937 results about "Dextran" patented technology

The invention belongs to the technical field of gene engineering, and particularly relates to applications of OpgG and a mutant thereof in production of human milk oligosaccharide. The invention provides a dextran biosynthetic protein OpgG mutant, and the OpgG mutant is obtained by mutating the 443rd amino acid from threonine to proline on the basis of a wild type dextran biosynthetic protein OpgG as shown in SEQ ID NO.1. The invention also provides a preparation method of the dextran biosynthetic protein OpgG mutant. An opgG gene and / or a T443P mutant coding gene are / is introduced into a human milk oligosaccharide production strain and applied to production of human milk oligosaccharide, and the yield of 3 '-SL, 6'-SL, LNT II, LNnT and LNT is greatly increased.

The invention discloses multifunctional hydrogel and application thereof, and belongs to the technical field of biological medicine. According to the multifunctional hydrogel, ketal mercaptan with two hydrazide ends and oxidized dextran are cross-linked to form a cross-linked network containing dynamic covalent bonds. The preparation method comprises the following steps: dissolving ketal mercaptan with two hydrazide ends in deionized water to obtain a ketal mercaptan solution, dissolving oxidized dextran in deionized water to obtain an oxidized dextran solution, mixing the ketal mercaptan solution with the oxidized dextran solution, and standing to obtain the multifunctional hydrogel. The multifunctional hydrogel has pH / ROS dual responsiveness, can respond to inflammation microenvironment and low pH of infectious wounds, remove active oxygen, release therapeutic drugs, regulate macrophage polarization and relieve bacterial infection, thereby playing a key role in inflammation, proliferation and remodeling stages of wound healing to accelerate wound healing. The curcumin-encapsulated liposome is added into the multifunctional hydrogel, so that the antibacterial effect can be further improved.

The invention relates to a composite hydrogel dressing for promoting wound healing as well as preparation and application thereof, and belongs to the technical field of biological medicines. The hydrogel disclosed by the invention is prepared by mixing gelatin, oxidized dextran and metal ion modified metal-organic framework compounds (MOF-Ag and MOF-Cu). In the mixed solution, amino groups in the gelatin and aldehyde groups of the oxidized dextran are subjected to a Schiff base cross-linking reaction, and the composite hydrogel is formed after standing. The loaded MOF-Ag has antibacterial and anti-biofilm effects on staphylococcus aureus and escherichia coli, and the loaded MOF-Cu has similar superoxide dismutase activity and similar glutathione peroxidase activity, so that excessive active oxygen can be removed, and inflammatory response can be relieved. The obtained hydrogel has good biocompatibility, proper mechanical properties and biological adhesion, stable rheological properties, excellent antibacterial and anti-inflammatory functions and a certain hemostatic function.

The invention discloses double-layer hydrogel for wound repair and infection monitoring as well as a preparation method and application of the double-layer hydrogel, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: (1) firstly, preparing an aldehyde dextran-mulberry extract solution; (2) preparing a chitosan quaternary ammonium salt-mulberry extract solution; (3) mixing and dissolving sodium alginate, aldehyde dextran and mulberry extract in a PBS (Phosphate Buffer Solution), so as to obtain an upper-layer hydrogel pre-polymerization solution; (4) mixing the aldehyde dextran-mulberry extract solution with the chitosan quaternary ammonium salt-mulberry extract solution, so as to prepare a lower layer hydrogel; and (5) adding the upper-layer hydrogel prepolymerization liquid above the lower-layer hydrogel, and spraying a metal cation salt solution to enable the upper-layer hydrogel to be fully crosslinked, thereby obtaining the crosslinked hydrogel with a double-layer structure. The double-layer hydrogel for wound repair and infection monitoring, prepared by the invention, has good adhesion, tensile property, antibacterial property and hemostatic property.

The invention discloses photocuring hydrogel capable of being combined with cytokines and used for tumor organoid culture and application, and belongs to the technical field of organoid culture. According to the invention, the hydrogel which can fix cells, can be combined with cell factors and can realize organoid recovery is developed through norbornene modified heparin, norbornene modified gelatin, sulfydryl four-arm polyethylene glycol, glucan, matrix metal protein peptidomimetic MMP and a photoinitiator. The hydrogel can be used for directly preparing organoids, heparin of various structures can be used, the cost is reduced, the modified heparin can bind factors or matrix protein and can controllably release the factors, the modified gelatin provides amino acid energy for collagen hydrolysate, and the hydrogel is formed by photocuring in a stepwise polymerization mode, is controllable in structure and can be used for preparing collagen collagen. The adjustable bionic modulus and the adjustable bionic stress relaxation are realized. The hydrogel disclosed by the invention can be used for culturing various organs, overcomes the problems of low repeatability, instability and the like in the prior art, and is suitable for the fields of tumor drug evaluation and the like.

The invention discloses a self-healing injectable photo-crosslinking dual-network hydrogel and a preparation method thereof.The preparation method comprises the steps that silk fibroin is subjected to methacrylation modification through a glycidyl methacrylate solution, then dextran of the silk fibroin is subjected to an oxidation reaction through a sodium periodate solution, oxidized dextran is generated, and the self-healing injectable photo-crosslinking dual-network hydrogel is obtained; the amino group in the modified silk fibroin and the aldehyde group of the oxidized dextran are subjected to a Schiff base cross-linking reaction to form the double-network hydrogel. The hydrogel with the double-network structure prepared by the invention is more compact in structure, so that the hydrogel has a series of excellent performances such as injectability, relatively good adhesion, good mechanical property, self-healing property and slow release property after drug loading while having safety.

The application of the animal bifidobacterium subsp. Lactis BX-245 in relieving the inflammatory bowel disease is characterized in that the animal bifidobacterium subsp. Lactis BX-245 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.26973. The invention also discloses an application of the animal bifidobacterium subsp. Lactis BX-245 in relieving the inflammatory bowel disease. The research aims at discussing the relieving effect of the animal bifidobacterium subsp. Lactis BX-245 on IBD mice induced by dextran sodium sulfate (DSS), and 6-week male C57BL / 6J mice which normally eat and drink water are randomly divided into a normal group (NC group), a model group (IBD group), a common yoghourt (FM group), an animal bifidobacterium subsp. Lactis BX-245 bacterial powder group (BX-245 group) and BX-245 yoghourt (BFM group). The relieving effect of the animal bifidobacterium subsp. Lactis BX-245 and the fermented milk of the animal bifidobacterium subsp. Lactis BX-245 on the symptom of the mouse inflammatory bowel disease is evaluated through weight change, immune factors, targeted metabonomics and metagenomics, and data support is provided for application of the animal bifidobacterium subsp. Lactis BX-245 in products.

The invention discloses a method for detecting the residual quantity of dextran sulfate sodium in an antibody. Specifically, the invention provides a method for detecting the residual quantity of dextran sulfate sodium in an antibody, which comprises the following steps: removing protein in the antibody, and reacting with a 2-aminopyridine solution; a high performance liquid chromatography method is adopted to detect a test sample and sodium dextran sulfate standard substances with different concentrations. The detection method disclosed by the invention is low in detection limit, high in accuracy and suitable for analyzing the residual quantity of dextran sulfate in the antibody.

The invention relates to injectable hydrogel for rapid hemostasis and antibiosis of an oral cavity as well as a preparation method and application of the injectable hydrogel, and the injectable hydrogel comprises the following components in percentage by weight: 5-15% of aminated chondroitin sulfate, 5-15% of oxidized dextran, 1-3% of platelet-rich fibrin and 0.5-1% of quercetin modified zeolite imidazole acid framework nanoparticles. According to the invention, Chs-ADH and ODex are adopted as matrix materials, PRF and ZIF-8 (at) QCT are introduced, the hydrogel is endowed with antibacterial, anti-oxidation and immunoregulation functions, and by optimizing the proportion of the components, the obtained hydrogel has good injectability, stability and degradation resistance, so that the hydrogel can still maintain an effective blood coagulation function in a high-humidity and high-enzymatic-activity oral environment.

The invention relates to the technical field of microorganisms, in particular to application of bacillus glucans and a complex microbial inoculant thereof in preparation of bio-organic fertilizers, the strain Mh85 is separated from the root system of apple rootstock Malus hupehensis treated by a research group through trichoderma TB2, has good IAA production, nitrogen fixation, inorganic phosphorus decomposition and siderophore production capacities, and can be used for preparing bio-organic fertilizers. The strain Mh85 not only can directly promote plant growth, but also has a good growth-promoting effect on trichoderma TB2, and when the strain is compounded with the trichoderma TB2 to form a complex microbial inoculant, tomato seedling pot experiments are carried out. The complex microbial inoculant shows a synergistic effect, effectively overcomes the problems of unstable growth promoting effect, insufficient synergistic effect among strains and the like of an existing single microbial preparation, provides an efficient and stable technical scheme for improving plant growth, improving root development and increasing crop yield, and is a good raw material for preparing a bio-organic fertilizer.

A skin cleansing formulation is provided, comprising: a dermatologically acceptable vehicle; a dermatologically acceptable oil; and a deposition aid polymer, wherein the deposition aid polymer is a cationic dextran polymer, comprising a dextran polymer functionalized with quaternary ammonium groups; wherein the deposition aid polymer enhances the deposition of the dermatologically acceptable oil from the skin cleansing formulation onto mammalian skin.

The invention belongs to the field of biology, and discloses a CRISPR / Cas12a freeze-dried microsphere, which is characterized in that the CRISPR / Cas12a freeze-dried microsphere is obtained by freeze-drying the following raw materials: enzyme-free water, 10 * HOLMES Buffer for Cas12a, an RNA enzyme inhibitor, CRISPR / Cas12a protein, crRNA, an upstream primer F, a downstream primer R, a fluorescent probe Reporter and a freeze-drying protective additive; the CRISPR / Cas12a protein is an LbaCas12a protein, and the CRISPR / Cas12a protein is a Cas12a protein; the freeze-drying protective agent is prepared from mannitol, trehalose, polyethylene glycol, glucan and BSA (Bovine Serum Albumin). By further optimizing a freeze-drying protective agent, freeze-drying microballing of a CRISPR / Cas12a system is achieved, the freeze-drying microballing can be matched with RPA constant-temperature amplification double microballing, and detection is conducted through a one-step method. Meanwhile, the invention further discloses a three-microsphere detection system, application and a kit.

The invention relates to a medicine for repairing skin wounds and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from, by mass, 25-30 parts of bee embryo peptide, 8-10 parts of alkannin derivative, 10-15 parts of armillaria mellea polysaccharide, 8-10 parts of inonotus obliquus polysaccharide, 6-8 parts of notoginsenoside and 5-7 parts of astragaloside. The bee fetus peptide is a product which is more than 500Da and is obtained by performing step-by-step enzymolysis on bee larva freeze-dried powder through trypsin and bacillus subtilis neutral protease to obtain enzymatic hydrolysate which is less than 3kDa, performing purification through a G-50 sephadex column, performing ultrafiltration and performing freeze-drying. The alkannin derivative is obtained by reacting alkannin with succinic anhydride and glucosamine-polyethylene glycol. A special method is adopted for preparing the bee embryo peptide and alkannin derivatives, the bee embryo peptide and alkannin derivatives are matched with other components to play a synergistic role, and the effects of high safety, high skin cell proliferation promoting capacity, excellent antibacterial performance and the like are achieved.

The invention discloses an inflammatory bowel disease model, a construction method and application thereof and a drug evaluation method based on the inflammatory bowel disease model, and belongs to the technical field of inflammatory bowel disease model construction. A highly bionic intestinal crypt-villus three-dimensional structure is formed based on self-organization of an intestinal cell line C2BBe1, the intestinal crypt-villus three-dimensional structure and a DMEM complete medium containing dextran sulfate sodium are added into an upper chamber of a double-layer culture chamber Transwell, the DMEM complete medium is added into a lower chamber of the double-layer culture chamber Transwell, culture is performed for 2 days, and the inflammatory bowel disease model is obtained. The method takes effect quickly and is highly simulated, the obtained model can simulate the space-time dynamic development process of intestinal inflammation molecular characteristics, and the natural recovery model and the drug treatment model constructed based on the model can provide a brand new platform for researching an inflammation regression mechanism and a drug intervention effect.

The invention relates to the technical field of immune carrier microspheres, in particular to an immune carrier microsphere constructed by individualized MHC-II combined polypeptide, a vaccine and application of the immune carrier microsphere and the vaccine. A core comprises microspheres constructed by a plurality of MHC-II binding polypeptides, sequences of the MHC-II binding polypeptides are obtained based on HLA gene typing results of an inoculator, and each HLA gene corresponds to at least one high-affinity MHC-II binding polypeptide; a shell is a glucan with sulfydryl or other polymer coating layer, and sulfydryl is used as a coupling group to be connected with an antigen to construct the vaccine. The method has the advantages that competition is avoided, inhibition is generated, a better immune effect is achieved, antibodies of high-titer carrier molecules are not generated, and cross reaction side effects are avoided. The carrier molecular diversity is reduced; side effects are avoided. And antibody immunity and cellular immunity functions are generated. The particle size of the microspheres is controllable, and the immunologic function can be achieved without adjuvants. The preparation process is simple to operate and good in repeatability.

The invention belongs to the technical field of hydrogel preparation, and particularly discloses a preparation method of CS-Pr / Odex / EGCG composite hydrogel, the hydrogel is prepared by compounding proline modified chitosan CS-Pr, oxidized dextran and EGCG, so that the prepared hydrogel not only has good injectable performance, wound healing performance and biocompatibility, but also has good biocompatibility, and can be used for preparing the hydrogel. And excellent anti-oxidation and anti-bacterial capabilities are also realized.

The invention discloses a self-crosslinking hydrogel with a tertiary amine group and preparation and application thereof, and belongs to the field of biomedical polymer materials, the self-crosslinking hydrogel comprises aminophenylboronic acid modified oxidized glucan, tertiary amine compound modified oxidized glucan and a solvent medium; the preparation method comprises the following steps: dissolving amino phenylboronic acid modified oxidized dextran and tertiary amine compound modified oxidized dextran in a solvent medium, mixing, standing for a certain time, and carrying out self-crosslinking to obtain the self-crosslinking hydrogel with tertiary amine groups. A cross-linking point (phenylboronic acid) of the gel network is a response unit of glucose. In addition, the hydrogel can effectively avoid uncontrollable drug release caused by non-responsive factors, and the response release efficiency of the drug is improved; by loading the hypoglycemic drug insulin to be applied to the treatment research process of diabetes, the occurrence of hypoglycemia can be avoided, and meanwhile, the possibility is provided for realizing intelligent controlled release of insulin through blood glucose response.

The invention belongs to the field of compositions of biomedical materials and high-molecular compounds, and provides hydrogel capable of regulating and controlling the redox steady state of a microenvironment as well as a preparation method and application of the hydrogel. A macromolecular network of the hydrogel capable of regulating and controlling the redox steady state of the microenvironment is formed by catechol modified hyaluronic acid, carboxylated chitosan, oxidized dextran and iron ions through a hybridization cross-linking reaction; under physiological conditions, catechol groups in the hydrogel can be subjected to an autoxidation reaction to generate H2O2, and meanwhile, iron ions coordinated on the catechol groups in the hydrogel can catalytically decompose H2O2 into water and oxygen, so that the level of active oxygen in a microenvironment is adjusted. The hydrogel disclosed by the invention can realize bidirectional regulation and control on active oxygen in a pathological microenvironment after myocardial infarction, so that the redox steady state of the pathological microenvironment after myocardial infarction is maintained, and the regeneration and repair capacity of an existing hydrogel material with antioxidant capacity on myocardial tissues after myocardial infarction is improved.

The invention relates to the technical field of microbial medicines, and particularly discloses Brawler's bacteria EMF75-02B1B and an application of the Brawler's bacteria EMF75-02B1B. The invention discloses Blauria sp. EMF75-02B1B, which is preserved in the Guangdong Microbial Culture Collection Center on September 2, 2024, and the preservation number of the Blauria sp. EMF75-02B1B is GDMCC No: 65084. A mouse model proves that the Brawler bacteria EMF75-02B1B can obviously improve the disease activity index (DAI) of a dextran sodium sulfate induced ulcerative colitis mouse, relieve the weight loss and colon shortening degree of the ulcerative colitis mouse, enhance the intestinal barrier function and relieve inflammatory response.

The invention relates to the technical field of breathable adhesive tapes, in particular to a double-layer composite high-breathability low-sensitivity adhesive tape and a preparation method thereof. The adhesive tape comprises a hydrophobic surface layer material, a high-breathability low-sensitivity composite adhesive and release paper. The high-breathability and low-sensitivity adhesive tape comprises a three-dimensional net-shaped breathable layer and a low-sensitivity adhesive layer. The hydrophobic surface layer material adopts a polyvinylidene fluoride electrostatic spinning nanofiber membrane with high specific surface area and porosity, so that the hydrophobicity and the adhesion with the lower layer material are improved; according to the high-breathability and low-sensitivity adhesive tape, polycaprolactone, a polylactic acid-glycolic acid copolymer and polyurethane are copolymerized to obtain a multifunctional three-dimensional net-shaped breathable layer; the high-breathability and low-sensitivity composite adhesive tape is prepared from dopamine modified polydimethylsiloxane microfibers decorated by oxidized dextran modified vinyl siloxane tip fibers, and the adhesive force and biocompatibility of the adhesive tape and skins with different roughness degrees are enhanced. Meanwhile, the lignosulfonate surfactant is added, so that the reproduction of bacteria and fungi in an adhesive tape attachment area can be inhibited.

The invention relates to efficient anti-dandruff shampoo and a preparation method thereof, and belongs to the technical field of shampoo. The efficient anti-dandruff shampoo comprises the following raw materials: 0.7%-1% of a plant extract, 0.04% of asiatic acid, 30%-35% of a humectant, 7%-10% of a surfactant, 18% of sodium propionate, 2%-4% of a thickener, 0.2%-0.3% of an emulsifier, 0.8%-1% of an acidity regulator, 0.05% of tremella polysaccharide, 2%-3% of dextran enzyme, 0.3%-0.5% of a preservative, 2%-3% of a conditioner, 0.5% of isethionate and the balance of deionized water. Three effective components including lophatherum gracile ketone, cacumen biotae extract and cnidium monnieri extract are prepared into microcapsules to be added into the shampoo, the microcapsules are mixed with asiatic acid and an anti-dandruff component isethionate, and through the combined action of all the components, the purpose of effectively removing dandruff is achieved.

The invention relates to the technical field of microbial medicines, and in particular discloses a doer's bacterium EMF18-06B1D and an application of the doer's bacterium EMF18-06B1D. The invention relates to a Dorenia phocaeensis EMF18-06B1D, which is preserved in Guangdong Microbial Culture Collection Center on September 2, 2024, and the preservation number of the Dorenia phocaeensis EMF18-06B1D is GDMCC No: 65085. The invention further discloses a preparation method of the Dorenia phocaeensis EMF18-06B1D. A mouse model proves that the polythermia EMF18-06B1D can obviously improve the disease activity index (DAI) of a dextran sodium sulfate induced ulcerative colitis mouse, relieve the weight reduction trend and the colon shortening degree of the ulcerative colitis mouse, and contribute to intestinal barrier function recovery and intestinal inflammatory response reduction.

The invention belongs to the technical field of biology, and particularly relates to a high-throughput preparation method of a single-cell-derived organoid based on a microcapsule spherical shell structure, and the preparation method comprises the following steps: preparing an aqueous two-phase microcapsule encapsulated with a single cell, culturing the single cell in the aqueous two-phase microcapsule to obtain the organoid, the substances of the inner phase and / or the outer phase respectively comprise any one or a combination of more of the following substances: methacrylated gelatin, carboxymethyl cellulose, glucan, sodium alginate and polyvinyl alcohol. The invention also provides the organoid prepared by the preparation method and application of the preparation method. According to the invention, the microcapsule with a permeable core-shell structure is formed by packaging the microcapsule in a single-cell form in two-aqueous-phase liquid drops, the wrapping rate is effectively controlled and adjusted on the premise of conforming to Poisson distribution by optimizing parameters, and the single-cell wrapping rate and the organoid single-cell induction efficiency are optimized.

The invention relates to the field of animal consumer goods, and particularly discloses application of glucan in preparation of animal products for relieving pet stress syndromes. Pet experiments find that beta-1, 3 / alpha-1, 3-glucan can improve the stress syndromes of the indoor cats, specifically, abnormal behaviors of the cats are corrected, and abnormity of multiple systems of organisms such as the immune system, the endocrine system and the urinary system is improved. And when the method is applied to pets, the life quality of the pets can be remarkably improved, more emotional support is provided for pet owners, and the method has practical popularization and application values.

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.

The invention discloses a preparation method of compound polysaccharide microspheres capable of releasing NO. The preparation method comprises the following steps: cross-linking swallow polysaccharide with L-ARG, and preparing L-ARG-containing swallow polysaccharide hydrogel through a Mannich reaction; then loading EGCG (epigallocatechin gallate) onto the L-ARG-containing swallow polysaccharide hydrogel through a Schiff base reaction, and obtaining the compound polysaccharide microspheres capable of releasing NO by utilizing an emulsification method. The invention also discloses an application thereof. The hydrogel microsphere capable of targeting the colon is prepared, and the hydrogel microsphere can be accurately exploded at the colon part after being swallowed. A dextran sodium sulfate (DSS)-induced IBD mouse model is established to test the drug effect, and the result shows that the compound polysaccharide microspheres have a remarkable treatment effect on DSS-induced ulcerative colitis, can obviously improve the symptoms of ulcerative colitis, and provides a theoretical basis and a clinical basis for new drugs for clinically preventing and treating ulcerative colitis.

The invention relates to the technical field of preparation of biological materials and pharmaceutical preparations, in particular to organic composite hydrogel as well as a preparation method and application thereof. The specific preparation method comprises the following steps: dissolving a targeted drug in orthoester to obtain a targeted drug-orthoester solution; the preparation method comprises the following steps: mixing an oxidized dextran solution, a targeted drug-orthoester solution and a carboxymethyl chitosan solution, carrying out vortex treatment, and standing to prepare the organic composite hydrogel. The solubility of the targeted drug in the hydrogel is improved by utilizing orthoester, the orthoester can carry the targeted drug to efficiently escape from the hydrogel, and the targeted drug can be self-assembled to form nanoparticles along with degradation of the orthoester in the escape process, so that the retention capacity of the targeted drug at a tumor site is enhanced, and the targeting drug can be used for treating tumor tumors. The drug enrichment capacity is improved, meanwhile, the toxic and side effects of the whole body are reduced, and the problems that nano-drugs are poor in solubility, still prone to being removed after intravenous administration and poor in enrichment effect at tumor sites are solved.

The invention discloses hydrogel for repairing volumetric muscle defects and a preparation method of the hydrogel, and the hydrogel is prepared from oxidized dextran, an alcohol-polyamino acid-bilirubin polymer and 3, 3 '-dithio-bis (propionyl hydrazide) according to the weight ratio of (50-75): (4-20): 1. The alcohol-polyamino acid-bilirubin polymer is prepared from an alcohol-polyamino acid polymer and bilirubin in a weight ratio of (6-25): 1. The hydrogel prepared by the invention not only has good characteristics of pH / ROS responsiveness, relatively strong tissue adhesion, antibacterial property, oxidation resistance and relatively low cytotoxicity, but also can remarkably promote cell migration, proliferation, new angiogenesis and myoblast differentiation, promote recovery of muscle structures and motor functions after volumetric muscle defects, and improve the recovery rate of muscle tissues and motor functions after the volumetric muscle defects. The material is expected to become a novel bioactive material capable of promoting volume muscle defect repair, and has a good application prospect in clinical treatment of large-volume tissue defect regeneration.

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention discloses injectable hydrogel for alveolar bone repair, the injectable hydrogel comprises a first component and a second component, the first component comprises 4-carboxyl-3-fluorophenylboronic acid modified carboxymethyl chitosan CMCS-FPBA and beta-tricalcium phosphate, and the second component comprises oxidized dextran, PPS-HA nano-micelle and curcumin. The invention also discloses a preparation method and application of the injectable hydrogel. The injectable hydrogel disclosed by the invention has the effects of resisting oxidation, resisting inflammation and promoting bone tissue regeneration, is high in biocompatibility, can be customized in a personalized manner, and can be used for repairing alveolar bones.