387 results about "Dextran" patented technology

The invention belongs to the field of biology, and discloses a CRISPR / Cas12a freeze-dried microsphere, which is characterized in that the CRISPR / Cas12a freeze-dried microsphere is obtained by freeze-drying the following raw materials: enzyme-free water, 10 * HOLMES Buffer for Cas12a, an RNA enzyme inhibitor, CRISPR / Cas12a protein, crRNA, an upstream primer F, a downstream primer R, a fluorescent probe Reporter and a freeze-drying protective additive; the CRISPR / Cas12a protein is an LbaCas12a protein, and the CRISPR / Cas12a protein is a Cas12a protein; the freeze-drying protective agent is prepared from mannitol, trehalose, polyethylene glycol, glucan and BSA (Bovine Serum Albumin). By further optimizing a freeze-drying protective agent, freeze-drying microballing of a CRISPR / Cas12a system is achieved, the freeze-drying microballing can be matched with RPA constant-temperature amplification double microballing, and detection is conducted through a one-step method. Meanwhile, the invention further discloses a three-microsphere detection system, application and a kit.

The invention belongs to the technical field of biology, and particularly relates to a high-throughput preparation method of a single-cell-derived organoid based on a microcapsule spherical shell structure, and the preparation method comprises the following steps: preparing an aqueous two-phase microcapsule encapsulated with a single cell, culturing the single cell in the aqueous two-phase microcapsule to obtain the organoid, the substances of the inner phase and / or the outer phase respectively comprise any one or a combination of more of the following substances: methacrylated gelatin, carboxymethyl cellulose, glucan, sodium alginate and polyvinyl alcohol. The invention also provides the organoid prepared by the preparation method and application of the preparation method. According to the invention, the microcapsule with a permeable core-shell structure is formed by packaging the microcapsule in a single-cell form in two-aqueous-phase liquid drops, the wrapping rate is effectively controlled and adjusted on the premise of conforming to Poisson distribution by optimizing parameters, and the single-cell wrapping rate and the organoid single-cell induction efficiency are optimized.

The invention relates to the field of animal consumer goods, and particularly discloses application of glucan in preparation of animal products for relieving pet stress syndromes. Pet experiments find that beta-1, 3 / alpha-1, 3-glucan can improve the stress syndromes of the indoor cats, specifically, abnormal behaviors of the cats are corrected, and abnormity of multiple systems of organisms such as the immune system, the endocrine system and the urinary system is improved. And when the method is applied to pets, the life quality of the pets can be remarkably improved, more emotional support is provided for pet owners, and the method has practical popularization and application values.

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.

The invention relates to a medicine for skin regeneration and repair and a preparation method thereof, and belongs to the technical field of biological medicine, the medicine comprises cyperus esculentus extract peptide, brooklet anemone root tremella extract, rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract, pomegranate peel polyphenol and the like. The cyperus esculentus extracted peptide is a product which is more than 1kDa and is obtained by performing enzymolysis on cyperus esculentus through cellulase, alpha-chymotrypsin and ficin protease in sequence and then performing purification through a G-50 sephadex column; the tremellodon gelatinosum tremella extract is a product which is less than 3kDa and is obtained by performing common enzymolysis on tremellodon gelatinosum tremella sporocarp through cellulase and bromelain; the rhodomyrtus tomentosa root-suberect spatholobus stem-radix cyathulae composite extract is a product obtained by performing gradient extraction on rhodomyrtus tomentosa root, suberect spatholobus stem and radix cyathulae through an ethanol water solution to obtain an extract, and performing polyamide resin column adsorption and LH-60 sephadex column purification in sequence. A good repairing system is constructed by multiple components, and wound repairing and healing are accelerated.

The invention discloses application of copper-amino acid nano enzyme in preparation of anti-inflammatory drugs, and relates to the technical field of biomedical new materials, amino acids comprise glycine, arginine, histidine, threonine, phenylalanine and cysteine; the ratio of the amino acid to the copper ions is 1: (0.5-5), and the synthesis temperature of the copper-cysteine nano enzyme is 25-125 DEG C. The copper-amino acid nano-enzyme library established by the invention has efficient hydroxyl free radical, superoxide free radical and hydrogen peroxide scavenging activity; wherein the copper-cysteine nano-enzyme shows the highest enzymatic activity, and shows low toxicity and good biocompatibility in both the cell level and the animal level; meanwhile, the copper-cysteine nano-enzyme also shows anti-inflammatory activity and anti-oxidative stress activity, can remarkably improve cell inflammation and body inflammation, relieves and treats dextran sodium sulfate induced mouse ulcerative colitis, and can be further applied to preparation of drugs for treating inflammatory bowel diseases.

The present application relates to a kind of pH / H2O2 dual-response drug-loaded hydrogel and its preparation method and application, by ε-polylysine derivative as antitumor active ingredient, with oxidized dextran through Schiff base reaction crosslinking rapid gelation. While using 2-formylphenyl boronic acid covalently package CD73 small molecule inhibitor APCP. ε-polylysine derivative has oncolytic activity, can induce ICD and promote the infiltration of immune cells in tumor cells. CD73 small molecule inhibitor APCP can block extracellular CD39-CD73-adenosine pathway, reduce the level of immunosuppressive metabolite adenosine in tumor tissue, so as to activate the infiltrated immune cells, restore its anti-tumor function. The hydrogel of ε-polylysine derivative and APCP co-delivery synergistically inhibits the effect of tumor growth, and its curative effect is greater than the treatment effect of single drug, and is superior to positive control drug oxaliplatin (OXA). The pH / H2O2 dual-response hydrogel is rapidly gelled, and has good biological safety under mild reaction conditions.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.

The invention discloses a construction method and application of a probiotic engineering bacterium EcNpAm targeting animal intestinal tracts and improving inflammation. According to the present invention, the heterologous expression of the immunomodulatory protein Amuc1100 is successfully achieved through the gene engineering technology by using the Escherichia coli Niss1917 (EcN) as the host and using the anaerobic inducible plasmid pnirBMisL as the expression vector, and the novel engineering bacterium EcNpAm suitable for the animal intestinal anaerobic environment is constructed; furthermore, the expression efficiency of the target protein is represented by fusion expression of green fluorescent protein (GFP). The engineering bacterium is applied to prevention and treatment of animal intestinal inflammation, and successfully realizes targeted delivery of therapeutic protein to intestinal tracts of mice. Experiments show that EcNpAm can effectively relieve typical symptoms such as weight loss, colon shortening, pathological injury, colitis and the like of mice induced by dextran sodium sulfate (DSS) and protect integrity and functions of an intestinal barrier structure.

Surfactant compositions comprising a reaction product of a saccharide polymer and a fatty acid or a fatty ester may be obtained in an aqueous fluid in the presence of a hydroxide base and a neutral surfactant or a reaction product form thereof, the saccharide polymer comprising a dextran, a dextrin compound, or any combination thereof. One or more zwitterionic surfactants may be combined with the surfactant composition in a ratio sufficient to render the resulting combined surfactant composition brine-tolerant, even when both the reaction product and one or more of the zwitterionic surfactant(s) are brine-intolerant. The one or more zwitterionic surfactants may be further selected to render the combined surfactant composition non-emulsifying toward oleaginous fluids.

The invention relates to the technical field of graded screening, in particular to a graded screening system based on staggered stacked metal ring films and a preparation method and application. An organic ligand and two metal complexes are subjected to interface coordination assembly under the action of silver ions, an ordered staggered stacked metal ring film is constructed, the structure and performance of the metal ring film are precisely regulated and controlled, the metal ring film has a definite molecular sieve component difference, and then precise grading screening of different molecules is achieved. The film has an obvious lattice structure, has good crystalline orderliness, and can ensure the stability and reliability of the screening process. The graded screening system based on the staggered stacked metal ring membranes has excellent interception performance on target molecules, and effective separation of TAC from glucan and leucine can be achieved. The two-stage membrane classification screening strategy can also be popularized to separation and purification of other substances, and good universality and industrial application prospects are achieved.

The invention discloses a piglet intestinal health targeting probiotic engineering bacterium compound as well as preparation and application thereof. The compound is FP (at) EcN-pnirNKL, wherein a) the EcN-pnirNKL is an engineering strain which is constructed by taking escherichia coli Niss1917 (EcN) as a host and carrying a recombinant plasmid pnirBMisL-NK-Lysin, and a) the EcN-pnirNKL is an engineering strain which is constructed by taking escherichia coli Niss1917 (EcN) as a host and carrying a recombinant plasmid pnirBMisL-NK-Lysin; b) the recombinant plasmid pnirBMisL-NK-Lysin comprises an NK-Lysin gene optimized by a codon, and the NK-Lysin gene is integrated into the anaerobic inducible plasmid pnirBMisL through homologous recombination; c) the FP is a composite nano-particle which takes nano mesoporous silica (MSN) as a core and is formed by crosslinking fucoidin and rhodiola rosea polysaccharide; and d) the FP (at) EcN-pnirNKL is an oral delivery system which is formed by encapsulating the engineering bacterium EcN-pnirNKL in the FP nano particles. When the compound is applied to prevention and treatment of stress response of weaned piglets, various typical symptoms, including weight loss, colon shortening, histopathological injury and colitis disease response, of the piglets induced by dextran sodium sulfate can be remarkably relieved, and meanwhile, the integrity and functions of an intestinal barrier structure are effectively protected.

The invention belongs to the technical field of immunology, and discloses a manganese salt adjuvant system based on dextran particle capsule shrinkage and a preparation method and application thereof.The manganese salt adjuvant system comprises beta-dextran particles GPs of a hollow spherical shell structure from yeast and manganese hydroxide colloid encapsulated in the dextran particles, the preparation method comprises the following steps: soaking the GPs in a MnCl2 solution, after the inner cavity of the GPs fully absorbs the MnCl2 solution, separating the GPs adsorbing MnCl2, then adding the GPs into a NaOH solution, forming stable manganese hydroxide colloid by manganese ions in the inner cavity of the GPs, and washing after separation to obtain the dextran particle encapsulated manganese salt adjuvant system. The GPMnOH particles carry various antigens at the same time in various modes such as electrostatic adsorption, chemical coupling or hydrophobic interaction, and an ideal carrier is provided for co-delivery immunization of the antigens and adjuvants.

The present invention relates to a hollow fiber membrane comprising at least one polysulfone-based material and at least one polyvinylpyrrolidone-based polymer, characterized in that the porosity of the hollow fiber membrane is from 77.5% to 82% and the sieving coefficient for dextran with a molecular weight of 10,000 g / mol is from 0.42 to 0.75 and the albumin sieving coefficient of the hollow fiber membrane is less than 0.01.

The invention belongs to the technical field of biomedical materials, and discloses a viola philippica exosome-loaded composite hydrogel as well as a preparation method and application thereof, the composite hydrogel is prepared by mixing methacrylated polylysine (PLMA), oxidized dextran (ODex), viola philippica exosome (V-E), a photoinitiator and a physiologically compatible solvent and performing illumination crosslinking; the preparation method comprises the following steps: respectively preparing PLMA and ODex solutions, extracting and purifying V-E, adding a photo-crosslinking agent (LAP), mixing the components to obtain a precursor liquid, and carrying out in-situ curing molding through ultraviolet-visible light irradiation. The composite hydrogel provided by the invention solves the problems that the existing wound dressing is difficult to fit with irregular wounds, is antibacterial and easy to resist drugs, and lacks active anti-inflammatory and efficient healing promotion capabilities, and is suitable for repair treatment of infectious open wounds such as burns, trauma, diabetic foot ulcer and postoperative infected incisions.

The present application belongs to the technical field of oral care, and particularly relates to an oral care composition and a preparation method thereof. The oral care composition comprises, by mass percentage, chitosan oligosaccharide 0.1-0.3%, sea hao polysaccharide 0.08-0.14%, forskolin 0.07-0.12%, corn sprout extract 0.05-0.09%, thickening agent 1-3%, humectant 40-50%, and the balance being water. The oral care composition of the present application can effectively inhibit the activity of glucosyltransferase, inhibit the production of dextran, interfere with the formation process of dental plaque, and reduce the adhesion sites of pathogenic bacteria in the oral cavity. In addition, the composition of the present application can promote the proliferation and repair of oral mucosa epithelial cells, and help repair oral mucosa damage. Furthermore, the use of each component in the composition of the present application can balance the oral microbial flora, while achieving the effect of inhibiting pathogenic bacteria and improving the oral environment.

The invention relates to the technical field of immune carriers, in particular to immune carrier microspheres loaded with individualized MHC-II binding polypeptide and vaccine preparation and application of the immune carrier microspheres loaded with the individualized MHC-II binding polypeptide. The core microsphere is loaded with individualized MHC-II binding polypeptide, the sequence of the MHC-II binding polypeptide is obtained by predicting and screening based on an HLA genotyping result, and each HLA allele corresponds to at least one high-affinity MHC-II binding polypeptide; and the shell is a glucan or other polymer coating layer. The preparation method has the advantages that the T epitope and the B epitope are separately subjected to immune competitive inhibition inside and outside the microspheres, so that a better immune effect is obtained. The antibody avoids cross reaction side effects; carrier molecule diversity is reduced, and side effects caused by T cell over-activation are avoided; th1 epitopes and Th2 epitopes can be contained in the microspheres, so that antibody immunity and T cell immunity functions are generated; the particle size of the microspheres is controllable, and the immunologic function can be achieved without adjuvants.

The invention discloses antibacterial mouthwash as well as a preparation method and application thereof, and belongs to the technical field of daily chemical products. According to the invention, egg white lysozyme, ferulic acid and grape seed procyanidine are taken as core components, and the mouthwash with an efficient bacteriostatic effect is developed. And the antibacterial rates of streptococcus mutans and porphyromonas gingivalis are 96.88% and 96.30% respectively. Researches show that bacterial biofilms can be destroyed by using the mouthwash, and the biofilm quantity is reduced by 53.7% after the bacterial biofilms are treated for 24 hours; extracellular polysaccharide is inhibited, the content of water-insoluble glucan is reduced by 62.3%, and synthesis of a key cariogenic matrix is hindered; the acid production capacity is regulated and controlled, the pH change of 24-hour culture is only 1.50, and xylitol competitively inhibits glycolysis and cooperates with ferulic acid to block bacterial metabolism. The mouthwash prepared by the invention avoids the problem of enzyme activity inhibition of the ionic surfactant of the traditional mouthwash, takes effect more quickly, and provides a basis for developing natural, safe and efficient functional mouthwash.

The invention provides diglyceride goat milk powder. The diglyceride goat milk powder is prepared from the following components in parts by weight: 700 to 800 parts of raw goat milk, 20 to 50 parts of safflower seed diester oil, 100 to 200 parts of isomaltooligosacharide, 1 to 3 parts of compound vitamins, 0.1 to 0.5 part of compound probiotics, 80 to 120 parts of whey protein isolate and 25 to 35 parts of glucan. According to the invention, high-purity safflower seed diester oil is used as a core raw material, and an innovative solution is provided for the functional goat milk powder market. By adopting a unique process of combining enzymolysis pretreatment and composite embedding, the bioavailability of functional components is remarkably improved on the basis of ensuring excellent reconstituability and storage stability of the product. The product has the dual effects of metabolic regulation and intestinal health at the same time, functions are enhanced while the natural nutritional value of the goat milk is reserved, and a new technical path is provided for development of functional dairy products.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The invention discloses granulation equipment and process for dextran iron sheets, and relates to the technical field of medicine granulation, and the granulation equipment and process are technically characterized in that the granulation equipment comprises a tank body, a main material pipe and an auxiliary material pipe are connected to the two sides of the top of the tank body respectively, the main material pipe is used for feeding dextran iron, the auxiliary material pipe is used for feeding auxiliary materials, and a transversely-arranged isolation plate is arranged at the bottom of the tank body; a partition plate is arranged in the tank body and divides the interior of the tank body into an upper stirring cavity and a lower extruding cavity, a stirring mechanism is arranged at the central axis of the stirring cavity, an automatic opening and closing mechanism is arranged on the partition plate, an extruding mechanism is arranged at one end of the extruding cavity, a discharging pipe is arranged at the other end of the extruding cavity, and a granulating screen is arranged in the discharging pipe; a material cutting mechanism is arranged on the face, away from the material extruding cavity, of the granulating screen and used for cutting off the mixture extruded out of the granulating screen. According to the invention, the automation degree can be improved, the manual labor is reduced, and the granulation convenience is improved.

The invention discloses composite hydrogel for wound repair as well as a preparation method and application of the composite hydrogel, and belongs to the technical field of medical materials. The preparation method comprises the following steps: mixing methylacryloylated gelatin, hydroxyethyl methylacrylate and oxidized dextran, adding copper sulfide nanoparticles, a cross-linking agent and a photoinitiator, uniformly stirring, placing in a mold, irradiating by adopting ultraviolet light, carrying out Schiff base reaction on an aldehyde group on an ODex chain and an amino group on a GelMA chain to form a dynamic covalent bond, and forming a reversible cross-linking point, so as to obtain the copper sulfide nanoparticle / GelMA composite hydrogel. The preparation method comprises the following steps: carrying out a copolymerization reaction on HEMA and methacryloyl on a GelMA molecular chain to form a stable covalent cross-linked network, effectively capturing CuS NPs through a physical effect and stably dispersing the CuS NPs in the network in the process, and soaking the cross-linked hydrogel in deionized water for balancing to obtain the target composite hydrogel. The composite hydrogel has excellent mechanical strength, pH response swelling property and biocompatibility, can realize efficient antibacterial and endogenous immune regulation by combining near-infrared photothermal therapy, and effectively promotes wound repair.

In specific embodiments, provided is a material for preventing adhesion of membranes in an ophthalmic surgery for treatment of glaucoma or the like without using mitomycin-C or the like. Particularly provided is such material, which is easy to handle and has no risk of histolysis or infections. In a production method of a preferred embodiment, prepared are: aldehyde-modified dextran (first reactant) having weight-average molecular weight of 10,000-5,000,000 and introduced aldehyde group-per-anhydrous glucose unit (mol / AGU) of 0.4-0.7; and succinic anhydride-added poly-L-lysine (second reactant) having weight-average molecular weight of 1000-100,000 and having residual amino group ratio of 80-99%; then, they are mixed together so that aldehyde group-to-amino group molar ratio becomes 0.9-1.1 and are reacted in presence of water so that polymer concentration becomes 8-20%; and a hydrogel produced by the reaction, or a suspension before gelation, is freeze-dried to produce a porous sheet having a thickness of 0.1-0.8 mm.

Disclosed is a composition for treating a joint disease or a connective tissue disease, a composition for cartilage regeneration, or a composition for treating an inflammatory disease, each of the compositions containing dextran, poloxamer or a mixture thereof. The composition stays in the joint or connective tissue for a long time due to the shock-absorbing effect, coating effect or anti-inflammation effect, relieves the shock, covers a damaged portion in a specific manner thereto, or reduces inflammation of an adhered portion. Thus, the composition may be useful for the treatment of the joint disease, the connective tissue disease, or for the cartilage regeneration.

Disclosed herein is method for conjugating a metal chelating agent to a functionalized dextran by reacting a chelator with an aminated dextran backbone, where the chelator comprises a one, and only one, derivatized carboxylic acid group to form a chelator-dextran complex. In certain aspects, the dextran-chelator complex is substantially free of intra- or intermolecular crosslinking. In certain aspects, the functionalized dextran is an amine dextran, an alkynyl dextran, or a thiol dextran. In exemplary implementations, the functionalized dextran is an amine dextran. In further embodiments, one and only one carboxylic acid group on the chelating agent is derivatized as a N-hydroxysuccinimide (NHS) ester.

A rizatriptan microneedle patch, a preparation method therefor, and use thereof. The rizatriptan microneedle patch comprises a substrate and a needle body arranged on a surface of the substrate. The composition of the needle body comprises rizatriptan benzoate and a skeleton material. The skeleton material comprises a combination of dextran and trehalose, copovidone, a combination of polyvinyl alcohol and dextran, a combination of hypromellose and dextran, or a combination of sodium carboxymethyl cellulose and dextran. The rizatriptan microneedle patch has high mechanical strength and cumulative drug permeation.

The application provides a preparation method and application of a near-infrared two-region fluorescent sensor, and chiral single-walled carbon nanotubes are separated by using a double water phase extraction method; materials in the separation process include deionized water, polyethylene glycol, dextran, sodium dodecyl sulfate, sodium deoxycholate and cholic acid sodium hydrate; the chiral single-walled carbon nanotubes are dispersed and mixed with 4-nitrobenzene tetrafluoroboric acid diazonium salt to obtain the fluorescent sensor; high-purity (6,5)-SWCNT is efficiently and simply separated by using the double water phase extraction technology, the prepared SWCNT-NBD nanomaterial has good optical performance, including bright light emission in the near-infrared two-region, has good penetration capacity on biological tissues, the fluorescent detector (SWCNT-NBD) has low detection line, wide linear range, good selectivity on DA, and is convenient to prepare and low in cost.

The invention belongs to the field of traditional Chinese medicine extraction and separation, and particularly relates to a heterocyclic alkaloid compound in purslane as well as an extraction and separation method and application thereof. The molecular formula of the compound is C9H12N4O5, and the chemical name of the compound is 5-(4-hydroxy-3-((hydroxymethyl) amine) isoxazolidin-5-yl)-5H-pyrrolo [3, 4-d] isoxazolidin-4-ol. The structural formula of the compound is shown in the specification, and the structural formula of the compound is shown in the specification. The invention also provides an extraction and separation method of the compound. Ethanol extraction, ODS column chromatography separation, sephadex gel chromatographic column separation and ultra-high performance liquid chromatography are adopted for separation, purification and preparation. The structure of the compound is determined as a new compound by mass spectrum, hydrogen spectrum, carbon spectrum and two-dimensional nuclear magnetic spectrum analysis methods, and the compound has anti-inflammatory, anti-cholinesterase and antioxidant activity. The heterocyclic alkaloid compound and the salt or derivative thereof can be used as raw materials for drug development and pharmacological activity research.

The present invention relates to a large scale GMP synthesis method of a modified glucan conjugate, and more particularly, to a glucan-guanidine-bisphosphonate conjugate wherein the bisphosphonate is an alendronate group. The large-scale GMP synthesis method related to the invention can be used for producing the glucan-guanidine-bisphosphonate conjugate with high purity and medicinal grade. The invention also relates to an application of the glucan-guanidine-bisphosphonate conjugate in a medicine for treating tumors.

The invention provides a pharmaceutical composition for improving bone mineral density and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises lactarius vomica extract peptide, winged yam rhizome peel extract, rainbow trout extract, calcium citrate-glycine chelate, vitamin K2 and vitamin D3. The lactarius volemus extract peptide is obtained by sequentially carrying out enzymolysis on lactarius volemus by bromelain and subtilisin and purifying and extracting enzymatic hydrolysate through an LH-60 sephadex column. The winged yam rhizome peel extract is obtained by extracting the peels of winged yam rhizome tubers with an ethanol water solution and purifying and extracting the extracting solution with an AB-8 macroporous adsorption resin column. The rainbow trout extract is a product below 5kDa obtained by sequentially performing enzymolysis on fish heads, fish skins and fish scales of rainbow trout through trypsin and lumbrukinase. All the components are compounded according to a specific proportion, and the core effect of improving the bone mineral density is achieved through two-way regulation and control of bone metabolism, optimization of calcium utilization and improvement of safety.