Method for quickly detecting melamine in milk sample based on nanogold
A technology of melamine and nano-gold, which is applied in the preparation of test samples, measurement of color/spectral characteristics, and material analysis by observing the impact on chemical indicators, etc., which can solve the problem of high detection operation requirements, complex detection process, and detection cost. Advanced problems, to achieve the effect of simple and feasible preparation method, low detection cost and good stability
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[0041] 1. Preparation of 13nm gold nanoparticles
[0042] Using the method of reducing chloroauric acid with sodium citrate, heat 50mL of 1mM chloroauric acid solution to boiling, then quickly add 5mL of 38.8mM trisodium citrate, after reflux reaction for 15min, stop heating, and naturally cool to room temperature under stirring , the maximum absorption wavelength of the gold nanometer prepared by this method is 520nm, and the color of the solution is wine red.
[0043] 2. The application of gold nanoparticles to the detection of melamine
Embodiment 1
[0044] Embodiment 1 Nano gold detects melamine in liquid milk
[0045] Weigh 11 parts of 1.6g liquid milk containing 0ppm, 0.48ppm, 0.95ppm, 1.90ppm, 3.80ppm, 5.70ppm, 7.60ppm, 9.50ppm, 11.4ppm, 13.3ppm, 15.2ppm melamine respectively into a 7ml centrifuge tube, add 0.72mL trichloroacetic acid (10%) and 1mL chloroform Trichloroacetic acid and chloroform, centrifugal separation after ultrasonic treatment (13000rpm centrifugation 6 minutes after 200W ultrasonic treatment 15 minutes) remove protein and fat, the supernatant obtained is washed with 1M sodium carbonate solution Adjust its pH to pH 8.0, then further remove the precipitate by centrifugation (3000rpm centrifugation for 1min), take the supernatant and carry out melamine detection according to the following method: take 0.1mL nano gold solution, add 0.2mL different melamine mass ratio The liquid milk supernatant, mixed evenly, placed for 0.5min after the color reaction, carried out UV-visible absorption spectrum analysis ...
Embodiment 2
[0046] Embodiment 2 Nano-gold detects melamine in milk powder
[0047] Weigh 13 parts of 0.6g milk powder containing 0ppm, 0.84ppm, 1.93ppm, 4.2ppm, 12.6ppm, 21ppm, 29.4ppm, 37.8ppm, 46.2ppm, 54.6ppm, 63ppm, 71.4ppm, 79.8ppm of melamine to a 7ml centrifuge tube , add 2 mL of secondary water to dissolve, add 2 mL of trichloroacetic acid (10%) and 1.2 mL of chloroform, centrifuge after ultrasonic treatment (200W ultrasonic treatment for 15 minutes and then centrifuge at 13,000 rpm for 6 minutes) to remove protein and fat, and the supernatant The pH of the liquid was adjusted to pH 7.0 with 1M sodium carbonate solution, and then the precipitate was further removed by centrifugation (centrifugation at 3000rpm for 1min), and the supernatant was taken for melamine detection according to the following method: take 0.1mL of nano-gold solution, Add 0.2mL of milk powder supernatant with different mass ratios of melamine, mix evenly, place for 0.5min for color reaction, and then conduct ...
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