Cloning method of peanut chitinase gene J11-AhHevamine-A

A technology of chitinase, cloning method, applied in the field of gene cloning

Inactive Publication Date: 2018-03-02
SHANDONG PEANUT RES INST
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  • Description
  • Claims
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Problems solved by technology

[0007] Although through long-term technical development and technical accumulation, at present, there is no method to find chitinase that can be used to improve the anti-mold characteristics of peanuts. Therefore, it is urgent to develop a peanut chitinase J11-AhHevamine-A gene Cloning method for obtaining chitinase with better integrity and can be used to significantly improve the anti-fungal properties of peanut

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  • Cloning method of peanut chitinase gene J11-AhHevamine-A
  • Cloning method of peanut chitinase gene J11-AhHevamine-A

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[0035] In order to make the purpose, technical solution and advantages of the present application clearer, the implementation manners of the present application will be further described in detail below in conjunction with the accompanying drawings.

[0036] The terms "first", "second", "third", "fourth", "fifth", "sixth", "seventh" and "Eighth", etc., if present, are used to distinguish similar items and are not necessarily used to describe a particular order or sequence. It is to be understood that the data so used are interchangeable under appropriate circumstances such that the embodiments of the invention described herein are, for example, capable of practice in sequences other than those illustrated or described herein.

[0037] Furthermore, the terms "comprising" and "having", as well as any variations thereof, are intended to cover a non-exclusive inclusion, for example, a process, method, system, product or device comprising a sequence of steps or elements is not nece...

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Abstract

The invention provides a cloning method of a peanut chitinase gene J11-AhHevamine-A, and belongs to the technical field of gene cloning. The cloning method has the advantages that a novel primer is adopted in the RACE (rapid-amplification of cDNA (complementary deoxyribonucleic acid) ends) amplification gene full-length process, and a Clontech SMART-RACE kit is used in the RACE process; the selected primer in the RACE amplification gene full-length process can be well combined with peanut seed peel cDNA with an aspergillus flavus-resisting gene, so that the length and integrity of the peanut chitinase gene extracted from the peanut seed peel with the aspergillus flavus-resisting gene are guaranteed; the antifungal characteristic of the extracted peanut chitinase gene can be well maintained; furthermore, the peanut chitinase gene is used for improving the peanut gene, the antifungal characteristic of the peanut can be improved, the stress-resistance ability in the peanut growth processis improved, the aspergillus flavus infection-resisting ability of the peanut is improved, and the expression activity of the chitinase in the peanut gene is guaranteed.

Description

technical field [0001] The embodiment of the present application relates to the technical field of gene cloning, in particular to a method for cloning peanut chitinase J11-AhHevamine-A gene. Background technique [0002] Peanuts, also known as groundnuts, are annual herbaceous plants, known as "vegetable meat" because of their high oil content. In addition to being used for food, peanuts can also be used in printing and dyeing, papermaking industries, etc., and are important oil crops and economic crops in my country. my country's peanut planting area and total production rank among the top in the world, and it has strong international competitiveness. However, my country's current peanut germplasm resources are relatively limited, and some germplasm resources that are resistant to disease and stress are relatively scarce, especially those resistant to Aspergillus flavus. [0003] Although conventional hybrid breeding and mutation breeding can improve the genetics of peanu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1096
Inventor 赵小波李春娟王娟闫彩霞单世华张浩谢宏峰石程仁
Owner SHANDONG PEANUT RES INST
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