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Hydrogel microspheres based on glucan and preparation method thereof

A technology of hydrogel microspheres and gel microspheres, which is applied in the field of biomedical polymer materials, can solve problems such as unproposed reaction conditions and surfactant systems, and achieve good biological safety effects

Active Publication Date: 2016-02-24
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The "water-in-oil" inverse microemulsion method has the advantages of mild reaction conditions and easy control of the particle size of gel microspheres in the preparation of hydrogel nanoparticles, so it is a common method for preparing micro / nanoparticle materials ( The Generation of Nanoparticles in Miniemulsions. Advanced Materials, 2001.13: 765-768; Miniemulsion Polymerization and the Structure of Polymer and Hybrid Nanoparticles. Angewandte Chemie International Edition, 2009.48: 4488-4507), but there are few reports about this method for preparing dextran-based hydrogels, and the applicable reaction conditions and methods have not yet been proposed. active agent system

Method used

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  • Hydrogel microspheres based on glucan and preparation method thereof
  • Hydrogel microspheres based on glucan and preparation method thereof
  • Hydrogel microspheres based on glucan and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0034] The structural formula of the dextran-based hydrogel microspheres can be expressed as follows:

[0035] .

[0036] The preparation method of the above-mentioned dextran-based hydrogel microspheres, its specific steps include as follows:

[0037] (1) Preparation of aldylated dextran

[0038] Dissolve 5.0g of dextran (dextran T10, molecular weight 10000g / mol) in water at room temperature according to the solid-to-liquid ratio of 10:100g / ml, and then place it in an ice-water bath according to the sugar unit / IO 4 - Add sodium periodate at a molar ratio of 1:1 to obtain a mixed solution, stir the mixed solution at room temperature and under dark conditions for 4 hours, and dialyze the obtained solution in deionized water for 2 For ~3 days, the deionized water was changed every 3~5h during the dialysis process. After the dialysis was completed, freeze-dried to obtain a partially aldylated dextran solid with a degree of aldylation of 60% (that is, there were 60 sugar unit...

Embodiment 2

[0044] The structural formula of the dextran-based hydrogel microspheres can be expressed as follows:

[0045] .

[0046] The preparation method of the above-mentioned dextran-based hydrogel microspheres, its specific steps include as follows:

[0047] (1) Preparation of aldylated dextran

[0048] Dissolve 5.0g of dextran (dextran T40, molecular weight 40000g / mol) in water at room temperature according to the solid-liquid ratio of 10:100g / ml, and then place it in an ice-water bath according to the sugar unit / IO 4 - Add sodium periodate at a molar ratio of 1:1 to obtain a mixed solution, stir the mixed solution for 4 hours at room temperature and in the dark, and dialyze the obtained solution in deionized water for 2 hours with a regenerated cellulose dialysis bag (molecular weight cut-off: 7kD For ~3 days, the deionized water was changed every 3~5h during the dialysis process. After the dialysis was completed, freeze-dried to obtain a partially aldylated dextran solid wit...

Embodiment 3

[0056] The structural formula of the dextran-based hydrogel microspheres can be expressed as follows:

[0057] .

[0058] The preparation method of the above-mentioned dextran-based hydrogel microspheres, its specific steps include as follows:

[0059] (1) Preparation of aldylated dextran

[0060] Dissolve 5.0g of dextran (dextran T70, molecular weight 70000g / mol) in water at room temperature according to the solid-to-liquid ratio of 10:100g / ml, and then place it in an ice-water bath according to the sugar unit / IO 4 - Add sodium periodate at a molar ratio of 1:1 to obtain a mixed solution, stir the mixed solution for 4 hours at room temperature and in the dark, and dissolve the stirred solution in deionized water with a regenerated cellulose dialysis bag (molecular weight cut-off: 7kD) Dialyze for 2 to 3 days, change the deionized water every 3 to 5 hours during the dialysis process, and freeze-dry after the dialysis to obtain a partially formylated dextran solid with a d...

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Abstract

The invention relates to hydrogel microspheres based on glucan and a preparation method thereof, which belong to the technical field of biomedical polymer materials. The method comprises the following steps: dissolving glucan in water at room temperature, adding sodium periodate to obtain a mixed liquor, stirring and dialyzing the mixed liquor, performing freeze drying on the mixed liquor to obtain a partial aldehyde glucan solid; dissolving the partial aldehyde glucan solid in deionized water, adding a cyclohexane solution with a dissolved emulsifier, adding an amine crosslinker and stirring the solution, finally collecting precipitate through centrifugation, cleaning the precipitate to obtain the chemical cross-linking type glucan hydrogel microspheres; adding sodium borohydride in the chemical cross-linking type glucan hydrogel microspheres dispersed in deionized water, stirring the material for 24 hours under room temperature, finally collecting the precipitate through centrifugation, and cleaning the precipitate by the deionized water to obtain the reduced glucan hydrogel microspheres. According to the invention, a water-in-oil anti-phase microemulsion system is taken as a reaction medium, and the glucan hydrogel microspheres with a controllable particle size and good stability can be prepared.

Description

technical field [0001] The invention relates to a dextran-based hydrogel microsphere and a preparation method thereof, belonging to the technical field of biomedical polymer materials. Background technique [0002] Hydrogel is a three-dimensional network structure formed by physical or chemical cross-linking of polymers, which can swell in aqueous solution and retain a large amount of water without dissolving, and hydrophilic small molecules can diffuse in the hydrogel. Hydrogel microspheres have both properties of hydrogel and micro / nanoparticles. Due to its small size, large specific surface area, etc., as well as the dimensional stability and high solution absorption performance of hydrogels, hydrogel micro / nanoparticles are used as biomedical materials in tissue engineering, drug delivery, etc. and other fields have great application potential and broad application prospects (The development of microgels / nanogels for drug delivery applications, Progress in Polymer Scien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08J3/12C08J7/12C08B37/02A61K47/36
CPCC08B37/0009C08J3/075C08J3/12C08J3/24C08J7/12C08J2305/02
Inventor 苏红莹张稳陶森林张莹莹贾庆明陕绍云
Owner KUNMING UNIV OF SCI & TECH
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