147 results about "Laminarin" patented technology

The invention relates to an optionally foamed sealing composition for preformed seals which can swell when treated with water. The invention also relates to a method for the production thereof out of natural rubber and/or elastomers with a matrix comprised of natural rubber/elastomer components and particle-shaped water absorbing material stored therein. The water absorbing material is a combination of (A) polysaccharide(s) selected from cellulose, starch, starch derivatives removed from grafted starch, amylose, amylopectin, dextran, pectin, inulin, chitin, xanthan, alginic acid, alginates, carrageenan, pustulan, callose, laminarin, guluronic acid, pullulan, lichenin or mixtures of the same with (B) a highly water absorbent synthetic polymer selected from polymers based on (meth)acrylate, poly(meth)acrylic acid and the salts thereof, polyacrylamide, polyalcohols or copolymers of said synthetic polymers. The invention also relates to additional cross-linking and processing auxiliary agents and to property improving agents. It is possible to securely seal superstructures, substructures, tunnels and canals with the assistance of the inventive sealing compositions.

The invention discloses a method for extracting laminarin from kelp. The method comprises the following steps: raw material treatment, ultrasonic treatment, enzymolysis, destaining, deproteinization and drying to obtain the product. Compared with the existing trichloroacetic acid precipitation method, savage method and the like, the method has the advantages that laminarin is high in yield and high in content.

The invention discloses a biological cell freezing antifreeze, and 100ml of the cell freezing antifreeze consists of the following raw materials: 0.025 to 0.3g of kelp polysaccharide, 2.0 to 3.5g of glucose, 0.5 to 1.5g of sodium citrate, 60 to 80ml of distilled water, 10 to 20ml of albumin, 5 to 18ml of glycerol and 0.05 to 0.15 million units of penicillin. The preparation method is that: the glucose and the sodium citrate are dissolved in the distilled water to prepare a base liquid; the albumin is added, the mixture is mixed evenly, and the penicillin is added to prepare a I liquid; the glycerol is added in the I liquid to prepare a II liquid; the kelp polysaccharide is added in the II liquid for even mixing; the pH value is adjusted to 6 to 7.5, then the filtration and sterilization are carried out, and the mixture is cooled until reaching the room temperature and then arranged in a refrigerator of 2 to 5 DEG C for standby. The cell freezing antifreeze is applicable to the cryopreservation of mammalian semen, somatic cells, oocytes, early embryos, organs and tissues, in particular to the cryopreservation of the semen of various mammals.

The invention relates to the fields of medicine and biology, in particular to an organ preservation solution and a preparation method thereof. Every 1000mL of the organ preservation solution is prepared from the following components by weight: 3.0-15.0g of sodium citrate, 10.0-30.0g of potassium citrate, 5.0-15.0g of mannitol, 0.5-1.2g of potassium chloride, 0.5-1.8g of magnesium sulfate heptahydrate, 0.5-25.0g of a pH buffer pair, 0.2-25.0g of energy substrate and the balance of water for injection. The organ preservation solution also contains one or more of laminarin, trehalose, laminine and betaine. The organ preservation solution is low in price and efficient, and is suitable for preserving multiple organs.

The invention discloses a laminaria japonica polysaccharide, which is prepared by a method in the steps of: adding water into crushed laminaria japonica, adjusting the pH value to 1-4, conducting extraction for 2-5h at a temperature of 100-120DEG C; carrying out filtration so as to obtain an extracting solution, the pH value of which is adjusted to 6.3-7.0 by potassium-containing alkali, concentrating the extracting solution to a concentrated solution with solid content of 35-48%; adding ethanol into the concentrated solution and mixing them well, then leaving the solution to stand at a temperature of 4DEG C for 4-12h, and performing filtration so as to obtain a polysaccharide precipitate; implementing freeze drying, thus obtaining the laminaria japonica polysaccharide. The laminaria japonica polysaccharide, accounting for 0.01-1.0% of cut tobacco by mass, can be prepared into a solution. And the laminaria japonica polysaccharide solution can be added into cut tobacco or a filter tip through sprinkling so as to obviously enhance cigarette smoking quality, and reduce stimulation on a nasal cavity and throat. Accordingly, the laminaria japonica polysaccharide enables fine and smoothsmoke, soft mouthfeel, maintained aroma, and no side effect. The laminaria japonica polysaccharide provided in the invention has the advantages of no fishy smell, high extraction rate, easy acquisition, low cost and good oxidation resistance.

The invention provides complex immunoenhancer for prawns. The complex immunoenhancer is prepared from, by weight, 5-10% of laminarin, 10-20% of astragalus polysaccharide, 20-30% of zymosan, 20-30% of chitosan, 5-10% of garlicin, 5-15% of vitamin C and 10-30% of wheat middling. The raw materials are ground through an ultrafine grinder, screened by a 100-mesh screen and then evenly mixed quantitatively according to the ratio, and the adding proportion of the complex immunoenhancer in fodder is 0.2-0.5%. The complex immunoenhancer can obviously enhance the immunity of the bred prawns, can promote the growth of the prawns and is free of toxins, harm and residues.

The invention discloses a processing method of a fish ball with high elasticity and enriched in laminarin and belongs to the field of food processing. According to the method, marine fishes are taken as raw materials, and micronized kelp homogenate is added in a targeted way, so that the absorbtion and utilization rate of the laminarin is improved, and the nutritional value of the fish ball is enhanced; by comprehensively applying a low-temperature steam and microwave water bath combined two-stage type step-by-step gel process, a fish ball product with high elasticity and enriched in laminarin is developed. The processing method disclosed by the invention comprises the following steps of preparing the micronized kelp homogenate; selecting materials, separating flesh, pretreating, rinsing, and performing fine filtration classification and dehydration; kneading and forming; performing two-stage type step-by-step gelling, cooling and freezing. The fish ball disclosed by the invention is delicious in taste, novel in color and rich in mineral trace elements and the laminarin essential for a human body, and is a novel fish ball with higher nutritional value and better gelling property.

The invention provides a kelp degrading strain having kelp degrading activity. The strain is conserved in China General Microbiological Culture Collection Center, is classified and named as Tamlana alginolytica, and has the conservation number of CGMCC No. 5324. The provided strain is capable of generating an extracellular compound enzyme system having the activity of degrading various polysaccharides and also capable of realizing deep degradation of kelp. The invention provides two kelp juice preparation processes without adding an extracting medium and without heating and heat preservation assistance, namely a bacterial treatment process with kelp pieces as the raw material and an enzymatic treatment process with kelp powder as the raw material; the two processes both are capable of degrading kelp polysaccharides into reducing oligosaccharides or monosaccharides through biodegradation under mild conditions, and simultaneously achieving the effect of deodorization. According to the invention, the comprehensive utilization rate and eating quality of the raw material are improved and technical support is provided for refined and deep processing of kelp; as a result, the kelp degrading strain has enormous application prospect in the development aspect of kelp beverage.

The invention discloses a composite biological fresh-keeping agent used for squid, and a fresh-keeping method thereof. The composite biological fresh-keeping agent comprise a squid fresh-keeping agent 1 and a squid fresh-keeping agent 2; the squid fresh-keeping agent 1 and the squid fresh-keeping agent 2 are combined for fresh keeping of squid; the squid fresh-keeping agent 1 is prepared from enzymolysis products of flounder leftovers; the squid fresh-keeping agent 2 is prepared from laminarin, pine bark polyphenol extract, and resveratrol. The fresh-keeping method comprises following steps: fresh squid is washed; vesicle is removed; squid is cut into pieces, is uniformly immersed into the squid fresh-keeping agent 1 and the squid fresh-keeping agent 2 respectively, is taken out from the squid fresh-keeping agents, and is subjected to natural air drying at 4 DEG C; the obtained dried squid is stored in fresh-keeping bags; and the fresh-keeping bags are sealed, and are stored at 0 DEG C. The composite biological fresh-keeping agent also possesses antibacterial effect and oxidation resistance, and is capable of reducing formaldehyde content in squid, and prolonging self life of squid; synergistic effect of the composite biological fresh-keeping agent in maintaining of the quality of squid is achieved; and no obvious influence on the color of squid is caused.

The invention discloses novel application of laminaria japonica/laminarin extracts. The novel application includes application of the laminaria japonica/laminarin extracts to preparing EV71 resistant medicine. A method for preparing the laminarin extracts can be one of a method (1), a method (2) and a method (3). The method (1) for preparing the extracts from laminaria japonica water includes washing laminaria japonica by the aid of PBS (poly butylenes succinate); boiling the laminaria japonica in double-distilled water; filtering the double-distilled water to obtain boiled juice; centrifuging the boiled juice to obtain supernatant so as to obtain the extracts. The method (2) for preparing aqueous extraction ethanol sediments from laminaria japonica includes washing the laminaria japonica by the aid of PBS; boiling the laminaria japonica in double-distilled water; filtering the double-distilled water to obtain boiled juice; adding anhydrous ethanol into the boiled juice until the concentration of the ethanol reaches 75-95% to obtain mixed liquid; centrifuging the mixed liquid to obtain the aqueous extraction ethanol sediments so as to obtained the extracts. The method (3) for preparing separation components of laminaria japonica by the aid of DEAE (diethyl-aminoethanol) cellulose columns includes separating the aqueous extraction ethanol sediments from one another by the aid of the DEAE cellulose columns; eluting the aqueous extraction ethanol sediments by the aid of NaCl solution; collecting eluted components to obtain the extracts. The novel application has the advantages that experimental bases are provided for clinically treating EV71 infectious diseases by the aid of the laminaria japonica extracts, and the application has a certain guidance significance on developing the EV71 resistant medicine and has important reference value.

The invention relates to a bactericide composition containing fluoride ether bacteria amide and laminarin. The bactericide composition comprises a suspending agent, wettable powder, water dispersible granules and the like prepared from the fluoride ether bacteria amide, the laminarin and auxiliary agents including a wetting dispersant, a filling agent and the like. An experiment research shows that the fluoride ether bacteria amide and the laminarin are mixed according to a ratio from (10:1) to (50:1); a systemic fungicide is combined with a disease-resistant inducing agent so that the bactericide composition has an obvious synergistic effect on cucumber downy mildew, and can also delay a disease progress; the dosage of the chemical pesticide fluoride ether bacteria amide is reduced and the resistance to the fluoride ether bacteria amide by pathogenic bacteria can be effectively delayed. The bactericide composition is suitable for preventing and treating many vegetable oomycete diseases including the cucumber downy mildew, tomato phytophthora diseases, phytophthora capsici, potato late blight and the like, and can also be used for preventing and treating cotton seedling stage diseases including cotton red rot diseases and the like.

The invention discloses a bio-based hemodialysis membrane and a preparation method thereof. The hemodialysis membrane is prepared from polylactic acid, laminarin, thiolated chitosan, silk fibroin, sodium ascorbyl phosphate, cellulose acetate, dithiothreitol, stachyose, betulin caffeate, ammonium persulfate, ferulic acid, hydroxyethyl starch, N-benzyl glycine hydrochloride, deoxycholic acid, polyhydroxyalkanoate, hexamethylenediamine, hyaluronic acid, benzoperoxide and dimethyl sulfoxide. According to the prepared hemodialysis membrane, the tensile strength is not lower than 7.0 MPa, the transmittance of urea is higher than 86%, the transmittance of beta2 microglobulin is higher than 66%, the rejection rate of macromolecular protein is higher than 95%, the biocompatibility is good, coagulation, thrombus, inflammation, poisoning and other adverse reactions do not exist, and an application prospect is wide.

The invention discloses a preparation method and an application of an ethanol extract of laminarin. The ethanol extract of laminarin is obtained through the preparation method which comprises the following steps: 1, crushing sea tangle, adding an ethanol solution to the crushed sea tangle, extracting for 2-5h at 50-80DEG C; 2, filtering to remove a residue, and adding active carbon to an extract to decolor and remove a fishy smell; and 3, filtering, and concentrating to obtain the ethanol extract of laminarin. The ethanol extract of laminarin is added to cut tobaccos or filter tips in a sprinkling mode, and the mass of the ethanol extract of laminarin accounts for 0.01-1.0% of the mass of cut tobaccos, so the content of free radicals in a mainstream smoke of cigarettes is effectively reduced, the reduction amplitude of the content of the free radicals is more than 15%, and the smoking quality of the cigarettes is improved, thereby the ethanol extract of laminarin, which has irritationreduction effects on nasal cavities, throats and the like, allows the smoke to be smooth, the taste to be soft, the fragrance to be kept, and no side effects to be possessed. The ethanol extract of laminarin has the advantages of no obviously fishy smell, easy obtaining, and low cost.

The invention provides a method for dealuminizing salted jellyfishes. The method comprises the following steps: dissolving blueberry leaf flavonoids into de-ionized water and agitating uniformly; adding calcium chloride, acetic acid and laminarin solutions to prepare a jellyfish dealuminizing fresh-keeping solution containing the following components in mass percent: 0.05%-0.5% blueberry leaf flavonoids, 0.05%-0.5% calcium chloride, 0.01%-0.2% acetic acid and 0.1%-0.5% laminarin; washing the salted jellyfishes and immersing the jellyfishes into the jellyfish dealuminizing fresh-keeping solution; and draining off and carrying out vacuum packaging by using a sterile fresh-keeping bag. The method has the advantages of simplicity and convenience in operation and low cost; aluminum in the jellyfishes can be removed effectively, the original crispness of the jellyfishes is kept, the mouthfeel is good and a dealuminizing effect is good; an antibacterial effect is good and the shelf life of jellyfish products can be prolonged; the blueberry leaf flavonoids and the laminarin are added so that the salted jellyfishes are nutritional and healthy and the safety of foods is guaranteed.

The invention discloses a degradable biological starch film capable of being repeatedly used. The degradable biological starch film is prepared from the following raw materials in parts by weight: 64 to 70 parts of dry basis corn starch, 23 to 26 parts of ethyl alcohol, 11 to 15 parts of monochloroacetic acid, 14 to 16 parts of silicon dioxide, 0.08 to 0.1 part of epoxy chloropropane, 60 to 80 parts of dimethylformamide, 3 to 4 parts of laminarin, 2 to 4 parts of glycerin, 4 to 6 parts of tributyl citrate, 0.2 to 0.3 part of antioxidants 1010, 6 to 8 parts of polyving akohol, a proper amount of 85-percent ethanol solution and a proper amount of deionized water. The prepared starch film has the full degradation performance; the environment pollution caused by conventional plastics can be avoided; no hard is caused on soil; green and environment-friendly effects are achieved; the preparation process is simple; the cost is low; the degradable biological starch film is worthy of being popularized.

The invention discloses an anti-radiation beverage, which is made of the following main components: laminarin, Lycium barbarum polysaccharide, tea polyphenol, citric acid, sucrose, vitamin C and the like. The beverage has an anti-radiation efficacy and does not contain preservative and pigment, the proportioning is reasonable and scientific, the beverage is crystal clear and is in light beer color, the taste is fresh and cool, the sour and sweet tastes are moderate and the healthcare effect is remarkable.

The invention discloses a method for extracting laminarin with anti-tumor activity from kelp. The method comprises the following steps: raw material treatment, ultrahigh pressure treatment, ultrasonic treatment, alginate removal, decoloration, protein removal, and drying, so as to obtain the product. An experiment proves that the obtained laminarin has good anti-tumor activity.

The invention discloses a method for extracting and separating laminarin with low molecular weight and application thereof in cigarette products. The laminarin with the low molecular weight organic acid is extracted by adopting an organic acid method. After kelps are smashed, the laminarin with the low molecular weight organic acid is obtained through organic acid extraction, ultra-filtration, concentration and ethanol fractional precipitation and separation, the molecular weight of the laminarin is less than 10000 Dalton, and solid powder is light brown. The method for extracting and separating the laminarin with the low molecular weight is simple and suitable for industrialization production, the obtained laminarin with the low molecular weight serves as a cigarette ingredient to be applied to cigarettes, smoking quality is improved remarkably, miscellaneous gases are removed effectively, fragrance is improved and embellished to be fine and smooth, smoke is soft, smoke quality is improved obviously, stimulation to nasal cavity, throat and the like is reduced, and the laminarin has positive effect.

The invention belongs to the technical field of polysaccharide derivatives, and particularly relates to a preparation method of modified laminarin by a synergistic effect. A low-thermosetting phase one-step synthesis method with advantages of low reaction temperature, low energy consumption and simple operation steps is adopted and organic solvents are not used or less used, the environmental pollution can be reduced, so that the requirements of green chemistry are met, and the method has the potential of development and utilization. 3-amino-1-hydroxypropylenedi modification is carried out onlaminarin, phosphonyl group, amidogen and hydroxyl can be introduced, a hydrophilic surface structure can be constructed for the laminarin, so that the water solubility activity of the laminarin can be effectively improved. Compared with the prior art, the phosphate radical content and calcium content of the prepared 3-amino-1-hydroxypropylenedi laminarin product are both greatly improved, the laminarin is green and environmentally friendly, the operation process is simple and operated easily, and the industrialization and technical promotion are convenient.

The invention provides laminarin and an extraction and separation method thereof. The method comprises the following steps: extracting a laminarin crude extract having a yield of 3.6 percent, which is superior to that of a traditional water extraction method, by adopting a high-temperature high-pressure water extraction method; performing two-step separation by DEAE-anion exchange chromatography and Sephacryl S-300 gel chromatography to obtain a kelp purified polysaccharide PS-3-1 having a molecular weight of 132 KDa. The prepared kelp purified polysaccharide has free radical scavenging activity, has a scavenging rate for hydroxyl radicals and superoxide anion being over 70 percent, and can be developed into a free radical scavenging drug. Furthermore, the purified polysaccharide has a reproduction inhibiting activity on vascular smooth muscle cells, has a reproduction inhibition rate of over 75 percent, and can be used for preparing a vascular smooth muscle cell reproduction inhibitor medicine.

The invention discloses laminarinase OUC-L1. The amino acid sequence of the laminarinase OUC-L1 is shown as SEQ ID NO:1, the gene of the laminarinase OUC-L1 is encoded, and the nucleotide sequence ofthe laminarinase OUC-L1 is shown as SEQ ID NO. 2. The laminarinase OUC-L1 is explored from a human intestinal bacterium A. muciniphila genome, belongs to a glycoside hydrolase GH16 family, and can effectively hydrolyze laminarin, and the main product of the laminarinase OUC-L1 is three parts of oligosaccharides, has high specific enzyme activity and good biological catalysis efficiency, and has the highest reaction activity at the temperature of 35 DEG C. In addition, the laminarinase OUC-L1 also has good cold adaptability. The invention further discloses an enzyme preparation containing the laminarinase OUC-L1. The enzyme preparation still has considerable catalytic activity at low temperature and has good industrial application potential.

A granular laminarin for the patients of diabetes and obesity is prepared from fresh seaweed through extracting in boiling water, adsorbing by macroreticular resin, eluting, adsorbing by polyamide column, eluting, spray drying, proportionally mixing it with xylitol, non-sugar filler and adhesive, granulating, drying and sterilizing.