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Application of fluridone as strigolactone inhibitor

A strigolactone and inhibitor technology, applied in the field of pesticides, can solve the problems of high price and no growth regulator of strigolactone inhibitors, achieve low price, improve the ability to resist adversity, and save labor consumption Effect

Active Publication Date: 2021-08-06
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price, there is no growth regulator of strigolactone inhibitors applicable to agricultural production at present.

Method used

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  • Application of fluridone as strigolactone inhibitor
  • Application of fluridone as strigolactone inhibitor
  • Application of fluridone as strigolactone inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The influence of embodiment 1 fluridone solution spray concentration on Arabidopsis branch

[0024] The experiment was repeated three times, and the method of repeating the experiment each time was as follows:

[0025] (1) Material preparation. Arabidopsis was used as a model plant.

[0026] (2) Seed selection and disinfection. The Arabidopsis seeds were sequentially carried out according to the following steps: select Columbia wild-type Arabidopsis seeds, soak and disinfect the seeds with 75% ethanol for 15 minutes; wash with sterilized deionized water for 5 times to obtain Arabidopsis sterilized seeds.

[0027] (3) Seedling growth. The Arabidopsis seeds obtained in step (2) were spot-planted on 1 / 2 MS solid medium, put into a refrigerator at 4°C for vernalization for 3 days, and then placed at 22°C and cultured for 7 days under 16 hours of light and 8 hours of darkness. When Arabidopsis thaliana grows 4 leaves, it is transplanted into small pots (size: 10cm×10cm) ...

Embodiment 2

[0034] The influence of embodiment 2 fluridone spray concentration on Arabidopsis branch

[0035] The experimental steps of the effect of fluridone spraying concentration on Arabidopsis branches are as follows:

[0036] (1) Material preparation. Arabidopsis was used as a model plant.

[0037] (2) Seed selection and disinfection. The Arabidopsis seeds were followed by the following steps: select Columbia wild-type Arabidopsis seeds, use 75% ethanol to soak and disinfect the seeds for 15 minutes; wash 3-5 times with sterilized deionized water to obtain Arabidopsis sterilized seed.

[0038] (3) Seedling growth. The Arabidopsis seeds obtained in step (2) were spot-planted on 1 / 2 MS solid medium, put into a refrigerator at 4°C for vernalization for 3 days, and then placed at 22°C and cultured for 7 days under 16 hours of light and 8 hours of darkness. When four leaves of A. thaliana grow, they are transplanted into small pots (size: 10cm×10cm) for cultivation, and 5 plants are...

Embodiment 3

[0044] The influence of embodiment 3 fluridone spray concentration on the growth of Arabidopsis

[0045] The Arabidopsis plants sprayed with different concentrations of fluridone reagent ((0.01, 0.05, 0.1, 0.5, 1 μM) and control group CK) in Example 1 were detected for one week, and their photosynthetic intensity under different concentrations were measured. and physiological indicators.

[0046]Photosynthetic intensity measurement: use the LI-COR / LI-6400 portable photosynthetic measurement system to measure and output photosynthetic intensity indicators.

[0047] The results of different concentrations of fluridone solution on the leaves of Arabidopsis are as follows: figure 2 As shown, it can be seen from the figure that the leaves of Arabidopsis thaliana in the fluridone solution with a concentration of 0.1-1 μM all have a certain degree of yellowing. The results of photosynthetic intensity were measured as image 3 As shown in the figure, it can be seen from the figure...

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Abstract

The invention discloses an application of fluridone as a strigolactone inhibitor. The fluridone can be used as a plant strigolactone inhibitor for promoting plant branching, has an obvious yield increasing effect on plants, can be applied to various plants, and is small in environmental pollution. The fluridone reagent is low in price, and can be applied to agricultural production in a large scale.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and in particular relates to the application of fluridone as a strigolactone inhibitor. Background technique [0002] Strigolactones are a plant hormone or its precursor discovered in recent years, which can inhibit the growth of plant branches and lateral buds, and it cooperates with auxin and cytokinin to control plant branches (tillers) quantity. As a carotenoid derivative produced in plant roots, strigolactone promotes symbiosis between plants and soil microbes. In addition, during the interaction between plants and their parasitic plants, strigolactones also act as signaling molecules, which can induce parasitic plants such as Striga spp., Orobanche spp. germination. [0003] Due to the functional characteristics of strigolactone, it has become a new means of regulating plant branching, directly or indirectly inhibiting the germination of plant lateral buds to produce branches, regulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/40A01P21/00A01G7/06A01G13/00
CPCA01N43/40A01G7/06A01G13/00Y02A40/10
Inventor 肖浪涛苏益寻敏
Owner HUNAN AGRICULTURAL UNIV
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