11results about How to "Well developed root system" patented technology

The invention discloses a cubilose fruit grafting seedling raising method, and belongs to the technical field of fruit tree cultivation. The method comprises six aspects of stock planting field establishment, stock seedling raising, stock water and fertilizer management, grafting, grafted seedling water and fertilizer management and grafted seedling field management. A special cutter tool for cubilose fruit grafting is adopted, an L-shaped section can be formed, the section is smooth and good in integrating degree, and the grafting survival rate and the grafting efficiency can be improved. According to the invention, the seedling raising grids are used for stock seedling raising, so that the seedling raising cost can be saved, and the space utilization rate can be improved. According to the method, the thorn-free huanglong and the argentodoxa cuneata are adopted as the stocks, the root systems are developed, the morbidity of root-knot nematodes can be reduced, the grafting survival rate and the growth vigor are improved, and meanwhile the canker resistance of plants is improved; the quality of the rootstock is guaranteed, the grafting growth vigor is improved, bud germination is promoted, and fruit bearing is carried out in advance by optimizing the length, the period and the number of bud points of the rootstock.

This invention belongs to the field of plant tissue culture technology, specifically relating to a tissue culture rapid propagation medium and method for rooting *Carex latifolia*. The invention provides a tissue culture rapid propagation medium for rooting *Carex latifolia*, comprising a rooting medium; the rooting medium uses 1 / 2 MS as the basal medium and further includes NAA 0.8 mg·L⁻¹, IBA 1.0 mg·L⁻¹, sucrose 30 g·L⁻¹, and agar 7 g·L⁻¹, with a pH of 5.8-6.0. The invention also provides a method for direct rooting of *Carex latifolia* through tissue culture rapid propagation. Aseptic seedlings of *Carex latifolia* have their outer, weaker leaves removed, and the original root system cut off, retaining 2-3 cm of the above-ground portion. These seedlings are then inoculated into the rooting medium for rooting culture to obtain rooted seedlings. After hardening off the rooted aseptic seedlings, the well-rooted *Carex latifolia* seedlings are transplanted into a substrate to form new, complete plants. The method for rapid propagation of broadleaf sedge by tissue culture provided by this invention has the characteristics of high rooting rate, large average number of roots, well-developed root system, high transplant survival rate, and robust regenerated seedlings.

The present application relates to the technical field of breeding and seedling raising of forest trees, and discloses a method for realizing in vitro regeneration of Rosa roxburghii by hypocotyl culture, which comprises the following steps: taking seedling germinated from cold storage of Rosa roxburghii seeds as material, removing cotyledon and root, cutting hypocotyl segments with a length of 0.6-1.5 cm as explants, and sterilizing the explants; inoculating the sterilized explants into primary induction medium, and carrying out dark culture until callus is generated and primary adventitious buds are induced; inoculating the cultured callus into differentiation medium, and carrying out dark culture until secondary adventitious buds are regenerated; inoculating the obtained secondary adventitious buds with a height of 1.5-2.5 cm into rooting medium until rooted plants are obtained; and hardening the obtained rooted plants, and then transplanting them into mixed substrate for culture.

The application discloses a water quality purifying device for water body treatment, and relates to the technical field of water quality purification, which comprises a water quality purifier arranged in a river channel, wherein the water quality purifier comprises two groups of first frame bodies and second frame bodies arranged in parallel and symmetrically; the first frame bodies and the second frame bodies are fixedly connected through two groups of connecting frames arranged in parallel and symmetrically; the first frame bodies, the second frame bodies and the connecting frames enclose a purifier frame; wherein a plurality of groups of biological filler supports are arranged in an array mode in the purifier frame; an adjusting module is further arranged in the purifier frame, and the adjusting module is used for adjusting the spacing of the biological filler supports; the spacing of the biological filler supports of each layer can be adjusted in real time through the adjusting module, so that the spacing of the biological filler supports of each layer is larger; therefore, the water flow can flow in the gaps between the biological filler supports, the blocking of the water flow by the biological filler supports is reduced, the stability of the water quality purifier in the river channel is improved, and the water quality purifier is prevented from being washed away or washed out.

The present application relates to the technical field of plant tissue culture, and provides a tissue culture method for promoting germination of Zingiber zerumbet Smith, wherein Zingiber zerumbet Smith tubers are used as explants, and after being subjected to disinfection treatment, the explants are inoculated into an induction medium, a proliferation medium and a rooting medium in sequence, and then are subjected to acclimatization, seedling raising and transplanting, so that finished Zingiber zerumbet Smith seedlings are obtained. The germination rate, the proliferation coefficient and the rooting rate of the Zingiber zerumbet Smith tubers can be significantly improved by using the culture media which are optimized and adjusted in the technical scheme of the present application. In addition, the reasonable application of foliar fertilizer and fungicide after transplantation in the present application can further improve the growth vigor and the disease resistance of Zingiber zerumbet Smith seedlings, and ensure the survival rate of the seedlings, thereby providing reliable technical support for rapid propagation and large-scale planting of Zingiber zerumbet Smith.

The application discloses a multifunctional Bacillus mobilis ASH9-3 and application thereof, and belongs to the technical field of microorganisms. The Bacillus mobilis ASH9-3 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 32327. The strain has the functions of antagonizing four soil-borne diseases such as tomato early blight, producing protease, producing indole acetic acid (IAA), decomposing inorganic phosphorus and fixing iron ions. The fermentation liquor of the strain is applied to soil, so that the occurrence rate of soil-borne diseases can be significantly reduced, the content of soil organic matter and available nutrients can be increased, and the yield of peppers can be increased by 32.4%. The application fills the blank of multifunctional microbial agents in the prior art, and provides an efficient solution for soil-borne disease prevention and control and sustainable management of soil.

The application provides a method for inducing rapid rooting of lily bulbils and application thereof, and the lily bulbil (diameter>1.0cm) is used as material, the outer scale is peeled off after surface disinfection and low-temperature pretreatment, the peeled-off outer scale is placed in sterile sawdust with humidity of 15%-25% and cultured at 26 DEG C in dark, and the lily bulbil can be rooted. Compared with a control, the lily bulbil obtained by the method has developed root system, early root system germination, and the number and length of roots are obviously increased, the low-temperature pretreatment time is short, the culture period is short, and a large amount of lily root system can be rapidly obtained in about 10 weeks, and the lily root system can be applied to preparation of medicines, foods and cosmetics resisting melanin synthesis and inflammation reaction, and the method can also realize rapid propagation of lily and provide a new selection, and has important significance. The material is easy to obtain, the method is non-tissue culture, simple and convenient to operate, and can be produced on a large scale, and has good application prospect.

The invention relates to the technical field of forest tree genetic breeding and propagation, and particularly discloses a seedling raising method for improving the grafting survival rate of phoebe bournei. Comprising the following steps: S1, cultivating phoebe bournei cutting seedlings as rootstocks, and keeping the rootstocks in a robust state for later use; s2, semi-lignified branches growing in the current season are collected from the excellent phoebe bournei stock plants to serve as scions; s3, performing disinfection and pretreatment on the scions to prepare scion sections with wedge-shaped notches; s4, cutting off and cutting the stock, and combining the scions treated in the step S3 with the stock to enable cambiums to be aligned; s5, sealing and fixing the grafting joint part by using a binding belt material; s6, performing stage moisturizing and light and temperature regulation and control on the grafted plants. The seedling raising method can be applied to the fields of efficient germplasm resource utilization, artificial forest and landscape forest construction and the like, and has the advantages that the grafting survival rate is high and stable, the interface healing speed is high, the quality is good, and nursery stocks grow robustly and neatly.

This invention discloses a method for rapid propagation and seedling cultivation of *Gynostemma pentaphyllum* seedlings. Healthy, disease-free *Gynostemma pentaphyllum* plants are selected. Young leaves are washed to remove surface impurities and then disinfected. The disinfected explants are cut into pieces and inoculated into an adventitious bud induction medium to induce callus tissue. The induced callus tissue is then transferred to an adventitious bud differentiation medium to differentiate adventitious buds. The differentiated adventitious buds are then cut off of their leaves, retaining the stem segments with nodes, and inoculated into a proliferation medium. Proliferating buds with good growth and large, thick leaves are inoculated into a rooting medium. The rooted seedlings are then placed in a greenhouse for hardening off. The hardened rooted seedlings are then cleaned of the root culture medium before further cultivation. The tissue-cultured rooted seedlings produced by this method are robust, red in color, and have well-developed root systems. They exhibit high survival rates after cultivation and have a short cultivation time, providing technical support for the large-scale, standardized production of *Gynostemma pentaphyllum* and possessing broad application prospects.

The application belongs to the technical field of plant genetic engineering, and discloses a gene for promoting adventitious root development and application thereof. EuWOX11 The application specifically discloses the application of the gene, a recombinant expression vector containing the gene or an engineered bacterium containing the gene in at least one of promoting adventitious root generation, adventitious root elongation, increasing the number of adventitious roots, increasing the biomass of adventitious roots, and promoting the growth of an aboveground part; the application in at least one of improving the rooting characteristics of woody plants, shortening the rooting cycle of cuttage, increasing the rooting rate of cuttage, increasing the survival rate of cuttage, and increasing the efficiency of asexual reproduction; and a method for obtaining a plant with improved adventitious root development. The transgenic plant obtained by overexpressing the gene has a more developed root system, a more vigorous aboveground part, and stronger adaptability after planting, and can significantly improve the seedling raising efficiency and seedling quality and reduce the production cost, and has important application value and economic value in the molecular breeding and industrial breeding of eucommia ulmoides and populus tomentosa.

This invention relates to the field of Torreya grandis seedling technology, specifically to a method for cultivating Torreya grandis seedlings, comprising the following steps: S1, greenhouse germination; S2, independent sowing; S3, transplanting after removal from the greenhouse; S4, secondary cultivation in the greenhouse. By precisely controlling the temperature, humidity, and cycle of greenhouse germination, the germination rate of Torreya grandis seeds can be stably maintained at over 85%, and the germination time can be shortened. By using independent container sowing, combined with a specific ratio of nutrient soil and light management, the cultivated seedlings are robust and have well-developed root systems, effectively improving the transplant survival rate. After transplanting, reasonable fertilization and combined with secondary cultivation in the greenhouse in mid-to-late September, the greenhouse conditions are used to extend the growing season, so that the rootstock can reach the grafting standard in March-April of the following year. Compared with traditional methods, the seedling cultivation cycle is shortened by 6-10 months.