The invention discloses a Chinese herbal medicine enzyme for culture and a preparation method thereof, wherein the Chinese herbal medicine enzyme is prepared from the following raw materials in partsby weight: 8-12 parts of radix isatidis, 10-15 parts of Chinese pulsatilla root, 10-18 parts of a fly maggot enzyme liquid, 4-8 parts of fructus forsythiae, 3-6 parts of folium eucalypti, 1-7 parts ofhoneysuckle, 9-16 parts of rhizoma acori graminei, 6-10 parts parts of zornia gibbosa, 4-8 parts of gentiana scabra bge, 8-12 parts of selenium-rich yeast, 2-6 parts of an enzyme group, 10-18 parts of brown sugar and 30-50 parts of water. The Chinese herbal enzyme product has no drug residues, has strong inhibitory effect on pathogenic bacteria and viruses, is safe and high in efficiency, is moreeasily absorbed and utilized by cultured animals, has good prevention, improvement and control effects on enteritis, anorexia, solitary bacterial infection, epidemic viruses and the like, and reallyplays roles in eliminating pernicious influence, diminishing inflammation, inhibiting bacteria, repelling insect, removing stinking flavor, removing odor, strengthening intestines, stimulating appetite, attracting food, preventing stress and resisting virus, enhancing immunity and disease resistance, effectively reducing the risk of breeding, and effectively improving the yield and the success rate of breeding.