The invention discloses a truffle wine and a preparation method of the truffle wine. The truffle wine comprises the following raw materials by weight: 900-1000 parts of base wine, 10-15 parts of blacktruffle, 8-10 parts of silkworm chrysalis, 1-10 parts of cortex acanthopanacis, 0.1-1 part of cultivated ginseng, 0.5-1 part of chrysanthemum, 0.1-0.5 part of Chinese angelica, 0.5-1 part of codonopsis pilosula, 0.5-1 part of schisandra chinensis, 0.3-0.7 part of radix puerariae, 1-3 parts of walnut meat, 0.2-3 parts of ligusticum wallichii, 0.1-0.5 part of honey, and 0.1-0.5 part of rock candy.The truffle wine has no medicinal odor and has an aromatic flavor, has the effects of nourishing yin, tonifying qi, cooling down, refreshing, restoring consciousness and the like, has a sweet aftertaste after being drunk, and makes people feel refreshed. The preparation method of the truffle wine is performed at a low temperature, so that the effective ingredient of the raw material is barely lost, the white spirit and the white wine are taken as the base wine, the rock candy and the honey are used to adjust the flavor, the taste is better, the effect is more obvious, and the nutrient is richer.