The invention relates to the field of tobacco floating
seedling and aims to provide a method for improving the
germination rate of tobacco floating
seedling seeds and strengthening seedlings. According to the method, a
thallus of
plant root
endophytic fungus piriformospora indica, which is cultured by using a
liquid medium, and a culture filtrate are uniformly mixed with a tobacco
seedling substrate, and the
germination rate of the tobacco floating seedling seeds is improved and the growth and stress tolerance of tobacco seedlings are promoted by using the
thallus of
piriformospora indica and the substances released by the
thallus in the culture filtrate. The method provided by the invention is simple, convenient and feasible, and according to the method, the
piriformospora indica is cultured by using a conventional method, standard rules are used for performing substrate mixing, tray filling,
sowing, floating seedling, seedling exercising and
transplantation, and the manner of inoculating the piriformospora indica is matched with the rules for floating seedling. By using the method provided by the invention, the
germination rate of the tobacco seeds can be improved; root systems of tobacco seedlings are assisted to tolerate low-
oxygen stress, so that the root systems of the tobacco seedlings are developed and the root
dry weight is increased; the roots are assisted to absorb nutrients, so that the growth of the tobacco seedlings is promoted, the stems of the tobacco seedlings are thickened, the
diameter of the stems is increased, and the
plant height is increased; and the
survival rate of the tobacco seedlings after
transplantation is improved.