The invention discloses a separation and enrichment determination method for Ir, Rh, Pt, Pd and Au in a secondary resource material. The method includes the following steps that 1, samples are dissolved, and after high-temperature firing carbon removal, a Na2O2+NaCO3 mixed fluxing agent is adopted for fusion in a muffle furnace of 750 DEG C; 2, a test solution is oxidized, an appropriate quantity of sodium chlorate solution is added in a dissolving solution, boiling is performed, and Ir in the solution is oxidized so that the valence state can be made stable; the solution is adjusted to be at the appropriate volume, and the volume concentration of hydrochloric acid reaches 50%; 3, tellurium coprecipitation separation is carried out; 4, determination is carried out, an inductively coupled plasma optical emission spectrometry (ICP-AES) is used for determining the content of the Ir, Rh, Pt, Pd and Au in the solution obtained in the third step at the same time. The accurate and fast separation and enrichment determination method is specifically used for the Ir, Rh, Pt, Pd and Au with the content scope being 0.001%-5% in the secondary resource material such as waste and metallurgical middle materials formed in the using process of various precious metal products. The method is also suitable for separation and enrichment determination of Ir, Rh, Pt, Pd and Au single elements in the secondary resource material.