The invention relates to a method and a system for evaluation of man-machine work efficiency of a cabin simulation test bed. The system comprises a simulation operating cabin, a lighting device, a photographing device, a display device mounted in the simulation operating cabin and used for simulation of a dashboard, a display device for simulation of a cabin exterior view, a display device for simulation of an operating panel, and a computer system, wherein the lighting device and the photographing device are arranged in the simulation operating cabin. A data acquisition device is arranged around an operator seat which is arranged in the simulation operating cabin, and comprises sensors, an eye movement instrument, an infrared tracking instrument, a 3D (three-dimensional) mouse and a data glove. The display device for simulation of the dashboard, the display device for simulation of the cabin exterior view, the display device for simulation of the operating panel, the operator seat, the sensors, the eye movement instrument, the infrared tracking instrument, the data glove and the 3D glove are all connectedly controlled by the computer. The method and the system for evaluation of man-machine work efficiency of the cabin simulation test bed have the advantage that man-machine work efficiencies under different cabin layouts and information display environments can be compared to realize functions of auxiliary design and man-machine work efficiency examination of each relevant part.