAMA microspheres, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide into a 2-morpholinoethanesulfonic acid buffer solution, carrying out activating treatment, adding E selectin, and incubating to obtain the functionalized hydrogel microspheres. The functionalized hydrogel microspheres can inhibit a related mechanism of acute spinal cord injury pathological progress and promote neural function repair by targeting adsorption of neutrophil to regulate Nets-triggered innate immune inflammation cascade.

The invention belongs to the technical field of scale inhibitors, and particularly relates to a slow-release scale inhibitor and a preparation method thereof.The preparation method comprises the steps that 3, 4, 5-allyl trihydroxybenzoate, itaconic acid, sodium hydrogen sulfite, methacryloyloxyethyl-n-hexadecyl-dimethyl ammonium bromide and deionized water are mixed, after water bath heating, an ammonium persulfate aqueous solution is slowly added, and the scale inhibitor is obtained after reaction; the preparation method comprises the following steps: dissolving agar in deionized water, adding polyethylene glycol modified sodium alginate and a scale inhibitor into the deionized water, stirring, dropwise adding into edible oil to obtain gel microspheres, adding the gel microspheres into a calcium chloride aqueous solution, slowly stirring, cross-linking, cleaning and drying to obtain the slow-release scale inhibitor. The hydrogel microspheres swell to slowly release the scale inhibitor, meanwhile, the released scale inhibitor is hydrolyzed in water, ester groups are slowly broken, polycarboxylic acid, 3, 4, 5-trihydroxybenzoic acid and hydroxyethyl-n-hexadecyl-dimethyl ammonium bromide are slowly released, and good scale inhibition and corrosion inhibition effects are achieved.

The invention discloses a hydrogel microsphere sorting method, an artificial organ preparation method, a system and a medium. The method comprises the following steps: acquiring a hydrogel microsphere image in the micro-fluidic chip; analyzing the hydrogel microsphere image through a neural network to obtain an analysis result; based on the analysis result, hydrogel microspheres wrapping the target cells are sorted out; the neural network comprises a feature extraction layer and a multi-scale joint output head, and the hydrogel microsphere image is processed by the multi-scale joint output head after being convolved by the feature extraction layer to obtain an analysis result. According to the method, the hydrogel microspheres wrapped with the target cells are accurately identified and sorted by using a droplet microfluidic technology, a standardized artificial organ can be prepared based on the sorted hydrogel microspheres, and the functional maturity and reliability of the artificial organ are remarkably improved.

The invention discloses an injectable multifunctional intelligent response type strontium-containing hydrogel microsphere as well as a preparation method and application thereof, the strontium-containing hydrogel microsphere comprises a hydrogel microsphere carrier and a loaded medicine, and the hydrogel microsphere is obtained from lycium barbarum polysaccharide and a temperature-sensitive polymer through a microfluidic technology; the loaded medicine is strontium ions and a near-infrared light conversion material, the strontium ions are combined with the lycium barbarum polysaccharide in the microspheres through ion coordination, and the near-infrared light conversion material is combined with the microspheres through physical adsorption. The lycium barbarum polysaccharide and the temperature-sensitive polymer are combined, the bone regeneration promoting capacity is improved through the synergistic effect of the lycium barbarum polysaccharide and the temperature-sensitive polymer, the hydrogel microspheres have intelligent adjustability further through the near-infrared response performance, the strategic purpose of individualized drug delivery in time and space is achieved, and the application prospect is wide. The limitation that a hydrogel carrier cannot be administrated according to needs is avoided, and the hydrogel carrier is small in particle size, injectable and convenient in administration.

The invention relates to core-shell hydrogel microsphere preparation equipment which comprises a microsphere preparation device and a microsphere collection device, the microsphere preparation device is provided with a nuclear phase inlet, a shell phase inlet, a plurality of nuclear phase distribution runners communicated with the nuclear phase inlet, and a plurality of shell phase distribution runners communicated with the shell phase inlet; the nuclear phase distribution flow channels and the shell phase distribution flow channels are converged into coaxial flow channels at the tail ends in a one-to-one correspondence manner; the microsphere collecting device comprises a microsphere collecting pool, the microsphere collecting pool is arranged below the coaxial flow channel, and the microsphere collecting pool is used for containing oil phase fluid; the nuclear phase fluid entering from the nuclear phase inlet and the shell phase fluid entering from the shell phase inlet form layered fluid at the coaxial flow channel, and the layered fluid is dropped into the microsphere collecting pool. According to the core-shell hydrogel microsphere preparation equipment provided by the invention, high-flux preparation of core-shell hydrogel microspheres can be realized.

The invention belongs to the technical field of anticorrosive paint, and discloses intelligent corrosion inhibition hydrogel with double functions of pH sensitivity and corrosion self-early warning, preparation of the intelligent corrosion inhibition hydrogel and application of the intelligent corrosion inhibition hydrogel in modified epoxy resin anticorrosive paint. The preparation method comprises the following steps: preparing a water phase from quaternary ammonium salt chitosan, methacrylic anhydride, a photoinitiator and a corrosion inhibitor, taking a Span emulsifier and paraffin as an oil phase, dropwise adding the water phase into the oil phase, rapidly stirring to form a water-in-oil emulsion system, and rapidly preparing the pH-sensitive hydrogel microspheres coated with the corrosion inhibitor through photo-crosslinking. And finally, forming the corrosion self-early-warning shell by using the tannic acid modified hydrogel microspheres by adopting an electrostatic adsorption layer-by-layer assembly method. The intelligent corrosion inhibitor hydrogel and the modified anticorrosive paint prepared by the invention have double functions of pH sensitivity and corrosion self-early warning. And meanwhile, the material is green and environment-friendly, the preparation process is simple, the cost is low, the corrosion resistance is effectively improved, the corrosion resistance is enriched, and long-acting corrosion prevention is realized.

The invention discloses a periodontal local drug delivery system with antioxidant, anti-inflammatory and antibacterial synergistic effects and a preparation method of the periodontal local drug delivery system, and belongs to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: firstly, preparing an organic phase from DSPC, cholesterol, SA and resveratrol, and preparing a cationic liposome by taking PBS as a water phase; then preparing a water phase from AlgMA and LAP, taking a surfactant solution as an oil phase, and preparing polydopamine hydrogel microspheres through PDA surface modification; and finally, carrying out physical adsorption loading. Through the synergistic effect of the resveratrol, the cationic liposome and the polydopamine, a periodontitis pathological chain is comprehensively intervened, synergistic treatment is achieved, excellent antibacterial performance, anti-inflammatory effect and antioxidant effect are achieved, the retention rate of the medicine is increased, and the problems that traditional treatment medicine cannot achieve antibacterial, inflammation regulation and oxidative stress synergistic treatment at the same time, and the treatment effect is poor are solved. The residence time of the medicine in the periodontal pocket is short, and frequent administration is needed.

The invention relates to the technical field of medical instruments, and discloses mineralized collagen hydrogel microspheres, a preparation method thereof and a bone repair material, the mineralized collagen hydrogel microspheres are prepared from mineralized collagen and sodium alginate composite liquid through electrospray, sodium alginate serves as a carrier of mineralized collagen, the degradation rate of the mineralized collagen is remarkably reduced, and the bone repair effect is improved. Meanwhile, the gel microspheres also have a higher specific surface area, so that the attached cells can quickly form tissues; according to the bone repair material, normal saline and mineralized collagen hydrogel microspheres are used, mineralized collagen in the mineralized collagen hydrogel microspheres can remarkably improve the corrosion resistance of sodium alginate in the normal saline, and the slow release effect of hydrogel in the mineralized collagen hydrogel microspheres can be remarkably improved.

The invention relates to the technical field of hydrogel microspheres, and particularly discloses hydrogel microspheres as well as a preparation method and application thereof. Sodium alginate and acrylamide are used for constructing a stable hydrogel skeleton structure with a certain mechanical property, and in a specific oil phase-hydrogel solution system, the release time and the release rate of Fe ions from an EDTA-Fe complex are accurately controlled by adjusting the pH value, so that the stability and the stability of the hydrogel are improved. The iron ions are released at proper time and rate and participate in the crosslinking reaction of the hydrogel, so that the hydrogel microspheres which are uniform in crosslinking, stable in performance and capable of accurately regulating and controlling the metal ions are prepared; besides, the lithium chloride can enhance the structural stability of a hydrogel network and adjust the plasticity of the hydrogel, so that the hydrogel is more flexible, and a uniform microsphere structure is favorably formed in the stirring and dispersing processes. The hydrogel microspheres prepared by the method have wide application prospects in the fields of drug controlled release, biosensing, environmental restoration and the like.

The invention provides a preparation method of gas-jet mineralized collagen-hydrogel microspheres, and relates to the technical field of biological materials, and the preparation method comprises the following steps: dissolving collagen in a phosphate solution, dropwise adding the phosphate solution and a CaCl2 solution into a continuously stirred alkaline solution together, reacting for 12-48 hours, centrifugally collecting a precipitate, washing with water, centrifuging, and freeze-drying to obtain mineralized collagen; the method comprises the following steps: dissolving mineralized collagen and sodium alginate in deionized water, spraying the solution into a CaCl2 solution through a gas spraying spinning head by adopting a gas spraying technology, collecting microspheres, and repeatedly cleaning by adopting deionized water to obtain the gas spraying mineralized collagen-hydrogel microspheres. Mineralized collagen is encapsulated and loaded through microspheres, so that interferences of tissue fluid are isolated, and non-specific aggregation is reduced; optimizing the ratio of mineralized collagen, and directionally inducing stem cells to differentiate; the diameter of the microsphere is accurately controlled by regulating and controlling gas injection parameters to adapt to different wounds; active ingredients are controlled according to needs through a microsphere cross-linked network, and the multi-stage requirements of subcutaneous repair are met.

The invention discloses a bacteriophage gel microsphere as well as a preparation method and application thereof. The preparation method is simple and easy to implement and convenient to operate and popularize. Meanwhile, the prepared bacteriophage gel microspheres are high in loading capacity and can effectively load bacteriophages, the high porosity and the porous structure can provide a large number of binding sites for the bacteriophages, the contact opportunity of the bacteriophages and bacteria is increased, and the antibacterial efficiency is improved. In addition, the hydrogel property of the bacteriophage gel microspheres can protect the bacteriophage from being influenced by adverse environmental factors such as dryness and high temperature, and the stability of the bacteriophage in practical application is improved. In addition, compared with bacteriophage hydrogel microspheres prepared in the prior art, the bacteriophage gel microspheres prepared in the invention have smaller particle size, so that multi-way administration modes such as treatment of pulmonary infection by atomization administration and the like can be carried out.

The application discloses a preparation method of a high-activity multi-level-pore boronic acid imprinting hydrogel microsphere adsorbent and application of the adsorbent to selective adsorption of a flavonoid compound naringin (NRG). The application enhances surface functionalization of COFs by dopamine modification, prepares thiol functionalized covalent organic framework microspheres, synthesizes thiol microspheres by using an emulsion interfacial polymerization method, prepares COFs boron affinity imprinting microsphere adsorbents based on boron affinity, and applies the adsorbents to selective adsorption of NRG in agricultural wastewater. The prepared adsorbent has a significant multi-layer adsorption effect, and the adsorption capacity is significantly improved by using boron affinity. The multi-level-pore structure of the COFs microspheres provides abundant sites for adsorption, and significantly improves the mass transfer rate. In addition, the prepared adsorbent has significant specificity in adsorption and separation of NRG, and provides a promising adsorption material for industrialized separation of NRG.

The present invention provides an injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere, an articular cavity injection formulation, and a preparation method therefor. The method comprises the following steps: S1, preparing a magnesium-loaded multilamellar vesicular liposome suspension containing magnesium ions; S2, preparing an uncrosslinked magnesium-containing hydrogel; and S3, mixing the magnesium-loaded multilamellar vesicular liposome suspension with the magnesium-containing hydrogel to prepare hydrogel droplets, and carrying out crosslinking to form the magnesium-loaded lipid-based super-lubricating hydrogel microspheres. According to the present invention, the magnesium loading capacity of the lipid-based super-lubricating hydrogel microspheres can be greatly improved; on one hand, the biological function provided by the magnesium ions can be greatly prolonged, that is, the repair of cartilage by synovial liquid progenitor cells is continuously promoted; and on the other hand, the intrinsic lubricating effect of the lipid-based super-lubricating hydrogel microspheres can mitigate the further wear of newly formed cartilage; in addition, the magnesium ions can significantly promote the expression of PRG4 in chondrocytes, which not only facilitates the maintenance of chondrocyte homeostasis, but also further enhances articular lubrication.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a pH-responsive curcumin sustained-release microsphere hydrogel, a preparation method and application thereof in spinal cord injury repair materials. The preparation method is as follows: short fibers are prepared by pretreating and grinding discarded eggshell membranes; after sodium alginate is dissolved, the sodium alginate is blended with the eggshell membrane short fibers, curcumin is added, and stirring is uniformly performed to form a mixed solution; the mixed solution is injected into a calcium chloride solution by using a drip crosslinking method to form, and an eggshell membrane and sodium alginate curcumin microsphere hydrogel is obtained. The hydrogel has excellent water content, swelling performance, biocompatibility, blood compatibility and drug sustained-release function, and can be applied to spinal cord injury repair.

The invention provides Cr (VI) ion imprinted biomass carbon aerogel microspheres and a preparation method and application thereof.The preparation method comprises the steps that a bubble-rich mixed solution of gelatin, chitosan, glacial acetic acid, sodium bicarbonate and lauryl sodium sulfate is dripped into alkali liquor, and Cr (VI) ion imprinted biomass hydrogel microspheres are obtained through a surface ion imprinting technology; and carrying out freeze drying and high-temperature carbonization to prepare the Cr (VI) ion imprinted biomass carbon aerogel microspheres. The inner part of the large-hole interconnected thin-wall structure is of a three-dimensional interpenetrating network structure. When the pH value is equal to 2, the Cr (VI) ion imprinting biomass carbon aerogel microsphere has a Cr (VI) ion removal rate of 99% or above, has a wide pH working range, and has a Cr (VI) ion removal rate of 70% or above when the pH value is equal to 1-5. In the presence of interfering ions, the selectivity coefficient K value of the Cr (VI) ion imprinted biomass carbon aerogel microspheres to Cr (VI) ions is far greater than 1, and the Cr (VI) ion imprinted biomass carbon aerogel microspheres have a relatively large relative selectivity coefficient K'value, and still show the highest adsorption capacity to the Cr (VI) ions in the coexistence of various heavy metal ions.

The invention relates to the technical field of microsphere manufacturing, in particular to a device and method for preparing hydrogel microspheres wrapping organoid and application, by changing the shape and spatial distribution of a high-voltage electrostatic field, the size of the hydrogel microspheres is controlled through a voltage single variable, the preparation efficiency of the hydrogel microspheres is improved, and the hydrogel microspheres are prepared. The problems that the hydrogel microspheres prepared by a traditional high-voltage static non-contact device are large in surface roughness and the sodium alginate hydrogel microspheres are prone to fibrosis during in-vivo transplantation are solved.

The present application relates to a kind of core-shell hydrogel microsphere preparation device.The core-shell hydrogel microsphere preparation device includes microfluidic chip and hydrophobic connecting pipe, the microfluidic chip is equipped with core phase flow channel, shell phase flow channel, water same axis flow channel, oil phase flow channel, ball forming cavity and outlet flow channel, the end of the core phase flow channel and the shell phase flow channel meet, the meeting point is connected with the first end of the water same axis flow channel, the water same axis flow channel and the end of the oil phase flow channel meet in the ball forming cavity, the ball forming cavity is communicated with the first end of the outlet flow channel, the inner diameter of the ball forming cavity is 0.2~2 mm, the inner diameter of the outlet flow channel is 0.4~2 mm, the outer diameter of the hydrophobic connecting pipe is matched with the inner diameter of the outlet flow channel, the inner diameter of the hydrophobic connecting pipe is less than or equal to the inner diameter of the ball forming cavity, one end of the hydrophobic connecting pipe is inserted into the outlet flow channel.

This invention relates to the field of microsphere manufacturing technology, and in particular to a device, method and application for preparing hydrogel microspheres for encapsulating organoids. This invention improves the preparation efficiency of hydrogel microspheres by changing the shape and spatial distribution of the high-voltage electrostatic field and controlling the size of hydrogel microspheres by voltage as a single variable. It also addresses the problems of large surface roughness of hydrogel microspheres prepared by traditional high-voltage electrostatic non-contact devices and the easy fibrosis of sodium alginate hydrogel microspheres during in vivo transplantation.

The invention discloses a lipidosome composite hydrogel microsphere for repairing damaged intestinal mucosa and a preparation method thereof, LHMs (at) 4-OI is composed of hyaluronic acid modified targeted drug-loaded lipidosome, sodium alginate, hydrochloric acid treated sucralfate and a CaCl cross-linking agent, and rapid gel forming is carried out under the voltage of 11.0 KV through an electrospray technology. The microspheres have pH responsiveness and gastric juice digestion resistance, CD44 receptor targeting is achieved through hyaluronic acid modification, sodium alginate and Ca form an egg box structure to provide a three-dimensional network, and sucralfate enhances adhesion to isolate harmful substances. The preparation method comprises the steps of preparation of the drug-loaded liposome by a film hydration method, hyaluronic acid modification and electrospray crosslinking, is simple to operate and low in cost, and can be used for large-scale production. The microspheres can effectively load an anti-inflammatory drug 4-OI, inhibit expression of inflammatory factors and promote self-repair of damaged intestinal mucosa, and have wide application prospects in the fields of abdominal infection, inflammatory bowel diseases, colorectal cancer and the like.

The present application discloses a Micro LED fluid assembly method based on microfluidics and dielectrophoresis, comprising the following steps: S1: making the Micro LED chip into Micro LED chip hydrogel microspheres; S2: suspending the Micro LED chip hydrogel microspheres in a liquid; S3: sorting out the Micro LED chip hydrogel microspheres that meet the performance requirements; S4: evenly distributing the Micro LED chip hydrogel microspheres on the target substrate, and then assembling them using dielectrophoresis traps; S5: the dielectrophoresis traps prepared on the target substrate successively capture the Micro LED chip hydrogel microspheres to form a large-scale ordered array; S6: releasing the Micro LED chip wrapped in the Micro LED chip hydrogel microspheres, which is the corresponding Micro LED chip array; the present application realizes the precise positioning and control of the Micro LED hydrogel microspheres, completes the high-precision transfer of the Micro LED chip, improves the transfer efficiency and positioning accuracy of the Micro LED, simplifies the Micro LED transfer process, reduces the production cost, and has broad market application prospects.

The invention relates to a preparation method of stepped cross-linked myofibril-pectin core-shell hydrogel microspheres, which comprises the following steps: preparing a myofibril protein dispersion liquid and a pectin solution by using a phosphate buffer solution containing NaCl as a solvent; preparing an anthocyanin stock solution by taking a phosphate buffer solution as a solvent; mixing the three components under an ice bath condition, and then reacting at 2-5 DEG C for 1-3 hours; 2) dropwise adding the reaction solution obtained in the step 2) into a continuously stirred CaCl cross-linking solution at 2-5 DEG C, standing and curing after dropwise adding, and washing with distilled water to obtain single-layer hydrogel microspheres with uniform particle size and smooth surface; and naturally draining the surface moisture of the single-layer hydrogel microspheres, soaking the single-layer hydrogel microspheres in a freshly prepared 1-4% pectin solution for 20-40 seconds, then taking out the single-layer hydrogel microspheres, transferring the single-layer hydrogel microspheres into another CaCl crosslinking solution, and continuing crosslinking for 10-20 minutes to form a compact shell structure. And after the reaction is completed, washing with distilled water. The core-shell hydrogel microspheres can be used for detecting volatile ammonia, monitoring and indicating pork freshness and the like.