38 results about "Laminarin" patented technology

A preparation method of a bacteriostatic and antioxidant zein / hyaluronic acid degradable preservative film relates to the field of foods.The method comprises the steps that a zein-hyaluronic acid solution and a citrus polyphenol solution are mixed, distilled water is dropwise added, vacuum concentration is performed, the mixture is mixed with a laminarin solution, auxiliary materials are added, uniform mixing, bubble removing and film preparing are performed, and the preservative film is obtained. After drying, obtaining a zein-HA film of laminarin and citrus polyphenol; or mixing the zein-hyaluronic acid solution with the moringa oleifera leaf extract powder, dropwise adding distilled water, concentrating under reduced pressure, mixing with the laminarin solution, adding the sodium alginate solution and auxiliary materials, defoaming, pouring to form a film, and drying to obtain the zein-HA film of the laminarin and the moringa oleifera leaf extract. The synergistic effect among the substances of the protein film can effectively improve the fresh-keeping effect of the composite film, and the release property of the composite film can further improve the fresh-keeping effect on fresh-cut food.

The invention discloses a method for simultaneously preparing low-molecular-weight algin and fucoidin, and belongs to the field of food processing. The multi-dimensional swing type high-energy nano impact mill is adopted, through rapid multi-dimensional swing type movement of the tank body, irregular movement is increased, huge impact force is generated, the movement track of a grinding medium is prolonged, impact energy is improved, impact blind spots are reduced, and the crushing speed is greatly increased; and preparing algin and fucoidin with low molecular weight. Through a physical crushing technology, the fucoidin does not need to be treated after algin and polysaccharide are extracted and separated, pre-freezing is not needed, and the undaria pinnatifida sporophyll is crushed and extracted by using a multi-dimensional swing type high-energy nano impact mill, so that the method is environment-friendly, safe and efficient; and compared with known ultrasonic and infrared technologies, the method has the advantages of higher yield, simple process, wide application range and the like, and is suitable for preparing low-molecular-weight algin and fucoidin from various sources.

The invention discloses an esophageal cancer targeting nano-tablet based on a traditional Chinese medicine composition and a preparation method of the esophageal cancer targeting nano-tablet. The targeted nano tablet is prepared from the following refined extracts: a rhizoma paridis total saponin extract, a pseudobulbus cremastrae seu pleiones colchicine derivative extract, a panax notoginseng total saponin extract, a carthamin yellow extract, a thunberg fritillary bulb total alkaloid extract, frankincense-myrrh compound volatile oil, a resin extract and an astragaloside IV extract. The nano-carrier comprises a nano-carrier, a seaweed-laminarin composite extract, a processed rhizoma pinelliae total alkaloid extract and costus root volatile oil, and the costus root volatile oil serves as one of targeting ligands to be used for modifying the nano-carrier. The targeting property is strong: the dual-targeting ligand (costunolide-TOPK affinity peptide) on the surface of the nano-carrier can actively recognize and combine with a specific receptor of esophageal cancer cells, and meanwhile, due to the size (about 100-200 nanometers) of the carrier, the carrier is easy to enrich in tumor tissues through enhanced permeation and retention (EPR) effect, so that the modernization upgrading of the idea of'channel guiding medicine 'is realized.

The application belongs to the technical field of cosmetics, and discloses a fucoidan polysaccharide composition with bacteriostatic efficacy, and a preparation method and application thereof. The fucoidan polysaccharide composition comprises phaeophycus triquetrum fucoidan, small new moon rhombus fucoidan and false micro-halochain fucoidan, and the mass ratio of the phaeophycus triquetrum fucoidan, the small new moon rhombus fucoidan and the false micro-halochain fucoidan is (1-2):(1-2):(1-2). The fucoidan polysaccharide composition is prepared by compounding specific phaeophycus triquetrum fucoidan, small new moon rhombus fucoidan and false micro-halochain fucoidan, has a synergistic effect, and has excellent antibacterial efficacy. When the fucoidan polysaccharide composition is applied to cosmetics, it has good formula adaptability, does not affect the exertion of efficacy and the stability of the cosmetics.

The invention discloses a preparation method and application of ulcerative colitis resisting kelp fucoidin, and belongs to the technical field of polysaccharide preparation and application, the preparation method is used for preparing the kelp fucoidin, and the preparation method comprises the following steps: taking kelp as a raw material, cleaning, drying, crushing and sieving, and then performing reflux degreasing to obtain kelp powder; carrying out hot water-ultrasonic synergistic extraction on the algae powder, and carrying out centrifugation and rotary evaporation concentration to obtain a supernatant; performing graded alcoholization and deproteinization treatment on the supernate to obtain a deproteinized polysaccharide solution; and dialyzing the deproteinized polysaccharide solution to remove impurities, freeze-drying to obtain the kelp fucoidin, and applying the obtained kelp fucoidin to treatment of ulcerative colitis. The preparation process is simple, efficient and high in repeatability, compared with an artificially synthesized ulcerative colitis resisting medicine mesalazine, the obtained natural fucoidin has the same level of ulcerative colitis resisting activity, a new natural active raw material is provided for ulcerative colitis treatment, and the natural fucoidin has a good application prospect.

The invention discloses laminarin yoghourt and a preparation method thereof, and belongs to the technical field of food processing. The preparation method specifically comprises the following steps: (1) mixing raw milk, white granulated sugar, a water-extracted laminarin aqueous solution and water, sterilizing and cooling; (2) adding a leavening agent, fermenting and demulsifying; and (3) stirring, cooling, filling, carrying out low-temperature after-ripening, and carrying out cold storage, thereby obtaining the product. The laminarin is added into the yoghourt, so that the viable count of lactic acid bacteria in the shelf life can be remarkably increased, the taste is improved, and the laminarin yoghourt is widely applicable to stirring type yoghourt, drinking type yoghourt, solidification type yoghourt, browning yoghourt and the like.

The invention belongs to the technical field of fishery breeding, and provides a low-oxygen-resistant low-fish-meal compound feed as well as a preparation method and application thereof. The low-fish-meal compound feed is prepared from the following raw materials: fish meal, soybean meal, soybean protein isolate, flour, chicken meal, cassava starch, fish oil, soybean oil, lecithin, monocalcium phosphate, compound vitamins, compound minerals, choline, vitamin C, DL-Met, Lys-HCL, Thr, astaxanthin and chrysophyta laminarin. Firstly, the soybean meal, the soybean protein isolate and other composite plant proteins and the chicken meal are adopted to replace traditional fish meal, amino acid composition is balanced, the nutrition proportion of the compound feed is optimized, and the proportion of the fish meal is reduced to 20%; in addition, compound antioxidants such as astaxanthin and chrysophyta laminarin are added into the compound feed disclosed by the invention, so that the oxidation resistance of fish bodies can be remarkably improved.

The application discloses sodium alginate-kelp polysaccharide-ganoderic acid D nanoparticles for targeted treatment of ulcerative colitis and a preparation method and application thereof. Raw materials of the nanoparticles include sodium alginate, kelp polysaccharide and ganoderic acid D with a mass ratio of 20-30:2-5:8-12. The sodium alginate-kelp polysaccharide-ganoderic acid D nanoparticles are prepared by taking sodium alginate gel as a carrier and loading kelp polysaccharide and ganoderic acid D in the carrier, and the nanoparticles are used for preventing and / or relieving ulcerative colitis, can have antioxidant effect, target regulation of intestinal barrier function, reduction of inflammatory reaction and improvement of intestinal health, and provide a new method for ulcerative colitis.

The invention discloses a method for producing chrysophyceae laminarin through high-density culture of chrysophyceae and an application of the chrysophyceae laminarin. The culture method comprises the following steps: (1) culturing a chrysophyceae species under low-temperature and low-light conditions, and then transferring the chrysophyceae species into a seed culture medium for culturing to obtain activated chrysophyceae species; (2) placing the activated algae species obtained in the step (1) under a dark condition for shake culture to obtain dark adaptation algae species; and (3) inoculating the dark adaptation algae seeds into a fermentation tank, and adding a basic culture medium and a fed-batch culture medium for fermentation culture. By selecting the nitrogen source and accurately controlling the ammonium concentration, the double improvement of the biomass and the yield of the chrysophyceae laminarin is realized, the adverse effect of the high-concentration nitrogen source on growth is avoided, and the culture efficiency and the product economy are improved.

The present application provides a kind of endo beta-1, 3-glucanase and its application, the amino acid sequence of the endo beta-1, 3-glucanase is SEQ ID NO:1.The endo beta-1, 3-glucanase of the present application can efficiently hydrolyze laminarin and curdlan, its enzyme activity can still be kept above 80% in the range of 40-60 DEG C and pH 4.5-6.0, and it shows good stability under low temperature conditions, which is conducive to reduce the energy consumption in the process of practical application.At the same time, the enzyme has significant high salt tolerance, and has certain resistance to 5%-15% ethanol.The endo beta-1, 3-glucanase of the present application has obvious inhibitory effect on the formation of Candida albicans biofilm, and the laminarin oligosaccharide generated by hydrolyzing laminarin also shows good antioxidant capacity, indicating that the enzyme has broad application prospect and significant practical value in the fields of antibacterial, biological medicine and the like.

The present disclosure relates to fungicidal combinations. The present disclosure relates to fungicidal combinations and compositions comprising laminarin and a contact fungicide for controlling fungal diseases in crops. The present disclosure further relates to a method of controlling phytopathogenic fungal diseases.

The invention relates to the technical field of feed and feed additives. The invention provides an astaxanthin-rich cladophora sp. SYSU-A2024-1, the classification name of the cladophora sp. SYSU-A2024-1 is Oedacladium sp.SYSU-A2024-1, the cladophora sp. SYSU-A2024-1 is preserved in China Center for Type Culture Collection on June 3, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20241138. The invention also provides a composition containing the cladophora sp. SYSU-A2024-1 and the chrysophyta laminarin, and an application of the composition in preparation of a low-fish-meal feed for aquatic animals. The trachinotus ovatus low-fish-meal feed containing the composition prepared by the invention can more comprehensively improve the lipid utilization rate of trachinotus ovatus, relieve oxidative stress and inflammatory injury, improve the liver health of fish bodies and remarkably improve the growth performance of the fish bodies through synergistic interaction, thereby ensuring the health and vitality of the fish bodies.

The present invention relates to a stable liquid composition comprising laminarin. The stable liquid compositions of the present invention are peculiar due to high loading of laminarin facilitated by the compositions. The present invention also relates to a process for the preparation of said composition / formulation and method of use thereof.

The invention relates to the technical field of biological medicines, in particular to an intestinal nutrition regulator containing laminarin and a preparation method of the intestinal nutrition regulator. The intestinal nutrition regulator containing the laminarin is prepared from the laminarin, probiotics and auxiliary materials through scientific proportioning and a unique process. The preparation method comprises the following steps: firstly, efficiently extracting polysaccharide from kelp through a process of combining ultrasonic-assisted enzyme extraction (UEE) and acid-assisted extraction (AAC); meanwhile, by exerting the synergistic effect of the laminarin, the silymarin extract and the resveratrol, the composition can effectively improve the intestinal flora structure, enhance the integrity of the intestinal barrier, inhibit the growth of pathogenic bacteria and regulate immune response. The intestinal nutrition regulator can be used for preparing food or health care products for regulating and / or improving intestinal flora balance of mammals, has good effects on preventing and improving intestinal related diseases such as intestinal dysfunction, irritable bowel syndrome and inflammatory bowel disease, and has wide market prospect and application value.

The present invention relates to a method for improving yield, disease management and post-harvest quality of a crop, a plant or an agricultural produce using laminarin or a composition comprising laminarin. The present invention also relates to combinations of laminarin and additional agrochemically active agents, such as fungicides for improving yield, disease management and post-harvest quality of a crop, a plant or an agricultural produce.

The present invention relates to a composition comprising water soluble actives such as laminarin. Particularly, the present invention relates to a stable composition comprising water soluble actives such as laminarin and a polyol. The present invention also relates to a process for the preparation of said composition and method of use thereof.

The invention provides laminarin, a lentinus edodes stem polysaccharide hydrolysate and application of the laminarin and the lentinus edodes stem polysaccharide hydrolysate in promotion of Weissella proliferation, and belongs to the technical field of microorganisms. The method comprises the following steps: mixing kelp powder with water, extracting, concentrating, carrying out alcohol precipitation by using ethanol with the final concentration of 18-22% v / v, dissolving the precipitate in water, and dialyzing to obtain laminarin; the method comprises the following steps: mixing lentinus edodes stem powder with water, extracting, concentrating, carrying out alcohol precipitation by using ethanol with the final concentration of 18-22% v / v, carrying out alcohol precipitation by using ethanol with the final concentration of 48-52% v / v, removing protein, hydrolyzing by using a hydrochloric acid solution, and dialyzing to obtain the lentinus edodes stem polysaccharide hydrolysate. The laminarin and lentinus edodes stem polysaccharide hydrolysate prepared by the invention is beneficial to the growth and reproduction of Weissella and can be used for preparing a preparation for promoting the proliferation of Weissella.

The invention belongs to the technical field of pharmaceutical preparations and transdermal delivery, and particularly relates to a medicine-adjuvant soluble microneedle patch based on laminarin and a preparation method of the medicine-adjuvant soluble microneedle patch. The medicine-adjuvant soluble microneedle patch adopts laminarin as a supporting material of a microneedle structure and a therapeutic agent with pharmacological activity. The molecular weight of the laminarin is 5 to 30 kDa; the medicine-adjuvant soluble microneedle patch further comprises an active medicine component, the mass ratio of the active pharmaceutical ingredient to the laminarin is 1: (1-50). The active medicine component comprises one or more of a cardiovascular treatment medicine, an anti-tumor medicine or an immunoregulation medicine. The drug-adjuvant soluble microneedle patch provided by the invention takes laminarin as a matrix material, has good biocompatibility, biodegradability, film-forming ability and natural pharmacological activity, and not only can be used as a microneedle supporting material, but also can play a synergistic therapeutic effect with a loaded drug.

The invention discloses an application of Laminarin in preparation of a medicine for treating cholestatic liver diseases. The invention reveals that the Laminarin can simultaneously inhibit the expression of inflammatory factors and promote liver regeneration for the first time, and fills the blank of the prior art.

The present application belongs to the technical field of skin care products, and particularly relates to a water-light needle skin care composition. A preservative composition is composed of p-hydroxyacetophenone and laminarin at a mass ratio of 1:3. When p-hydroxyacetophenone and laminarin are used at a mass ratio of 1:3, the growth of molds such as Aspergillus versicolor and Penicillium purpurescens and bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus can be inhibited, and the effect is synergistic. Compared with the use of p-hydroxyacetophenone alone, the inhibitory effect on various microorganisms can be improved, the resistance of the water-light needle skin care composition to external microorganisms can be improved, and the storage time of the water-light needle skin care composition can be prolonged. At the same time, the addition amount of the preservative component in the water-light needle skin care composition can be reduced, and the influence on human skin and health can be avoided.

This invention provides hydrolysates of kelp polysaccharides and shiitake stem polysaccharides, and their application in promoting the proliferation of *Vibrio sieboldii*, belonging to the field of microbial technology. The invention involves extracting and concentrating kelp powder with water, followed by alcohol precipitation with ethanol at a final concentration of 18-22% v / v. The precipitate is then dissolved in water and dialyzed to obtain kelp polysaccharides. Similarly, shiitake stem powder is extracted and concentrated with water, first precipitated with ethanol at a final concentration of 18-22% v / v, then with ethanol at a final concentration of 48-52% v / v to remove proteins, followed by hydrolysis with hydrochloric acid solution and dialyzed to obtain shiitake stem polysaccharide hydrolysates. The kelp polysaccharides and shiitake stem polysaccharide hydrolysates prepared by this invention promote the growth and reproduction of *Vibrio sieboldii* and can be used to prepare formulations that promote *Vibrio sieboldii* proliferation.

This invention relates to the field of bioengineering technology, specifically to an extracellular vesicle from kelp, its preparation method, and its applications. This invention provides a method for efficiently isolating extracellular vesicles with specific biological activities from kelp, and an application scheme for these vesicles to promote the proliferation, migration, and maintenance of stemness of large yellow croaker muscle stem cells under low serum conditions. This overcomes the problems of high cost and limited functionality of fetal bovine serum as a substitute in existing cell-cultured meat technologies, providing a novel culture medium supplement component that is stable in origin, low in cost, and has a clear effect.

The invention discloses an extraction method of laminarin. The method comprises the following steps: 1) pretreating wet kelp by adopting steam explosion, drying and crushing to obtain powder; 2) carrying out first extraction on the powder by adopting a first organic solvent, and carrying out solid-liquid separation to obtain a first precipitate; (3) carrying out secondary extraction on the first precipitate and an inorganic solvent, and carrying out solid-liquid separation to obtain supernate; 4, the supernate and a second organic solvent are subjected to third extraction and solid-liquid separation, a second precipitate is obtained, and the laminarin is obtained.By means of the extraction method, the extraction rate of the laminarin can be increased, the molecular weight of the laminarin can be reduced, and the uronic acid content can be increased.

The application belongs to the technical field of fishery culture, and provides a low fish meal compound feed with low oxygen tolerance, a preparation method and application thereof, the low fish meal compound feed contains the following raw materials: fish meal, soybean meal, soybean protein isolate, flour, chicken meal, cassava starch, fish oil, soybean oil, lecithin, calcium dihydrogen phosphate, compound vitamin, compound mineral substance, choline, vitamin C, DL-Met, Lys-HCL, Thr, astaxanthin and chrysoflagellate laminarin polysaccharide. Firstly, the compound plant protein such as soybean meal and soybean protein isolate and chicken meal are used to replace the traditional fish meal in the application, the amino acid composition is balanced, the nutritional proportion of the compound feed is optimized, and the proportion of fish meal is reduced to 20%; secondly, the compound antioxidant such as astaxanthin and chrysoflagellate laminarin polysaccharide is added in the compound feed, and the fish body antioxidant capacity can be significantly improved.

The invention discloses a glucanase SDuLam55A gene, a recombinant glucan rSDuLam55A enzyme, a method and an application of the recombinant glucan rSDuLam55A enzyme. The nucleotide sequence of the StuLam55A gene is as shown in SEQ ID NO: 1, the StuLam55A gene is derived from Stropharia rugoso-annulata, and the coded recombinant glucan rStuLam55A enzyme (96kDa) has strict substrate specificity and only aims at hydrolysis of beta-1, 3-glucosidic bonds in laminarin. The invention provides a method for cloning the gene from stropharia rugoso-annulata hyphae and a process for efficiently preparing the rSDuLam55A enzyme through a recombinant expression system. The enzyme has remarkable application value in the fields of penicillium growth inhibition, agricultural waste treatment, food preservation and the like, and high stability, strict substrate specificity and efficient catalytic characteristics of the enzyme provide a safe and environment-friendly novel enzyme preparation solution for related industries.

The present application provides a kind of laminarin-western radix astragali glue-silk microfiber medical catheter and its preparation method, and the medical catheter preparation solution contains the following components per 100ml: laminarin 1-10g, aqueous solution 10-100mL, 10% radix astragali glue solution 2-30mL, silk microfiber 1-10g.The material of the present application is low in price, and the preparation method is simple and easy to operate, which avoids the cytotoxicity side effects caused by the use of toxic crosslinking agent, has good biocompatibility, and at the same time, the prepared biomimetic gel catheter has good porosity, mechanical strength and good flexibility, is suitable for blood vessels, peripheral nerve catheter and other applications, and is easy to metabolize in vivo.

The present invention relates to a composition comprising a water-soluble active substance, such as laminarin. In particular, the present invention relates to a stable composition comprising a water-soluble active substance, such as laminarin, and a polyol. The present invention also relates to a process for preparing said composition and methods of use thereof.

An active polysaccharide compound nutrient for boosting immunity and relieving fatigue, including 10-12 parts by weight of lentinan, 8-10 parts by weight of laminarin, 5-8 parts by weight of Lycium barbarum, 10-15 parts by weight of Mytilus edulis extract, 10-15 parts by weight of organic selenium protein powder, 8-10 parts by weight of walnut kernel, 3-5 parts by weight of black soybean powder, 2-3 parts by weight of Panax quinquefolius extract, 1-2 parts by weight of Schisandra chinensis, 1-2 parts by weight of Ophiopogon japonicus, 1-2 parts by weight of nutritional yeast, 5-10 parts by weight of L-arabinose, 5-8 parts by weight of xylitol and 0.2-0.3 part by weight of pancreatin. The active polysaccharide compound nutrient is prepared by subjecting raw materials to crushing, mixing, stirring, dissolving with deionized water, concentrating and drying under reduced pressure.

The application provides a fluorescent sensor material for detecting metal ions in seawater and a preparation method and application thereof. The fluorescent sensor material is a complex formed by simultaneously complexing 2,2'-dipyridyl ligand and laminarin ligand with trivalent terbium ions, and a structural formula of the fluorescent sensor material is shown as formula (I). The application also provides a preparation method of the fluorescent sensor material. The preparation method comprises the following steps: mixing an ethanol solution of terbium salt with an ethanol solution of 2,2'-dipyridyl, stirring at room temperature, adding an aqueous solution of laminarin, and continuously stirring at room temperature. The fluorescent sensor material has stable structure, organic complex of 2,2'-dipyridyl, laminarin and rare earth metal terbium ions, strong light emission, good biocompatibility, good dispersibility, simple preparation process, green environmental protection, high selectivity to metal ions in seawater, and is widely used for detecting metal ions in seawater, and is especially suitable for detecting trivalent iron ions in seawater.

This invention discloses a kelp fucoidan, its preparation method, and its application. The kelp fucoidan includes LF3-1 and / or LF4-1. The main chain of LF3-1 is composed of alternating →3)-α-L-Fucp-(1→ and →3)-β-D-Galp-(1→). The fucose residues on the main chain of LF3-1 are sulfated at the C-4 position or connected to a first branch, which is composed of →4)-α-L-Fucp-(1→. The galactose residues on the main chain of LF3-1 are sulfated at the C-6 position or connected to a second branch, which is composed of →2,3)-β-D-Manp-(1→ and β-D-Galp-(1→). The sulfate content of LF3-1 is 26.3%, and the weight-average molecular weight of LF3-1 is 305. LF4-1 has a molecular weight of 32.7% and a weight-average molecular weight of 182.1 kDa. Its main chain consists of alternating links of →3)-α-L-Fucp-(1→ and →6)-β-D-Galp-(1→). The fucose in the main chain of LF4-1 is sulfated at C-4 or has a third branch, which is composed of →4)-α-L-Fucp-(1→. The galactose in the main chain of LF4-1 is sulfated at C-3 or has a fourth branch, which is composed of →2,4)-β-D-Manp-(1→ and β-D-Galp-(1→). LF4-1 effectively improves blood lipids and insulin resistance, promotes lipid oxidation and glucose metabolism gene expression, and thus plays a role in weight loss and lipid reduction.