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Method for extracting laminarin from kelp

A technology of kelp polysaccharide and kelp, which is applied in the field of polysaccharide preparation, can solve the problems of restricting the deep processing and application of kelp polysaccharide, the reduction of polysaccharide biological activity, and the time-consuming enzyme extraction method, so as to avoid the decrease of kelp polysaccharide content, reduce energy consumption, The effect that the reaction conditions are easy to control

Inactive Publication Date: 2014-07-23
广西还珠海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are some documents or reports on the extraction of laminaria polysaccharides. The traditional methods are mainly acid extraction, alkali extraction, enzyme extraction, etc. These extraction methods have certain defects: acid extraction can cause polysaccharides Hydrolysis and alkali extraction lead to a decrease in the biological activity of polysaccharides. Enzyme extraction is time-consuming. Industrial production requires a large amount of enzymes and high costs. The method of hot water extraction and alcohol precipitation is simple, but time-consuming and energy-consuming
There are deficiencies in the existing methods, which restrict the deep processing and application of laminarin to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for extracting laminarin from kelp, comprising the steps of:

[0033] a. Raw material processing: take 10kg of fresh kelp, dry it, and crush it to 40 meshes;

[0034] b. Ultrasonic treatment: add 10 times of pure water to the pulverized kelp powder, ultrasonic treatment for 20 minutes, the action temperature is 80°C, and the power is 300W. After completion, filter and collect the filtrate;

[0035] c. Enzymolysis: Add 1.5% trypsin by weight of the slurry, stir evenly, enzymolyze at 45°C for 1 hour, adjust the pH to 3.0, then add pepsin with 2.5% by weight of the slurry, stir evenly, enzymolyze at 35°C for 3 hours, then boil water Bath for 10min, filter, and collect the filtrate;

[0036] d. Decolorization: Add 95% ethanol with a mass concentration equivalent to 2 times the weight of the filtrate to the filtrate obtained in step c, let stand at 4°C for 4h, filter, add activated carbon with 0.5% of the weight of the filtrate to the filtrate, and incubate at 80°C...

Embodiment 2

[0039] A method for extracting laminarin from kelp, comprising the steps of:

[0040] a. Raw material processing: take 10kg of dried kelp and grind it to 60 mesh;

[0041] b. Ultrasonic treatment: add 20 times of pure water to the pulverized kelp powder, ultrasonic treatment for 40 minutes, the action temperature is 70 ℃, the power is 350W, filter after completion, and collect the filtrate;

[0042] c. Enzymolysis: Add 2.5% trypsin by weight of slurry, stir evenly, enzymolyze at 35°C for 3 hours, adjust the pH value to 5.0, then add pepsin with 1.5% by weight of slurry, stir evenly, enzymolyze at 45°C for 1 hour, then boil water Bath for 20min, filter, and collect the filtrate;

[0043] d. Decolorization: Add 95% ethanol with a mass concentration equal to twice the weight of the filtrate to the filtrate obtained in step c, let it stand at 4°C for 4h, filter, add activated carbon with 0.5% of the weight of the filtrate to the filtrate, and incubate at 80°C for 35min Carry out...

Embodiment 3

[0046] A method for extracting laminarin from kelp, comprising the steps of:

[0047] a. Raw material processing: take 10kg of fresh kelp, dry it, and crush it to 60 mesh;

[0048] b. Ultrasonic treatment: add 15 times of pure water to the pulverized kelp powder, ultrasonic treatment for 30 minutes, the action temperature is 85°C, the power is 200W, after completion, filter and collect the filtrate;

[0049] c. Enzymolysis: Add 2.0% trypsin by weight of the slurry, stir evenly, enzymolyze at 40°C for 2 hours, adjust the pH to 4.0, then add pepsin with 2.0% by weight of the slurry, stir evenly, enzymolyze at 40°C for 2 hours, then boil water Bath for 15min, filter, and collect the filtrate;

[0050] d. Decolorization: add ethanol with a mass concentration of 95% equivalent to 2 times the weight of the filtrate to the filtrate obtained in step c, let stand at 4°C for 4h, filter, add activated carbon with 0.5% of the weight of the filtrate in the filtrate, and incubate at 80°C f...

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Abstract

The invention discloses a method for extracting laminarin from kelp. The method comprises the following steps: raw material treatment, ultrasonic treatment, enzymolysis, destaining, deproteinization and drying to obtain the product. Compared with the existing trichloroacetic acid precipitation method, savage method and the like, the method has the advantages that laminarin is high in yield and high in content.

Description

technical field [0001] The invention relates to a method for preparing polysaccharides, in particular to a method for extracting laminaria polysaccharides from kelp. Background technique [0002] Kelp (Laminaria Japonica Aresch) is a large seaweed belonging to the genus Laminaria of the family Laminaria. Kelp is rich in a variety of functional components and has extremely high edible and medicinal value. Its medicinal value has been recorded in traditional medical books such as "Compendium of Materia Medica", "Shen Nong's Materia Medica" and "Food Materia Medica". Studies have shown that the various biological functions of kelp are mostly related to the physiologically active polysaccharides in kelp, such as immune regulation, anti-tumor, anti-coagulation, lowering lipids, lowering blood sugar, and anti-oxidation. [0003] At present, there are some documents or reports on the extraction of laminaria polysaccharides. The traditional methods are mainly acid extraction, alkal...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 归三岷
Owner 广西还珠海洋生物科技有限公司
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