49results about How to "Improve resilience to adversity" patented technology

The invention relates to trichoderma asperellum chlamydospore powder as well as a preparation method thereof and an application. The preparation method of the trichoderma asperellum chlamydospore powder comprises the following steps: conservatively culturing arsenic-resisting activity, purifying, culturing shake-flask seeds, culturing fermented seeds, controlling the fermentation and post-processing. The trichoderma asperellum chlamydospore is high in adversity resistance, the powder of the trichoderma asperellum chlamydospore is slightly subjected to the ambient temperature, acidicity, alkalinity and the like, and the influence of different arsenic concentrations in the environment on the sprouting of the chlamydospore is not obvious. The trichoderma asperellum chlamydospore has strong vitality, the survival time of the trichoderma asperellum chlamydospore is long, the volatilization of arsenic in the soil can be obviously promoted, the total quantity of the arsenic in the soil can be reduced, and the soil polluted by the arsenic can be effectively repaired. By adopting the method, the time for producing a great amount of trichoderma asperellum chlamydospore can be greatly shortened, the production cost of the trichoderma asperellum chlamydospore can be remarkably reduced, and the popularization of the technology can be favored.

The invention discloses a compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and a preparation method thereof. The compound biotic insecticide is mainly mixed through the fermentation liquor of bacillus thuringiensis and the meristem of the verticillium lecanii or blastospore and the aconite alkaloid with proper auxiliary agent according to certain proportion, wherein the mass percentage of the main medicine and the auxiliary agent respectively accounts for 50 percent to 90 percent and 10 percent to 50 percent. The compound insecticide has the advantagesthat the compound insecticide is broad-spectrum, has control effect to various crop pests, and particularly has obvious effect to homoptera pets, lepidoptera pets and coleopteran pets; the desinsection speed is high; the lasting period is long; pets are difficult to develop drug resistance; the compound insecticide is safe to people and animals, environment and natural enemies; the manufacturing process is simple, the material sources are wide, and the cost is low; and the preparations are multiple, and the application field is wide.

The invention discloses a rice field base application multi-effect nutrient, which comprises the following components in percentage by weight: 43.17 to 58.4 percent of nitrogen fertilizer, 18.18 to 27.27 percent of phosphorus fertilizer, 10 to 16.67 percent of potassium fertilizer, 1.5 to 2 percent of zinc sulfate, 3 to 7 percent of sodium silicate, 0.04 to 0.08 percent of compound niclofolan sodium and 6.9 to 8.3 percent of filler. The nitrogen, phosphorus and potassium fertilizers in the components meet the conditions that N is 20 to 25 percent, P2O5 is 8 to 12 percent and K2O is 6 to 10 percent. The rice field base application multi-effect nutrient can meet reasonable nutrition required for the growth of rice, and also can enhance the disease, insect and adversity resistance of the rice, relieve the harm of toxic substances, promote steady and robust growth of the rice and meet the requirements of high yield and good quality.

The invention relates to a biological compound fertilizer capable of promoting growth of crop roots. The biological compound fertilizer is prepared from the following components in parts by weight: 20-40 parts of inorganic composition, 30-50 parts of organic composition, 4-8 parts of composite bacterial powder, 5-10 parts of potassium fulvate, 10-20 parts of ammonium calcium nitrate, 5-15 parts of mannitol, and 5-10 parts of bentonite, wherein the composite bacterial powder is composed of Bacillus subtilis, Bacillus licheniformis, Bacillus laterosporus, photosynthetic bacteria and actinomyces. According to the biological compound fertilizer, based on the mutual action principle of plant-microorganism-soil and active response principle of roots to environment, two aspects of rhizospheric microorganisms and fertilizer nutrients are taken as points of penetration, on the basis of meeting the nitrogen, phosphorus and potassium nutrients needed by crops, the air permeability of the soil is regulated by organic matters, the effective absorption of calcium is promoted by chelating calcium ions with mannitol and potassium fulvate, and meanwhile fully water-soluble potassium fulvate has the function of regulating plant growth, so that extended growth of the crop roots and generation of fibrous roots are stimulated so as to finally achieve the effects of promoting growth and development of the plants and improving the yield.

A Chinese prawn heat shock protein gene 70 is disclosed, which has the SEQ ID No.1 base sequence and SEQ ID No.2 amino acid sequence. Its cloning process includes extracting general RNA from tissue of Chinese prawn, synthesizing the first chain of cDNA, and using a pair of primer for RT-PCR.

The invention provides a method and application of a photosynthetic bacteria culture solution in medicinal material planting. The method comprises the following steps: diluting the photosynthetic bacteria culture solution; and evenly irrigating soil, soaking seeds, irrigating roots and spraying. By utilizing the method, the medicinal material growth can be promoted; the occurrence of various disasters can be prevented and treated; the problem of manual culture quality reduction caused by utilizing chemical fertilizers and pesticides can be solved; the traditional Chinese medicine extract content can be increased; the content of the effective components in the traditional Chinese medicinal material is increased; the heavy metal content is reduced; the utilization of the Chinese medicinal material is improved; the sustainable utilization of the Chinese medicinal material is realized; the resources are protected; the implementation of GAP (good agriculture practice) in China can be improved; the problem that crop rotation can not be performed in Chinese medicinal material planting can be solved; and the fertilizer amount is saved by one third.

The invention belongs to the technical field of biology, and relates to a microbial combined agent for preventing and treating plant diseases and insect pests and promoting plant growth. The microbialcombined preparation is a combined preparation prepared from a quick dissolving microcapsule microbial agent and a bactericidal or insecticidal microorganism slow-release microbial agent according toa ratio of (1-5):(1-9) and used for regulating the growth and development of plants and preventing and treating plant diseases and insect pests, wherein the quick dissolving microcapsule microbial agent is prepared from bacillus amyloliquefaciens, and the bactericidal or insecticidal microorganism slow-release microbial agent is embedded by using a wax film material.

The invention discloses a method for avoiding continuous cropping obstacles of sheltered watermelon, which includes: diluting photosynthetic bacteria culture solution, and performing irrigation and root irrigation for sheltered watermelon. By the method, disease resistance, insect resistance and stress resistance of the sheltered watermelon can be improved, various soil-borne diseases can be inhibited and reduced, healthy growth of the sheltered watermelon is promoted, and yield is increased; pesticide and fertilizer residue and other toxic harmful substances (hydrogen sulfide, organic amines and chlorobenzene) in soil and plants can be degraded, and safety of agricultural products is guaranteed; and nitrogenase activity of watermelon plant rhizosphere can be improved, organic content of the soil is increased effectively under the action of carbon fixation and nitrogen fixation of the watermelon plants, and consumption of fertilizer is reduced. Material sources for the method are extensive and cheap, the problem of failure of continuous cropping in sheltered watermelon culture is solved thoroughly, residue of harmful substances is avoided, and production of pollution-free watermelons is effectively and technically guaranteed.

The invention belongs to the field of agricultural planting technologies, and in particular relates to a method for improving the germination rate of stevia rebaudiana seeds. The method for improving the germination rate of the stevia rebaudiana seeds comprises the following steps: 1) removing pappi; 2) performing cleaning; 3) performing ultraviolet irradiation; 4) soaking the seeds in seed soaking liquid; and 5) performing germination. The method provided by the invention can significantly improve the ability of withstanding adverse situations of the stevia rebaudiana seeds during germination, and enables the stevia rebaudiana seeds to still keep a relatively high germination rate and germination vigor when encountering an adverse situation hazard, thereby achieving an aim of forming high-quality seedlings. The method provided by the invention is simple in operation, low in cost and suitable for promotion.

The invention discloses an adjusting method capable of improving germination of a small-grain vegetable seed. The method comprises the following steps: 1) carefully choosing and weighing to-be-treated seeds; 2) preparing humic acid powder with a particle size of less than 40 meshes; and 3) fully mixing the seeds, the humic acid powder and water according to a weight ratio of 1: 1-2: 1-2 in an aluminum pot, standing the obtained mixture at a temperature of 10 to 20 DEG C for 1 to 7 d, then separating the seeds from humic acid and carrying out dehydration at a temperature of 25 DEG C until water content is less than 7%. According to the invention, the natural agriculture grade humic acid powder is employed as an adjusting medium, and the treated seeds have accelerated seed germination speeds and enhanced stress resisting ability in germination. The method is applicable to small-grain vegetable seeds of green vegetables, amaranth, etc.

The invention relates to a biological foliage fertilizer including a chemical reagent, a biological organic matter, a chelating agent, a penetrating agent and a microbial fermentation liquid; the foliar fertilizer has high content of major elements and complete contents of medium and trace elements, and contains metabolic products generated from microbial fermentation, beneficial microbial flora, the chelating agent, the penetrating agent and a growth regulator, is non-toxic, smelless, safe and reliable, has no side effect, and can significantly improve the yield and quality of crops.

The invention discloses a growth promoting substrate for raising watermelon seedlings. The substrate is prepared by using Trichoderma harzianum T83 as a functional microorganism. When the obtained watermelon seedlings which treated by using the growth promoting substrate is compared with control treated seedlings, the plant height, fresh weight of the overground part, the fresh weight of the underground part, dry weight of the overground part, dry weight of the underground part, stem diameter, root vigor, the content of chlorophyll and the number of fungi, bacteria and actinomycetes of roots are increased by 66.76%, 38.12%, 70.24%, 66.03%, 74.07%, 34.23%, 43.13%, 54.93%, 111.19 times, 1.63 times and 4.38 times separately. Through the high-efficiency growth promoting substrate, final planting of microorganisms can be conducted at the roots of the watermelon seedlings during watermelon seedling cultivation, not only can the survival rate of the transplanted seedlings and the growth speedof the transplanted seedlings be improved, but also the resistance of the watermelon seedlings to stress can be improved; therefore, the substrate has a good application prospect.

The invention proposes a high-quality rice production method of enriching selenium and zinc, reducing cadmium and increasing yield. The method comprises: selecting high-quality rice varieties with rice quality up to a first grade of national standard; adopting artificial seedling transplanting and weeding method without applying pesticides and herbicides in the planting process, and performing cultivation in wide and narrow rows; a fertilization method being that applying base fertilizer when plowing a field, applying seedling raising fertilizer within half a month after transplanting, and nolonger applying chemical fertilizer in a later period; adopting a selenium-zinc combined application method to spray selenium and zinc fertilizer on leaf surfaces at a heading stage and early fillingstage of rice; harvesting when the rice maturity reaches about 95%; processing the rice after storing the rice for 1-2 months; and vacuum-packing the rice, and conducting storing in a cold storage. According to the present invention, specific concentration, dosage and amount ratio are adopted at key time points to carry out precise fertilization in the selenium-zinc combined application technologyof rice, the technology has good effects of enriching selenium and zinc, increasing yield and reducing cadmium on rice, and the nutrition quality, safety quality and production efficiency of the riceare greatly improved.

The invention relates to a desert treating method. The method comprises following steps: mixing biological compound fertilizers, reinforced-processed mushroom-sticks, compound-fermented organic fertilizers and plant seeds according to the proportion and loading them into non-woven fabrics; utilizing a sewing machine to sew into flat soil-stabilizing belts, wherein trilateral or quadrangular grids with the largest side length ranging from 0.5 to 2m enclosed by the soil-stabilizing belts are arrayed continuously in a to-be-treated desert area and arranging fixing devices for fixing the soil-stabilizing belts according to wind power of the desert area; enabling humidity of the soil-stabilizing belts to suitable for germination of plant seeds by means of nature or rainwater and keeping humidity of the soil-stabilizing belts to be within the range for seed germination and growth; and forming grid units by the soil-stabilizing belts as basic soil-stabilizing units. The design of the non-woven fabrics can better store water and keep humidity. The biological compound fertilizers, the compound-fermented organic fertilizers and the reinforced-processed mushroom-sticks can provide sufficient nutrient compositions to plant seeds. The desert treating method is utilized so that desert treating efficiency is effectively increased and greening effect is improved. The desert greening stability in the late phase is good.

The invention provides a cloning method of a peanut chitinase gene J11-AhHevamine-A, and belongs to the technical field of gene cloning. The cloning method has the advantages that a novel primer is adopted in the RACE (rapid-amplification of cDNA (complementary deoxyribonucleic acid) ends) amplification gene full-length process, and a Clontech SMART-RACE kit is used in the RACE process; the selected primer in the RACE amplification gene full-length process can be well combined with peanut seed peel cDNA with an aspergillus flavus-resisting gene, so that the length and integrity of the peanut chitinase gene extracted from the peanut seed peel with the aspergillus flavus-resisting gene are guaranteed; the antifungal characteristic of the extracted peanut chitinase gene can be well maintained; furthermore, the peanut chitinase gene is used for improving the peanut gene, the antifungal characteristic of the peanut can be improved, the stress-resistance ability in the peanut growth processis improved, the aspergillus flavus infection-resisting ability of the peanut is improved, and the expression activity of the chitinase in the peanut gene is guaranteed.

The invention discloses a method for improving soil, and includes a soil conditioner based on the method, and a blended base fertilizer matched with the soil conditioner. The soil conditioner includes, by weight, 2 to 5 parts of a nitrification inhibitor, 1 to 3 parts of polyglutamic acid, 2 to 4 parts of a fertilizer synergist, 2 to 3 parts of clay adsorbent, 3 to 5 parts of fulvic acid, and thebalance being sludge; the blended base fertilizer includes an organic compound fertilizer composed of a magnesium sulfate fertilizer and a phosphorus-potassium compound fertilizer, wherein the percentages of the magnesium sulfate fertilizer and the potassium dihydrogen phosphate fertilizer are 20-40% and 80-60% respectively. The method can improve nitrogen fixation effect and promote development of root system of crops on the soil, can enhance disease resistance and improve disease resistance and stress resistance of the plants, can integrate nutrients in plants and waste and fertilizer components in soil, can improve resistance against diseases caused by soil-borne pathogens, makes plants more effectively utilizing the fertilizer, improves yield and quality of crops, and improves soil quality.

The invention discloses an application of fluridone as a strigolactone inhibitor. The fluridone can be used as a plant strigolactone inhibitor for promoting plant branching, has an obvious yield increasing effect on plants, can be applied to various plants, and is small in environmental pollution. The fluridone reagent is low in price, and can be applied to agricultural production in a large scale.

The invention provides an application method of photosynthetic bacteria culture solution in protected cultivation of melon, which comprises the steps of: diluting the photosynthetic bacteria culture solution, uniformly soaking seeds with the solution, pouring root and spraying. The method can improve the abilities of melon cultured in protected field for resisting diseases, insects and adversity, enhance photosynthetic intensity, and promote healthy growth of the melon; increases content of soluble sugar, improves quality deterioration due to using fertilizers and pesticides during artificial cultivation; degrades residual pesticides and fertilizers, and other toxic and harmful substances in soil and plant, and guarantees security of agriculture products; and effectively increases the content of organic matter in soil and saves 1/3 of fertilizer use amount through carbon fixation and nitrogen fixation of melon itself. The method disclosed in the invention cannot only decrease the use amount of pesticides and fertilizers in protected cultivation of the melon, but also cannot cause residue of any harmful substance, and can satisfy production need of standard melon of green food and organic food.

The invention provides a foliar fertilizer. The foliar fertilizer is prepared from 0.1%-0.9% of 5-aminolevulinic acid, 3%-8% of potassium fulvate, 0.2%-0.3% of sodium citrate, 0.2%-0.3% of trace elements, 1.2%-2.8% of amino acid and the balance of water. 5-aminolevulinic acid is matched with potassium fulvate for use, a small amount of trace elements, sodium citrate and amino acid are used as auxiliary materials, the quick-acting property of the amino acid is exerted, the persistence of potassium fulvate is realized, absorption of trace elements can be promoted in each stage of plant growth, the utilization efficiency of trace elements is improved to the greatest extent, the effects of regulating chlorophyll synthesis and regulating plant growth of 5-aminolevulinic acid are achieved at thesame time, the fertilizer has quick-acting property and persistence, plants are promoted to absorb trace elements in the fertilizer to the maximum extent, the absorption efficiency of the trace elements is improved, the use amount of the trace elements is reduced, resource waste caused by excessive use of the trace elements is avoided, and the crop yield is obviously increased.