Method for improving germination rate of tobacco floating seedling seeds and strengthening seedlings
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A floating seedling raising and germination rate technology, applied in the directions of botanical equipment and methods, germination equipment, soilless cultivation, etc., can solve the problems of low oxygen stress, drought resistance and low germination rate for aquatic rooting of tobacco seedlings, so as to help root absorption. The effect of nutrition, stem thickening and root development
Inactive Publication Date: 2015-04-29
ZHEJIANG UNIV +1
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Problems solved by technology
[0007] The technical problem to be solved by the present invention is, aiming at the current low germination rate of tobacco floating seedlings, the water rooting of tobacco seedlings under low oxygen stress and weak drought resistance after transplanting, the mycorrhized tobacco seedlings technology from branch mycorrh
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Embodiment 1
[0048] Floating seedlings were simulated under laboratory conditions, and the effect of inoculating Pyromorpha indica liquid on floating seedlings of tobacco was tested.
[0049] 1. Cultivation of Piromorpha indica with artificial medium
[0050] Prepare PD and PDA according to routine, each liter medium contains 200g peeled and boiled potatoes and 20g glucose, pH is 6.5, and is sterilized at 121°C for 20 minutes by high-pressure steam. For liquid culture, fill 200ml of medium in a 500-ml glass Erlenmeyer flask and culture on a shaker at a shaking speed of 220rpm. Take the fresh mycelia agar block grown on PDA, transfer it to PD for 4 days, then divide the bacterial solution into PD and expand the culture for 14 days. 15g and about 150ml of culture filtrate. The fresh weight of 100g of thalline in the obtained bacterial liquid corresponds to 1L of culture filtrate, and the number of spores reaches about 2.5×10 per liter. 9 indivual.
[0051] 2. Mixing Pyromorpha indica liq...
Embodiment 2
[0063] Under the conditions of greenhouse seedling cultivation and field plots, the effect of inoculating Pyromorpha indica liquid on tobacco seedling cultivation and transplanting was tested.
[0064] 1. cultivate Indian piriformis with embodiment 1 with artificial medium
[0066] On February 11, 2014, in the seedling shed of the nursery farm in Jichang Town, Dafang County, Bijie City, Guizhou Province, the liquid of Pyromorpha indica was mixed with the tobacco seedling substrate (produced by Zunyi Liangu Agricultural Technology Co., Ltd.).
[0067] (1) Mix and shake about 300 g of thalline and 3 L culture filtrate of Pyromorpha indica culture, and mix with about 30 kg of matrix, that is, the resulting bacterium liquid matrix mixture contains about 1% of thalline and 10% of culture filtrate.
[0068] (2) Shake about 60g of thalline and 600ml culture filtrate of Piriformis indica culture, and m...
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Abstract
The invention relates to the field of tobacco floating seedling and aims to provide a method for improving the germination rate of tobacco floating seedling seeds and strengthening seedlings. According to the method, a thallus of plant root endophytic fungus piriformospora indica, which is cultured by using a liquid medium, and a culture filtrate are uniformly mixed with a tobacco seedling substrate, and the germination rate of the tobacco floating seedling seeds is improved and the growth and stress tolerance of tobacco seedlings are promoted by using the thallus of piriformospora indica and the substances released by the thallus in the culture filtrate. The method provided by the invention is simple, convenient and feasible, and according to the method, the piriformospora indica is cultured by using a conventional method, standard rules are used for performing substrate mixing, tray filling, sowing, floating seedling, seedling exercising and transplantation, and the manner of inoculating the piriformospora indica is matched with the rules for floating seedling. By using the method provided by the invention, the germination rate of the tobacco seeds can be improved; root systems of tobacco seedlings are assisted to tolerate low-oxygen stress, so that the root systems of the tobacco seedlings are developed and the root dry weight is increased; the roots are assisted to absorb nutrients, so that the growth of the tobacco seedlings is promoted, the stems of the tobacco seedlings are thickened, the diameter of the stems is increased, and the plant height is increased; and the survival rate of the tobacco seedlings after transplantation is improved.
Description
technical field [0001] The invention belongs to the field of tobacco floating seedling cultivation and the field of microbial application, and specifically relates to a method for improving the germination rate and strong seedlings of tobacco floating seedling cultivation by using the thalline of plant root endophytic fungus Pyromorpha indica and the substance released in the culture filtrate. Background technique [0002] At present, the intensive production of tobacco is mainly based on seedling cultivation and transplanting, and the intensive cultivation of tobacco seedlings is mainly based on floating seedling cultivation. The temperature in the intensive seedling raising period in most tobacco-growing areas across the country is low, which leads to long germination time and low germination rate of seeds. When floating seedlings, the density of tobacco seedlings is high, and the oxygen demand of the root system is large, while the liquid surface of the nutrient solution ...
Claims
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