Method for quickly detecting melamine in milk sample based on nanogold

A technology of melamine and nano-gold, which is applied in the preparation of test samples, measurement of color/spectral characteristics, and material analysis by observing the impact on chemical indicators, etc., which can solve the problem of high detection operation requirements, complex detection process, and detection cost. Advanced problems, to achieve the effect of simple and feasible preparation method, low detection cost and good stability

Inactive Publication Date: 2010-06-02
FUZHOU UNIVERSITY
View PDF7 Cites 35 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems in the prior art that the detection operation requirements for melamine are high, the detection process is complicated, the detection cost is high, the detection time is long, and t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly detecting melamine in milk sample based on nanogold
  • Method for quickly detecting melamine in milk sample based on nanogold
  • Method for quickly detecting melamine in milk sample based on nanogold

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0041] 1. Preparation of 13nm Nano Gold

[0042] Using sodium citrate to reduce chloroauric acid, heat 50mL of 1mM chloroauric acid solution to boiling, and then quickly add 5mL of 38.8mM trisodium citrate, reflux for 15min, stop heating, and cool to room temperature while stirring. The maximum absorption wavelength of the gold nanoparticles prepared by this method is 520nm, and the color of the solution is wine red.

[0043] 2. Application of Nano Gold to the Detection of Melamine

Example Embodiment

[0044] Example 1 Detection of melamine in liquid milk by nano gold

[0045] Weigh 11 1.6g portions of liquid milk containing 0ppm, 0.48ppm, 0.95ppm, 1.90ppm, 3.80ppm, 5.70ppm, 7.60ppm, 9.50ppm, 11.4ppm, 13.3ppm, 15.2ppm melamine to a 7ml centrifuge tube and add 0.72 mL of trichloroacetic acid (10%) and 1 mL of chloroform, trichloroacetic acid and chloroform. After ultrasonic treatment, centrifugal separation (200W ultrasonic treatment for 15 minutes followed by 13000 rpm centrifugation for 6 minutes) to remove protein and fat, and the supernatant obtained with 1M sodium carbonate solution Adjust its pH to pH 8.0, and then further remove the precipitate by centrifugation (3000rpm for 1 min). Take the supernatant for melamine detection as follows: Take 0.1 mL of nano gold solution and add 0.2 mL of different melamine mass ratios The liquid milk supernatant is mixed uniformly, and after the color reaction is placed for 0.5min, UV-visible absorption spectrum analysis and visual color...

Example Embodiment

[0046] Example 2 Detection of melamine in milk powder by nano gold

[0047] Weigh 13 parts of 0.6g milk powder containing 0ppm, 0.84ppm, 1.93ppm, 4.2ppm, 12.6ppm, 21ppm, 29.4ppm, 37.8ppm, 46.2ppm, 54.6ppm, 63ppm, 71.4ppm, 79.8ppm melamine to a 7ml centrifuge tube Add 2mL of secondary water to dissolve, add 2mL of trichloroacetic acid (10%) and 1.2mL of chloroform, and centrifuge after ultrasonic treatment (200W ultrasonic treatment for 15 minutes and then centrifuge at 13000rpm for 6 minutes) to remove protein and fat, and the resulting supernatant The pH of the solution was adjusted to pH 7.0 with 1M sodium carbonate solution, and the precipitate was further removed by centrifugal separation (1 min at 3000 rpm). The supernatant was taken for melamine detection as follows: take 0.1 mL of nano gold solution, Add 0.2mL milk powder supernatants with different mass ratios of melamine, mix them evenly, and carry out UV-visible absorption spectrum analysis after the color reaction for ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides a method for quickly detecting melamine in a milk sample based on nanogold, which aims to solve the problems of the high operation requirement, complex detection process, high cost, long time, incapacity of meeting a lot of food test requirements and the like on the detection of the melamine. The method comprises the following steps: performing pretreatment on the milk sample and adding the pretreated milk sample into a nanogold solution; and adopting visual colorimetry to compare the color of the nanogold added with the sample solution with the color in a color standard series and then qualitatively judging whether the milk contains the melamine or not. The color standard series is prepared through the following steps: adding the pretreated milk samples in an identical volume and containing the melamine of different concentrations into the nanogold solution, wherein the content of the nanogold in each sample is consistent; and after color development reaction, obtaining the color standard series. The method has the advantages of low cost, simple and convenient operation and capacity of visually and quickly detecting the melamine in the milk.

Description

technical field [0001] The invention belongs to the technical field of melamine detection, and more specifically relates to a method for rapidly detecting melamine in milk samples based on nano-gold. Background technique [0002] Melamine is a triazine nitrogen-containing heterocyclic organic compound, referred to as triamine, commonly known as melamine and protein essence. As a widely used organic chemical raw material, melamine is mainly used as a raw material for the production of melamine formaldehyde resin (MF). In addition, melamine is also used as a flame retardant, water reducer, formaldehyde cleaner, etc. Since the food industry mainly uses the Kjeldahl method to determine the content of nitrogen atoms to indirectly calculate the protein content in food, in recent years, melamine has been added to food and feed by criminals because of its high nitrogen content (66%) to increase The apparent protein content of the product. Studies have shown that melamine is slight...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78G01N21/33G01N1/34
Inventor 郭良洽钟坚海
Owner FUZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap