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145 results about "OMEGA-3 POLYUNSATURATED FATTY ACIDS" patented technology

The Function of Omega-3 Polyunsaturated Fatty Acids. ω-3 PUFA is one of the four classes of fatty acids, which include Omega-9, Omega-6 and saturated fats. “Fatty acids play a role in almost every cell in the body,” notes Dr. Lavie.

Method for producing DHA by Schizochytrium in high-density culture through fermentation

The invention discloses a method for producing DHA by Schizochytrium in high-density culture through fermentation, relates to a preparation method of DHA, and provides a method for producing DHA by using a microbial fermentation method, which replaces fish oil to extract omega-3 polyunsaturated fatty acids. The method comprises the following steps of: inoculating strains in a shake flask according to the ratio of strains to a culture medium of 1: (5-10) for culturing until the dry weight of cells in fermentation liquor is 10-12g / L; inoculating the strains in the shake flask in a 1000L seed fermenter according to the inoculum size of 5-10%, culturing until the dry weight of the cells in the fermentation liquor is 20-24g / L; inoculating the strains in the 1000L seed fermenter in a 8000L seed fermenter according to the inoculum size of 5-10%, and culturing for 120-130 hours, wherein the dry weight of the cells in the fermentation liquor is 120-150g / L, and the DHA content is up to 26-30g / L. In the invention, through optimizing the culture medium and optimizing the culture conditions and by adopting a conventional fermentation method, the production rate of the DHA is at least 3 times more than the reported production rate. The production costs of the strains (the Schizochytrium), the culture medium and the culture condition are low, the yield is high, and the method is applicable to the industrial production of the DHA.
Owner:XIAMEN HUISON BIOTECH

Microalgae extract containing omega 3-polyunsaturated fatty acids and method for extracting oil from micro-organisms

The present invention relates to a biphasic microalgae extract comprising 15-95 wt. % of an aqueous phase and 5-85 wt. % of an oil phase, said extract containing at least 1% by weight of total fatty acids of ω3-fatty acids selected from the group consisting of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and combinations thereof, said extract further being characterized in that it contains: • 40-100% by weight of the oil phase of lipids selected from triglycerides, diglycerides, monoglycerides, phosphatides, free fatty acids and combinations thereof; • 0.01-10% of carotenoids by weight of the oil phase; • 0.5-10% of sodium chloride by weight of the aqueous phase; • 0-3 wt. % of non-dissolved material other than dispersed droplets of aqueous phase or oil phase; and • 0-10% of C1-5 monoalcohol by weight of the aqueous phase. Together the aqueous phase and oil phase of the biphasic extract contain essentially all the lipid material that was originally contained in the microalgae biomass, i.e. both apolar lipids (e.g. triglycerides, free fatty acids) and polar lipids (e.g. glycolipids, phospholipids). The invention also provides processes for extracting an oil phase from wet biomass of micro-organisms. These processes do not require the use of apolar organic solvents and produce a biphasic extract that can easily be downstream processed to produce ω3-PUFA containing oil in high yield.
Owner:GONZALEZ RAMON NIEVES

Method for producing DHA by Schizochytrium in high-density culture through fermentation

The invention discloses a method for producing DHA by Schizochytrium in high-density culture through fermentation, relates to a preparation method of DHA, and provides a method for producing DHA by using a microbial fermentation method, which replaces fish oil to extract omega-3 polyunsaturated fatty acids. The method comprises the following steps of: inoculating strains in a shake flask according to the ratio of strains to a culture medium of 1: (5-10) for culturing until the dry weight of cells in fermentation liquor is 10-12g / L; inoculating the strains in the shake flask in a 1000L seed fermenter according to the inoculum size of 5-10%, culturing until the dry weight of the cells in the fermentation liquor is 20-24g / L; inoculating the strains in the 1000L seed fermenter in a 8000L seed fermenter according to the inoculum size of 5-10%, and culturing for 120-130 hours, wherein the dry weight of the cells in the fermentation liquor is 120-150g / L, and the DHA content is up to 26-30g / L. In the invention, through optimizing the culture medium and optimizing the culture conditions and by adopting a conventional fermentation method, the production rate of the DHA is at least 3 times more than the reported production rate. The production costs of the strains (the Schizochytrium), the culture medium and the culture condition are low, the yield is high, and the method is applicable to the industrial production of the DHA.
Owner:XIAMEN HUISON BIOTECH

Novel vector for raising content of polyunsaturated fatty acids in animal body

The invention discloses a novel vector for raising the content of polyunsaturated fatty acids in an animal body. The expression vector provided by the invention can simultaneously transcribe and express omega-3 fatty acid dehydrogenase and delta12 fatty acid dehydrogenase, and the vector sequence contains gene sequences of Sequence NO.1 and Sequence NO.2. In addition, the sequences of the enhancer SP163 and IRES are added between the sFat and Fat2 genes, and the vector provided by the invention can highly express two types of dehydrogenase at the same time. The vector provided by the invention is also transfected into the CHO cells, followed by the screening of antibiotics to obtain a stable cell line for highly expressing the sFat and Fat2 genes with the gene expression amount being 105 times that of untransfected cells. The invention also discloses a concrete method for raising offspring animals having high content of polyunsaturated fatty acids by using the vector. The vector provided by the invention lays a foundation for producing animal products having high content of omega-3 polyunsaturated fatty acids and improving the quality of meat products or dairy products by producing transgenic livestock.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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