24 results about "Laminarin" patented technology

The invention discloses an esophageal cancer targeting nano-tablet based on a traditional Chinese medicine composition and a preparation method of the esophageal cancer targeting nano-tablet. The targeted nano tablet is prepared from the following refined extracts: a rhizoma paridis total saponin extract, a pseudobulbus cremastrae seu pleiones colchicine derivative extract, a panax notoginseng total saponin extract, a carthamin yellow extract, a thunberg fritillary bulb total alkaloid extract, frankincense-myrrh compound volatile oil, a resin extract and an astragaloside IV extract. The nano-carrier comprises a nano-carrier, a seaweed-laminarin composite extract, a processed rhizoma pinelliae total alkaloid extract and costus root volatile oil, and the costus root volatile oil serves as one of targeting ligands to be used for modifying the nano-carrier. The targeting property is strong: the dual-targeting ligand (costunolide-TOPK affinity peptide) on the surface of the nano-carrier can actively recognize and combine with a specific receptor of esophageal cancer cells, and meanwhile, due to the size (about 100-200 nanometers) of the carrier, the carrier is easy to enrich in tumor tissues through enhanced permeation and retention (EPR) effect, so that the modernization upgrading of the idea of'channel guiding medicine 'is realized.

The application belongs to the technical field of cosmetics, and discloses a fucoidan polysaccharide composition with bacteriostatic efficacy, and a preparation method and application thereof. The fucoidan polysaccharide composition comprises phaeophycus triquetrum fucoidan, small new moon rhombus fucoidan and false micro-halochain fucoidan, and the mass ratio of the phaeophycus triquetrum fucoidan, the small new moon rhombus fucoidan and the false micro-halochain fucoidan is (1-2):(1-2):(1-2). The fucoidan polysaccharide composition is prepared by compounding specific phaeophycus triquetrum fucoidan, small new moon rhombus fucoidan and false micro-halochain fucoidan, has a synergistic effect, and has excellent antibacterial efficacy. When the fucoidan polysaccharide composition is applied to cosmetics, it has good formula adaptability, does not affect the exertion of efficacy and the stability of the cosmetics.

The invention discloses a preparation method and application of ulcerative colitis resisting kelp fucoidin, and belongs to the technical field of polysaccharide preparation and application, the preparation method is used for preparing the kelp fucoidin, and the preparation method comprises the following steps: taking kelp as a raw material, cleaning, drying, crushing and sieving, and then performing reflux degreasing to obtain kelp powder; carrying out hot water-ultrasonic synergistic extraction on the algae powder, and carrying out centrifugation and rotary evaporation concentration to obtain a supernatant; performing graded alcoholization and deproteinization treatment on the supernate to obtain a deproteinized polysaccharide solution; and dialyzing the deproteinized polysaccharide solution to remove impurities, freeze-drying to obtain the kelp fucoidin, and applying the obtained kelp fucoidin to treatment of ulcerative colitis. The preparation process is simple, efficient and high in repeatability, compared with an artificially synthesized ulcerative colitis resisting medicine mesalazine, the obtained natural fucoidin has the same level of ulcerative colitis resisting activity, a new natural active raw material is provided for ulcerative colitis treatment, and the natural fucoidin has a good application prospect.

The application discloses sodium alginate-kelp polysaccharide-ganoderic acid D nanoparticles for targeted treatment of ulcerative colitis and a preparation method and application thereof. Raw materials of the nanoparticles include sodium alginate, kelp polysaccharide and ganoderic acid D with a mass ratio of 20-30:2-5:8-12. The sodium alginate-kelp polysaccharide-ganoderic acid D nanoparticles are prepared by taking sodium alginate gel as a carrier and loading kelp polysaccharide and ganoderic acid D in the carrier, and the nanoparticles are used for preventing and / or relieving ulcerative colitis, can have antioxidant effect, target regulation of intestinal barrier function, reduction of inflammatory reaction and improvement of intestinal health, and provide a new method for ulcerative colitis.

The invention discloses a method for producing chrysophyceae laminarin through high-density culture of chrysophyceae and an application of the chrysophyceae laminarin. The culture method comprises the following steps: (1) culturing a chrysophyceae species under low-temperature and low-light conditions, and then transferring the chrysophyceae species into a seed culture medium for culturing to obtain activated chrysophyceae species; (2) placing the activated algae species obtained in the step (1) under a dark condition for shake culture to obtain dark adaptation algae species; and (3) inoculating the dark adaptation algae seeds into a fermentation tank, and adding a basic culture medium and a fed-batch culture medium for fermentation culture. By selecting the nitrogen source and accurately controlling the ammonium concentration, the double improvement of the biomass and the yield of the chrysophyceae laminarin is realized, the adverse effect of the high-concentration nitrogen source on growth is avoided, and the culture efficiency and the product economy are improved.

The present application provides a kind of endo beta-1, 3-glucanase and its application, the amino acid sequence of the endo beta-1, 3-glucanase is SEQ ID NO:1.The endo beta-1, 3-glucanase of the present application can efficiently hydrolyze laminarin and curdlan, its enzyme activity can still be kept above 80% in the range of 40-60 DEG C and pH 4.5-6.0, and it shows good stability under low temperature conditions, which is conducive to reduce the energy consumption in the process of practical application.At the same time, the enzyme has significant high salt tolerance, and has certain resistance to 5%-15% ethanol.The endo beta-1, 3-glucanase of the present application has obvious inhibitory effect on the formation of Candida albicans biofilm, and the laminarin oligosaccharide generated by hydrolyzing laminarin also shows good antioxidant capacity, indicating that the enzyme has broad application prospect and significant practical value in the fields of antibacterial, biological medicine and the like.

The present disclosure relates to fungicidal combinations. The present disclosure relates to fungicidal combinations and compositions comprising laminarin and a contact fungicide for controlling fungal diseases in crops. The present disclosure further relates to a method of controlling phytopathogenic fungal diseases.

The invention relates to the technical field of biological medicines, in particular to an intestinal nutrition regulator containing laminarin and a preparation method of the intestinal nutrition regulator. The intestinal nutrition regulator containing the laminarin is prepared from the laminarin, probiotics and auxiliary materials through scientific proportioning and a unique process. The preparation method comprises the following steps: firstly, efficiently extracting polysaccharide from kelp through a process of combining ultrasonic-assisted enzyme extraction (UEE) and acid-assisted extraction (AAC); meanwhile, by exerting the synergistic effect of the laminarin, the silymarin extract and the resveratrol, the composition can effectively improve the intestinal flora structure, enhance the integrity of the intestinal barrier, inhibit the growth of pathogenic bacteria and regulate immune response. The intestinal nutrition regulator can be used for preparing food or health care products for regulating and / or improving intestinal flora balance of mammals, has good effects on preventing and improving intestinal related diseases such as intestinal dysfunction, irritable bowel syndrome and inflammatory bowel disease, and has wide market prospect and application value.

The present application belongs to the technical field of skin care products, and particularly relates to a water-light needle skin care composition. A preservative composition is composed of p-hydroxyacetophenone and laminarin at a mass ratio of 1:3. When p-hydroxyacetophenone and laminarin are used at a mass ratio of 1:3, the growth of molds such as Aspergillus versicolor and Penicillium purpurescens and bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus can be inhibited, and the effect is synergistic. Compared with the use of p-hydroxyacetophenone alone, the inhibitory effect on various microorganisms can be improved, the resistance of the water-light needle skin care composition to external microorganisms can be improved, and the storage time of the water-light needle skin care composition can be prolonged. At the same time, the addition amount of the preservative component in the water-light needle skin care composition can be reduced, and the influence on human skin and health can be avoided.

This invention relates to the field of bioengineering technology, specifically to an extracellular vesicle from kelp, its preparation method, and its applications. This invention provides a method for efficiently isolating extracellular vesicles with specific biological activities from kelp, and an application scheme for these vesicles to promote the proliferation, migration, and maintenance of stemness of large yellow croaker muscle stem cells under low serum conditions. This overcomes the problems of high cost and limited functionality of fetal bovine serum as a substitute in existing cell-cultured meat technologies, providing a novel culture medium supplement component that is stable in origin, low in cost, and has a clear effect.

The invention discloses an extraction method of laminarin. The method comprises the following steps: 1) pretreating wet kelp by adopting steam explosion, drying and crushing to obtain powder; 2) carrying out first extraction on the powder by adopting a first organic solvent, and carrying out solid-liquid separation to obtain a first precipitate; (3) carrying out secondary extraction on the first precipitate and an inorganic solvent, and carrying out solid-liquid separation to obtain supernate; 4, the supernate and a second organic solvent are subjected to third extraction and solid-liquid separation, a second precipitate is obtained, and the laminarin is obtained.By means of the extraction method, the extraction rate of the laminarin can be increased, the molecular weight of the laminarin can be reduced, and the uronic acid content can be increased.

The application belongs to the technical field of fishery culture, and provides a low fish meal compound feed with low oxygen tolerance, a preparation method and application thereof, the low fish meal compound feed contains the following raw materials: fish meal, soybean meal, soybean protein isolate, flour, chicken meal, cassava starch, fish oil, soybean oil, lecithin, calcium dihydrogen phosphate, compound vitamin, compound mineral substance, choline, vitamin C, DL-Met, Lys-HCL, Thr, astaxanthin and chrysoflagellate laminarin polysaccharide. Firstly, the compound plant protein such as soybean meal and soybean protein isolate and chicken meal are used to replace the traditional fish meal in the application, the amino acid composition is balanced, the nutritional proportion of the compound feed is optimized, and the proportion of fish meal is reduced to 20%; secondly, the compound antioxidant such as astaxanthin and chrysoflagellate laminarin polysaccharide is added in the compound feed, and the fish body antioxidant capacity can be significantly improved.

The invention discloses a glucanase SDuLam55A gene, a recombinant glucan rSDuLam55A enzyme, a method and an application of the recombinant glucan rSDuLam55A enzyme. The nucleotide sequence of the StuLam55A gene is as shown in SEQ ID NO: 1, the StuLam55A gene is derived from Stropharia rugoso-annulata, and the coded recombinant glucan rStuLam55A enzyme (96kDa) has strict substrate specificity and only aims at hydrolysis of beta-1, 3-glucosidic bonds in laminarin. The invention provides a method for cloning the gene from stropharia rugoso-annulata hyphae and a process for efficiently preparing the rSDuLam55A enzyme through a recombinant expression system. The enzyme has remarkable application value in the fields of penicillium growth inhibition, agricultural waste treatment, food preservation and the like, and high stability, strict substrate specificity and efficient catalytic characteristics of the enzyme provide a safe and environment-friendly novel enzyme preparation solution for related industries.

The present application provides a kind of laminarin-western radix astragali glue-silk microfiber medical catheter and its preparation method, and the medical catheter preparation solution contains the following components per 100ml: laminarin 1-10g, aqueous solution 10-100mL, 10% radix astragali glue solution 2-30mL, silk microfiber 1-10g.The material of the present application is low in price, and the preparation method is simple and easy to operate, which avoids the cytotoxicity side effects caused by the use of toxic crosslinking agent, has good biocompatibility, and at the same time, the prepared biomimetic gel catheter has good porosity, mechanical strength and good flexibility, is suitable for blood vessels, peripheral nerve catheter and other applications, and is easy to metabolize in vivo.

The present invention relates to a composition comprising a water-soluble active substance, such as laminarin. In particular, the present invention relates to a stable composition comprising a water-soluble active substance, such as laminarin, and a polyol. The present invention also relates to a process for preparing said composition and methods of use thereof.

This invention discloses a kelp fucoidan, its preparation method, and its application. The kelp fucoidan includes LF3-1 and / or LF4-1. The main chain of LF3-1 is composed of alternating →3)-α-L-Fucp-(1→ and →3)-β-D-Galp-(1→). The fucose residues on the main chain of LF3-1 are sulfated at the C-4 position or connected to a first branch, which is composed of →4)-α-L-Fucp-(1→. The galactose residues on the main chain of LF3-1 are sulfated at the C-6 position or connected to a second branch, which is composed of →2,3)-β-D-Manp-(1→ and β-D-Galp-(1→). The sulfate content of LF3-1 is 26.3%, and the weight-average molecular weight of LF3-1 is 305. LF4-1 has a molecular weight of 32.7% and a weight-average molecular weight of 182.1 kDa. Its main chain consists of alternating links of →3)-α-L-Fucp-(1→ and →6)-β-D-Galp-(1→). The fucose in the main chain of LF4-1 is sulfated at C-4 or has a third branch, which is composed of →4)-α-L-Fucp-(1→. The galactose in the main chain of LF4-1 is sulfated at C-3 or has a fourth branch, which is composed of →2,4)-β-D-Manp-(1→ and β-D-Galp-(1→). LF4-1 effectively improves blood lipids and insulin resistance, promotes lipid oxidation and glucose metabolism gene expression, and thus plays a role in weight loss and lipid reduction.

According to the laminaria proteoglycan purified extract and the preparation method and application thereof, in the preparation process of the purified extract, an ultrasonic-assisted water extraction method and anion exchange chromatography are adopted for purification, the extraction efficiency is high, the purification effect is good, laminaria proteoglycan effective ingredients in laminaria can be effectively enriched, and the content of laminaria proteoglycan in laminaria is increased. The obtained extract is high in purity and high in activity, high-value utilization of kelp resources is achieved, kelp proteoglycan is used as a whole for extraction, purification and activity research, and the synergistic effect of kelp proteoglycan is fully exerted. In the invention, a caenorhabditis elegans model, a zebra fish model and a human renal epithelial cell (HK-2) model verify that the laminarin purified extract has a remarkable protective effect on the body injury caused by the microplastics, and a new candidate medicine and a scientific basis are provided for developing the microplastic injury protection medicine.

The application belongs to the technical field of cosmetics, and discloses a fucoidan composition, a preparation method and application thereof. The fucoidan composition comprises adiantum capillus-veneris fucoidan, ralfsia dactylifera fucoidan and pseudochoricystis sp. fucoidan, and the mass ratio of the adiantum capillus-veneris fucoidan, the ralfsia dactylifera fucoidan and the pseudochoricystis sp. fucoidan is (1-2):(1-2):(1-2). The fucoidan composition is prepared by compounding specific adiantum capillus-veneris fucoidan, ralfsia dactylifera fucoidan and pseudochoricystis sp. fucoidan, and has excellent whitening, anti-wrinkle, moisturizing and antioxidant multiple effects through synergistic effect. When the fucoidan composition is applied to cosmetics, it has good formula adaptability, does not affect the exertion of the effects and the stability of the cosmetics.

The invention provides an incision beta-1, 3-glucanase and application thereof, and the amino acid sequence of the incision beta-1, 3-glucanase is SEQ ID NO: 1. The endo-beta-1, 3-glucanase disclosed by the invention can efficiently hydrolyze laminarin and gellan, the enzyme activity of the endo-beta-1, 3-glucanase can still be kept at 80% or above within the temperature range of 40-60 DEG C and the pH value range of 4.5-6.0, and the endo-beta-1, 3-glucanase shows relatively good stability under medium and low temperature conditions, and is beneficial to reducing energy consumption in a practical application process. Meanwhile, the enzyme has remarkable high salt resistance and has certain tolerance to ethanol with the concentration of 5%-15%. The endo-beta-1, 3-glucanase disclosed by the invention has an obvious inhibition effect on formation of a candida albicans biological membrane, and kelp oligosaccharide generated by hydrolyzing laminarin also shows good antioxidant capacity, so that the endo-beta-1, 3-glucanase has a wide application prospect and a remarkable practical value in the fields of antibiosis, biological medicine and the like.

The objective is to provide therapeutic drugs that promote wound healing. [Solution] Analysis of Dectin-1 gene-deficient mice revealed that inhibiting Dectin-1 promotes wound healing caused by burns. When laminarin, a Dectin-1 antagonist, or barley β-glucan 30sp was administered to the wound, a significant wound healing promoting effect was observed. Since low molecular weight β-glucan, a Dectin-1 antagonist, has the effect of promoting wound healing, a wound healing promoter containing it as an active ingredient can be provided.

The invention discloses ruminant feed based on aged corn and laminarin and application of the ruminant feed, and belongs to the technical field of animal feed. The aged corn is used for replacing common corn to serve as a main energy raw material, 0.5% of laminarin is added, and the problems of growth inhibition, oxidative stress, rumen microecological imbalance and the like caused by the aged corn are solved. Tests show that the feed can significantly improve the average daily gain, the nutrition digestibility and the antioxidant capacity of the Hu sheep, improve the meat quality and reduce the breeding cost, and a new way is provided for reasonable utilization of the aged corn.

This invention relates to the field of functional pet food technology, specifically to a freeze-dried product of fucoidan-alginic acid oligosaccharide bicolor crystal spheres with an inner and outer partition structure and its preparation method. This invention achieves spatial distribution of different active ingredients by constructing a partition structure with a core region and an outer shell region, thereby ensuring structural stability while meeting the functional application requirements in pet food systems. The freeze-dried product is a crystal sphere particle with an inner and outer partition structure, comprising: a core region containing fucoidan and protein active ingredients; and an outer shell region covering the outside of the core region and containing alginate oligosaccharide; wherein: the crystal sphere particles are freeze-dried to form a porous structure; and the core region and outer shell region form a spatial partition structure.

The application discloses a special fertilizer for vegetables, a preparation method and application thereof, and belongs to the field of fertilizer technology.The special fertilizer for vegetables comprises functional bacteria, bacteriostatic and disease-resistant agents and mixed mineral particle carriers; the functional bacteria and the bacteriostatic and disease-resistant agents are adsorbed in the mixed mineral particle carriers; the functional bacteria comprise high-efficiency nitrogen-fixing bacteria, phosphorus-dissolving bacteria and potassium-dissolving bacteria; and the bacteriostatic and disease-resistant agents are laminarin and chlorogenic acid.The special fertilizer for vegetables has the effects of nitrogen fixation, phosphorus dissolution and potassium dissolution, can meet the demand of vegetables for nitrogen, phosphorus and potassium nutrients in a whole growth season, has long-lasting fertilizer efficiency, balanced nutrient supply, can improve soil, overcome continuous cropping obstacles, enhance plant disease resistance, is environment-friendly, can improve the yield and quality of vegetables, and realizes sustainable production of high-quality vegetables.

The application provides a laminarin proteoglycan purified extract and a preparation method and application thereof. In the preparation process of the purified extract, ultrasonic-assisted water extraction and anion exchange chromatography are used for purification, the extraction efficiency is high, the purification effect is good, the effective components of laminarin proteoglycan in laminaria can be effectively enriched, the obtained extract has high purity and strong activity, the high-value utilization of laminaria resources is realized, the laminarin proteoglycan is extracted and purified as a whole and activity research is conducted, and the synergistic effect is fully exerted. In the application, through the Caenorhabditis elegans model, the zebra fish model and the human kidney epithelial cell (HK-2) model, it is verified that the laminarin proteoglycan purified extract has a significant protection effect on the damage of the organism caused by microplastics, and a new candidate drug and scientific basis are provided for the development of a microplastic damage protection drug.